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Raquel Chaves - One of the best experts on this subject based on the ideXlab platform.
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Hidden heterochromatin: Characterization in the Rodentia species Cricetus Cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae)
2016Co-Authors: Ana Paco, Filomena Adega, Henrique Guedes-pinto, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin re-vealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when com-pared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rear-rangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes. Key words: constitutive heterochromatin, in situ restriction endonuclease digestion, Rodentia
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A High-Resolution Comparative Chromosome Map of Cricetus Cricetus and Peromyscus eremicus Reveals the Involvement of Constitutive Heterochromatin in Breakpoint Regions
Cytogenetic and genome research, 2015Co-Authors: A. Vieira-da-silva, Filomena Adega, Sandra Louzada, Raquel ChavesAbstract:Compared to humans and other mammals, rodent genomes, specifically Muroidea species, underwent intense chromosome reshuffling in which many complex structural rearrangements occurred. This fact makes them preferential animal models for studying the process of karyotype evolution. Here, we present the first combined chromosome comparative maps between 2 Cricetidae species, Cricetus Cricetus and Peromyscus eremicus, and the index species Mus musculus and Rattus norvegicus. Comparative chromosome painting was done using mouse and rat paint probes together with in silico analysis from the Ensembl genome browser database. Hereby, evolutionary events (inter- and intrachromosomal rearrangements) that occurred in C. Cricetus and P. eremicus since the putative ancestral Muroidea genome could be inferred, and evolutionary breakpoint regions could be detected. A colocalization of constitutive heterochromatin and evolutionary breakpoint regions in each genome was observed. Our results suggest the involvement of constitutive heterochromatin in karyotype restructuring of these species, despite the different levels of conservation of the C. Cricetus (derivative) and P. eremicus (conserved) genomes.
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Copyright © 2009, Sociedade Brasileira de Genética. Printed in Brazil www.sbg.org.br Hidden heterochromatin: Characterization in the Rodentia species Cricetus
2013Co-Authors: Peromyscus Eremicus, Filomena Adega, Ana Paco, Henrique Guedes-pinto, Praomys Tullbergi, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes. Key words: constitutive heterochromatin, in situ restriction endonuclease digestion, Rodentia
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hidden heterochromatin characterization in the rodentia species Cricetus Cricetus peromyscus eremicus cricetidae and praomys tullbergi muridae
Genetics and Molecular Biology, 2009Co-Authors: Ana Paco, Filomena Adega, Henrique Guedespinto, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes.
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different evolutionary trails in the related genomes Cricetus Cricetus and peromyscus eremicus rodentia cricetidae uncovered by orthologous satellite dna repositioning
Micron, 2008Co-Authors: Ana Paco, Filomena Adega, Henrique Guedespinto, Svatava Kubickova, Jiri Rubes, Raquel ChavesAbstract:Constitutive heterochromatin comprises a substantial fraction of the eukaryotic genomes and is mainly composed of tandemly arrayed satellite DNAs (satDNA). These repetitive sequences represent a very dynamic and fast evolving component of genomes. In the present work we report the isolation of Cricetus Cricetus (CCR, Cricetidae, Rodentia) centromeric repetitive sequences from chromosome 4 (CCR4/10sat), using the laser microdissection and laser pressure catapulting procedure, followed by DOP-PCR amplification and labelling. Physical mapping by fluorescent in situ hybridisation of these sequences onto C. Cricetus and another member of Cricetidae, Peromyscus eremicus, displayed quite interesting patterns. Namely, the centromeric sequences showed to be present in another C. Cricetus chromosome (CCR10) besides CCR4. Moreover, these almost chromosome-specific sequences revealed to be present in the P. eremicus genome, and most interestingly, displaying a ubiquitous scattered distribution throughout this karyotype. Finally and in both species, a co-localisation of CCR4/10sat with constitutive heterochromatin was found, either by classical C-banding or C-banding sequential to in situ endonuclease restriction. The presence of these orthologous sequences in both genomes is suggestive of a phylogenetic proximity. Furthermore, the existence of common repetitive DNA sequences with a different chromosomal location foresees the occurrence of an extensive process of karyotype restructuring somehow related with intragenomic movements of these repetitive sequences during the evolutionary process of C. Cricetus and P. eremicus species.
Ana Paco - One of the best experts on this subject based on the ideXlab platform.
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Hidden heterochromatin: Characterization in the Rodentia species Cricetus Cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae)
2016Co-Authors: Ana Paco, Filomena Adega, Henrique Guedes-pinto, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin re-vealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when com-pared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rear-rangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes. Key words: constitutive heterochromatin, in situ restriction endonuclease digestion, Rodentia
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Copyright © 2009, Sociedade Brasileira de Genética. Printed in Brazil www.sbg.org.br Hidden heterochromatin: Characterization in the Rodentia species Cricetus
2013Co-Authors: Peromyscus Eremicus, Filomena Adega, Ana Paco, Henrique Guedes-pinto, Praomys Tullbergi, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes. Key words: constitutive heterochromatin, in situ restriction endonuclease digestion, Rodentia
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hidden heterochromatin characterization in the rodentia species Cricetus Cricetus peromyscus eremicus cricetidae and praomys tullbergi muridae
Genetics and Molecular Biology, 2009Co-Authors: Ana Paco, Filomena Adega, Henrique Guedespinto, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes.
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different evolutionary trails in the related genomes Cricetus Cricetus and peromyscus eremicus rodentia cricetidae uncovered by orthologous satellite dna repositioning
Micron, 2008Co-Authors: Ana Paco, Filomena Adega, Henrique Guedespinto, Svatava Kubickova, Jiri Rubes, Raquel ChavesAbstract:Constitutive heterochromatin comprises a substantial fraction of the eukaryotic genomes and is mainly composed of tandemly arrayed satellite DNAs (satDNA). These repetitive sequences represent a very dynamic and fast evolving component of genomes. In the present work we report the isolation of Cricetus Cricetus (CCR, Cricetidae, Rodentia) centromeric repetitive sequences from chromosome 4 (CCR4/10sat), using the laser microdissection and laser pressure catapulting procedure, followed by DOP-PCR amplification and labelling. Physical mapping by fluorescent in situ hybridisation of these sequences onto C. Cricetus and another member of Cricetidae, Peromyscus eremicus, displayed quite interesting patterns. Namely, the centromeric sequences showed to be present in another C. Cricetus chromosome (CCR10) besides CCR4. Moreover, these almost chromosome-specific sequences revealed to be present in the P. eremicus genome, and most interestingly, displaying a ubiquitous scattered distribution throughout this karyotype. Finally and in both species, a co-localisation of CCR4/10sat with constitutive heterochromatin was found, either by classical C-banding or C-banding sequential to in situ endonuclease restriction. The presence of these orthologous sequences in both genomes is suggestive of a phylogenetic proximity. Furthermore, the existence of common repetitive DNA sequences with a different chromosomal location foresees the occurrence of an extensive process of karyotype restructuring somehow related with intragenomic movements of these repetitive sequences during the evolutionary process of C. Cricetus and P. eremicus species.
Filomena Adega - One of the best experts on this subject based on the ideXlab platform.
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Hidden heterochromatin: Characterization in the Rodentia species Cricetus Cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae)
2016Co-Authors: Ana Paco, Filomena Adega, Henrique Guedes-pinto, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin re-vealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when com-pared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rear-rangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes. Key words: constitutive heterochromatin, in situ restriction endonuclease digestion, Rodentia
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A High-Resolution Comparative Chromosome Map of Cricetus Cricetus and Peromyscus eremicus Reveals the Involvement of Constitutive Heterochromatin in Breakpoint Regions
Cytogenetic and genome research, 2015Co-Authors: A. Vieira-da-silva, Filomena Adega, Sandra Louzada, Raquel ChavesAbstract:Compared to humans and other mammals, rodent genomes, specifically Muroidea species, underwent intense chromosome reshuffling in which many complex structural rearrangements occurred. This fact makes them preferential animal models for studying the process of karyotype evolution. Here, we present the first combined chromosome comparative maps between 2 Cricetidae species, Cricetus Cricetus and Peromyscus eremicus, and the index species Mus musculus and Rattus norvegicus. Comparative chromosome painting was done using mouse and rat paint probes together with in silico analysis from the Ensembl genome browser database. Hereby, evolutionary events (inter- and intrachromosomal rearrangements) that occurred in C. Cricetus and P. eremicus since the putative ancestral Muroidea genome could be inferred, and evolutionary breakpoint regions could be detected. A colocalization of constitutive heterochromatin and evolutionary breakpoint regions in each genome was observed. Our results suggest the involvement of constitutive heterochromatin in karyotype restructuring of these species, despite the different levels of conservation of the C. Cricetus (derivative) and P. eremicus (conserved) genomes.
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Copyright © 2009, Sociedade Brasileira de Genética. Printed in Brazil www.sbg.org.br Hidden heterochromatin: Characterization in the Rodentia species Cricetus
2013Co-Authors: Peromyscus Eremicus, Filomena Adega, Ana Paco, Henrique Guedes-pinto, Praomys Tullbergi, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes. Key words: constitutive heterochromatin, in situ restriction endonuclease digestion, Rodentia
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hidden heterochromatin characterization in the rodentia species Cricetus Cricetus peromyscus eremicus cricetidae and praomys tullbergi muridae
Genetics and Molecular Biology, 2009Co-Authors: Ana Paco, Filomena Adega, Henrique Guedespinto, Raquel ChavesAbstract:The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus Cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes.
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different evolutionary trails in the related genomes Cricetus Cricetus and peromyscus eremicus rodentia cricetidae uncovered by orthologous satellite dna repositioning
Micron, 2008Co-Authors: Ana Paco, Filomena Adega, Henrique Guedespinto, Svatava Kubickova, Jiri Rubes, Raquel ChavesAbstract:Constitutive heterochromatin comprises a substantial fraction of the eukaryotic genomes and is mainly composed of tandemly arrayed satellite DNAs (satDNA). These repetitive sequences represent a very dynamic and fast evolving component of genomes. In the present work we report the isolation of Cricetus Cricetus (CCR, Cricetidae, Rodentia) centromeric repetitive sequences from chromosome 4 (CCR4/10sat), using the laser microdissection and laser pressure catapulting procedure, followed by DOP-PCR amplification and labelling. Physical mapping by fluorescent in situ hybridisation of these sequences onto C. Cricetus and another member of Cricetidae, Peromyscus eremicus, displayed quite interesting patterns. Namely, the centromeric sequences showed to be present in another C. Cricetus chromosome (CCR10) besides CCR4. Moreover, these almost chromosome-specific sequences revealed to be present in the P. eremicus genome, and most interestingly, displaying a ubiquitous scattered distribution throughout this karyotype. Finally and in both species, a co-localisation of CCR4/10sat with constitutive heterochromatin was found, either by classical C-banding or C-banding sequential to in situ endonuclease restriction. The presence of these orthologous sequences in both genomes is suggestive of a phylogenetic proximity. Furthermore, the existence of common repetitive DNA sequences with a different chromosomal location foresees the occurrence of an extensive process of karyotype restructuring somehow related with intragenomic movements of these repetitive sequences during the evolutionary process of C. Cricetus and P. eremicus species.
Karsten Neumann - One of the best experts on this subject based on the ideXlab platform.
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molecular phylogeny of the cricetinae subfamily based on the mitochondrial cytochrome b and 12s rrna genes and the nuclear vwf gene
Molecular Phylogenetics and Evolution, 2006Co-Authors: Karsten Neumann, Johan Michaux, V S Lebedev, Nuri Yigit, Ercument Colak, Natalia Ivanova, Andrey Poltoraus, A V Surov, Georgi Markov, Steffen MaakAbstract:Despite some popularity of hamsters as pets and laboratory animals there is no reliable phylogeny of the subfamily Cricetinae available so far. Contradicting views exist not only about the actual number of species but also concerning the validity of several genera. We used partial DNA sequences of two mitochondrial (cytochrome b, 12S rRNA) and one partial nuclear gene (von Willebrand Factor exon 28) to provide a first gene tree of the Cricetinae based on 15 taxa comprising six genera. According to our data, Palaearctic hamsters fall into three distinct phylogenetic groups: Phodopus, MesoCricetus, and Cricetus-related species which evolved during the late Miocene about 7–12 MY ago. Surprisingly, the genus Phodopus, which was previously thought to have appeared during the Pleistocene, forms the oldest clade. The largest number of extant hamster genera is found in a group of Cricetus-related hamsters. The genus Cricetulus itself proved to be not truly monophyletic with Cricetulus migratorius appearing more closely related to Tscherskia, Cricetus, and Allocricetulus. We propose to place the species within a new monotypic genus. Molecular clock calculations are not always in line with the dating of fossil records. DNA based divergence time estimates as well as taxonomic relationships demand a reevaluation of morphological characters previously used to identify fossils and extant hamsters.
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genetic spatial structure of european common hamsters Cricetus Cricetus a result of repeated range expansion and demographic bottlenecks
Molecular Ecology, 2005Co-Authors: Karsten Neumann, Johan Michaux, Steffen Maak, H A H Jansman, A Kayser, G Mundt, Rolf GattermannAbstract:The spatial genetic structure of common hamsters (Cricetus Cricetus) was investigated using three partial mitochondrial (mt) genes and 11 nuclear microsatellite loci. All marker systems revealed significant population differentiation across Europe. Hamsters in central and western Europe belong largely to two allopatric mitochondrial lineages south and northwest of the Carpathian and Sudetes. The southern group, 'Pannonia', comprises populations inside the Carpathian basin (Czech Republic, Hungary) while the second group, 'North', includes hamsters from Belgium, the Netherlands, France, and Germany. Isolation of the lineages is maintained by a combination of geographical and ecological barriers. Both main phylogeographical groups show signs of further subdivision. North is separated into highly polymorphic central German and less polymorphic western populations, which most likely split during late glacial expansion (15 00010 000 bp). Clock estimates based on haplotype distributions predict a divergence of the two major lineages 85 000147 000 bp. Expansion times fall during the last glaciation (115 00010 000 bp) corroborating fossil data, which identify Cricetus Cricetus as characteristic of colder climatic phases. Despite the allopatry of mt haplotypes, there is an overlap of nuclear microsatellite alleles between phylogeographical units. Although there are strong evidence that Pannonian hamsters have persisted inside the Carpathian basin over the last 50 000 years, genetic differentiation among European hamsters has mainly been caused by immigration from different eastern refugia. Possible source populations are likely to be found in the Ukrainian and the southern Russian plains core areas of hamster distribution. From there, hamsters have repeatedly expanded during the Quaternary.
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multiple bottlenecks in threatened western european populations of the common hamster Cricetus Cricetus l
Conservation Genetics, 2004Co-Authors: Karsten Neumann, Steffen Maak, H A H Jansman, A Kayser, Rolf GattermannAbstract:Common hamsters Cricetus Cricetus (L.)show a highly fragmented distributionpattern across Europe. Over the last decades,human influence caused significant populationdeclines in particular at the western rangeboundary. Despite the initiation of breedingand release programs the genetic structure anddiversity of European common hamsterpopulations is largely unknown. In this study,hamsters from ten localities in five Europeancountries were investigated. Mitochondrialcontrol region was sequenced from 145 animalsrepresenting all sampled populations. 385hamster were screened for polymorphisms at 11microsatellite loci. Both marker systemsrevealed extensive genetic differentiationamong European common hamsters. Westernpopulations displayed very low levels of mtDNAdiversity (H = 0 − 0.2, Alsace, Limburg,Flanders, Baden-Wuerttemberg) compared toeastern populations from Saxony-Anhalt,Thuringia and Southern Moravia (H = 0.663− 0.816). Microsatellite analyses revealed asimilar pattern with low to moderate diversityvalues in western hamsters (A = 1.636 −5.364; He = 0.111 − 0.504) and highlevels of polymorphism in eastern hamsters(A = 8.909 − 9.818; He = 0.712− 0.786). High microsatellite based FSTmeasures (up to 0.635) suggest a typical islandmodel of distribution with no current gene flowbetween most areas. Western hamster populationsexhibit obvious similarities in mitochondrialhaplotype and microsatellite alleledistributions. Gene trees group westernhamsters consistently together on the samebranch but bootstrap values never reachedsignificance. There are strong indications thatlow diversity in western populations ispartially caused by a joint historic founderevent and not only by recent population breakdowns. Overlapping mitochondrial haplotypesprove a close association between westernhamsters and animals from the east German rangein the recent past which does not support theexistence of a separate subspecies C. c.canescens in Europe. Hamsters from southernMoravia emerged as the genetically mostdistinguished population and could be part of a different genetic lineage in Europe.
A V Surov - One of the best experts on this subject based on the ideXlab platform.
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circle of life the common hamster Cricetus Cricetus adaptations to the urban environment
Integrative Zoology, 2019Co-Authors: A V Surov, Pavel L Bogomolov, Alexandra S. Sayan, N. N. Tovpinetz, Elena A Zaytseva, Alexandr V Kuptsov, Elena A Katzman, Ekaterina V Potashnikova, Ekaterina V Kuznetsova, Alexey Yu TsellariusAbstract:Traditionally, urbanization has been seen as a negative phenomenon for biota. However, changes in the environmental parameters induced by urbanization might be favorable for some species. Over the past half-century, the common hamster has actively populated cities, establishing populations in some European, Russian and Kazakhstan cities. Based on integrative methods, we investigated free-range common hamsters inhabiting Simferopol from 2015 to 2018 to reveal possible adaptations to the urbanized environment across several parameters, including lifespan, hibernation period, reproductive activity and body mass. Results show that in urban areas, the common hamster demonstrates an extremely short hibernation period compared to other localities, possibly due to enhanced food resources from urban forestry (walnuts, locus and hazelnut), allowing the species to start breeding very early (February) and finish as late as October. We present the first evidence of polyandry for this species: mating of receptive females with several males and subsequent confirmation of multiple paternity. Despite high reproductive potential, the lifespan of the common hamster in urban conditions is generally very short (less than 1 year). We speculate that in the process of synurbization, the common hamster's innate plasticity across many life history traits permits it to successfully colonize throughout a wide range of habitats, with the ability to form novel adaptations to urban environments.
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genetic structure of urban and suburban populations of common hamster Cricetus Cricetus in ciscaucasia
Russian Journal of Genetics, 2019Co-Authors: Yu N Feoktistova, Pavel L Bogomolov, Ilya G. Meschersky, Natalia S. Poplavskaya, Sergey I Meschersky, M M Chunkov, V V Yufereva, V A Telpov, A V SurovAbstract:On the basis of the allelic composition of ten microsatellite loci and mtDNA sequences, we studied the genetic structure of the common hamster populations that inhabit cities and suburban settlements in Ciscaucasia. It was shown that, in urbanized territories, the hamster population is divided into separated groups, the genetic differences between which are high and not related to the distance between them. On the outskirts of cities adjacent to undeveloped or green areas and further in rural areas, the degree of isolation of neighboring hamster settlements decreases, and the level of genetic differences between them begins to correspond to the distance separating the settlements. In the course of work, this species was first registered within the territory of the easternmost part of Ciscaucasia, in the Republic of Dagestan.
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к вопросу о границе между двумя филогруппами обыкновенного хомяка Cricetus Cricetus cricetinae rodentia
Povolzhskiy Journal of Ecology, 2018Co-Authors: Natalia Yu Feoktistova, Pavel L Bogomolov, A V Surov, Ilya G. Meschersky, Alexandra S. Sayan, Sergey I Meschersky, Elena F Sitnikova, Andrey A Vlasov, Olga P Vlasova, State Nature Biosphere Reserve Bryansky LesAbstract:The Common hamster ( Cricetus Cricetus ) is the rodent with one of the largest range (6 million km 2 ). There were four phylogenetic lineages earlier recovered in Western Europe, Ukraine and Bryansk Province of Russia: “North”, “Рannonia”, E1 and E0. E1 was previously reported from South-Eastern Poland and Western Ukraine and never been found in sympatry with “Pannonia” although the closest distance between them was estimated as 20 km. The question is whether the sympatry of E1 and E0 phylogroups exists? Special survey was arranged across Moscow, Tula, Bryansk, Oryol, Kursk, Voronezh and Lipetsk provinces to get the answer. Sequence analysis of the mtDNA control region and the cyt b gene from the tissue samples was carried out in the common hamsters captured in these areas and their belonging to a certain phylogroup was determined. For the first time a case of sympatry was revealed – in the city ofMtsensk vicinity (Oryol province). Here we discovered hamsters the both lineages – E0 and E1 at the same habitat. Hypothetically E1/E0 ranges boundary runs from Ukrainian Sumy province to North-East by line dividing the Russian Kursk and Oryol provinces. The existence of subclades within both E1 and E0 phylogroups suggests that diversification of phylogenetic lineages of the Common hamster in Eastern Europe may result from not single but multiple climatic events during the second half of Late Pleistocene. The phylogeographic structure of the species inEastern Europe may be more complex than it currently known.
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Divergence time (kya) between the Common hamsters phylogenetic lineages as evaluated based on suggested (1) Tscherskia triton and (Cricetulus migratorius + Allocricetulus eversmanni + Cricetus Cricetus) clade and (2) Pannonia and North-type lineages
2017Co-Authors: Natalia Yu Feoktistova, Pavel L Bogomolov, Ilya G. Meschersky, Alexandra S. Sayan, Natalia S. Poplavskaya, A V SurovAbstract:Divergence time (kya) between the Common hamsters phylogenetic lineages as evaluated based on suggested (1) Tscherskia triton and (Cricetulus migratorius + Allocricetulus eversmanni + Cricetus Cricetus) clade and (2) Pannonia and North-type lineages separation time.
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Genetic structure of urban population of the common hamster (Cricetus Cricetus)
Russian Journal of Genetics, 2016Co-Authors: Natalia Yu Feoktistova, A V Surov, Ilya G. Meschersky, P. L. Bogomolov, N. N. Tovpinetz, Natalia S. PoplavskayaAbstract:Over the past half-century, the common hamster ( Cricetus Cricetus ), along with range-wide decline of natural populations, has actively populated the cities. The study of the genetic structure of urban populations of common hamster may shed light on features of the habitation of this species in urban landscapes. This article is focused on the genetic structure of common hamster populations in Simferopol (Crimea), one of the largest known urban populations of this species. On the basis of the analysis of nucleotide sequences of the cytochrome b gene and mtDNA control region, and the allelic composition of ten microsatellite loci of nDNA, we revealed that, despite the fact that some individuals can move throughout the city at considerable distances, the entire population of the city is represented by separate demes confined to different areas. These demes are characterized by a high degree of the genetic isolation and reduced genetic diversity compared to that found for the city as a whole.