The Experts below are selected from a list of 993 Experts worldwide ranked by ideXlab platform
William M Wintermantel - One of the best experts on this subject based on the ideXlab platform.
-
Natural Infection Rate of Known Tomato Chlorosis Virus-Susceptible Hosts and the Influence of the Host Plant on the Virus Relationship With Bemisia tabaci MEAM1.
Plant disease, 2020Co-Authors: Arnaldo Esquivel-fariña, William M Wintermantel, Jorge Alberto Marques Rezende, Laura Jenkins Hladky, Daiana BampiAbstract:Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) was identified in tomato crops in the state of Sao Paulo, Brazil in 2006. Management strategies to control external sources of inoculum are necessary, because chemical control of the whitefly vector Bemisia tabaci MEAM1 has not efficiently prevented virus infections and no commercial tomato cultivars or hybrids are resistant to this Crinivirus. We first evaluated the natural infection rate of some known wild and cultivated ToCV-susceptible hosts and their attractiveness for B. tabaci MEAM1 oviposition. Physalis angulata was the most susceptible to natural infection in all six exposures in 2018 and 2019. No plants of Capsicum annuum (cv. Dahra) or Chenopodium album became infected. Solanum melongena (cv. Napoli) had only two infected plants of 60 exposed. C. annuum and C. album were the least preferred, and Nicotiana tabacum and S. melongena were the most preferred for whitefly oviposition. In addition, from 2016 to 2019, we surveyed different tomato crops and the surrounding vegetation to identify ToCV in weeds and cultivated plants in the region of Sumare, Sao Paulo state. Only Solanum americanum, vila vila (S. sisymbriifolium) and C. album were found naturally infected, with incidences of 18%, 20% and 1.4%, respectively. Finally, we estimated the ToCV titer (isolates ToCV-FL, USA and ToCV-SP, Brazil) by RT-qPCR in different ToCV-susceptible host plants and evaluated the relationship between virus acquisition and transmission by B. tabaci MEAM1. The results clearly showed significant differences in ToCV concentrations in the tissues of ToCV-susceptible host plants, which appeared to be influenced by the virus isolate. The concentration of the virus in plant tissues, in turn, directly influenced the ToCV-B. tabaci MEAM1 relationship and subsequent transmission to tomato plants. To minimize or prevent the damage from the tomato yellowing disease through management of external sources of ToCV, it is necessary to correctly identify the potentially important ToCV-susceptible hosts in the vicinity of new plantings.
-
Criniviruses infecting vegetable crops
Applied Plant Biotechnology for Improving Resistance to Biotic Stress, 2020Co-Authors: Varvara I. Maliogka, William M Wintermantel, C. G. Orfanidou, Nikolaos I. KatisAbstract:Abstract Criniviruses are associated with serious diseases of several crops but they are especially widespread in vegetables where they cause loss of yield. These viruses are unique within the family Closteroviridae as they possess bipartite genomes that are separately encapsidated in long filamentous virions. Members of the genus Crinivirus are semi-persistently transmitted by whiteflies belonging to the genera Bemisia and Trialeurodes. The rapid increase and spread of whitefly populations has significantly accelerated the worldwide distribution of Criniviruses and the emergence of new diseases associated with them. This chapter aims to update our knowledge on the molecular biology, epidemiology, diagnostics and management of this important virus group. A special emphasis is given to the description of the specific characteristics of virus species infecting major vegetable crops including tomato, cucurbits, tuber crops, lettuce and bean.
-
Integration of Omics Approaches toward Understanding Whitefly Transmission of Viruses.
Advances in virus research, 2018Co-Authors: William M WintermantelAbstract:Abstract Viruses transmitted by whiteflies are predominantly classified as having either persistent circulative or semipersistent transmission, and the majority of studies have addressed transmission of viruses in the genera Begomovirus (family Geminiviridae) and Crinivirus (family Closteroviridae), respectively. Early studies on vector transmission primarily addressed individual aspects of transmission; however, with the breadth of new technology now available, an increasingly greater number of studies involve coordinated research that is beginning to assemble a more complete picture of how whiteflies and viruses have coevolved to facilitate transmission. In particular the integration of gene expression and metabolomic studies into broader research topics is providing knowledge of changes within the whitefly vector in response to the presence of viruses that would have been impossible to identify previously. Examples include comparative studies on the response of Bemisia tabaci to begomovirus and Crinivirus infection of common host plants, evolution of whitefly endosymbiont relationships, and opportunities to evaluate responses to specific transmission-related events. Integration of metabolomics, as well as the application of electrical penetration graphing, can lead to an ability to monitor the changes that occur in vector insects associated with specific aspects of virus transmission. Through gaining more complete knowledge of the mechanisms behind whitefly transmission of viruses new control strategies will undoubtedly emerge for control of whiteflies and the viruses they transmit.
-
Comparative transcriptome analysis reveals networks of genes activated in the whitefly, Bemisia tabaci when fed on tomato plants infected with Tomato yellow leaf curl virus.
Virology, 2017Co-Authors: Daniel K. Hasegawa, Zhangjun Fei, William M Wintermantel, Yi Zheng, Navneet Kaur, Wenbo Chen, Alvin M. Simmons, Kai-shu LingAbstract:Abstract The whitefly Bemisia tabaci can transmit hundreds of viruses to numerous agricultural crops in the world. Five genera of viruses, including Begomovirus and Crinivirus, are transmitted by B. tabaci. There is little knowledge about the genes involved in virus acquisition and transmission by whiteflies. Using a comparative transcriptomics approach, we evaluated the gene expression profiles of whiteflies (B. tabaci MEAM1) after feeding on tomato infected by a begomovirus, Tomato yellow leaf curl virus (TYLCV), in comparison to a recent study, in which whiteflies were fed on tomato infected by the Crinivirus, Tomato chlorosis virus (ToCV). The data revealed similar temporal trends in gene expression, but large differences in the number of whitefly genes when fed on TYLCV or ToCV-infected tomato. Transcription factors, cathepsins, receptors, and a hemocyanin gene, which is implicated in mediating antiviral immune responses in other insects and possibly virus transmission, were some of the genes identified.
-
Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the Crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes
BMC Genomics, 2017Co-Authors: Navneet Kaur, Daniel K. Hasegawa, Zhangjun Fei, Kai-shu Ling, Yi Zheng, Wenbo Chen, William M WintermantelAbstract:BackgroundWhiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is transmitted by the whitefly (Bemisia tabaci) in a semipersistent manner and infects several globally important agricultural and ornamental crops, including tomato.ResultsTo determine changes in global gene regulation in whiteflies after feeding on tomato plants infected with a Crinivirus (ToCV), comparative transcriptomic analysis was performed using RNA-Seq on whitefly (Bemisia tabaci MEAM1) populations after 24, 48, and 72 h acquisition access periods on either ToCV-infected or uninfected tomatoes. Significant differences in gene expression were detected between whiteflies fed on ToCV-infected tomato and those fed on uninfected tomato among the three feeding time periods: 447 up-regulated and 542 down-regulated at 24 h, 4 up-regulated and 7 down-regulated at 48 h, and 50 up-regulated and 160 down-regulated at 72 h. Analysis revealed differential regulation of genes associated with metabolic pathways, signal transduction, transport and catabolism, receptors, glucose transporters, α-glucosidases, and the uric acid pathway in whiteflies fed on ToCV-infected tomatoes, as well as an abundance of differentially regulated novel orphan genes. Results demonstrate for the first time, a specific and temporally regulated response by the whitefly to feeding on a host plant infected with a semipersistently transmitted virus, and advance the understanding of the whitefly vector-virus interactions that facilitate virus transmission.ConclusionWhitefly transmission of semipersistent viruses is believed to require specific interactions between the virus and its vector that allow binding of virus particles to factors within whitefly mouthparts. Results provide a broader understanding of the potential mechanism of Crinivirus transmission by whitefly, aid in discerning genes or loci in whitefly that influence virus interactions or transmission, and subsequently facilitate development of novel, genetics-based control methods against whitefly and whitefly-transmitted viruses.
Jesús Navas-castillo - One of the best experts on this subject based on the ideXlab platform.
-
Stylet penetration activities of the whitefly Bemisia tabaci associated with inoculation of the Crinivirus Tomato chlorosis virus.
The Journal of general virology, 2017Co-Authors: Nathalie Kristine Prado Maluta, Jesús Navas-castillo, Elisa Garzo, Aranzazu Moreno, Elvira Fiallo-olivé, João Roberto Spotti Lopes, Alberto FereresAbstract:Bemisiatabaci is an important vector of numerous plant viruses, including the emergent semi-persistently transmitted Crinivirus Tomato chlorosis virus (ToCV). Its vector feeding behaviour is complex, with important implications for virus transmission, epidemiology and control. Thus, the objective of this study was to investigate the role of the stylet penetration activities of B. tabaci in the inoculation of ToCV in tomatoes by using the electrical penetration graph (EPG) technique. EPG recordings were classified into six categories depending on the waveforms observed. The results showed that ToCV inoculation is mainly associated with stylet activities in phloem sieve elements (E1 waveform), as there was a significant increase in the rate of transmission when whiteflies performed waveform E1. The precise stylet activities – either salivation or egestion – associated with virion release, presumably from retention sites in the foregut, need further investigation.
-
Phlorizin: a review
2016Co-Authors: Jesús Navas-castillo, Mediterranea La Mayora, Mauricio Montero Astúa, Department Of PlantAbstract:doi: 10.3389/fmicb.2013.00077 A quantum dot-immunofluorescent labeling method to investigate the interactions between a Crinivirus and its whitefly vecto
-
The p22 RNA Silencing Suppressor of the Crinivirus Tomato chlorosis virus is Dispensable for Local Viral Replication but Important for Counteracting an Antiviral RDR6-Mediated Response during Systemic Infection.
Viruses, 2016Co-Authors: Yazmín M. Landeo-ríos, Enrique Moriones, Jesús Navas-castillo, M. Carmen CañizaresAbstract:Among the components of the RNA silencing pathway in plants, RNA-dependent RNA polymerases (RDRs) play fundamental roles in antiviral defence. Here, we demonstrate that the Nicotiana benthamiana RDR6 is involved in defence against the bipartite Crinivirus (genus Crinivirus, family Closteroviridae) Tomato chlorosis virus (ToCV). Additionally, by producing a p22-deficient ToCV infectious mutant clone (ToCVΔp22), we studied the role of this viral suppressor of RNA silencing in viral infection in both wild-type and RDR6-silenced N. benthamiana (NbRDR6i) plants. We demonstrate that p22 is dispensable for the replication of ToCV, where RDR6 appears not to have any effect. Furthermore, the finding that ToCV∆p22 systemic accumulation was impaired in wild-type N. benthamiana but not in NbRDR6i plants suggests a role for p22 in counteracting an RDR6-mediated antiviral response of the plant during systemic infection.
-
Genetic diversity and silencing suppression activity of the p22 protein of Tomato chlorosis virus isolates from tomato and sweet pepper
Virus Genes, 2015Co-Authors: Yazmín M. Landeo-ríos, Enrique Moriones, Jesús Navas-castillo, M. Carmen CañizaresAbstract:As for other bipartite Criniviruses (genus Crinivirus , family Closteroviridae ), the genome of Tomato chlorosis virus encodes an RNA silencing suppressor, the protein p22, in the 3′-proximal region of RNA1. This protein has been reported as having one of the longest lasting local suppressor activities when transiently expressed in Nicotiana benthamiana . Here, we examined the genetic diversity of the p22 gene in ToCV isolates from tomato and sweet pepper. The p22 gene sequences clearly grouped into two separated clades. However, functional analysis of both types of p22 proteins indicated no evident differences in suppressor activity. Our findings provide experimental evidence that the presence of a “strong” silencing suppressor is a conserved feature of ToCV isolates.
-
Multiple suppressors of RNA silencing encoded by both genomic RNAs of the
2015Co-Authors: Carmen M. Cañizares, Jesús Navas-castillo, Enrique MorionesAbstract:Crinivirus, Tomato chlorosis viru
Ioannis E. Tzanetakis - One of the best experts on this subject based on the ideXlab platform.
-
Complete nucleotide sequence of a strawberry isolate of Beet pseudo-yellows virus
2015Co-Authors: Ioannis E. Tzanetakis, Robert Martin R. YAbstract:Abstract. In our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after RT-PCR using primer sets representing multiple regions of Strawberry pallidosis associated virus (SPaV) genome and failed to react with antibodies directed against the recombinant coat protein (CP) of SPaV. DsRNA extracted from this plant showed a similar pattern to that of SPaV indicating that another Crinivirus may infect strawberry. Sequence analysis of multiple cDNA clones corresponding to the heat shock 70 homolog gene (HSP70h) of the unknown virus indicated that it was Beet pseudoyellows virus (BPYV). Analysis of the complete nucleo-tide sequence of BPYV-strawberry revealed that this isolate has several distinct features when compared to Cucumber yellows virus (CuYV), a cucumber strain of BPYV, including an entire ORF not found in CuYV
-
Epidemiology of Criniviruses: an emerging problem in world agriculture
Frontiers in microbiology, 2013Co-Authors: Ioannis E. Tzanetakis, Robert R Martin, William M WintermantelAbstract:The genus Crinivirus includes the whitefly-transmitted members of the family Closteroviridae. Whitefly-transmitted viruses have emerged as a major problem for world agriculture and are responsible for diseases that lead to losses measured in the billions of dollars annually. Criniviruses emerged as a major agricultural threat at the end of the twentieth century with the establishment and naturalization of their whitefly vectors, members of the genera Trialeurodes and Bemisia, in temperate climates around the globe. Several Criniviruses cause significant diseases in single infections whereas others remain asymptomatic and only cause disease when found in mixed infections with other viruses. Characterization of the majority of Criniviruses has been done in the last twenty years and this article provides a detailed review on the epidemiology of this important group of viruses.
-
Population structure of Blackberry yellow vein associated virus, an emerging Crinivirus.
Virus research, 2012Co-Authors: Bindu Poudel, Sead Sabanadzovic, Jozef J. Bujarski, Ioannis E. TzanetakisAbstract:Blackberry yellow vein disease (BYVD), a disorder caused by virus complexes, has become a major threat to fresh market blackberry production in the United States. Blackberry yellow vein associated virus (BYVaV) is the most prevalent virus in the BYVD complexes; detected in about 50% of samples exhibiting typical disease symptoms. Thirty-four virus isolates infecting wild and cultivated blackberries were collected from several areas with high BYVD incidence. Sequence variability and virus evolution predictions were calculated for four genomic regions coding for six proteins and accounting for about 30% of the virus genome. Nucleotide diversity ranged between 7 and 12%, and all proteins studied were under negative selection. Several isolates were identified as potential recombinants suggesting that recombination might be a driving force behind BYVaV evolution.
-
Diodia vein chlorosis virus is a group-1 Crinivirus
Archives of Virology, 2011Co-Authors: Ioannis E. Tzanetakis, William M Wintermantel, Bindu Poudel, Jing ZhouAbstract:Members of the family Closteroviridae have emerged as a major problem in agricultural crops in the past two decades. Diodia vein chlorosis virus (DVCV) is an understudied whitefly-transmitted closterovirus. Given the presence of the primary host for the virus in major agricultural production areas in the United States, we characterized the virus at the molecular level, demonstrating that it belongs in the genus Crinivirus , developed detection protocols, evaluated its host range among hosts known to harbor viruses closely related to DVCV, and confirmed transmission by a second whitefly species, Trialeurodes vaporariorum .
-
further complexity of the genus Crinivirus revealed by the complete genome sequence of lettuce chlorosis virus lcv and the similar temporal accumulation of lcv genomic rnas 1 and 2
Virology, 2009Co-Authors: Nida M Salem, Ioannis E. Tzanetakis, Angel Y S Chen, Chawin MongkolsiriwattanaAbstract:The sequence of Lettuce chlorosis virus (LCV) (genus Crinivirus) was determined and found to contain unique open reading frames (ORFs) and ORFs similar to those of other Criniviruses, as well as 3' non-coding regions that shared a high degree of identity. Northern blot analysis of RNA extracted from LCV-infected plants identified subgenomic RNAs corresponding to six prominent internal ORFs and detected several novel LCV-single stranded RNA species. Virus replication in tobacco protoplasts was investigated and results indicated that LCV replication proceeded with novel Crinivirus RNA accumulation kinetics, wherein viral genomic RNAs exhibited a temporally similar expression pattern early in the infection. This was noticeably distinct from the asynchronous RNA accumulation pattern previously observed for Lettuce infectious yellows virus (LIYV), the type member of the genus, suggesting that replication of the two viruses likely operate via dissimilar mechanisms.
Kai-shu Ling - One of the best experts on this subject based on the ideXlab platform.
-
Comparative transcriptome analysis reveals networks of genes activated in the whitefly, Bemisia tabaci when fed on tomato plants infected with Tomato yellow leaf curl virus.
Virology, 2017Co-Authors: Daniel K. Hasegawa, Zhangjun Fei, William M Wintermantel, Yi Zheng, Navneet Kaur, Wenbo Chen, Alvin M. Simmons, Kai-shu LingAbstract:Abstract The whitefly Bemisia tabaci can transmit hundreds of viruses to numerous agricultural crops in the world. Five genera of viruses, including Begomovirus and Crinivirus, are transmitted by B. tabaci. There is little knowledge about the genes involved in virus acquisition and transmission by whiteflies. Using a comparative transcriptomics approach, we evaluated the gene expression profiles of whiteflies (B. tabaci MEAM1) after feeding on tomato infected by a begomovirus, Tomato yellow leaf curl virus (TYLCV), in comparison to a recent study, in which whiteflies were fed on tomato infected by the Crinivirus, Tomato chlorosis virus (ToCV). The data revealed similar temporal trends in gene expression, but large differences in the number of whitefly genes when fed on TYLCV or ToCV-infected tomato. Transcription factors, cathepsins, receptors, and a hemocyanin gene, which is implicated in mediating antiviral immune responses in other insects and possibly virus transmission, were some of the genes identified.
-
Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the Crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes
BMC Genomics, 2017Co-Authors: Navneet Kaur, Daniel K. Hasegawa, Zhangjun Fei, Kai-shu Ling, Yi Zheng, Wenbo Chen, William M WintermantelAbstract:BackgroundWhiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is transmitted by the whitefly (Bemisia tabaci) in a semipersistent manner and infects several globally important agricultural and ornamental crops, including tomato.ResultsTo determine changes in global gene regulation in whiteflies after feeding on tomato plants infected with a Crinivirus (ToCV), comparative transcriptomic analysis was performed using RNA-Seq on whitefly (Bemisia tabaci MEAM1) populations after 24, 48, and 72 h acquisition access periods on either ToCV-infected or uninfected tomatoes. Significant differences in gene expression were detected between whiteflies fed on ToCV-infected tomato and those fed on uninfected tomato among the three feeding time periods: 447 up-regulated and 542 down-regulated at 24 h, 4 up-regulated and 7 down-regulated at 48 h, and 50 up-regulated and 160 down-regulated at 72 h. Analysis revealed differential regulation of genes associated with metabolic pathways, signal transduction, transport and catabolism, receptors, glucose transporters, α-glucosidases, and the uric acid pathway in whiteflies fed on ToCV-infected tomatoes, as well as an abundance of differentially regulated novel orphan genes. Results demonstrate for the first time, a specific and temporally regulated response by the whitefly to feeding on a host plant infected with a semipersistently transmitted virus, and advance the understanding of the whitefly vector-virus interactions that facilitate virus transmission.ConclusionWhitefly transmission of semipersistent viruses is believed to require specific interactions between the virus and its vector that allow binding of virus particles to factors within whitefly mouthparts. Results provide a broader understanding of the potential mechanism of Crinivirus transmission by whitefly, aid in discerning genes or loci in whitefly that influence virus interactions or transmission, and subsequently facilitate development of novel, genetics-based control methods against whitefly and whitefly-transmitted viruses.
-
Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the Crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes.
BMC genomics, 2017Co-Authors: Navneet Kaur, Daniel K. Hasegawa, Zhangjun Fei, Kai-shu Ling, Yi Zheng, Wenbo Chen, William M WintermantelAbstract:Whiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is transmitted by the whitefly (Bemisia tabaci) in a semipersistent manner and infects several globally important agricultural and ornamental crops, including tomato. To determine changes in global gene regulation in whiteflies after feeding on tomato plants infected with a Crinivirus (ToCV), comparative transcriptomic analysis was performed using RNA-Seq on whitefly (Bemisia tabaci MEAM1) populations after 24, 48, and 72 h acquisition access periods on either ToCV-infected or uninfected tomatoes. Significant differences in gene expression were detected between whiteflies fed on ToCV-infected tomato and those fed on uninfected tomato among the three feeding time periods: 447 up-regulated and 542 down-regulated at 24 h, 4 up-regulated and 7 down-regulated at 48 h, and 50 up-regulated and 160 down-regulated at 72 h. Analysis revealed differential regulation of genes associated with metabolic pathways, signal transduction, transport and catabolism, receptors, glucose transporters, α-glucosidases, and the uric acid pathway in whiteflies fed on ToCV-infected tomatoes, as well as an abundance of differentially regulated novel orphan genes. Results demonstrate for the first time, a specific and temporally regulated response by the whitefly to feeding on a host plant infected with a semipersistently transmitted virus, and advance the understanding of the whitefly vector-virus interactions that facilitate virus transmission. Whitefly transmission of semipersistent viruses is believed to require specific interactions between the virus and its vector that allow binding of virus particles to factors within whitefly mouthparts. Results provide a broader understanding of the potential mechanism of Crinivirus transmission by whitefly, aid in discerning genes or loci in whitefly that influence virus interactions or transmission, and subsequently facilitate development of novel, genetics-based control methods against whitefly and whitefly-transmitted viruses.
Navneet Kaur - One of the best experts on this subject based on the ideXlab platform.
-
Comparative transcriptome analysis reveals networks of genes activated in the whitefly, Bemisia tabaci when fed on tomato plants infected with Tomato yellow leaf curl virus.
Virology, 2017Co-Authors: Daniel K. Hasegawa, Zhangjun Fei, William M Wintermantel, Yi Zheng, Navneet Kaur, Wenbo Chen, Alvin M. Simmons, Kai-shu LingAbstract:Abstract The whitefly Bemisia tabaci can transmit hundreds of viruses to numerous agricultural crops in the world. Five genera of viruses, including Begomovirus and Crinivirus, are transmitted by B. tabaci. There is little knowledge about the genes involved in virus acquisition and transmission by whiteflies. Using a comparative transcriptomics approach, we evaluated the gene expression profiles of whiteflies (B. tabaci MEAM1) after feeding on tomato infected by a begomovirus, Tomato yellow leaf curl virus (TYLCV), in comparison to a recent study, in which whiteflies were fed on tomato infected by the Crinivirus, Tomato chlorosis virus (ToCV). The data revealed similar temporal trends in gene expression, but large differences in the number of whitefly genes when fed on TYLCV or ToCV-infected tomato. Transcription factors, cathepsins, receptors, and a hemocyanin gene, which is implicated in mediating antiviral immune responses in other insects and possibly virus transmission, were some of the genes identified.
-
Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the Crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes
BMC Genomics, 2017Co-Authors: Navneet Kaur, Daniel K. Hasegawa, Zhangjun Fei, Kai-shu Ling, Yi Zheng, Wenbo Chen, William M WintermantelAbstract:BackgroundWhiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is transmitted by the whitefly (Bemisia tabaci) in a semipersistent manner and infects several globally important agricultural and ornamental crops, including tomato.ResultsTo determine changes in global gene regulation in whiteflies after feeding on tomato plants infected with a Crinivirus (ToCV), comparative transcriptomic analysis was performed using RNA-Seq on whitefly (Bemisia tabaci MEAM1) populations after 24, 48, and 72 h acquisition access periods on either ToCV-infected or uninfected tomatoes. Significant differences in gene expression were detected between whiteflies fed on ToCV-infected tomato and those fed on uninfected tomato among the three feeding time periods: 447 up-regulated and 542 down-regulated at 24 h, 4 up-regulated and 7 down-regulated at 48 h, and 50 up-regulated and 160 down-regulated at 72 h. Analysis revealed differential regulation of genes associated with metabolic pathways, signal transduction, transport and catabolism, receptors, glucose transporters, α-glucosidases, and the uric acid pathway in whiteflies fed on ToCV-infected tomatoes, as well as an abundance of differentially regulated novel orphan genes. Results demonstrate for the first time, a specific and temporally regulated response by the whitefly to feeding on a host plant infected with a semipersistently transmitted virus, and advance the understanding of the whitefly vector-virus interactions that facilitate virus transmission.ConclusionWhitefly transmission of semipersistent viruses is believed to require specific interactions between the virus and its vector that allow binding of virus particles to factors within whitefly mouthparts. Results provide a broader understanding of the potential mechanism of Crinivirus transmission by whitefly, aid in discerning genes or loci in whitefly that influence virus interactions or transmission, and subsequently facilitate development of novel, genetics-based control methods against whitefly and whitefly-transmitted viruses.
-
Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the Crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes.
BMC genomics, 2017Co-Authors: Navneet Kaur, Daniel K. Hasegawa, Zhangjun Fei, Kai-shu Ling, Yi Zheng, Wenbo Chen, William M WintermantelAbstract:Whiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is transmitted by the whitefly (Bemisia tabaci) in a semipersistent manner and infects several globally important agricultural and ornamental crops, including tomato. To determine changes in global gene regulation in whiteflies after feeding on tomato plants infected with a Crinivirus (ToCV), comparative transcriptomic analysis was performed using RNA-Seq on whitefly (Bemisia tabaci MEAM1) populations after 24, 48, and 72 h acquisition access periods on either ToCV-infected or uninfected tomatoes. Significant differences in gene expression were detected between whiteflies fed on ToCV-infected tomato and those fed on uninfected tomato among the three feeding time periods: 447 up-regulated and 542 down-regulated at 24 h, 4 up-regulated and 7 down-regulated at 48 h, and 50 up-regulated and 160 down-regulated at 72 h. Analysis revealed differential regulation of genes associated with metabolic pathways, signal transduction, transport and catabolism, receptors, glucose transporters, α-glucosidases, and the uric acid pathway in whiteflies fed on ToCV-infected tomatoes, as well as an abundance of differentially regulated novel orphan genes. Results demonstrate for the first time, a specific and temporally regulated response by the whitefly to feeding on a host plant infected with a semipersistently transmitted virus, and advance the understanding of the whitefly vector-virus interactions that facilitate virus transmission. Whitefly transmission of semipersistent viruses is believed to require specific interactions between the virus and its vector that allow binding of virus particles to factors within whitefly mouthparts. Results provide a broader understanding of the potential mechanism of Crinivirus transmission by whitefly, aid in discerning genes or loci in whitefly that influence virus interactions or transmission, and subsequently facilitate development of novel, genetics-based control methods against whitefly and whitefly-transmitted viruses.