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Masayoshi Yamaguchi - One of the best experts on this subject based on the ideXlab platform.
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Role of carotenoid β-Cryptoxanthin in bone homeostasis
Journal of Biomedical Science, 2012Co-Authors: Masayoshi YamaguchiAbstract:Bone homeostasis is maintained through a balance between osteoblastic bone formation and osteoclastic bone resorption. Aging induces bone loss due to decreased osteoblastic bone formation and increased osteoclastic bone resorption. Osteoporosis with its accompanying decrease in bone mass is widely recognized as a major public health problem. Nutritional factors may play a role in the prevention of bone loss with aging. Among various carotenoids (carotene and xanthophylls including beta (β)-Cryptoxanthin, lutein, lycopene, β-carotene, astaxanthin, and rutin), β-Cryptoxanthin, which is abundant in Satsuma mandarin orange ( Citrus unshiu MARC.), has been found to have a stimulatory effect on bone calcification in vitro . β-Cryptoxanthin has stimulatory effects on osteoblastic bone formation and inhibitory effects on osteoclastic bone resorption in vitro , thereby increasing bone mass. β-Cryptoxanthin has an effect on the gene expression of various proteins that are related osteoblastic bone formation and osteoclastic bone resororption in vitro . The intake of β-Cryptoxanthin may have a preventive effect on bone loss in animal models for osteoporosis and in healthy human or postmenopausal women. Epidemiological studies suggest a potential role of β-Cryptoxanthin as a sustainable nutritional approach to improving bone health of human subjects. β-Cryptoxanthin may be an osteogenic factor in preventing osteoporosis in human subjects.
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The bone anabolic carotenoid β-Cryptoxanthin enhances transforming growth factor-β1-induced SMAD activation in MC3T3 preosteoblasts
International journal of molecular medicine, 2009Co-Authors: Masayoshi Yamaguchi, M. Neale WeitzmannAbstract:The xanthophyll beta-Cryptoxanthin is a member of the carotenoid family of plant-derived pigments endowed with anti-osteoporotic properties in vivo. beta-Cryptoxanthin was demonstrated to stimulate osteoblastic bone formation and simultaneously repress osteoclastic bone resorption in vitro. However, the mechanisms of action remain to be elucidated. The SMAD signal transduction pathway is established to play a critical role in osteoblast lineage commitment and differentiation. In this study we used transient transfection assays of a SMAD luciferase reporter to investigate whether beta-Cryptoxanthin regulates SMAD activation in MC3T3 pre-osteoblastic cells. Beta-Cryptoxanthin did not stimulate basal SMAD activity but amplified transforming growth factor (TGF)-beta1-induced SMAD activation. Interestingly, beta-Cryptoxanthin did not affect bone morphogenetic protein-2 (BMP-2)-induced SMAD activation in osteoblastic cells, suggesting specificity of action on the TGF-beta1 pathway. This study suggests that the carotenoid beta-Cryptoxanthin may promote osteoblast differentiation and activity by amplifying TGF-beta1-induced lineage commitment of osteoblast precursors.
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β-Cryptoxanthin and Bone Metabolism: The Preventive Role in Osteoporosis
JOURNAL OF HEALTH SCIENCE, 2008Co-Authors: Masayoshi YamaguchiAbstract:Bone loss with aging induces osteoporosis. The most dramatic expression of the disease is represented by fractures of the proximal femur. Pharmacologic and nutritional factors may play a role in the prevention of bone loss with aging. β-Cryptoxanthin, a kind of carotenoid, is abundant in Satsuma mandarin orange (Citrus unshiu MARC.). Amoung various carotenoids including β-Cryptoxanthin, lutein, lycopene, β-carotene, astaxanthin, and rutin, β-Cryptoxanthin has been found to have a unique anabolic effect on bone calcification in vitro. Hesperidin, which is contained in Satsuma mandarin orange, did not have an anabolic effect on bone calcification in vitro. β-Cryptoxanthin has stimulatory effects on osteoblastic bone formation and inhibitory effects on osteoclastic bone resorption in vitro, thereby increasing bone mass. β-Cryptoxanthin has an effect on the gene expression of various proteins which are related to osteoblastic bone formation and mineralizationin vitro. β-Cryptoxanthin has inhibitory effects on enzyme activity which is related to osteoclastic bone resororption, and the carotenoid induces apoptosis of mature osteoclastic cells in vitro. Oral administration of β-Cryptoxanthin has been shown to have the anabolic effects on bone components in young and aged rats, and the administration has the preventive effects on bone loss in streptozotocin-diabetic rats and ovariectomized rats in vivo. Moreover, the intake of β-Cryptoxanthin-reinforced juice for longer periods has been shown to have both stimulatory effects on bone formation and inhibitory effects on bone resorption in healthy human or postmenopausal women in evaluating with serum biochemical markers of bone metabolism in vivo. Thus the intake of dietary β-Cryptoxanthin may have a preventive effect on osteoporosis due to stimulating bone formation and due to inhibiting bone resorption. Moreover, epidemiological studies suggest the potential role of β-Cryptoxanthin as a sustainable nutritional approach to improving bone health of human subjects. β-Cryptoxanthin is an important food factor in maintaining bone healthy and in preventing osteoporosis.
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Osteoporosis Prevention by β-Cryptoxanthin
2008Co-Authors: Masayoshi Yamaguchi, Satoshi UchiyamaAbstract:Pharmacologic and nutritional factors may play a role in the prevention of osteoporosis with aging. P-Cryptoxanthin, a carotenoid, is abundant in Satsuma mandarin orange. Among various carotenoid including p-Cryptoxanthin, lutein, lycopene, β-carotene, astaxanthin, and rutin, P-Cryptoxanthin has been found to have a unique anabolic effect on bone calcification. P-Cryptoxanthin has stimulatory effects on osteoblastic bone formation and inhibitory effects on osteoclastic bone resorption in vitro. β-Cryptoxanthin has an effect on the gene expression of various proteins which are related to osteoblastic bone formation and osteoclastic bone resorption. Oral administration of P-Cryptoxanthin has been shown to have the anabolic effect on bone components in young and aged rats, the preventive effect on bone loss in streptozotocin-diabetic rats and ovariectomy-induced bone loss. Moreover, the intake of β-Cryptoxanthin-reinforced juice for longer periods has been shown to have stimulatory effects on bone formation and inhibitory effects on bone resorption in healthy human and postmenopausal women as estimated based on serum biochemical markers of bone metabolism in vivo. The intake of dietary β-Cryptoxanthin may have a preventive effect on osteoporosis.
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effect of β crytoxanthin on circulating bone metabolic markers intake of juice citrus unshiu supplemented with β Cryptoxanthin has an effect in menopausal women
Journal of Health Science, 2006Co-Authors: Masayoshi Yamaguchi, Aki Igarashi, Satoshi Uchiyama, Seiichi Morita, Kuniaki Sugawara, Takashi Sumida, Hiroshi Ogawa, Masahito Nishitani, Yoshitaka KajimotoAbstract:The effects of prolonged intake of juice prepared from Satsuma mandarin (Citrus unshiu MARC.) containing β-Cryptoxanthin on circulating biochemical markers of bone metabolism in subjects, including menopausal woman, were investigated. Ninety volunteers, aged 27-65 years (19 men and 71 women), were enrolled in this study. The 71 females included 35 premenopausal women (ages, 27-50 years) and 36 postmenopausal women (ages, 46-65 years). Volunteers were divided into four groups; placebo juice without β-cyptoxanthin (5 men and 19 women), juice containing β-cyptoxanthin at 1.5 mg/200 ml of juice/day (4 men and 17 women), 3.0 mg/day (5 men and 17 women), and 6.0 mg/day (5 men and 18 women). Placebo or juice (200 ml) was ingested once a day for 28 or 56 days. Serum β-Cryptoxanthin concentrations were significantly increased after the intake of juice containing β-Cryptoxanthin (1.5, 3.0, or 6.0 mg/day) for 28 or 56 days, and the increases were dose-dependent. Bone-specific alkaline phosphatase and γ-carboxylated osteocalcin are serum bone markers of bone formation, and bone tartrate-resistant acid phosphatase (TRACP) and N-telopeptides of type I collagen are markers of bone resorption. Bone-specific alkaline phosphatase activity was significantly increased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 56 days as compared with the value obtained before intake. γ-Carboxylated osteocalcin concentration was significantly increased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 28 or 56 days as compared with the value obtained before intake or after the intake of placebo juice. Serum TRACP activity and type I collagen N-telopeptide concentration were significantly decreased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 28 or 56 days as compared with the value obtained before intake or after intake of placebo juice, and significant decreases were also seen after the intake of 1.5 mg/day β-Cryptoxanthin as compared with the value obtained before intake. In menopausal women, bone-specific alkaline phosphatase activity and γ-carboxylated osteocalcin concentration were significantly increased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 56 days as compared with the value obtained after placebo intake. Also, this intake caused a significant decrease in bone TRACP activity. Meanwhile, serum calcium, inorganic phosphorous, and parathyroid hormone (intact) were not changed after the intake of β-Cryptoxanthin-containing juice for 28 or 56 days. This study demonstrates that the prolonged intake of juice fortified with β-Cryptoxanthin has stimulatory effects on bone formation and inhibitory effects on bone resorption in humans, and that the intake has an effect in menopausal women.
Xiang-dong Wang - One of the best experts on this subject based on the ideXlab platform.
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Abstract 832: Effects of beta-Cryptoxanthin on cigarette smoke-induced lung oxidative damage, inflammation and activation of NF-kappa B and AP-1
Prevention Research, 2011Co-Authors: Chun Liu, Roderick T. Bronson, Robert M. Russell, Xiang-dong WangAbstract:Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL In epidemiologic studies, high intake of beta-Cryptoxanthin has been associated with a decreased risk of lung cancer, particularly among current smokers. However, data are not available from well-controlled animal studies to examine the effects of beta-Cryptoxanthin on cigarette smoke-induced lung lesions, and the biological mechanisms by which beta-Cryptoxanthin might affect lung carcinogenesis. We evaluated the effects of beta-Cryptoxanthin supplementation on cigarette smoke-induced squamous metaplasia, inflammation, and changes in protein levels of pro-inflammatory cytokine [tumor necrosis factor alpha (TNF-alpha)] and transcription factors [nuclear factor kappa B (NF-kappa B) and activator protein-1 (AP-1)], as well as on smoke-induced oxidative DNA damage [8-hydroxy-2′-deoxyguanosine (8-OHdG)] in the lung tissue of ferrets. Thirty six male ferrets were assigned to cigarette smoke exposure or to no exposure and to no dose, low-dose, or high-dose beta-Cryptoxanthin supplementation (2 × 3 factorial design) for 3 months. Beta-Cryptoxanthin supplementation dose-dependently increased plasma and lung beta-Cryptoxanthin levels in ferrets, whereas cigarette smoke exposure lowered plasma and lung beta-Cryptoxanthin levels. Beta-Cryptoxanthin at both doses significantly decreased smoke-induced lung squamous metaplasia and inflammation. Beta-Cryptoxanthin also substantially reduced smoke-elevated TNF-alpha levels in alveolar, bronchial, bronchiolar and airway serous/mucous gland epithelial cells and in lung macrophages. Moreover, beta-Cryptoxanthin decreased smoke-induced activation of NF-kappa B and expression of AP-1 and 8-OHdG. The beneficial effects of beta-Cryptoxanthin were stronger for high-dose beta-Cryptoxanthin than for low-dose beta-Cryptoxanthin. Data from this study indicate that beta-Cryptoxanthin provides a beneficial effect against cigarette smoke-induced inflammation, oxidative DNA damage and squamous metaplasia in the lungs. Beta-Cryptoxanthin may prevent lung carcinogenesis through its inhibitive effects on lung inflammation and oxidative DNA damage. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 832. doi:10.1158/1538-7445.AM2011-832
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β-Cryptoxanthin supplementation prevents cigarette smoke-induced lung inflammation, oxidative damage and squamous metaplasia in ferrets
Cancer prevention research (Philadelphia Pa.), 2011Co-Authors: Chun Liu, Roderick T. Bronson, Robert M. Russell, Xiang-dong WangAbstract:In epidemiologic studies, high intake of β-Cryptoxanthin has been associated with a decreased risk of lung cancer, particularly among current smokers. However, data are not available from well-controlled animal studies to examine the effects of β-Cryptoxanthin on cigarette smoke-induced lung lesions, and the biological mechanisms by which β-Cryptoxanthin might affect lung carcinogenesis. We evaluated the effects of β-Cryptoxanthin supplementation on cigarette smoke-induced squamous metaplasia, inflammation, and changes in protein levels of proinflammatory cytokine [tumor necrosis factor alpha (TNFα)] and transcription factors [nuclear factor kappa B (NF-κB) and activator protein-1 (AP-1)], as well as on smoke-induced oxidative DNA damage [8-hydroxy-2'-deoxyguanosine (8-OHdG)] in the lung tissue of ferrets. Thirty-six male ferrets were assigned to cigarette smoke exposure or no exposure and to low-dose, or high-dose β-Cryptoxanthin, or no dose (2 × 3 factorial design) for 3 months. β-Cryptoxanthin supplementation dose-dependently increased plasma and lung β-Cryptoxanthin levels in ferrets, whereas cigarette smoke exposure lowered plasma and lung β-Cryptoxanthin levels. β-Cryptoxanthin at both doses significantly decreased smoke-induced lung squamous metaplasia and inflammation. β-Cryptoxanthin also substantially reduced smoke-elevated TNFα levels in alveolar, bronchial, bronchiolar, and bronchial serous/mucous gland epithelial cells and in lung macrophages. Moreover, β-Cryptoxanthin decreased smoke-induced activation of NF-κB, expression of AP-1 and levels of 8-OHdG. The beneficial effects of β-Cryptoxanthin were stronger for high-dose β-Cryptoxanthin than for low-dose β-Cryptoxanthin. Data from this study indicate that β-Cryptoxanthin provides a beneficial effect against cigarette smoke-induced inflammation, oxidative DNA damage and squamous metaplasia in the lungs.
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Abstract LB-430: Chemopreventive effect of beta-Cryptoxanthin on smoke-induced lung inflammation and lesions in ferrets
Prevention Research, 2010Co-Authors: Chun Liu, Roderick T. Bronson, Xiang-dong WangAbstract:Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC Background: High intake of beta-Cryptoxanthin has been associated with a decreased risk of lung cancer, particularly among current smokers in epidemiological studies. However, there are no available data from well-controlled animal studies to examine the effect of beta-Cryptoxanthin on smoke-induced lung lesions and potential biological mechanisms by which beta-Cryptoxanthin affects lung carcinogenesis. Using a ferret animal model, we evaluated the effect of beta-Cryptoxanthin supplementation in both low-and high-dose (7.5 mcg/kg body weight per day vs. 37.5 mcg/kg body weight per day in ferrets which are equivalent to 104 mcg/day vs. 520 mcg/day, respectively, in the humans) on the cigarette smoke-induced pro-inflammatory cytokine TNF alpha, inflammation and squamous metaplasia in the lung. Methods: Thirty six male ferrets were assigned to one of six groups (n = 6 in each group) for 9 weeks as follows: 1) control; 2) cigarette smoke exposed; 3) low-dose beta-Cryptoxanthin (7.5 mcg/kg body weight per day); 4) high-dose beta-Cryptoxanthin (37.5 mcg/kg body weight per day); 5) cigarette smoke-exposed plus low-dose beta-Cryptoxanthin; and 6) cigarette smoke-exposed plus high-dose beta-Cryptoxanthin. Lung TNF alpha was measured by immunohistochemical staining. Lung inflammation and squamous metaplasia were quantified by histopathological evaluations. Results: Beta-Cryptoxanthin supplementation dose-dependently increased plasma and lung beta-Cryptoxanthin levels in ferrets. Cigarette smoke exposure lowered plasma and lung beta-Cryptoxanthin levels. Treatment with beta-Cryptoxanthin substantially reduced the cigarette smoke-elevated TNF alpha levels in bronchial/bronchiolar epithelial cells, alveolar epithelial cells, airway serous/mucous glands and lung macrophages. Beta-Cryptoxanthin at both doses also significantly decreased the cigarette smoke-induced lung inflammation. In addition, both doses of beta-Cryptoxanthin significantly lowered the incidence of lung squamous metaplasia induced by cigarette smoke exposure. There were no detectable squamous metaplasia in the groups treated with beta-Cryptoxanthin alone and in the control group. Conclusions: Beta-Cryptoxanthin may provide a beneficial effect against the smoke-induced lung inflammation and lung lesions. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-430.
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β Cryptoxanthin suppresses the growth of immortalized human bronchial epithelial cells and non small cell lung cancer cells and up regulates retinoic acid receptor β expression
International Journal of Cancer, 2006Co-Authors: Fuzhi Lian, Robert M. Russell, Xiang-dong WangAbstract:Recent findings of an inverse association between beta-Cryptoxanthin and lung cancer risk in several observational epidemiologic studies suggest that beta-Cryptoxanthin could potentially act as a chemopreventive agent against lung cancer. However, the biological activity of beta-Cryptoxanthin and molecular mechanism(s) by which beta-Cryptoxanthin affects lung tumourigenesis have not been studied. In the present study, we found that beta-Cryptoxanthin inhibited the growth of A549 cells, a non-small-cell lung cancer cell line and BEAS-2B cells, an immortalized human bronchial epithelial cell line in a dose-dependent manner. beta-Cryptoxanthin suppressed the protein levels of cyclin D1 and cyclin E, up-regulated the cell cycle inhibitor p21, increased the number of lung cancer cells in the G1/G0 phase and decreased those in the S phase of the cell cycle. Consistent with inhibition of the lung cancer cell growth, beta-Cryptoxanthin induced the mRNA levels of retinoic acid receptor beta (RARbeta) in BEAS-2B cells, although this effect was less pronounced in A549 cells. Furthermore, beta-Cryptoxanthin transactivated RAR-mediated transcription activity of the retinoic acid response element. These findings suggest a mechanism of anti-proliferative action of beta-Cryptoxanthin and indicate that beta-Cryptoxanthin may be a promising chemopreventive agent against lung cancer.
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β‐Cryptoxanthin suppresses the growth of immortalized human bronchial epithelial cells and non‐small‐cell lung cancer cells and up‐regulates retinoic acid receptor β expression
International journal of cancer, 2006Co-Authors: Fuzhi Lian, Robert M. Russell, Xiang-dong WangAbstract:Recent findings of an inverse association between beta-Cryptoxanthin and lung cancer risk in several observational epidemiologic studies suggest that beta-Cryptoxanthin could potentially act as a chemopreventive agent against lung cancer. However, the biological activity of beta-Cryptoxanthin and molecular mechanism(s) by which beta-Cryptoxanthin affects lung tumourigenesis have not been studied. In the present study, we found that beta-Cryptoxanthin inhibited the growth of A549 cells, a non-small-cell lung cancer cell line and BEAS-2B cells, an immortalized human bronchial epithelial cell line in a dose-dependent manner. beta-Cryptoxanthin suppressed the protein levels of cyclin D1 and cyclin E, up-regulated the cell cycle inhibitor p21, increased the number of lung cancer cells in the G1/G0 phase and decreased those in the S phase of the cell cycle. Consistent with inhibition of the lung cancer cell growth, beta-Cryptoxanthin induced the mRNA levels of retinoic acid receptor beta (RARbeta) in BEAS-2B cells, although this effect was less pronounced in A549 cells. Furthermore, beta-Cryptoxanthin transactivated RAR-mediated transcription activity of the retinoic acid response element. These findings suggest a mechanism of anti-proliferative action of beta-Cryptoxanthin and indicate that beta-Cryptoxanthin may be a promising chemopreventive agent against lung cancer.
Minoru Sugiura - One of the best experts on this subject based on the ideXlab platform.
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β-Cryptoxanthin Induces UCP-1 Expression via a RAR Pathway in Adipose Tissue
Journal of agricultural and food chemistry, 2019Co-Authors: Hideyuki Hara, Minoru Sugiura, Haruya Takahashi, Shinsuke Mohri, Hiroki Murakami, Satoko Kawarasaki, Mari Iwase, Nobuyuki Takahashi, Tsuyoshi Goto, Teruo KawadaAbstract:While β-Cryptoxanthin is hypothesized to have a preventive effect on lifestyle-related diseases, its underlying mechanisms are unknown. We investigated the effect of β-Cryptoxanthin on energy metabolism in adipose tissues and its underlying mechanism. C57BL/6J mice were fed a high-fat diet (60% kcal fat) containing 0 or 0.05% β-Cryptoxanthin for 12 weeks. β-Cryptoxanthin treatment was found to reduce body fat gain and plasma glucose level, while increasing energy expenditure. The expression of uncoupling protein (UCP) 1 was elevated in adipose tissues in the treatment group. Furthermore, the in vivo assays showed that the Ucp1 mRNA expression was higher in the β-Cryptoxanthin treatment group, an effect that disappeared upon cotreatment with a retinoic acid receptor (RAR) antagonist. In conclusion, we report that β-Cryptoxanthin reduces body fat and body weight gain and that β-Cryptoxanthin increases the expression of UCP1 via the RAR pathway.
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Health Effects of β-Cryptoxanthin and β-Cryptoxanthin-Enriched Satsuma Mandarin Juice
Nutrients in Beverages, 2019Co-Authors: Mieko Nakamura, Minoru SugiuraAbstract:Abstract Carotenoids are a widely distributed group of naturally occurring pigments, usually yellow, red, or orange in color. Of the six major carotenoids found in human serum, little is known on the physiological functions of β-Cryptoxanthin compared with those of β-carotene or lycopene. In Japan, Satsuma mandarin (Citrus unshiu Marc.), which contains high concentrations of β-Cryptoxanthin, is locally grown and is the most widely consumed domestic citrus fruit. First, characteristics of β-Cryptoxanthin, such as absorption, storage, and distribution are outlined. Next, possible association between β-Cryptoxanthin and lifestyle-related diseases, such as osteoporosis, diabetes, liver dysfunction, arteriosclerosis, and metabolic syndrome were reviewed from the results of experimental and epidemiological observational studies. Finally, β-Cryptoxanthin-enriched Satsuma mandarin juice was introduced, and evidence from randomized controlled trials on the effects of β-Cryptoxanthin-enriched Satsuma mandarin juice supplementation on liver function and cardiovascular risk were summarized.
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β-Cryptoxanthin exerts greater cardioprotective effects on cardiac ischemia-reperfusion injury than astaxanthin by attenuating mitochondrial dysfunction in mice.
Molecular nutrition & food research, 2017Co-Authors: Wanpitak Pongkan, Shuichi Kaneko, Osamu Takatori, Naoto Nagata, Siriporn C. Chattipakorn, Soichiro Usui, Masayuki Takamura, Minoru SugiuraAbstract:cope β-Cryptoxanthin and astaxanthin are antioxidant carotenoid pigments that inhibit lipid peroxidation as potently as vitamin E. We hypothesized that acute treatment with β-Cryptoxanthin and astaxanthin causes similar reductions in the sizes of cardiac infarcts caused by ischemia-reperfusion (I/R) injury by attenuating oxidative stress and cardiac mitochondrial dysfunction. Methods and results C57BL/6 mice (n = 36) were randomized to receive vehicle, β-Cryptoxanthin, astaxanthin or vitamin E at 50 mg/kg by gavage feeding prior to I/R injury. Cardiac I/R was induced by left anterior descending coronary artery ligation followed by reperfusion. All treatments significantly reduced infarct sizes by 36–57%, attenuated apoptosis and also attenuated cardiac mitochondrial dysfunction in the treated groups compared to the control group. Although astaxanthin and vitamin E exhibited similar efficacy with respect to cardioprotection, β-Cryptoxanthin exhibited greater efficacy than its counterparts, as it reduced infarct sizes by 60%. β-Cryptoxanthin was more effective than astaxanthin and vitamin E because it reduced cardiac mitochondrial swelling, mitochondrial depolarization, the Bax/Bcl-2 ratio and plasma and cardiac TBARS levels more significantly than its counterparts. Conclusion Acute β-Cryptoxanthin treatment exhibits greater cardioprotective efficacy against I/R injury than astaxanthin and vitamin E by reducing infarct sizes and attenuating apoptosis, oxidative stress and mitochondrial dysfunction. This article is protected by copyright. All rights reserved
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Daily oral intake of β-Cryptoxanthin ameliorates neuropathic pain.
Bioscience biotechnology and biochemistry, 2017Co-Authors: Gyujin Park, Minoru Sugiura, Tetsuhiro Horie, Kazuya Fukasawa, Kakeru Ozaki, Yuki Onishi, Takashi Kanayama, Takashi Iezaki, Maika Okamoto, Eiichi HinoiAbstract:β-Cryptoxanthin, a xanthophyll carotenoid, exerts preventive effects on various lifestyle-related diseases. Here, we found that daily oral administration of β-Cryptoxanthin significantly ameliorated the development of tactile allodynia following spinal nerve injury but was ineffective in mechanical allodynia in an inflammatory pain model in mice. Our results suggest that β-Cryptoxanthin supplementation would be beneficial for the prophylaxis of neuropathic pain.
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Amelioration of the Development of Osteoarthritis by Daily Intake of β-Cryptoxanthin.
Biological & pharmaceutical bulletin, 2017Co-Authors: Gyujin Park, Minoru Sugiura, Tetsuhiro Horie, Kazuya Fukasawa, Kakeru Ozaki, Yuki Onishi, Takashi Kanayama, Takashi Iezaki, Katsuyuki Kaneda, Eiichi HinoiAbstract:β-Cryptoxanthin, which is primarily obtained from citrus fruits such as Satsuma mandarins, is a major carotenoid routinely found in human serum. Recently, we demonstrated that daily oral intake of β-Cryptoxanthin prevented ovariectomy-induced bone loss and ameliorated neuropathic pain in mice. Although β-Cryptoxanthin exerts preventive effects on various lifestyle-related diseases, there have been no studies on the effect of β-Cryptoxanthin on the development of osteoarthritis, the most common degenerative joint disease, which frequently leads to loss of ability and stiffness in the elderly. Here we showed that daily oral administration of β-Cryptoxanthin significantly prevented the development of osteoarthritis developed by surgically inducing knee joint instability in mice in vivo. Furthermore, in vitro experiments revealed that β-Cryptoxanthin markedly inhibited the expression of inflammatory cytokines and enzymes critical for the degradation of the extracellular matrix in primary chondrocytes. Our results suggest that oral supplementation of β-Cryptoxanthin would be beneficial for the maintenance of joint health and as prophylaxis against osteoarthritis.
Satoshi Uchiyama - One of the best experts on this subject based on the ideXlab platform.
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Osteoporosis Prevention by β-Cryptoxanthin
2008Co-Authors: Masayoshi Yamaguchi, Satoshi UchiyamaAbstract:Pharmacologic and nutritional factors may play a role in the prevention of osteoporosis with aging. P-Cryptoxanthin, a carotenoid, is abundant in Satsuma mandarin orange. Among various carotenoid including p-Cryptoxanthin, lutein, lycopene, β-carotene, astaxanthin, and rutin, P-Cryptoxanthin has been found to have a unique anabolic effect on bone calcification. P-Cryptoxanthin has stimulatory effects on osteoblastic bone formation and inhibitory effects on osteoclastic bone resorption in vitro. β-Cryptoxanthin has an effect on the gene expression of various proteins which are related to osteoblastic bone formation and osteoclastic bone resorption. Oral administration of P-Cryptoxanthin has been shown to have the anabolic effect on bone components in young and aged rats, the preventive effect on bone loss in streptozotocin-diabetic rats and ovariectomy-induced bone loss. Moreover, the intake of β-Cryptoxanthin-reinforced juice for longer periods has been shown to have stimulatory effects on bone formation and inhibitory effects on bone resorption in healthy human and postmenopausal women as estimated based on serum biochemical markers of bone metabolism in vivo. The intake of dietary β-Cryptoxanthin may have a preventive effect on osteoporosis.
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effect of β crytoxanthin on circulating bone metabolic markers intake of juice citrus unshiu supplemented with β Cryptoxanthin has an effect in menopausal women
Journal of Health Science, 2006Co-Authors: Masayoshi Yamaguchi, Aki Igarashi, Satoshi Uchiyama, Seiichi Morita, Kuniaki Sugawara, Takashi Sumida, Hiroshi Ogawa, Masahito Nishitani, Yoshitaka KajimotoAbstract:The effects of prolonged intake of juice prepared from Satsuma mandarin (Citrus unshiu MARC.) containing β-Cryptoxanthin on circulating biochemical markers of bone metabolism in subjects, including menopausal woman, were investigated. Ninety volunteers, aged 27-65 years (19 men and 71 women), were enrolled in this study. The 71 females included 35 premenopausal women (ages, 27-50 years) and 36 postmenopausal women (ages, 46-65 years). Volunteers were divided into four groups; placebo juice without β-cyptoxanthin (5 men and 19 women), juice containing β-cyptoxanthin at 1.5 mg/200 ml of juice/day (4 men and 17 women), 3.0 mg/day (5 men and 17 women), and 6.0 mg/day (5 men and 18 women). Placebo or juice (200 ml) was ingested once a day for 28 or 56 days. Serum β-Cryptoxanthin concentrations were significantly increased after the intake of juice containing β-Cryptoxanthin (1.5, 3.0, or 6.0 mg/day) for 28 or 56 days, and the increases were dose-dependent. Bone-specific alkaline phosphatase and γ-carboxylated osteocalcin are serum bone markers of bone formation, and bone tartrate-resistant acid phosphatase (TRACP) and N-telopeptides of type I collagen are markers of bone resorption. Bone-specific alkaline phosphatase activity was significantly increased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 56 days as compared with the value obtained before intake. γ-Carboxylated osteocalcin concentration was significantly increased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 28 or 56 days as compared with the value obtained before intake or after the intake of placebo juice. Serum TRACP activity and type I collagen N-telopeptide concentration were significantly decreased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 28 or 56 days as compared with the value obtained before intake or after intake of placebo juice, and significant decreases were also seen after the intake of 1.5 mg/day β-Cryptoxanthin as compared with the value obtained before intake. In menopausal women, bone-specific alkaline phosphatase activity and γ-carboxylated osteocalcin concentration were significantly increased after the intake of juice containing β-Cryptoxanthin (3.0 or 6.0 mg/day) for 56 days as compared with the value obtained after placebo intake. Also, this intake caused a significant decrease in bone TRACP activity. Meanwhile, serum calcium, inorganic phosphorous, and parathyroid hormone (intact) were not changed after the intake of β-Cryptoxanthin-containing juice for 28 or 56 days. This study demonstrates that the prolonged intake of juice fortified with β-Cryptoxanthin has stimulatory effects on bone formation and inhibitory effects on bone resorption in humans, and that the intake has an effect in menopausal women.
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β Cryptoxanthin stimulates apoptotic cell death and suppresses cell function in osteoclastic cells change in their related gene expression
Journal of Cellular Biochemistry, 2006Co-Authors: Satoshi Uchiyama, Masayoshi YamaguchiAbstract:The effect of β-Cryptoxanthin, a kind of carotenoid, on osteoclastic cells in mouse marrow culture system in vitro was investigated. The macrophage colony-stimulating factor (M-CSF)-dependent bone marrow macrophages were cultured in the presence of M-CSF (10 ng/ml) and receptor activator of NF-κB ligand (RANKL; 25 ng/ml) for 4 days. The osteoclastic cells formed were further cultured in medium containing either vehicle or β-Cryptoxanthin (10−8–10−6 M) with or without M-CSF (10 ng/ml) and RANKL (50 ng/ml) for 24–72 h. Osteoclastic cells were significantly decreased with culture of β-Cryptoxanthin (10−7 or 10−6 M) with or without M-CSF and RANKL for 24, 48, or 72 h. β-Cryptoxanthin (10−8 M)-induced decrease in osteoclastic cells were significantly inhibited in the presence of caspase-3 inhibitor (10−8 or 10−7 M). Agarose gel electrophoresis showed the presence of low-molecular-weight deoxyribonucleic acid (DNA) fragments of adherent cells cultured with β-Cryptoxanthin (10−7 or 10−6 M) for 24 or 48 h, indicating that the carotenoid induces apoptotic cell death. Apoptosis-related gene expression was determined using reverse transcription-polymerase chain reaction (RT-PCR). Culture with β-Cryptoxanthin (10−7 or 10−6 M) for 24 or 48 h caused a significant increase in caspase-3 mRNA expression in the presence or absence of M-CSF and RANKL, while Bcl-2 and Apaf-2 mRNA expressions were significantly increased with culture of β-Cryptoxanthin (10−7 or 10−6 M) without M-CSF and RANKL for 24 or 48 h. Akt-1 mRNA expression was not significantly changed with culture of the carotenoid (10−7 or 10−6 M) for 24 or 48 h. Moreover, tartrate-resistant acid phosphatase (TRACP) activity, or TRACP and cathepsin K mRNA expressions were significantly decreased with culture of β-Cryptoxanthin (10−6 M) in the presence or absence of M-CSF and RANKL for 48 h. This study demonstrates that β-Cryptoxanthin has stimulatory effects on apoptotic cell death and suppressive effects on osteoclastic cell function. J. Cell. Biochem. 98: 1185–1195, 2006. © 2006 Wiley-Liss, Inc.
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β‐Cryptoxanthin stimulates apoptotic cell death and suppresses cell function in osteoclastic cells: Change in their related gene expression
Journal of cellular biochemistry, 2006Co-Authors: Satoshi Uchiyama, Masayoshi YamaguchiAbstract:The effect of β-Cryptoxanthin, a kind of carotenoid, on osteoclastic cells in mouse marrow culture system in vitro was investigated. The macrophage colony-stimulating factor (M-CSF)-dependent bone marrow macrophages were cultured in the presence of M-CSF (10 ng/ml) and receptor activator of NF-κB ligand (RANKL; 25 ng/ml) for 4 days. The osteoclastic cells formed were further cultured in medium containing either vehicle or β-Cryptoxanthin (10−8–10−6 M) with or without M-CSF (10 ng/ml) and RANKL (50 ng/ml) for 24–72 h. Osteoclastic cells were significantly decreased with culture of β-Cryptoxanthin (10−7 or 10−6 M) with or without M-CSF and RANKL for 24, 48, or 72 h. β-Cryptoxanthin (10−8 M)-induced decrease in osteoclastic cells were significantly inhibited in the presence of caspase-3 inhibitor (10−8 or 10−7 M). Agarose gel electrophoresis showed the presence of low-molecular-weight deoxyribonucleic acid (DNA) fragments of adherent cells cultured with β-Cryptoxanthin (10−7 or 10−6 M) for 24 or 48 h, indicating that the carotenoid induces apoptotic cell death. Apoptosis-related gene expression was determined using reverse transcription-polymerase chain reaction (RT-PCR). Culture with β-Cryptoxanthin (10−7 or 10−6 M) for 24 or 48 h caused a significant increase in caspase-3 mRNA expression in the presence or absence of M-CSF and RANKL, while Bcl-2 and Apaf-2 mRNA expressions were significantly increased with culture of β-Cryptoxanthin (10−7 or 10−6 M) without M-CSF and RANKL for 24 or 48 h. Akt-1 mRNA expression was not significantly changed with culture of the carotenoid (10−7 or 10−6 M) for 24 or 48 h. Moreover, tartrate-resistant acid phosphatase (TRACP) activity, or TRACP and cathepsin K mRNA expressions were significantly decreased with culture of β-Cryptoxanthin (10−6 M) in the presence or absence of M-CSF and RANKL for 48 h. This study demonstrates that β-Cryptoxanthin has stimulatory effects on apoptotic cell death and suppressive effects on osteoclastic cell function. J. Cell. Biochem. 98: 1185–1195, 2006. © 2006 Wiley-Liss, Inc.
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Oral Administration in Combination with Zinc Enhances β-Cryptoxanthin-Induced Anabolic Effects on Bone Components in the Femoral Tissues of Rats in Vivo
Biological & pharmaceutical bulletin, 2006Co-Authors: Masayoshi Yamaguchi, Satoshi Uchiyama, Kaori Ishiyama, Ken HashimotoAbstract:The effects of combined beta-Cryptoxanthin and zinc on bone components in the femoral-diaphyseal (cortical bone) and -metaphyseal (trabecular bone) tissues of rats in vivo were investigated. Rats were orally administered either vehicle, beta-Cryptoxanthin (5 or 10 microg/100 g body weight), zinc sulfate (0.1 or 0.5 mg Zn/100 g), or their combination once a day for 7 d. Calcium content, alkaline phosphatase activity, and DNA content in the femoral-diaphyseal tissues was not significantly altered by the administration of beta-Cryptoxanthin (5 microg/100 g) or zinc (0.1 or 0.5 mg/100 g). Combined administration of beta-Cryptoxanthin (5 microg/100 g) and zinc (0.1 or 0.5 mg/100 g) caused a synergistic increase in calcium content, alkaline phosphatase activity, and DNA content in the diaphyseal tissues. The effect of beta-Cryptoxanthin (5 or 10 microg/100 g) in increasing calcium and DNA contents in the metaphyseal tissues was significantly enhanced by the combined administration of zinc (0.1 or 0.5 mg/100 g), but did not have a significant effect on the metaphyseal components. The metaphyseal alkaline phosphatase activity was markedly increased by the combination of beta-Cryptoxanthin (5 microg/100 g) and zinc (0.1 or 0.5 mg/100 g). This study demonstrates that the oral administration of the combination of zinc at lower doses synergistically enhances beta-Cryptoxanthin-induced anabolic effects on bone components in the femoral tissues of rats in vivo.
Chun Liu - One of the best experts on this subject based on the ideXlab platform.
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Abstract 832: Effects of beta-Cryptoxanthin on cigarette smoke-induced lung oxidative damage, inflammation and activation of NF-kappa B and AP-1
Prevention Research, 2011Co-Authors: Chun Liu, Roderick T. Bronson, Robert M. Russell, Xiang-dong WangAbstract:Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL In epidemiologic studies, high intake of beta-Cryptoxanthin has been associated with a decreased risk of lung cancer, particularly among current smokers. However, data are not available from well-controlled animal studies to examine the effects of beta-Cryptoxanthin on cigarette smoke-induced lung lesions, and the biological mechanisms by which beta-Cryptoxanthin might affect lung carcinogenesis. We evaluated the effects of beta-Cryptoxanthin supplementation on cigarette smoke-induced squamous metaplasia, inflammation, and changes in protein levels of pro-inflammatory cytokine [tumor necrosis factor alpha (TNF-alpha)] and transcription factors [nuclear factor kappa B (NF-kappa B) and activator protein-1 (AP-1)], as well as on smoke-induced oxidative DNA damage [8-hydroxy-2′-deoxyguanosine (8-OHdG)] in the lung tissue of ferrets. Thirty six male ferrets were assigned to cigarette smoke exposure or to no exposure and to no dose, low-dose, or high-dose beta-Cryptoxanthin supplementation (2 × 3 factorial design) for 3 months. Beta-Cryptoxanthin supplementation dose-dependently increased plasma and lung beta-Cryptoxanthin levels in ferrets, whereas cigarette smoke exposure lowered plasma and lung beta-Cryptoxanthin levels. Beta-Cryptoxanthin at both doses significantly decreased smoke-induced lung squamous metaplasia and inflammation. Beta-Cryptoxanthin also substantially reduced smoke-elevated TNF-alpha levels in alveolar, bronchial, bronchiolar and airway serous/mucous gland epithelial cells and in lung macrophages. Moreover, beta-Cryptoxanthin decreased smoke-induced activation of NF-kappa B and expression of AP-1 and 8-OHdG. The beneficial effects of beta-Cryptoxanthin were stronger for high-dose beta-Cryptoxanthin than for low-dose beta-Cryptoxanthin. Data from this study indicate that beta-Cryptoxanthin provides a beneficial effect against cigarette smoke-induced inflammation, oxidative DNA damage and squamous metaplasia in the lungs. Beta-Cryptoxanthin may prevent lung carcinogenesis through its inhibitive effects on lung inflammation and oxidative DNA damage. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 832. doi:10.1158/1538-7445.AM2011-832
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β-Cryptoxanthin supplementation prevents cigarette smoke-induced lung inflammation, oxidative damage and squamous metaplasia in ferrets
Cancer prevention research (Philadelphia Pa.), 2011Co-Authors: Chun Liu, Roderick T. Bronson, Robert M. Russell, Xiang-dong WangAbstract:In epidemiologic studies, high intake of β-Cryptoxanthin has been associated with a decreased risk of lung cancer, particularly among current smokers. However, data are not available from well-controlled animal studies to examine the effects of β-Cryptoxanthin on cigarette smoke-induced lung lesions, and the biological mechanisms by which β-Cryptoxanthin might affect lung carcinogenesis. We evaluated the effects of β-Cryptoxanthin supplementation on cigarette smoke-induced squamous metaplasia, inflammation, and changes in protein levels of proinflammatory cytokine [tumor necrosis factor alpha (TNFα)] and transcription factors [nuclear factor kappa B (NF-κB) and activator protein-1 (AP-1)], as well as on smoke-induced oxidative DNA damage [8-hydroxy-2'-deoxyguanosine (8-OHdG)] in the lung tissue of ferrets. Thirty-six male ferrets were assigned to cigarette smoke exposure or no exposure and to low-dose, or high-dose β-Cryptoxanthin, or no dose (2 × 3 factorial design) for 3 months. β-Cryptoxanthin supplementation dose-dependently increased plasma and lung β-Cryptoxanthin levels in ferrets, whereas cigarette smoke exposure lowered plasma and lung β-Cryptoxanthin levels. β-Cryptoxanthin at both doses significantly decreased smoke-induced lung squamous metaplasia and inflammation. β-Cryptoxanthin also substantially reduced smoke-elevated TNFα levels in alveolar, bronchial, bronchiolar, and bronchial serous/mucous gland epithelial cells and in lung macrophages. Moreover, β-Cryptoxanthin decreased smoke-induced activation of NF-κB, expression of AP-1 and levels of 8-OHdG. The beneficial effects of β-Cryptoxanthin were stronger for high-dose β-Cryptoxanthin than for low-dose β-Cryptoxanthin. Data from this study indicate that β-Cryptoxanthin provides a beneficial effect against cigarette smoke-induced inflammation, oxidative DNA damage and squamous metaplasia in the lungs.
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Abstract LB-430: Chemopreventive effect of beta-Cryptoxanthin on smoke-induced lung inflammation and lesions in ferrets
Prevention Research, 2010Co-Authors: Chun Liu, Roderick T. Bronson, Xiang-dong WangAbstract:Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC Background: High intake of beta-Cryptoxanthin has been associated with a decreased risk of lung cancer, particularly among current smokers in epidemiological studies. However, there are no available data from well-controlled animal studies to examine the effect of beta-Cryptoxanthin on smoke-induced lung lesions and potential biological mechanisms by which beta-Cryptoxanthin affects lung carcinogenesis. Using a ferret animal model, we evaluated the effect of beta-Cryptoxanthin supplementation in both low-and high-dose (7.5 mcg/kg body weight per day vs. 37.5 mcg/kg body weight per day in ferrets which are equivalent to 104 mcg/day vs. 520 mcg/day, respectively, in the humans) on the cigarette smoke-induced pro-inflammatory cytokine TNF alpha, inflammation and squamous metaplasia in the lung. Methods: Thirty six male ferrets were assigned to one of six groups (n = 6 in each group) for 9 weeks as follows: 1) control; 2) cigarette smoke exposed; 3) low-dose beta-Cryptoxanthin (7.5 mcg/kg body weight per day); 4) high-dose beta-Cryptoxanthin (37.5 mcg/kg body weight per day); 5) cigarette smoke-exposed plus low-dose beta-Cryptoxanthin; and 6) cigarette smoke-exposed plus high-dose beta-Cryptoxanthin. Lung TNF alpha was measured by immunohistochemical staining. Lung inflammation and squamous metaplasia were quantified by histopathological evaluations. Results: Beta-Cryptoxanthin supplementation dose-dependently increased plasma and lung beta-Cryptoxanthin levels in ferrets. Cigarette smoke exposure lowered plasma and lung beta-Cryptoxanthin levels. Treatment with beta-Cryptoxanthin substantially reduced the cigarette smoke-elevated TNF alpha levels in bronchial/bronchiolar epithelial cells, alveolar epithelial cells, airway serous/mucous glands and lung macrophages. Beta-Cryptoxanthin at both doses also significantly decreased the cigarette smoke-induced lung inflammation. In addition, both doses of beta-Cryptoxanthin significantly lowered the incidence of lung squamous metaplasia induced by cigarette smoke exposure. There were no detectable squamous metaplasia in the groups treated with beta-Cryptoxanthin alone and in the control group. Conclusions: Beta-Cryptoxanthin may provide a beneficial effect against the smoke-induced lung inflammation and lung lesions. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-430.