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Sami Fattouch - One of the best experts on this subject based on the ideXlab platform.
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concentration dependent effects of commonly used pesticides on activation versus inhibition of the quince Cydonia oblonga polyphenol oxidase
Food and Chemical Toxicology, 2010Co-Authors: Sami Fattouch, Nejib Marzouki, Faten Raboudifattouch, Jose Vicente Gil Ponce, Josep V Forment, Dunja Lukovic, Daniel Ramon VidalAbstract:Abstract Polyphenol oxidase (PPO) catalyzes the oxidation of o -diphenols to their respective quinones which undergo autopolymerization and form dark pigments. The interaction of PPO with various substrates and effectors remains the focus of intensive investigations due to the enzyme’s key role in pigments biosynthesis including animal melanogenesis and fruit/fungi enzymatic browning. In this study, the effect of a range of commonly used pesticides on the enzyme activity has been evaluated using the purified quince ( Cydonia oblonga Miller) PPO. The biochemical analysis showed that, in the presence of high pesticide concentrations, the enzyme was competitively inhibited, particularly with benomyl, carbaryl, deltamethrine and parathion methyl for which inhibition constants ( K i ) were 8.3, 5.7, 12 and 4 μM, respectively. At lower pesticide concentrations (2–10 μM), however, the catecholase activity was significantly activated ( p in silico structure-based analyses, known as computational docking, highlighted the nature of the PPO-pesticides interactions and confirmed the in vitro observations. Catechol substrate and parathion methyl inhibitor showed lower total energy scores of −120.06 and −117.43 kcal mol −1 , indicating that these ligands had higher PPO-binding affinities. The obtained data bring to light new pesticide functional features of great interest in the medicinal, agro-chemical and environmental circles.
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damage inhibition during refrigerated storage of mackerel scomber scombrus fillets by a presoaking in quince Cydonia oblonga polyphenolic extract
International Journal of Food Science and Technology, 2008Co-Authors: Sami Fattouch, Faten Raboudifattouch, Saloua Sadok, Mourad Ben SlamaAbstract:Summary The effect of quince (Cydonia oblonga Miller) polyphenolic extract on preserving mackerel (Scomber scombrus) fillets during refrigerated (4 °C) storage for 11 days was investigated. The quince extract, mainly consisted of procyanidin B dimer (50.8%) and hydroxycinnamic acids (36.62%), was found to quench 2,2-diphenyl-1-picrylhydrazyl radicals by nearly 59.3%. As deduced from the lower peroxide value of the fillet fat fraction and the inhibition of thiobarbituric acid reactive substances formation, the quince extract (8.9 ± 0.4 mg phenolics mL−1) was found to prevent fish oil from oxidative deterioration compared to control samples. Moreover, the extract was found to be active for in vitro inhibiting the growth of a range of food-borne bacteria, including Vibrio fluvialis, a halophilic bacterium responsible for a relevant number of sporadic gastroenteritis attributed to contaminated seafood. The minimal inhibition and bactericide concentration (MIC, MBC) values were about 0.37–1.2 mg of polyphenols mL−1. In a second experiment, following cold smoking of fillets, sensory attributes firmness, astringency, acidity, colour and odour were analysed. The use of multivariate statistical methods (cluster analysis and principal component analysis) revealed no significant differences between samples pre-treated and non-pre-treated (control) with the quince-phenolic extract.
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Antimicrobial activity of Tunisian quince (Cydonia oblonga Miller) pulp and peel polyphenolic extracts.
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Sami Fattouch, Pierluigi Caboni, Valentina Coroneo, Carlo Ignazio Giovanni Tuberoso, Alberto Angioni, Sandro Dessi, Nejib Marzouki, Paolo CabrasAbstract:Quince (Cydonia oblonga Miller) fruit aqueous acetone extracts were evaluated. High-performance liquid chromatography−diode array detection and electrospray ionization−mass spectrometry were used for the identification and quantification of the phenolic compounds. The total phenolic content of the pulp and peel parts ranged from 37 to 47 and 105 to 157 mg/100 g of fresh weight, respectively. Chlorogenic acid (5-O-caffeoylquinic acid) was the most abundant phenolic compound in the pulp (37%), whereas rutin (quercetin 3-O-rutinoside) was the main one in the peel (36%). The radical scavenging potential of the extracts was determined and compared with that of synthetic antioxidants. The stronger properties corresponded to those obtained from peel material with a 70−80% inhibitory effect on DPPH radicals. The antimicrobial activity of the extracts against different microorganism strains was also investigated. Quince peel extract was the most active for inhibiting bacteria growth with minimum inhibitory and bac...
Branca M. Silva - One of the best experts on this subject based on the ideXlab platform.
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Targeted metabolites and biological activities of Cydonia oblonga Miller leaves
Food Research International, 2012Co-Authors: Andreia Oliveira, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Rossana M. Costa, Ana S. Magalhães, Márcia Carvalho, Branca M. SilvaAbstract:Abstract Natural products continue to play a significant role in drug discovery and development processes, and many plants have already provided valuable clues for potentially bioactive compounds. Additionally, in recent years it has become evident that significant health risks and benefits are associated with dietary food choice. Nutritional studies recommend the regular consumption of fruits and vegetables, which constitute an essential part of the Mediterranean diet, to favor a healthy life. Several works have showed that quince tree (Cydonia oblonga Miller) is a good and low cost natural source of metabolites with interesting biological properties. Extensive research has been performed on the metabolic profile and biological potential of this species. In particular, quince leaves revealed to constitute a promising natural source of bioactive phytochemicals, suitable for application in nutritional/pharmaceutical fields. In this review phenolics and organic acids composition of quince leaf is overviewed, as well as its antioxidant and antiproliferative properties, in chemical and cellular in vitro systems. Comparisons with green tea, considered by the scientific community as an effective natural antioxidant, were established.
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first report on Cydonia oblonga miller anticancer potential differential antiproliferative effect against human kidney and colon cancer cells
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Márcia Carvalho, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, Renata Silva, Maria De Lourdes BastosAbstract:The present study reports the phenolic profile and antiproliferative properties of quince (Cydonia oblonga Miller) leaf and fruit (pulp, peel, and seed) against human kidney and colon cancer cells. The phenolic profiles of quince methanolic extracts were determined by high-performance liquid chromatography (HPLC)/diode array detector (DAD). 5-O-Caffeoylquinic acid was always one of the two major phenolic compounds present in all extracts, except for seed. Our results revealed that quince leaf and fruit extracts exhibited distinctive antiproliferative activities. The extracts from quince leaf showed concentration-dependent growth inhibitory activity toward human colon cancer cells (IC(50) = 239.7 +/- 43.2 microg/mL), while no effect was observed in renal adenocarcinoma cells. Concerning the fruit, seed extracts exhibited no effect on colon cancer cell growth, whereas strong antiproliferative efficiency against renal cancer cells was observed for the highest concentration assayed (500 microg/mL). The antiproliferative activity of pulp and peel extracts was low or absent in the selected range of extract concentrations. This is the first report showing that C. oblonga may be useful as a cancer chemopreventive and/or chemotherapeutic agent.
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protective effect of quince Cydonia oblonga miller fruit against oxidative hemolysis of human erythrocytes
Food and Chemical Toxicology, 2009Co-Authors: Ana S. Magalhães, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Márcia CarvalhoAbstract:Abstract The aim of this study was to determine the phenolic content and evaluate the antioxidant activity of quince ( Cydonia oblonga ) fruit. For this purpose, fruits were separated into pulps, peels and seeds and methanolic extracts were prepared. The phenolic profiles were determined by HPLC/UV and antioxidant properties were studied for their ability to quench the stable free radical 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. The main phenolic compounds were 5- O -caffeoylquinic acid for pulp and peel (57% and 29%, respectively) and stellarin-2 for seed (18%). Total phenolics content was 2.5, 6.3 and 0.4 g/kg of methanolic extract for pulp, peel and seed, respectively. Pulp and peel extracts showed similar DPPH free radical scavenging activities (EC 50 of 0.6 and 0.8 mg/ml, respectively), while seed extract presented much lower antioxidant potential (EC 50 of 12.2 mg/ml). Under the oxidative action of AAPH, pulp and peel extracts showed significant protection of the erythrocyte membrane from hemolysis, in a time- and concentration-dependent manner. Seed extracts by themselves induced extensive hemolysis. These results indicate higher antioxidant activity for certain parts of quince fruit, namely pulp and peel, that may therefore represent accessible sources of natural antioxidants with potential application in nutritional/pharmaceutical fields, as preventive or therapeutic agents in diseases in which free radicals are implicated.
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Organic acids composition of Cydonia oblonga Miller leaf.
Food Chemistry, 2008Co-Authors: Andreia P. Oliveira, Paula B. Andrade, Patrícia Valentão, Rosa M. Seabra, José Alberto Pereira, Branca M. SilvaAbstract:Abstract Organic acid profiles of 36 Cydonia oblonga Miller leaf samples, from three different geographical origins of northern (Braganca and Carrazeda de Ansiaes) and central Portugal (Covilha), harvested in three collection months (June, August and October of 2006), were determined by HPLC/UV (214 nm). Quince leaves presented a common organic acid profile, composed of six constituents: oxalic, citric, malic, quinic, shikimic and fumaric acids. C. oblonga leaves total organic acid content varied from 1.6 to 25.8 g/kg dry matter (mean value of 10.5 g/kg dry matter). Quinic acid was the major compound (72.2%), followed by citric acid (13.6%). Significant differences were found in malic and quinic acids relative abundances and total organic acid contents according to collection time, which indicates a possible use of these compounds as maturity markers. Between June and August seems to be the best period to harvest quince leaves for preparation of decoctions or infusions, since organic acids total content is higher in this season.
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phenolic profile of Cydonia oblonga miller leaves
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Andreia P. Oliveira, Paula B. Andrade, Patrícia Valentão, Rosa M. Seabra, José Alberto Pereira, Branca M. SilvaAbstract:Cydonia oblonga Miller leaves phenolic compounds were analyzed by reversed-phase HPLC/DAD and HPLC/UV. Qualitative and quantitative analysis of phenolics were carried out in a total of 36 samples of quince leaves from three different geographical origins of Northern (Braganca and Carrazeda de Ansiaes) and Central Portugal (Covilha) and three collection months (June, August, and October of 2006). These leaves presented a common phenolic profile composed by nine compounds: 3- O-, 4- O- and 5- O-caffeoylquinic acids, 3,5- O-dicaffeoylquinic acid, quercetin-3- O-galactoside, quercetin-3- O-rutinoside, kaempferol-3- O-glycoside, kaempferol-3- O-glucoside, and kaempferol-3- O-rutinoside. 5- O-caffeoylquinic acid was the major phenolic compound (36.2%), followed by quercetin 3- O-rutinoside (21.1%). Quince leaves are characterized by higher relative contents of kaempferol derivatives than fruits (pulps, peels, and seeds), especially in what concerns kaempferol-3- O-rutinoside (12.5%). C. oblonga leaves total phenolic content was very high, varying from 4.9 to 16.5 g/kg dry matter (mean value of 10.3 g/kg dry matter), indicating that these leaves can be used as a good and cheap source of bioactive constituents. Significantly differences were observed in 3- O-caffeoylquinic and 3,5- O-dicaffeoylquinic acids contents, according to geographical provenance and harvesting month, suggesting a possible use of these compounds as geographical origin and/or maturity markers.
Márcia Carvalho - One of the best experts on this subject based on the ideXlab platform.
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Targeted metabolites and biological activities of Cydonia oblonga Miller leaves
Food Research International, 2012Co-Authors: Andreia Oliveira, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Rossana M. Costa, Ana S. Magalhães, Márcia Carvalho, Branca M. SilvaAbstract:Abstract Natural products continue to play a significant role in drug discovery and development processes, and many plants have already provided valuable clues for potentially bioactive compounds. Additionally, in recent years it has become evident that significant health risks and benefits are associated with dietary food choice. Nutritional studies recommend the regular consumption of fruits and vegetables, which constitute an essential part of the Mediterranean diet, to favor a healthy life. Several works have showed that quince tree (Cydonia oblonga Miller) is a good and low cost natural source of metabolites with interesting biological properties. Extensive research has been performed on the metabolic profile and biological potential of this species. In particular, quince leaves revealed to constitute a promising natural source of bioactive phytochemicals, suitable for application in nutritional/pharmaceutical fields. In this review phenolics and organic acids composition of quince leaf is overviewed, as well as its antioxidant and antiproliferative properties, in chemical and cellular in vitro systems. Comparisons with green tea, considered by the scientific community as an effective natural antioxidant, were established.
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first report on Cydonia oblonga miller anticancer potential differential antiproliferative effect against human kidney and colon cancer cells
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Márcia Carvalho, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, Renata Silva, Maria De Lourdes BastosAbstract:The present study reports the phenolic profile and antiproliferative properties of quince (Cydonia oblonga Miller) leaf and fruit (pulp, peel, and seed) against human kidney and colon cancer cells. The phenolic profiles of quince methanolic extracts were determined by high-performance liquid chromatography (HPLC)/diode array detector (DAD). 5-O-Caffeoylquinic acid was always one of the two major phenolic compounds present in all extracts, except for seed. Our results revealed that quince leaf and fruit extracts exhibited distinctive antiproliferative activities. The extracts from quince leaf showed concentration-dependent growth inhibitory activity toward human colon cancer cells (IC(50) = 239.7 +/- 43.2 microg/mL), while no effect was observed in renal adenocarcinoma cells. Concerning the fruit, seed extracts exhibited no effect on colon cancer cell growth, whereas strong antiproliferative efficiency against renal cancer cells was observed for the highest concentration assayed (500 microg/mL). The antiproliferative activity of pulp and peel extracts was low or absent in the selected range of extract concentrations. This is the first report showing that C. oblonga may be useful as a cancer chemopreventive and/or chemotherapeutic agent.
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protective effect of quince Cydonia oblonga miller fruit against oxidative hemolysis of human erythrocytes
Food and Chemical Toxicology, 2009Co-Authors: Ana S. Magalhães, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Márcia CarvalhoAbstract:Abstract The aim of this study was to determine the phenolic content and evaluate the antioxidant activity of quince ( Cydonia oblonga ) fruit. For this purpose, fruits were separated into pulps, peels and seeds and methanolic extracts were prepared. The phenolic profiles were determined by HPLC/UV and antioxidant properties were studied for their ability to quench the stable free radical 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. The main phenolic compounds were 5- O -caffeoylquinic acid for pulp and peel (57% and 29%, respectively) and stellarin-2 for seed (18%). Total phenolics content was 2.5, 6.3 and 0.4 g/kg of methanolic extract for pulp, peel and seed, respectively. Pulp and peel extracts showed similar DPPH free radical scavenging activities (EC 50 of 0.6 and 0.8 mg/ml, respectively), while seed extract presented much lower antioxidant potential (EC 50 of 12.2 mg/ml). Under the oxidative action of AAPH, pulp and peel extracts showed significant protection of the erythrocyte membrane from hemolysis, in a time- and concentration-dependent manner. Seed extracts by themselves induced extensive hemolysis. These results indicate higher antioxidant activity for certain parts of quince fruit, namely pulp and peel, that may therefore represent accessible sources of natural antioxidants with potential application in nutritional/pharmaceutical fields, as preventive or therapeutic agents in diseases in which free radicals are implicated.
Patrícia Valentão - One of the best experts on this subject based on the ideXlab platform.
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Targeted metabolites and biological activities of Cydonia oblonga Miller leaves
Food Research International, 2012Co-Authors: Andreia Oliveira, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Rossana M. Costa, Ana S. Magalhães, Márcia Carvalho, Branca M. SilvaAbstract:Abstract Natural products continue to play a significant role in drug discovery and development processes, and many plants have already provided valuable clues for potentially bioactive compounds. Additionally, in recent years it has become evident that significant health risks and benefits are associated with dietary food choice. Nutritional studies recommend the regular consumption of fruits and vegetables, which constitute an essential part of the Mediterranean diet, to favor a healthy life. Several works have showed that quince tree (Cydonia oblonga Miller) is a good and low cost natural source of metabolites with interesting biological properties. Extensive research has been performed on the metabolic profile and biological potential of this species. In particular, quince leaves revealed to constitute a promising natural source of bioactive phytochemicals, suitable for application in nutritional/pharmaceutical fields. In this review phenolics and organic acids composition of quince leaf is overviewed, as well as its antioxidant and antiproliferative properties, in chemical and cellular in vitro systems. Comparisons with green tea, considered by the scientific community as an effective natural antioxidant, were established.
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first report on Cydonia oblonga miller anticancer potential differential antiproliferative effect against human kidney and colon cancer cells
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Márcia Carvalho, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, Renata Silva, Maria De Lourdes BastosAbstract:The present study reports the phenolic profile and antiproliferative properties of quince (Cydonia oblonga Miller) leaf and fruit (pulp, peel, and seed) against human kidney and colon cancer cells. The phenolic profiles of quince methanolic extracts were determined by high-performance liquid chromatography (HPLC)/diode array detector (DAD). 5-O-Caffeoylquinic acid was always one of the two major phenolic compounds present in all extracts, except for seed. Our results revealed that quince leaf and fruit extracts exhibited distinctive antiproliferative activities. The extracts from quince leaf showed concentration-dependent growth inhibitory activity toward human colon cancer cells (IC(50) = 239.7 +/- 43.2 microg/mL), while no effect was observed in renal adenocarcinoma cells. Concerning the fruit, seed extracts exhibited no effect on colon cancer cell growth, whereas strong antiproliferative efficiency against renal cancer cells was observed for the highest concentration assayed (500 microg/mL). The antiproliferative activity of pulp and peel extracts was low or absent in the selected range of extract concentrations. This is the first report showing that C. oblonga may be useful as a cancer chemopreventive and/or chemotherapeutic agent.
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protective effect of quince Cydonia oblonga miller fruit against oxidative hemolysis of human erythrocytes
Food and Chemical Toxicology, 2009Co-Authors: Ana S. Magalhães, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Márcia CarvalhoAbstract:Abstract The aim of this study was to determine the phenolic content and evaluate the antioxidant activity of quince ( Cydonia oblonga ) fruit. For this purpose, fruits were separated into pulps, peels and seeds and methanolic extracts were prepared. The phenolic profiles were determined by HPLC/UV and antioxidant properties were studied for their ability to quench the stable free radical 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. The main phenolic compounds were 5- O -caffeoylquinic acid for pulp and peel (57% and 29%, respectively) and stellarin-2 for seed (18%). Total phenolics content was 2.5, 6.3 and 0.4 g/kg of methanolic extract for pulp, peel and seed, respectively. Pulp and peel extracts showed similar DPPH free radical scavenging activities (EC 50 of 0.6 and 0.8 mg/ml, respectively), while seed extract presented much lower antioxidant potential (EC 50 of 12.2 mg/ml). Under the oxidative action of AAPH, pulp and peel extracts showed significant protection of the erythrocyte membrane from hemolysis, in a time- and concentration-dependent manner. Seed extracts by themselves induced extensive hemolysis. These results indicate higher antioxidant activity for certain parts of quince fruit, namely pulp and peel, that may therefore represent accessible sources of natural antioxidants with potential application in nutritional/pharmaceutical fields, as preventive or therapeutic agents in diseases in which free radicals are implicated.
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Organic acids composition of Cydonia oblonga Miller leaf.
Food Chemistry, 2008Co-Authors: Andreia P. Oliveira, Paula B. Andrade, Patrícia Valentão, Rosa M. Seabra, José Alberto Pereira, Branca M. SilvaAbstract:Abstract Organic acid profiles of 36 Cydonia oblonga Miller leaf samples, from three different geographical origins of northern (Braganca and Carrazeda de Ansiaes) and central Portugal (Covilha), harvested in three collection months (June, August and October of 2006), were determined by HPLC/UV (214 nm). Quince leaves presented a common organic acid profile, composed of six constituents: oxalic, citric, malic, quinic, shikimic and fumaric acids. C. oblonga leaves total organic acid content varied from 1.6 to 25.8 g/kg dry matter (mean value of 10.5 g/kg dry matter). Quinic acid was the major compound (72.2%), followed by citric acid (13.6%). Significant differences were found in malic and quinic acids relative abundances and total organic acid contents according to collection time, which indicates a possible use of these compounds as maturity markers. Between June and August seems to be the best period to harvest quince leaves for preparation of decoctions or infusions, since organic acids total content is higher in this season.
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phenolic profile of Cydonia oblonga miller leaves
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Andreia P. Oliveira, Paula B. Andrade, Patrícia Valentão, Rosa M. Seabra, José Alberto Pereira, Branca M. SilvaAbstract:Cydonia oblonga Miller leaves phenolic compounds were analyzed by reversed-phase HPLC/DAD and HPLC/UV. Qualitative and quantitative analysis of phenolics were carried out in a total of 36 samples of quince leaves from three different geographical origins of Northern (Braganca and Carrazeda de Ansiaes) and Central Portugal (Covilha) and three collection months (June, August, and October of 2006). These leaves presented a common phenolic profile composed by nine compounds: 3- O-, 4- O- and 5- O-caffeoylquinic acids, 3,5- O-dicaffeoylquinic acid, quercetin-3- O-galactoside, quercetin-3- O-rutinoside, kaempferol-3- O-glycoside, kaempferol-3- O-glucoside, and kaempferol-3- O-rutinoside. 5- O-caffeoylquinic acid was the major phenolic compound (36.2%), followed by quercetin 3- O-rutinoside (21.1%). Quince leaves are characterized by higher relative contents of kaempferol derivatives than fruits (pulps, peels, and seeds), especially in what concerns kaempferol-3- O-rutinoside (12.5%). C. oblonga leaves total phenolic content was very high, varying from 4.9 to 16.5 g/kg dry matter (mean value of 10.3 g/kg dry matter), indicating that these leaves can be used as a good and cheap source of bioactive constituents. Significantly differences were observed in 3- O-caffeoylquinic and 3,5- O-dicaffeoylquinic acids contents, according to geographical provenance and harvesting month, suggesting a possible use of these compounds as geographical origin and/or maturity markers.
Paula B. Andrade - One of the best experts on this subject based on the ideXlab platform.
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Targeted metabolites and biological activities of Cydonia oblonga Miller leaves
Food Research International, 2012Co-Authors: Andreia Oliveira, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Rossana M. Costa, Ana S. Magalhães, Márcia Carvalho, Branca M. SilvaAbstract:Abstract Natural products continue to play a significant role in drug discovery and development processes, and many plants have already provided valuable clues for potentially bioactive compounds. Additionally, in recent years it has become evident that significant health risks and benefits are associated with dietary food choice. Nutritional studies recommend the regular consumption of fruits and vegetables, which constitute an essential part of the Mediterranean diet, to favor a healthy life. Several works have showed that quince tree (Cydonia oblonga Miller) is a good and low cost natural source of metabolites with interesting biological properties. Extensive research has been performed on the metabolic profile and biological potential of this species. In particular, quince leaves revealed to constitute a promising natural source of bioactive phytochemicals, suitable for application in nutritional/pharmaceutical fields. In this review phenolics and organic acids composition of quince leaf is overviewed, as well as its antioxidant and antiproliferative properties, in chemical and cellular in vitro systems. Comparisons with green tea, considered by the scientific community as an effective natural antioxidant, were established.
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first report on Cydonia oblonga miller anticancer potential differential antiproliferative effect against human kidney and colon cancer cells
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Márcia Carvalho, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, Renata Silva, Maria De Lourdes BastosAbstract:The present study reports the phenolic profile and antiproliferative properties of quince (Cydonia oblonga Miller) leaf and fruit (pulp, peel, and seed) against human kidney and colon cancer cells. The phenolic profiles of quince methanolic extracts were determined by high-performance liquid chromatography (HPLC)/diode array detector (DAD). 5-O-Caffeoylquinic acid was always one of the two major phenolic compounds present in all extracts, except for seed. Our results revealed that quince leaf and fruit extracts exhibited distinctive antiproliferative activities. The extracts from quince leaf showed concentration-dependent growth inhibitory activity toward human colon cancer cells (IC(50) = 239.7 +/- 43.2 microg/mL), while no effect was observed in renal adenocarcinoma cells. Concerning the fruit, seed extracts exhibited no effect on colon cancer cell growth, whereas strong antiproliferative efficiency against renal cancer cells was observed for the highest concentration assayed (500 microg/mL). The antiproliferative activity of pulp and peel extracts was low or absent in the selected range of extract concentrations. This is the first report showing that C. oblonga may be useful as a cancer chemopreventive and/or chemotherapeutic agent.
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protective effect of quince Cydonia oblonga miller fruit against oxidative hemolysis of human erythrocytes
Food and Chemical Toxicology, 2009Co-Authors: Ana S. Magalhães, Branca M. Silva, Paula B. Andrade, Patrícia Valentão, José Alberto Pereira, Márcia CarvalhoAbstract:Abstract The aim of this study was to determine the phenolic content and evaluate the antioxidant activity of quince ( Cydonia oblonga ) fruit. For this purpose, fruits were separated into pulps, peels and seeds and methanolic extracts were prepared. The phenolic profiles were determined by HPLC/UV and antioxidant properties were studied for their ability to quench the stable free radical 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. The main phenolic compounds were 5- O -caffeoylquinic acid for pulp and peel (57% and 29%, respectively) and stellarin-2 for seed (18%). Total phenolics content was 2.5, 6.3 and 0.4 g/kg of methanolic extract for pulp, peel and seed, respectively. Pulp and peel extracts showed similar DPPH free radical scavenging activities (EC 50 of 0.6 and 0.8 mg/ml, respectively), while seed extract presented much lower antioxidant potential (EC 50 of 12.2 mg/ml). Under the oxidative action of AAPH, pulp and peel extracts showed significant protection of the erythrocyte membrane from hemolysis, in a time- and concentration-dependent manner. Seed extracts by themselves induced extensive hemolysis. These results indicate higher antioxidant activity for certain parts of quince fruit, namely pulp and peel, that may therefore represent accessible sources of natural antioxidants with potential application in nutritional/pharmaceutical fields, as preventive or therapeutic agents in diseases in which free radicals are implicated.
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Organic acids composition of Cydonia oblonga Miller leaf.
Food Chemistry, 2008Co-Authors: Andreia P. Oliveira, Paula B. Andrade, Patrícia Valentão, Rosa M. Seabra, José Alberto Pereira, Branca M. SilvaAbstract:Abstract Organic acid profiles of 36 Cydonia oblonga Miller leaf samples, from three different geographical origins of northern (Braganca and Carrazeda de Ansiaes) and central Portugal (Covilha), harvested in three collection months (June, August and October of 2006), were determined by HPLC/UV (214 nm). Quince leaves presented a common organic acid profile, composed of six constituents: oxalic, citric, malic, quinic, shikimic and fumaric acids. C. oblonga leaves total organic acid content varied from 1.6 to 25.8 g/kg dry matter (mean value of 10.5 g/kg dry matter). Quinic acid was the major compound (72.2%), followed by citric acid (13.6%). Significant differences were found in malic and quinic acids relative abundances and total organic acid contents according to collection time, which indicates a possible use of these compounds as maturity markers. Between June and August seems to be the best period to harvest quince leaves for preparation of decoctions or infusions, since organic acids total content is higher in this season.
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phenolic profile of Cydonia oblonga miller leaves
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Andreia P. Oliveira, Paula B. Andrade, Patrícia Valentão, Rosa M. Seabra, José Alberto Pereira, Branca M. SilvaAbstract:Cydonia oblonga Miller leaves phenolic compounds were analyzed by reversed-phase HPLC/DAD and HPLC/UV. Qualitative and quantitative analysis of phenolics were carried out in a total of 36 samples of quince leaves from three different geographical origins of Northern (Braganca and Carrazeda de Ansiaes) and Central Portugal (Covilha) and three collection months (June, August, and October of 2006). These leaves presented a common phenolic profile composed by nine compounds: 3- O-, 4- O- and 5- O-caffeoylquinic acids, 3,5- O-dicaffeoylquinic acid, quercetin-3- O-galactoside, quercetin-3- O-rutinoside, kaempferol-3- O-glycoside, kaempferol-3- O-glucoside, and kaempferol-3- O-rutinoside. 5- O-caffeoylquinic acid was the major phenolic compound (36.2%), followed by quercetin 3- O-rutinoside (21.1%). Quince leaves are characterized by higher relative contents of kaempferol derivatives than fruits (pulps, peels, and seeds), especially in what concerns kaempferol-3- O-rutinoside (12.5%). C. oblonga leaves total phenolic content was very high, varying from 4.9 to 16.5 g/kg dry matter (mean value of 10.3 g/kg dry matter), indicating that these leaves can be used as a good and cheap source of bioactive constituents. Significantly differences were observed in 3- O-caffeoylquinic and 3,5- O-dicaffeoylquinic acids contents, according to geographical provenance and harvesting month, suggesting a possible use of these compounds as geographical origin and/or maturity markers.
