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Jack W Fell - One of the best experts on this subject based on the ideXlab platform.
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biodiversity and systematics of basidiomycetous yeasts as determined by large subunit rdna d1 d2 domain sequence analysis
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jack W Fell, Alvaro Fonseca, Gloria Scorzetti, Teun Boekhout, Adele StatzelltallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
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cystofilobasidiales a new order of basidiomycetous yeasts
International Journal of Systematic and Evolutionary Microbiology, 1999Co-Authors: Jack W Fell, H J Roeijmans, Teun BoekhoutAbstract:The order Cystofilobasidiales is described for teleomorphic basidiomycetous yeasts with holobasidia and teliospores. Their septa have dolipores, but lack parenthesomes. D-Glucuronate, nitrate and nitrite are assimilated and myoinositol is usually assimilated. Coenzyme Q has 8 or 10 isoprenologues. 25S and 18S rDNA sequence analysis indicates a monophyletic branch within the Tremellomycetidae of the Hymenomycetes. Cystofilobasidium is the type genus.
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Systematic placement of the basidiomycetous yeast Cystofilobasidium lari-marini comb. nov. as predicted by rRNA nucleotide sequence analysis.
Antonie van Leeuwenhoek, 1992Co-Authors: Jack W Fell, Adele Statzell-tallmanAbstract:Based on similarities in basidial morphology and nucleotide sequences of the V3 variable region in the large sub-unit ribosomal RNA, the yeastLeucosporidium lari-marini is considered phylogenetically related to the genusCystofilobasidium. Therefore the new combinationCystofilobasidium lari-marini is proposed.
Eulogio J. Bedmar - One of the best experts on this subject based on the ideXlab platform.
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Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
Bioresource technology, 2019Co-Authors: Germán Tortosa, Fernando Torralbo, Paula Maza-márquez, Elisabet Aranda, Concepción Calvo, Carmen González-murua, Eulogio J. BedmarAbstract:Abstract Metagenomic and transcriptomic techniques applied to composting could increase our understanding of the overall microbial ecology and could help us to optimise operational conditions which are directly related with economic interest. In this study, the fungal diversity and abundance of two-phase olive mill waste (“alperujo”) composting was studied using Illumina MiSeq sequencing and quantitative PCR, respectively. The results showed an increase of the fungal diversity during the process, with Ascomycota being the predominant phylum. Penicillium was the main genera identified at the mesophilic and maturation phases, with Debaryomyces and Sarocladium at the thermophilic phase, respectively. The fungal abundance was increased during composting, which confirms their important role during thermophilic and maturation phases. Some Basidiomycota showed an increased during the process, which showed a positive correlation with the humification parameters. According to that, the genus Cystofilobasidium could be used as a potential fungal biomarker to assess alperujo compost maturation.
Noboru Tomizuka - One of the best experts on this subject based on the ideXlab platform.
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isolation and transcriptional regulation of the fructose 1 6 bisphosphate aldolase gene fbal in the psychrophilic yeast Cystofilobasidium capitatum
Food Preservation Science, 2007Co-Authors: Shuki Fujimura, Tomoyuki Nakagawa, Toshinori Nagaoka, Tatsuro Miyaji, Masataka Uchino, Takashi Ito, Takao Myoda, Katsumi Takano, Noboru TomizukaAbstract:In this study, we report the primary structure of fructose 1, 6-bisphosphate aldolase encoded by Cystofilobasidium capitatum FBAl (CcFBAl) and the regulation of its gene expression. CcFBAl consists of a 1, 080 bp ORF corresponding to a protein of 360 amino acid residues, and the calculated molecular weight of CcFbalp is 39, 626 Da. The CcFbalp has both aldolase class-II signatures 1 and 2. CcFBAl is expressed at the same level during growth on several different carbon sources, although expression level may be slightly higher on glucose. Transcription of CcFBAl is not influenced by temperature or existence of oxygen, although it was reduced during the stationary phase. We show that CcFbalp is a constitutive enzyme in C. capitatum, and suggest that FBAl could be used as a marker for the detection of pectinolytic psychrophilic yeasts using the PCR method.
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a cold active pectin lyase from the psychrophilic and basidiomycetous yeast Cystofilobasidium capitatum strain ppy 1
Biotechnology and Applied Biochemistry, 2005Co-Authors: Tomoyuki Nakagawa, Toshinori Nagaoka, Tatsuro Miyaji, Noboru TomizukaAbstract:In the present study we purified a cold-active PNL (pectin lyase) from the extracellular fraction of the PPY (pectinolytic and psychrophilic yeast) Cystofilobasidium capitatum strain PPY-1. The purified PNL has a molecular mass of approx. 42 kDa, and its N-terminal amino acid sequence is ATGVTGSAYGFATGTTGGGSATPAY, which exhibits 72% identity with that of PNL F from Aspergillus niger. The purified PNL exhibited high activity at 10 degrees C, although its optimum temperature was 40 degrees C. Moreover, Km and Vmax for pectin as a substrate were found to have values 36.6 mg/ml and 3000 units/mg respectively. These findings may indicate that this enzyme from strain PPY-1 is a cold-active PNL that is able to degrade pectin compounds at low temperature.
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cold active polygalacturonase from psychrophilic basidiomycetous yeast Cystofilobasidium capitatum strain ppy 1
Bioscience Biotechnology and Biochemistry, 2005Co-Authors: Tomoyuki Nakagawa, Toshinori Nagaoka, Tatsuro Miyaji, Noboru TomizukaAbstract:We purified and characterized a cold-active polygalacturonase (PG) from the extracellular fraction of Cystofilobasidium capitatum strain PPY-1. The purified PG from strain PPY-1 has a molecular mass of about 44 kDa, and exhibited high activity at 0 °C, although its optimum temperature was 45 °C. Although the K m value for polygalacturonate as a substrate at 45 °C was found to be 11.2 mg/ml, it decreased gradually with decreasing temperature, and it was 0.66 mg/ml at 0 °C. Moreover, its cleavage pattern was of the endo-type. These findings might indicate that PG from strain PPY-1 is a novel type of cold-active endo-PG that is able to degrade pectin compounds at low temperatures.
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cold active pectinolytic activity of psychrophilic basidiomycetous yeast Cystofilobasidium capitatum strain ppy 1
Journal of Bioscience and Bioengineering, 2002Co-Authors: Tomoyuki Nakagawa, Tatsuro Miyaji, Kaichiro Yamada, Noboru TomizukaAbstract:A pectinolytic and psychrophilic yeast was isolated from soil from Abashiri, Hokkaido, Japan. The phenotype and sequencing of the 28S rDNA of the isolated strain (PPY-1) indicated a taxonomic affiliation to the basidiomycetous yeast Cystofilobasidium capitatum. C. capitatum strain PPY-1 was able to grow on two pectic compounds, polygalacturonate and pectin, at below 5°C. Moreover, the extracellular fraction of the strain exhibited pectin methylesterase, pectin lyase and polygalacturonase activities at 5°C. Thus strain PPY-1 may produce novel enzymes that are able to degrade pectin at low temperature, although the strain has isozymes of these enzymes.
José Paulo Sampaio - One of the best experts on this subject based on the ideXlab platform.
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Cystofilobasidium intermedium sp nov and Cystofilobasidium alribaticum f a sp nov isolated from mediterranean forest soils
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Ana Pontes, Andrey Yurkov, Oliver Rohl, Claudia Carvalho, Cristina Maldonado, José Paulo SampaioAbstract:Multiple isolates belonging to the basidiomycetous genus Cystofilobasidium were obtained from forest soils in Serra da Arrabida Natural Park in Portugal. Phylogenetic analyses employing concatenated sequences of the D1/D2 domain and ITS region support the recognition of two novel species: Cystofilobasidium alribaticum f.a., sp. nov. (type strain CBS 14164T = PYCC 6956T = DSM 101473T) and Cystofilobasidium intermedium sp. nov. (type strain CBS 14089T = PYCC 6856T = DSM 101474T). Whereas C. alribaticum f. a. sp. nov. does not form hyphae, even when different strains are crossed, C. intermedium sp. nov. is self-fertile and forms mycelium with teliospores that upon germination give rise to slender basidia. The most remarkable physiological trait of the two novel species is their ability to grow at 35 °C, a property not observed for remaining species of the genus.
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Cystofilobasidium oberwinkler bandoni 1983
The Yeasts (Fifth Edition), 2011Co-Authors: José Paulo SampaioAbstract:Publisher Summary This chapter discusses the genus Cystofilobasidium. It provides a systematic discussion of the species and concludes with comments on the genus. The diagnosis of the genus is done on the basis of asexual, sexual, physiology and biochemistry, and phylogenetic placement. In asexual reproduction yeast cells are ovoid or ellipsoid. Asexual reproduction is predominantly by polar budding. Poorly developed pseudohyphae may be present in some species. Ballistoconidia are absent. The cultures have an orange to pinkish color that darkens upon aging, but nonpigmented variants are known in some species. Pigmentation is due to the presence of carotenoid pigments. In sexual reproduction species may be self-sterile (heterothallic) or self-fertile (homothallic). The self-sterile compatibility systems are tetrapolar or bipolar. Depending on the species, clamp connections are present or absent. The septal pore is a dolipore without parenthesomes. Teliospores are formed, which are spherical and dark pigmented, and upon germination form slender, tubular or short, nonseptate basidia with terminal and sessile basidiospores that germinate by budding.
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Levaduras del Río Agrio y El Lago Caviahue, un ambiente acuático ácido de origen volcánico (Neuquén, Argentina) Yeasts from the Rio Agrio and Caviahue Lake, an acidic aquatic environment of volcanic origin (Neuquén, Argentina)
Sociedad Argentina de Botánica, 2006Co-Authors: Gabriel Russo, Diego Libkind, José Paulo Sampaio, Maria Van BroockAbstract:Se estudió la biodiversidad de levaduras presente en el ambiente acuático ácido del Río Agrio y el Lago Caviahue. En todas las muestras de agua analizadas se encontraron levaduras en concentraciones variables (25 - 1192 UFC L-1). Fueron aisladas un total de 202 cepas de levaduras, las cuales en base a estudios morfológicos y fisiológicos fueron asignadas a cinco géneros: Candida, Cryptococcus, Cystofilobasidium, Rhodotorula y Sporobolomyces. Los géneros Cryptococcus y Rhodotorula fueron los más abundantes, correspondiendo ambos al 98% del total de las levaduras aisladas. Asimismo, los resultados de las pruebas antes mencionadas sugieren la existencia de al menos dieciocho especies. Este trabajo representa el primer registro cuantitativo y cualitativo de levaduras de un ambiente acuático ácido de origen natural de la Argentina.Yeast biodiversity at the acid aquatic environment of the Agrio River and Caviahue Lake was studied. All water samples analyzed contained yeasts in variable concentrations (25 - 1192 UFC L-1). 202 yeasts strains were isolated and on the basis of morphological and physiological studies were assigned to five genera: Candida, Cryptococcus, Cystofilobasidium, Rhodotorula and Sporobolomyces. Cryptococcus and Rhodotorula were the most abundant genera, comprising the 98% of the isolated yeast strains. The results of the tests mentioned above suggested the existence of at least eighteen different yeast species. This work provides the first quantitative and qualitative data on yeasts from an aquatic acid environment of natural origin in Argentina
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Taxonomic studies on the genus Cystofilobasidium: Description of Cystofilobasidium ferigula sp. nov. and clarification of the status of Cystofilobasidium lari-marini
International Journal of Systematic and Evolutionary Microbiology, 2001Co-Authors: José Paulo Sampaio, Mário Gadanho, Robert BauerAbstract:A new species of the genus Cystofilobasidium is described as Cystofilobasidium ferigula sp. nov. The new taxon represents the teleomorphic stage of Cryptococcus ferigula and was obtained in mating experiments using three strains deposited in the Portuguese Yeast Culture Collection (mating types A1) and a recent isolate (mating type A2). Cystofilobasidium ferigula is characterized using an integrated approach encompassing morphological studies, investigation of the ultrastructure of the septal pore, a comparative study of physiological traits, determination of the DNA base composition, DNA reassociation experiments and PCR fingerprinting. During the course of this study, a close similarity of microsatellite-primed PCR fingerprints was detected between Cystofilobasidium lari-marini and Cystofilobasidium capitatum. DNA-DNA reassociation experiments gave high homology values, which indicates that Cystofilobasidium lari-marini must be regarded as a synonym of Cystofilobasidium capitatum.
Germán Tortosa - One of the best experts on this subject based on the ideXlab platform.
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Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
Bioresource technology, 2019Co-Authors: Germán Tortosa, Fernando Torralbo, Paula Maza-márquez, Elisabet Aranda, Concepción Calvo, Carmen González-murua, Eulogio J. BedmarAbstract:Abstract Metagenomic and transcriptomic techniques applied to composting could increase our understanding of the overall microbial ecology and could help us to optimise operational conditions which are directly related with economic interest. In this study, the fungal diversity and abundance of two-phase olive mill waste (“alperujo”) composting was studied using Illumina MiSeq sequencing and quantitative PCR, respectively. The results showed an increase of the fungal diversity during the process, with Ascomycota being the predominant phylum. Penicillium was the main genera identified at the mesophilic and maturation phases, with Debaryomyces and Sarocladium at the thermophilic phase, respectively. The fungal abundance was increased during composting, which confirms their important role during thermophilic and maturation phases. Some Basidiomycota showed an increased during the process, which showed a positive correlation with the humification parameters. According to that, the genus Cystofilobasidium could be used as a potential fungal biomarker to assess alperujo compost maturation.
