The Experts below are selected from a list of 29655 Experts worldwide ranked by ideXlab platform
Yuanyuan Zhang - One of the best experts on this subject based on the ideXlab platform.
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Myogenic differentiation of human bone marrow mesenchymal stem cells on a 3D nano fibrous scaffold for bladder tissue engineering
2016Co-Authors: Hong Tian, Shantaram Bharadwaj, Peter X, Anthony Atala, Ph. D, Yan Liu, Yuanyuan ZhangAbstract:Bone marrow mesenchymal stem cells (BMSCs) are capable of differentiating into multiple cell types, providing an alternative cell source for cell-based therapy and tissue engineering. Simultaneous differentiation of human BMSCs into smooth muscle cells (SMCs) and urothelium would be beneficial for clinical applications in bladder regeneration for patients with bladder exstrophy or cancer who need cystoplasty. We investigated the ability of human BMSCs to differentiate toward both SMCs and urothelium with cocultured or conditioned media and analyzed growth factors from a coculture system. After being cocultured with urothelium or cultured using urothelium-derived conditioned medium, human BMSCs expressed urothelium-specific genes and proteins: uroplakin-Ia, Cytokeratin-7, and Cytokeratin-13. When cocultured with SMCs or cultured in SMC-conditioned medium, human BMSCs expressed SMC-specific genes and proteins: desmin and myosin. Several growth factors (hepatocyte growth factor, platelet-derived growth factor-homodimer polypeptide of B chain (BB), transforming growth factor-b1, and vascular endothelial growth factor) were detected in the SMC cocultured media and in the urothelium cocultured media (epidermal growth factor, platelet-derived growth factor-BB, transforming growth factor-b1, and vascular endothelial growth factor). BMSC–scaffold constructs significantly improved cell con-tractility after myogenic differentiation. In vivo-grafted cells displayed significant matrix infiltration and ex-pressed SMC-specific markers in the nanofibrous poly-l-lactic acid scaffolds. In conclusion, smooth muscle- and urothelium-like cells derived from human BMSCs provide an alternative cell source for potential use in bladder tissue engineering
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differentiation of human bone marrow mesenchymal stem cells into bladder cells potential for urological tissue engineering
Tissue Engineering Part A, 2010Co-Authors: Hong Tian, Shantaram Bharadwaj, Peter X, Anthony Atala, Yuanyuan ZhangAbstract:Bone marrow mesenchymal stem cells (BMSCs) are capable of differentiating into multiple cell types, providing an alternative cell source for cell-based therapy and tissue engineering. Simultaneous differentiation of human BMSCs into smooth muscle cells (SMCs) and urothelium would be beneficial for clinical applications in bladder regeneration for patients with bladder exstrophy or cancer who need cystoplasty. We investigated the ability of human BMSCs to differentiate toward both SMCs and urothelium with cocultured or conditioned media and analyzed growth factors from a coculture system. After being cocultured with urothelium or cultured using urothelium-derived conditioned medium, human BMSCs expressed urothelium-specific genes and proteins: uroplakin-Ia, Cytokeratin-7, and Cytokeratin-13. When cocultured with SMCs or cultured in SMC-conditioned medium, human BMSCs expressed SMC-specific genes and proteins: desmin and myosin. Several growth factors (hepatocyte growth factor, platelet-derived growth factor-homodimer polypeptide of B chain (BB), transforming growth factor-β1, and vascular endothelial growth factor) were detected in the SMC cocultured media and in the urothelium cocultured media (epidermal growth factor, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor). BMSC–scaffold constructs significantly improved cell contractility after myogenic differentiation. In vivo-grafted cells displayed significant matrix infiltration and expressed SMC-specific markers in the nanofibrous poly-l-lactic acid scaffolds. In conclusion, smooth muscle- and urothelium-like cells derived from human BMSCs provide an alternative cell source for potential use in bladder tissue engineering.
Hong Tian - One of the best experts on this subject based on the ideXlab platform.
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Myogenic differentiation of human bone marrow mesenchymal stem cells on a 3D nano fibrous scaffold for bladder tissue engineering
2016Co-Authors: Hong Tian, Shantaram Bharadwaj, Peter X, Anthony Atala, Ph. D, Yan Liu, Yuanyuan ZhangAbstract:Bone marrow mesenchymal stem cells (BMSCs) are capable of differentiating into multiple cell types, providing an alternative cell source for cell-based therapy and tissue engineering. Simultaneous differentiation of human BMSCs into smooth muscle cells (SMCs) and urothelium would be beneficial for clinical applications in bladder regeneration for patients with bladder exstrophy or cancer who need cystoplasty. We investigated the ability of human BMSCs to differentiate toward both SMCs and urothelium with cocultured or conditioned media and analyzed growth factors from a coculture system. After being cocultured with urothelium or cultured using urothelium-derived conditioned medium, human BMSCs expressed urothelium-specific genes and proteins: uroplakin-Ia, Cytokeratin-7, and Cytokeratin-13. When cocultured with SMCs or cultured in SMC-conditioned medium, human BMSCs expressed SMC-specific genes and proteins: desmin and myosin. Several growth factors (hepatocyte growth factor, platelet-derived growth factor-homodimer polypeptide of B chain (BB), transforming growth factor-b1, and vascular endothelial growth factor) were detected in the SMC cocultured media and in the urothelium cocultured media (epidermal growth factor, platelet-derived growth factor-BB, transforming growth factor-b1, and vascular endothelial growth factor). BMSC–scaffold constructs significantly improved cell con-tractility after myogenic differentiation. In vivo-grafted cells displayed significant matrix infiltration and ex-pressed SMC-specific markers in the nanofibrous poly-l-lactic acid scaffolds. In conclusion, smooth muscle- and urothelium-like cells derived from human BMSCs provide an alternative cell source for potential use in bladder tissue engineering
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differentiation of human bone marrow mesenchymal stem cells into bladder cells potential for urological tissue engineering
Tissue Engineering Part A, 2010Co-Authors: Hong Tian, Shantaram Bharadwaj, Peter X, Anthony Atala, Yuanyuan ZhangAbstract:Bone marrow mesenchymal stem cells (BMSCs) are capable of differentiating into multiple cell types, providing an alternative cell source for cell-based therapy and tissue engineering. Simultaneous differentiation of human BMSCs into smooth muscle cells (SMCs) and urothelium would be beneficial for clinical applications in bladder regeneration for patients with bladder exstrophy or cancer who need cystoplasty. We investigated the ability of human BMSCs to differentiate toward both SMCs and urothelium with cocultured or conditioned media and analyzed growth factors from a coculture system. After being cocultured with urothelium or cultured using urothelium-derived conditioned medium, human BMSCs expressed urothelium-specific genes and proteins: uroplakin-Ia, Cytokeratin-7, and Cytokeratin-13. When cocultured with SMCs or cultured in SMC-conditioned medium, human BMSCs expressed SMC-specific genes and proteins: desmin and myosin. Several growth factors (hepatocyte growth factor, platelet-derived growth factor-homodimer polypeptide of B chain (BB), transforming growth factor-β1, and vascular endothelial growth factor) were detected in the SMC cocultured media and in the urothelium cocultured media (epidermal growth factor, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor). BMSC–scaffold constructs significantly improved cell contractility after myogenic differentiation. In vivo-grafted cells displayed significant matrix infiltration and expressed SMC-specific markers in the nanofibrous poly-l-lactic acid scaffolds. In conclusion, smooth muscle- and urothelium-like cells derived from human BMSCs provide an alternative cell source for potential use in bladder tissue engineering.
Amna Riaz Khan - One of the best experts on this subject based on the ideXlab platform.
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diagnostic utility of Cytokeratin 13 and Cytokeratin 17 in oral epithelial dysplasia and oral squamous cell carcinoma
Asian Pacific Journal of Cancer Biology, 2020Co-Authors: Maryam Nazir Kiani, Muhammad Asif, Fakeha Meraj Ansari, Nighat Ara, Muhammad Ishaque, Amna Riaz KhanAbstract:Objective: To determine the immunohistochemical expression of CK 13 and CK 17 in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma. Methods: A total of 170 cases were retrieved from record files of Histopathology Department, to conduct a cross sectional study at Armed Forces Institute of Pathology, Rawalpindi, over a period of one year from June 2018 to June 2019 along with their formalin-fixed, paraffin embedded blocks comprising 85 cases of each oral squamous cell carcinoma and oral epithelial dysplasia. Blocks were trimmed and cut into very thin sections of 5 microns using microtome and mounted on slides. Tissue mounted slides were stained with routine haematoxylin and eosin followed by immunohistochemical staining of Cytokeratin 13 and Cytokeratin 17. New histological diagnosis of each case was made. Mean and standard deviation were calculated for quantitative variables. Frequency and percentages were calculated for qualitative variables. Chi-square test was employed to assess the significance of difference. The P-value <0.05 was considered significant. Results: In this study, 101 (59.4%) male and 69 (40.6%) female patients with the mean age of 60.63 ± 13.814 (mean ± SD) were collected and buccal mucosa was the most common site of presentation. In a total of 170 cases, 34 (20%) cases showed positive expression of Cytokeratin 13 whereas 136 (80%) cases showed negative expression of Cytokeratin 13. In comparison, out of 170 cases, 133 (78.2%) cases showed positive expression of Cytokeratin 17 whereas 37 (21.8%) cases showed negative expression of Cytokeratin 17. The P-value was found to be < 0.001 and 0.001 for expression of Cytokeratin 13 and Cytokeratin 17 respectively. Conclusion: Opposite expression of Cytokeratin 13 and Cytokeratin 17 was seen in this study, in the form of loss of Cytokeratin 13 and over expression of Cytokeratin 17 with increase in the degree of dysplasia and invasive carcinoma. Correct assessment, diagnosis and management with the help of these markers can lead to early diagnosis and favorable treatment outcome.
Akira Katakura - One of the best experts on this subject based on the ideXlab platform.
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Cytokeratin 13 Cytokeratin 17 ki 67 and p53 expression in upper layers of epithelial dysplasia surrounding tongue squamous cell carcinoma
The Bulletin of Tokyo Dental College, 2015Co-Authors: Akiko Matsuhira, Kazumichi Sato, Yoichi Tanaka, Sunaki Noguchi, Gou Yamamoto, Kenji Mishima, Akira KatakuraAbstract:Early detection of oral squamous cell carcinoma (OSCC) improves its prognosis and aids in selecting the appropriate treatment, which may also have a positive effect on quality of life. Early detection, therefore, is an important issue in the treatment of this disease. The purpose of this study was to investigate expression of Cytokeratin 13 (CK13), CK17, Ki-67 and p53 as potential markers of tongue SCC. Five areas in 12 specimens were examined: the upper and lower layers of normal epithelium; those of dysplastic epithelial tissue surrounding the cancerous lesion; and the lesion itself. Strong expression of each of the following mRNAs and proteins was observed; CK13 in upper layers of normal epithelium; Ki-67 and p53 in lower layers of normal epithelium; CK13 and CK17 in upper layer of epithelial dysplasia; and CK17, Ki-67, and p53 in lower layer of epithelial dysplasia and cancerous lesions. These results indicate that the characteristic pattern of expression of CK13 and CK17 differs between normal and dysplastic oral epithelium. Oral epithelial dysplasia adjacent to OSCC has high malignant potential, and is similar to early-stage OSCC. This suggests that evaluation of these markers could be a useful secondary procedure for improving detection of early-stage OSCC.
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evaluation of superficial oral squamous cell malignancy based on morphometry and immunoexpression of Cytokeratin 13 and Cytokeratin 17
Acta Cytologica, 2014Co-Authors: Mitsumasa Yamashina, Kazumichi Sato, Morio Tonogi, Yoichi Tanaka, Gen-yuki Yamane, Akira KatakuraAbstract:Objective: Evaluation of combined morphometry and immunoexpression of Cytokeratin 13 (CK13) and Cytokeratin 17 (CK17) for cytological identification of superficial oral squamous cells. Study Design: Smears from 11 tongue squamous cell carcinoma patients were processed by liquid-based cytology, stained via the Papanicolaou method and divided into multiple specimens by cell transfer. Morphometric indices, including nuclear area, nuclear perimeter, nuclear circular rate, largest-to-smallest dimension ratio of the nucleus and nucleocytoplasmic ratio, were measured using a computerized analysis system. CK13 and CK17 were detected by immunostaining. Morphometric values were compared between cell populations with distinct staining and immunoexpression patterns. Results: Most orange G-stained superficial cells were negative for CK13 (99.4%) and CK17 (98.6%). For light green-stained superficial cells, loss of CK13 was associated with greater cellular atypia in the nuclear area, nuclear perimeter and nucleocytoplasmic ratio (p Conclusion: Immunoexpression of CK13 and CK17 in light green-stained superficial cells was associated with more severe morphological atypia. Combined morphometry and immunoexpression of CK13 and CK17 might be useful for cytological diagnosis of this cell population.
Gen-yuki Yamane - One of the best experts on this subject based on the ideXlab platform.
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expression of Cytokeratin 13 14 17 and 19 in 4 nitroquinoline 1 oxide induced oral carcinogenesis in rat
The Bulletin of Tokyo Dental College, 2016Co-Authors: Tomoyoshi Saitoh, Kazumichi Sato, Morio Tonogi, Yoichi Tanaka, Gen-yuki YamaneAbstract:The management of epithelial dysplastic spread around an oral squamous cell carci-noma is very important, particularly intraoperatively. Both Cytokeratin (CK) 14 and CK19 are believed to be involved in the development of precancerous lesions, and their expression profiles are quite specific in these and early cancer lesions. Here, expression of CK13, 14, 17, and 19 was investigated in a rat model of 4-nitroquinoline-1-oxide-induced tongue cancer during a series of carcinogenetic processes to determine their value in assessing the features of epithelial dysplastic spread around a cancer. Based on tissue conditions, the results showed that expression levels of CK13 and 14 decreased in the order of no change, dysplasia, and cancer, whereas those of CK17 and 19 increased in the same order. Expression of CK13 showed a significant difference among no change, dysplasia, and cancer. This indicates that comparing the immunohistochemical staining profiles of CKs, especially CK13, could help in assessing the characteristics of epithelial dysplastic spread around a cancer.
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evaluation of superficial oral squamous cell malignancy based on morphometry and immunoexpression of Cytokeratin 13 and Cytokeratin 17
Acta Cytologica, 2014Co-Authors: Mitsumasa Yamashina, Kazumichi Sato, Morio Tonogi, Yoichi Tanaka, Gen-yuki Yamane, Akira KatakuraAbstract:Objective: Evaluation of combined morphometry and immunoexpression of Cytokeratin 13 (CK13) and Cytokeratin 17 (CK17) for cytological identification of superficial oral squamous cells. Study Design: Smears from 11 tongue squamous cell carcinoma patients were processed by liquid-based cytology, stained via the Papanicolaou method and divided into multiple specimens by cell transfer. Morphometric indices, including nuclear area, nuclear perimeter, nuclear circular rate, largest-to-smallest dimension ratio of the nucleus and nucleocytoplasmic ratio, were measured using a computerized analysis system. CK13 and CK17 were detected by immunostaining. Morphometric values were compared between cell populations with distinct staining and immunoexpression patterns. Results: Most orange G-stained superficial cells were negative for CK13 (99.4%) and CK17 (98.6%). For light green-stained superficial cells, loss of CK13 was associated with greater cellular atypia in the nuclear area, nuclear perimeter and nucleocytoplasmic ratio (p Conclusion: Immunoexpression of CK13 and CK17 in light green-stained superficial cells was associated with more severe morphological atypia. Combined morphometry and immunoexpression of CK13 and CK17 might be useful for cytological diagnosis of this cell population.
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Expression of Cytokeratin 13 and 17 in tongue squamous cell carcinoma and epithelial dysplasia
Asian Journal of Oral and Maxillofacial Surgery, 2011Co-Authors: Sunaki Noguchi, Kazumichi Sato, Morio Tonogi, Yoichi Tanaka, Gou Yamamoto, Tetsuhiko Tachikawa, Gen-yuki YamaneAbstract:Abstract Objective We have searched for the factor to diagnose malignant potential of epithelial dysplasia. Recent studies have suggested that determination of the expression of Cytokeratin 13 and 17 would be useful for the early diagnosis of oral squamous cell carcinoma. In this study, we focused on the diagnosis based on the malignant potential of epithelial dysplasia and evaluated the feasibility of combined assessment of Cytokeratin 13 and 17. Patients and methods The expression of Cytokeratin 13 and 17 observed in 8 patients with clinically diagnosed T1 or T2 tongue squamous cell carcinoma was analyzed using real-time PCR and immunohistochemical staining of resected specimens. Areas in each specimen were identified as Normal, Dysplasia and Cancer. Results Real-time PCR showed that the expression of Cytokeratin 13 decreased beginning with Dysplasia, whereas the expression of Cytokeratin 17 increased beginning with Dysplasia. Immunohistochemical staining revealed that Cytokeratin 13-positive cells decreased beginning with Dysplasia, while there were almost no Cytokeratin 13-positive cells in Cancer. Conversely, Cytokeratin 17-positive cells increased beginning with Dysplasia, and almost all cells were Cytokeratin 17-positive in Cancer. These results thus indicate that the expression of Cytokeratin 13 and 17 is associated with carcinogenesis in epithelial dysplasia. Conclusions The findings of this study indicate that assessment of the expression of Cytokeratin 13 and 17 might be useful in the diagnosis of malignant potential in epithelial dysplasia. Moreover, evaluating changes in the expression of Cytokeratin 13 and 17 could be effective in evaluating surgical margins.
