D-Glucosamine

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Isao Nagaoka - One of the best experts on this subject based on the ideXlab platform.

  • Recent aspects of the anti-inflammatory actions of glucosamine
    Carbohydrate Polymers, 2011
    Co-Authors: Isao Nagaoka, Shin Yomogida, Mamoru Igarashi, Y. Ju, Koji Sakamoto
    Abstract:

    Glucosamine, a naturally occurring amino monosaccharide, is present in the connective and cartilage tissues as a component of glycosaminoglycans. Thus, glucosamine has been widely used to treat osteoarthritis, a joint disease characterized by cartilage degeneration, in humans. In addition, glucosamine is expected to exert an anti-inflammatory action, since glucosamine suppresses inflammatory cell activation. To further extend the anti-inflammatory actions of glucosamine, we investigated the effects of glucosamine on synovial cells, endothelial cells and intestinal epithelial cells using in vitro and in vivo systems. Firstly, glucosamine suppressed the IL-1β-induced activation of synovial cells in vitro. Furthermore, glucosamine administration repressed synovial cell hyperplasia, cartilage destruction and inflammatory cell infiltration in rat adjuvant arthritis. Secondary, glucosamine suppressed the TNF-α-induced activation of intestinal epithelial cell HT-29 in vitro. In addition, glucosamine administration improved the clinical symptoms, and colonic inflammation and tissue injury in dextran sulfate sodium-induced colitis in rats. Finally, glucosamine suppressed the TNF-α-induced activation of endothelial cells in vitro. Moreover, glucosamine administration repressed the formation of atherosclerotic lesion and infiltration of inflammatory cells into the lesion in spontaneously hyperlipidemic mice B6 KOR Aposhl. Together these observations support the idea that glucosamine can function as not only a chondroprotective agent but also an anti-inflammatory molecule in the body.

  • Inhibitory action of glucosamine on platelet activation in guinea pigs.
    Inflammation Research, 2005
    Co-Authors: J. F. Lu-suguro, Koji Sakamoto, Isao Nagaoka
    Abstract:

    Objective: Glucosamine, a naturally occurring amino monosaccharide, has been used to treat or prevent osteoarthritis in humans. Recently, we have revealed that glucosamine inhibits platelet activation in vitro. However, the effect of in vivo administration of glucosamine has not yet been clarified. In this study, we administered glucosamine orally to guinea pigs and examined its effects on platelet functions.

  • Inhibitory action of glucosamine on platelet activation in guinea pigs
    Inflammation Research, 2005
    Co-Authors: J. F. Lu-suguro, Koji Sakamoto, Isao Nagaoka
    Abstract:

    Objective: Glucosamine, a naturally occurring amino monosaccharide, has been used to treat or prevent osteoarthritis in humans. Recently, we have revealed that glucosamine inhibits platelet activation in vitro . However, the effect of in vivo administration of glucosamine has not yet been clarified. In this study, we administered glucosamine orally to guinea pigs and examined its effects on platelet functions. Materials and methods: Glucosamine hydrochloride solution (0.5%, 5 mg/ml) was administered orally to guinea pigs ad libitum for 22 days, and platelet rich plasma was collected to evaluate platelet functions in vitro . Guinea pigs received an average of 400 mg glucosamine/animal/day. Results: Glucosamine-administration suppressed platelet aggregation in response to ADP by 51% ( p  

  • Preventive actions of a high dose of glucosamine on adjuvant arthritis in rats
    Inflammation Research, 2005
    Co-Authors: S Suguro, S. Hirano, K Sakamoto, Isao Nagaoka
    Abstract:

    Objective: Glucosamine, a naturally occurring amino monosaccharide has been used to treat or prevent osteoarthritis in humans. In this study, we evaluated the effect of glucosamine on rat adjuvant arthritis, a model of rheumatoid arthritis. Materials and methods: Adjuvant arthritis was induced in male Wistar rats by injection of Freund’s complete adjuvant (FCA) into the right hind paw, and 300 mg/kg of glucosamine, an extra-dose compared with a regular dose for osteoarthritis patients (1.5 g/day, approximately 25 mg/kg), was orally administered once a day to the arthritic rats for 22 days. Results: Glucosamine significantly suppressed the increase in arthritis score ( p < 0.05) after day 10 of adjuvant injection, and inhibited the swelling of FCA-injected right and -uninjected left hind paws ( p < 0.01) after day 18. In addition, histopathological examination of the arthritic joints revealed that glucosamine suppressed synovial hyperplasia, cartilage destruction and inflammatory cell infiltration. Furthermore, glucosamine reduced the production of nitric oxide and prostaglandin E_2 in plasma ( p < 0.05). Conclusions: These observations suggest that glucosamine is able to suppress the progression of adjuvant arthritis in rats. Glucosamine may be expected as a novel anti-inflammatory agent for treatment of rheumatoid arthritis.

  • Glucosamine, a naturally occurring amino monosaccharide, suppresses the ADP-mediated platelet activation in humans
    Inflammation Research, 2004
    Co-Authors: S Suguro, Y. Tsutsumi-ishii, Kazuhisa Iwabuchi, Koji Sakamoto, Isao Nagaoka
    Abstract:

    Objective: To evaluate the anti-thrombotic action of glucosamine, a naturally occurring amino monosaccharide, platelets were stimulated with ADP in the presence of glucosamine, and its effects on platelet functions were examined. Materials and Methods: Human platelet-rich plasma was stimulated with 2.5 μM ADP in the presence of glucosamine (0.01 ~ 1 mM) or other aminosugars (N-acetyl-glucosamine, galactosamine or N-acetyl-galactosamine, 1 mM), and platelet aggregation was monitored. Furthermore, the effects of glucosamine on the thromboxane A2 production, release of granule contents, intracellular calcium mobilization and phosphorylation of Syk (a 72 kD protein tyrosine kinase) were evaluated following ADP-stimulation. In addition, the binding of [^3H] ADP to its receptors was examined. Results: Glucosamine (>0.01 mM) dose-dependently suppressed platelet aggregation in response to ADP ( p < 0.05), whereas N-acetyl-glucosamine, galactosamine or N-acetyl-galactosamine (1 mM) did not affect the ADP-induced platelet aggregation. Furthermore, glucosamine (>0.1 mM) inhibited the extracellular release of granule contents (ATP and platelet factor 4) and production of thromboxane A_2 from ADP-stimulated platelets ( p < 0.05). Moreover, glucosamine significantly repressed the intracellular calcium mobilization at >0.1 mM and phosphorylation of Syk at >0.01 mM upon ADP-stimulation ( p < 0.05). In addition, glucosamine (>0.1 mM) inhibited the binding of ADP to its receptors ( p < 0.05). Conclusion: Glucosamine is able to suppress platelet aggregation, release of granule constituents, thromboxane A_2 production, calcium mobilization and phosphorylation of Syk possibly via the inhibition of ADP-binding to the receptors. Glucosamine could be expected as a novel anti-platelet agent for thrombotic disorders due to its suppressive actions on platelets.

Koji Sakamoto - One of the best experts on this subject based on the ideXlab platform.

  • Recent aspects of the anti-inflammatory actions of glucosamine
    Carbohydrate Polymers, 2011
    Co-Authors: Isao Nagaoka, Shin Yomogida, Mamoru Igarashi, Y. Ju, Koji Sakamoto
    Abstract:

    Glucosamine, a naturally occurring amino monosaccharide, is present in the connective and cartilage tissues as a component of glycosaminoglycans. Thus, glucosamine has been widely used to treat osteoarthritis, a joint disease characterized by cartilage degeneration, in humans. In addition, glucosamine is expected to exert an anti-inflammatory action, since glucosamine suppresses inflammatory cell activation. To further extend the anti-inflammatory actions of glucosamine, we investigated the effects of glucosamine on synovial cells, endothelial cells and intestinal epithelial cells using in vitro and in vivo systems. Firstly, glucosamine suppressed the IL-1β-induced activation of synovial cells in vitro. Furthermore, glucosamine administration repressed synovial cell hyperplasia, cartilage destruction and inflammatory cell infiltration in rat adjuvant arthritis. Secondary, glucosamine suppressed the TNF-α-induced activation of intestinal epithelial cell HT-29 in vitro. In addition, glucosamine administration improved the clinical symptoms, and colonic inflammation and tissue injury in dextran sulfate sodium-induced colitis in rats. Finally, glucosamine suppressed the TNF-α-induced activation of endothelial cells in vitro. Moreover, glucosamine administration repressed the formation of atherosclerotic lesion and infiltration of inflammatory cells into the lesion in spontaneously hyperlipidemic mice B6 KOR Aposhl. Together these observations support the idea that glucosamine can function as not only a chondroprotective agent but also an anti-inflammatory molecule in the body.

  • Inhibitory action of glucosamine on platelet activation in guinea pigs.
    Inflammation Research, 2005
    Co-Authors: J. F. Lu-suguro, Koji Sakamoto, Isao Nagaoka
    Abstract:

    Objective: Glucosamine, a naturally occurring amino monosaccharide, has been used to treat or prevent osteoarthritis in humans. Recently, we have revealed that glucosamine inhibits platelet activation in vitro. However, the effect of in vivo administration of glucosamine has not yet been clarified. In this study, we administered glucosamine orally to guinea pigs and examined its effects on platelet functions.

  • Inhibitory action of glucosamine on platelet activation in guinea pigs
    Inflammation Research, 2005
    Co-Authors: J. F. Lu-suguro, Koji Sakamoto, Isao Nagaoka
    Abstract:

    Objective: Glucosamine, a naturally occurring amino monosaccharide, has been used to treat or prevent osteoarthritis in humans. Recently, we have revealed that glucosamine inhibits platelet activation in vitro . However, the effect of in vivo administration of glucosamine has not yet been clarified. In this study, we administered glucosamine orally to guinea pigs and examined its effects on platelet functions. Materials and methods: Glucosamine hydrochloride solution (0.5%, 5 mg/ml) was administered orally to guinea pigs ad libitum for 22 days, and platelet rich plasma was collected to evaluate platelet functions in vitro . Guinea pigs received an average of 400 mg glucosamine/animal/day. Results: Glucosamine-administration suppressed platelet aggregation in response to ADP by 51% ( p  

  • Glucosamine, a naturally occurring amino monosaccharide, suppresses the ADP-mediated platelet activation in humans
    Inflammation Research, 2004
    Co-Authors: S Suguro, Y. Tsutsumi-ishii, Kazuhisa Iwabuchi, Koji Sakamoto, Isao Nagaoka
    Abstract:

    Objective: To evaluate the anti-thrombotic action of glucosamine, a naturally occurring amino monosaccharide, platelets were stimulated with ADP in the presence of glucosamine, and its effects on platelet functions were examined. Materials and Methods: Human platelet-rich plasma was stimulated with 2.5 μM ADP in the presence of glucosamine (0.01 ~ 1 mM) or other aminosugars (N-acetyl-glucosamine, galactosamine or N-acetyl-galactosamine, 1 mM), and platelet aggregation was monitored. Furthermore, the effects of glucosamine on the thromboxane A2 production, release of granule contents, intracellular calcium mobilization and phosphorylation of Syk (a 72 kD protein tyrosine kinase) were evaluated following ADP-stimulation. In addition, the binding of [^3H] ADP to its receptors was examined. Results: Glucosamine (>0.01 mM) dose-dependently suppressed platelet aggregation in response to ADP ( p < 0.05), whereas N-acetyl-glucosamine, galactosamine or N-acetyl-galactosamine (1 mM) did not affect the ADP-induced platelet aggregation. Furthermore, glucosamine (>0.1 mM) inhibited the extracellular release of granule contents (ATP and platelet factor 4) and production of thromboxane A_2 from ADP-stimulated platelets ( p < 0.05). Moreover, glucosamine significantly repressed the intracellular calcium mobilization at >0.1 mM and phosphorylation of Syk at >0.01 mM upon ADP-stimulation ( p < 0.05). In addition, glucosamine (>0.1 mM) inhibited the binding of ADP to its receptors ( p < 0.05). Conclusion: Glucosamine is able to suppress platelet aggregation, release of granule constituents, thromboxane A_2 production, calcium mobilization and phosphorylation of Syk possibly via the inhibition of ADP-binding to the receptors. Glucosamine could be expected as a novel anti-platelet agent for thrombotic disorders due to its suppressive actions on platelets.

  • Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils
    Journal of Leukocyte Biology, 2002
    Co-Authors: Koji Sakamoto, Isao Nagaoka
    Abstract:

    : Glucosamine, an amino monosaccharide naturally occurring in the connective and cartilage tissues, contributes to maintaining the strength, flexibility, and elasticity of these tissues. In recent years, glucosamine has been used widely to treat osteoarthritis in humans and animal models. Neutrophils, which usually function as the primary defenders in bacterial infections, are also implicated in the destructive, inflammatory responses in arthritis. In this study, we have evaluated the effects of glucosamine on neutrophil functions using human peripheral blood neutrophils. Glucosamine (0.01-1 mM) dose-dependently suppressed the superoxide anion generation induced by formyl-Met-Leu-Phe (fMLP) or complement-opsonized zymosan and inhibited the phagocytosis of complement-opsonized zymosan or IgG-opsonized latex particles. Furthermore, glucosamine inhibited the release of granule enzyme lysozyme from phagocytosing neutrophils and suppressed neutrophil chemotaxis toward zymosan-activated serum. In addition, glucosamine inhibited fMLP-induced up-regulation of CD11b significantly, polymerization of actin, and phosphorylation of p38 mitogen-activated protein kinase (MAPK). In contrast, N-acetyl-glucosamine, an analogue of glucosamine, did not affect these neutrophil functions (superoxide generation, phagocytosis, granule enzyme release, chemotaxis, CD11b expression, actin polymerization, and p38 MAPK phosphorylation) at the concentrations examined (1-10 mM). Together these observations likely suggest that glucosamine suppresses the neutrophil functions, thereby possibly exhibiting anti-inflammatory actions in arthritis.

C. S. Park - One of the best experts on this subject based on the ideXlab platform.

  • Lactose-egg yolk diluent supplemented with N-acetyl-D-Glucosamine affect acrosome morphology and motility of frozen-thawed boar sperm.
    Animal Reproduction Science, 2002
    Co-Authors: Yoon-sun Yi, Gi-sun Im, C. S. Park
    Abstract:

    Abstract These experiments were carried out to investigate the effect of N -acetyl- d -glucosamine, and to obtain additional information about the effect of orvus es paste (OEP) and egg yolk concentration in the freezing of boar sperm in the maxi-straw. The highest post-thaw acrosomes of normal apical ridge (NAR) and motility were obtained with 0.025 or 0.05% N -acetyl- d -glucosamine concentration in the first diluent. However, there were no effects of N -acetyl- d -glucosamine among the diluents with or without N -acetyl- d -glucosamine at the second dilution. The N -acetyl- d -glucosamine in the first and second diluents was added at room temperatures (20–23 °C) and 5 °C, respectively. It is suggested that the temperature of N -acetyl- d -glucosamine addition is important for the effect of boar sperm protection during freezing and thawing. When the 0.05% N -acetyl- d -glucosamine was supplemented in the first diluent, the optimum final OEP content was 0.5%. The optimum content of egg yolk in the diluent with 0.05% N -acetyl- d -glucosamine concentration was 20% and egg yolk was one of the main cryoprotective agents. In conclusion, we found out that the diluent with 0.025 or 0.05% soluble N -acetyl- d -glucosamine in the first diluent, 0.5% final orvus es paste concentration and 20% egg yolk concentration significantly enhanced NAR acrosomes and motility of boar sperm after freezing and thawing.

  • Lactose-egg yolk diluent supplemented with N-acetyl-D-Glucosamine affect acrosome morphology and motility of frozen-thawed boar sperm.
    Animal reproduction science, 2002
    Co-Authors: Y J Yi, Gi-sun Im, C. S. Park
    Abstract:

    These experiments were carried out to investigate the effect of N-acetyl-D-Glucosamine, and to obtain additional information about the effect of orvus es paste (OEP) and egg yolk concentration in the freezing of boar sperm in the maxi-straw. The highest post-thaw acrosomes of normal apical ridge (NAR) and motility were obtained with 0.025 or 0.05% N-acetyl-D-Glucosamine concentration in the first diluent. However, there were no effects of N-acetyl-D-Glucosamine among the diluents with or without N-acetyl-D-Glucosamine at the second dilution. The N-acetyl-D-Glucosamine in the first and second diluents was added at room temperatures (20-23 degrees C) and 5 degrees C, respectively. It is suggested that the temperature of N-acetyl-D-Glucosamine addition is important for the effect of boar sperm protection during freezing and thawing. When the 0.05% N-acetyl-D-Glucosamine was supplemented in the first diluent, the optimum final OEP content was 0.5%. The optimum content of egg yolk in the diluent with 0.05% N-acetyl-D-Glucosamine concentration was 20% and egg yolk was one of the main cryoprotective agents. In conclusion, we found out that the diluent with 0.025 or 0.05% soluble N-acetyl-D-Glucosamine in the first diluent, 0.5% final orvus es paste concentration and 20% egg yolk concentration significantly enhanced NAR acrosomes and motility of boar sperm after freezing and thawing.

  • Effect of N-acetyl-D-Glucosamine, and glycerol concentration and equilibration time on acrosome morphology and motility of frozen-thawed boar sperm.
    Animal reproduction science, 2002
    Co-Authors: Y J Yi, Y M Cheon, C. S. Park
    Abstract:

    A series of experiments were conducted to determine the effect of N-acetyl-D-Glucosamine, glycerol concentration and equilibration time for the freezing of boar spermatozoa in 5 ml maxi-straws. The optimum final glycerol concentration in the diluent with 0.05% N-acetyl-D-Glucosamine in the first diluent was 2-3% and the optimum glycerol equilibration time was 2-3h. In conclusion, we recommend the first diluent containing 11% lactose hydrate, 20% egg yolk and 0.05% N-acetyl-D-Glucosamine in 100ml distilled water, and the second diluent containing 11% lactose hydrate, 20% egg yolk, 4% glycerol and 1% orvus es paste for the diluents of boar sperm freezing. Also, we found out that 0.05% soluble N-acetyl-D-Glucosamine was the optimum concentration in the first diluent and a concentration of 0.05% soluble N-acetyl-D-Glucosamine significantly enhanced the cryopreservation of boar spermatozoa.

Alan D Grund - One of the best experts on this subject based on the ideXlab platform.

Mingde Deng - One of the best experts on this subject based on the ideXlab platform.