Daidzin

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Jaehwan Lee - One of the best experts on this subject based on the ideXlab platform.

  • Formation of succinyl genistin and succinyl Daidzin by Bacillus species.
    Journal of food science, 2010
    Co-Authors: Chan Uk Park, Min Kyu Jeong, Min Hee Park, Joodong Yeu, Myeong Soo Park, Mi-ja Kim, Seon Min Ahn, Pahn-shick Chang, Jaehwan Lee
    Abstract:

    6''-O-Succinyl-4'-hydroxyisoflavone-7-O-beta-D-glucopyranoside (succinyl-beta-Daidzin) and 6''-O-succinyl-6,4'-dihydroxyisoflavone-7-O-beta-D-glucopyranoside (succinyl-beta-genistin), 2 new isoflavone metabolites, are found in cheonggukjang or natto, traditional soy-based foods fermented with Bacillus species. Standard isoflavones including Daidzin, genistin, daidzein, and genistein, and mixtures of isoflavones extracted from roasted soybeans were added to the medium growing Bacillus subtilis or B. subtilis natto and formation of succinyl-beta-Daidzin and succinyl-beta-genistin were monitored by high-performance liquid chromatography (HPLC). Samples containing Bacillus with Daidzin and genistin produced succinyl-beta-Daidzin and succinyl-beta-genistin, respectively, while those with daidzein and genistein did not produce succinyl derivatives. Daidzin in samples with B. subtilis and B. subtilis natto decreased by 39.7% and 10.7%, respectively, for 4 h incubation while genistin decreased by 66.8% and 17.6%, respectively. Genistein decreased faster than daidzein during incubation with B. subtilis or B. subtilis natto without formation of succinyl derivatives. In the case of mixture of isoflavones, succinyl derivatives increased and beta-glucosides and aglycones of isoflavones decreased significantly for 8 h incubation (P < 0.05).

Bert L. Vallee - One of the best experts on this subject based on the ideXlab platform.

  • Potentiation of the bioavailability of Daidzin by an extract of Radix puerariae.
    Proceedings of the National Academy of Sciences of the United States of America, 1996
    Co-Authors: Wing-ming Keung, Oscar Lazo, Lisa Kunze, Bert L. Vallee
    Abstract:

    Abstract The dose effect of pure Daidzin on the suppression of ethanol intake in Syrian golden hamsters was compared with that of crude Daidzin contained in a methanol extract of Radix puerariae (RP). EC50 values estimated from the graded dose-response curves for pure Daidzin and RP extract Daidzin are 23 and 2.3 mg per hamster per day, respectively. Apparently the antidipsotropic activity of the RP extract cannot be accounted for solely by its Daidzin content (22 mg/g). In addition to Daidzin, six other isoflavones were identified in the RP extract and quantified--namely, puerarin (160 mg per g of extract), genistin (3.7 mg/g), daidzein (2.6 mg/g), daidzein-4',7-diglucoside (1.2 mg/g), genistein (0.2 mg/g), and formononetin (0.16 mg/g). None of these, administered either alone or combined, contributes in any significant way to the antidipsotropic activity of the extract. Plasma Daidzin concentration-time curves determined in hamsters administered various doses of pure Daidzin or RP extract by i.p.injection indicate that the crude extract Daidzin has approximately 10 times greater bioavailability than the pure compound. Reconstruction of the dose-response effects for pure and crude Daidzin using bioavailable Daidzin rather than administered dose gives a single curve. Synthetic Daidzin added to the RP extract acquires the bioavailability of the endogenous Daidzin that exists naturally in the extract. These results show that (i) Daidzin is the major active principle in methanol extracts of RP, and (ii) additional constituents in the methanol extract of RP assist uptake of Daidzin in golden hamsters.

  • Daidzin and daidzein suppress free-choice ethanol intake by Syrian golden hamsters.
    Proceedings of the National Academy of Sciences of the United States of America, 1993
    Co-Authors: Wing-ming Keung, Bert L. Vallee
    Abstract:

    Abstract Syrian Golden hamsters prefer and consume large and remarkably constant amounts of ethanol in a simple two-bottle free-choice regimen. Ethanol intake is significantly suppressed by zimelidine, bromocriptine, buspirone, and lithium carbonate, pharmacological agents that have been shown to be beneficial in controlling ethanol intake in alcohol-dependent humans. These results suggest that this ethanol-drinking animal model has high "predictive validity" and can be used effectively in the search for and identification of new agents for the treatment of alcohol abuse. The model has enabled us to confirm the putative antidipsotropic effect of Radix puerariae (RP), an herb long used in traditional Chinese medicine for the treatment of patients who abuse alcohol. A crude extract of RP at a dose of 1.5 g.kg-1 x day-1 significantly suppresses (> 50%) the free-choice ethanol intake of Golden hamsters. Moreover, two major constituents of RP, daidzein (4',7-dihydroxyisoflavone) and Daidzin (the 7-glucoside of daidzein), were also shown to suppress free-choice ethanol intake. Daidzin and daidzein, at doses of 150 and 230 mg.kg-1 x day-1, respectively, suppress ethanol intake by > 50%. RP, daidzein, and Daidzin treatment do not significantly affect the body weight and water or food intake of the hamsters. These findings identify a class of compounds that offer promise as safe and effective therapeutic agents for alcohol abuse.

Zahra Emam-djomeh - One of the best experts on this subject based on the ideXlab platform.

  • Effect of ultrasound assisted extraction upon the Genistin and Daidzin contents of resultant soymilk
    Journal of Food Science and Technology, 2014
    Co-Authors: Ronak Fahmi, Faramarz Khodaiyan, Rezvan Pourahmad, Zahra Emam-djomeh
    Abstract:

    The purpose of this study was to determine the effect of ultrasound treatment on the contents of Daidzin, genistin, and their respective aglycones, daidzein and genistein, in resultant soymilk. Soybean slurry was exposed to ultrasound treatment, filtered, and placed in an ultrasound cleaning bath set with different frequencies (35and 130 KHz), treatment temperatures (20 and 40 °C), and times (20, 40, and 60 min). Concentrations for these isoflavones were determined using reverse-phase high-performance liquid chromatography. Results indicated that both frequencies significantly ( p  

Chan Uk Park - One of the best experts on this subject based on the ideXlab platform.

  • Formation of succinyl genistin and succinyl Daidzin by Bacillus species.
    Journal of food science, 2010
    Co-Authors: Chan Uk Park, Min Kyu Jeong, Min Hee Park, Joodong Yeu, Myeong Soo Park, Mi-ja Kim, Seon Min Ahn, Pahn-shick Chang, Jaehwan Lee
    Abstract:

    6''-O-Succinyl-4'-hydroxyisoflavone-7-O-beta-D-glucopyranoside (succinyl-beta-Daidzin) and 6''-O-succinyl-6,4'-dihydroxyisoflavone-7-O-beta-D-glucopyranoside (succinyl-beta-genistin), 2 new isoflavone metabolites, are found in cheonggukjang or natto, traditional soy-based foods fermented with Bacillus species. Standard isoflavones including Daidzin, genistin, daidzein, and genistein, and mixtures of isoflavones extracted from roasted soybeans were added to the medium growing Bacillus subtilis or B. subtilis natto and formation of succinyl-beta-Daidzin and succinyl-beta-genistin were monitored by high-performance liquid chromatography (HPLC). Samples containing Bacillus with Daidzin and genistin produced succinyl-beta-Daidzin and succinyl-beta-genistin, respectively, while those with daidzein and genistein did not produce succinyl derivatives. Daidzin in samples with B. subtilis and B. subtilis natto decreased by 39.7% and 10.7%, respectively, for 4 h incubation while genistin decreased by 66.8% and 17.6%, respectively. Genistein decreased faster than daidzein during incubation with B. subtilis or B. subtilis natto without formation of succinyl derivatives. In the case of mixture of isoflavones, succinyl derivatives increased and beta-glucosides and aglycones of isoflavones decreased significantly for 8 h incubation (P < 0.05).

Donghyun Kim - One of the best experts on this subject based on the ideXlab platform.

  • The effects of Daidzin and its aglycon, daidzein, on the scopolamineinduced memory impairment in male mice
    Archives of pharmacal research, 2010
    Co-Authors: Donghyun Kim, Hyun Ah Jung, Jin Park, Jong Min Kim, Seungjoo Lee, Jae Su Choi, Jae Hoon Cheong, Jong Hoon Ryu
    Abstract:

    In this study, the effect of Daidzin or daidzein isolated from Pueraria lobata on the memory impairments induced by scopolamine was assessed in male mice using the passive avoidance and the Morris water maze tasks. Administration of Daidzin (5 mg/kg) or daidzein (5 mg/kg) significantly reversed the scopolamine (1 mg/kg)-induced cognitive impairments in male mice as evidenced by the passive avoidance test (p < 0.05) and on the Morris water maze test (p < 0.05). Moreover, the ameliorating effects of Daidzin or daidzein were antagonized by tamoxifen (1 mg/kg), the nonspecific estrogen receptor antagonist. These results indicate that Daidzin or daidzein may be useful in cognitive impairment induced by cholinergic dysfunction, and this beneficial effect is mediated, in part, via estrogen receptor.

  • the role of intestinal microflora on daidzein excretion in urine in humans treated with chungpesagantang
    Archives of Pharmacal Research, 2007
    Co-Authors: Youngsuk Kim, Eunkyung Park, Hwajun Lee, Kiho Cho, Hyungsup Bae, Donghyun Kim
    Abstract:

    This study examined the relationship between the metabolism of the constituents of herbal medicines by human intestinal microflora and the level of metabolites excreted in the urine. This was performed by administering Chungpesagantang (CST) to volunteers and measuring their fecal metabolic activity CST to and urine excretion of daidzein, one of the metabolite of CST. The metabolic activity of of CST dadizein was 54.8 ± 16.7 mmol/h/g wet feces. When CST was administered orally to the subjects, the amount of daidzein excreted in the urine over 24 h was 103.7 ± 55.8 mg, which accounted for 20.2% of the puerarin, Daidzin and daidzein contained in CST. However, neither puerarin nor Daidzin were excreted in the urine. The profile of daidzein excreted in the urine was found to be in proportion to that of the metabolic activity of the CST components. This suggests that the daidzein level excreted in the urine of the subjects administered CST is associated with the daidzein glycoside-hydrolyzing activity of the fecal microflora.

  • Intestinal bacteria activate estrogenic effect of main constituents puerarin and Daidzin of Pueraria thunbergiana.
    Biological & pharmaceutical bulletin, 2006
    Co-Authors: Eunkyung Park, Ji-eun Shin, Eun-ah Bae, Young-chul Lee, Donghyun Kim
    Abstract:

    To understand the relationship between the metabolites and estrogenic activity of the main isoflavones puerarin and Daidzin of the rhizome of Pueraria thunbergiana (PT, family Leguminosae), PT and its isoflavones were transformed by human intestinal bacteria and their estrogenic effects were investigated. All human fecal specimens hydrolyzed puerarin and Daidzin to daidzein, but their hydrolyzing activities varied dependenting on the individuals. All intestinal bacteria isolated from human also hydrolyzed Daidzin to daidzein, but a few bacteria transformed puerarin to daidzein. When the estrogenic effect of PT, puerarin and Daidzin was compared with those of their metabolites, the metabolites more potently increased proliferation of MCF-7 cells than PT, puerarin and Daidzin. The metabolite daidzein also potently increased estrogen-response c-fos mRNA and PR protein expressions. These findings suggest that intestinal bacteria, which can hydrolyze puerarin and/or Daidzin, may activate a potent estrogenic activity of PT.

  • antithrombotic and antiallergic activities of daidzein a metabolite of puerarin and Daidzin produced by human intestinal microflora
    Biological & Pharmaceutical Bulletin, 2002
    Co-Authors: Minkyung Choo, Eunkyung Park, Haekyung Yoon, Donghyun Kim
    Abstract:

    To evaluate the antithrombotic activities of puerarin and Daidzin from the rhizome of Pueraria lobata, in vitro and ex vivo inhibitory activities of these compounds and their metabolite, daidzein, were measured. These compounds inhibited ADP- and collagen-induced platelet aggregation. Daidzein was the most potent. However, when puerarin and Daidzin were intraperitoneally administered, their antiaggregation activities were weaker than when these compounds were administered orally. When in vivo antithrombotic activities of these compounds against collagen and epinephrine were measured, these compounds showed significant protection from death due to pulmonary thrombosis in mice. To evaluate the antiallergic activity of puerarin, Daidzin, and daidzein, their inhibitory effects on the release of β-hexosaminidase from RBL 2H3 cells and on the passive cutaneous anaphylaxis (PCA) reaction in mice were examined. Daidzein exhibited potent inhibitory activity on the β-hexosaminidase release induced by DNP-BSA and potently inhibited the PCA reaction in rats. Daidzein administered intraperitoneally showed the strongest inhibitory activity and significantly inhibited the PCA reaction at doses of 25 and 50 mg/kg with inhibitory activity of 37 and 73%, respectively. The inhibitory activity of intraperitoneally administered daidzein was stronger than those of intraperitoneally and orally administered puerarin and Daidzin. Therefore we believe that puerarin and Daidzin in the rhizome of Pueraria lobata are prodrugs, which have antiallergic and antithrombotic activities, produced by intestinal microflora.

  • Metabolism of puerarin and Daidzin by human intestinal bacteria and their relation to in vitro cytotoxicity.
    Biological & pharmaceutical bulletin, 1998
    Co-Authors: Donghyun Kim, Eun-ah Bae, Myung Joo Han
    Abstract:

    When puerarin or Daidzin were incubated for 24 h with human intestinal bacteria, two metabolites, daidzein and calycosin, were produced from them, respectively. The metabolic time course of puerarin was as follows: at an early time, puerarin was converted to Daidzin, and then calycosin. The metabolic time course of Daidzin by human intestinal bacteria was also similar to that of puerarin. The in vitro cytotoxicities of these metabolites, calycosin and daidzein, were superior to those of puerarin and daidzein.