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Fengrui Song - One of the best experts on this subject based on the ideXlab platform.
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the metabolic fingerprint of the compatibility of radix aconite and radix paeoniae alba and its effect on cyp450 enzymes
Acta pharmaceutica Sinica, 2014Co-Authors: Yunfeng Bi, Zifeng Pi, Zhong Zheng, Fengrui SongAbstract:Abstract Using a UPLC-MS/MS (MRM) and cocktail probe substrates method, the metabolic fingerprint of the compatibility of Radix Aconite (RA) and Radix Paeoniae Alba (RPA) and its effect on CYP450 enzymes were investigated. These main CYP isoforms include CYP 1A2, CYP 2C, CYP 2E1, CYP 2D and CYP 3A. Compared with the inhibition effect of RA Decoctions on CYP450 isoforms, their co-Decoctions of RA and RPA with different proportions can decrease RA' inhibition on CYP3A, CYP2D, CYP2C and CYP1A2, but can not reduce RA' effect on CYP2E1. The metabolic fingerprints of RA Decoction and co-Decoctions with different proportions of RPA in CYP450 of rat liver were analyzed by UPLC-MS. Compared with the metabolic fingerprints of RA Decoction, the intensity of diester-diterpenoid aconitum alkaloids decreased significantly, while the intensity of monoester-diterpenoid alkaloids significantly increased in the metabolic fingerprints of co-Decoctions of RA and RPA. The results suggest that RA coadministration with RPA increased the degradation of toxic alkaloid and show the effect of toxicity reducing and efficacy enhancing.
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comparison of intracorporal absorption of hypaconitine in heishunpian Decoction and its compound recipe Decoction by ultra performance liquid chromatography quadrupole time of flight mass spectrometry
Chinese Journal of Chromatography, 2011Co-Authors: Zifeng Pi, Fengrui SongAbstract:: An ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) method was developed for the determination of hypaconitine in the plasma of the rats administered with Heishunpian (HSP) Decoction, Zhufu (ZF) Decoction and Gancaofuzi (GF) Decoction, separately. Hypaconitine and the internal standard were separated on a ZORBAX Extend-C18 column. The concentration-time curve of hypaconitine in the plasma was protracted, and pharmacokinetic parameters were calculated, then the pharmacokinetic comparison of hypaconitine between HSP, ZF and GF was carried out for the first time. The results of the methodological test showed that the plasma concentration of hypaconitine presented good linear relationship in the range of 0.02-10 ng/mL. The results of the sample determination showed that the absorptive degree of nfrom ZF Decoction in the plasma was lower than that from HSP Decoction, while the absorptive degree of hypaconitine from GF Decoction in plasma was higher than that from HSP Decoction. It was deduced that some components in ZF Decoction can restrain the absorption of hypaconitine in plasma, and some components in GF Decoction may promote the absorption of hypaconitine in plasma, and the difference in drug effects between ZF Decoction and GF Decoction may derive from the different absorptive degrees of hypaconitine in plasma. The pharmacokinetic property of hypaconitine was proposed to be non-linear dynamics on the basis of the drug elimination half life (T(1/2)) and the area under the plasma concentration-time curve (AUC).
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comparison of intracorporal absorption of hypaconitine in heishunpian Decoction and its compound recipe Decoction by ultra performance liquid chromatography quadrupole time of flight mass spectrometry
Chinese Journal of Chromatography, 2011Co-Authors: Zifeng Pi, Fengrui SongAbstract:: An ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) method was developed for the determination of hypaconitine in the plasma of the rats administered with Heishunpian (HSP) Decoction, Zhufu (ZF) Decoction and Gancaofuzi (GF) Decoction, separately. Hypaconitine and the internal standard were separated on a ZORBAX Extend-C18 column. The concentration-time curve of hypaconitine in the plasma was protracted, and pharmacokinetic parameters were calculated, then the pharmacokinetic comparison of hypaconitine between HSP, ZF and GF was carried out for the first time. The results of the methodological test showed that the plasma concentration of hypaconitine presented good linear relationship in the range of 0.02-10 ng/mL. The results of the sample determination showed that the absorptive degree of nfrom ZF Decoction in the plasma was lower than that from HSP Decoction, while the absorptive degree of hypaconitine from GF Decoction in plasma was higher than that from HSP Decoction. It was deduced that some components in ZF Decoction can restrain the absorption of hypaconitine in plasma, and some components in GF Decoction may promote the absorption of hypaconitine in plasma, and the difference in drug effects between ZF Decoction and GF Decoction may derive from the different absorptive degrees of hypaconitine in plasma. The pharmacokinetic property of hypaconitine was proposed to be non-linear dynamics on the basis of the drug elimination half life (T(1/2)) and the area under the plasma concentration-time curve (AUC).
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exploring the ester exchange reactions of diester diterpenoid alkaloids in the aconite Decoction process by electrospray ionization tandem mass spectrometry
Rapid Communications in Mass Spectrometry, 2003Co-Authors: Yong Wang, Fengrui Song, Zhiqiang Liu, Lei Shi, Shuying LiuAbstract:The chemical components in the Decoctions of Chinese herbal medicines are not always the same as those in the crude herbs because of the insolubility or instability of some compounds. In this work electrospray ionization tandem mass spectrometry was used to explore the ester-exchange reactions for aconitine-type diester-diterpenoid alkaloids occurring during the process of decocting aconite root. The aconitines were screened in a diverse range of samples, including crude aconite, Decoction of crude aconite, residues from Decoction of crude aconite, prepared aconite, Decoction of prepared aconite, Decoction of prepared aconite with added palmitic acid, and Decoction of a mixture of mesaconitine and hypaconitine standards with liquorice root. It was found that diester-diterpenoid aconitines were converted into lipo-alkaloids as well as monoester alkaloids by the Decoction of aconite.
Isabel C.f.r. Ferreira - One of the best experts on this subject based on the ideXlab platform.
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melissa officinalis l Decoctions as functional beverages a bioactive approach and chemical characterization
Food & Function, 2015Co-Authors: Marcio Carocho, Celestino Santosbuelga, Ana Ciric, Lillian Barros, Mirko Sokovic, Ricardo C. Calhelha, Patricia Morales, Isabel C.f.r. FerreiraAbstract:Lemon balm (Melissa officinalis L.) is a member of the Lamiaceae family with a long story of human consumption. It has been consumed for decades, directly in food and as a Decoction or an infusion for its medicinal purposes. In this manuscript, a detailed chemical characterization of the Decoction of this plant is described, encompassing antimicrobial, antioxidant and antitumor activities. Rosmarinic acid and lithospermic acid A were the most abundant phenolic compounds. Quinic acid, fructose, glucose and γ-tocopherol were the most abundant within their groups of molecules. M. officinalis Decoctions were active against a wide range of microorganisms, Pseudomonas aeruginosa and Salmonella typhimurium, and Penicillium funiculosum being the most sensitive bacteria and fungi, respectively. The growth inhibition of different human tumor cell lines (mainly MCF-7 and HepG2) was also observed, as also high free radical scavenging activity and reducing power. This manuscript highlights some beneficial effects of these functional beverages.
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nutritional parameters of infusions and Decoctions obtained from fragaria vesca l roots and vegetative parts
Lwt - Food Science and Technology, 2015Co-Authors: Lillian Barros, Patricia Morales, Maria Ines Dias, Maria De Cortes Sanchezmata, Beatriz M P P Oliveira, Isabel C.f.r. FerreiraAbstract:Abstract Fragaria vesca L. (wild strawberry) roots and vegetative parts are commonly used in infusions and Decoctions for different medicinal purposes. The composition in non-nutrients (mainly phenolic compounds) has previously been reported, but the contribution in nutritional compounds has not been researched. Therefore, chemical parameters with nutritional role, namely macronutrients, mineral components, some vitamins (ascorbic acid, folate and tocopherols), as well as, fatty acids, soluble sugars and organic acids, present in F. vesca roots and vegetative parts were evaluated using commercial and wild samples. Furthermore, their infusions and Decoctions were also fully characterized; as well as the percentages of vitamins and minerals released for the aqueous preparations. The processing steps, the collection region and also the physiological state in which the samples were collected could influence the differences found between commercial and wild samples. The infusion and Decoction preparations showed significantly high released percentages of folate and minerals, and also allowed the detection of xylose, proving to be more effective for soluble sugars extraction. Roots and vegetative parts of F. vesca , normally consumed as infusions and Decoctions, can be sources of macro and micronutrients.
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Development of a functional dairy food: Exploring bioactive and preservation effects of chamomile (Matricaria recutita L.)
Journal of Functional Foods, 2015Co-Authors: Cristina Caleja, Joao C M Barreira, Amilcar L. Antonio, M. Beatriz P.p. Oliveira, Ana Ciric, Lillian Barros, Celestino Santos-buelga, Mirko Sokovic, Isabel C.f.r. FerreiraAbstract:The antioxidant (free radical scavenging activity, reducing power and lipid peroxidation inhibition) and antimicrobial (against bacteria and fungi) potential of Matricaria recutita L. (chamomile) extracts obtained by Decoction was demonstrated. The characterization of the extracts highlighted dicaffeoyl-2,7-anhydro-3-deoxy-2-octulopyranosonic acid (diCDOA) and luteolin-. O-glucuronide as the main phenolic compounds. Extracts containing these natural ingredients were incorporated into cottage cheese, and the prepared products were compared with samples incorporated with chamomile powder and plain cottage cheese (control). Nutritional composition, colour and antioxidant activity of all samples were evaluated along storage time. Chamomile Decoctions (natural bioactive ingredient) did not alter significantly the nutritional and fatty acid profiles of cottage cheese, but improved its antioxidant potential, independent of the storage time. Moreover, it increased the shelf life since only the control samples showed signs of degradation after 14 days of storage. The development of this novel functional dairy product emphasizes the bioactive and preservation potential of chamomile.
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castanea sativa mill flowers amongst the most powerful antioxidant matrices a phytochemical approach in Decoctions and infusions
BioMed Research International, 2014Co-Authors: Marcio Carocho, Celestino Santosbuelga, Lillian Barros, Patricia Morales, Albino Bento, Isabel C.f.r. FerreiraAbstract:Infusions and Decoction of chestnut tree flowers have been used for different medical purposes, but their phytochemical profile and antioxidant activity are still mostly unknown. Herein, Decoctions and infusions of flowers from the two most appreciated chestnut cultivars (longal and judia) in Tras-os-Montes, Portugal, were prepared and characterized with regard to their content in free sugars, organic acids, and phenolic compounds, such as flavonoids and hydrolyzable tannins, and their antioxidant activity. Overall, the Decoction of the cultivar judia was the sample with both the highest quantity of flavonoids and antioxidant activity. The phenolic compound with the highest abundance in all samples was trigalloyl-HHDP-glucoside, followed by pentagalloyl glucoside. The sample with the highest quantity of total phenolic compounds was judia infusion, closely followed by longal Decoction, which also gave the highest quantities of ellagitannins. Regarding sugars and organic acids, the profiles were more similar. These results corroborate ancestral claims of the health benefits of infusions and Decoctions of chestnut flowers.
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Nutrients, phytochemicals and bioactivity of wild Roman chamomile: a comparison between the herb and its preparations.
Food chemistry, 2012Co-Authors: Rafaela Guimarães, Lillian Barros, Celestino Santos-buelga, Ricardo C. Calhelha, Ana Maria Carvalho, Maria João R. P. Queiroz, Montserrat Dueñas, Isabel C.f.r. FerreiraAbstract:Roman chamomile, Chamaemelum nobile L. (Asteraceae), has been used for medicinal applications, mainly through oral dosage forms (Decoctions and infusions). Herein, the nutritional characterisation of C. nobile was performed, and herbal material and its Decoction and infusion were submitted to an analysis of phytochemicals and bioactivity evaluation. The antioxidant activity was determined by free radicals scavenging activity, reducing power and inhibition of lipid peroxidation, the antitumour potential was tested in human tumour cell lines (breast, lung, colon, cervical and hepatocellular carcinomas), and the hepatotoxicity was evaluated using a porcine liver primary cell culture. C. nobile proved to be an equilibrated valuable herb rich in carbohydrates and proteins, and poor in fat, providing tocopherols, carotenoids and essential fatty acids (C18:2n6 and C18:3n3). Moreover, the herb and its infusion are a source of phenolic compounds (flavonoids such as flavonols and flavones, phenolic acids and derivatives) and organic acids (oxalic, quinic, malic, citric and fumaric acids) that showed antioxidant and antitumour activities, without hepatotoxicity. The most abundant compounds in the plant extract and infusion were 5-O-caffeoylquinic acid and an apigenin derivative. These, as well as other bioactive compounds, are affected in C. nobile Decoction, leading to a lower antioxidant potential and absence of antitumour potential. The plant bioactivity could be explored in the medicine, food, and cosmetic industries.
Susana M Cardoso - One of the best experts on this subject based on the ideXlab platform.
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salvia elegans salvia greggii and salvia officinalis Decoctions antioxidant activities and inhibition of carbohydrate and lipid metabolic enzymes
Molecules, 2018Co-Authors: Olivia R Pereira, Marcelo D Catarino, Andrea F Afonso, Artur M S Silva, Susana M CardosoAbstract:Salvia elegans Vahl., Salvia greggii A. Gray, and Salvia officinalis L. Decoctions were investigated for their health-benefit properties, in particular with respect to antioxidant activity and inhibitory ability towards key enzymes with impact in diabetes and obesity (α-glucosidase, α-amylase and pancreatic lipase). Additionally, the phenolic profiles of the three Decoctions were determined and correlated with the beneficial properties. The S. elegans Decoction was the most promising in regard to the antioxidant effects, namely in the scavenging capacity of the free radicals DPPH•, NO• and O2•–, and the ability to reduce Fe3+, as well as the most effective inhibitor of α-glucosidase (EC50 = 36.0 ± 2.7 μg/mL vs. EC50 = 345.3 ± 6.4 μg/mL and 71.2 ± 5.0 μg/mL for S. greggii and S. officinalis, respectively). This superior activity of the S. elegans Decoction over those of S. greggii and S. officinalis was, overall, highly correlated with its richness in caffeic acid and derivatives. In turn, the S. officinalis Decoction exhibited good inhibitory capacity against xanthine oxidase activity, a fact that could be associated with its high content of flavones, in particular the glycosidic forms of apigenin, scutellarein and luteolin.
Jing Cao - One of the best experts on this subject based on the ideXlab platform.
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comparative pharmacokinetic study of paeoniflorin after oral administration of Decoction of radix paeoniae rubra and radix paeoniae alba in rats
Journal of Ethnopharmacology, 2008Co-Authors: Changhong Wang, Zhengtao Wang, Xuemei Cheng, Rui Wang, Jing CaoAbstract:An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of Decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69+/-1.46 and 1.46+/-0.29 (p<0.05)microg/ml at 1.67+/-0.43 and 0.80+/-0.35 h (p<0.05), respectively. Compared to the AUC (18.85+/-7.54 microg h/ml) after oral administration of the paeoniflorin solution, a smaller AUC (10.61+/-1.51 microg h/ml, p<0.05) and a larger AUC (24.89+/-7.41 microg h/ml) of paeoniflorin after oral administration of the Decoctions of Radix Paeoniae Alba and Radix Paeoniae Rubra were obtained, respectively. There were statistically significant differences in pharmacokinetic parameters of paeoniflorin including the t(max), C(max), AUC, t(1/2), CL, and V(d) among the animals orally administered the Decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba. In particular, the parameters of t(max), C(max), and AUC of paeoniflorin were remarkably increased (P<0.05, P<0.001) when oral administering paeoniflorin in the Decoctions of Radix Paeoniae Rubra, but t(1/2), V(d), and CL were decreased (P<0.05 or P<0.01), in comparison of the Decoction of Radix Paeoniae Alba.
Akira Yagi - One of the best experts on this subject based on the ideXlab platform.
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simultaneous high performance liquid chromatographic determination of puerarin daidzin paeoniflorin liquiritin cinnamic acid cinnamaldehyde and glycyrrhizin in kampo medicines
Journal of Pharmaceutical and Biomedical Analysis, 1999Co-Authors: Nobuyuki Okamura, Hirotsugu Miki, Hiroshi Orii, Yasue Masaoka, Satie Yamashita, Hiroshi Kobayashi, Akira YagiAbstract:We report a high-performance liquid chromatographic method to determine the quantities of puerarin, daidzin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizin in Kampo medicine. All seven compounds were separated in less than 30 min with a Wakosil-II 5C18 AR column by linear gradient elution using 0.01% (v/v) phosphoric acid–acetonitrile (0 min 90:10, 10 min 88:12, 22 min 70:30, 30 min 30:70) as the mobile phase at a flow-rate of 1.0 ml min−1, and detection at 250 nm. The detection limits of these compounds are 0.15–0.3 μM with response linearity. This method was applied to determine the quantities in eight Kampo Decoctions; Mao-to, Makyo-yokukan-to, Makyo-kanseki-to, Yokuinin-to, Sho-seiryu-to, Keima-kakuhan-to, Kakkon-to and Kakkon-to-ka-senkyu-sin’i. Glycyrrhizin content was lower in both the Decoction and the methanol-diluted Decoction of Sho-seiryu-to compared with the others. Low pH due to organic acids of Schisandrae fructus in the Decoction caused inhibition for glycyrrhizin dissolution in Sho-seiryu-to.
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Simultaneous determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine in Kampo medicines by high-performance liquid chromatography.
Journal of Pharmaceutical and Biomedical Analysis, 1999Co-Authors: Nobuyuki Okamura, Hirotsugu Miki, Yasue Masaoka, Satie Yamashita, T Harada, Y Nakamoto, M Tsuguma, H Yoshitomi, Akira YagiAbstract:A simultaneous high-performance liquid chromatographic method for the determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine (ephedrine alkaloids) in Kampo medicines which contain Ephedrae Herba was established. The analysis can be accomplished within 25 min with a Wakosil-II 5C18 HG column by isocratic elution using a mixture of water, acetonitrile and sodium dodecyl sulfate (65:35:0.4) as the mobile phase at a flow-rate of 1.0 ml min(-1), and detection at 210 nm. The detection limits of ephedrine alkaloids are 0.37-1.06 microM per injection (5 microl). This method was applied to analyze the quantities in eight Kampo Decoctions; Mao-to, Makyo-yokukan-to, Makyo-kanseki-to, Yokuinin-to, Sho-seiryu-to, Keima-kakuhan-to, Kakkon-to and Kakkon-to-ka-senkyu-sin'i. The concentration (per Ephedrae Herba gram) of ephedrine alkaloids was higher in the Makyo-kanseki-to Decoction than in the others. Calcium sulfate from Gypsum Fibrosum raised ephedrine alkaloids dissolution in the Makyo-kanseki-to Decoction.
