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Thiago Saads Carvalho - One of the best experts on this subject based on the ideXlab platform.

  • Toothpaste factors related to Dentine tubule occlusion and Dentine protection against erosion and abrasion
    Clinical Oral Investigations, 2019
    Co-Authors: Samira Helena João-souza, Leticia Oba Sakae, Adrian Lussi, Ana Cecilia Corrêa Aranha, Anderson Hara, Tommy Baumann, Tais Scaramucci, Thiago Saads Carvalho
    Abstract:

    Objectives To investigate the effect of toothpastes on Dentine Surface loss and tubule occlusion, and the association of toothpaste-related factors to each of the outcomes. Materials and methods One hundred and sixty human Dentine specimens were randomly distributed into 10 groups, according to different toothpastes. The specimens were submitted to artificial saliva (60 min), citric acid (3 min), and brushing abrasion (25 s; totalizing 2 min in toothpaste slurries). This was repeated five times and two outcome variables were analyzed: Dentine Surface loss (dSL; μm) and tubule occlusion by measurement of the total area of open tubules (Area-OT; μm^2). Data were analyzed with Kruskal-Wallis and Mann-Whitney tests (α = 0.05); bivariate and multivariate regressions were used to model the association of the chemical (pH, concentration of F^−, Ca^2+, and PO_4^3− and presence of Sn^2+) and physical (% weight of solid particles, particle size, and wettability) factors of the toothpastes to both outcome variables. Results Toothpastes caused different degrees of dSL and did not differ in Area-OT. All chemical and physical factors, except the presence of Sn^2+, were associated with dSL ( p  

  • Toothpaste factors related to Dentine tubule occlusion and Dentine protection against erosion and abrasion.
    Clinical Oral Investigations, 2019
    Co-Authors: Samira Helena João-souza, Leticia Oba Sakae, Adrian Lussi, Ana Cecilia Corrêa Aranha, Tommy Baumann, Tais Scaramucci, Anderson T. Hara, Thiago Saads Carvalho
    Abstract:

    OBJECTIVES To investigate the effect of toothpastes on Dentine Surface loss and tubule occlusion, and the association of toothpaste-related factors to each of the outcomes. MATERIALS AND METHODS One hundred and sixty human Dentine specimens were randomly distributed into 10 groups, according to different toothpastes. The specimens were submitted to artificial saliva (60 min), citric acid (3 min), and brushing abrasion (25 s; totalizing 2 min in toothpaste slurries). This was repeated five times and two outcome variables were analyzed: Dentine Surface loss (dSL; μm) and tubule occlusion by measurement of the total area of open tubules (Area-OT; μm2). Data were analyzed with Kruskal-Wallis and Mann-Whitney tests (α = 0.05); bivariate and multivariate regressions were used to model the association of the chemical (pH, concentration of F-, Ca2+, and PO43- and presence of Sn2+) and physical (% weight of solid particles, particle size, and wettability) factors of the toothpastes to both outcome variables. RESULTS Toothpastes caused different degrees of dSL and did not differ in Area-OT. All chemical and physical factors, except the presence of Sn2+, were associated with dSL (p 

Junji Tagami - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Silver Diamine Fluoride on Reducing Candida albicans Adhesion on Dentine
    Mycopathologia, 2020
    Co-Authors: Rima Zakzuk Alshahni, Mohamed Mahdi Alshahni, Noriko Hiraishi, Koichi Makimura, Junji Tagami
    Abstract:

    Background Candida albicans is the most frequent pathogenic fungus in oral cavities. It adheres to dental tissues as part of dental plaques and contributes to caries formation. Objectives To evaluate the effect of silver diamine fluoride (SDF) on reducing C. albicans adhesion on Dentine Surfaces. Methods Flat Dentine Surfaces were prepared from bovine dental disks, and samples were divided into three groups. The first and second groups were pretreated for 3 min with 299 mM or 2.99 M SDF, respectively, and the third group (control) did not undergo any SDF pretreatment. All samples were washed, inoculated with C. albicans suspension onto their Dentine Surface, incubated at 30 °C for 6 h, and washed again to remove any nonadherent cells. The abundance of adherent cells was investigated using colorimetric and real-time polymerase chain reaction approaches. Subsequently, the morphological changes in C. albicans by pretreatment with SDF were observed under a scanning electron microscope (SEM). Results SDF inhibited candidal growth at concentrations as low as 2.99 µM. Dentine disks pretreated with 299 mM or 2.99 M SDF displayed significantly fewer adhered cells as compared with the control group. Upon pretreatment with SDF, SEM images showed severe morphological changes in the cellular walls, in a dose-dependent manner, suggesting a fungicidal effect of SDF against the yeast. Conclusion SDF should be considered for clinical applications aimed at inhibiting dental plaque caused by C. albicans , particularly in children and elderly individuals.

  • Effect of Silver Diamine Fluoride on Reducing Candida albicans Adhesion on Dentine.
    Mycopathologia, 2020
    Co-Authors: Rima Zakzuk Alshahni, Mohamed Mahdi Alshahni, Noriko Hiraishi, Koichi Makimura, Junji Tagami
    Abstract:

    Candida albicans is the most frequent pathogenic fungus in oral cavities. It adheres to dental tissues as part of dental plaques and contributes to caries formation. To evaluate the effect of silver diamine fluoride (SDF) on reducing C. albicans adhesion on Dentine Surfaces. Flat Dentine Surfaces were prepared from bovine dental disks, and samples were divided into three groups. The first and second groups were pretreated for 3 min with 299 mM or 2.99 M SDF, respectively, and the third group (control) did not undergo any SDF pretreatment. All samples were washed, inoculated with C. albicans suspension onto their Dentine Surface, incubated at 30 °C for 6 h, and washed again to remove any nonadherent cells. The abundance of adherent cells was investigated using colorimetric and real-time polymerase chain reaction approaches. Subsequently, the morphological changes in C. albicans by pretreatment with SDF were observed under a scanning electron microscope (SEM). SDF inhibited candidal growth at concentrations as low as 2.99 µM. Dentine disks pretreated with 299 mM or 2.99 M SDF displayed significantly fewer adhered cells as compared with the control group. Upon pretreatment with SDF, SEM images showed severe morphological changes in the cellular walls, in a dose-dependent manner, suggesting a fungicidal effect of SDF against the yeast. SDF should be considered for clinical applications aimed at inhibiting dental plaque caused by C. albicans, particularly in children and elderly individuals.

  • effect of smear layer deproteinizing on resin Dentine interface with self etch adhesive
    Journal of Dentistry, 2014
    Co-Authors: Ornnicha Thanatvarakorn, Masatoshi Nakajima, Taweesak Prasansuttiporn, Shizuko Ichinose, Richard M Foxton, Junji Tagami
    Abstract:

    Abstract Objectives This study aimed to investigate deproteinizing effect of sodium-hypochlorite (NaOCl) and mild acidic hypochlorous-acid (HOCl) pretreatment on smear layer-covered Dentine and to evaluate their effects on morphological characteristics of resin–Dentine interface with self-etch adhesive. Methods Human coronal-Dentine discs with standardized smear layer were pretreated with 6% NaOCl or 50 ppm HOCl for 15 s or 30 s. Their deproteinizing effects at the treated smear layer-covered Dentine Surfaces were determined by the measurement of amide:phosphate ratio using ATR-FTIR analysis. In addition, using TEM, micromorphological alterations of hybridized complex and nanoleakage expression were evaluated at the interface of a self-etch adhesive (Clearfil SE Bond) to the pretreated Dentine Surface with or without subsequent application of a reducing agent ( p -Toluenesulfinic acid salt; Accel ® ). Results Both pretreatments of NaOCl and HOCl significantly reduced the amide:phosphate ratio as compared with the no-pretreated group ( p Conclusions NaOCl and HOCl solutions could remove the organic component on the smear layer-covered Dentine, which could eliminate the hybridized smear layer created by self-etch adhesive, leading to the reduction of nanoleakage expression within hybrid layer. Clinical significance Smear layer deproteinizing could modify Dentine Surface, giving an appropriate substrate for bonding to self-etch adhesive system.

  • Effect of smear layer deproteinizing on resin–Dentine interface with self-etch adhesive
    Journal of Dentistry, 2013
    Co-Authors: Ornnicha Thanatvarakorn, Masatoshi Nakajima, Taweesak Prasansuttiporn, Shizuko Ichinose, Richard M Foxton, Junji Tagami
    Abstract:

    Abstract Objectives This study aimed to investigate deproteinizing effect of sodium-hypochlorite (NaOCl) and mild acidic hypochlorous-acid (HOCl) pretreatment on smear layer-covered Dentine and to evaluate their effects on morphological characteristics of resin–Dentine interface with self-etch adhesive. Methods Human coronal-Dentine discs with standardized smear layer were pretreated with 6% NaOCl or 50 ppm HOCl for 15 s or 30 s. Their deproteinizing effects at the treated smear layer-covered Dentine Surfaces were determined by the measurement of amide:phosphate ratio using ATR-FTIR analysis. In addition, using TEM, micromorphological alterations of hybridized complex and nanoleakage expression were evaluated at the interface of a self-etch adhesive (Clearfil SE Bond) to the pretreated Dentine Surface with or without subsequent application of a reducing agent ( p -Toluenesulfinic acid salt; Accel ® ). Results Both pretreatments of NaOCl and HOCl significantly reduced the amide:phosphate ratio as compared with the no-pretreated group ( p Conclusions NaOCl and HOCl solutions could remove the organic component on the smear layer-covered Dentine, which could eliminate the hybridized smear layer created by self-etch adhesive, leading to the reduction of nanoleakage expression within hybrid layer. Clinical significance Smear layer deproteinizing could modify Dentine Surface, giving an appropriate substrate for bonding to self-etch adhesive system.

Mario De Rosa - One of the best experts on this subject based on the ideXlab platform.

  • er yag laser treatments on Dentine Surface micro raman spectroscopy and sem analysis
    Journal of Dentistry, 2004
    Co-Authors: C Camerlingo, M Lepore, G M Gaeta, R Riccio, Carlo Riccio, A De Rosa, Mario De Rosa
    Abstract:

    Abstract Objective . This study aims to give an insight into the chemical and structural modifications occurring in laser treated Dentine Surface, as a tool to plan a new class of dental materials specific for the dental restoration when laser technologies are used. For this purpose the effect of Er:YAG (Erbium–Yttrium Aluminium Garnet) laser treatments on human Dentine Surface has been investigated. Methods . The Dentine Surface of periodontally compromised teeth cut along the main axis was treated in vitro with laser pulses having the same energy but time duration varying in the range of 100–1000 μs. The chemical characteristics and the morphology of the laser treated Surface have been studied with micro-Raman spectroscopy (μ-RS) and scansion electron microscopy (SEM). Results . The μ-RS and SEM analysis of laser treated teeth have been compared with those of Surfaces treated with conventional mechanical methodologies. The laser pulse time duration affects the Surface morphology and the chemistry of Dentine, mainly of organic components. The effects have been monitored by analysing the C–H group stretch vibration mode of the μ-RS spectrum. Conclusion . The laser treatment can determine different chemical microenvironments on the treated Dentine Surface, depending on the time duration of laser pulse used. Treatments with very long pulse results in a the Dentine Surface very similar to that obtained with a conventional mechanical drill, while with very short laser pulses a strong modification of the Dentine organic components has been observed.

  • er yag laser treatments on Dentine Surface micro raman spectroscopy and sem analysis
    Biosilico, 2004
    Co-Authors: M Lepore, C Camerlingo, G M Gaeta, R Riccio, Carlo Riccio, Mario De Rosa
    Abstract:

    This study investigates the chemical and structural modifications in laser treated Dentine Surface. For this purpose the effect of Er-YAG laser on human Dentine Surface has been investigated with micro-Raman spectroscopy and SEM microscopy.

Mônica Campos Serra - One of the best experts on this subject based on the ideXlab platform.

  • Study on the potential inhibition of root Dentine wear adjacent to fluoride-containing restorations
    Journal of Materials Science: Materials in Medicine, 2008
    Co-Authors: Cecilia Pedroso Turssi, Anderson Takeo Hara, Silvia Jorge Domiciano, Mônica Campos Serra
    Abstract:

    The purpose of this in vitro study was to determine whether the vicinity of root Dentine that had been restored with fluoride-releasing materials was at reduced risk for erosive/abrasive wear compared to root Dentine restored with a non-fluoride-containing material. According to a randomized complete block design, standardized cavities prepared on the Surface of 150 bovine root Dentine slabs were restored with glass-ionomer cement, resin-modified glass ionomer, polyacid-modified resin composite, fluoride-containing or conventional composite. Specimens were coated with two layers of an acid-resistant nail varnish exposing half of the Dentine Surface and half of the restoration. Subsequently, specimens were either eroded in an acidic drink or left uneroded, then exposed to artificial saliva and abraded in a toothbrushing machine. Wear depth in the vicinity of restorations was quantified by a stylus profilometer, based on the nonabraded areas surrounding the erosion/abrasion region. Two-way ANOVA did not demonstrate significant interaction between restoratives and eroded-uneroded Dentine ( p  = 0.5549) nor significant difference among restorative materials ( p  = 0.8639). Tukey’s test ascertained that the wear depth was higher for eroded than for uneroded groups. Fluoride-releasing materials seemed to negligibly inhibit wear in the vicinity of restored root Dentine subjected to erosive/abrasive challenges.

  • Abrasion of eroded root Dentine brushed with different toothpastes
    Clinical Oral Investigations, 2004
    Co-Authors: Márcio Menezes, Cecilia Pedroso Turssi, Anderson Takeo Hara, Danielle Cristine Furtado Messias, Mônica Campos Serra
    Abstract:

    This study evaluated the Surface roughness change and wear provided by different dentifrices on root Dentine previously exposed to erosive challenges. According to a randomized complete block design, 150 slabs of bovine root Dentine (6×3×2 mm) were ground flat and polished. In an area of 4×3 mm on the Dentine Surface, specimens were submitted to five erosive/abrasive events, each one composed by: exposure to Sprite Diet or distilled water for 5 min, then to a remineralizing solution for 1 min, and simulation of 5,000 brushing strokes. Four dentifrices—regular (RE), baking soda (BS), whitening (WT) and tartar control (TC)—and distilled water (CO), used as control, were compared. Final texture and the wear depth were evaluated using a profilometer. ANOVA did not show significant interaction, indicating that the effect of dentifrices on both Surface roughness change and wear did not depend on whether or not the Dentine was eroded ( p >0.05). There was no difference between abrasion of eroded and sound Dentine. The Tukey’s test revealed that WT, BS and TC provided the highest increase in Surface roughness differing from RE and CO. TC yielded the deepest wear of root Dentine, whereas RE and CO, the shallowest. No significant difference in wear among BS, TC and WT were observed. Within the limitations of this study, the data showed that abrasion of both eroded and sound root Dentine was dependent on the dentifrice used.

S. Nammour - One of the best experts on this subject based on the ideXlab platform.

  • Effect of acid etching duration on tensile bond strength of composite resin bonded to erbium:yttrium–aluminium–garnet laser-prepared Dentine. Preliminary study
    Lasers in Medical Science, 2010
    Co-Authors: M. Chousterman, D. Heysselaer, S. M. Dridi, F. Bayet, B. Misset, L. Lamard, A. Peremans, C. Nyssen-behets, S. Nammour
    Abstract:

    The purpose of this study was to compare the tensile bond strength of composite resin bonded to erbium:yttrium–aluminium–garnet (Er:YAG) laser-prepared Dentine after different durations of acid etching. The occlusal third of 68 human third molars was removed in order to expose the Dentine Surface. The teeth were randomly divided into five groups: group B (control group), prepared with bur and total etch system with 15 s acid etching [37% orthophosphoric acid (H_3PO_4)]; group L15, laser photo-ablated Dentine (200 mJ) (laser irradiation conditions: pulse duration 100 µs, air–water spray, fluence 31.45 J/ cm^2, 10 Hz, non-contact hand pieces, beam spot size 0.9 mm, irradiation speed 3 mm/s, and total irradiation time 2 x 40 s); group L30, laser prepared, laser conditioned and 30 s acid etching; group L60, laser prepared, laser conditioned and 60 s acid etching; group L90, laser prepared, laser conditioned and 90 s acid etching. A plot of composite resin was bonded onto each exposed Dentine and then tested for tensile bond strength. The values obtained were statistically analysed by analysis of variance (ANOVA) coupled with the Tukey–Kramer test at the 95 % level. A 90 s acid etching before bonding showed the best bonding value ( P