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Scott A Venners - One of the best experts on this subject based on the ideXlab platform.
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Occupational Exposure to Antineoplastic Drugs: Identification of Job Categories Potentially Exposed throughout the Hospital Medication System
Safety and health at work, 2020Co-Authors: Kay Teschke, Prescillia Ps Chua, Scott A Venners, Lynne NakashimaAbstract:Objectives: Studies examining healthcare workers’ exposure to antineoplastic Drugs have focused on the Drug preparation or Drug administration areas. However, such an approach has probably underestimated the overall exposure risk as the Drugs need to be delivered to the facility, transported internally and then disposed. The objective of this study is to determine whether Drug Contamination occurs throughout a facility and, simultaneously, to identify those job categories that are potentially exposed. Methods: This was a multi-site study based in Vancouver, British Columbia. Interviews were conducted to determine the departments where the Drugs travel. Subsequent site observations were performed to ascertain those surfaces which frequently came into contact with antineoplastic Drugs and to determine the job categories which are likely to contact these surfaces. Wipe samples were collected to quantify surface Contamination. Results: Surface Contamination was found in all six stages of the hospital medication system. Job categories consistently found to be at risk of exposure were nurses, pharmacists, pharmacy technicians, and pharmacy receivers. Up to 11 job categories per site may be at risk of exposure at some point during the hospital medication system. Conclusion: We found Drug Contamination on select surfaces at every stage of the medication system, which indicates the existence of an exposure potential throughout the facility. Our results suggest that a broader range of workers are potentially exposed than has been previously examined. These results will allow us to develop a more inclusive exposure assessment encompassing all healthcare workers that are at risk throughout the hospital medication system.
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Antineoplastic Drug Contamination in the urine of Canadian healthcare workers
International Archives of Occupational and Environmental Health, 2015Co-Authors: Kay Teschke, Paul A Demers, Hui Shen, Scott A VennersAbstract:Purpose The purpose of this study was to quantify the urine concentration of non-metabolized cyclophosphamide (CP), a commonly administered antineoplastic Drug, among potentially exposed Canadian healthcare workers and to identify factors associated with the Drug concentration levels. Methods Participants were asked to provide two sets of 24-h urine samples (at two different sampling events), and the level of CP was quantified using high-performance liquid chromatography–tandem mass spectrometry. In addition to demographic information, participants were surveyed regarding their frequency of handling of antineoplastic Drugs, safe Drug handling training, and known contact with CP on their work shift. Descriptive and inferential statistical analyses were performed. A backward stepwise linear mixed effect model was conducted to identify the factors associated with urine concentration levels. Results We collected 201 urine samples, and 55 % ( n = 111) had levels greater than the LOD of 0.05 ng/mL. The mean urinary CP concentration was 0.156 ng/mL, the geometric mean was 0.067 ng/mL, the geometric standard deviation was 3.18, the 75th percentile was 0.129 ng/mL, and the range was
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antineoplastic Drug Contamination in the urine of canadian healthcare workers
International Archives of Occupational and Environmental Health, 2015Co-Authors: Kay Teschke, Paul A Demers, Hui Shen, Scott A VennersAbstract:Purpose The purpose of this study was to quantify the urine concentration of non-metabolized cyclophosphamide (CP), a commonly administered antineoplastic Drug, among potentially exposed Canadian healthcare workers and to identify factors associated with the Drug concentration levels.
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antineoplastic Drug Contamination on the hands of employees working throughout the hospital medication system
Annals of Occupational Hygiene, 2014Co-Authors: Kay Teschke, Paul A Demers, Scott A VennersAbstract:Abstr A ct We previously reported that antineoplastic Drug Contamination is found on various work surfaces situated throughout the hospital medication system (process flow of Drug within a facility from initial delivery to waste disposal). The presence of Drug residual on surfaces suggests that healthcare workers involved in some capacity with the system may be exposed through dermal contact. The purpose of this paper was to determine the dermal Contamination levels of healthcare employees working throughout a hospital and to identify factors that may influence dermal Contamination. We selected participants from six hospitals and wiped the front and back of workers’ hands. Wipe samples were analyzed for cyclophosphamide (CP), a commonly used antineoplastic Drug, using high-performance liquid chromatography-tandem mass spectrometry. Participants were asked about their frequency of handling antineoplastic Drugs, known contact with CP on their work shift, gender, job title, and safe Drug handling training. In addition, participants were surveyed regarding their glove usage and hand washing practices prior to wipe sample collection. We collected a total of 225 wipe samples. Only 20% (N = 44) were above the limit of detection (LOD) of 0.36 ng per wipe. The average concentration was 0.36 ng per wipe, the geometric mean < LOD, the geometric standard deviation 1.98, and the range < LOD to 22.8 ng per wipe. Hospital employees were classified into eight different job categories and all categories had some dermal Contamination levels in excess of the LOD. The job category with the highest proportion of samples greater than the LOD were those workers in the Drug administration unit who were not responsible for Drug administration (volunteer, oncologist, ward aide, dietician). Of note, the highest recorded concentration was from a worker who had no known contact with CP on their work shift. Our results suggest that a broader range of healthcare workers than previously believed, including those that do not directly handle or administer the Drugs (e.g. unit clerks, ward aides, dieticians, and shipper/receivers), are at risk of exposure to antineoplastic Drugs. A review of control measures to minimize antineoplastic Drug exposure that encompasses a wide array of healthcare workers involved with the hospital medication system is recommended.
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antineoplastic Drug Contamination of surfaces throughout the hospital medication system in canadian hospitals
Journal of Occupational and Environmental Hygiene, 2013Co-Authors: Kay Teschke, Paul A Demers, Scott A VennersAbstract:We previously reported that there is a potential for antineoplastic Drug Contamination throughout the hospital medication system (process flow of Drug within a facility from delivery to waste disposal) due to the various surfaces contacted by health care workers. This article describes the Contamination of these frequently contacted surfaces as well as identifies factors that may be associated with surface Contamination. Surfaces which health care workers frequently contact were wiped and the concentration of cyclophosphamide (CP) was determined using high-performance liquid chromatography-tandem mass spectrometry. Descriptive and inferential statistical analyses were performed. A backward stepwise multiple linear regression was conducted to identify determinants associated with surface Contamination. Overall, 229 surfaces were sampled, most on two occasions, for a total of 438 surface wipes. The mean CP concentration was 0.201 ng/cm2, the geometric mean 0.019 ng/cm2, and the geometric standard deviation ...
Paul A Demers - One of the best experts on this subject based on the ideXlab platform.
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Antineoplastic Drug Contamination in the urine of Canadian healthcare workers
International Archives of Occupational and Environmental Health, 2015Co-Authors: Kay Teschke, Paul A Demers, Hui Shen, Scott A VennersAbstract:Purpose The purpose of this study was to quantify the urine concentration of non-metabolized cyclophosphamide (CP), a commonly administered antineoplastic Drug, among potentially exposed Canadian healthcare workers and to identify factors associated with the Drug concentration levels. Methods Participants were asked to provide two sets of 24-h urine samples (at two different sampling events), and the level of CP was quantified using high-performance liquid chromatography–tandem mass spectrometry. In addition to demographic information, participants were surveyed regarding their frequency of handling of antineoplastic Drugs, safe Drug handling training, and known contact with CP on their work shift. Descriptive and inferential statistical analyses were performed. A backward stepwise linear mixed effect model was conducted to identify the factors associated with urine concentration levels. Results We collected 201 urine samples, and 55 % ( n = 111) had levels greater than the LOD of 0.05 ng/mL. The mean urinary CP concentration was 0.156 ng/mL, the geometric mean was 0.067 ng/mL, the geometric standard deviation was 3.18, the 75th percentile was 0.129 ng/mL, and the range was
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antineoplastic Drug Contamination in the urine of canadian healthcare workers
International Archives of Occupational and Environmental Health, 2015Co-Authors: Kay Teschke, Paul A Demers, Hui Shen, Scott A VennersAbstract:Purpose The purpose of this study was to quantify the urine concentration of non-metabolized cyclophosphamide (CP), a commonly administered antineoplastic Drug, among potentially exposed Canadian healthcare workers and to identify factors associated with the Drug concentration levels.
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antineoplastic Drug Contamination on the hands of employees working throughout the hospital medication system
Annals of Occupational Hygiene, 2014Co-Authors: Kay Teschke, Paul A Demers, Scott A VennersAbstract:Abstr A ct We previously reported that antineoplastic Drug Contamination is found on various work surfaces situated throughout the hospital medication system (process flow of Drug within a facility from initial delivery to waste disposal). The presence of Drug residual on surfaces suggests that healthcare workers involved in some capacity with the system may be exposed through dermal contact. The purpose of this paper was to determine the dermal Contamination levels of healthcare employees working throughout a hospital and to identify factors that may influence dermal Contamination. We selected participants from six hospitals and wiped the front and back of workers’ hands. Wipe samples were analyzed for cyclophosphamide (CP), a commonly used antineoplastic Drug, using high-performance liquid chromatography-tandem mass spectrometry. Participants were asked about their frequency of handling antineoplastic Drugs, known contact with CP on their work shift, gender, job title, and safe Drug handling training. In addition, participants were surveyed regarding their glove usage and hand washing practices prior to wipe sample collection. We collected a total of 225 wipe samples. Only 20% (N = 44) were above the limit of detection (LOD) of 0.36 ng per wipe. The average concentration was 0.36 ng per wipe, the geometric mean < LOD, the geometric standard deviation 1.98, and the range < LOD to 22.8 ng per wipe. Hospital employees were classified into eight different job categories and all categories had some dermal Contamination levels in excess of the LOD. The job category with the highest proportion of samples greater than the LOD were those workers in the Drug administration unit who were not responsible for Drug administration (volunteer, oncologist, ward aide, dietician). Of note, the highest recorded concentration was from a worker who had no known contact with CP on their work shift. Our results suggest that a broader range of healthcare workers than previously believed, including those that do not directly handle or administer the Drugs (e.g. unit clerks, ward aides, dieticians, and shipper/receivers), are at risk of exposure to antineoplastic Drugs. A review of control measures to minimize antineoplastic Drug exposure that encompasses a wide array of healthcare workers involved with the hospital medication system is recommended.
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antineoplastic Drug Contamination of surfaces throughout the hospital medication system in canadian hospitals
Journal of Occupational and Environmental Hygiene, 2013Co-Authors: Kay Teschke, Paul A Demers, Scott A VennersAbstract:We previously reported that there is a potential for antineoplastic Drug Contamination throughout the hospital medication system (process flow of Drug within a facility from delivery to waste disposal) due to the various surfaces contacted by health care workers. This article describes the Contamination of these frequently contacted surfaces as well as identifies factors that may be associated with surface Contamination. Surfaces which health care workers frequently contact were wiped and the concentration of cyclophosphamide (CP) was determined using high-performance liquid chromatography-tandem mass spectrometry. Descriptive and inferential statistical analyses were performed. A backward stepwise multiple linear regression was conducted to identify determinants associated with surface Contamination. Overall, 229 surfaces were sampled, most on two occasions, for a total of 438 surface wipes. The mean CP concentration was 0.201 ng/cm2, the geometric mean 0.019 ng/cm2, and the geometric standard deviation ...
Kay Teschke - One of the best experts on this subject based on the ideXlab platform.
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Occupational Exposure to Antineoplastic Drugs: Identification of Job Categories Potentially Exposed throughout the Hospital Medication System
Safety and health at work, 2020Co-Authors: Kay Teschke, Prescillia Ps Chua, Scott A Venners, Lynne NakashimaAbstract:Objectives: Studies examining healthcare workers’ exposure to antineoplastic Drugs have focused on the Drug preparation or Drug administration areas. However, such an approach has probably underestimated the overall exposure risk as the Drugs need to be delivered to the facility, transported internally and then disposed. The objective of this study is to determine whether Drug Contamination occurs throughout a facility and, simultaneously, to identify those job categories that are potentially exposed. Methods: This was a multi-site study based in Vancouver, British Columbia. Interviews were conducted to determine the departments where the Drugs travel. Subsequent site observations were performed to ascertain those surfaces which frequently came into contact with antineoplastic Drugs and to determine the job categories which are likely to contact these surfaces. Wipe samples were collected to quantify surface Contamination. Results: Surface Contamination was found in all six stages of the hospital medication system. Job categories consistently found to be at risk of exposure were nurses, pharmacists, pharmacy technicians, and pharmacy receivers. Up to 11 job categories per site may be at risk of exposure at some point during the hospital medication system. Conclusion: We found Drug Contamination on select surfaces at every stage of the medication system, which indicates the existence of an exposure potential throughout the facility. Our results suggest that a broader range of workers are potentially exposed than has been previously examined. These results will allow us to develop a more inclusive exposure assessment encompassing all healthcare workers that are at risk throughout the hospital medication system.
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Antineoplastic Drug Contamination in the urine of Canadian healthcare workers
International Archives of Occupational and Environmental Health, 2015Co-Authors: Kay Teschke, Paul A Demers, Hui Shen, Scott A VennersAbstract:Purpose The purpose of this study was to quantify the urine concentration of non-metabolized cyclophosphamide (CP), a commonly administered antineoplastic Drug, among potentially exposed Canadian healthcare workers and to identify factors associated with the Drug concentration levels. Methods Participants were asked to provide two sets of 24-h urine samples (at two different sampling events), and the level of CP was quantified using high-performance liquid chromatography–tandem mass spectrometry. In addition to demographic information, participants were surveyed regarding their frequency of handling of antineoplastic Drugs, safe Drug handling training, and known contact with CP on their work shift. Descriptive and inferential statistical analyses were performed. A backward stepwise linear mixed effect model was conducted to identify the factors associated with urine concentration levels. Results We collected 201 urine samples, and 55 % ( n = 111) had levels greater than the LOD of 0.05 ng/mL. The mean urinary CP concentration was 0.156 ng/mL, the geometric mean was 0.067 ng/mL, the geometric standard deviation was 3.18, the 75th percentile was 0.129 ng/mL, and the range was
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antineoplastic Drug Contamination in the urine of canadian healthcare workers
International Archives of Occupational and Environmental Health, 2015Co-Authors: Kay Teschke, Paul A Demers, Hui Shen, Scott A VennersAbstract:Purpose The purpose of this study was to quantify the urine concentration of non-metabolized cyclophosphamide (CP), a commonly administered antineoplastic Drug, among potentially exposed Canadian healthcare workers and to identify factors associated with the Drug concentration levels.
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antineoplastic Drug Contamination on the hands of employees working throughout the hospital medication system
Annals of Occupational Hygiene, 2014Co-Authors: Kay Teschke, Paul A Demers, Scott A VennersAbstract:Abstr A ct We previously reported that antineoplastic Drug Contamination is found on various work surfaces situated throughout the hospital medication system (process flow of Drug within a facility from initial delivery to waste disposal). The presence of Drug residual on surfaces suggests that healthcare workers involved in some capacity with the system may be exposed through dermal contact. The purpose of this paper was to determine the dermal Contamination levels of healthcare employees working throughout a hospital and to identify factors that may influence dermal Contamination. We selected participants from six hospitals and wiped the front and back of workers’ hands. Wipe samples were analyzed for cyclophosphamide (CP), a commonly used antineoplastic Drug, using high-performance liquid chromatography-tandem mass spectrometry. Participants were asked about their frequency of handling antineoplastic Drugs, known contact with CP on their work shift, gender, job title, and safe Drug handling training. In addition, participants were surveyed regarding their glove usage and hand washing practices prior to wipe sample collection. We collected a total of 225 wipe samples. Only 20% (N = 44) were above the limit of detection (LOD) of 0.36 ng per wipe. The average concentration was 0.36 ng per wipe, the geometric mean < LOD, the geometric standard deviation 1.98, and the range < LOD to 22.8 ng per wipe. Hospital employees were classified into eight different job categories and all categories had some dermal Contamination levels in excess of the LOD. The job category with the highest proportion of samples greater than the LOD were those workers in the Drug administration unit who were not responsible for Drug administration (volunteer, oncologist, ward aide, dietician). Of note, the highest recorded concentration was from a worker who had no known contact with CP on their work shift. Our results suggest that a broader range of healthcare workers than previously believed, including those that do not directly handle or administer the Drugs (e.g. unit clerks, ward aides, dieticians, and shipper/receivers), are at risk of exposure to antineoplastic Drugs. A review of control measures to minimize antineoplastic Drug exposure that encompasses a wide array of healthcare workers involved with the hospital medication system is recommended.
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antineoplastic Drug Contamination of surfaces throughout the hospital medication system in canadian hospitals
Journal of Occupational and Environmental Hygiene, 2013Co-Authors: Kay Teschke, Paul A Demers, Scott A VennersAbstract:We previously reported that there is a potential for antineoplastic Drug Contamination throughout the hospital medication system (process flow of Drug within a facility from delivery to waste disposal) due to the various surfaces contacted by health care workers. This article describes the Contamination of these frequently contacted surfaces as well as identifies factors that may be associated with surface Contamination. Surfaces which health care workers frequently contact were wiped and the concentration of cyclophosphamide (CP) was determined using high-performance liquid chromatography-tandem mass spectrometry. Descriptive and inferential statistical analyses were performed. A backward stepwise multiple linear regression was conducted to identify determinants associated with surface Contamination. Overall, 229 surfaces were sampled, most on two occasions, for a total of 438 surface wipes. The mean CP concentration was 0.201 ng/cm2, the geometric mean 0.019 ng/cm2, and the geometric standard deviation ...
Jeffrey S. Barrett - One of the best experts on this subject based on the ideXlab platform.
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Modeling and simulation approaches to evaluate pharmacokinetic sampling Contamination from central venous catheters in pediatric pharmacokinetic studies of actinomycin-D: a report from the children’s oncology group
Cancer Chemotherapy and Pharmacology, 2012Co-Authors: Alena Y. Z. Edwards, Jeffrey M. Skolnik, Erin Dombrowsky, Dimple Patel, Jeffrey S. BarrettAbstract:Background The binding of Drugs to catheters can be a source variation in dosing chemotherapeutics. Drug Contamination from the dosing central venous line (CVL) can impact the reporting of pharmacokinetic (PK) results and analysis. Peripheral venipuncture avoids binding complications from the CVL but dissuades patients from enrolling. Our group has developed a catheter clearing procedure to minimize the extent of Contamination so that dosing and sampling from the CVL can ensue, promoting patient willingness to participate in phase I pediatric oncology trials. Objectives To develop a population pharmacokinetic model of actinomycin-D (AMD) in children with cancer incorporating expressions for Drug Contamination from PK samples obtained via indwelling CVLs and to evaluate the efficiency of a catheter clearing procedure in removing Contamination as well as the impact of Contamination on PK results. Methods A dataset of 199 AMD plasma concentration measurements from 36 patients (age 1.6–20.3 years) was analyzed using nonlinear mixed-effects modeling. Quantitative modeling approaches, including baseline Contamination model, covariate model, and catheter clearance model, were evaluated to describe catheter Contamination. Monte Carlo simulations mimicking a prospective study in children with cancer were performed to assess the performance of the final model and impact of catheter Contamination on PK reporting. Results The PK of AMD was best described by a linear 3-compartment model with first-order elimination. A baseline Contamination model including a Contamination factor proportional to the model-predicted concentration for samples obtained from central catheters was chosen as the most parsimonious and accurate among competing models. The final model parameters were allometrically scaled to a 70 kg person. The estimated mean parameter values were 11 L/h, 5.79, 24.2, 490 L, 17.7, and 42.8 L/h for total clearance, central volume of distribution, peripheral volume 1, peripheral volume 2, inter-compartmental clearance 1, and inter-compartmental clearance 2, respectively. The proportional Contamination factor was 19.3 % immediately post-Drug administration and decreased at a first-order rate of 0.0932 h^−1. Simulations precisely re-estimated kinetic parameters with catheter Contamination adjustment. Large uncertainty and poor estimation were observed when Contamination was ignored. Conclusions Drug Contamination from sampling catheter can impact AMD PK results and should be accounted for in the analysis. We provide a framework for evaluating catheter Contamination and guidance on adjustment in the PK model.
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Modeling and simulation approaches to evaluate pharmacokinetic sampling Contamination from central venous catheters in pediatric pharmacokinetic studies of actinomycin-D: a report from the children’s oncology group
Cancer Chemotherapy and Pharmacology, 2012Co-Authors: Alena Y. Z. Edwards, Jeffrey M. Skolnik, Erin Dombrowsky, Dimple Patel, Jeffrey S. BarrettAbstract:Background The binding of Drugs to catheters can be a source variation in dosing chemotherapeutics. Drug Contamination from the dosing central venous line (CVL) can impact the reporting of pharmacokinetic (PK) results and analysis. Peripheral venipuncture avoids binding complications from the CVL but dissuades patients from enrolling. Our group has developed a catheter clearing procedure to minimize the extent of Contamination so that dosing and sampling from the CVL can ensue, promoting patient willingness to participate in phase I pediatric oncology trials.
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Approaches to clear residual chemotherapeutics from indwelling catheters in children with cancer
Therapeutic Drug Monitoring, 2010Co-Authors: Jeffrey M. Skolnik, Jeffrey S. Barrett, Alena Y. Zhang, Peter C. AdamsonAbstract:Objectives: To develop a method for Drug dosing and pharmaco-kinetic (PK) sampling in children with cancer from a single indwelling central venous catheter that minimized Drug Contamination. Methods: A benchtop system was designed to simulate dosing and clearing actinomycin-D (AMD) and vincristine (VCR) from central venous catheters. The authors evaluated the effects of flush volume, composition and pH, timed Drug instillation, and number of blood-draw return cycles on residual Drug concentrations. A proof-of-principle study was conducted in three pediatric patients with cancer with paired PK samples obtained by both central and peripheral catheters. Results: Nearly complete removal of Drug from the catheter was obtained after five blood-draw return cycles consisting of 5 mL of whole blood. Residual concentration of AMD was 0.18 ± 0.02 ng/mL or 0.16% of the initial infusion concentration. VCR exhibited lower propensity for catheter adsorption than AMD with residual concentrations undetectable after three blood-draw return cycles. In patients in which the clearance procedure was used, higher Drug concentrations were generally observed from centrally cleared samples at most time points, but differences relative to peripherally obtained samples were not statistically significant for either AMD or VCR. Two of three patients had higher exposure for AMD based on PK samples obtained from central catheters, whereas exposure for VCR was similar for both sampling catheters in all patients. Conclusions: A reliable procedure to efficiently reduce AMD and VCR Contamination during PK sampling has been established and is currently being used in a PK study being conducted by the Children's Oncology Group.
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A method to perform pharmacokinetic sampling through a single lumen catheter following Drug administration
Cancer Research, 2006Co-Authors: Jeffrey M. Skolnik, Jeffrey S. Barrett, Dominique A. Paccaly, Peter C. AdamsonAbstract:3113 Introduction: The ability to use a single indwelling catheter for both dosing and sampling purposes can enhance enrollment in pharmacokinetic studies, especially in children. Objective: To develop a procedure for pharmacokinetic sampling from a single indwelling central catheter following Drug dosing. Material and Methods: An in vitro model system for Drug dosing through a central venous catheter using a variety of commercial catheters (Cook® and CR Bard®) was used for method development. A 3-way stopcock was fitted with both a waste and sample syringe, and was connected to the catheter via a needless access hub. Actinomycin-D (Act-D, 0.5 mg/mL) and vincristine (VCR, 1 mg/mL), two Drugs to be studied in an upcoming pharmacokinetic-pharmacodynamic study, were dosed through the catheter. Sampling occurred from Drug-free whole blood. The effect of flush volume and composition, as well as catheter type and lumen size, on residual Drug concentration was studied. Concentration profiles were fitted to simple exponential decay models, and were incorporated into models to explore the potential for clinically influential Contamination. Results: Limited residual Act-D and VCR concentrations were found in samples obtained after four rinse waste cycles interrupted by a 2 mL saline flush. Mean residual concentrations of Act-D were 0.6±0.6 ng/mL and of VCR were 0.3±0.4 ng/mL, at or below the limit of quantitation, reflecting a six log decrease from initial infusion concentration. Conclusion: We have developed a procedure to efficiently reduce Drug Contamination during pharmacokinetic sampling from a single lumen catheter. Further evaluation of in vivo validation is ongoing and the proposed configuration will soon be evaluated in a prospective clinical trial currently underway at our institution.
M. J. Struelens - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of pulsed-field gel electrophoresis and rep-PCR for the epidemiological analysis ofOchrobactrum anthropi strains
European Journal of Clinical Microbiology and Infectious Diseases, 1995Co-Authors: P. Dijck, M. Delmée, H. Ezzedine, A. Deplano, M. J. StruelensAbstract:Pulsed-field gel electrophoresis and polymerase chain reaction genome fingerprinting based on repetitive chromosomal sequences (rep-PCR) were used for typing 14 strains of Ochrobactrum anthropi . Six strains isolated during an outbreak of bacteraemia in patients who had received a contaminated rabbit anti-thymocyte globulin gave identical patterns by both techniques. Different patterns were found in sporadic and reference strains, except for one clinical isolate received from another hospital that showed the same pattern as the epidemic clone. This patient had also received rabbit anti-thymocyte globulin from the same source at the time of the outbreak. This study illustrates the advantages of genetic typing methods in terms of high typeability and discriminating power, even for rare pathogens. Furthermore, it highlights the need for interhospital communication for effective identification of common sources of outbreaks related to intrinsic Drug Contamination.
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Evaluation of pulsed-field gel electrophoresis and rep-PCR for the epidemiological analysis of Ochrobactrum anthropi strains
European Journal of Clinical Microbiology & Infectious Diseases, 1995Co-Authors: P. Dijck, M. Delmée, H. Ezzedine, A. Deplano, M. J. StruelensAbstract:Pulsed-field gel electrophoresis and polymerase chain reaction genome fingerprinting based on repetitive chromosomal sequences (rep-PCR) were used for typing 14 strains of Ochrobactrum anthropi. Six strains isolated during an outbreak of bacteraemia in patients who had received a contaminated rabbit anti-thymocyte globulin gave identical patterns by both techniques. Different patterns were found in sporadic and reference strains, except for one clinical isolate received from another hospital that showed the same pattern as the epidemic clone. This patient had also received rabbit anti-thymocyte globulin from the same source at the time of the outbreak. This study illustrates the advantages of genetic typing methods in terms of high typeability and discriminating power, even for rare pathogens. Furthermore, it highlights the need for interhospital communication for effective identification of common sources of outbreaks related to intrinsic Drug Contamination.
