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You-jin Jeon - One of the best experts on this subject based on the ideXlab platform.
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A sulfated polysaccharide of Ecklonia cava inhibits the growth of colon cancer cells by inducing apoptosis
EXCLI journal, 2015Co-Authors: Ginnae Ahn, Kil-Nam Kim, Soo-jin Heo, Ji-hyeok Lee, Seung-hong Lee, Wonwon Lee, Nalae Kang, Chang-bum Ahn, You-jin JeonAbstract:We investigated anticancer effects of the crude polysaccharides (CPs) isolated from Ecklonia cava enzymatic extracts using AMG, Viscozyme, Protamex, and Alcalase enzyme against a colon cancer cell line, CT26 cells. Among them, the CP of Protamex extract (PCP) contained the highest fucose and sulfated group contents and showed the highest growth inhibitory effect against CT-26 cells. In addition, PCP dose-dependently increased the formation of apoptotic body and the percentage of Sub-G1 DNA contents. Also, PCP activated caspase 9 and PARP as regulating the expressions of Bax and Bcl-2. Moreover, PPP2, a fraction purified from PCP showed the highest growth inhibitory effect against CT 26 cells with the increased fucose and sulfated group contents. The results demonstrate that the isolated SP containing plentiful fucose and sulfated group contents has the anticancer effect on colon cancer cells via regulation of Bcl-2/Bax signal pathway.
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Protective effect of polyphenol extracted from Ecklonia cava against ethanol induced oxidative damage in vitro and in zebrafish model
Journal of Functional Foods, 2014Co-Authors: Min Cheol Kang, Kil-Nam Kim, Ginnae Ahn, W. A J P Wijesinghe, Xiudong Yang, You-jin JeonAbstract:In the present study, the protective effects of polyphenol extract from Ecklonia cava (EPE) against ethanol-induced cell damage and oxidative stress were investigated in vitro and in vivo. E. cava was selected among twenty marine brown algae due to less cytotoxicity and higher cell viability against the ethanol-induced cell damage. The EPE extract showed the protective effect against the ethanol-mediated apoptosis, which was investigated via nuclear staining with Hoechst 33342 and flow cytometry. Furthermore, the treated EPE extract on zebrafish model was improved the ethanol induced survival rate and attenuated, oxidative stress and cell death. As well this result indicated that EPE extract rendered the protective effect of EPE extract against ethanol induced oxidative stress in vitro and zebrafish model.
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Potential use of ultrasound in antioxidant extraction from Ecklonia cava
ALGAE, 2013Co-Authors: Seung-hong Lee, Min Cheol Kang, Sang-ho Moon, Byong-tae Jeon, You-jin JeonAbstract:Water and methanolic extracts of Ecklonia cava, a marine brown alga, were prepared by ultrasonic extraction (UE) and conventional extraction (CE) methods. The radical-scavenging activity and the inhibitory effects against hydrogen peroxide (H2O2)-induced DNA damage of the extracts were investigated. All extracts prepared by CE exhibited higher total polyphenolic content than that in the extracts prepared by UE. Extraction yield and total phenolic content increased as the UE time increased. The radical-scavenging activities increased as the UE time increased. All extracts prepared by CE exhibited higher 1,1-diphenyl-2-pricrylhydrazyl (DPPH) and hydroxyl radical-scavenging activities than did those prepared by UE. Extracts prepared by UE showed stronger scavenging activities on alkyl radical and H2O2 than those prepared by CE did. Methanolic extract with UE 12 h (100MEU-12h) and methanolic extract with CE 24 h (100MEC-24h) were selected and evaluated by comet assay for their inhibitory effect against H2O2-induced DNA damage. 100MEU-12h showed slightly greater protective effect against H2O2-induced DNA damage than 100MEC-24h. Thus, UE can be effectively used as a seaweed extraction technique, and there is potential for scale-up of the extraction process.
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dieckol isolated from brown seaweed Ecklonia cava attenuates type іі diabetes in db db mouse model
Food and Chemical Toxicology, 2013Co-Authors: Min Cheol Kang, W. A J P Wijesinghe, Xiudong Yang, Sung-myung Kang, Seung-hong Lee, Byong-tae Jeon, Nalae Kang, Jaell Kim, Daeho Lee, You-jin JeonAbstract:In the present study, the attenuation of type ІІ diabetes by dieckol, a phlorotannin derivative isolated from brown seaweed, Ecklonia cava was investigated in C57BL/KsJ-db/db, a type ІІ diabetes mouse model. Dieckol was administered intraperitoneal injection at doses of 10 and 20 mg/kg body weight diabetes mice for 14 days. The blood glucose level, serum insulin level and body weight were significantly reduced in the dieckol administered group, compared to that of the saline administered group. Furthermore, reduced thiobarbituric acid reactive substraces (TBARS), as well as increased activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) in liver tissues were observed in the dieckol administered group. In addition, increased levels of the phosphorylation of AMPK and Akt were observed in the muscle tissues of the dieckol administered group in a Western blotting analysis. According to the findings of this study, it could be suggested that, dieckol can be developed as a therapeutic agent for type ІІ diabetes.
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Anti-inflammatory effect of fucoidan extracted from Ecklonia cava in zebrafish model.
Carbohydrate Polymers, 2013Co-Authors: Seung-hong Lee, YOUNG HEUN JEE, Jin-soo Kim, Misook Kim, Yoonhwa Jeong, You-jin JeonAbstract:Fucoidan extracted from Ecklonia cava had strong anti-inflammatory activities. However, the direct effects of fucoidan of E. cava on anti-inflammatory activities in vivo model remained to be determined. Therefore, the present study was designed to assess in vivo anti-inflammatory effect of fucoidan extracted from E. cava (ECF) using tail-cutting-induced and lipopolysaccharide (LPS)-stimulated zebrafish model. Treating zebrafish model with tail-cutting and LPS-treatment significantly increased the ROS and NO level. However, ECF inhibited this tail-cutting-induced and LPS-stimulated ROS and NO generation. These results show that ECF alleviated inflammation by inhibiting the ROS and NO generation induced by tail-cutting and LPS-treatment. In addition, ECF has a protective effect against the toxicity induced by LPS exposure in zebrafish embryos. This outcome could explain the potential anti-inflammatory activity of ECF, which might have a beneficial effect during the treatment of inflammatory diseases.
Ki-wan Lee - One of the best experts on this subject based on the ideXlab platform.
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anti inflammatory effects of enzymatic extract from Ecklonia cava on tpa induced ear skin edema
Food Science and Biotechnology, 2008Co-Authors: Ginnae Ahn, You-jin Jeon, Jae Woo Park, Ki-wan Lee, Eunjin Park, Dae Seung Kim, Taekyun Shin, Hochun Woo, YOUNG HEUN JEEAbstract:Anti-inflammatory potential of the enzymatic extract prepared by Kojizyme (ECK), a component of brown seaweeds Ecklonia cava (Alariaceae, Phaeophyta) in vivo was investigated. For the application of mouse ear edema model, 12-O-tetradecanoylphorbol acetate (TPA) was used, a topical inducer of a long-lasting inflammatory response. Our results demonstrated that ECK inhibited ear edema when topically applied to mouse ear skin. In histological evaluation, the inhibition activity of ECK on TPA-induced inflammation is similar to that of dexamethasone, although less strong. In addition, the mRNA expression levels of IL-1β, IFN-γ, TNF-α, and cyclooxygenase-2 (COX2) and the immunoreactivity to inducible nitric oxide synthase (iNOS) and COX2 expressed mainly in inflammatory cells were down-regulated by ECK. These results indicate that ECK has anti-inflammatory effects through the inhibition of Th1 cytokines and 2 inducers of inflammation in TPA-induced ear skin edema.
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antioxidant potential of Ecklonia cava on reactive oxygen species scavenging metal chelating reducing power and lipid peroxidation inhibition
Food Science and Technology International, 2006Co-Authors: Mahinda Senevirathne, Ki-wan Lee, Soo-hyun Kim, Nalin Siriwardhana, You-jin JeonAbstract:The antioxidative potential of different fractions (respective organic and aqueous fractions of n-hexane, chloroform and ethyl acetate) of 70% methanol extract of Ecklonia cava(a brown seaweed) was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide, hydroxyl radical, nitric oxide, ferrous ion chelating, reducing power and lipid peroxidation inhibition (conjugated diene hydroperoxide and thiobarbituric acid-reactive substances production) assays. The 70% methanol extract showed significant (p< 0.05) activities in all antioxidant assays and contained a high level of total phenolic content. It was observed that the level of hydrophilic phenolic content was higher than that of hydrophobics. Among those organic solvent fractions, ethyl acetate fraction exhibited significant activities due to the highest level of total phenolic content and their IC50 values were 0.013mg/mL, 0.009mg/mL and 0.33mg/mL in DPPH, hydrogen peroxide and nitric oxide radical inhibition, respectivel...
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Antioxidant potential of Ecklonia cava on reactive oxygen species scavenging, metal chelating, reducing power and lipid peroxidation inhibition
2006Co-Authors: Mahinda Senevirathne, Soo-hyun Kim, Nalin Siriwardhana, Ki-wan LeeAbstract:The antioxidative potential of different fractions (respective organic and aqueous fractions of n-hexane, chloroform and ethyl acetate) of 70 % methanol extract of Ecklonia cava (a brown seaweed) was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide, hydroxyl radical, nitric oxide, ferrous ion chelating, reducing power and lipid peroxidation inhibition (conjugated diene hydroper-oxide and thiobarbituric acid-reactive substances production) assays. The 70 % methanol extract showed significant (p<0.05) activities in all antioxidant assays and contained a high level of total phenolic content. It was observed that the level of hydrophilic phenolic content was higher than that of hydrophobics. Among those organic solvent fractions, ethyl acetate fraction exhibited significant activities due to the highest level of total phenolic content and their IC50 values were 0.013mg/mL, 0.009mg/mL and 0.33mg/mL in DPPH, hydrogen peroxide and nitric oxide radical inhibition, respectively. These activities were superior to those of a commercial synthetic and natural antioxidants tested. The aqueous chloroform and ethyl acetate fractions also exhibited significant (p<0.05) activities in reactive oxygen species (ROS) scavenging and metal chelating, attributed to the high amount of hydrophilic phenolics. Moreover, E. cava extracts showed strong reducing power and a notable capacity to suppress lipid peroxidation. Key Words: Ecklonia cava, brown seaweed, antioxidant, lipid peroxidation, reactive oxygen specie
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Antioxidant Effect of Enzymatic Hydrolyzate from a Kelp, Ecklonia cava
ALGAE, 2003Co-Authors: Soo-jin Heo, You-jin Jeon, Jehee Lee, Hung-tae Kim, Ki-wan LeeAbstract:The potential antioxidative activity of water-soluble enzymatic hydrolyzates from a kelp, Ecklonia cava was evaluated by free radical scavenging and lipid peroxidation assays. To prepare water-soluble hydrolyzates from E. cava the seaweed was enzymatically hydrolyzed by five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase). Among all the hydrolyzates, Celluclast hydrolyzate effectively scavenged free radicals released from DPPH (1,1-diphenyl-2- pricrylhydrazyl) and recorded around 73% scavenging activity at the concentration of 4 mg . This hydrolyzate was thermally stable and DPPH radical scavenging activity remained 80% or higher at heating temperatures of 40 and 60 up to 12 h and around 80% at 100 up to 8 h. AMG and Ultraflo hydrolyzate inhibited the lipid peroxidation of fish oil as that of -tocopherol. These results suggested that an enzymatic extraction will be an effective way for the production of a potential antioxidant from seaweeds.
Min Cheol Kang - One of the best experts on this subject based on the ideXlab platform.
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Protective effect of polyphenol extracted from Ecklonia cava against ethanol induced oxidative damage in vitro and in zebrafish model
Journal of Functional Foods, 2014Co-Authors: Min Cheol Kang, Kil-Nam Kim, Ginnae Ahn, W. A J P Wijesinghe, Xiudong Yang, You-jin JeonAbstract:In the present study, the protective effects of polyphenol extract from Ecklonia cava (EPE) against ethanol-induced cell damage and oxidative stress were investigated in vitro and in vivo. E. cava was selected among twenty marine brown algae due to less cytotoxicity and higher cell viability against the ethanol-induced cell damage. The EPE extract showed the protective effect against the ethanol-mediated apoptosis, which was investigated via nuclear staining with Hoechst 33342 and flow cytometry. Furthermore, the treated EPE extract on zebrafish model was improved the ethanol induced survival rate and attenuated, oxidative stress and cell death. As well this result indicated that EPE extract rendered the protective effect of EPE extract against ethanol induced oxidative stress in vitro and zebrafish model.
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Potential use of ultrasound in antioxidant extraction from Ecklonia cava
ALGAE, 2013Co-Authors: Seung-hong Lee, Min Cheol Kang, Sang-ho Moon, Byong-tae Jeon, You-jin JeonAbstract:Water and methanolic extracts of Ecklonia cava, a marine brown alga, were prepared by ultrasonic extraction (UE) and conventional extraction (CE) methods. The radical-scavenging activity and the inhibitory effects against hydrogen peroxide (H2O2)-induced DNA damage of the extracts were investigated. All extracts prepared by CE exhibited higher total polyphenolic content than that in the extracts prepared by UE. Extraction yield and total phenolic content increased as the UE time increased. The radical-scavenging activities increased as the UE time increased. All extracts prepared by CE exhibited higher 1,1-diphenyl-2-pricrylhydrazyl (DPPH) and hydroxyl radical-scavenging activities than did those prepared by UE. Extracts prepared by UE showed stronger scavenging activities on alkyl radical and H2O2 than those prepared by CE did. Methanolic extract with UE 12 h (100MEU-12h) and methanolic extract with CE 24 h (100MEC-24h) were selected and evaluated by comet assay for their inhibitory effect against H2O2-induced DNA damage. 100MEU-12h showed slightly greater protective effect against H2O2-induced DNA damage than 100MEC-24h. Thus, UE can be effectively used as a seaweed extraction technique, and there is potential for scale-up of the extraction process.
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dieckol isolated from brown seaweed Ecklonia cava attenuates type іі diabetes in db db mouse model
Food and Chemical Toxicology, 2013Co-Authors: Min Cheol Kang, W. A J P Wijesinghe, Xiudong Yang, Sung-myung Kang, Seung-hong Lee, Byong-tae Jeon, Nalae Kang, Jaell Kim, Daeho Lee, You-jin JeonAbstract:In the present study, the attenuation of type ІІ diabetes by dieckol, a phlorotannin derivative isolated from brown seaweed, Ecklonia cava was investigated in C57BL/KsJ-db/db, a type ІІ diabetes mouse model. Dieckol was administered intraperitoneal injection at doses of 10 and 20 mg/kg body weight diabetes mice for 14 days. The blood glucose level, serum insulin level and body weight were significantly reduced in the dieckol administered group, compared to that of the saline administered group. Furthermore, reduced thiobarbituric acid reactive substraces (TBARS), as well as increased activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) in liver tissues were observed in the dieckol administered group. In addition, increased levels of the phosphorylation of AMPK and Akt were observed in the muscle tissues of the dieckol administered group in a Western blotting analysis. According to the findings of this study, it could be suggested that, dieckol can be developed as a therapeutic agent for type ІІ diabetes.
Ginnae Ahn - One of the best experts on this subject based on the ideXlab platform.
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Dieckol, a phlorotannin of Ecklonia cava, suppresses IgE-mediated mast cell activation and passive cutaneous anaphylactic reaction.
Experimental Dermatology, 2015Co-Authors: Ginnae Ahn, Yosuke Amagai, Akira Matsuda, Sung-myung Kang, Wonwoo Lee, Kyungsook Jung, Kumiko Oida, Hyosun Jang, Saori Ishizaka, Kenshiro MatsudaAbstract:Keywords: Dieckol; Ecklonia cava ; mast cell; passive cutaneous anaphylactic reaction
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A sulfated polysaccharide of Ecklonia cava inhibits the growth of colon cancer cells by inducing apoptosis
EXCLI journal, 2015Co-Authors: Ginnae Ahn, Kil-Nam Kim, Soo-jin Heo, Ji-hyeok Lee, Seung-hong Lee, Wonwon Lee, Nalae Kang, Chang-bum Ahn, You-jin JeonAbstract:We investigated anticancer effects of the crude polysaccharides (CPs) isolated from Ecklonia cava enzymatic extracts using AMG, Viscozyme, Protamex, and Alcalase enzyme against a colon cancer cell line, CT26 cells. Among them, the CP of Protamex extract (PCP) contained the highest fucose and sulfated group contents and showed the highest growth inhibitory effect against CT-26 cells. In addition, PCP dose-dependently increased the formation of apoptotic body and the percentage of Sub-G1 DNA contents. Also, PCP activated caspase 9 and PARP as regulating the expressions of Bax and Bcl-2. Moreover, PPP2, a fraction purified from PCP showed the highest growth inhibitory effect against CT 26 cells with the increased fucose and sulfated group contents. The results demonstrate that the isolated SP containing plentiful fucose and sulfated group contents has the anticancer effect on colon cancer cells via regulation of Bcl-2/Bax signal pathway.
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Protective effect of polyphenol extracted from Ecklonia cava against ethanol induced oxidative damage in vitro and in zebrafish model
Journal of Functional Foods, 2014Co-Authors: Min Cheol Kang, Kil-Nam Kim, Ginnae Ahn, W. A J P Wijesinghe, Xiudong Yang, You-jin JeonAbstract:In the present study, the protective effects of polyphenol extract from Ecklonia cava (EPE) against ethanol-induced cell damage and oxidative stress were investigated in vitro and in vivo. E. cava was selected among twenty marine brown algae due to less cytotoxicity and higher cell viability against the ethanol-induced cell damage. The EPE extract showed the protective effect against the ethanol-mediated apoptosis, which was investigated via nuclear staining with Hoechst 33342 and flow cytometry. Furthermore, the treated EPE extract on zebrafish model was improved the ethanol induced survival rate and attenuated, oxidative stress and cell death. As well this result indicated that EPE extract rendered the protective effect of EPE extract against ethanol induced oxidative stress in vitro and zebrafish model.
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Radio-protective effect of polysaccharides isolated from Lactobacillus brevis-fermented Ecklonia cava.
International Journal of Biological Macromolecules, 2013Co-Authors: Wonwoo Lee, Ginnae Ahn, YOUNG HEUN JEE, W. A J P Wijesinghe, Bae-jin Lee, Daekyung Kim, Hye-mi Yang, Young Mog Kim, Sun Joo Park, You-jin JeonAbstract:Abstract We investigated the radioprotective effects of a polysaccharide isolated from enzymatic extracts of Ecklonia cava ( E. cava ) fermented by fungi and bacteria. We identified that the aqueous extract of the Lactobacillus brevis -fermented E. cava especially showed the highest proliferation effect. In addition, the enzymatic extract prepared by enzyme-assisted extraction using Viscozyme (VLFE) significantly increased cell proliferation. Further study indicated that the polysaccharides isolated from the >30 kDa fraction of VLFE (VLFEP) significantly enhanced survival and proliferation effects in γ-ray-irradiated cells. Also, VLFEP markedly reduced the DNA damage, production of reactive oxygen species, and the percentage of Sub-G 1 DNA contents caused by γ-ray-irradiation. Moreover, VLFEP modulated the expression levels of p53, Bax, and Bcl-2 via inhibition of IκBα degradation and phosphorylation and NFκB p65 translocation into nuclei. These results demonstrate that VLFEP has radioprotective properties including the modulation of apoptosis via the inhibition of the NFκB signaling pathway.
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Phloroglucinol (PG) purified from Ecklonia cava attenuates radiation-induced apoptosis in blood lymphocytes and splenocytes.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 2011Co-Authors: Suk-Jae Park, Ginnae Ahn, Nam Ho Lee, You-jin Jeon, Jae Woo Park, YOUNG HEUN JEEAbstract:Phloroglucinol (PG), a polyphenol compound of Eckloniacava known as brown algae abundant in Jeju island, has been proposed to exert the antioxidative and cytoprotective effects against oxidative stress. In this study, we confirmed that PG protected mice from damages caused by ionizing radiation and investigated its protection mechanism in detail. The result showed that PG increased the viability of splenocytes without cytotoxicity. Also, PG significantly enhanced the proliferation of splenocytes by limiting the increment of sub-G(1) DNA contents via the inhibition of reactive oxygen species production in 2 Gy-irradiated splenocytes. In addition, PG significantly decreased DNA damage and the number of apoptotic fragments in lymphocytes against oxidative stress. Also, PG increased the counts of endogenous spleen CFUs, compared with only ionizing radiation-irradiated mice. These results demonstrate the multi-faceted protection mechanisms of PG in mice against oxidative stress caused by ionizing radiation, providing the benefit of inhibiting apoptosis and strengthening hematopoiesis.
W. A J P Wijesinghe - One of the best experts on this subject based on the ideXlab platform.
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Protective effect of polyphenol extracted from Ecklonia cava against ethanol induced oxidative damage in vitro and in zebrafish model
Journal of Functional Foods, 2014Co-Authors: Min Cheol Kang, Kil-Nam Kim, Ginnae Ahn, W. A J P Wijesinghe, Xiudong Yang, You-jin JeonAbstract:In the present study, the protective effects of polyphenol extract from Ecklonia cava (EPE) against ethanol-induced cell damage and oxidative stress were investigated in vitro and in vivo. E. cava was selected among twenty marine brown algae due to less cytotoxicity and higher cell viability against the ethanol-induced cell damage. The EPE extract showed the protective effect against the ethanol-mediated apoptosis, which was investigated via nuclear staining with Hoechst 33342 and flow cytometry. Furthermore, the treated EPE extract on zebrafish model was improved the ethanol induced survival rate and attenuated, oxidative stress and cell death. As well this result indicated that EPE extract rendered the protective effect of EPE extract against ethanol induced oxidative stress in vitro and zebrafish model.
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dieckol isolated from brown seaweed Ecklonia cava attenuates type іі diabetes in db db mouse model
Food and Chemical Toxicology, 2013Co-Authors: Min Cheol Kang, W. A J P Wijesinghe, Xiudong Yang, Sung-myung Kang, Seung-hong Lee, Byong-tae Jeon, Nalae Kang, Jaell Kim, Daeho Lee, You-jin JeonAbstract:In the present study, the attenuation of type ІІ diabetes by dieckol, a phlorotannin derivative isolated from brown seaweed, Ecklonia cava was investigated in C57BL/KsJ-db/db, a type ІІ diabetes mouse model. Dieckol was administered intraperitoneal injection at doses of 10 and 20 mg/kg body weight diabetes mice for 14 days. The blood glucose level, serum insulin level and body weight were significantly reduced in the dieckol administered group, compared to that of the saline administered group. Furthermore, reduced thiobarbituric acid reactive substraces (TBARS), as well as increased activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) in liver tissues were observed in the dieckol administered group. In addition, increased levels of the phosphorylation of AMPK and Akt were observed in the muscle tissues of the dieckol administered group in a Western blotting analysis. According to the findings of this study, it could be suggested that, dieckol can be developed as a therapeutic agent for type ІІ diabetes.
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Radio-protective effect of polysaccharides isolated from Lactobacillus brevis-fermented Ecklonia cava.
International Journal of Biological Macromolecules, 2013Co-Authors: Wonwoo Lee, Ginnae Ahn, YOUNG HEUN JEE, W. A J P Wijesinghe, Bae-jin Lee, Daekyung Kim, Hye-mi Yang, Young Mog Kim, Sun Joo Park, You-jin JeonAbstract:Abstract We investigated the radioprotective effects of a polysaccharide isolated from enzymatic extracts of Ecklonia cava ( E. cava ) fermented by fungi and bacteria. We identified that the aqueous extract of the Lactobacillus brevis -fermented E. cava especially showed the highest proliferation effect. In addition, the enzymatic extract prepared by enzyme-assisted extraction using Viscozyme (VLFE) significantly increased cell proliferation. Further study indicated that the polysaccharides isolated from the >30 kDa fraction of VLFE (VLFEP) significantly enhanced survival and proliferation effects in γ-ray-irradiated cells. Also, VLFEP markedly reduced the DNA damage, production of reactive oxygen species, and the percentage of Sub-G 1 DNA contents caused by γ-ray-irradiation. Moreover, VLFEP modulated the expression levels of p53, Bax, and Bcl-2 via inhibition of IκBα degradation and phosphorylation and NFκB p65 translocation into nuclei. These results demonstrate that VLFEP has radioprotective properties including the modulation of apoptosis via the inhibition of the NFκB signaling pathway.
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Exploiting biological activities of brown seaweed Ecklonia cava for potential industrial applications: a review
International Journal of Food Sciences and Nutrition, 2011Co-Authors: W. A J P Wijesinghe, You-jin JeonAbstract:Seaweeds are rich in vitamins, minerals, dietary fibres, proteins, polysaccharides and various functional polyphenols. Many researchers have focused on brown algae as a potential source of bioactive materials in the past few decades. Ecklonia cava is a brown seaweed that is abundant in the subtidal regions of Jeju Island in the Republic of Korea. This seaweed attracted extensive interest due to its multiple biological activities. E. cava has been identified as a potential producer of wide spectrum of natural substances such as carotenoids, fucoidans and phlorotannins showing different biological activities in vital industrial applications including pharmaceutical, nutraceutical, cosmeceutical and functional food. This review focuses on biological activities of the brown seaweed E. cava based on latest research results, including antioxidant, anticoagulative, antimicrobial, antihuman immunodeficiency virus, anti-inflammatory, immunomodulatory, antimutagenic, antitumour and anticancer effects. The facts sum...
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Effect of phlorotannins isolated from Ecklonia cava on angiotensin I-converting enzyme (ACE) inhibitory activity
Nutrition Research and Practice, 2011Co-Authors: W. A J P Wijesinghe, You-jin JeonAbstract:Inhibition of angiotensin I-converting enzyme (ACE) activity is the most common mechanism underlying the lowering of blood pressure. In the present study, five organic extracts of a marine brown seaweed Ecklonia cava were prepared by using ethanol, ethyl acetate, chloroform, hexane, and diethyl ether as solvents, which were then tested for their potential ACE inhibitory activities. Ethanol extract showed the strongest ACE inhibitory activity with an IC50 value of 0.96 mg/ml. Five kinds of phlorotannins, phloroglucinol, triphlorethol-A, eckol, dieckol, and eckstolonol, were isolated from ethanol extract of E. cava, which exhibited potential ACE inhibition. Dieckol was the most potent ACE inhibitor and was found to be a non-competitive inhibitor against ACE according to Lineweaver-Burk plots. Dieckol had an inducible effect on the production of NO in EAhy926 cells without having cytotoxic effect. The results of this study indicate that E. cava could be a potential source of phlorotannins with ACE inhibitory activity for utilization in production of functional foods.
