The Experts below are selected from a list of 2907 Experts worldwide ranked by ideXlab platform
Jiro Maegawa - One of the best experts on this subject based on the ideXlab platform.
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autotransplantation of monkey ear perichondrium derived progenitor cells for Cartilage reconstruction
Cell Transplantation, 2016Co-Authors: Shintaro Kagimoto, Takanori Takebe, Yuichiro Yabuki, Shinji Kobayashi, Toshimasa Uemura, Ayaka Hori, Koichi Hirotomi, Taro Mikami, Jiro MaegawaAbstract:We recently developed a promising regenerative method based on the xenotransplantation of human Cartilage progenitor cells, demonstrating self-renewing Elastic Cartilage reconstruction with expected long-term tissue restoration. However, it remains unclear whether autotransplantation of Cartilage progenitors may work by a similar principle in immunocompetent individuals. We used a nonhuman primate (monkey) model to assess the safety and efficacy of our regenerative approach because the model shares characteristics with humans in terms of biological functions, including anatomical features. First, we identified the expandable and multipotent progenitor population from monkey ear perichondrium and succeeded in inducing chondrocyte differentiation in vitro. Second, in vivo transplanted progenitor cells were capable of reconstructing Elastic Cartilage by xenotransplantation into an immunodeficient mouse. Finally, the autologous monkey progenitor cells were transplanted into the subcutaneous region of a craniofacial section and developed mature Elastic Cartilage of their own 3 months after transplantation. Furthermore, we attempted to develop a clinically relevant, noninvasive monitoring method using magnetic resonance imaging (MRI). Collectively, this report shows that the autologous transplantation of Cartilage progenitors is potentially effective for reconstructing Elastic Cartilage. This principle will be invaluable for repairing craniofacial injuries and abnormalities in the context of plastic and reconstructive surgery.
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Transient vascularization of transplanted human adult–derived progenitors promotes self-organizing Cartilage
The Journal of clinical investigation, 2014Co-Authors: Takanori Takebe, Mitsuru Mizuno, Shinji Kobayashi, Ayaka Hori, Hiromu Suzuki, Yu-min Chang, Emi Yoshizawa, Masaki Kimura, Jun Asano, Jiro MaegawaAbstract:Millions of patients worldwide are affected by craniofacial deformations caused by congenital defects or trauma. Current surgical interventions have limited therapeutic outcomes; therefore, methods that would allow Cartilage restoration are of great interest. A number of studies on embryonic limb development have shown that chondrogenesis is initiated by cellular condensation, during which mesenchymal progenitors aggregate and form 3D structures. Here, we demonstrated efficient regeneration of avascular Elastic Cartilage from in vitro-grown mesenchymal condensation, which recapitulated the early stages of chondrogenesis, including transient vascularization. After transplantation of vascularized condensed progenitors into immunodeficient mice, we used an intravital imaging approach to follow Cartilage maturation. We determined that endothelial cells are present inside rudimentary Cartilage (mesenchymal condensation) prior to Cartilage maturation. Recreation of endothelial interactions in culture enabled a recently identified population of adult Elastic Cartilage progenitors to generate mesenchymal condensation in a self-driven manner, without requiring the support of exogenous inductive factors or scaffold materials. Moreover, the culture-grown 3D condensed adult-derived progenitors were amenable to storage via simple freezing methods and efficiently reconstructed 3D Elastic Cartilage upon transplantation. Together, our results indicate that transplantation of endothelialized and condensed progenitors represents a promising approach to realizing a regenerative medicine treatment for craniofacial deformations.
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brief report reconstruction of joint hyaline Cartilage by autologous progenitor cells derived from ear Elastic Cartilage
Stem Cells, 2014Co-Authors: Mitsuru Mizuno, Takanori Takebe, Yuichiro Yabuki, Takahisa Matsuzaki, Lee Jeong Ik, Hiroshi Yoshikawa, Shinji Kobayashi, Seiichiro Nakabayashi, Jiro MaegawaAbstract:In healthy joints, hyaline Cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human Cartilage progenitor cells from ear Elastic Cartilage. Human Cartilage progenitor cells have high chondrogenic and proliferative potential to form Elastic Cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived Cartilage progenitor cells can be used to reconstruct hyaline Cartilage, which has different mechanical and histological properties from Elastic Cartilage. In our efforts to develop foundational technologies for joint hyaline Cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint Cartilage damage, transplanted ear-derived autologous Cartilage progenitor cells. The reconstructed Cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline Cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear Cartilage and equal to those of joint hyaline Cartilage. This study suggested that joint hyaline Cartilage was reconstructed from ear-derived Cartilage progenitor cells. It also demonstrated that ear-derived Cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline Cartilage in patients with degenerative arthropathies. Stem Cells 2014;32:816–821
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brief report reconstruction of joint hyaline Cartilage by autologous progenitor cells derived from ear Elastic Cartilage
Stem Cells, 2014Co-Authors: Mitsuru Mizuno, Takanori Takebe, Yuichiro Yabuki, Takahisa Matsuzaki, Hiroshi Yoshikawa, Shinji Kobayashi, Seiichiro Nakabayashi, Hiroomi Kan, Jiro MaegawaAbstract:In healthy joints, hyaline Cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human Cartilage progenitor cells from ear Elastic Cartilage. Human Cartilage progenitor cells have high chondrogenic and proliferative potential to form Elastic Cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived Cartilage progenitor cells can be used to reconstruct hyaline Cartilage, which has different mechanical and histological properties from Elastic Cartilage. In our efforts to develop foundational technologies for joint hyaline Cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint Cartilage damage, transplanted ear-derived autologous Cartilage progenitor cells. The reconstructed Cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline Cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear Cartilage and equal to those of joint hyaline Cartilage. This study suggested that joint hyaline Cartilage was reconstructed from ear-derived Cartilage progenitor cells. It also demonstrated that ear-derived Cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline Cartilage in patients with degenerative arthropathies.
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Human Elastic Cartilage engineering from Cartilage progenitor cells using rotating wall vessel bioreactor.
Transplantation Proceedings, 2012Co-Authors: Takanori Takebe, Takuro Adegawa, Yuichiro Yabuki, J. Tanaka, Shinji Kobayashi, Tomohiko Yoshioka, M. Mizuno, H. Suzuki, Jiro MaegawaAbstract:Abstract Transplantation of bioengineered Elastic Cartilage is considered to be a promising approach for patients with craniofacial defects. We have previously shown that human ear perichondrium harbors a population of Cartilage progenitor cells (CPCs). The aim of this study was to examine the use of a rotating wall vessel (RWV) bioreactor for CPCs to engineer 3-D Elastic Cartilage in vitro. Human CPCs isolated from ear perichondrium were expanded and differentiated into chondrocytes under 2-D culture conditions. Fully differentiated CPCs were seeded into recently developed pC-HAp/ChS (porous material consisted of collagen, hydroxyapatite, and chondroitinsulfate) scaffolds and 3-D cultivated utilizing a RWV bioreactor. 3-D engineered constructs appeared shiny with a yellowish, Cartilage-like morphology. The shape of the molded scaffold was maintained after RWV cultivation. Hematoxylin and eosin staining showed engraftment of CPCs inside pC-HAp/ChS. Alcian blue and Elastica Van Gieson staining showed of proteoglycan and Elastic fibers, which are unique extracellular matrices of Elastic Cartilage. Thus, human CPCs formed Elastic Cartilage-like tissue after 3-D cultivation in a RWV bioreactor. These techniques may assist future efforts to reconstruct complicate structures composed of Elastic Cartilage in vitro.
Shinji Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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Development of a Method for Scaffold-Free Elastic Cartilage Creation.
International journal of molecular sciences, 2020Co-Authors: Masahiro Enomura, Yuichiro Yabuki, Shinji Kobayashi, Soichiro Murata, Yuri Terado, Maiko Tanaka, Takayoshi Oba, Shintaro Kagimoto, Kenichi Morita, Toshimasa UemuraAbstract:Microtia is a congenital aplasia of the auricular Cartilage. Conventionally, autologous costal Cartilage grafts are collected and shaped for transplantation. However, in this method, excessive invasion occurs due to limitations in the costal Cartilage collection. Due to deformation over time after transplantation of the shaped graft, problems with long-term morphological maintenance exist. Additionally, the lack of Elasticity with costal Cartilage grafts is worth mentioning, as costal Cartilage is a type of hyaline Cartilage. Medical plastic materials have been transplanted as alternatives to costal Cartilage, but transplant rejection and deformation over time are inevitable. It is imperative to create tissues for transplantation using cells of biological origin. Hence, Cartilage tissues were developed using a biodegradable scaffold material. However, such materials suffer from transplant rejection and biodegradation, causing the transplanted Cartilage tissue to deform due to a lack of Elasticity. To address this problem, we established a method for creating Elastic Cartilage tissue for transplantation with autologous cells without using scaffold materials. Chondrocyte progenitor cells were collected from perichondrial tissue of the ear Cartilage. By using a multilayer culture and a three-dimensional rotating suspension culture vessel system, we succeeded in creating scaffold-free Elastic Cartilage from Cartilage progenitor cells.
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autotransplantation of monkey ear perichondrium derived progenitor cells for Cartilage reconstruction
Cell Transplantation, 2016Co-Authors: Shintaro Kagimoto, Takanori Takebe, Yuichiro Yabuki, Shinji Kobayashi, Toshimasa Uemura, Ayaka Hori, Koichi Hirotomi, Taro Mikami, Jiro MaegawaAbstract:We recently developed a promising regenerative method based on the xenotransplantation of human Cartilage progenitor cells, demonstrating self-renewing Elastic Cartilage reconstruction with expected long-term tissue restoration. However, it remains unclear whether autotransplantation of Cartilage progenitors may work by a similar principle in immunocompetent individuals. We used a nonhuman primate (monkey) model to assess the safety and efficacy of our regenerative approach because the model shares characteristics with humans in terms of biological functions, including anatomical features. First, we identified the expandable and multipotent progenitor population from monkey ear perichondrium and succeeded in inducing chondrocyte differentiation in vitro. Second, in vivo transplanted progenitor cells were capable of reconstructing Elastic Cartilage by xenotransplantation into an immunodeficient mouse. Finally, the autologous monkey progenitor cells were transplanted into the subcutaneous region of a craniofacial section and developed mature Elastic Cartilage of their own 3 months after transplantation. Furthermore, we attempted to develop a clinically relevant, noninvasive monitoring method using magnetic resonance imaging (MRI). Collectively, this report shows that the autologous transplantation of Cartilage progenitors is potentially effective for reconstructing Elastic Cartilage. This principle will be invaluable for repairing craniofacial injuries and abnormalities in the context of plastic and reconstructive surgery.
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Transient vascularization of transplanted human adult–derived progenitors promotes self-organizing Cartilage
The Journal of clinical investigation, 2014Co-Authors: Takanori Takebe, Mitsuru Mizuno, Shinji Kobayashi, Ayaka Hori, Hiromu Suzuki, Yu-min Chang, Emi Yoshizawa, Masaki Kimura, Jun Asano, Jiro MaegawaAbstract:Millions of patients worldwide are affected by craniofacial deformations caused by congenital defects or trauma. Current surgical interventions have limited therapeutic outcomes; therefore, methods that would allow Cartilage restoration are of great interest. A number of studies on embryonic limb development have shown that chondrogenesis is initiated by cellular condensation, during which mesenchymal progenitors aggregate and form 3D structures. Here, we demonstrated efficient regeneration of avascular Elastic Cartilage from in vitro-grown mesenchymal condensation, which recapitulated the early stages of chondrogenesis, including transient vascularization. After transplantation of vascularized condensed progenitors into immunodeficient mice, we used an intravital imaging approach to follow Cartilage maturation. We determined that endothelial cells are present inside rudimentary Cartilage (mesenchymal condensation) prior to Cartilage maturation. Recreation of endothelial interactions in culture enabled a recently identified population of adult Elastic Cartilage progenitors to generate mesenchymal condensation in a self-driven manner, without requiring the support of exogenous inductive factors or scaffold materials. Moreover, the culture-grown 3D condensed adult-derived progenitors were amenable to storage via simple freezing methods and efficiently reconstructed 3D Elastic Cartilage upon transplantation. Together, our results indicate that transplantation of endothelialized and condensed progenitors represents a promising approach to realizing a regenerative medicine treatment for craniofacial deformations.
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Elastic Cartilage reconstruction by transplantation of cultured hyaline Cartilage-derived chondrocytes.
Transplantation Proceedings, 2014Co-Authors: M. Mizuno, Takanori Takebe, Y.h. Jo, Shinji Kobayashi, Masayuki Masutani, S. Kimura, Hideki TaniguchiAbstract:Abstract Current surgical intervention of craniofacial defects caused by injuries or abnormalities uses reconstructive materials, such as autologous Cartilage grafts. Transplantation of autologous tissues, however, places a significant invasiveness on patients, and many efforts have been made for establishing an alternative graft. Recently, we and others have shown the potential use of reconstructed Elastic Cartilage from ear-derived chondrocytes or progenitors with the unique Elastic properties. Here, we examined the differentiation potential of canine joint Cartilage–derived chondrocytes into Elastic Cartilage for expanding the cell sources, such as hyaline Cartilage. Articular chondrocytes are isolated from canine joint, cultivated, and compared regarding characteristic differences with auricular chondrocytes, including proliferation rates, gene expression, extracellular matrix production, and Cartilage reconstruction capability after transplantation. Canine articular chondrocytes proliferated less robustly than auricular chondrocytes, but there was no significant difference in the amount of sulfated glycosaminoglycan produced from redifferentiated chondrocytes. Furthermore, in vitro expanded and redifferentiated articular chondrocytes have been shown to reconstruct Elastic Cartilage on transplantation that has histologic characteristics distinct from hyaline Cartilage. Taken together, cultured hyaline Cartilage–derived chondrocytes are a possible cell source for Elastic Cartilage reconstruction.
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brief report reconstruction of joint hyaline Cartilage by autologous progenitor cells derived from ear Elastic Cartilage
Stem Cells, 2014Co-Authors: Mitsuru Mizuno, Takanori Takebe, Yuichiro Yabuki, Takahisa Matsuzaki, Lee Jeong Ik, Hiroshi Yoshikawa, Shinji Kobayashi, Seiichiro Nakabayashi, Jiro MaegawaAbstract:In healthy joints, hyaline Cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human Cartilage progenitor cells from ear Elastic Cartilage. Human Cartilage progenitor cells have high chondrogenic and proliferative potential to form Elastic Cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived Cartilage progenitor cells can be used to reconstruct hyaline Cartilage, which has different mechanical and histological properties from Elastic Cartilage. In our efforts to develop foundational technologies for joint hyaline Cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint Cartilage damage, transplanted ear-derived autologous Cartilage progenitor cells. The reconstructed Cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline Cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear Cartilage and equal to those of joint hyaline Cartilage. This study suggested that joint hyaline Cartilage was reconstructed from ear-derived Cartilage progenitor cells. It also demonstrated that ear-derived Cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline Cartilage in patients with degenerative arthropathies. Stem Cells 2014;32:816–821
Takanori Takebe - One of the best experts on this subject based on the ideXlab platform.
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autotransplantation of monkey ear perichondrium derived progenitor cells for Cartilage reconstruction
Cell Transplantation, 2016Co-Authors: Shintaro Kagimoto, Takanori Takebe, Yuichiro Yabuki, Shinji Kobayashi, Toshimasa Uemura, Ayaka Hori, Koichi Hirotomi, Taro Mikami, Jiro MaegawaAbstract:We recently developed a promising regenerative method based on the xenotransplantation of human Cartilage progenitor cells, demonstrating self-renewing Elastic Cartilage reconstruction with expected long-term tissue restoration. However, it remains unclear whether autotransplantation of Cartilage progenitors may work by a similar principle in immunocompetent individuals. We used a nonhuman primate (monkey) model to assess the safety and efficacy of our regenerative approach because the model shares characteristics with humans in terms of biological functions, including anatomical features. First, we identified the expandable and multipotent progenitor population from monkey ear perichondrium and succeeded in inducing chondrocyte differentiation in vitro. Second, in vivo transplanted progenitor cells were capable of reconstructing Elastic Cartilage by xenotransplantation into an immunodeficient mouse. Finally, the autologous monkey progenitor cells were transplanted into the subcutaneous region of a craniofacial section and developed mature Elastic Cartilage of their own 3 months after transplantation. Furthermore, we attempted to develop a clinically relevant, noninvasive monitoring method using magnetic resonance imaging (MRI). Collectively, this report shows that the autologous transplantation of Cartilage progenitors is potentially effective for reconstructing Elastic Cartilage. This principle will be invaluable for repairing craniofacial injuries and abnormalities in the context of plastic and reconstructive surgery.
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Transient vascularization of transplanted human adult–derived progenitors promotes self-organizing Cartilage
The Journal of clinical investigation, 2014Co-Authors: Takanori Takebe, Mitsuru Mizuno, Shinji Kobayashi, Ayaka Hori, Hiromu Suzuki, Yu-min Chang, Emi Yoshizawa, Masaki Kimura, Jun Asano, Jiro MaegawaAbstract:Millions of patients worldwide are affected by craniofacial deformations caused by congenital defects or trauma. Current surgical interventions have limited therapeutic outcomes; therefore, methods that would allow Cartilage restoration are of great interest. A number of studies on embryonic limb development have shown that chondrogenesis is initiated by cellular condensation, during which mesenchymal progenitors aggregate and form 3D structures. Here, we demonstrated efficient regeneration of avascular Elastic Cartilage from in vitro-grown mesenchymal condensation, which recapitulated the early stages of chondrogenesis, including transient vascularization. After transplantation of vascularized condensed progenitors into immunodeficient mice, we used an intravital imaging approach to follow Cartilage maturation. We determined that endothelial cells are present inside rudimentary Cartilage (mesenchymal condensation) prior to Cartilage maturation. Recreation of endothelial interactions in culture enabled a recently identified population of adult Elastic Cartilage progenitors to generate mesenchymal condensation in a self-driven manner, without requiring the support of exogenous inductive factors or scaffold materials. Moreover, the culture-grown 3D condensed adult-derived progenitors were amenable to storage via simple freezing methods and efficiently reconstructed 3D Elastic Cartilage upon transplantation. Together, our results indicate that transplantation of endothelialized and condensed progenitors represents a promising approach to realizing a regenerative medicine treatment for craniofacial deformations.
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Elastic Cartilage reconstruction by transplantation of cultured hyaline Cartilage-derived chondrocytes.
Transplantation Proceedings, 2014Co-Authors: M. Mizuno, Takanori Takebe, Y.h. Jo, Shinji Kobayashi, Masayuki Masutani, S. Kimura, Hideki TaniguchiAbstract:Abstract Current surgical intervention of craniofacial defects caused by injuries or abnormalities uses reconstructive materials, such as autologous Cartilage grafts. Transplantation of autologous tissues, however, places a significant invasiveness on patients, and many efforts have been made for establishing an alternative graft. Recently, we and others have shown the potential use of reconstructed Elastic Cartilage from ear-derived chondrocytes or progenitors with the unique Elastic properties. Here, we examined the differentiation potential of canine joint Cartilage–derived chondrocytes into Elastic Cartilage for expanding the cell sources, such as hyaline Cartilage. Articular chondrocytes are isolated from canine joint, cultivated, and compared regarding characteristic differences with auricular chondrocytes, including proliferation rates, gene expression, extracellular matrix production, and Cartilage reconstruction capability after transplantation. Canine articular chondrocytes proliferated less robustly than auricular chondrocytes, but there was no significant difference in the amount of sulfated glycosaminoglycan produced from redifferentiated chondrocytes. Furthermore, in vitro expanded and redifferentiated articular chondrocytes have been shown to reconstruct Elastic Cartilage on transplantation that has histologic characteristics distinct from hyaline Cartilage. Taken together, cultured hyaline Cartilage–derived chondrocytes are a possible cell source for Elastic Cartilage reconstruction.
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brief report reconstruction of joint hyaline Cartilage by autologous progenitor cells derived from ear Elastic Cartilage
Stem Cells, 2014Co-Authors: Mitsuru Mizuno, Takanori Takebe, Yuichiro Yabuki, Takahisa Matsuzaki, Lee Jeong Ik, Hiroshi Yoshikawa, Shinji Kobayashi, Seiichiro Nakabayashi, Jiro MaegawaAbstract:In healthy joints, hyaline Cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human Cartilage progenitor cells from ear Elastic Cartilage. Human Cartilage progenitor cells have high chondrogenic and proliferative potential to form Elastic Cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived Cartilage progenitor cells can be used to reconstruct hyaline Cartilage, which has different mechanical and histological properties from Elastic Cartilage. In our efforts to develop foundational technologies for joint hyaline Cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint Cartilage damage, transplanted ear-derived autologous Cartilage progenitor cells. The reconstructed Cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline Cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear Cartilage and equal to those of joint hyaline Cartilage. This study suggested that joint hyaline Cartilage was reconstructed from ear-derived Cartilage progenitor cells. It also demonstrated that ear-derived Cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline Cartilage in patients with degenerative arthropathies. Stem Cells 2014;32:816–821
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brief report reconstruction of joint hyaline Cartilage by autologous progenitor cells derived from ear Elastic Cartilage
Stem Cells, 2014Co-Authors: Mitsuru Mizuno, Takanori Takebe, Yuichiro Yabuki, Takahisa Matsuzaki, Hiroshi Yoshikawa, Shinji Kobayashi, Seiichiro Nakabayashi, Hiroomi Kan, Jiro MaegawaAbstract:In healthy joints, hyaline Cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human Cartilage progenitor cells from ear Elastic Cartilage. Human Cartilage progenitor cells have high chondrogenic and proliferative potential to form Elastic Cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived Cartilage progenitor cells can be used to reconstruct hyaline Cartilage, which has different mechanical and histological properties from Elastic Cartilage. In our efforts to develop foundational technologies for joint hyaline Cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint Cartilage damage, transplanted ear-derived autologous Cartilage progenitor cells. The reconstructed Cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline Cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear Cartilage and equal to those of joint hyaline Cartilage. This study suggested that joint hyaline Cartilage was reconstructed from ear-derived Cartilage progenitor cells. It also demonstrated that ear-derived Cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline Cartilage in patients with degenerative arthropathies.
Charles A. Vacanti - One of the best experts on this subject based on the ideXlab platform.
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The Effect of Fibroblast Growth Factor and Transforming Growth Factor-β on Porcine Chondrocytes and Tissue-Engineered Autologous Elastic Cartilage
Tissue engineering, 2001Co-Authors: Carlos A. Arévalo-silva, Yilin Cao, Martin P. Vacanti, Yulai Weng, Charles A. Vacanti, Angela Rodriguez, Roland D. EaveyAbstract:Elastic Cartilage responds mitogenically in vitro to transforming growth factor-β (TGF-β) and basic fibroblast growth factor (basic FGF). We studied the effects of these growth factors separately or in a combination on porcine auricular chondrocytes in vitro and on the autologous Elastic Cartilage produced. Cells were harvested from the Elastic auricular Cartilage of 16- to 18-kg Yorkshire swine. Viability and quantification of the cells was determined. Cells were plated at equal concentration and studied in vitro in one of four identical media environments except for the growth factors: Group I contained Ham's F-12 with supplements but no growth factors, Group II also contained basic-FGF, Group III also contained TGF-β, and Group IV also contained a combination of both growth factors. After 3 weeks in vitro, the cells were chemically dissociated with 0.25% trypsin. Cell suspensions composed of 3 × 107 cells/cc in 30% Pluronic F-127/Ham's F-12 were injected subcutaneously. Implants were harvested at 6, 8,...
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Internal Support of Tissue-Engineered Cartilage
Archives of otolaryngology--head & neck surgery, 2000Co-Authors: Carlos A. Arévalo-silva, Yilin Cao, Martin P. Vacanti, Yulai Weng, Roland D. Eavey, Charles A. VacantiAbstract:Background Auricles previously created by tissue engineering in nude mice used a biodegradable internal scaffold to maintain the desired shape of an ear. However, the biodegradable scaffold incited a compromising inflammatory response in subsequent experiments in immunocompetent animals. Objective To test the hypothesis that tissue-engineered autologous Cartilage can be bioincorporated with a nonreactive, permanent endoskeletal scaffold. Materials and Methods Auricular Elastic Cartilage was harvested from Yorkshire swine. The chondrocytes were isolated and suspended into a hydrogel (Pluronic F-127) at a cell concentration of 5 × 10 7 cells/mL. Nonbiodegradable endoskeletal scaffolds were formed with 1 of 5 polymers: (1) high-density polyethylene, (2) soft acrylic, (3) polymethylmethacrylate, (4) extrapurified Silastic, and (5) conventional Silastic. Three groups were studied: (1) a control group using only the 5 polymers, (2) the 5 polymers enveloped by Pluronic F-127 only, and (3) the implants coated with Pluronic F-127 seeded with chondrocytes. All constructs were implanted subdermally; implants containing cells were implanted into the same animal from which the cells had been islolated. The implants were harvested after 8 weeks of in vivo culture and histologically analyzed. Results Only implants coated by hydrogel plus cells generated healthy new Cartilage. With 3 polymers (high-density polyethylene, acrylic, and extrapurified Silastic), the coverage was nearly complete by Elastic Cartilage, with minimal fibroCartilage and minimal to no inflammatory reaction. The Food and Drug Administration–approved conventional Silastic implants resulted in fragments of fibrous tissue mixed with Elastic Cartilage plus evidence of chronic inflammation. The polymethylmethacrylate implant was intermediate in the amount of Cartilage formed and degree of inflammation. Conclusions This pilot technique combining tissue-engineered autologous Elastic Cartilage with a permanent biocompatible endoskeleton demonstrated success in limiting the inflammatory response to the scaffold, especially to high-density polyethylene, acrylic, and extrapurified Silastic. This model facilitates the potential to generate tissue of intricate shape, such as the human ear, by internal support.
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Influence of Growth Factors on Tissue-Engineered Pediatric Elastic Cartilage
Archives of otolaryngology--head & neck surgery, 2000Co-Authors: Carlos A. Arévalo-silva, Yilin Cao, Martin P. Vacanti, Yulai Weng, Charles A. Vacanti, Roland D. EaveyAbstract:Objective To investigate the influence of growth factors on tissue-engineered pediatric human Elastic Cartilage relative to potential clinical application. Design Controlled study. Subjects Eleven children ranging in age from 5 to 15 years provided auricular Elastic Cartilage specimens measuring approximately 1 × 1 × 0.2 cm and weighing approximately 100 mg. Interventions Three million chondrocytes were plated into 4 groups of Ham F-12 culture medium: group 1, Ham F-12 culture medium only; no growth factors (control group); group 2, Ham F-12 culture medium and basic fibroblast growth factor; group 3, Ham F-12 culture medium and transforming growth actor β; and group 4, Ham F-12 culture medium and a combination of both growth factors. At 3 weeks, the cells were harvested and mixed with a copolymer gel of polyethylene glycol and polypropylene oxide (Pluronic F-127). The cell solution was injected subcutaneously into athymic mice. The constructs were harvested at up to 22 weeks of in vivo culture and histologically analyzed. Results The average number of cells generated in vitro was as follows: group 1, 12 million; group 2, 40 million; group 3, 7 million; and group 4, 35 million. Group 2 in vivo gross specimens were the largest and heaviest. Histologically, the control group and the basic fibroblast growth factor group (groups 1 and 2) exhibited characteristics compatible with normal auricular Cartilage; groups 3 and 4 demonstrated cellular disorganization and moderate to severe fibrous tissue infiltration. Conclusions Basic fibroblast growth factor demonstrates the greatest positive influence on the in vitro and in vivo growth of engineered pediatric human auricular Cartilage. The results suggest that basic fibroblast growth factor has the potential for clinical application in which a goal will be to generate a large volume of tissue-engineered Cartilage from a small donor specimen in a short period of time and of a quality similar to native human Elastic Cartilage.
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Engineering autogenous Cartilage in the shape of a helix using an injectable hydrogel scaffold.
The Laryngoscope, 2000Co-Authors: Aminuddin B. Saim, Yilin Cao, Yulai Weng, Charles A. Vacanti, Chia‐ning Chang, Martin Vacanti, Roland D. EaveyAbstract:Objective Previous successful efforts to tissue engineer Cartilage for an auricle have used an immunocompromised nude mouse xenograft model. Subsequent efforts in an immunocompetent autogenous animal model have been less successful because of an inflammatory response directed against the foreign scaffold polymer used to provide an auricular shape. We studied an alternative polymer material and surgical technique to engineer autogenous Cartilage in the shape of a human ear helix using injectable hydrogel scaffolding, Pluronic F-127 (polyethylene oxide and polypropylene oxide). Subject Yorkshire swine. Material and Methods Fresh autogenous chondrocytes were suspended in a biodegradable, biocompatible co-polymer hydrogel, Pluronic F-127, at a concentration of 3 × 107 cells/mL. To support the contour of the implant, a skin fold channel in the shape of the helix of a human ear was created in the skin in three sites on the ventral surface of the animal. The cell-hydrogel suspension was injected through the skin fold channel. For controls, injections were made into identical channels using either cells alone or the Pluronic F-127 without cells. After 10 weeks, the specimens were excised and examined both grossly and histologically. Results Grossly, all implants retained a helical-like shape. Excised specimens possessed flexible characteristics consistent with Elastic Cartilage. The specimens could be folded and twisted and on release of mechanical pressure would instantly return to the original shape. Histological evaluation of the implants using H&E, Safranin O, trichrome blue, and Verhoeff's stains demonstrated findings consistent with mature Elastic Cartilage. Control injection of hydrogel alone demonstrated no evidence of Cartilage formation and control injection of chondrocytes alone showed evidence only of disassociated Elastic Cartilage. Conclusion Injection of autologous porcine auricular chondrocytes suspended in a biodegradable, biocompatible hydrogel of Pluronic F-127 resulted in the formation of Cartilage tissue in the approximate size and shape of a human ear helix. This preliminary method extends the concept of auricular tissue engineering from an immunocompromised xenograft animal model to an immunocompetent autologous animal model.
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Characteristics of Cartilage engineered from human pediatric auricular Cartilage.
Plastic and Reconstructive Surgery, 1999Co-Authors: Angela Rodriguez, Martin P. Vacanti, Roland D. Eavey, Clemente Ibarra, Charles A. VacantiAbstract:In the repair of Cartilage defects, autologous tissue offers the advantage of lasting biocompatibility. The ability of bovine chondrocytes isolated from hyaline Cartilage to generate tissue-engineered Cartilage in a predetermined shape, such as a human ear, has been demonstrated ; however, the potential of chondrocytes isolated from human Elastic Cartilage remains unknown. In this study, the authors examined the multiplication characteristics of human auricular chondrocytes and the ability of these cells to generate new Elastic Cartilage as a function of the length of time they are maintained in vitro. Human auricular Cartilage, harvested from patients 5 to 17 years of age, was digested in collagenase, and the chondrocytes were isolated and cultured in vitro for up to 12 weeks. Cells were trypsinized, counted, and passaged every 2 weeks. Chondrocyte-polymer (polyglycolic acid) constructs were created at each passage and then implanted into athymic mice for 8 weeks. The ability of the cells to multiply in vitro and their ability to generate new Cartilage as a function of the time they had been maintained in vitro were studied. A total of 31 experimental constructs from 12 patients were implanted and compared with a control group of constructs without chondrocytes. In parallel, a representative sample of cells was evaluated to determine the presence of collagen. The doubling rate of human auricular chondrocytes in vitro remained constant within the population studied. New tissue developed in 22 of 31 experimental implants. This tissue demonstrated the physical characteristics of auricular Cartilage on gross inspection. Histogically, specimens exhibited dense cellularity and lacunae-containing cells embedded in a basophilic matrix. The specimens resembled immature Cartilage and were partially devoid of the synthetic material of which the construct had been composed. Analyses for collagen, proteoglycans, and elastin were consistent with Elastic Cartilage. No Cartilage was detected in the control implants. Human auricular chondrocytes multiply well in vitro and possess the ability to form new Cartilage when seeded onto a three-dimensional scaffold. These growth characteristics might some day enable chondrocytes isolated from a small auricular biopsy to be expanded in vitro to generate a large, custom-shaped, autologous graft for clinical reconstruction of a Cartilage defect, such as for congenital microtia.
Yuichiro Yabuki - One of the best experts on this subject based on the ideXlab platform.
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Development of a Method for Scaffold-Free Elastic Cartilage Creation.
International journal of molecular sciences, 2020Co-Authors: Masahiro Enomura, Yuichiro Yabuki, Shinji Kobayashi, Soichiro Murata, Yuri Terado, Maiko Tanaka, Takayoshi Oba, Shintaro Kagimoto, Kenichi Morita, Toshimasa UemuraAbstract:Microtia is a congenital aplasia of the auricular Cartilage. Conventionally, autologous costal Cartilage grafts are collected and shaped for transplantation. However, in this method, excessive invasion occurs due to limitations in the costal Cartilage collection. Due to deformation over time after transplantation of the shaped graft, problems with long-term morphological maintenance exist. Additionally, the lack of Elasticity with costal Cartilage grafts is worth mentioning, as costal Cartilage is a type of hyaline Cartilage. Medical plastic materials have been transplanted as alternatives to costal Cartilage, but transplant rejection and deformation over time are inevitable. It is imperative to create tissues for transplantation using cells of biological origin. Hence, Cartilage tissues were developed using a biodegradable scaffold material. However, such materials suffer from transplant rejection and biodegradation, causing the transplanted Cartilage tissue to deform due to a lack of Elasticity. To address this problem, we established a method for creating Elastic Cartilage tissue for transplantation with autologous cells without using scaffold materials. Chondrocyte progenitor cells were collected from perichondrial tissue of the ear Cartilage. By using a multilayer culture and a three-dimensional rotating suspension culture vessel system, we succeeded in creating scaffold-free Elastic Cartilage from Cartilage progenitor cells.
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autotransplantation of monkey ear perichondrium derived progenitor cells for Cartilage reconstruction
Cell Transplantation, 2016Co-Authors: Shintaro Kagimoto, Takanori Takebe, Yuichiro Yabuki, Shinji Kobayashi, Toshimasa Uemura, Ayaka Hori, Koichi Hirotomi, Taro Mikami, Jiro MaegawaAbstract:We recently developed a promising regenerative method based on the xenotransplantation of human Cartilage progenitor cells, demonstrating self-renewing Elastic Cartilage reconstruction with expected long-term tissue restoration. However, it remains unclear whether autotransplantation of Cartilage progenitors may work by a similar principle in immunocompetent individuals. We used a nonhuman primate (monkey) model to assess the safety and efficacy of our regenerative approach because the model shares characteristics with humans in terms of biological functions, including anatomical features. First, we identified the expandable and multipotent progenitor population from monkey ear perichondrium and succeeded in inducing chondrocyte differentiation in vitro. Second, in vivo transplanted progenitor cells were capable of reconstructing Elastic Cartilage by xenotransplantation into an immunodeficient mouse. Finally, the autologous monkey progenitor cells were transplanted into the subcutaneous region of a craniofacial section and developed mature Elastic Cartilage of their own 3 months after transplantation. Furthermore, we attempted to develop a clinically relevant, noninvasive monitoring method using magnetic resonance imaging (MRI). Collectively, this report shows that the autologous transplantation of Cartilage progenitors is potentially effective for reconstructing Elastic Cartilage. This principle will be invaluable for repairing craniofacial injuries and abnormalities in the context of plastic and reconstructive surgery.
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brief report reconstruction of joint hyaline Cartilage by autologous progenitor cells derived from ear Elastic Cartilage
Stem Cells, 2014Co-Authors: Mitsuru Mizuno, Takanori Takebe, Yuichiro Yabuki, Takahisa Matsuzaki, Lee Jeong Ik, Hiroshi Yoshikawa, Shinji Kobayashi, Seiichiro Nakabayashi, Jiro MaegawaAbstract:In healthy joints, hyaline Cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human Cartilage progenitor cells from ear Elastic Cartilage. Human Cartilage progenitor cells have high chondrogenic and proliferative potential to form Elastic Cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived Cartilage progenitor cells can be used to reconstruct hyaline Cartilage, which has different mechanical and histological properties from Elastic Cartilage. In our efforts to develop foundational technologies for joint hyaline Cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint Cartilage damage, transplanted ear-derived autologous Cartilage progenitor cells. The reconstructed Cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline Cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear Cartilage and equal to those of joint hyaline Cartilage. This study suggested that joint hyaline Cartilage was reconstructed from ear-derived Cartilage progenitor cells. It also demonstrated that ear-derived Cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline Cartilage in patients with degenerative arthropathies. Stem Cells 2014;32:816–821
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brief report reconstruction of joint hyaline Cartilage by autologous progenitor cells derived from ear Elastic Cartilage
Stem Cells, 2014Co-Authors: Mitsuru Mizuno, Takanori Takebe, Yuichiro Yabuki, Takahisa Matsuzaki, Hiroshi Yoshikawa, Shinji Kobayashi, Seiichiro Nakabayashi, Hiroomi Kan, Jiro MaegawaAbstract:In healthy joints, hyaline Cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human Cartilage progenitor cells from ear Elastic Cartilage. Human Cartilage progenitor cells have high chondrogenic and proliferative potential to form Elastic Cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived Cartilage progenitor cells can be used to reconstruct hyaline Cartilage, which has different mechanical and histological properties from Elastic Cartilage. In our efforts to develop foundational technologies for joint hyaline Cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint Cartilage damage, transplanted ear-derived autologous Cartilage progenitor cells. The reconstructed Cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline Cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear Cartilage and equal to those of joint hyaline Cartilage. This study suggested that joint hyaline Cartilage was reconstructed from ear-derived Cartilage progenitor cells. It also demonstrated that ear-derived Cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline Cartilage in patients with degenerative arthropathies.
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Human Elastic Cartilage engineering from Cartilage progenitor cells using rotating wall vessel bioreactor.
Transplantation Proceedings, 2012Co-Authors: Takanori Takebe, Takuro Adegawa, Yuichiro Yabuki, J. Tanaka, Shinji Kobayashi, Tomohiko Yoshioka, M. Mizuno, H. Suzuki, Jiro MaegawaAbstract:Abstract Transplantation of bioengineered Elastic Cartilage is considered to be a promising approach for patients with craniofacial defects. We have previously shown that human ear perichondrium harbors a population of Cartilage progenitor cells (CPCs). The aim of this study was to examine the use of a rotating wall vessel (RWV) bioreactor for CPCs to engineer 3-D Elastic Cartilage in vitro. Human CPCs isolated from ear perichondrium were expanded and differentiated into chondrocytes under 2-D culture conditions. Fully differentiated CPCs were seeded into recently developed pC-HAp/ChS (porous material consisted of collagen, hydroxyapatite, and chondroitinsulfate) scaffolds and 3-D cultivated utilizing a RWV bioreactor. 3-D engineered constructs appeared shiny with a yellowish, Cartilage-like morphology. The shape of the molded scaffold was maintained after RWV cultivation. Hematoxylin and eosin staining showed engraftment of CPCs inside pC-HAp/ChS. Alcian blue and Elastica Van Gieson staining showed of proteoglycan and Elastic fibers, which are unique extracellular matrices of Elastic Cartilage. Thus, human CPCs formed Elastic Cartilage-like tissue after 3-D cultivation in a RWV bioreactor. These techniques may assist future efforts to reconstruct complicate structures composed of Elastic Cartilage in vitro.