The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Eichi Tsuruga - One of the best experts on this subject based on the ideXlab platform.
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Intracellular interaction of EMILIN-1 with fibrillin-1 in human periodontal ligament cells
Orthodontic Waves, 2012Co-Authors: Yuka Nakatomi, Yoshinori Yamauchi, Kaori Yamanouchi, Kazuki Nakashima, Yoshihiko Sawa, Megumi Kawagoe, Eichi Tsuruga, Hiroyuki IshikawaAbstract:Abstract The elastic system fibers comprise oxytalan, Elaunin and elastic fibers, differing in their relative microfibril and elastin contents. Human periodontal ligaments (PDLs) contain oxytalan fibers (pure microfibrils), which are composed mainly of fibrillin-1, the major component of microfibrils. We recently demonstrated that EMILIN-1, located at the interface between elastin and microfibrils, controls the amount of fibrillin-1 assembly in PDL fibroblast cell/matrix layers [8] , although the mechanism involved was unclear. We subjected cultured PDL fibroblasts to immunofluorescence and immunoprecipitation assays in order to examine the intracellular interaction of EMILIN-1 with fibrillin-1. Immunofluorescence showed that EMILIN-1 was colocalized with fibrillin-1, both inside and outside the cells. Additionally, EMILIN-1 formed a complex with fibrillin-1 in the intracellular fraction. These results suggest that EMILIN-1 may form complexes with fibrillin-1 in cellular vesicles, thus contributing effectively to the initial assembly of pericellular fibrillin-1 during the process of oxytalan fiber formation.
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Intracellular interaction of EMILIN-1 with fibrillin-1 in human periodontal ligament cells
Orthodontic Waves, 2012Co-Authors: Yuka Nakatomi, Yoshinori Yamauchi, Kaori Yamanouchi, Kazuki Nakashima, Yoshihiko Sawa, Megumi Kawagoe, Eichi Tsuruga, Hiroyuki IshikawaAbstract:The elastic system fibers comprise oxytalan, Elaunin and elastic fibers, differing in their relative microfibril and elastin contents. Human periodontal ligaments (PDLs) contain oxytalan fibers (pu...
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EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro
Connective tissue research, 2010Co-Authors: Yuka Nakatomi, Kazuki Nakashima, Yoshihiko Sawa, Eichi Tsuruga, Hiroyuki IshikawaAbstract:The elastic system fibers comprise oxytalan, Elaunin, and elastic fibers, differing in their relative microfibril and elastin contents. Among them, human periodontal ligament (PDL) contains only oxytalan fibers (pure microfibrils). Elastin microfibril interface-located protein-1 (EMILIN-1) is localized at the interface between microfibrils and elastin. We hypothesized that EMILIN-1 may contribute to the formation of oxytalan fibers. We used a small interfering RNA (siRNA) for EMILIN-1 in PDL cell culture to examine the extracellular deposition of fibrillin-1 (the major component of microfibrils). EMILIN-1 was labeled on microfibrils positive for fibrillin-1 and was colocalized with fibrillin-1 upon immunoprecipitation assay. EMILIN-1 suppression reduced the level of fibrillin-1 deposition to 23% of the control, and this was responsible for the diminution of fibrillin-1 deposition revealed by immunofluorescence. These results suggest that EMILIN-1 may regulate the formation of oxytalan fibers and play a ro...
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Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells.
Acta histochemica et cytochemica, 2009Co-Authors: Yutaka Hisanaga, Yuka Nakatomi, Kazuki Nakashima, Eichi Tsuruga, Hiroyuki Ishikawa, Yuji Hatakeyama, Yoshihiko SawaAbstract:The elastic system fibers comprise oxytalan, Elaunin and elastic fibers, which differ in their relative microfibril and elastin content. Human periodontal ligaments (PDL) contain only oxytalan fibers (pure microfibrils) among them. Since fibulin-5 regulates the organization of elastic fibers to link the fibers to cells, we hypothesized that fibulin-5 may contribute to the formation of oxytalan fibers. We used siRNA for fibulin-5 in PDL cell culture to examine the extracellular deposition of fibrillin-1 and -2, which are the major components of microfibrils. Fibulin-5 was labeled on microfibrils positive for fibrillin-1 and -2. Fibulin-5 suppression reduced the level of fibrillin-1 and -2 deposition to 60% of the control level. These results suggest that fibulin-5 may control the formation of oxytalan fibers, and play a role in the homeostasis of oxytalan fibers.
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Regular Histochemica Society Japan Articleof Histochemistry et Cytochemica and Cytochemistry Fibulin-5 Contributes to Microfibril Assembly in Human Periodontal Ligament Cells
2009Co-Authors: Yutaka Hisanaga, Yuka Nakatomi, Kazuki Nakashima, Eichi Tsuruga, Hiroyuki Ishikawa, Yuji Hatakeyama, Yoshihiko SawaAbstract:© 2009 The The elastic Japan system Society of fibers Histochemistry comprise oxytalan, and Cy- Elaunin and elastic fibers, which differ in their relative microfibril and elastin content. Human periodontal ligaments (PDL) contain only oxytalan fibers (pure microfibrils) among them. Since fibulin-5 regulates the organization of elastic fibers to link the fibers to cells, we hypothesized that fibulin-5 may contribute to the formation of oxytalan fibers. We used siRNA for fibulin-5 in PDL cell culture to examine the extracellular deposition of fibrillin-1 and-2, which are the major components of microfibrils. Fibulin-5 was labeled on microfibrils positive for fibrillin-1 and-2. Fibulin-5 suppression reduced the level of fibrillin-1 and-2 deposition to 60 % of the control level. These results suggest that fibulin-5 may control the formation of oxytalan fibers, and play a role in the homeostasis of oxytalan fibers. Key words: fibrillin, fibulin-5, periodontal ligaments, oxytalan fiber, microfibrils I
Toshihiko Yajima - One of the best experts on this subject based on the ideXlab platform.
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gene expression and accumulation of fibrillin 1 fibrillin 2 and tropoelastin in cultured periodontal fibroblasts
Journal of Dental Research, 2002Co-Authors: Eichi Tsuruga, Kazuharu Irie, Toshihiko YajimaAbstract:The elastic system fibers consist of three types—oxytalan, Elaunin, and elastic fibers—differing in their relative microfibril and elastin contents. All three types are found in human gingiva, but human periodontal ligaments contain only elastin-free fibers. We examined cultured human gingival fibroblasts (HGF) and cultured human periodontal ligament fibroblasts (HPLF) to determine the gene expression of fibrillin-1 and fibrillin-2 (the major components of microfibrils) and of tropoelastin. In addition, we assessed the degree of accumulation of these proteins in the extracellular matrix. Northern blot analysis revealed that the level of expression of fibrillin-1 and fibrillin-2 was higher in HGF than in HPLF. However, examination of matrix samples from HGF and HPLF cell layers showed that there was no difference in fibrillin-1 accumulation, although fibrillin-2 accumulated to a much greater extent in the HGF-derived matrix. Tropoelastin was expressed only in and around HGF. These results show a correlatio...
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Tropoelastin Expression by Periodontal Fibroblasts
Journal of dental research, 2002Co-Authors: Eichi Tsuruga, Kazuharu Irie, Yasunori Sakakura, Toshihiko YajimaAbstract:Elastic system fibers are load-bearing proteins found in periodontal tissue. There are three types—oxytalan, Elaunin, and elastic fibers—which differ in their relative microfibril and elastin contents. Oxytalan fibers are known to be distributed in the periodontal ligaments and gingiva, whereas Elaunin and elastic fibers are present only in the gingiva. We examined gene expression and accumulation of tropoelastin in the cell-matrix layers of human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF) in vitro. HGF and HPLF were cultured in MEM containing 10% newborn calf serum for 8 wks. Northern blotting and RT-PCR analyses showed that only HGF expressed mRNA encoding tropoelastin. Western blotting analysis demonstrated 77-kDa protropoelastin and 68-kDa tropoelastin only in the cell-matrix layer of HGF cultured for 8 wks. These results suggest that the different tropoelastin expression patterns reflect the difference between HGF and HPLF phenotypes.
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Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro.
Journal of periodontal research, 2002Co-Authors: Eichi Tsuruga, Kazuharu Irie, Yasunori Sakakura, Toshihiko YajimaAbstract:The elastic system fibers consist of three different types, oxytalan, Elaunin and elastic fibers, which differ in the relative content of microfibrils and elastin. In periodontal tissues, oxytalan fibers are known to be distributed in the periodontal ligament and gingiva, while Elaunin and elastic fibers are present only in the gingiva. We examined the in vitro synthesis of microfibrils and elastin by human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF). The two kinds of HGF and HPLF were cultured in MEM containing 10% newborn calf serum for 30 days. Since fibrillin-1 and fibrillin-2 are the major components of microfibrils involved in elastogenesis, we investigated the synthesis of fibrillins and tropoelastin in the conditioned medium of HGF and HPLF. Western blot analysis revealed fibrillin-1 and fibrillin-2 to occur in the HGF and HPLF culture medium, HGF exhibiting a higher level of synthesis than HPLF. Tropoelastin, on the other hand, was detected only in the medium of HGF after day 24. In addition, analysis of RNA extracted from HGF and HPLF on day 30 showed that only HGF expressed mRNA encoding tropoelastin. Immunohistochemically, accumulation of tropoelastin in the perinuclear area was found only in HGF. These results show that HGF expressed microfibrils and elastin, while HPLF expressed only microfibrils for the experimental period, and suggest a biochemical basis for the different distribution of elastic system fibers of the gingiva and periodontal ligament in vivo.
Kazuharu Irie - One of the best experts on this subject based on the ideXlab platform.
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gene expression and accumulation of fibrillin 1 fibrillin 2 and tropoelastin in cultured periodontal fibroblasts
Journal of Dental Research, 2002Co-Authors: Eichi Tsuruga, Kazuharu Irie, Toshihiko YajimaAbstract:The elastic system fibers consist of three types—oxytalan, Elaunin, and elastic fibers—differing in their relative microfibril and elastin contents. All three types are found in human gingiva, but human periodontal ligaments contain only elastin-free fibers. We examined cultured human gingival fibroblasts (HGF) and cultured human periodontal ligament fibroblasts (HPLF) to determine the gene expression of fibrillin-1 and fibrillin-2 (the major components of microfibrils) and of tropoelastin. In addition, we assessed the degree of accumulation of these proteins in the extracellular matrix. Northern blot analysis revealed that the level of expression of fibrillin-1 and fibrillin-2 was higher in HGF than in HPLF. However, examination of matrix samples from HGF and HPLF cell layers showed that there was no difference in fibrillin-1 accumulation, although fibrillin-2 accumulated to a much greater extent in the HGF-derived matrix. Tropoelastin was expressed only in and around HGF. These results show a correlatio...
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Tropoelastin Expression by Periodontal Fibroblasts
Journal of dental research, 2002Co-Authors: Eichi Tsuruga, Kazuharu Irie, Yasunori Sakakura, Toshihiko YajimaAbstract:Elastic system fibers are load-bearing proteins found in periodontal tissue. There are three types—oxytalan, Elaunin, and elastic fibers—which differ in their relative microfibril and elastin contents. Oxytalan fibers are known to be distributed in the periodontal ligaments and gingiva, whereas Elaunin and elastic fibers are present only in the gingiva. We examined gene expression and accumulation of tropoelastin in the cell-matrix layers of human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF) in vitro. HGF and HPLF were cultured in MEM containing 10% newborn calf serum for 8 wks. Northern blotting and RT-PCR analyses showed that only HGF expressed mRNA encoding tropoelastin. Western blotting analysis demonstrated 77-kDa protropoelastin and 68-kDa tropoelastin only in the cell-matrix layer of HGF cultured for 8 wks. These results suggest that the different tropoelastin expression patterns reflect the difference between HGF and HPLF phenotypes.
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Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro.
Journal of periodontal research, 2002Co-Authors: Eichi Tsuruga, Kazuharu Irie, Yasunori Sakakura, Toshihiko YajimaAbstract:The elastic system fibers consist of three different types, oxytalan, Elaunin and elastic fibers, which differ in the relative content of microfibrils and elastin. In periodontal tissues, oxytalan fibers are known to be distributed in the periodontal ligament and gingiva, while Elaunin and elastic fibers are present only in the gingiva. We examined the in vitro synthesis of microfibrils and elastin by human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF). The two kinds of HGF and HPLF were cultured in MEM containing 10% newborn calf serum for 30 days. Since fibrillin-1 and fibrillin-2 are the major components of microfibrils involved in elastogenesis, we investigated the synthesis of fibrillins and tropoelastin in the conditioned medium of HGF and HPLF. Western blot analysis revealed fibrillin-1 and fibrillin-2 to occur in the HGF and HPLF culture medium, HGF exhibiting a higher level of synthesis than HPLF. Tropoelastin, on the other hand, was detected only in the medium of HGF after day 24. In addition, analysis of RNA extracted from HGF and HPLF on day 30 showed that only HGF expressed mRNA encoding tropoelastin. Immunohistochemically, accumulation of tropoelastin in the perinuclear area was found only in HGF. These results show that HGF expressed microfibrils and elastin, while HPLF expressed only microfibrils for the experimental period, and suggest a biochemical basis for the different distribution of elastic system fibers of the gingiva and periodontal ligament in vivo.
S. P. Pylypas - One of the best experts on this subject based on the ideXlab platform.
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A re-evaluation of the distribution of the elastic meshwork within the periodontal ligament of the mouse.
Journal of Periodontal Research, 1992Co-Authors: Roger B. Johnson, S. P. PylypasAbstract:The elastic properties of the periodontal ligament have been attributed, in part, to oxytalan fibers, as no other types of elastic fibers are described there. It has been difficult to study the periodontal elastic meshwork by standard microscopic techniques because it is partially obscured by the adjacent periodontal ligament collagen fibers. Our study employed methods which either completely or partially removed mandibular molar periodontal ligament collagen fibers, exposing a previously undescribed periodontal elastic meshwork. The periodontal elastic meshwork was composed of many elastin lamellae containing both peripheral microfibrils of regular arrangement and central microfibrils of irregular arrangement, which could only be demonstrated in oxidized tissues. Peripheral, regularly arranged bundles of microfibrils resembled oxytalan fibers, which were often adherent to the border of the elastin lamella. Elastin lamellae containing irregular microfibrils resembled Elaunin fibers. These fibers probably enclosed either blood vessels, nerves or collagen fiber bundles. Peripheral microfibrils attached Elaunin to cementum, alveolar bone, blood vessels, and principal periodontal collagen fibers. Thus, the periodontal elastic meshwork is composed of both oxytalan and Elaunin fibers. Microfibrils attach Elaunin fibers to the adjacent non-elastic tissue and also form bundles which traverse the periodontal ligament space and are probably the oxytalan fibers demonstrable by light microscopic techniques. This meshwork of oxytalan and Elaunin fibers probably contributes to tooth support and maintenance of periodontal homeostasis by dissipating chewing forces and maintaining patency of periodontal blood vessels.
Radovan Borojevic - One of the best experts on this subject based on the ideXlab platform.
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Photoaged Skin Therapy with Adipose-Derived Stem Cells.
Plastic and reconstructive surgery, 2020Co-Authors: Luiz Charles-de-sá, Natale Ferreira Gontijo-de-amorim, Gino Rigotti, Andrea Sbarbati, Paolo Bernardi, Donatella Benati, Rosana Bizon Vieira Carias, Christina Maeda Takiya, Radovan BorojevicAbstract:Background The major intrinsic cause of facial skin degeneration is age, associated with extrinsic factors such as exposure to sun. Its major pathologic causes are degeneration of the elastin matrix, with loss of oxytalan and Elaunin fibers in the subepidermal region, and actinic degeneration of elastin fibers that lose their functional properties in the deep dermis. Therapy using autologous adipose mesenchymal stem cells for regeneration of extracellular matrix in patients with solar elastosis was addressed in qualitative and quantitative analyses of the dermal elastic fiber system and the associated cells. Methods Mesenchymal stem cells were obtained from lipoaspirates, expanded in vitro, and introduced into the facial skin of patients submitted after 3 to 4 months to a face-lift operation. In the retrieved skin, immunocytochemical analyses quantified elastic matrix components; cathepsin K; matrix metalloproteinase 12 (macrophage metalloelastase); and the macrophage M2 markers CD68, CD206, and hemeoxygenase-1. Results A full de novo formation of oxytalan and Elaunin fibers was observed in the subepidermal region, with reconstitution of the papillary structure of the dermal-epidermal junction. Elastotic deposits in the deep dermis were substituted by a normal elastin fiber network. The coordinated removal of the pathologic deposits and their substitution by the normal ones was concomitant with activation of cathepsin K and matrix metalloproteinase 12, and with expansion of the M2 macrophage infiltration. Conclusion The full regeneration of solar elastosis was obtained by injection of in vitro expanded autologous adipose mesenchymal stem cells, which are appropriate, competent, and sufficient to elicit the full structural regeneration of the sun-aged skin. Clinical question/level of evidence Therapeutic, IV.