The Experts below are selected from a list of 39570 Experts worldwide ranked by ideXlab platform
David L Kaplan - One of the best experts on this subject based on the ideXlab platform.
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method to form a fiber growth factor dual gradient along electrospun silk for nerve regeneration
ACS Applied Materials & Interfaces, 2014Co-Authors: Tony Dinis, David L Kaplan, Roberto Elia, Guillaume Vidal, Adrien Auffret, Christophe EglesAbstract:Concentration gradients of guidance molecules influence cell behavior and growth in biological tissues and are therefore of interest for the design of biomedical scaffolds for regenerative medicine. We developed an electrospining method to generate a dual-gradient of bioactive molecules and fiber density along electrospun nanofibers without any post spinning treatment. Functionalization with fluorescent molecules demonstrated the efficiency of the method to generate a discontinuous concentration gradient along the aligned fibers. As a proof of concept for tissue engineering, the silk nanofibers were functionalized with increasing concentrations of nerve growth factor (NGF) and the biological activity was assessed and quantified with rat dorsal root ganglion (DRG) neurons cultures. Protein assays showed the absence of passive release of NGF from the functionalized fibers. The results demonstrated that the NGF concentration gradient led to an oriented and increased growth of DRG neurons (417.6 ± 55.7 μm) compared to a single uniform NGF concentration (264.5 ± 37.6 μm). The easy-to-use Electrospinning technique combined with the multiple molecules that can be used for fiber functionalization makes this technique versatile for a broad range of applications from biosensors to regenerative medicine.
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Hyoungjoon Jin, Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900 000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabr...
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900 000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabr...
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Hyoungjoon Jin, Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900,000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabrication of biomaterial scaffolds based on this unique fibrous protein.
Sergey V Fridrikh - One of the best experts on this subject based on the ideXlab platform.
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Hyoungjoon Jin, Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900 000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabr...
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900 000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabr...
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Hyoungjoon Jin, Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900,000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabrication of biomaterial scaffolds based on this unique fibrous protein.
Andreas Greiner - One of the best experts on this subject based on the ideXlab platform.
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Electrospinning of manmade and biopolymer nanofibers progress in techniques materials and applications
Advanced Functional Materials, 2009Co-Authors: Seema Agarwal, Andreas Greiner, Joachin H WendorffAbstract:Electrospinning of nanofibers has developed quickly from a laboratory curiosity to a highly versatile method for the preparation of a wide variety of nanofibers, which are of interest from a fundamental as well as a technical point of view. A wide variety of materials has been processed into individual nanofibers or nanofiber mats with very different morphologies. The diverse properties of these nanofibers, based on different physical, chemical, or biological behavior, mean they are of interest for different applications ranging from filtration, antibacterial coatings, drug release formulations, tissue engineering, living membranes, sensors, and so on. A particular advantage of Electrospinning is that numerous non-fiber forming materials can be immobilized by Electrospinning in nanofiber nonwovens, even very sensitive biological objects such as virus, bacteria, and cells. The progress made during the last few years in the field of Electrospinning is fascinating and is highlighted in this Feature Article, with particular emphasis on results obtained in the authors' research units. Specific areas of importance for the future of Electrospinning, and which may open up novel applications, are also highlighted.
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Progress in the field of Electrospinning for tissue engineering applications
Advanced Materials, 2009Co-Authors: Seema Agarwal, Joachim H. Wendorff, Andreas GreinerAbstract:Electrospinning is an extremely promising method for the preparation of tissue engineering (TE) scaffolds. This technique provides nonwovens resembling in their fibrillar structures those of the extracellular matrix (ECM), and offering large surface areas, ease of functionalization for various purposes, and controllable mechanical properties. The recent developments toward large-scale productions combined with the simplicity of the process render this technique very attractive. Progress concerning the use of Electrospinning for TE applications has advanced impressively. Different groups have tackled the problem of Electrospinning for TE applications from different angles. Nowadays, Electrospinning of the majority of biodegradable and biocompatible polymers, either synthetic or natural, for TE applications is straightforward. Different issues, such as cell penetration, incorporation of growth and differentiating factors, toxicity of solvents used, productivity, functional gradient, etc. are main points of current considerations. The progress in the use of Electrospinning for TE applications is highlighted in this article with focus on major problems encountered and on various solutions available until now.
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use of Electrospinning technique for biomedical applications
Polymer, 2008Co-Authors: Seema Agarwal, Joachim H. Wendorff, Andreas GreinerAbstract:The Electrospinning technique provides non-wovens to the order of few nanometers with large surface areas, ease of functionalisation for various purposes and superior mechanical properties. Also, the possibility of large scale productions combined with the simplicity of the process makes this technique very attractive for many different applications. Biomedical field is one of the important application areas among others utilising the technique of Electrospinning like filtration and protective material, electrical and optical applications, sensors, nanofiber reinforced composites etc. Electrospinning assembly can be modified in different ways for combining materials properties with different morphological structures for these applications. The importance of Electrospinning, in general, for biomedical applications like tissue engineering drug release, wound dressing, enzyme immobilization etc. is highlighted in this feature article. The focus is also on the types of materials that have been electrospun and the modifications that have been carried out in conventional Electrospinning apparatus keeping in view the specific needs for various biomedical applications.
M Wang - One of the best experts on this subject based on the ideXlab platform.
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a novel technique for the fabrication of 3d nanofibrous scaffolds using simultaneous positive voltage Electrospinning and negative voltage Electrospinning
Materials Letters, 2013Co-Authors: Howang Tong, M WangAbstract:Abstract Electrospinning is a very attractive technology for fabricating nanofibrous structures. However, for potential tissue engineering applications, the conventional Electrospinning technique can only produce 2D nanofibrous membranes with limited thickness. In this investigation, a novel technique, which involved simultaneous positive voltage Electrospinning (PVES) and negative voltage Electrospinning (NVES), was developed for constructing 3D nanofibrous scaffolds with greatly increased thickness. Using a specially designed Electrospinning device, the problem of attraction and agglomeration of oppositely charged fibers in mid-air during simultaneous PVES and NVES could be avoided. As a demonstration and for comparison, two types of polymers, polyvinyl alcohol and poly( d,l -lactic acid), were processed into fibrous scaffolds using conventional and novel Electrospinning techniques, respectively. For each polymer, the novel technique rendered the formation of 3D fibrous scaffolds that could not be achieved via conventional Electrospinning. The polymer type, applied voltage and scaffold fabrication technique were critical factors affecting the thickness of nanofibrous scaffolds. The mechanism for 3D scaffold formation was proposed.
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a new nanofiber fabrication technique based on coaxial Electrospinning
Materials Letters, 2012Co-Authors: Howang Tong, Xin Zhang, M WangAbstract:Abstract In tissue engineering, nanofibrous scaffolds can achieve better biological responses than microfibrous scaffolds and Electrospinning is a common method for producing fibrous scaffolds. However, not all biopolymers can be made into nanofibers through conventional Electrospinning. The current investigation developed an innovative nanofiber fabrication technique based on coaxial Electrospinning and used poly(hydroxybutyrate -co- hydroxyvalerate) (PHBV) as an example for achieving nanofibers. For obtaining PHBV nanofibers, core–shell structured fibers were fabricated first via coaxial Electrospinning, with PHBV being the core and chitosan being the shell. The chitosan shell was then removed by washing electrospun scaffolds with water, leading to the formation of nanofibrous PHBV scaffolds. The PHBV nanofiber diameter was affected by the inner polymer (i.e., PHBV) solution concentration during coaxial Electrospinning, which can be explained in terms of the coaxial Electrospinning process and polymer solution viscosity. Compared to the approach of using a conductivity-enhancing salt in polymer solution to produce polymer nanofibers, the new technique not only eliminates the biocompatibility concerns but also provides a more effective way of reducing fiber diameters to the nano-size range.
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conventional Electrospinning vs emulsion Electrospinning a comparative study on the development of nanofibrous drug biomolecule delivery vehicles
Advanced Materials Research, 2011Co-Authors: Chong Wang, Sze Nga Tong, Yuk Hang Tse, M WangAbstract:Over the past decade, intensive research has been conducted on Electrospinning of fibrous tissue engineering scaffolds and their applications in body tissue regeneration. For providing multifunctions and/or enhancing the biological performance, drugs or biomolecules can be incorporated in electrospun fibers using normally one of these techniques: (1) direct dissolution, (3) emulsion Electrospinning, and (3) coaxial Electrospinning. In this investigation, for constructing nanofibrous delivery vehicles, conventional Electrospinning using polymer solutions with directly dissolved drugs or biomolecules and emulsion Electrospinning were studied and compared. Bovine serum albumin (BSA) was used as a model protein and the drug was rifamycin, a hydrophobic antibiotic. A poly (lactic-co-glycolic acid) containing the protein or drug was electrospun into fibers. In these two routes of fabricating drug-or biomolecule-loaded nanofibers, different polymer concentrations and emulsion formulations were investigated. Various aspects of the fibrous delivery vehicles were investigated using several techniques and the in vitro release behaviour was studied.
Gregory C Rutledge - One of the best experts on this subject based on the ideXlab platform.
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Hyoungjoon Jin, Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900 000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabr...
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900 000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabr...
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Electrospinning bombyx mori silk with poly ethylene oxide
Biomacromolecules, 2002Co-Authors: Hyoungjoon Jin, Sergey V Fridrikh, Gregory C Rutledge, David L KaplanAbstract:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore Electrospinning for protein-based biomaterials to fabricate scaffolds and membranes from regenerated silkworm silk, Bombyx mori, solutions. To improve processability of the protein solution, poly(ethylene oxide) (PEO) with molecular weight of 900,000 was blended with the silk fibroin. A variety of compositions of the silk/PEO aqueous blends were successfully electrospun. The morphology of the fibers was characterized using high-resolution scanning electron microscopy. Fiber diameters were uniform and less than 800 nm. The composition was estimated by X-ray photoelectron spectroscopy to characterize silk/PEO surface content. Aqueous-based electrospining of silk and silk/PEO blends provides potentially useful options for the fabrication of biomaterial scaffolds based on this unique fibrous protein.