The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Michael K. Skinner - One of the best experts on this subject based on the ideXlab platform.
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Endocrine Disruptors in 2015: Epigenetic transgenerational inheritance.
Nature reviews. Endocrinology, 2015Co-Authors: Michael K. SkinnerAbstract:Endocrine Disruptors are critical environmental exposures that influence health and can promote epigenetic transgenerational inheritance of disease and abnormal physiology. Advances in 2015 included analyses of the effects of Endocrine Disruptors on human disease, further examples of Endocrine Disruptors promoting transgenerational behavioural effects, insights into effects of Endocrine Disruptors on epigenetic programming of primordial germ cells and the finding that Endocrine Disruptors can transgenerationally promote genetic mutations.
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epigenetic transgenerational actions of Endocrine Disruptors
Reproductive Toxicology, 2011Co-Authors: Michael K. Skinner, Mohan Manikkam, Carlos GuerrerobosagnaAbstract:Environmental factors have a significant impact on biology. Therefore, environmental toxicants through similar mechanisms can modulate biological systems to influence physiology and promote disease states. The majority of environmental toxicants do not have the capacity to modulate DNA sequence, but can alter the epigenome. In the event an environmental toxicant such as an Endocrine disruptor modifies the epigenome of a somatic cell, this may promote disease in the individual exposed, but not be transmitted to the next generation. In the event a toxicant modifies the epigenome of the germ line permanently, then the disease promoted can become transgenerationaly transmitted to subsequent progeny. The current review focuses on the ability of environmental factors such as Endocrine Disruptors to promote transgenerational phenotypes.
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epigenetic transgenerational actions of Endocrine Disruptors
Endocrinology, 2006Co-Authors: Matthew D Anway, Michael K. SkinnerAbstract:Endocrine Disruptors have recently been shown to promote an epigenetic transgenerational phenotype involving a number of disease states (e.g. male infertility). The anti-androgenic fungicide vinclozolin was found to act transiently at the time of embryonic sex determination to promote in the F1 generation a spermatogenic cell defect and subfertility in the male. When the animals were allowed to age up to 1 yr, a number of other disease states developed. This phenotype was transferred through the male germ line to all subsequent generations analyzed (F1-F4). The ability of an environmental factor (i.e. Endocrine disruptor) to promote an epigenetic transgenerational phenotype impacts the potential hazards of environmental toxins, mechanisms of disease etiology, and evolutionary biology. The biological importance of the epigenetic actions of environmental agents is reviewed in the context of the primordial germ cell and development of epigenetic transgenerational phenotypes.
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epigenetic transgenerational actions of Endocrine Disruptors and male fertility
Science, 2005Co-Authors: Matthew D Anway, Mehmet Uzumcu, Andrea S Cupp, Michael K. SkinnerAbstract:Transgenerational effects of environmental toxins require either a chromosomal or epigenetic alteration in the germ line. Transient exposure of a gestating female rat during the period of gonadal sex determination to the Endocrine Disruptors vinclozolin (an antiandrogenic compound) or methoxychlor (an estrogenic compound) induced an adult phenotype in the F1 generation of decreased spermatogenic capacity (cell number and viability) and increased incidence of male infertility. These effects were transferred through the male germ line to nearly all males of all subsequent generations examined (that is, F1 to F4). The effects on reproduction correlate with altered DNA methylation patterns in the germ line. The ability of an environmental factor (for example, Endocrine disruptor) to reprogram the germ line and to promote a transgenerational disease state has significant implications for evolutionary biology and disease etiology.
Michele Prevost - One of the best experts on this subject based on the ideXlab platform.
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ozone oxidation of pharmaceuticals Endocrine Disruptors and pesticides during drinking water treatment
Water Research, 2009Co-Authors: Romain Broseus, Simon Vincent, Khadija Aboulfadl, Atlasi Daneshvar, Sebastien Sauve, Benoit Barbeau, Michele PrevostAbstract:This study investigates the oxidation of pharmaceuticals, Endocrine disrupting compounds and pesticides during ozonation applied in drinking water treatment. In the first step, second-order rate constants for the reactions of selected compounds with molecular ozone (k(O3)) were determined in bench-scale experiments at pH 8.10: caffeine (650+/-22M(-1)s(-1)), progesterone (601+/-9M(-1)s(-1)), medroxyprogesterone (558+/-9M(-1)s(-1)), norethindrone (2215+/-76M(-1)s(-1)) and levonorgestrel (1427+/-62M(-1)s(-1)). Compared to phenolic estrogens (estrone, 17beta-estradiol, estriol and 17alpha-ethinylestradiol), the selected progestogen Endocrine Disruptors reacted far slower with ozone. In the second part of the study, bench-scale experiments were conducted with surface waters spiked with 16 target compounds to assess their oxidative removal using ozone and determine if bench-scale results would accurately predict full-scale removal data. Overall, the data provided evidence that ozone is effective for removing trace organic contaminants from water with ozone doses typically applied in drinking water treatment. Ozonation removed over 80% of caffeine, pharmaceuticals and Endocrine Disruptors within the CT value of about 2 mg min L(-1). As expected, pesticides were found to be the most recalcitrant compounds to oxidize. Caffeine can be used as an indicator compound to gauge the efficacy of ozone treatment.
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ozone oxidation of pharmaceuticals Endocrine Disruptors and pesticides during drinking water treatment
Water Research, 2009Co-Authors: Romain Broseus, Simon Vincent, Khadija Aboulfadl, Atlasi Daneshvar, Sebastien Sauve, Benoit Barbeau, Michele PrevostAbstract:Abstract This study investigates the oxidation of pharmaceuticals, Endocrine disrupting compounds and pesticides during ozonation applied in drinking water treatment. In the first step, second-order rate constants for the reactions of selected compounds with molecular ozone ( k O 3 ) were determined in bench-scale experiments at pH 8.10: caffeine (650 ± 22 M−1 s−1), progesterone (601 ± 9 M−1 s−1), medroxyprogesterone (558 ± 9 M−1 s−1), norethindrone (2215 ± 76 M−1 s−1) and levonorgestrel (1427 ± 62 M−1 s−1). Compared to phenolic estrogens (estrone, 17β-estradiol, estriol and 17α-ethinylestradiol), the selected progestogen Endocrine Disruptors reacted far slower with ozone. In the second part of the study, bench-scale experiments were conducted with surface waters spiked with 16 target compounds to assess their oxidative removal using ozone and determine if bench-scale results would accurately predict full-scale removal data. Overall, the data provided evidence that ozone is effective for removing trace organic contaminants from water with ozone doses typically applied in drinking water treatment. Ozonation removed over 80% of caffeine, pharmaceuticals and Endocrine Disruptors within the CT value of about 2 mg min L−1. As expected, pesticides were found to be the most recalcitrant compounds to oxidize. Caffeine can be used as an indicator compound to gauge the efficacy of ozone treatment.
Damia Barcelo - One of the best experts on this subject based on the ideXlab platform.
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determination of a broad spectrum of pharmaceuticals and Endocrine Disruptors in biofilm from a waste water treatment plant impacted river
Science of The Total Environment, 2016Co-Authors: Belinda Huerta, Sara Rodriguezmozaz, Christina Nannou, L Nakis, Albert Ruhi, Vicenc Acuna, Sergi Sabater, Damia BarceloAbstract:Abstract Wastewater treatment plants (WWTPs) are one of the main sources of pharmaceuticals and Endocrine disrupting compounds in freshwater ecosystems, and several studies have reported bioaccumulation of these compounds in different organisms in those ecosystems. River biofilms are exceptional indicators of pollution, but very few studies have focused on the accumulation of these emerging contaminants. The objectives of this study were first to develop an efficient analytical methodology for the simultaneous analysis of 44 pharmaceuticals and 13 Endocrine disrupting compounds in biofilm, and second, to assess persistence, distribution, and bioaccumulation of these contaminants in natural biofilms inhabiting a WWTP-impacted river. The method is based on pressurized liquid extraction, purification by solid-phase extraction, and analysis by ultra performance liquid chromatography coupled to a mass spectrometer (UPLC–MS/MS) in tandem. Recoveries for pharmaceuticals were 31–137%, and for Endocrine Disruptors 32–93%. Method detection limits for Endocrine Disruptors were in the range of 0.2–2.4 ng g − 1 , and for pharmaceuticals, 0.07–6.7 ng g − 1 . A total of five Endocrine Disruptors and seven pharmaceuticals were detected in field samples at concentrations up to 100 ng g − 1 .
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development of an extraction and purification method for the determination of multi class pharmaceuticals and Endocrine Disruptors in freshwater invertebrates
Talanta, 2015Co-Authors: Belinda Huerta, Sara Rodriguezmozaz, Albert Ruhi, Vicenc Acuna, Sergi Sabater, Anna Jakimska, Marta Llorca, G Margoutidis, Damia BarceloAbstract:Abstract Aquatic organisms from freshwater ecosystems impacted by waste water treatment plant (WWTP) effluents are constantly exposed to constant concentrations of pharmaceuticals, Endocrine Disruptors and related compounds, among other anthropogenic contaminants. Macroinvertebrates inhabiting freshwater ecosystems might be useful bioindicators of exposure to contaminants, since their lives are long enough to bioaccumulate, but at the same time may integrate short-term changes in the environment. However, studies about potential bioaccumulation of emerging contaminants in these organisms are very scarce. The objectives of this study were to develop an analytical methodology for the analysis of 41 pharmaceuticals and 21 Endocrine Disruptors in freshwater invertebrates. In addition, bioaccumulation of these contaminants in three macroinvertebrate taxa inhabiting a waste water treatment plant -impacted river was evaluated. The method for the simultaneous extraction of both families of compounds is based on sonication, purification via removal of phospholipids, and analysis by ultra performance liquid chromatography coupled to a mass spectrometer (UPLC–MS/MS) in tandem. Recoveries for pharmaceuticals were 34–125%, and for Endocrine Disruptors were 48–117%. Method detection limits (MDLs) for EDCs were in the range of 0.080–2.4 ng g−1, and for pharmaceuticals, 0.060–4.3 ng g−1. These pollutants were detected in water samples taken downstream the waste water treatment plant effluent at concentrations up to 572 ng L−1. Two non-esteroidal anti-inflammatory drugs, diclofenac and ibuprofen, and four Endocrine Disruptors – estrone, bisphenol A, TBEP, and nonylphenol – were detected in at least one macroinvertebrate taxa in concentrations up to 183 ng g−1 (dry weight). An isobaric interference was identified during the analysis of diclofenac in Hydropsyche samples, which was successfully discriminated via accurate mass determination by TFC-LTQ Orbitrap.
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monitoring of Endocrine Disruptors in surface waters by the yeast recombinant assay
Environmental Toxicology and Chemistry, 2001Co-Authors: Natalia Garciareyero, Damia Barcelo, Eulalia Grau, Montserrat Castillo, Maria Lopez J De Alda, Benjamin PinaAbstract:Endocrine Disruptors exert physiological effects at very low concentrations. Surface waters present often a mixture of high concentrations of low-potency Disruptors and low amounts of very powerful ones, making their chemical analysis complicated and expensive. We developed a recombinant yeast assay (RYA) for estrogenic compounds using 96-well microtiter plates. This assay is based on three yeast strains, transformed with self-propagating plasmids. One strain contains an expression plasmid for the human estrogen hormone receptor and an appropriate reporter; it detects estrogenic and antiestrogenic activities. The two other yeast strains, one expressing the human progesterone receptor and a second based on the yeast activator Gal4p, served to analyze the nature of antiestrogenic activities. We applied this technique to water samples from two tributaries of the Llobregat river (NE Spain) as well as from four sewage treatment plants discharging on them. Our results indicate that the efficiency of sewage treatment plants for eliminating estrogenic compounds varied notably, being in at least one case completely inefficient. We also observed a prevalence of an inhibitory activity all through the two rivers; this inhibition was hormone specific. These results were consistent to previously obtained chemical analyses of the same samples, although chemical and in vivo analyses showed rather different levels of sensitivity for some compounds. Our findings demonstrate the utility of the yeast recombinant assay for analyzing complex natural samples; at the same time, they stress the necessity of a panel of different yeast systems to adequately describe Endocrine-disruptor activities.
Nikhil R Jana - One of the best experts on this subject based on the ideXlab platform.
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reduced graphene oxide silver nanoparticle composite as visible light photocatalyst for degradation of colorless Endocrine Disruptors
ACS Applied Materials & Interfaces, 2014Co-Authors: Susanta Kumar Bhunia, Nikhil R JanaAbstract:Sunlight-induced degradation of organic pollutants is an ideal approach for environmental pollution control and wastewater treatment. Although a variety of photocatalysts have been designed toward this goal, efficient degradation of colorless organic pollutants by visible light is a challenging issue. Here, we show that a reduced graphene oxide (rGO)-based composite with silver nanoparticle (rGO–Ag) can act as an efficient visible-light photocatalyst for the degradation of colorless organic pollutants. We have developed a simple, large-scale synthesis method for rGO–Ag and used it for the degradation of three well-known Endocrine Disruptors (phenol, bisphenol A, and atrazine) under UV and visible light. It is found that photocatalytic efficiency by rGO–Ag under visible light is significantly higher compared to that of rGO or silver nanoparticles. It is proposed that Ag nanoparticles offer visible-light-induced excitation of silver plasmons, and conductive rGO offers efficient charge separation and thus in...
Romain Broseus - One of the best experts on this subject based on the ideXlab platform.
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ozone oxidation of pharmaceuticals Endocrine Disruptors and pesticides during drinking water treatment
Water Research, 2009Co-Authors: Romain Broseus, Simon Vincent, Khadija Aboulfadl, Atlasi Daneshvar, Sebastien Sauve, Benoit Barbeau, Michele PrevostAbstract:This study investigates the oxidation of pharmaceuticals, Endocrine disrupting compounds and pesticides during ozonation applied in drinking water treatment. In the first step, second-order rate constants for the reactions of selected compounds with molecular ozone (k(O3)) were determined in bench-scale experiments at pH 8.10: caffeine (650+/-22M(-1)s(-1)), progesterone (601+/-9M(-1)s(-1)), medroxyprogesterone (558+/-9M(-1)s(-1)), norethindrone (2215+/-76M(-1)s(-1)) and levonorgestrel (1427+/-62M(-1)s(-1)). Compared to phenolic estrogens (estrone, 17beta-estradiol, estriol and 17alpha-ethinylestradiol), the selected progestogen Endocrine Disruptors reacted far slower with ozone. In the second part of the study, bench-scale experiments were conducted with surface waters spiked with 16 target compounds to assess their oxidative removal using ozone and determine if bench-scale results would accurately predict full-scale removal data. Overall, the data provided evidence that ozone is effective for removing trace organic contaminants from water with ozone doses typically applied in drinking water treatment. Ozonation removed over 80% of caffeine, pharmaceuticals and Endocrine Disruptors within the CT value of about 2 mg min L(-1). As expected, pesticides were found to be the most recalcitrant compounds to oxidize. Caffeine can be used as an indicator compound to gauge the efficacy of ozone treatment.
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ozone oxidation of pharmaceuticals Endocrine Disruptors and pesticides during drinking water treatment
Water Research, 2009Co-Authors: Romain Broseus, Simon Vincent, Khadija Aboulfadl, Atlasi Daneshvar, Sebastien Sauve, Benoit Barbeau, Michele PrevostAbstract:Abstract This study investigates the oxidation of pharmaceuticals, Endocrine disrupting compounds and pesticides during ozonation applied in drinking water treatment. In the first step, second-order rate constants for the reactions of selected compounds with molecular ozone ( k O 3 ) were determined in bench-scale experiments at pH 8.10: caffeine (650 ± 22 M−1 s−1), progesterone (601 ± 9 M−1 s−1), medroxyprogesterone (558 ± 9 M−1 s−1), norethindrone (2215 ± 76 M−1 s−1) and levonorgestrel (1427 ± 62 M−1 s−1). Compared to phenolic estrogens (estrone, 17β-estradiol, estriol and 17α-ethinylestradiol), the selected progestogen Endocrine Disruptors reacted far slower with ozone. In the second part of the study, bench-scale experiments were conducted with surface waters spiked with 16 target compounds to assess their oxidative removal using ozone and determine if bench-scale results would accurately predict full-scale removal data. Overall, the data provided evidence that ozone is effective for removing trace organic contaminants from water with ozone doses typically applied in drinking water treatment. Ozonation removed over 80% of caffeine, pharmaceuticals and Endocrine Disruptors within the CT value of about 2 mg min L−1. As expected, pesticides were found to be the most recalcitrant compounds to oxidize. Caffeine can be used as an indicator compound to gauge the efficacy of ozone treatment.