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Paul Lambrechts - One of the best experts on this subject based on the ideXlab platform.

  • regenerative Endodontic Procedure of immature permanent teeth with leukocyte and platelet rich fibrin a multicenter controlled clinical trial
    Journal of Endodontics, 2021
    Co-Authors: Nastaran Meschi, Gertrude Van Gorp, Mostafa Ezeldeen, Andres Eduardo Torres Garcia, Pierre Lahoud, Wim Coucke, Reinhilde Jacobs, Katleen Vandamme, Wim Teughels, Paul Lambrechts
    Abstract:

    AIM The aim of this non-randomized, multicenter controlled clinical trial was to evaluate the impact of leukocyte and platelet rich fibrin (LPRF) on regenerative Endodontic Procedures (REP) of immature permanent teeth in terms of peri-apical bone healing (PBH) and further root development (RD). METHODOLOGY Healthy patients between 6-25 years with an inflamed or necrotic immature permanent tooth were included and divided between the test (= REP+LPRF) and control (= REP-LPRF) group, depending on their compliance and the clinical setting (university hospital or private practice). After receiving REP+/-LPRF, the patients were recalled after 3, 6, 12, 24 and 36 months. At each recall session the teeth were clinically and radiographically (by means of a peri-apical radiograph (PR)) evaluated. A cone beam computed tomography (CBCT) was taken pre-operatively, 2 and 3 years post-operatively. PBH and RD were quantitatively and qualitatively assessed. RESULTS Twenty-nine teeth with a necrotic pulp were included from which 23 (9 test, 14 control) were analyzed. Three teeth in the test group had a flare-up reaction in the first year post REP. Except for 2 no shows, all the analyzed teeth survived up to 3 years post REP and in case of failure apexification preserved them. Complete PBH was obtained in 91.3% and 87% of the cases, based on PR qualitative and quantitative evaluation respectively, with no significant difference between the groups with respect to (wrt) the baseline. The PR quantitative change in RD at the last recall session, wrt the baseline, was not significant (all p-values >0.05) in both groups. The qualitative assessment of the type of REP root healing was non-uniform. In the test group 55.6% of the teeth presented no RD and no apical closure. Only 50% of the 14 teeth assessed with CBCT presented complete PBH. Regarding volumetric measurements on RD 3 years post REP, for the change wrt the baseline in root hard tissue volume (RHTV), mean root hard tissue thickness (RHTT) and apical area, the control group performed significantly in favor of RD than the test group (p= 0.03, 0.003 and 0.05 respectively). For the volumetric change 3 years post REP wrt the baseline in root length and maximum RHTT, no significant difference (p= 0.72 and 0.4 respectively) was found between the groups. The correlation between the PR and CBCT variables assessing RD was weak (root lengthening) to very weak (root thickening). CONCLUSION REP-LPRF seems to be a viable treatment option to obtain PBH and aid further RD of necrotic immature permanent teeth. Caution is needed when evaluating REP with PR.

  • a retrospective case series in regenerative Endodontics trend analysis based on clinical evaluation and 2 and 3 dimensional radiology
    Journal of Endodontics, 2018
    Co-Authors: Nastaran Meschi, Mostafa Ezeldeen, Andres Eduardo Torres Garcia, Reinhilde Jacobs, Paul Lambrechts
    Abstract:

    Abstract Introduction A regenerative Endodontic Procedure (REP) is a biologically based treatment to functionally replace the pulp of infected immature permanent teeth. The purpose of this retrospective case series was to assess the outcome of REPs of infected immature permanent teeth in terms of periapical bone healing (PBH), root development (RD), and pulp vitality. Methods Five patients (1 tooth/patient) who had undergone a REP based on the cell homing concept were recalled 3, 6, 12, 24, and 36 months postoperatively. At each recall session, clinical and periapical radiographic (PR) investigations were performed. Cone-beam computed tomographic (CBCT) imaging was taken before and 36 months after REPs. Qualitative and quantitative PR assessments were performed on the teeth that underwent REPs. Quantitative CBCT analyses were performed on the teeth that underwent REP and contralateral teeth. Results At each recall session, all teeth were asymptomatic but reacted negatively on carbon dioxide snow and electrical pulp testing. All teeth that underwent a REP showed complete PBH and further RD on PR and CBCT assessments when comparing the baseline with the final recall radiographs. CBCT analyses indicated increases in root hard tissue volume and RL for all teeth that underwent a REP, but they were 5 and 3 times less, respectively, than the contralateral teeth. The postoperative CBCT images presented bone ingrowth inside the root canal, calcification, or nonuniform RD. Conclusions Thirty-six months after the REPs (based on the cell homing concept), this RCS resulted radiographically and clinically in functional and asymptomatic teeth with complete PBH and continued reparative RD. CBCT quantitative measurements and qualitative root development observations are more reliable and accurate than PR analysis.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Kathleen Van Den Eynde, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    Objectives An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Kathleen Van Den Eynde, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated. A REP was performed on tooth 45 of a 10-year-old girl. Eleven months post-treatment, the tooth had to be removed for orthodontic reasons. The following investigations were performed: immunohistology and radiographic quantification of root development. After hematoxylin-eosin (HE) staining, the following immunomarkers were selected: neurofilament (NF), pan cytokeratin (PK), osteocalcin (OC), and CD34. The REP resulted in clinical and radiographic healing of the periradicular lesion and quantifiable root development. The HE staining matches with the medical imaging post-REP: underneath the mineral trioxide aggregate a calcified bridge with cell inclusions, connective pulp-like tissue (PLT) with blood vessels, osteodentin against the root canal walls, on the root surface cementum (Ce), and periodontal ligament (PDL). The PDL was PK+. The blood vessels in the PLT and PDL were CD34+. The Ce, osteodentin, and stromal cells in the PLT were OC+. The neurovascular bundles in the PLT were NF+. Immunohistologically, REP of this infected immature permanent tooth resulted in an intracanalar connective tissue with a regulated physiology, but not pulp tissue. REP of an immature permanent infected tooth may heal the periapical infection and may result in a combination of regeneration and repair of the pulp-dentin complex.

Nastaran Meschi - One of the best experts on this subject based on the ideXlab platform.

  • regenerative Endodontic Procedure of immature permanent teeth with leukocyte and platelet rich fibrin a multicenter controlled clinical trial
    Journal of Endodontics, 2021
    Co-Authors: Nastaran Meschi, Gertrude Van Gorp, Mostafa Ezeldeen, Andres Eduardo Torres Garcia, Pierre Lahoud, Wim Coucke, Reinhilde Jacobs, Katleen Vandamme, Wim Teughels, Paul Lambrechts
    Abstract:

    AIM The aim of this non-randomized, multicenter controlled clinical trial was to evaluate the impact of leukocyte and platelet rich fibrin (LPRF) on regenerative Endodontic Procedures (REP) of immature permanent teeth in terms of peri-apical bone healing (PBH) and further root development (RD). METHODOLOGY Healthy patients between 6-25 years with an inflamed or necrotic immature permanent tooth were included and divided between the test (= REP+LPRF) and control (= REP-LPRF) group, depending on their compliance and the clinical setting (university hospital or private practice). After receiving REP+/-LPRF, the patients were recalled after 3, 6, 12, 24 and 36 months. At each recall session the teeth were clinically and radiographically (by means of a peri-apical radiograph (PR)) evaluated. A cone beam computed tomography (CBCT) was taken pre-operatively, 2 and 3 years post-operatively. PBH and RD were quantitatively and qualitatively assessed. RESULTS Twenty-nine teeth with a necrotic pulp were included from which 23 (9 test, 14 control) were analyzed. Three teeth in the test group had a flare-up reaction in the first year post REP. Except for 2 no shows, all the analyzed teeth survived up to 3 years post REP and in case of failure apexification preserved them. Complete PBH was obtained in 91.3% and 87% of the cases, based on PR qualitative and quantitative evaluation respectively, with no significant difference between the groups with respect to (wrt) the baseline. The PR quantitative change in RD at the last recall session, wrt the baseline, was not significant (all p-values >0.05) in both groups. The qualitative assessment of the type of REP root healing was non-uniform. In the test group 55.6% of the teeth presented no RD and no apical closure. Only 50% of the 14 teeth assessed with CBCT presented complete PBH. Regarding volumetric measurements on RD 3 years post REP, for the change wrt the baseline in root hard tissue volume (RHTV), mean root hard tissue thickness (RHTT) and apical area, the control group performed significantly in favor of RD than the test group (p= 0.03, 0.003 and 0.05 respectively). For the volumetric change 3 years post REP wrt the baseline in root length and maximum RHTT, no significant difference (p= 0.72 and 0.4 respectively) was found between the groups. The correlation between the PR and CBCT variables assessing RD was weak (root lengthening) to very weak (root thickening). CONCLUSION REP-LPRF seems to be a viable treatment option to obtain PBH and aid further RD of necrotic immature permanent teeth. Caution is needed when evaluating REP with PR.

  • a retrospective case series in regenerative Endodontics trend analysis based on clinical evaluation and 2 and 3 dimensional radiology
    Journal of Endodontics, 2018
    Co-Authors: Nastaran Meschi, Mostafa Ezeldeen, Andres Eduardo Torres Garcia, Reinhilde Jacobs, Paul Lambrechts
    Abstract:

    Abstract Introduction A regenerative Endodontic Procedure (REP) is a biologically based treatment to functionally replace the pulp of infected immature permanent teeth. The purpose of this retrospective case series was to assess the outcome of REPs of infected immature permanent teeth in terms of periapical bone healing (PBH), root development (RD), and pulp vitality. Methods Five patients (1 tooth/patient) who had undergone a REP based on the cell homing concept were recalled 3, 6, 12, 24, and 36 months postoperatively. At each recall session, clinical and periapical radiographic (PR) investigations were performed. Cone-beam computed tomographic (CBCT) imaging was taken before and 36 months after REPs. Qualitative and quantitative PR assessments were performed on the teeth that underwent REPs. Quantitative CBCT analyses were performed on the teeth that underwent REP and contralateral teeth. Results At each recall session, all teeth were asymptomatic but reacted negatively on carbon dioxide snow and electrical pulp testing. All teeth that underwent a REP showed complete PBH and further RD on PR and CBCT assessments when comparing the baseline with the final recall radiographs. CBCT analyses indicated increases in root hard tissue volume and RL for all teeth that underwent a REP, but they were 5 and 3 times less, respectively, than the contralateral teeth. The postoperative CBCT images presented bone ingrowth inside the root canal, calcification, or nonuniform RD. Conclusions Thirty-six months after the REPs (based on the cell homing concept), this RCS resulted radiographically and clinically in functional and asymptomatic teeth with complete PBH and continued reparative RD. CBCT quantitative measurements and qualitative root development observations are more reliable and accurate than PR analysis.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Kathleen Van Den Eynde, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    Objectives An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Kathleen Van Den Eynde, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated. A REP was performed on tooth 45 of a 10-year-old girl. Eleven months post-treatment, the tooth had to be removed for orthodontic reasons. The following investigations were performed: immunohistology and radiographic quantification of root development. After hematoxylin-eosin (HE) staining, the following immunomarkers were selected: neurofilament (NF), pan cytokeratin (PK), osteocalcin (OC), and CD34. The REP resulted in clinical and radiographic healing of the periradicular lesion and quantifiable root development. The HE staining matches with the medical imaging post-REP: underneath the mineral trioxide aggregate a calcified bridge with cell inclusions, connective pulp-like tissue (PLT) with blood vessels, osteodentin against the root canal walls, on the root surface cementum (Ce), and periodontal ligament (PDL). The PDL was PK+. The blood vessels in the PLT and PDL were CD34+. The Ce, osteodentin, and stromal cells in the PLT were OC+. The neurovascular bundles in the PLT were NF+. Immunohistologically, REP of this infected immature permanent tooth resulted in an intracanalar connective tissue with a regulated physiology, but not pulp tissue. REP of an immature permanent infected tooth may heal the periapical infection and may result in a combination of regeneration and repair of the pulp-dentin complex.

Petra Hilkens - One of the best experts on this subject based on the ideXlab platform.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Kathleen Van Den Eynde, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    Objectives An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Kathleen Van Den Eynde, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated. A REP was performed on tooth 45 of a 10-year-old girl. Eleven months post-treatment, the tooth had to be removed for orthodontic reasons. The following investigations were performed: immunohistology and radiographic quantification of root development. After hematoxylin-eosin (HE) staining, the following immunomarkers were selected: neurofilament (NF), pan cytokeratin (PK), osteocalcin (OC), and CD34. The REP resulted in clinical and radiographic healing of the periradicular lesion and quantifiable root development. The HE staining matches with the medical imaging post-REP: underneath the mineral trioxide aggregate a calcified bridge with cell inclusions, connective pulp-like tissue (PLT) with blood vessels, osteodentin against the root canal walls, on the root surface cementum (Ce), and periodontal ligament (PDL). The PDL was PK+. The blood vessels in the PLT and PDL were CD34+. The Ce, osteodentin, and stromal cells in the PLT were OC+. The neurovascular bundles in the PLT were NF+. Immunohistologically, REP of this infected immature permanent tooth resulted in an intracanalar connective tissue with a regulated physiology, but not pulp tissue. REP of an immature permanent infected tooth may heal the periapical infection and may result in a combination of regeneration and repair of the pulp-dentin complex.

Ivo Lambrichts - One of the best experts on this subject based on the ideXlab platform.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Kathleen Van Den Eynde, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    Objectives An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Kathleen Van Den Eynde, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated. A REP was performed on tooth 45 of a 10-year-old girl. Eleven months post-treatment, the tooth had to be removed for orthodontic reasons. The following investigations were performed: immunohistology and radiographic quantification of root development. After hematoxylin-eosin (HE) staining, the following immunomarkers were selected: neurofilament (NF), pan cytokeratin (PK), osteocalcin (OC), and CD34. The REP resulted in clinical and radiographic healing of the periradicular lesion and quantifiable root development. The HE staining matches with the medical imaging post-REP: underneath the mineral trioxide aggregate a calcified bridge with cell inclusions, connective pulp-like tissue (PLT) with blood vessels, osteodentin against the root canal walls, on the root surface cementum (Ce), and periodontal ligament (PDL). The PDL was PK+. The blood vessels in the PLT and PDL were CD34+. The Ce, osteodentin, and stromal cells in the PLT were OC+. The neurovascular bundles in the PLT were NF+. Immunohistologically, REP of this infected immature permanent tooth resulted in an intracanalar connective tissue with a regulated physiology, but not pulp tissue. REP of an immature permanent infected tooth may heal the periapical infection and may result in a combination of regeneration and repair of the pulp-dentin complex.

Athina Mavridou - One of the best experts on this subject based on the ideXlab platform.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Kathleen Van Den Eynde, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    Objectives An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated.

  • regenerative Endodontic Procedure of an infected immature permanent human tooth an immunohistological study
    Clinical Oral Investigations, 2016
    Co-Authors: Nastaran Meschi, Petra Hilkens, Ivo Lambrichts, Athina Mavridou, Olaf Strijbos, Marieke De Ketelaere, Kathleen Van Den Eynde, Gertrude Van Gorp, Paul Lambrechts
    Abstract:

    An immunohistological study of an infected immature permanent human tooth after a regenerative Endodontic Procedure (REP) was conducted in order to determine the histologic outcome of this Procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated. A REP was performed on tooth 45 of a 10-year-old girl. Eleven months post-treatment, the tooth had to be removed for orthodontic reasons. The following investigations were performed: immunohistology and radiographic quantification of root development. After hematoxylin-eosin (HE) staining, the following immunomarkers were selected: neurofilament (NF), pan cytokeratin (PK), osteocalcin (OC), and CD34. The REP resulted in clinical and radiographic healing of the periradicular lesion and quantifiable root development. The HE staining matches with the medical imaging post-REP: underneath the mineral trioxide aggregate a calcified bridge with cell inclusions, connective pulp-like tissue (PLT) with blood vessels, osteodentin against the root canal walls, on the root surface cementum (Ce), and periodontal ligament (PDL). The PDL was PK+. The blood vessels in the PLT and PDL were CD34+. The Ce, osteodentin, and stromal cells in the PLT were OC+. The neurovascular bundles in the PLT were NF+. Immunohistologically, REP of this infected immature permanent tooth resulted in an intracanalar connective tissue with a regulated physiology, but not pulp tissue. REP of an immature permanent infected tooth may heal the periapical infection and may result in a combination of regeneration and repair of the pulp-dentin complex.