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G Gasbarrini - One of the best experts on this subject based on the ideXlab platform.

Magnus Strom - One of the best experts on this subject based on the ideXlab platform.

  • antibodies against deamidated gliadin peptides identify adult coeliac disease patients negative for antibodies against Endomysium and tissue transglutaminase
    Alimentary Pharmacology & Therapeutics, 2010
    Co-Authors: Charlotte Dahle, A Hagman, Simone Ignatova, Magnus Strom
    Abstract:

    Background This study was done to evaluate the diagnostic utility of antibodies against deamidated gliadin peptides compared to traditional markers for coeliac disease. Aim To evaluate diagnostic utility of antibodies against deamidated gliadin peptide (DGP). Methods Sera from 176 adults, referred for endoscopy without previous analysis of antibodies against tissue transglutaminase (tTG) or Endomysium (EmA), were retrospectively analysed by ELISAs detecting IgA/IgG antibodies against DGP or a mixture of DGP and tTG, and compared with IgA-tTG and EmA. Seventy-nine individuals were diagnosed with coeliac disease. Results Receiver operating characteristic analyses verified the manufacturers cut-off limits except for IgA/IgG-DGP/ tTG. In sera without IgA deficiency, the sensitivity was higher for IgA/IgG-DGP (0.85-0.87) compared with IgA-tTg (0.76) and EmA (0.61). All tests showed high specificity (0.95-1.00). Eighteen coeliac disease-sera were negative regarding IgA-tTG, nine of which were positive for IgA/IgG-DGP. Sera from coeliac disease-patients greater than70 years were more often negative for IgA-tTG (50%) and IgA/IgG-DGP (36%) than younger patients (15% and 8% respectively) (P less than 0.01). Three of the four IgA-deficient patients were positive in the IgA/IgG-DGP assay. Conclusions In this study of patients unselected regarding IgA-tTg/EmA, thus unbiased in this respect, IgA/IgG-DGP identified adult coeliac disease patients negative for antibodies against Endomysium and tissue transglutaminase. Serology is often negative in elderly patients with coeliac disease; a small bowel biopsy should therefore be performed generously before coeliac disease is excluded.

  • Is small bowel biopsy necessary in adults with suspected celiac disease and IgA anti-Endomysium antibodies? 100% positive predictive value for celiac disease in adults.
    Digestive Diseases and Sciences, 1996
    Co-Authors: T Valdimarsson, Lennart Franzen, Ewa Grodzinsky, Thomas Skogh, Magnus Strom
    Abstract:

    The comparative diagnostic value of IgA anti-Endomysium and IgA antigliadin antibodies in adults with histologically confirmed celiac disease is reported. Sera from 144 adult patients (without concurrent dermatitis herpetiformis or IgA deficiency) who underwent small bowel biopsy were analyzed for both IgA anti-Endomysium and IgA anti-gliadin antibodies. Nineteen patients (13%) had celiac disease. The presence of IgA antiEndomysium antibodies had a sensitivity of 74% and a specificity of 100%. The positive and negative predictive values were 100% and 96%, respectively, and the diagnostic accuracy was 97%. In contrast, IgA anti-gliadin antibodies had positive and negative predictive values of 28% and 96%, respectively, with a diagnostic accuracy of 71%. Based on these data, we suggest that small bowel biopsy is not necessary to diagnose celiac disease in symptomatic adults with IgA antiEndomysium antibodies. Due to a negative predictive value of 96%, some symptomatic adults lacking anti-Endomysium antibodies will not be correctly diagnosed without small bowel biopsy.

  • is small bowel biopsy necessary in adults with suspected celiac disease and iga anti Endomysium antibodies 100 positive predictive value for celiac disease in adults
    Digestive Diseases and Sciences, 1996
    Co-Authors: T Valdimarsson, Lennart Franzen, Ewa Grodzinsky, Thomas Skogh, Magnus Strom
    Abstract:

    The comparative diagnostic value of IgA anti-Endomysium and IgA antigliadin antibodies in adults with histologically confirmed celiac disease is reported. Sera from 144 adult patients (without concurrent dermatitis herpetiformis or IgA deficiency) who underwent small bowel biopsy were analyzed for both IgA anti-Endomysium and IgA anti-gliadin antibodies. Nineteen patients (13%) had celiac disease. The presence of IgA antiEndomysium antibodies had a sensitivity of 74% and a specificity of 100%. The positive and negative predictive values were 100% and 96%, respectively, and the diagnostic accuracy was 97%. In contrast, IgA anti-gliadin antibodies had positive and negative predictive values of 28% and 96%, respectively, with a diagnostic accuracy of 71%. Based on these data, we suggest that small bowel biopsy is not necessary to diagnose celiac disease in symptomatic adults with IgA antiEndomysium antibodies. Due to a negative predictive value of 96%, some symptomatic adults lacking anti-Endomysium antibodies will not be correctly diagnosed without small bowel biopsy.

Antonio Tursi - One of the best experts on this subject based on the ideXlab platform.

Koui Takahashi - One of the best experts on this subject based on the ideXlab platform.

  • Structural weakening of intramuscular connective tissue during postmortem aging of pork
    Animal Science Journal, 2008
    Co-Authors: Takanori Nishimura, Suhong Fang, Jun-ichi Wakamatsu, Koui Takahashi
    Abstract:

    We studied structural changes in the Endomysium and perimysium during postmortem aging of pork using the cell-maceration/scanning electron microscope method. Immediately post mortem, endomysia sheaths that house individual muscle fibers displayed a honeycomb-like structure. The sheaths of the Endomysium consisted of tightly arranged collagen fibrils in a random network. The perimysium comprised several layers of wavy sheets made up of tightly bundled collagen fibers. While the structure of the intramuscular connective tissues remained almost unchanged up to five days post mortem, the Endomysium had resolved into individual collagen fibrils, and the thick sheets of the perimysium had separated into collagen fibers and fibrils at 8 days post mortem. These results provide direct evidence for structural weakening of the Endomysium and perimysium during postmortem aging of pork. The shear-force value of raw pork decreased rapidly within six days post mortem and then decreased slowly until 14 days post mortem. Since the rapid increase in tenderness is mainly due to structural weakening of myofibrils, we conclude that the disintegration of the Endomysium and perimysium contributes to tenderization of pork during extended postmortem aging.

  • Structural changes in Endomysium and perimysium during post-mortem aging of chicken Semitendinosus muscle—Contribution of structural weakening of intramuscular connective tissue to meat tenderization
    Meat Science, 2003
    Co-Authors: Takanori Nishimura, Koui Takahashi
    Abstract:

    Post-mortem changes in Endomysium and perimysium were investigated during aging of chicken semitendinosus muscle at 4°C. Although the shear-force value of raw meat decreased rapidly within 5 h post mortem and gradually thereafter, the solubility of collagen and the ratio of each chain of soluble collagen remained unchanged during 24 h post mortem. Light microscopic studies showed that structures of Endomysium and perimysium disintegrated into several thin sheets within 12 h post mortem, and that many gaps opened in the cross-section of Endomysium and perimysium. While Endomysium and perimysium were not stained by periodic acid Schiff reagent in fresh muscle, they were markedly stained in muscle 12 h post mortem. These results provide direct evidence for the structural weakening of Endomysium and perimysium during post-mortem aging of chicken. Therefore, we conclude that the structural weakening of the intramuscular connective tissue is closely related to tenderization of chicken.

  • Structural weakening of intramuscular connective tissue during conditioning of beef.
    Meat Science, 2002
    Co-Authors: Takanori Nishimura, Akihito Hattori, Koui Takahashi
    Abstract:

    Abstract The structural changes in intramuscular connective tissues Endomysium and perimysium during conditioning of beef were investigated using an improved technique of scanning electron microscopy. In beef conditioned for 28 days of 4°C, the Endomysium resolved into individual collagen fibrils and the thick sheets of perimysium separated into collagen fibres of 4–8 μm in diameter. These results provide direct evidence for the structural weakening of Endomysium and perimysium during conditioning. The structural changes in the intramuscular connective tissue were minimal until 10 days post mortem, but clearly observable after 14 days post mortem . Therefore, it is concluded that intramuscular connective tissue shows the effect on tenderisation of extended conditioning (2–4 weeks) of beef.

  • Structural weakening of intramuscular connective tissue during Post Mortem ageing of chicken Semitendinosus muscle
    Meat Science, 2002
    Co-Authors: Takanori Nishimura, Koui Takahashi
    Abstract:

    Abstract The structural changes in intramuscular connective tissues Endomysium and perimysium during post mortem ageing in chicken semitendinosus muscle were investigated using an improved technique of scanning electron microscopy. In post mortem chicken aged for 12 h at 4°C, the Endomysium resolved into individual collagen fibrils and the perimysial sheets separated into collagen fibres. These results provide direct evidence for the structural weakening of Endomysium and perimysium during post mortem ageing. The structural changes in the intramuscular connective tissue were minimal until 6 h post mortem, but clearly observable after 12 h post mortem. It was concluded that this disintegration of the intramuscular connective tissue is the chief mechanism in tenderisation during extended post mortem ageing of chicken.

  • Developmental expression of extracellular matrix components in intramuscular connective tissue of bovine semitendinosus muscle.
    Histochemistry and Cell Biology, 1997
    Co-Authors: Takanori Nishimura, Kouichi Ojima, Akihito Hattori, Koui Takahashi
    Abstract:

    We have investigated the expression patterns of extracellular matrix components in intramuscular connective tissue during the development of bovine semitendinosus muscle by means of indirect immunofluorescence techniques. Types I, III, V, and VI collagen and fibronectin were located in the Endomysium and the perimysium. Type IV collagen, laminin, and heparan sulfate proteoglycans (PGs) were exclusively located in the Endomysium, and dermatan sulfate PGs existed only in the perimysium. The localization of these components in the intramuscular connective tissue of semitendinosus muscle remained unchanged throughout prenatal and postnatal growth of cattle, suggesting that they are essential for forming and maintaining structures of the Endomysium and perimysium in bovine semitendinosus muscle. On the other hand, decorin was undetectable in the Endomysium of neonates, although other matrix components were already expressed. It was expressed slightly in the Endomysium of 2-month-old calves, and clearly detectable in the Endomysium of cattle more than 6 months old. Chondroitin sulfate PGs were barely detectable in the perimysium of fetuses and neonatal calves, and progressively appeared during postnatal development of the muscle. It seems likely that these PGs are closely related to the postnatal development of the Endomysium and perimysium.

Domenico Lombardi - One of the best experts on this subject based on the ideXlab platform.