The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
G.o.n. Oosterhof - One of the best experts on this subject based on the ideXlab platform.
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The influence of high-Energy Shock waves on the development of metastases
Ultrasound in medicine & biology, 1996Co-Authors: G.o.n. Oosterhof, Erik B. Cornel, G.a.h.j. Smits, Frans M.j. Debruyne, Jack A. SchalkenAbstract:Abstract The hypothesis that exposure of a solid tumor to high-Energy Shock waves (HESW) could lead to an increase of metastases was investigated in an animal model. The highly metastatic AT-6 Dunning R3327 rat prostate cancer subline was implanted in the hind limb of a Fisher-Copenhagen rat and was exposed to 6000 Shock waves delivered by an experimental lithotripter, or sham-treated, as soon as the tumor had reached a volume of 175–225 mm3. The tumor-bearing leg was amputated 24 h later and the number of metastases was examined 12 weeks thereafter at autopsy. Metastases were seen in 82% of the animals exposed to HESW and in 25% of the sham-treated animals. There was no significant difference in weight of the lungs that contained metastases, between sham and treated animals. These results were confirmed in a second experiment. We conclude that the metastatic spread of tumors with a high metastatic potential may be enhanced by Shock-wave exposure.
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THE EFFECTS OF SUCCESSIVE HIGH-Energy Shock-WAVE TUMOR ADMINISTRATION ON TUMOR BLOOD FLOW
Ultrasound in medicine & biology, 1995Co-Authors: Erik B. Cornel, G.a.h.j. Smits, Frans M.j. Debruyne, Arend Heerschap, Jack A. Schalken, G.o.n. OosterhofAbstract:The effects of repeated high-Energy Shock wave (HESW) tumor administration on tumor blood flow (TBF) were studied in NU-1 human kidney cancer xenografts. Deuteriated water was used as a magnetic resonance spectroscopic detectable tracer for measuring tumor blood flow. Tumors were exposed twice to 800 electromagnetically generated HESW, with a 24-h interval or sham exposed. No changes in TBF occurred after sham exposure to HESW. TBF levels 2 h after the first and second HESW application were, respectively, 46% and 37% lower than the mean preexposure TBF value and returned to normal levels within 16 h. There was statistically no difference found between the effects on tumor blood flow after the first and second HESW exposure. These observations are in agreement with earlier studies and provide a rationale to shorten the time interval between HESW monotreatments to 2 to 3 h.
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High Energy Shock Waves Induced Increase in the Local Concentration of Systemically Given TNF-α
The Journal of Urology, 1994Co-Authors: Erik B. Cornel, Frans M.j. Debruyne, Jack A. Schalken, Egbert Oosterwijk, J.d. Van De Streek, G. Grutters, G.o.n. OosterhofAbstract:The influence of high Energy Shock waves (HESW) on the local concentration of systemically given TNF-alpha was studied in a syngeneic rat bladder tumor model. 125I-TNF-alpha was injected intravenously and within 1 minute was followed by HESW tumor exposure. High Energy Shock waves were generated using an experimental Shock wave emitter based on the Siemens Lithostar Plus. Administration of HESW to RBT323 tumors resulted in a 3-fold increase in uptake of 125I-TNF-alpha in the tumor compared with nontreated tumors, whereas the tissue distribution and pharmacokinetics of nontumor tissue were similar irrespective of HESW exposure. These results demonstrate that HESW administration leads to an increase in the local concentration of a systemically given drug. The capacity of HESW to increase local drug concentrations will be of importance in designing more effective combined treatment strategies.
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High Energy Shock waves induced increase in the local concentration of systemically given TNF-alpha.
The Journal of urology, 1994Co-Authors: E B Cornel, Jack A. Schalken, Egbert Oosterwijk, J.d. Van De Streek, G. Grutters, F M Debruyne, G.o.n. OosterhofAbstract:The influence of high Energy Shock waves (HESW) on the local concentration of systemically given TNF-alpha was studied in a syngeneic rat bladder tumor model. 125I-TNF-alpha was injected intravenously and within 1 minute was followed by HESW tumor exposure. High Energy Shock waves were generated using an experimental Shock wave emitter based on the Siemens Lithostar Plus. Administration of HESW to RBT323 tumors resulted in a 3-fold increase in uptake of 125I-TNF-alpha in the tumor compared with nontreated tumors, whereas the tissue distribution and pharmacokinetics of nontumor tissue were similar irrespective of HESW exposure. These results demonstrate that HESW administration leads to an increase in the local concentration of a systemically given drug. The capacity of HESW to increase local drug concentrations will be of importance in designing more effective combined treatment strategies.
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Magnetic Resonance Spectroscopic Evaluation of the Effects of High-Energy Shock Waves Administered to a Human Kidney Cancer Xenograft
Magnetic Resonance in Nephrourology, 1993Co-Authors: Arend Heerschap, G.a.h.j. Smits, G.o.n. Oosterhof, H. J. Van Den Boogert, F. M. J. Debruyne, J. H. J. Ruijs, Jack A. SchalkenAbstract:The administration of high-Energy Shock waves (HESW) in clinical lithotripsy for kidney stone fragmentation is well known [1], but recent studies have revealed other potential biomedical applications for this type of acoustic Energy. It has been known for a few years that HESW can be used to suppress tumor growth in vivo [2]; a temporary suppression of tumor growth for different xenografts after focal administration of HESW has been demonstrated [3]. However, the processes underlying this biological effect are poorly understood. HESW may affect tumor cells and their metabolism both directly and indirectly (for example, at the vascular level).
Zhongcheng Xin - One of the best experts on this subject based on the ideXlab platform.
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low Energy Shock wave therapy ameliorates erectile dysfunction in a pelvic neurovascular injuries rat model
The Journal of Sexual Medicine, 2016Co-Authors: Melanie P Matheu, Fionna Sun, Lin Wang, Melissa T Sanford, Hongxiu Ning, Lia Banie, Yungchin Lee, Zhongcheng XinAbstract:Abstract Introduction Erectile dysfunction (ED) caused by pelvic injuries is a common complication of civil and battlefield trauma with multiple neurovascular factors involved, and no effective therapeutic approach is available. Aims To test the effect and mechanisms of low-Energy Shock wave (LESW) therapy in a rat ED model induced by pelvic neurovascular injuries. Methods Thirty-two male Sprague-Dawley rats injected with 5-ethynyl-2′-deoxyuridine (EdU) at newborn were divided into 4 groups: sham surgery (Sham), pelvic neurovascular injury by bilateral cavernous nerve injury and internal pudendal bundle injury (PVNI), PVNI treated with LESW at low Energy (Low), and PVNI treated with LESW at high Energy (High). After LESW treatment, rats underwent erectile function measurement and the tissues were harvested for histologic and molecular study. To examine the effect of LESW on Schwann cells, in vitro studies were conducted. Main Outcome Measurements The intracavernous pressure (ICP) measurement, histological examination, and Western blot (WB) were conducted. Cell cycle, Schwann cell activation-related markers were examined in in vitro experiments. Results LESW treatment improves erectile function in a rat model of pelvic neurovascular injury by leading to angiogenesis, tissue restoration, and nerve generation with more endogenous EdU + progenitor cells recruited to the damaged area and activation of Schwann cells. LESW facilitates more complete re-innervation of penile tissue with regeneration of neuronal nitric oxide synthase (nNOS)-positive nerves from the MPG to the penis. In vitro experiments demonstrated that LESW has a direct effect on Schwann cell proliferation. Schwann cell activation-related markers including p-Erk1/2 and p75 were upregulated after LESW treatment. Conclusion LESW-induced endogenous progenitor cell recruitment and Schwann cell activation coincides with angiogenesis, tissue, and nerve generation in a rat model of pelvic neurovascular injuries.
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AB48. Low-Energy Shock wave therapy and its application to erectile dysfunction
Translational Andrology and Urology, 2014Co-Authors: Zhongcheng XinAbstract:Although phosphodiesterase type 5 inhibitors (PDE5Is) are a revolution in the treatment of erectile dysfunction (ED) and have been marketed since 1998, which could not restore pathological changes in the penis. Low-Energy Shock wave therapy (LESWT) has been developed for treating ED, and clinical studies have shown that LESWT has the potential to affect PDE5I non-responders with ED with few adverse effects. Animal studies have shown that LESWT significantly improves penile hemodynamics and restores pathological changes in penis of diabetic ED animal models. Although the mechanisms remain to be further investigated, recent studies have reported that LESWT could partially restore corpus cavernosum fibromuscular pathological changes, endothelial dysfunction, and peripheral neuropathy. LESWT could be a novel modality for treating ED, and particularly PDE5I non-responders with organic ED, in the near future. However, further extensive evidence-based basic and clinical studies are needed.
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evaluation of the effect of different doses of low Energy Shock wave therapy on the erectile function of streptozotocin stz induced diabetic rats
International Journal of Molecular Sciences, 2013Co-Authors: Jing Liu, Lin Wang, Feng Zhou, Guangyi Bai, Ruili Guan, Zezhu Gao, Wenjie Tian, Zhongcheng XinAbstract:To investigate the therapeutic effect of different doses of low Energy Shock wave therapy (LESWT) on the erectile dysfunction (ED) in streptozotocin (STZ) induced diabetic rats. SD rats (n = 75) were randomly divided into 5 groups (normal control, diabetic control, 3 different dose LESWT treated diabetic groups). Diabetic rats were induced by intra-peritoneal injection of STZ (60 mg/kg) and rats with fasting blood glucose ≥ 300 mg/dL were selected as diabetic models. Twelve weeks later, different doses of LESWT (100, 200 and 300 Shocks each time) treatment on penises were used to treat ED (7.33 MPa, 2 Shocks/s) three times a week for two weeks. The erectile function was evaluated by intracavernous pressure (ICP) after 1 week washout period. Then the penises were harvested for histological study. The results showed LESWT could significantly improve the erectile function of diabetic rats, increase smooth muscle and endothelial contents, up-regulate the expression of α-SMA, vWF, nNOS and VEGF, and down- regulate the expression of RAGE in corpus cavernosum. The therapeutic effect might relate to treatment dose positively, and the maximal therapeutic effect was noted in the LESWT300 group. Consequently, 300 Shocks each time might be the ideal LESWT dose for diabetic ED treatment.
Jack A. Schalken - One of the best experts on this subject based on the ideXlab platform.
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The influence of high-Energy Shock waves on the development of metastases
Ultrasound in medicine & biology, 1996Co-Authors: G.o.n. Oosterhof, Erik B. Cornel, G.a.h.j. Smits, Frans M.j. Debruyne, Jack A. SchalkenAbstract:Abstract The hypothesis that exposure of a solid tumor to high-Energy Shock waves (HESW) could lead to an increase of metastases was investigated in an animal model. The highly metastatic AT-6 Dunning R3327 rat prostate cancer subline was implanted in the hind limb of a Fisher-Copenhagen rat and was exposed to 6000 Shock waves delivered by an experimental lithotripter, or sham-treated, as soon as the tumor had reached a volume of 175–225 mm3. The tumor-bearing leg was amputated 24 h later and the number of metastases was examined 12 weeks thereafter at autopsy. Metastases were seen in 82% of the animals exposed to HESW and in 25% of the sham-treated animals. There was no significant difference in weight of the lungs that contained metastases, between sham and treated animals. These results were confirmed in a second experiment. We conclude that the metastatic spread of tumors with a high metastatic potential may be enhanced by Shock-wave exposure.
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THE EFFECTS OF SUCCESSIVE HIGH-Energy Shock-WAVE TUMOR ADMINISTRATION ON TUMOR BLOOD FLOW
Ultrasound in medicine & biology, 1995Co-Authors: Erik B. Cornel, G.a.h.j. Smits, Frans M.j. Debruyne, Arend Heerschap, Jack A. Schalken, G.o.n. OosterhofAbstract:The effects of repeated high-Energy Shock wave (HESW) tumor administration on tumor blood flow (TBF) were studied in NU-1 human kidney cancer xenografts. Deuteriated water was used as a magnetic resonance spectroscopic detectable tracer for measuring tumor blood flow. Tumors were exposed twice to 800 electromagnetically generated HESW, with a 24-h interval or sham exposed. No changes in TBF occurred after sham exposure to HESW. TBF levels 2 h after the first and second HESW application were, respectively, 46% and 37% lower than the mean preexposure TBF value and returned to normal levels within 16 h. There was statistically no difference found between the effects on tumor blood flow after the first and second HESW exposure. These observations are in agreement with earlier studies and provide a rationale to shorten the time interval between HESW monotreatments to 2 to 3 h.
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High Energy Shock Waves Induced Increase in the Local Concentration of Systemically Given TNF-α
The Journal of Urology, 1994Co-Authors: Erik B. Cornel, Frans M.j. Debruyne, Jack A. Schalken, Egbert Oosterwijk, J.d. Van De Streek, G. Grutters, G.o.n. OosterhofAbstract:The influence of high Energy Shock waves (HESW) on the local concentration of systemically given TNF-alpha was studied in a syngeneic rat bladder tumor model. 125I-TNF-alpha was injected intravenously and within 1 minute was followed by HESW tumor exposure. High Energy Shock waves were generated using an experimental Shock wave emitter based on the Siemens Lithostar Plus. Administration of HESW to RBT323 tumors resulted in a 3-fold increase in uptake of 125I-TNF-alpha in the tumor compared with nontreated tumors, whereas the tissue distribution and pharmacokinetics of nontumor tissue were similar irrespective of HESW exposure. These results demonstrate that HESW administration leads to an increase in the local concentration of a systemically given drug. The capacity of HESW to increase local drug concentrations will be of importance in designing more effective combined treatment strategies.
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High Energy Shock waves induced increase in the local concentration of systemically given TNF-alpha.
The Journal of urology, 1994Co-Authors: E B Cornel, Jack A. Schalken, Egbert Oosterwijk, J.d. Van De Streek, G. Grutters, F M Debruyne, G.o.n. OosterhofAbstract:The influence of high Energy Shock waves (HESW) on the local concentration of systemically given TNF-alpha was studied in a syngeneic rat bladder tumor model. 125I-TNF-alpha was injected intravenously and within 1 minute was followed by HESW tumor exposure. High Energy Shock waves were generated using an experimental Shock wave emitter based on the Siemens Lithostar Plus. Administration of HESW to RBT323 tumors resulted in a 3-fold increase in uptake of 125I-TNF-alpha in the tumor compared with nontreated tumors, whereas the tissue distribution and pharmacokinetics of nontumor tissue were similar irrespective of HESW exposure. These results demonstrate that HESW administration leads to an increase in the local concentration of a systemically given drug. The capacity of HESW to increase local drug concentrations will be of importance in designing more effective combined treatment strategies.
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Magnetic Resonance Spectroscopic Evaluation of the Effects of High-Energy Shock Waves Administered to a Human Kidney Cancer Xenograft
Magnetic Resonance in Nephrourology, 1993Co-Authors: Arend Heerschap, G.a.h.j. Smits, G.o.n. Oosterhof, H. J. Van Den Boogert, F. M. J. Debruyne, J. H. J. Ruijs, Jack A. SchalkenAbstract:The administration of high-Energy Shock waves (HESW) in clinical lithotripsy for kidney stone fragmentation is well known [1], but recent studies have revealed other potential biomedical applications for this type of acoustic Energy. It has been known for a few years that HESW can be used to suppress tumor growth in vivo [2]; a temporary suppression of tumor growth for different xenografts after focal administration of HESW has been demonstrated [3]. However, the processes underlying this biological effect are poorly understood. HESW may affect tumor cells and their metabolism both directly and indirectly (for example, at the vascular level).
Lin Wang - One of the best experts on this subject based on the ideXlab platform.
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low Energy Shock wave therapy ameliorates erectile dysfunction in a pelvic neurovascular injuries rat model
The Journal of Sexual Medicine, 2016Co-Authors: Melanie P Matheu, Fionna Sun, Lin Wang, Melissa T Sanford, Hongxiu Ning, Lia Banie, Yungchin Lee, Zhongcheng XinAbstract:Abstract Introduction Erectile dysfunction (ED) caused by pelvic injuries is a common complication of civil and battlefield trauma with multiple neurovascular factors involved, and no effective therapeutic approach is available. Aims To test the effect and mechanisms of low-Energy Shock wave (LESW) therapy in a rat ED model induced by pelvic neurovascular injuries. Methods Thirty-two male Sprague-Dawley rats injected with 5-ethynyl-2′-deoxyuridine (EdU) at newborn were divided into 4 groups: sham surgery (Sham), pelvic neurovascular injury by bilateral cavernous nerve injury and internal pudendal bundle injury (PVNI), PVNI treated with LESW at low Energy (Low), and PVNI treated with LESW at high Energy (High). After LESW treatment, rats underwent erectile function measurement and the tissues were harvested for histologic and molecular study. To examine the effect of LESW on Schwann cells, in vitro studies were conducted. Main Outcome Measurements The intracavernous pressure (ICP) measurement, histological examination, and Western blot (WB) were conducted. Cell cycle, Schwann cell activation-related markers were examined in in vitro experiments. Results LESW treatment improves erectile function in a rat model of pelvic neurovascular injury by leading to angiogenesis, tissue restoration, and nerve generation with more endogenous EdU + progenitor cells recruited to the damaged area and activation of Schwann cells. LESW facilitates more complete re-innervation of penile tissue with regeneration of neuronal nitric oxide synthase (nNOS)-positive nerves from the MPG to the penis. In vitro experiments demonstrated that LESW has a direct effect on Schwann cell proliferation. Schwann cell activation-related markers including p-Erk1/2 and p75 were upregulated after LESW treatment. Conclusion LESW-induced endogenous progenitor cell recruitment and Schwann cell activation coincides with angiogenesis, tissue, and nerve generation in a rat model of pelvic neurovascular injuries.
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evaluation of the effect of different doses of low Energy Shock wave therapy on the erectile function of streptozotocin stz induced diabetic rats
International Journal of Molecular Sciences, 2013Co-Authors: Jing Liu, Lin Wang, Feng Zhou, Guangyi Bai, Ruili Guan, Zezhu Gao, Wenjie Tian, Zhongcheng XinAbstract:To investigate the therapeutic effect of different doses of low Energy Shock wave therapy (LESWT) on the erectile dysfunction (ED) in streptozotocin (STZ) induced diabetic rats. SD rats (n = 75) were randomly divided into 5 groups (normal control, diabetic control, 3 different dose LESWT treated diabetic groups). Diabetic rats were induced by intra-peritoneal injection of STZ (60 mg/kg) and rats with fasting blood glucose ≥ 300 mg/dL were selected as diabetic models. Twelve weeks later, different doses of LESWT (100, 200 and 300 Shocks each time) treatment on penises were used to treat ED (7.33 MPa, 2 Shocks/s) three times a week for two weeks. The erectile function was evaluated by intracavernous pressure (ICP) after 1 week washout period. Then the penises were harvested for histological study. The results showed LESWT could significantly improve the erectile function of diabetic rats, increase smooth muscle and endothelial contents, up-regulate the expression of α-SMA, vWF, nNOS and VEGF, and down- regulate the expression of RAGE in corpus cavernosum. The therapeutic effect might relate to treatment dose positively, and the maximal therapeutic effect was noted in the LESWT300 group. Consequently, 300 Shocks each time might be the ideal LESWT dose for diabetic ED treatment.
Joao Paulo Zambon - One of the best experts on this subject based on the ideXlab platform.
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Editorial Comment on “Low Energy Shock Wave Therapy Ameliorates Erectile Dysfunction in a Pelvic Neurovascular Injuries Rat Model”
The journal of sexual medicine, 2016Co-Authors: Joao Paulo ZambonAbstract:The authors present a novel application of Shock wave therapy in rats with erectile dysfunction (ED). A rat model of ED was developed by bilateral cavernous nerve and internal pudendal bundle injuries. Forty-eight hours after nerve damage, animals were randomly treated with a low-Energy Shock wave (0.06 mJ/mm, 300 pulses at 3 Hz) or a high-Energy Shock wave (0.09 mJ/mm, 1,000 pulses at 3 Hz). Functional and histologic assessments after the intervention showed improvements in intracavernosal pressure, increased vascularization, and nerve regeneration.