The Experts below are selected from a list of 1020 Experts worldwide ranked by ideXlab platform
Ying Wang - One of the best experts on this subject based on the ideXlab platform.
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synthesis of icariin in tobacco leaf by overexpression of a glucosyltransferase gene from Epimedium sagittatum
Industrial Crops and Products, 2020Co-Authors: Xiaoman Yang, Wenjun Huang, Yanjun Zhang, Jianjun Chen, Xing Yan, Zhihua Zhou, Ying WangAbstract:Abstract Epimedium plants are globally known for gardening and medicinal purposes. They are a potential resource of biologically active pharmaceutical compounds that can be used to treat various diseases. Among these compounds, icariin, a glycosylated flavonoid, has significant anti-tumor, bone growth, and neuroprotective effects; however, its biosynthetic pathway remains unknown. In this study, icariin accumulation during the development of leaves of Epimedium sagittatum was investigated. We identified a glycosyltransferase (Epimedium sagittatum glycosyltransferase, EsGT1) gene from this species using transcriptional analysis. Heterogeneously expressed EsGT1 protein transferred icariside II to icariin at the 7-OH site and its catalytic function was validated in vivo by the overexpression of the gene. In the recombinant protein assay, EsGT1 was also active in the glycosylation of kaempferol, an intermediate product of icariin. These results characterized the final step of glycosyltransferase in icariin synthesis and suggested an economical and efficient alternative source of icariin using a tobacco biotechnological method.
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the complete chloroplast genome of Epimedium pubescens maxim berberidaceae a traditional chinese medicine herb
Mitochondrial DNA Part B, 2020Co-Authors: Ruoqi Huang, Ying Wang, Qiong Liang, Taejin Yang, Yanjun ZhangAbstract:Epimedium brevicornu Maxim. is a traditional Chinese medicine herb with good effects on many diseases. In the present paper, the complete chloroplast genome of E. brevicornu was sequenced. The comp...
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Functional Characterization of a Novel R2R3-MYB Transcription Factor Modulating the Flavonoid Biosynthetic Pathway from Epimedium sagittatum
Frontiers Media S.A., 2017Co-Authors: Wenjun Huang, Ying WangAbstract:Epimedium species have been widely used both as traditional Chinese medicinal plants and ornamental perennials. Both flavonols, acting as the major bioactive components (BCs) and anthocyanins, predominantly contributing to the color diversity of Epimedium flowers belong to different classes of flavonoids. It is well-acknowledged that flavonoid biosynthetic pathway is predominantly regulated by R2R3-MYB transcription factor (TF) as well as bHLH TF and WD40 protein at the transcriptional level. MYB TFs specifically regulating anthocyanin or flavonol biosynthetic pathway have been already isolated and functionally characterized from Epimedium sagittatum, but a R2R3-MYB TF involved in regulating both these two pathways has not been functionally characterized to date in Epimedium plants. In this study, we report the functional characterization of EsMYB9, a R2R3-MYB TF previously isolated from E. sagittatum. The previous study indicated that EsMYB9 belongs to a small subfamily of R2R3-MYB TFs containing grape VvMYB5a and VvMYB5b TFs, which regulate flavonoid biosynthetic pathway. The present studies show that overexpression of EsMYB9 in tobacco leads to increased transcript levels of flavonoid pathway genes and increased contents of anthocyanins and flavonols. Yeast two-hybrid assay indicates that the C-terminal region of EsMYB9 contributes to the autoactivation activity, and EsMYB9 interacts with EsTT8 or AtTT8 bHLH regulator. Transient reporter assay shows that EsMYB9 slightly activates the expression of EsCHS (chalcone synthase) promoter in transiently transformed leaves of Nicotiana benthamiana, but the addition of AtTT8 or EsTT8 bHLH regulator strongly enhances the transcriptional activation of EsMYB9 against five promoters of the flavonoid pathway genes except EsFLS (flavonol synthase). In addition, co-transformation of EsMYB9 and EsTT8 in transiently transfected tobacco leaves strongly induces the expressions of flavonoid biosynthetic genes. The potential role of EsMYB9 in modulating the biosynthesis and accumulation of sucrose-induced anthocyanin and flavonol-derived BCs is also discussed. These findings suggest that EsMYB9 is a novel R2R3-MYB TF, which regulates the flavonoid biosynthetic pathway in Epimedium, but distinctly different with the anthocyanin or flavonol-specific MYB regulators identified previously in Epimedium plants
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Five new synonyms in Epimedium (Berberidaceae) from China 1 Five new synonyms in Epimedium (Berberidaceae) from China
2016Co-Authors: Yanjun Zhang, Haishan Dang, Ying WangAbstract:Five new synonyms in Chinese Epimedium are designated in the present paper. Epimedium chlorandrum is treated as a synonym of E. acuminatum; Epimedium rhizomatosum as a synonym of E. membranaceum; Epimedium brachyrrhizum as a synonym of E. leptorrhizum; Epimedium dewuense as a synonym of E. dolichostemon; and Epimedium sagittatum var. oblongifoliolatum as a synonym of E. borealiguizhouense
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A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway
2016Co-Authors: Wenjun Huang, Shaohua Zeng, Wei Sun, Ming Luo, Sitakanta Pattanaik, Ling Yuan, Ying WangAbstract:Herba epimedii (Epimedium), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. Epimedium species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in Epimedium. Here, we reported the isolation and functional characterization of the first R2R3-MYB TF (EsMYBA1) from Epimedium sagittatum (Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes Arabidopsis AtPAP1, apple MdMYB10 and legume MtLAP1. EsMYBA1 was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of EsMYBA1 resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS). In both transgenic tobacco and Arabidopsis, overexpression of EsMYBA1 induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes
Shaohua Zeng - One of the best experts on this subject based on the ideXlab platform.
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leaf like sepals induced by ectopic expression of a short vegetative phase svp like mads box gene from the basal eudicot Epimedium sagittatum
Frontiers in Plant Science, 2016Co-Authors: Shaohua Zeng, Erik SouerAbstract:Epimedium L. (Berberidaceae, Ranales), a perennial traditional Chinese medicinal herb, has become a new popular landscape plant for ground cover and pot culture in many countries based on its excellent ornamental characteristics and, distinctive and diverse floral morphology. However, little is known about the molecular genetics of flower development in Epimedium sagittatum. Here, we describe the characterization of EsSVP that encodes a protein sharing 68, 54, and 35% similarity with SVP, AGAMOUS-like 24 (AGL24) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) in Arabidopsis, respectively. Quantitative RT-PCR (qRT-PCR) indicated that EsSVP transcripts were principally found in petiole and leaf tissues, with little expression in roots and flowers and no in fruits. The highest EsSVP expression was observed in leaves. The flowering time of 35S::EsSVP in most Arabidopsis thaliana and in all petunia plants was not affected in both photoperiod conditions, but 35S::EsSVP 5# and 35S::EsSVP 1# Arabidopsis lines induced late and early flowering under long day (LD, 14 h light/10 h dark) and short day (SD, 10 h light/14 h dark) conditions, respectively. The 35S::EsSVP Arabidopsis produced extra secondary inflorescence or floral meristems in the axils of the leaf-like sepals with excrescent trichomes, and leaf-like sepals not able to enclose the inner three whorls completely. Moreover, almost all transgenic Arabidopsis plants showed persistent sepals around the completely matured fruits. Upon ectopic expression of 35S::EsSVP in Petunia W115, sepals were enlarged, sometimes to the size of leaves; corollas were greenish and did not fully open. These results suggest that EsSVP is involved in inflorescence meristem identity and flowering time regulation in some conditions. Although, the SVP homologs might have suffered functional diversification among diverse species between core and basal eudicots, the protein functions are conserved between Arabidopsis/Petunia and Epimedium.
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A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway
2016Co-Authors: Wenjun Huang, Shaohua Zeng, Wei Sun, Ming Luo, Sitakanta Pattanaik, Ling Yuan, Ying WangAbstract:Herba epimedii (Epimedium), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. Epimedium species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in Epimedium. Here, we reported the isolation and functional characterization of the first R2R3-MYB TF (EsMYBA1) from Epimedium sagittatum (Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes Arabidopsis AtPAP1, apple MdMYB10 and legume MtLAP1. EsMYBA1 was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of EsMYBA1 resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS). In both transgenic tobacco and Arabidopsis, overexpression of EsMYBA1 induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes
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Exploration of genetic diversity among medicinally important genus Epimedium species based on genomic and EST-SSR marker
2015Co-Authors: Zubaida Yousaf, Shaohua Zeng, Yanjun Zhang, Ying WangAbstract:Epimedium species has gained prime importance due to their medicinal and economic values. Therefore, in this study, 26 genomic SSR and 10 EST-SSR markers were developed for 13 medicinal species of the Epimedium genus and one out-group species Vancouveria hexandra W. J. Hooker to explore the existing genetic diversity. A total of 100 alleles by genomic SSR and 65 by EST-SSR were detected. The genomic SSR markers were presented between 2–7 alleles per locus. The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.00 to 4.5 and 0.0254 to 2.8108, respectively. Similarly, for EST-SSR, these values were ranged from 3.00 to 4.00 and 1.9650 to 2.7142. The number of alleles for EST-SSR markers ranged from 3 to 10 with an average of 3.51 per loci. It has been concluded that medicinally important species of the genus Epimedium possesses lower intraspecific genetic variation.
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Isolation and molecular characterization of thirteen R2R3-MYB transcription factors from Epimedium sagittatum
2013Co-Authors: Wenjun Huang, Shaohua Zeng, Wei Sun, Gong Xiao, Ying WangAbstract:medicinal plant, has been widely used for treating sexual dysfunction and osteoporosis in China. The main bioactive components in herba epimedii are prenylated flavonol glycosides, which are end products of a branch of the flavonoid biosynthetic pathway. The MYB transcription factors (TF) act as activators or repressors to regulate the flavonoid pathway. In this study, 13 full-length cDNA clones of R2R3-MYB TFs from E. sagittatum (designated as EsMYB1 to EsMYB13) were isolated and characterized. Sequence similarity and phylogenetic analysis placed nine R2R3-MYB members of E. sagittatum into five subgroups of the Arabidopsis R2R3-MYB family, while four members were not clustered into a defined subgroup. The number and length of introns from Epimedium R2R3-MYB genes varied significantly, but intron positions and phases were well conserved. Expression patterns of Epimedium R2R3-MYB genes in various tissues showed diverse. Finally, it is suggested that five Epimedium R2R3-MYB genes may be involved in regulating the flavonoid pathway and could be used as valuable candidate genes for metaboli
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cloning and characterization of phenylalanine ammonia lyase in medicinal Epimedium species
Plant Cell Tissue and Organ Culture, 2013Co-Authors: Shaohua Zeng, Wenjun Huang, Ying WangAbstract:Phenylalanine ammonia-lyase (PAL) plays an important role in the phenylpropanoid pathway and in accumulation of major secondary metabolites in medicinal Epimedium species, including icariin, epimedin A, epimedin B, and epimedin C (hereafter designated as active components). In this study, three Epimedium sagittatum PALs (EsPALs) mRNA sequences, designated respectively as EsPAL1, EsPAL2 and EsPAL3 deduced to encode 708, 716, and 739 amino acids, were isolated and characterized. Based on sequence and phylogenetic analyses, EsPAL1 was found to be closer to EsPAL2 than to EsPAL3. Spatio-temporal expression profiles and metabolic accumulation profiles revealed that EsPAL3 was highly expressed in flavonoid-enriched tissues and leaves at certain developmental stages along with high levels of active components, while EsPAL1 was highly expressed in leathery leaves along with high lignin content. Under light stress, the total flavonoid content was enhanced by 100 μM phytohormones tested or 5 % sucrose through upregulating different EsPAL isoform(s). Our findings have laid a solid foundation for improving the content of bioactive components in Epimedium via metabolically engineering EsPAL.
Yanjun Zhang - One of the best experts on this subject based on the ideXlab platform.
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synthesis of icariin in tobacco leaf by overexpression of a glucosyltransferase gene from Epimedium sagittatum
Industrial Crops and Products, 2020Co-Authors: Xiaoman Yang, Wenjun Huang, Yanjun Zhang, Jianjun Chen, Xing Yan, Zhihua Zhou, Ying WangAbstract:Abstract Epimedium plants are globally known for gardening and medicinal purposes. They are a potential resource of biologically active pharmaceutical compounds that can be used to treat various diseases. Among these compounds, icariin, a glycosylated flavonoid, has significant anti-tumor, bone growth, and neuroprotective effects; however, its biosynthetic pathway remains unknown. In this study, icariin accumulation during the development of leaves of Epimedium sagittatum was investigated. We identified a glycosyltransferase (Epimedium sagittatum glycosyltransferase, EsGT1) gene from this species using transcriptional analysis. Heterogeneously expressed EsGT1 protein transferred icariside II to icariin at the 7-OH site and its catalytic function was validated in vivo by the overexpression of the gene. In the recombinant protein assay, EsGT1 was also active in the glycosylation of kaempferol, an intermediate product of icariin. These results characterized the final step of glycosyltransferase in icariin synthesis and suggested an economical and efficient alternative source of icariin using a tobacco biotechnological method.
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the complete chloroplast genome of Epimedium pubescens maxim berberidaceae a traditional chinese medicine herb
Mitochondrial DNA Part B, 2020Co-Authors: Ruoqi Huang, Ying Wang, Qiong Liang, Taejin Yang, Yanjun ZhangAbstract:Epimedium brevicornu Maxim. is a traditional Chinese medicine herb with good effects on many diseases. In the present paper, the complete chloroplast genome of E. brevicornu was sequenced. The comp...
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Five new synonyms in Epimedium (Berberidaceae) from China 1 Five new synonyms in Epimedium (Berberidaceae) from China
2016Co-Authors: Yanjun Zhang, Haishan Dang, Ying WangAbstract:Five new synonyms in Chinese Epimedium are designated in the present paper. Epimedium chlorandrum is treated as a synonym of E. acuminatum; Epimedium rhizomatosum as a synonym of E. membranaceum; Epimedium brachyrrhizum as a synonym of E. leptorrhizum; Epimedium dewuense as a synonym of E. dolichostemon; and Epimedium sagittatum var. oblongifoliolatum as a synonym of E. borealiguizhouense
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the complete chloroplast genome sequences of five Epimedium species lights into phylogenetic and taxonomic analyses
Frontiers in Plant Science, 2016Co-Authors: Yanjun Zhang, Taejin Yang, Jianjun Chen, Liuwen Du, Li Wu, Weiming Hu, Wei Zhang, Ying WangAbstract:Epimedium L. is a phylogenetically and economically important genus in the family Berberidaceae. We here sequenced the complete chloroplast (cp) genomes of four Epimedium species using Illumina sequencing technology via a combination of de novo and reference-guided assembly, which was also the first comprehensive cp genome analysis on Epimedium combining the cp genome sequence of E. koreanum previously reported. The five Epimedium cp genomes exhibited typical quadripartite and circular structure that was rather conserved in genomic structure and the synteny of gene order. However, these cp genomes presented obvious variations at the boundaries of the four regions because of the expansion and contraction of the inverted repeat (IR) region and the single-copy (SC) boundary regions. The trnQ-UUG duplication occurred in the five Epimedium cp genomes, which was not found in the other basal eudicotyledons. The rapidly evolving cp genome regions were detected among the five cp genomes, as well as the difference of simple sequence repeats (SSR) and repeat sequence were identified. Phylogenetic relationships among the five Epimedium species based on their cp genomes showed accordance with the updated system of the genus on the whole, but reminded that the evolutionary relationships and the divisions of the genus need further investigation applying more evidences. The availability of these cp genomes provided valuable genetic information for accurately identifying species, taxonomy and phylogenetic resolution and evolution of Epimedium, and assist in exploration and utilization of Epimedium plants.
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five new synonyms in Epimedium berberidaceae from china
PhytoKeys, 2015Co-Authors: Yanjun Zhang, Haishan Dang, Ying WangAbstract:Five new synonyms in Chinese Epimedium are designated in the present paper. Epimedium chlorandrum is treated as a synonym of Epimedium acuminatum; Epimedium rhizomatosum as a synonym of Epimedium membranaceum; Epimedium brachyrrhizum as a synonym of Epimedium leptorrhizum; Epimedium dewuense as a synonym of Epimedium dolichostemon; and Epimedium sagittatum var. oblongifoliolatum as a synonym of Epimedium borealiguizhouense.
Wenjun Huang - One of the best experts on this subject based on the ideXlab platform.
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synthesis of icariin in tobacco leaf by overexpression of a glucosyltransferase gene from Epimedium sagittatum
Industrial Crops and Products, 2020Co-Authors: Xiaoman Yang, Wenjun Huang, Yanjun Zhang, Jianjun Chen, Xing Yan, Zhihua Zhou, Ying WangAbstract:Abstract Epimedium plants are globally known for gardening and medicinal purposes. They are a potential resource of biologically active pharmaceutical compounds that can be used to treat various diseases. Among these compounds, icariin, a glycosylated flavonoid, has significant anti-tumor, bone growth, and neuroprotective effects; however, its biosynthetic pathway remains unknown. In this study, icariin accumulation during the development of leaves of Epimedium sagittatum was investigated. We identified a glycosyltransferase (Epimedium sagittatum glycosyltransferase, EsGT1) gene from this species using transcriptional analysis. Heterogeneously expressed EsGT1 protein transferred icariside II to icariin at the 7-OH site and its catalytic function was validated in vivo by the overexpression of the gene. In the recombinant protein assay, EsGT1 was also active in the glycosylation of kaempferol, an intermediate product of icariin. These results characterized the final step of glycosyltransferase in icariin synthesis and suggested an economical and efficient alternative source of icariin using a tobacco biotechnological method.
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Functional Characterization of a Novel R2R3-MYB Transcription Factor Modulating the Flavonoid Biosynthetic Pathway from Epimedium sagittatum
Frontiers Media S.A., 2017Co-Authors: Wenjun Huang, Ying WangAbstract:Epimedium species have been widely used both as traditional Chinese medicinal plants and ornamental perennials. Both flavonols, acting as the major bioactive components (BCs) and anthocyanins, predominantly contributing to the color diversity of Epimedium flowers belong to different classes of flavonoids. It is well-acknowledged that flavonoid biosynthetic pathway is predominantly regulated by R2R3-MYB transcription factor (TF) as well as bHLH TF and WD40 protein at the transcriptional level. MYB TFs specifically regulating anthocyanin or flavonol biosynthetic pathway have been already isolated and functionally characterized from Epimedium sagittatum, but a R2R3-MYB TF involved in regulating both these two pathways has not been functionally characterized to date in Epimedium plants. In this study, we report the functional characterization of EsMYB9, a R2R3-MYB TF previously isolated from E. sagittatum. The previous study indicated that EsMYB9 belongs to a small subfamily of R2R3-MYB TFs containing grape VvMYB5a and VvMYB5b TFs, which regulate flavonoid biosynthetic pathway. The present studies show that overexpression of EsMYB9 in tobacco leads to increased transcript levels of flavonoid pathway genes and increased contents of anthocyanins and flavonols. Yeast two-hybrid assay indicates that the C-terminal region of EsMYB9 contributes to the autoactivation activity, and EsMYB9 interacts with EsTT8 or AtTT8 bHLH regulator. Transient reporter assay shows that EsMYB9 slightly activates the expression of EsCHS (chalcone synthase) promoter in transiently transformed leaves of Nicotiana benthamiana, but the addition of AtTT8 or EsTT8 bHLH regulator strongly enhances the transcriptional activation of EsMYB9 against five promoters of the flavonoid pathway genes except EsFLS (flavonol synthase). In addition, co-transformation of EsMYB9 and EsTT8 in transiently transfected tobacco leaves strongly induces the expressions of flavonoid biosynthetic genes. The potential role of EsMYB9 in modulating the biosynthesis and accumulation of sucrose-induced anthocyanin and flavonol-derived BCs is also discussed. These findings suggest that EsMYB9 is a novel R2R3-MYB TF, which regulates the flavonoid biosynthetic pathway in Epimedium, but distinctly different with the anthocyanin or flavonol-specific MYB regulators identified previously in Epimedium plants
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A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway
2016Co-Authors: Wenjun Huang, Shaohua Zeng, Wei Sun, Ming Luo, Sitakanta Pattanaik, Ling Yuan, Ying WangAbstract:Herba epimedii (Epimedium), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. Epimedium species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in Epimedium. Here, we reported the isolation and functional characterization of the first R2R3-MYB TF (EsMYBA1) from Epimedium sagittatum (Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes Arabidopsis AtPAP1, apple MdMYB10 and legume MtLAP1. EsMYBA1 was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of EsMYBA1 resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS). In both transgenic tobacco and Arabidopsis, overexpression of EsMYBA1 induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes
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a r2r3 myb transcription factor regulates the flavonol biosynthetic pathway in a traditional chinese medicinal plant Epimedium sagittatum
Frontiers in Plant Science, 2016Co-Authors: Wenjun Huang, Jianjun Chen, Ling Yuan, A B M Khaldun, Chanjuan Zhang, Ying WangAbstract:Flavonols as plant secondary metabolites with vital roles in plant development and defense against UV light, have been demonstrated to be the main bioactive components (BCs) in the genus Epimedium plants, several species of which are used as materials for Herba Epimedii, an important traditional Chinese medicine. The flavonol biosynthetic pathway genes had been already isolated from Epimedium sagittatum, but a R2R3-MYB transcription factor regulating the flavonol synthesis has not been functionally characterized so far in Epimedium plants. In this study, we isolated and characterized the R2R3-MYB transcription factor EsMYBF1 involved in regulation of the flavonol biosynthetic pathway from E. sagittatum. Sequence analysis indicated that EsMYBF1 belongs to the subgroup 7 of R2R3-MYB family which contains the flavonol-specific MYB regulators identified to date. Transient reporter assay showed that EsMYBF1 strongly activated the promoters of EsF3H (flavanone 3-hydroxylase) and EsFLS (flavonol synthase), but not the promoters of EsDFRs (dihydroflavonol 4-reductase) and EsANS (anthocyanidin synthase) in transiently transformed Nicotiana benthamiana leaves. Both yeast two-hybrid assay and transient reporter assay validated EsMYBF1 to be independent of EsTT8, or AtTT8 bHLH regulators of the flavonoid pathway as cofactors. Ectopic expression of EsMYBF1 in transgenic tobacco resulted in the increased flavonol content and the decreased anthocyanin content in flowers. Correspondingly, the structural genes involved in flavonol synthesis were upregulated in the EsMYBF1 overexpression lines, including NtCHS (chalcone synthase), NtCHI (chalcone isomerase), NtF3H and NtFLS, whereas the late biosynthetic genes of the anthocyanin pathway (NtDFR and NtANS) were remarkably downregulated, compared to the controls. These results suggest that EsMYBF1 is a flavonol-specific R2R3-MYB regulator, and involved in regulation of the biosynthesis of the flavonol-derived BCs in E. sagittatum. Thus, identification and functional characterization of EsMYBF1 provide insight into understanding the biosynthesis and regulation of the flavonol-derived BCs in Epimedium plants, and also provide an effective tool gene for genetic manipulation to improve the flavonol synthesis.
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A R2R3-MYB transcription factor regulates the flavonol biosynthetic pathway in a traditional Chinese medicinal plant, Epimedium sagittatum
Frontiers Media S.A., 2016Co-Authors: Wenjun Huang, Jianjun Chen, Ling Yuan, A B M Khaldun, Chanjuan Zhang, Ying WangAbstract:Flavonols as plant secondary metabolites with vital roles in plant development and defense against UV light, have been demonstrated to be the main bioactive components in the genus Epimedium plants, several species of which are used as materials for Herba Epimedii, an important traditional Chinese medicine. The flavonol biosynthetic pathway genes had been already isolated from E. sagittatum, but a R2R3-MYB transcription factor regulating the flavonol synthesis has not been functionally characterized so far in Epimedium plants. In this study, we isolated and characterized the R2R3-MYB transcription factor EsMYBF1 involved in regulation of the flavonol biosynthetic pathway from E. sagittatum. Sequence analysis indicated that EsMYBF1 belongs to the subgroup 7 of R2R3-MYB family which contains the flavonol-specific MYB regulators identified to date. Transient reporter assay showed that EsMYBF1 strongly activated the promoters of EsF3H (flavanone 3-hydroxylase) and EsFLS (flavonol synthase), but not the promoters of EsDFRs (dihydroflavonol 4-reductase) and EsANS (anthocyanidin synthase) in transiently transformed Nicotiana benthamiana leaves. Both yeast two-hybrid assay and transient reporter assay validated EsMYBF1 to be independent of EsTT8, or AtTT8 bHLH regulators of the flavonoid pathway as cofactors. Ectopic expression of EsMYBF1 in transgenic tobacco resulted in the increased flavonol content and the decreased anthocyanin content in flowers. Correspondingly, the structural genes involved in flavonol synthesis were upregulated in the EsMYBF1 overexpression lines, including NtCHS (chalcone synthase), NtCHI (chalcone isomerase), NtF3H and NtFLS, whereas the late biosynthetic genes of the anthocyanin pathway (NtDFR and NtANS) were remarkably downregulated, compared to the controls. These results suggest that EsMYBF1 is a flavonol-specific R2R3-MYB regulator, and involved in regulation of the biosynthesis of the flavonol-derived bioactive components in E. sagittatum. Thus, identification and functional characterization of EsMYBF1 provide insight into understanding the biosynthesis and regulation of the flavonol-derived bioactive components in Epimedium plants, and also provide an effective tool gene for genetic manipulation to improve the flavonol synthesis
Baolin Guo - One of the best experts on this subject based on the ideXlab platform.
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the complete chloroplast genome of Epimedium rhizomatosum berberidaceae an endemic plant species to china
Mitochondrial DNA Part B, 2020Co-Authors: Guoan Shen, Xiang Liu, Yu Yao, Yanjiao Luo, Qianru Yang, Fengmei Suo, Cheng Zhang, Baolin GuoAbstract:Epimedium rhizomatosum is a rare endemic plant species inhabited in some high-elevation mountain area in the Sichuan province of China. In this study, we assembled the complete chloroplast genome o...
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the complete chloroplast genome of Epimedium tianmenshanense berberidaceae
Mitochondrial DNA Part B, 2020Co-Authors: Yue Zhang, Xiang Liu, Baolin Guo, Guoan Shen, Cheng Zhang, Lijuan ZhangAbstract:In this study, the complete chloroplast (cp) genome of Epimedium tianmenshanense was sequenced and assembled. The circular genome is 157,066 bp in size. The whole chloroplast genome of E. tianmenshanense contains 112 unique genes, of which 78 protein-coding genes, 30 tRNA genes, and four rRNA genes. The phylogenetic analysis based on 17 complete chloroplast genomes demonstrated a closer relationship between E. tianmenshanense and E. lishihchenii.
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the complete chloroplast genome of Epimedium mikinorii stearn berberidaceae
Mitochondrial DNA Part B, 2020Co-Authors: Chunmei Wen, Xiang Liu, Yu Yao, Yanjiao Luo, Ting Liu, Qianru Yang, Baolin Guo, Fengmei Suo, Guoan ShenAbstract:Epimedium mikinorii is a vulnerable species in the Epimedium genus of Berberaceae. Here, we sequenced the complete chloroplast genome of E. mikinorii, which is 157,136 bp in length, and is a typica...
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the complete chloroplast genome of Epimedium qingchengshanense g y zhong et b l guo berberidaceae an endangered species endemic to china
Mitochondrial DNA Part B, 2020Co-Authors: Yanjiao Luo, Xiang Liu, Qianru Yang, Baolin Guo, Fengmei Suo, Guoan Shen, Cheng Zhang, Anjia ChenAbstract:Epimedium qingchengshanense G. Y. Zhong & B. L. Guo is an endangered species with high ornamental value and medicinal value in China. In this study, we reported the first complete chloroplast (cp) ...
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Development of Plastid Genomic Resources for Discrimination and Classification of Epimedium wushanense (Berberidaceae).
International journal of molecular sciences, 2019Co-Authors: Guo Mengyue, Baolin Guo, Baosheng Liao, Jingyuan Song, Nitin Mantri, Shilin Chen, Pang XiaohuiAbstract:Epimedium wushanense (Berberidaceae) is recorded as the source plant of Epimedii Wushanensis Folium in the Chinese Pharmacopoeia. However, controversies exist on the classification of E. wushanense and its closely related species, namely, E. pseudowushanense, E. chlorandrum, E. mikinorii, E. ilicifolium, and E. borealiguizhouense. These species are often confused with one another because of their highly similar morphological characteristics. This confusion leads to misuse in the medicinal market threatening efficiency and safety. Here, we studied the plastid genomes of these Epimedium species. Results show that the plastid genomes of E. wushanense and its relative species are typical circular tetramerous structure, with lengths of 156,855–158,251 bp. A total of 112 genes were identified from the Epimedium plastid genomes, including 78 protein-coding, 30 tRNA, and 4 rRNA genes. A loss of rpl32 gene in E. chlorandrum was found for the first time in this study. The phylogenetic trees constructed indicated that E. wushanense can be distinguished from its closely related species. E. wushanense shows a closer relationship to species in ser. Dolichocerae. In conclusion, the use of plastid genomes contributes useful genetic information for identifying medicinally important species E. wushanense and provides new evidence for understanding phylogenetic relationships within the Epimedium genus.