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Eliseu Rodrigues - One of the best experts on this subject based on the ideXlab platform.

  • Natural deep eutectic solvent (NADES)-based blueberry extracts protect against Ethanol-Induced Gastric Ulcer in rats.
    Food research international (Ottawa Ont.), 2020
    Co-Authors: D. T. Da ,silva, M. L. Da ,veiga, M. I. De U. M. Da ,rocha, Renata Fritzsche Rodrigues, Natália Minuzzi Machado, Luana Haselein Maurer, Lauren Fresinghelli Ferreira, Sabrina Somacal, Márcia Vizzotto, Eliseu Rodrigues
    Abstract:

    Blueberry is a polyphenol-rich fruit bearing great bioactive potential. Natural deep eutectic solvents (NADES) emerged as putatively biocompatible solvents that could substitute for toxic organic solvents in the extraction of fruit phenolic compounds for developing nutraceuticals or functional foods. Therefore, the aim of this study was to investigate the gastroprotective effects and the biocompatibility of a blueberry crude extract (CE) obtained using NADES and of the extract fractions (anthocyanin-rich fraction - ARF; non-anthocyanin phenolic fraction - NAPF) in a model of Ethanol-Induced Gastric Ulcer in rats. CE was the NADES-containing, ready-to-use extract that was obtained using choline chloride:glycerol:citric acid NADES (0.5:2:0.5 M ratio). ARF and NAPF were the NADES-free fractions obtained by solid phase purification of CE and were investigated to identify the bioactive fraction responsible for the effects of CE. Animals were treated for 14 days with water, NADES vehicle, CE, ARF, NAPF or lansoprazole (intraGastric) and then received ethanol to induce Gastric Ulcer. CE decreased Ulcer index and preserved the integrity of Gastric mucosa. The pretreatment with CE or ARF reduced glutathione depletion and the inflammatory response. All treatments, including NADES vehicle reduced protein oxidation and nitric oxide overproduction in ethanol-treated rats. Additionally, ARF increased short-chain fatty acids in feces. These findings suggest that NADES can be used to obtain biocompatible extracts of blueberry that exhibit gastroprotective effects with no need of solvent removal. The gastroprotective effects were mainly associated to ARF but NAPF and even NADES vehicle also contributed to some protective effects.

D. T. Da ,silva - One of the best experts on this subject based on the ideXlab platform.

  • Natural deep eutectic solvent (NADES)-based blueberry extracts protect against Ethanol-Induced Gastric Ulcer in rats.
    Food research international (Ottawa Ont.), 2020
    Co-Authors: D. T. Da ,silva, M. L. Da ,veiga, M. I. De U. M. Da ,rocha, Renata Fritzsche Rodrigues, Natália Minuzzi Machado, Luana Haselein Maurer, Lauren Fresinghelli Ferreira, Sabrina Somacal, Márcia Vizzotto, Eliseu Rodrigues
    Abstract:

    Blueberry is a polyphenol-rich fruit bearing great bioactive potential. Natural deep eutectic solvents (NADES) emerged as putatively biocompatible solvents that could substitute for toxic organic solvents in the extraction of fruit phenolic compounds for developing nutraceuticals or functional foods. Therefore, the aim of this study was to investigate the gastroprotective effects and the biocompatibility of a blueberry crude extract (CE) obtained using NADES and of the extract fractions (anthocyanin-rich fraction - ARF; non-anthocyanin phenolic fraction - NAPF) in a model of Ethanol-Induced Gastric Ulcer in rats. CE was the NADES-containing, ready-to-use extract that was obtained using choline chloride:glycerol:citric acid NADES (0.5:2:0.5 M ratio). ARF and NAPF were the NADES-free fractions obtained by solid phase purification of CE and were investigated to identify the bioactive fraction responsible for the effects of CE. Animals were treated for 14 days with water, NADES vehicle, CE, ARF, NAPF or lansoprazole (intraGastric) and then received ethanol to induce Gastric Ulcer. CE decreased Ulcer index and preserved the integrity of Gastric mucosa. The pretreatment with CE or ARF reduced glutathione depletion and the inflammatory response. All treatments, including NADES vehicle reduced protein oxidation and nitric oxide overproduction in ethanol-treated rats. Additionally, ARF increased short-chain fatty acids in feces. These findings suggest that NADES can be used to obtain biocompatible extracts of blueberry that exhibit gastroprotective effects with no need of solvent removal. The gastroprotective effects were mainly associated to ARF but NAPF and even NADES vehicle also contributed to some protective effects.

  • Natural deep eutectic solvent (NADES)-based blueberry extracts protect against Ethanol-Induced Gastric Ulcer in rats.
    'Elsevier BV', 2020
    Co-Authors: D. T. Da ,silva, Rodrigues R. F., Machado N. M., Maurer L. H., Ferreira L. F., Somacal S., M. L. Da ,veiga, M. I. De U. M. Da ,rocha, Vizzotto M., Rodrigues E.
    Abstract:

    Blueberry is a polyphenol-rich fruit bearing great bioactive potential. Natural deep eutectic solvents (NADES) emerged as putatively biocompatible solvents that could substitute for toxic organic solvents in the extraction of fruit phenolic compounds for developing nutraceuticals or functional foods. Therefore, the aim of this study was to investigate the gastroprotective effects and the biocompatibility of a blueberry crude extract (CE) obtained using NADES and of the extract fractions (anthocyanin-rich fraction ? ARF; non-anthocyanin phenolic fraction - NAPF) in a model of Ethanol-Induced Gastric Ulcer in rats. CE was the NADES-containing, ready-to-use extract that was obtained using choline chloride:glycerol:citric acid NADES (0.5:2:0.5 M ratio). ARF and NAPF were the NADES-free fractions obtained by solid phase purification of CE and were investigated to identify the bioactive fraction responsible for the effects of CE. Animals were treated for 14 days with water, NADES vehicle, CE, ARF, NAPF or lansoprazole (intraGastric) and then received ethanol to induce Gastric Ulcer. CE decreased Ulcer index and preserved the integrity of Gastric mucosa. The pretreatment with CE or ARF reduced glutathione depletion and the inflammatory response. All treatments, including NADES vehicle reduced protein oxidation and nitric oxide overproduction in ethanol-treated rats. Additionally, ARF increased short-chain fatty acids in feces. These findings suggest that NADES can be used to obtain biocompatible extracts of blueberry that exhibit gastroprotective effects with no need of solvent removal. The gastroprotective effects were mainly associated to ARF but NAPF and even NADES vehicle also contributed to some protective effects.bitstream/item/218692/1/Natural-deep-eutectic-solvent.pd

Xiaofeng Niu - One of the best experts on this subject based on the ideXlab platform.

  • anti Ulcerogenic effect of cavidine against ethanol induced acute Gastric Ulcer in mice and possible underlying mechanism
    International Immunopharmacology, 2016
    Co-Authors: Xiumei Wang, Yu Wang, Hailin Zhang, Wenbing Zhi, Fang Liu, Xiaofeng Niu
    Abstract:

    Cavidine, a major alkaloid compound isolated from Corydalis impatiens, has various pharmacological effects but its effect on Gastric Ulcer has not been previously explored. The current study aimed to investigate the possible anti-Ulcerogenic potential of cavidine in the model of Ethanol-Induced Gastric Ulcer. Mice received cavidine (1, 5 or 10mg/kg, ig), cimetidine (CMD, 100mg/kg, ig) or vehicle at 12h and 1h before absolute ethanol administration (0.5mL/100g), and animals were euthanized 3h after ethanol ingestion. Gross and histological Gastric lesions, biochemical, immunological and Western blot parameters were taken into consideration. The results showed that ethanol administration produced apparent mucosal injuries with morphological and histological damage, whereas cavidine pre-treatment reduced the Gastric injuries. Cavidine pre-treatment also ameliorated the contents of malonaldehyde (MDA) and myeloperoxidase (MPO) activity, and increased the mucosa levels of glutathione (GSH), superoxide dismutase (SOD) and prostaglandin E2 (PGE2), relative to the model group. Also cavidine was able to decrease the levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), inhibit the up-regulation of cyclo-oxygenase-2 (COX-2) expression and activation of Nuclear factor-kappa B (NF-κB) pathway. Taken together, these results indicated that cavidine exerts a gastroprotective effect against Gastric Ulceration, and the underlying mechanism might be associated with the stimulation of PGE2, reduction of oxidative stress, suppression of NF-κB expression and subsequent reduced COX-2 and pro-inflammatory cytokines.

  • Protective effect of δ-amyrone against Ethanol-Induced Gastric Ulcer in mice
    Immunobiology, 2014
    Co-Authors: Huan Yao, Xiaofeng Niu, Yu Wang, Hailin Zhang
    Abstract:

    The purpose of this study is to examine the protective effect of δ-amyrone on Ethanol-Induced Gastric Ulcer in mice. The mice intraGastric administration 75% (0.5 mL/100g) ethanol was pretreated with δ-amyrone (4 and 8 mg/kg) and cimetidine (100 mg/kg) or vehicles in different experimental groups for a continuous three-day, and animals were euthanized 3h after ethanol ingestion. The Gastric lesions were significantly attenuated by δ-amyrone (4 and 8 mg/kg) as compared to the Ulcer control group. Pre-treatment with δ-amyrone prevented the myeloperoxidase (MPO) activity, production of nitric oxide (NO) in serum, expression of inducible nitric oxide synthase (iNOS) and nuclear factor kappa B (NF-κB) p65 protein expression. Analysis of cytokines in Gastric tissue and serum of Ethanol-Induced mice showed the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were decreased by δ-amyrone in response to NF-κB p65. These results suggested that δ-amyrone exerts its protective effect on experimental Gastric Ulcer by inhibiting NF-κB signaling pathways, which subsequently reduces overproduction of the inducible enzymes iNOS and suppresses the release of the inflammatory factors TNF-α, IL-6 and NO. Thus, δ-amyrone shows promise as a therapeutic agent in experimental Gastric Ulcer.

  • Protective effect of chelerythrine against Ethanol-Induced Gastric Ulcer in mice.
    Chemico-biological interactions, 2013
    Co-Authors: Ding-jun Hao, Huimin Huang, Ting Fan, Huan Yao, Xiaofeng Niu
    Abstract:

    The quaternary benzo[c]phenanthridine alkaloid, chelerythrine (CHE), is of great practical and research interest because of its pronounced, widespread physiological effects, primarily antimicrobial and anti-inflammatory, arising from its ability to interact with proteins and DNA. Although CHE was originally shown to possess anti-inflammatory properties, its effects on acute Gastric Ulcer have not been previously explored. The aim of the present study is to evaluate the protective effect of CHE on ethanol induced Gastric Ulcer in mice. Administration of CHE at doses of 1, 5 and 10mg/kg bodyweight prior to ethanol ingestion dose-dependently inhibited Gastric Ulcer. The Gastric mucosal lesion was assessed by Ulcer area, Gastric juice acidity, myeloperoxidase (MPO) activities, macroscopic and histopathological examinations. CHE significantly reduced the Gastric Ulcer index, myeloperoxidase activities, macroscopic and histological score in a dose-dependent manner. In addition, CHE also significantly inhibited nitric oxide (NO) concentration, pro-inflammatory interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) level in serum and Gastric mucosal in the mice exposed to ethanol induced Ulceration in a dose-dependent manner. In addition, immunohistochemical analysis revealed that CHE markedly attenuated the overexpression of nuclear factor-κB in Gastric mucosa of mice. It was concluded that CHE represents a potential therapeutic option to reduce the risk of Gastric Ulceration. In addition, acute toxicity study revealed no abnormal sign to the mice treated with CHE (15mg/kg). These findings suggest that the gastroprotective activity of CHE might contribute in adjusting the inflammatory cytokine by regulating the NF-κB signalling pathway.

  • Protective effect of tetrahydrocoptisine against Ethanol-Induced Gastric Ulcer in mice.
    Toxicology and applied pharmacology, 2013
    Co-Authors: Huimin Huang, Xiaofeng Niu, Ting Fan
    Abstract:

    Excessive alcohol consumption can lead to Gastric Ulcer and the present work was aimed to examine the protective effect of tetrahydrocoptisine (THC) in the model of Ethanol-Induced Gastric Ulcer in mice. Fasted mice treated with ethanol 75% (0.5ml/100g) were pre-treated with THC (10 or 20mg/kg, ip), cimetidine (100mg/kg, ip) or saline in different experimental sets for a period of 3days, and animals were euthanized 4h after ethanol ingestion. Gross and microscopic lesions, immunological and biochemical parameters were taken into consideration. The results showed that ethanol induced Gastric damage, improving nitric oxide (NO) level, increased pro-inflammatory cytokine (TNF-α and IL-6) levels and myeloperoxidase (MPO) activity, as well as the expression of nuclear factor-κB (NF-κB) in the ethanol group. Pretreatment of THC at doses of 10 and 20mg/kg bodyweight significantly attenuated the Gastric lesions as compared to the ethanol group. These results suggest that the gastroprotective activity of THC is attributed to reducing NO production and adjusting the pro-inflammatory cytokine, inhibited neutrophil accumulation and NF-κB expression.

Snehasikta Swarnakar - One of the best experts on this subject based on the ideXlab platform.

  • Protective Role of Black Tea Flavonoids Against Ethanol-Induced Gastropathy via Matrix Metalloproteinase Pathway
    Indian Journal of Clinical Biochemistry, 2019
    Co-Authors: Susri Raychaudhuri, Satyabrata Ghosh, Anirban Roy, Snehasikta Swarnakar
    Abstract:

    Tea polyphenols are known to prevent various ailments like cancer, atherosclerosis, hypertension and diabetes. Our study aimed at to decipher the gastroprotective effect of aqueous black tea extract (BTE) against Ethanol-Induced Gastric damage and the role of BTE in modulating MMP-9 activity and expression, both in vivo and in vitro. The protective role of BTE was assessed in Sprague–Dawley rats after inducing damage with 70% ethanol. Human Gastric adenocarcinoma cells (AGS) were treated with ethanol in ex vivo experiment. MMP-9 activity and expression were investigated through gelatin zymography and western blotting. Reactive oxygen species (ROS) generation was also studied by fluorescence spectroscopy and confocal microscopy, with or without treatment of BTE both in vivo and in vitro experiments. In addition, the effect of citric acid treated BTE (cBTE), which mimics lemon tea, was examined on Ethanol-Induced gastropathy. BTE exhibited antiUlcer activity through reduction of glutathione depletion, lipid peroxidation, protein oxidation, ROS production and inflammatory cell infiltration in rat Gastric tissues. In addition, BTE significantly inhibited synthesis and secretion of proMMP-9 both in vivo and in vitro. The mitochondrial enzymes succinate dehydrogenase and NADH oxidase in rat Gastric tissues were downregulated by BTE while protecting Gastric Ulcer. Citric acid addition to BTE was observer to enrich the lead compound, catechin. Interestingly, cBTE showed higher anti-Ulcer activity than the untreated one. BTE shows protective role against Ethanol-Induced Gastric Ulcer in rats through scavenging ROS and downregulating proMMP-9 activity. While cBTE shows better protection due to enrichment of catechin and removal of tannins in tea extract leading to enhanced inhibitory role on proMMP-9 activity and ROS production. Graphical Abstract

  • The use of nano-quercetin to arrest mitochondrial damage and MMP-9 upregulation during prevention of Gastric inflammation induced by ethanol in rat.
    Biomaterials, 2012
    Co-Authors: Somsuta Chakraborty, Sami Stalin, Nirmalendu Das, Somsubhra Thakur Choudhury, Swarupa Ghosh, Snehasikta Swarnakar
    Abstract:

    Gastric Ulcer is a multifaceted process that involves reactive oxygen species (ROS) generation, extracellular matrix degradation and mitochondrial damage. Mitochondria play a crucial role for homeostasis of ROS and cell survival. In our study, we investigated the efficacy and mechanism of polymeric nanocapsuled quercetin (NQC) over the free quercetin (QC) molecule in prevention of Ethanol-Induced Gastric Ulcer in rat. NQC possessed significantly higher efficacy (~20 fold) than free QC while preventing Gastric Ulcers. Our data show that prior administration of NQC and/or QC significantly blocked synthesis and secretion of matrix metalloproteinase (MMP)-9 as well as infiltration of inflammatory cells and oxidative damage in rat Gastric tissues. As compared to free QC, NQC protected much better the mitochondrial integrity and size along with mitochondrial functions by controlling succinate dehydrogenase and NADH oxidase in rat Gastric tissues. In addition, both free QC and NQC down regulated PARP-1 as well as apoptosis during protection against Ethanol-Induced Gastric Ulcer. Herein, the effect of NQC was greater than QC on expression of enzymes like cyclooxygenase and nitric oxidase synthase (NOS)-2. We conclude that NQC with greater bioavailability offers significantly higher potency in downregulating MMP-9 and NOS-2 as well as oxidative stress in blocking Ethanol-Induced Gastric Ulcer.

  • Matrix metalloproteinase‐9 activity and expression is reduced by melatonin during prevention of ethanol‐induced Gastric Ulcer in mice
    Journal of pineal research, 2007
    Co-Authors: Snehasikta Swarnakar, Amartya Mishra, Krishnendu Ganguly, Anamika Vivek Sharma
    Abstract:

    Matrix metalloproteinases (MMPs) play an important role in degradation of Gastric extracellular matrix proteins. However, no reports are available on the relationship between the activity of MMPs and Gastric Ulceration induced by alcohol. Our objective was to investigate the effect of melatonin (N-acetyl-5-methoxytryptamine) on the regulation of MMP-9 and MMP-2 activities during prevention of Ethanol-Induced Gastric Ulcer. Biochemical and zymographic methods were used to analyze MMP-9 and -2 activities in Gastric tissues of Balb/c mice following induction of Gastric Ulcer by ethanol. Our studies reveal that melatonin arrested cell injury, protein carbonyl formation, and lipid peroxidation in mice during gastroprotection. Melatonin dose-dependently reduced proMMP-9 activity that was induced ( approximately 25-fold) during Ethanol-Induced Gastric Ulceration. Severity of Gastric Ulcers were correlated proportionately with increased dose of ethanol and elevated activity of proMMP-9 and -2. The reduced activities of MMP-9 and -2 were associated with reduced expression of TNF-alpha and increased expression of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2). We conclude that melatonin's ability to prevent Ethanol-Induced Gastric Ulceration in mice is related to a reduction in proMMP-9 activity and expression.

Abdel-razik H. Farrag - One of the best experts on this subject based on the ideXlab platform.

  • Gastroprotective effects of ursolic acid isolated from Ochrosia elliptica on Ethanol-Induced Gastric Ulcer in rats
    Medicinal Chemistry Research, 2020
    Co-Authors: Abdelsamed I Elshamy, Abdel-razik H. Farrag, Safaa H. Mohamed, Tarik A. Mohamed, Medhat M. Menshawy, Asmaa W. Zaglool, Thomas Efferth, Mohamed-elamir F. Hegazy
    Abstract:

    The prevention of Gastric Ulcer pathogenesis or its recurrence is currently one of the main goals of clinical and experimental studies. This study was conducted to investigate the gastroprotective effects of ursolic acid (UA) isolated from the dichloromethane/methanol extract of shoots of Ochrosia elliptica Labill. and investigated for its activity against an Ethanol-Induced Gastric Ulcer model in rats. Fifty-six female albino rats were divided into seven groups (eight rats per group): The first group served as control; group 2: absolute ethanol group (5 ml/kg. b.w.); group 3: ranitidine pretreated group (50 mg/kg, i.p.); group 4: UA (100 mg/kg, b.w.); and groups from 5 to 7 were pretreated with UA (25, 50, and 100 mg/kg, b.w.). Then, all animals were sacrificed, and the stomachs were excised for examination. Gastric mucosal injuries were assessed by gross examination, histopathology, immunohistochemistry of caspase-3, and biochemical parameters, including tissue malondialdehyde (MDA) and total antioxidant capacity (TAC) levels. The ethanol group showed severe mucosal injury compared with UA-treated animals, which grossly showed significant reductions of Ulcer areas. Histopathologically, they showed marked reductions of mucosal necrosis, edema, and leukocyte infiltrations. Significant increased TAC levels were associated with reduced MDA levels UA-treated rats. In addition, the caspase-3 activity was downregulated. These findings indicated gastroprotective effects of UA at the administered doses comparable with that observed with the control drug ranitidine through suppression of mucosal oxidative stress and antiapoptotic activities. Molecular docking studies showed that UA binds to H^+/K^+ ATPase more than omeprazole and ranitidine −9.23 vs. −7.09 and 6.29 kcal/mol). In addition, the pKi value of UA was also lower than omeprazole and ranitidine (169.6 nM vs. 6.3 and 26.7 µM). The molecular docking findings suggested the protection role of UA against Ethanol-Induced Gastric Ulcer.

  • chemical constituents and anti Ulcerogenic potential of the scales of cynara scolymus artichoke heads
    Journal of the Science of Food and Agriculture, 2013
    Co-Authors: Mahmoud I Nassar, Tahia K Mohamed, Sayed A Eltoumy, Azza Abdel M Lateef, Abdelsamed I Elshamy, Abdel-razik H. Farrag
    Abstract:

    BACKGROUND Cynara scolymus L. (Asteraseae) (artichoke) is commonly eaten as a vegetable; its leaves are frequently used in folk medicine in the treatment of hepatitis, hyperlipidaemia, obesity and dyspeptic disorders. The purpose of this study is to determine the chemical composition of the volatile oil and alcoholic extract of artichoke head scales. In addition, the role of the methanol extract as an anti-Ulcer agent against Ethanol-Induced Gastric Ulcer in rats was evaluated. RESULTS Six flavonoids and one phenolic acid were obtained from the methanol extract. Also, 37 compounds were identified in the volatile oil, the majority including mono- and sesquiterpenes. The artichoke extracts (200 and 400 mg kg−1) significantly (P < 0.05) reduced the Ulcer index (55.33% and 72.14% inhibition). Histopathological examination of rat stomachs demonstrated that artichoke induced an increase in Gastric mucus production, and a reduction of the depth and severity of mucosal lesions. Artichoke dose-dependently reduced the elevated ethanol Gastric malonylaldehyde, and reduced glutathione levels and catalase activity. These results suggest that the head scales of artichoke possess potential anti-Ulcer activity. CONCLUSIONS The present paper describes the identification of volatile oil for the first time along with the isolation and identification of the constituents of the methanol extract. Moreover, the high anti-Ulcerogenic potential of scales of C. scolymus heads was established here for the first time. © 2013 Society of Chemical Industry

  • Chemical constituents and anti‐Ulcerogenic potential of the scales of Cynara scolymus (artichoke) heads
    Journal of the Science of Food and Agriculture, 2013
    Co-Authors: Mahmoud I Nassar, Tahia K Mohamed, Azza Abdel M Lateef, Sayed A. El-toumy, Abdelsamed I Elshamy, Abdel-razik H. Farrag
    Abstract:

    BACKGROUND Cynara scolymus L. (Asteraseae) (artichoke) is commonly eaten as a vegetable; its leaves are frequently used in folk medicine in the treatment of hepatitis, hyperlipidaemia, obesity and dyspeptic disorders. The purpose of this study is to determine the chemical composition of the volatile oil and alcoholic extract of artichoke head scales. In addition, the role of the methanol extract as an anti-Ulcer agent against Ethanol-Induced Gastric Ulcer in rats was evaluated. RESULTS Six flavonoids and one phenolic acid were obtained from the methanol extract. Also, 37 compounds were identified in the volatile oil, the majority including mono- and sesquiterpenes. The artichoke extracts (200 and 400 mg kg−1) significantly (P < 0.05) reduced the Ulcer index (55.33% and 72.14% inhibition). Histopathological examination of rat stomachs demonstrated that artichoke induced an increase in Gastric mucus production, and a reduction of the depth and severity of mucosal lesions. Artichoke dose-dependently reduced the elevated ethanol Gastric malonylaldehyde, and reduced glutathione levels and catalase activity. These results suggest that the head scales of artichoke possess potential anti-Ulcer activity. CONCLUSIONS The present paper describes the identification of volatile oil for the first time along with the isolation and identification of the constituents of the methanol extract. Moreover, the high anti-Ulcerogenic potential of scales of C. scolymus heads was established here for the first time. © 2013 Society of Chemical Industry