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Carlos Roberto Padovani - One of the best experts on this subject based on the ideXlab platform.
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Intradermo-reação cervical para diagnóstico de infecções por Eurytrema sp em bovinos
Brazilian Journal of Veterinary Research and Animal Science, 1996Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Maria Terezinha Serrão Peraçoli, Carlos Roberto PadovaniAbstract:Cervical, intradermal reactions (IDR) were performed in 101, male and female, adult cattle slaughtered in the Vicosa abattoir Each animal received simultaneously and randomly 0,1ml of salina and salina extract of Eurytrema sp antigen in the following protein concentrations: 25(jg/ml, 50|jg/ml, 100pg/ml, 250Mg/ml, 500pg/ml and 1 mg/ml. Immediately after the injections, as well as 30 minutes later, the millimetered wheals were measured by means of a millimetered ruler in order to get the mean diameter in both times (D, initial mean diameter; Di, final mean diameter). Then, Di - D, was calculated, and in the next day each pancreas was macroscopically examined in order to investigate the parasitic condition. The following conclusions were reached: 1) all IDR showing wheal increase of 2.5mm might be considered positive, whereas the other ones (with Di - D, < 2.5mm) might be regarded as negative; 2) Eurytrema sp antigen, at the moment of its use, ought to have a concentration of 250|jg/ml of protein/ml.
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Alterações pancreáticas em bovinos naturalmente infectados por Eurytrema sp e sua associação com a carga parasitária e o número de ovos por grama de fezes (OPG)
Brazilian Journal of Veterinary Research and Animal Science, 1994Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Carlos Roberto Padovani, Maria Cecília Rui LuvizottoAbstract:Foram examinados, ao microscopio optico, fragmentos de 41 pâncreas de bovinos naturalmente infectados por Eurytrema sp e as lesoes encontradas foram classificadas em quatro graus, de acordo com sua extensao desde o epitelio dos ductos. Em seguida, tomando-se os valores das cargas parasitarias (CP) e o numero de ovos por grama de fezes (OPG) dos animais, em cada grau de lesao estabelecido, comprovou-se que os valores do OPG e, principalmente, da CP podem ser utilizados para caracterizar, de maneira satisfatoria, a extensao das lesoes pancreaticas.
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Distribution of the number of Eurytrema sp. eggs per gram of feces in naturally infected cattle.
Veterinary parasitology, 1993Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Carlos Roberto PadovaniAbstract:Eurytrema sp. egg counts (epg) in the feces of naturally infected cattle were performed and the technique employed showed 94.2% probability of detecting positive cases of the infection with a single examination independently of the host parasite burden. It was also demonstrated that the epg of Eurytrema sp. follows a negative binomial distribution model and is characterized by its small magnitude.
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Intensidade de parasitismo por Eurytrema sp em bovinos da mesorregião do Sudoeste Paulista
Brazilian Journal of Veterinary Research and Animal Science, 1992Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Carlos Roberto PadovaniAbstract:By macroscopic examination, 485 pancreas of cattle from Southwestern localities of Sao Paulo State were checked in order to count the Eurytrema sp specimens. It was concluded that the average parasite load is low in the region under study and the occurence ranged between 7. 2% and 9.8%.
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Estimativa da carga parasitária (Eurytrema sp) de um bovino em função do número de ovos por grama de fezes (OPG)
Brazilian Journal of Veterinary Research and Animal Science, 1992Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Carlos Roberto PadovaniAbstract:Foram utilizados os dados de 39 bovinos oriundos de cidades da mesorregiao do Sudoeste Paulista referentes a carga parasitaria (CP) e correspondente numero de ovos de Eurytrema sp por grama de fezes (OPG) para se pesquisar, por meio de estudos de regressao, um modelo matematico capaz de estimar a primeira a partir da segunda variavel. Nao obstante o fato da CP e OPG relacionarem-se gracilmente (R 2 = 38,2%), verificou-se que o objetivo referido pode ser alcancado empregando-se a seguinte relacao matematica: CP = 63,1050 (OPG + 1) 0,5616 . Nestas condicoes, pode-se prever que os casos falsos negativos (OPG=O) devem albergar, nos pâncreas, uma media de 63,1 parasitos e os casos positivos (OPG ≥ 1) media igual ou superior a 93,2 parasitos.
Pacífico Antônio Diniz Belém - One of the best experts on this subject based on the ideXlab platform.
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Atividades hidrolíticas de amostras de conteúdo do intestino delgado de bovinos adultos, infectados ou não por Eurytrema sp, determinadas por difusão radial enzimática
Ciencia Rural, 2000Co-Authors: Pacífico Antônio Diniz Belém, Carlos Batista De Assis, Marcelo Lopes De SantanaAbstract:Hidrolitic activities of small intestine content as determined by radial enzyme diffusion in agar gel containing calcium caseinate, corn oil, starch or skimmed milk were measured in 38 mature cattle from slaughterhouse. Each one were identified as normal or infected and then classified into two different grades according to pancreatic ducts lesions and parasite burden. The substrates calcium caseinate and corn oil did not shown cleared zones in several radial enzyme diffusion tests or it was difficult to get a correct measure of the diameters. Such problems, however, were not observed with starch and skimmed milk. In these cases, hidrolitic activities were similar in both control and Eurytrema sp infected groups, despite of the pancreatic duct lesions and parasite load (P>0.05). So, it was concluded that the radial enzyme diffusion method couldnt shown any exocrine pancreatic disfunction in Eurytrema sp infected cattle.
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Intradermo-reação cervical para diagnóstico de infecções por Eurytrema sp em bovinos
Brazilian Journal of Veterinary Research and Animal Science, 1996Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Maria Terezinha Serrão Peraçoli, Carlos Roberto PadovaniAbstract:Cervical, intradermal reactions (IDR) were performed in 101, male and female, adult cattle slaughtered in the Vicosa abattoir Each animal received simultaneously and randomly 0,1ml of salina and salina extract of Eurytrema sp antigen in the following protein concentrations: 25(jg/ml, 50|jg/ml, 100pg/ml, 250Mg/ml, 500pg/ml and 1 mg/ml. Immediately after the injections, as well as 30 minutes later, the millimetered wheals were measured by means of a millimetered ruler in order to get the mean diameter in both times (D, initial mean diameter; Di, final mean diameter). Then, Di - D, was calculated, and in the next day each pancreas was macroscopically examined in order to investigate the parasitic condition. The following conclusions were reached: 1) all IDR showing wheal increase of 2.5mm might be considered positive, whereas the other ones (with Di - D, < 2.5mm) might be regarded as negative; 2) Eurytrema sp antigen, at the moment of its use, ought to have a concentration of 250|jg/ml of protein/ml.
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Alterações pancreáticas em bovinos naturalmente infectados por Eurytrema sp e sua associação com a carga parasitária e o número de ovos por grama de fezes (OPG)
Brazilian Journal of Veterinary Research and Animal Science, 1994Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Carlos Roberto Padovani, Maria Cecília Rui LuvizottoAbstract:Foram examinados, ao microscopio optico, fragmentos de 41 pâncreas de bovinos naturalmente infectados por Eurytrema sp e as lesoes encontradas foram classificadas em quatro graus, de acordo com sua extensao desde o epitelio dos ductos. Em seguida, tomando-se os valores das cargas parasitarias (CP) e o numero de ovos por grama de fezes (OPG) dos animais, em cada grau de lesao estabelecido, comprovou-se que os valores do OPG e, principalmente, da CP podem ser utilizados para caracterizar, de maneira satisfatoria, a extensao das lesoes pancreaticas.
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Curso natural da eliminação de ovos de Eurytrema sp LOSS, 1907 nas fezes de bezerros
Brazilian Journal of Veterinary Research and Animal Science, 1994Co-Authors: Jackson Victor De Araújo, Pacífico Antônio Diniz BelémAbstract:Treze bezerros 3/4 holandes x 1/4 zebu, nascidos em diferentes estacoes do ano de 1989 e criados intensivamente, foram transferidos para pastagens de capim gordura (Melinis minutiflora ) entre 7 meses e 1 ano de idade, no final do periodo chuvoso (marco de 1990), a fim de ser acompanhado o curso natural da eliminacao de ovos de Eurytrema sp por grama de fezes (OPG). De 15 em 15 dias, foram feitas as colheitas de fezes para obter as contagens de OPG durante 18 meses. Os primeiros animais que apresentaram exames positivos (a partir de agosto de 1990) foram os que tiveram as maiores contagens de OPG durante o periodo experimental. Os demais apresentaram os primeiros exames positivos em diferentes ocasioes e as contagens de OPG destes foram baixas e oscilantes. Tres animais apresentaram exames negativos. Provavelmente, a maioria dos bovinos criados em regime de pastejo desta regiao deverao apresentar infeccao parasitaria por Eurytrema sp em alguma fase de suas vidas.
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Distribution of the number of Eurytrema sp. eggs per gram of feces in naturally infected cattle.
Veterinary parasitology, 1993Co-Authors: Pacífico Antônio Diniz Belém, Mauro Rodrigues De Oliveira, Carlos Roberto PadovaniAbstract:Eurytrema sp. egg counts (epg) in the feces of naturally infected cattle were performed and the technique employed showed 94.2% probability of detecting positive cases of the infection with a single examination independently of the host parasite burden. It was also demonstrated that the epg of Eurytrema sp. follows a negative binomial distribution model and is characterized by its small magnitude.
Claiton I. Schwertz - One of the best experts on this subject based on the ideXlab platform.
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Comments on Eurytrematosis in Brazil and the possibility of human infection.
World journal of experimental medicine, 2017Co-Authors: Claiton I. Schwertz, Luan Cleber Henker, Ricardo E. MendesAbstract:The manuscript "Eurytrematosis: An emerging and neglected disease in South Brazil" discusses some aspects of Eurytrema sp. fluke as an animal pathogen and based in some aspects of the parasitism in cattle and the life cycle of Eurytrema sp. Authors suggest the possibility of human infection, once there is no research on this subject in Brazil. In human cases reported, the mechanism of infection was not disclosed, so it keeps the discussion opened. Although we focused on animal Eurytrematosis, we speculated the possibility of human infection by Eurytrema sp. in Brazil, but after all, the only way to determine it, would be a study searching for people infected through coprological or serological tests.
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Cattle naturally infected by Eurytrema coelomaticum: Relation between adenosine deaminase activity and zinc levels
Research in veterinary science, 2016Co-Authors: Hyolanda M. Grosskopf, Claiton I. Schwertz, Neuber J. Lucca, Mateus Eloir Gabriel, N B Bottari, G Machado, Maria Rosa Chitolina Schetinger, Ester S. Da Silva, Mariana S. Alves, V M MorschAbstract:Abstract The enzyme adenosine deaminase (ADA) is critical for modulating the immune system, and in the presence of zinc, its activity is catalyzed. The aim of this study was to evaluate the ADA activity in pancreas of cattle naturally infected by Eurytrema coelomaticum in relation to the results of zinc levels, pathological findings and parasite load. For this study 51 slaughtered cattle were used. The animals were divided into two groups: Group A consisting of animals naturally infected by E. coelomaticum (n = 33) and Group B of uninfected animals (n = 18). Blood and pancreas were collected of each animal for analysis of zinc and ADA, respectively. Infected cattle showed a reduction on seric levels of zinc, and decreased ADA activity in the pancreas (P > 0.05). A positive correlation between zinc levels and ADA activity was observed. Thus, high parasite load and severity of histopathologic lesions affect the ADA activity in pancreas, as well as the zinc levels in serum of infected animals (negative correlation between these variables). Therefore, we can conclude that cattle infected by E. coelomaticum have low ADA activity in pancreas, which can be directly related to zinc reduction, responsible for ADA activation and catalyzes.
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NTPDase and 5′-nucleotidase as inflammatory markers in cattle naturally infected by Eurytrema coelomaticum
Comparative immunology microbiology and infectious diseases, 2016Co-Authors: Juscivete F. Fávero, Claiton I. Schwertz, Mateus Eloir Gabriel, G Machado, Ester S. Da Silva, Pedro H. Doleski, Daniela B.r. Leal, Alessandra G. Manzoni, Fernanda A. Stedille, Ricardo ChristAbstract:The aim of this study was to evaluate seric NTPDase and 5′nucleotidase activities of cattle naturally infected by Eurytrema coelomanticum, as well as to correlate them to histopathological lesions in the pancreas and the degree of parasitism. Blood samples and pancreas of 51 bovines were collected on a slaughterhouse in Southern Brazil: 33 from cattle naturally infected by E. coelomanticum (the Group A), and 18 from uninfected animals (the Group B). Infected animals showed an average of 532 parasites per pancreas. In the pancreatic histology, ducts displayed hyperplasia, stenosis, proliferation of fibrous tissue, and interstitial inflammatory infiltration of lymphocytes. The serum from infected animals showed an increase in NTPDase activity when ATP was used as substrate (P < 0.001). For the ADP substrate, there was no difference between groups regarding NTPDase activity (P = 0.37), as well as 5′-nucleotidase activity (P = 0.27). Correlating NTPDase activity (ATP substrate) with the degree of histopathological lesions (rho = 0.66, P < 0.001) and the parasitic load on the pancreas (rho = 0.65, P < 0.001), a positive correlation was observed. Similar results were found between the degree of histopathological lesions and NTPDase activity (ADP substrate; rho = 0.29, P = 0.03), and 5′nucleotidase activity (rho = 0.35, P = 0.01). Based on the results of NTPDase and 5′nucleotidase enzymes in cattle naturally infected by E. coleomanticum, it is possible to suggest that these enzymes are involved in the modulation of inflammation, and they can act as markers of inflammatory response.
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Oxidative stress associated with pathological changes in the pancreas of cattle naturally infected by Eurytrema coelomaticum.
Veterinary parasitology, 2016Co-Authors: Claiton I. Schwertz, Luan Cleber Henker, Mateus Eloir Gabriel, N B Bottari, N Dos S Guarda, R N Moresco, G Machado, V M Morsch, Guilherme Do Carmo, Maria Rosa Chitolina SchetingerAbstract:Although Eurytrema coelomaticum is considered a parasite with low pathogenicity, it may be associated with mortality and loss of productive performance in animals due to chronic pancreatitis. The aim of this study was to evaluate the occurrence of oxidative stress caused by E. coelomaticum in naturally infected cattle, correlating the biochemical findings with the parasite load and histopathological changes. For this study, blood and pancreas samples from 51 cattle were collected, and levels of the thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPP) and ferric reducing ability of plasma (FRAP) were measured in the serum and pancreas, and superoxide dismutase (SOD) activity was measured in total blood. Parasite burden was determined opening the pancreatic ducts, and then fragments of pancreas were collected and fixed in 10% buffered formalin and routinely processed for histopathology. From the 51 collected pancreas, 33 (63.5%) were parasitized. The average parasite burden per pancreas was 532 (12-2,578). TBARS and FRAP showed higher levels in serum and pancreas of infected animals (p
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relationship between pathological findings and cholinesterase activity and nitric oxide levels in cattle infected naturally by Eurytrema coelomaticum
Journal of Comparative Pathology, 2016Co-Authors: Claiton I. Schwertz, Neuber J. Lucca, N B Bottari, E S Da Silva, M E Gabriel, R N Moresco, G Machado, G Do M Carmo, Dos N S Guarda, V M MorschAbstract:The aim of this study was to evaluate the role of butyrylcholinesterase (BChE) (in the serum and pancreas), acetylcholinesterase (AChE) (in the whole blood and pancreas) and nitric oxide (NO) (in the serum and pancreas) in cattle infected naturally by Eurytrema coelomaticum. Fifty-one cattle were studied, including 33 infected by E. coelomaticum and 18 uninfected animals. Significantly greater AChE activity was found in the pancreas of infected animals (P <0.01); however, these cattle had lower AChE activity in whole blood. BChE activity was greater in the sera of infected animals (P = 0.05), but was less in pancreatic samples. NO levels were significantly higher in the sera (P <0.05) and pancreas (P <0.001) of infected cattle compared with uninfected animals. A positive correlation was found between AChE activity in the pancreas and parasite load, but there was negative correlation between pancreatic BChE activity and parasitic load. Expression of AChE, BChE and NO is therefore linked to the inflammation caused by E. coelomaticum in cattle.
Ricardo E. Mendes - One of the best experts on this subject based on the ideXlab platform.
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Comments on Eurytrematosis in Brazil and the possibility of human infection.
World journal of experimental medicine, 2017Co-Authors: Claiton I. Schwertz, Luan Cleber Henker, Ricardo E. MendesAbstract:The manuscript "Eurytrematosis: An emerging and neglected disease in South Brazil" discusses some aspects of Eurytrema sp. fluke as an animal pathogen and based in some aspects of the parasitism in cattle and the life cycle of Eurytrema sp. Authors suggest the possibility of human infection, once there is no research on this subject in Brazil. In human cases reported, the mechanism of infection was not disclosed, so it keeps the discussion opened. Although we focused on animal Eurytrematosis, we speculated the possibility of human infection by Eurytrema sp. in Brazil, but after all, the only way to determine it, would be a study searching for people infected through coprological or serological tests.
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Eurytrematosis: An emerging and neglected disease in South Brazil.
World journal of experimental medicine, 2015Co-Authors: Claiton I. Schwertz, Neuber J. Lucca, Aleksandro Schafer Da Silva, Piotr Baska, Gustavo Bonetto, Mateus Eloir Gabriel, Fábio Centofanti, Ricardo E. MendesAbstract:The trematodes of the genus Eurytrema are low pathogenic pancreatic parasites, but can be related to a decrease in cattle productive performance and eventually death. Parasitized animals develop chronic interstitial pancreatitis and may show a productive performance drop and emaciation. Human infection by Eurytrema sp. has already been reported in other countries as an incidental finding during autopsy or routine tests, but the parasite has not been found in humans in Brazil. However, it is possible that a large number of people could be infected, since parasitological tests have low sensitivity and the parasite is neglected as a pathogen for humans and even animals. Attempts to control and treat Eurytrematosis have generally presented low effectiveness. With the aim to control the disease and provide more information regarding its pathogenicity, our research group is developing a number of studies about Eurytrema spp. We hope to determine the damage in productivity, as well as, establish an efficient protocol for treatment and control of Eurytrematosis based on immunoprophylaxis and antiparasitical drug therapy.
Selwyn Arlington Headley - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia , 2014Co-Authors: Gustavo Freire Figueira, Victor H.s. Oliveira, Alessandra Taroda, Amauri Alcindo Alfieri, Selwyn Arlington HeadleyAbstract:This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.
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molecular characterization of Eurytrema coelomaticum in cattle from parana brazil caracterizacao molecular de Eurytrema coelomaticum em bovinos do estado do parana brasil
2014Co-Authors: Gustavo Freire Figueira, Alessandra Taroda, Amauri Alcindo Alfieri, Victor Henrique, Silva De Oliveira, Selwyn Arlington HeadleyAbstract:4 * 1 Abstract This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.
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Epidemiology of Eurytrematosis (Eurytrema spp. Trematoda: Dicrocoeliidae) in slaughtered beef cattle from the central-west region of the State of Paraná, Brazil.
Veterinary Parasitology, 2006Co-Authors: Clóvis Antonio Bassani, Milton Hissashi Yamamura, João Paulo Elsen Saut, Luís Antônio Sangioni, Selwyn Arlington HeadleyAbstract:Bovine Eurytrematosis (BE) in Brazil is caused by Eurytrema coelomaticum; the disease results in the condemnation of the pancreas of infected animals. The objective of this study was to describe the epidemiology of Eurytrematosis in cattle submitted for slaughter during June 2003 to May 2004 at the municipal abattoir of Campo Mourao, Parana, Brazil, and compare gross observation of infection with histological findings. During the period studied, 1828 pancreases were collected randomly. The average prevalence was 47.8% (874/1828); prevalence varied from 26.9% (46/171) to 72.9% (105/144). It was discovered that the quantity of parasites within the interlobular pancreatic ducts was directly proportional to pancreatic destruction; females were more predominately infected relative to males; BE is endemic within the region of Campo Mourao; the average age of infection increases proportionally with prevalence; gross evaluation of bovine Eurytrematosis is not efficient to characterize infection by E. coelomaticum in susceptible animals.
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Simultaneous chronic seneciosis and Eurytrematosis in a cow
Semina-ciencias Agrarias, 2004Co-Authors: Selwyn Arlington Headley, João Paulo Elsen Saut, Diogini Albano Gomes, Dieggo Ricardo Meira Da Silva, Ighor Augusto De Almeida, Luiz Antonio SangioniAbstract:Chronic intoxication associated with the ingestion of Senecio brasiliensis with concomitant interstitial and periductal pancreatitis induced by Eurytrema spp are described in a cow that demonstrated severe progressive emaciation as its principal clinical manifestation. In chronic seneciosis there was severe toxic hepatitis characterized by periportal and bridging fibrosis, megalocytosis, and proliferation of epithelial bile duct cells. Infestation by Eurytrema spp produced marked periductal and interstitial fibrosis associated with parasitic forms within interlobular pancreatic ducts.
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Prevalência e distribuição geográfica de euritre-matose bovina em animais abatidos no norte do Estado do Paraná
Pesquisa Veterinária Brasileira, 2004Co-Authors: Jefferson Ruela De Azevedo, Selwyn Arlington Headley, Renate Cristiane Mannigel, Adriana Z. Agulhon, Thiago Ramalho Borba, Adriana W. Barbiéri, Daniella C. L. De Oliveira, Vanderly JaneiroAbstract:A retrospective study of cattle slaughtered in northern Parana during 2000 was performed to determine the prevalence and geographical distribution of bovine Eurytrematosis (BE), as identified by the Federal Inspection Service (SIF). The cattle was from different regions of the State of Parana; all regions had cattle parasitized by Eurytrema spp. BE was identified in 12.1% (12,534/103,411) of the total number of cattle inspected. Prevalence of animals parasitized by Eurytrema spp varied from 8.3% (Region G, Sao Joao do Caiua, 1,069/12,914) to 40.5% (Region R, Ponta Grossa, 225/555). BE was more prevalent during the month of March (1.6) and markedly reduced during May (-2). A possible seasonal predominance of BE was identified: comparatively fewer cases occurred from April to August, while there was a peak from December to March. The study indicates that bovine Eurytrematosis is hypoendemic and occurs in almost all geographical regions of the State of Parana. The prevalence within this State is variable and may be directly related to factors of the biological cycle of the trematode, particularities of each region, and environmental conditions.
