The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Gertrude Thompson - One of the best experts on this subject based on the ideXlab platform.
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genetic analysis of Feline Panleukopenia Virus full length vp2 gene in domestic cats between 2006 2008 and 2012 2014 portugal
Transboundary and Emerging Diseases, 2017Co-Authors: Carla Miranda, C R Parrish, M J Vieira, Eliane Silva, J Carvalheira, Gertrude ThompsonAbstract:Summary Feline Panleukopenia Virus (FPV) and canine parvoVirus (CPV) are two closely related Viruses, which cause acute gastroenteritis in carnivores, and cats may be infected by strains of both Viruses. The Viruses are found worldwide and may have changing host ranges and genetic variation that can be found around the world in some cases. Here, we screened a Portuguese population of cats by a conventional PCR assay for the presence of FPV/CPV Viruses in faecal samples and tissues between 2006–2008 and 2012–2014. The sequence analysis of the complete VP2 gene showed that 18 of 31 animals tested were positive for FPV DNA, and no case of CPV infection was detected. The analysis of specific DNA detected three new non-synonymous substitutions in the VP2 gene that were found in single groups and were related to Viruses reported elsewhere by phylogenetic analysis – some were related to Italian isolates, one was closely related to isolates from Vietnam and China, and two were related with older strains from the USA. The results of our study show that FPV circulated in the Portuguese cat population and as expected the found strains are slightly divergent from those reported previously.
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Genetic Analysis of Feline Panleukopenia Virus Full-length VP2 Gene in Domestic Cats Between 2006–2008 and 2012–2014, Portugal
Transboundary and emerging diseases, 2016Co-Authors: Carla Miranda, C R Parrish, M J Vieira, Eliane Silva, J Carvalheira, Gertrude ThompsonAbstract:Summary Feline Panleukopenia Virus (FPV) and canine parvoVirus (CPV) are two closely related Viruses, which cause acute gastroenteritis in carnivores, and cats may be infected by strains of both Viruses. The Viruses are found worldwide and may have changing host ranges and genetic variation that can be found around the world in some cases. Here, we screened a Portuguese population of cats by a conventional PCR assay for the presence of FPV/CPV Viruses in faecal samples and tissues between 2006–2008 and 2012–2014. The sequence analysis of the complete VP2 gene showed that 18 of 31 animals tested were positive for FPV DNA, and no case of CPV infection was detected. The analysis of specific DNA detected three new non-synonymous substitutions in the VP2 gene that were found in single groups and were related to Viruses reported elsewhere by phylogenetic analysis – some were related to Italian isolates, one was closely related to isolates from Vietnam and China, and two were related with older strains from the USA. The results of our study show that FPV circulated in the Portuguese cat population and as expected the found strains are slightly divergent from those reported previously.
C R Parrish - One of the best experts on this subject based on the ideXlab platform.
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genetic analysis of Feline Panleukopenia Virus full length vp2 gene in domestic cats between 2006 2008 and 2012 2014 portugal
Transboundary and Emerging Diseases, 2017Co-Authors: Carla Miranda, C R Parrish, M J Vieira, Eliane Silva, J Carvalheira, Gertrude ThompsonAbstract:Summary Feline Panleukopenia Virus (FPV) and canine parvoVirus (CPV) are two closely related Viruses, which cause acute gastroenteritis in carnivores, and cats may be infected by strains of both Viruses. The Viruses are found worldwide and may have changing host ranges and genetic variation that can be found around the world in some cases. Here, we screened a Portuguese population of cats by a conventional PCR assay for the presence of FPV/CPV Viruses in faecal samples and tissues between 2006–2008 and 2012–2014. The sequence analysis of the complete VP2 gene showed that 18 of 31 animals tested were positive for FPV DNA, and no case of CPV infection was detected. The analysis of specific DNA detected three new non-synonymous substitutions in the VP2 gene that were found in single groups and were related to Viruses reported elsewhere by phylogenetic analysis – some were related to Italian isolates, one was closely related to isolates from Vietnam and China, and two were related with older strains from the USA. The results of our study show that FPV circulated in the Portuguese cat population and as expected the found strains are slightly divergent from those reported previously.
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Genetic Analysis of Feline Panleukopenia Virus Full-length VP2 Gene in Domestic Cats Between 2006–2008 and 2012–2014, Portugal
Transboundary and emerging diseases, 2016Co-Authors: Carla Miranda, C R Parrish, M J Vieira, Eliane Silva, J Carvalheira, Gertrude ThompsonAbstract:Summary Feline Panleukopenia Virus (FPV) and canine parvoVirus (CPV) are two closely related Viruses, which cause acute gastroenteritis in carnivores, and cats may be infected by strains of both Viruses. The Viruses are found worldwide and may have changing host ranges and genetic variation that can be found around the world in some cases. Here, we screened a Portuguese population of cats by a conventional PCR assay for the presence of FPV/CPV Viruses in faecal samples and tissues between 2006–2008 and 2012–2014. The sequence analysis of the complete VP2 gene showed that 18 of 31 animals tested were positive for FPV DNA, and no case of CPV infection was detected. The analysis of specific DNA detected three new non-synonymous substitutions in the VP2 gene that were found in single groups and were related to Viruses reported elsewhere by phylogenetic analysis – some were related to Italian isolates, one was closely related to isolates from Vietnam and China, and two were related with older strains from the USA. The results of our study show that FPV circulated in the Portuguese cat population and as expected the found strains are slightly divergent from those reported previously.
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tropic determinant for canine parvoVirus and Feline Panleukopenia Virus functions through the capsid protein vp2
Journal of General Virology, 1997Co-Authors: Austin L Spitzer, C R Parrish, Ian H MaxwellAbstract:Canine parvoVirus (CPV) can productively infect canine and Feline cell lines whereas Feline Panleukopenia Virus (FPV) is restricted to the latter. The major determinants of tropism are two amino acids in the sequence shared by the capsid proteins, VP1 and VP2. We have shown that a rodent parvoVirus-derived transducing genome, containing the luciferase reporter, can be packaged by VP1 and VP2 from separate helper sources. Canine A72 cells and Feline CFK cells were transduced with recombinant virions generated using VP1 and VP2 combinations from CPV and FPV. Both VP1 and VP2 were necessary for production of transducing virions. Efficient transduction of A72 cells required VP2 of CPV. Therefore, the capsid determinants of tropism for CPV and FPV are in VP2, although a source of VP1 is also necessary to produce infectious particles. The results extend similar observations on the tropic determinants of different strains of minute Virus of mice.
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3 pathogenesis of Feline Panleukopenia Virus and canine parvoVirus
Baillière's clinical haematology, 1995Co-Authors: C R ParrishAbstract:Summary Feline Panleukopenia Virus (FPV) and canine parvoVirus (CPV) are autonomous parvoViruses which infect cats or dogs, respectively. Both Viruses cause an acute disease, with Virus replicating for less than seven days before being cleared by the developing immune responses. The Viruses have a broad tropism for mitotically active cells. In neonatal animals the Viruses replicate in a large number of tissues, and FPV infection of the germinal epithelium of the cerebellum leads to cerebellar hypoplasia, while CPV may infect the hearts of neonatal pups, causing myocarditis. In older animals the Virus replicates systemically, primarily in the primary and secondary lymphoid tissues, and also in the rapidly replicating cells of the small intestinal epithelial crypts. A transient Panleukopenia or relative lymphopenia is often observed after FPV or CPV infection, respectively. Whether the reduction in cell numbers in vivo is due to Virus replicating in and killing cells, or due to other indirect effects, is not known. However, FPV kills both erythroid and myeloid colony progenitors in in vitro bone marow cultures, and it has been suggested that Virus replication in the myeloid cells in vivo could lead to the reduced neutrophil levels seen after FPV infection of cats.
Fred W Scott - One of the best experts on this subject based on the ideXlab platform.
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raccoon poxVirus live recombinant Feline Panleukopenia Virus vp2 and rabies Virus glycoprotein bivalent vaccine
Vaccine, 1997Co-Authors: Liangbiao Hu, Christopher K Ngichabe, Charles V Trimarchi, Joseph J Esposito, Fred W ScottAbstract:Abstract A raccoon poxVirus (RCNV) recombinant for immunizing against Feline Panleukopenia and rabies was developed by homologous recombination with a chimeric plasmid for insertional inactivation of the RCNV thymidine kinase gene. The recombinant, RCN-FPV/VP2-rabG, coexpressed the Feline Panleukopenia Virus (FPV) VP2 protein and the rabies Virus spike glycoprotein (rabG) under oppositely oriented vaccinia Virus P11 promoters. Cats vaccinated subcutaneously with the recombinant showed relatively high neutralizing antibody responses against rabies Virus and FPV, and protection against an otherwise virulent FPV challenge with no drop in white blood cell count. Because of containment constraints, no rabies Virus challenges were done, but the high concentrations (> 8 IU) of rabies neutralizing antibodies were consistent with levels that usually indicate an ability to counter the infection.
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raccoon poxVirus Feline Panleukopenia Virus vp2 recombinant protects cats against fpv challenge
Virology, 1996Co-Authors: Liangbiao Hu, Joseph J Esposito, Fred W ScottAbstract:Abstract An infectious raccoon poxVirus (RCNV) was used to express the Feline Panleukopenia Virus (FPV) open reading frame VP2. The recombinant, RCNV/FPV, was constructed by homologous recombination with a chimeric plasmid for inserting the expression cassette into the thymidine kinase (TK) locus of RCNV. Expression of the VP2 DNA was regulated by the vaccinia Virus late promoter P 11 . Southern blot and polymerase chain reaction (PCR) analyses confirmed the cassette was in the TK gene of the RCNV genome. An immunofluorescent antibody assay using Feline anti-FPV polyclonal serum showed the expressed viral antigen in the cytoplasm of infected cells. Radioimmunoprecipitation with the same antiserum detected a 67-kDa VP2 protein which exactly matched the migration of the authentic FPV VP2 protein by SDS–polyacrylamide gel electrophoresis. Nine five-month-old cats were vaccinated and 21 days later were boosted with the recombinant Virus. Peroral FPV challenge 2 weeks after the booster showed that the cats were fully protected as measured by examining clinical signs and total white blood cell counts in peripheral blood. Cats not immunized developed low to very low leukocyte counts following peroral FPV challenge. The nine vaccinated cats showed high FPV neutralization antibody prior to challenge, whereas nonvaccinated cats formed anti-FPV antibodies only after challenge.
Uwe Truyen - One of the best experts on this subject based on the ideXlab platform.
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antibody response to Feline Panleukopenia Virus vaccination in healthy adult cats
Journal of Feline Medicine and Surgery, 2017Co-Authors: Michèle Bergmann, Stephanie Schwertler, Uwe Truyen, Stephanie Speck, Sven Reese, Katrin HartmannAbstract:ObjectivesAccording to prior studies, between 25.0% and 92.8% of adult cats have antibodies against Feline Panleukopenia Virus (FPV) and thus are likely protected against FPV infection. It is, however, unknown how healthy adult cats with different antibody titres react to FPV vaccination in the field. Therefore, the aim of the study was to measure antibody titres in healthy adult cats within a period of 28 days after vaccination against FPV and to evaluate factors that are associated with a lack of adequate response to vaccination.MethodsOne hundred and twelve healthy adult cats were vaccinated with a vaccine against FPV, Feline herpesVirus and Feline caliciVirus. Antibodies against FPV were determined before vaccination (day 0), on day 7 and day 28 after vaccination by haemagglutination inhibition (HI). A HI titre ⩾1:40 was defined as protective. An adequate response to vaccination was defined as a four-fold titre increase. Uni- and multivariate statistical analysis was used to determine factors associat...
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prevalence of antibodies against Feline Panleukopenia Virus in client owned cats in southern germany
Veterinary Journal, 2014Co-Authors: Katherina Mende, Bianca Stuetzer, Timo Homeier, Carola Sauterlouis, Uwe Truyen, Katrin HartmannAbstract:Abstract Feline Panleukopenia is a frequent and commonly fatal disease of cats. Recent published studies have raised suspicions that some cats fail to develop antibodies after vaccination. The purpose of this study was to assess the prevalence of antibodies against Feline Panleukopenia Virus (FPV) in cats in Southern Germany, and to identify factors that are associated with a lack of antibodies. In total, 350 cats presented to the Clinic of Small Animal Medicine, Ludwig-Maximilians-Universitaet were randomly included in the study. Information regarding signalment, origin, environment, lifestyle, housing conditions, health status, chronic diseases, glucocorticoid therapy, and vaccination status were collected. Antibodies were detected by haemagglutination inhibition test. Asymptomatic chi-squared tests and univariable logistic regression were used to investigate associations between a lack of antibodies and the different variables. Associations determined to be statistically significant at P Of the 350 cats, 103 (29.4%) had no antibodies against FPV. Chronic kidney disease, neoplasia, glucocorticoid therapy, and vaccination status were significantly associated with a lack of antibodies. The cats with no antibodies were likely to have inadequate immunity against Panleukopenia and those with chronic diseases or receiving glucocorticoids were less likely to be protected.
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prevalence of antibodies against Feline Panleukopenia Virus in client owned cats in southern germany
Veterinary Journal, 2014Co-Authors: Katherina Mende, Bianca Stuetzer, Timo Homeier, Carola Sauterlouis, Uwe Truyen, Katrin HartmannAbstract:Feline Panleukopenia is a frequent and commonly fatal disease of cats. Recent published studies have raised suspicions that some cats fail to develop antibodies after vaccination. The purpose of this study was to assess the prevalence of antibodies against Feline Panleukopenia Virus (FPV) in cats in Southern Germany, and to identify factors that are associated with a lack of antibodies. In total, 350 cats presented to the Clinic of Small Animal Medicine, Ludwig-Maximilians-Universitaet were randomly included in the study. Information regarding signalment, origin, environment, lifestyle, housing conditions, health status, chronic diseases, glucocorticoid therapy, and vaccination status were collected. Antibodies were detected by haemagglutination inhibition test. Asymptomatic chi-squared tests and univariable logistic regression were used to investigate associations between a lack of antibodies and the different variables. Associations determined to be statistically significant at P<0.1 were verified by a multivariable logistic regression analysis. Of the 350 cats, 103 (29.4%) had no antibodies against FPV. Chronic kidney disease, neoplasia, glucocorticoid therapy, and vaccination status were significantly associated with a lack of antibodies. The cats with no antibodies were likely to have inadequate immunity against Panleukopenia and those with chronic diseases or receiving glucocorticoids were less likely to be protected.
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evaluation of an in house dot enzyme linked immunosorbent assay to detect antibodies against Feline Panleukopenia Virus
Journal of Feline Medicine and Surgery, 2014Co-Authors: Katherina Mende, Bianca Stuetzer, Uwe Truyen, Katrin HartmannAbstract:Measuring antibody titres to determine a cat’s immunity to core diseases instead of just administering annual vaccinations has not been established in Germany so far. An in-house test kit for the detection of antibodies against Feline Panleukopenia Virus (FPV), Feline herpesVirus-1 and Feline caliciVirus – the ImmunoComb Feline VacciCheck – is now available in several European countries. The aim of this study was to assess the quality of the ImmunoComb Feline VacciCheck to determine antibodies by comparing it to a gold standard. The test is aimed for use in practice to assist decision-making when performing an individual health assessment to see whether a cat is potentially unprotected against FPV and requires FPV vaccination. Sera from 347 cats were included in the study. For antibody detection, haemagglutination inhibition (HI) was performed as gold standard. Sensitivity, specificity and positive and negative predictive values of the ImmunoComb Feline VacciCheck were determined for three different HI ti...
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Evaluation of an in-house dot enzyme-linked immunosorbent assay to detect antibodies against Feline Panleukopenia Virus.
Journal of feline medicine and surgery, 2014Co-Authors: Katherina Mende, Bianca Stuetzer, Uwe Truyen, Katrin HartmannAbstract:Measuring antibody titres to determine a cat's immunity to core diseases instead of just administering annual vaccinations has not been established in Germany so far. An in-house test kit for the detection of antibodies against Feline Panleukopenia Virus (FPV), Feline herpesVirus-1 and Feline caliciVirus-- the ImmunoComb Feline VacciCheck--is now available in several European countries. The aim of this study was to assess the quality of the ImmunoComb Feline VacciCheck to determine antibodies by comparing it to a gold standard. The test is aimed for use in practice to assist decision-making when performing an individual health assessment to see whether a cat is potentially unprotected against FPV and requires FPV vaccination. Sera from 347 cats were included in the study. For antibody detection, haemagglutination inhibition (HI) was performed as gold standard. Sensitivity, specificity and positive and negative predictive values of the ImmunoComb Feline VacciCheck were determined for three different HI titre cut-off points (1:20, 1:40, 1:80). In comparison to the HI, the ImmunoComb Feline VacciCheck showed a sensitivity of 79%, 83% and 87%, and a specificity of 89%, 86% and 81%, respectively. Specificity of the ImmunoComb Feline VacciCheck, which was considered the most important parameter, was acceptable in comparison to HI. Especially when considering an antibody titre of 1:20 sufficient for protection (eg, in an adult animal), the ImmunoComb Feline VacciCheck can be recommended for use in veterinary practice.
Hua Yuping - One of the best experts on this subject based on the ideXlab platform.
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development and identification of the monoclonal antibodies against recombinant vp2 protein of Feline Panleukopenia Virus from tiger
Journal of Economic Animal, 2010Co-Authors: Tian Lihong, Hua YupingAbstract:Monoclonal antibody(McAb)3C4 against recombinant VP2 protein of Feline Panleukopenia Virus from tiger were developed by fusing myeloma cell line SP2/0 with splenocytes of BALB/c mice immunized with prokaryotic expressed VP2 protein.The positive hybridoma cells were sifted by ELISA with tiger Feline Panleukopenia Virus.The antibody titers of ascites for the hybridoma lines were 1∶12 800.The subtype of monoclonal antibodies was determined as IgG2a.Indirect immunofluorescent test indicated that the monoclonal antibody could specifically react with FPV,and it may be used as an valuable tools for diagnosis of FPV.
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development and identification of the monoclonal antibodies against recombinant vp2 protein of Feline Panleukopenia Virus from tiger
Journal of Economic Animal, 2010Co-Authors: Tian Lihong, Hua YupingAbstract:Monoclonal antibody(McAb)3C4 against recombinant VP2 protein of Feline Panleukopenia Virus from tiger were developed by fusing myeloma cell line SP2/0 with splenocytes of BALB/c mice immunized with prokaryotic expressed VP2 protein.The positive hybridoma cells were sifted by ELISA with tiger Feline Panleukopenia Virus.The antibody titers of ascites for the hybridoma lines were 1∶12 800.The subtype of monoclonal antibodies was determined as IgG2a.Indirect immunofluorescent test indicated that the monoclonal antibody could specifically react with FPV,and it may be used as an valuable tools for diagnosis of FPV.
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development and application of spa elisa for detecting antibodies against Feline Panleukopenia Virus from two species of felids
Chinese journal of veterinary science, 2010Co-Authors: Tian Lihong, Hua YupingAbstract:Based on the purified recombinant VP2 protein of tiger Feline Panleukopenia Virus,an ELISA using SPA as conjugate(SPA-ELISA) was developed to detect FPV antibodies in clinical samples of cats and tigers.The assay was optimized that antigen coating concentration was 2.67 mg/L and a serum dilution was 1∶50,with a standard incubation time of 60 min.Blocking concentration of blocking reagent(skim milk) was 15%,with a standard incubation time of 90 min.HRP-SPA dilution was 1∶4 000,with a standard incubation time of 45 min.variation coefficient of intraassay and interassay about SPA-ELISA were all less than 10%.The contrast detection of the 30 serum samples of cats and 86 serum samples of tigers showed that the detection rate by SPA-ELISA was higher than HI,and close to indirect ELISA.The total coincidence rate of the three detecting methods was 96.7% by detecting 30 serum samples of cats.The coincidence rate of the SPA-ELISA and HI was 94.2% by detecting 86 serum samples of tigers.All these results indicated that SPA-ELISA could be a good method for serological detection of FPV antibodies in many species of Felines.
