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Jianchu Chen - One of the best experts on this subject based on the ideXlab platform.

  • composition of major Flavanone Glycosides and antioxidant capacity of three citrus varieties
    Journal of Food Biochemistry, 2009
    Co-Authors: Donghong Liu, Jianchu Chen, John Shi
    Abstract:

    This paper reported on the composition of major Flavanone Glycosides (FGs) and antioxidant capacity of three Chinese citrus varieties during maturity, namely, Ponkan (P; citrus poonensis Hort. ex Tanaka), Satsuma mandarin (S; citrus unshiu Marc.) and Huyou (H; citrus paradisi Macf. cv. Changshanhuyou). The results showed that citrus peel is a rich source of FGs. Total penolics contents (TPC) of methanol extract of P, S and H were determined. The total antioxidant capacity (TAC) of P, S and H extracts was compared based on vitamin C equivalent antioxidant capacity by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH·), 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS·+), reducing power and ferric reducing antioxidant power (FRAP) assay. Phenolic compounds may contribute a lot to TAC of fresh fruits, especially for unripe fruits. The correlations among TPC, DPPH·, ABTS·+, reducing power and FRAP assay were rather high (P < 0.05). PRACTICAL APPLICATION Citrus flavonoids are abundant in citrus fruits, especially FGs, such as hesperidin and naringin; and they are attracting more attentions for their biological properties and health benefits. The sun-dried citrus peels (chenpi) are usually used in the traditional Chinese medicine for high content of hesperidin. Investigation on the composition of FGs in peel and edible part of citrus fruits during maturity is necessary to establish a database for the future use in health foods and ingredients. Meanwhile, four methods were employed simultaneously to achieve a comprehensive evaluation on the antioxidant capacity of the three citrus varieties. The high antioxidant potential of the three selected citrus varieties made them desirable for dietary prevention of cardiovascular and other diseases.

  • phenolic compounds and antioxidant activity of extracts from ultrasonic treatment of satsuma mandarin citrus unshiu marc peels
    Journal of Agricultural and Food Chemistry, 2008
    Co-Authors: Xingqian Ye, Jianchu Chen, Zhongxiang Fang, Guihua Xu
    Abstract:

    Ultrasound-assisted extraction (UAE) was used to extract phenolic compounds from Satsuma mandarin (Citrus unshiu Marc.) peels (SMP), and maceration extraction (ME) was used as a control. The effects of ultrasonic time (10, 20, 30, 40, 50, and 60 min), temperature (15, 30, and 40 °C), and ultrasonic power (3.2, 8, 30, and 56 W) on phenolic compounds were investigated. High-performance liquid chromatography (HPLC) coupled with a photodiode array (PDA) detector was used for the analysis of phenolic acids after alkaline hydrolysis (bound phenolic acids) and Flavanone Glycosides. The contents of seven phenolic acids (caffeic acid, p-coumaric acid, ferulic acid, sinapic acid, protocatechuic acid, p-hydroxybenzoic acid, and vanillic acid) and two Flavanone Glycosides (narirutin and hesperidin) in extracts obtained by ultrasonic treatment were significantly higher than in extracts obtained by the maceration method. Moreover, the contents of extracts increased as both treatment time and temperature increased. Ultr...

  • phenolic compounds and antioxidant activity of extracts from ultrasonic treatment of satsuma mandarin citrus unshiu marc peels
    Journal of Agricultural and Food Chemistry, 2008
    Co-Authors: Zhongxiang Fang, Jianchu Chen, Donghong Liu
    Abstract:

    Ultrasound-assisted extraction (UAE) was used to extract phenolic compounds from Satsuma mandarin ( Citrus unshiu Marc.) peels (SMP), and maceration extraction (ME) was used as a control. The effects of ultrasonic time (10, 20, 30, 40, 50, and 60 min), temperature (15, 30, and 40 degrees C), and ultrasonic power (3.2, 8, 30, and 56 W) on phenolic compounds were investigated. High-performance liquid chromatography (HPLC) coupled with a photodiode array (PDA) detector was used for the analysis of phenolic acids after alkaline hydrolysis (bound phenolic acids) and Flavanone Glycosides. The contents of seven phenolic acids (caffeic acid, p-coumaric acid, ferulic acid, sinapic acid, protocatechuic acid, p-hydroxybenzoic acid, and vanillic acid) and two Flavanone Glycosides (narirutin and hesperidin) in extracts obtained by ultrasonic treatment were significantly higher than in extracts obtained by the maceration method. Moreover, the contents of extracts increased as both treatment time and temperature increased. Ultrasonic power had a positive effect on the contents of extracts. However, the phenolic acids may be degraded by ultrasound at higher temperature for a long time. For example, after ultrasonic treatment at 40 degrees C for 20 min, the contents of caffeic acid, p-coumaric acid, ferulic acid, and p-hydroxybenzoic acid decreased by 48.90, 44.20, 48.23, and 35.33%, respectively. The interaction of ultrasonic parameters probably has a complex effect on the extracts. A linear relationship was observed between Trolox equivalent antioxidant capacity (TEAC) values and total phenolic contents (TPC); the correlation coefficient, R(2), is 0.8288 at 15 degrees C, 0.7706 at 30 degrees C, and 0.8626 at 40 degrees C, respectively. The data indicated that SMPs were rich sources of antioxidants. Furthermore, UAE techniques should be carefully used to enhance the yields of phenolic acids from SMPs.

  • juice components and antioxidant capacity of citrus varieties cultivated in china
    Food Chemistry, 2008
    Co-Authors: Guihua Xu, Jianchu Chen, Xingqian Ye
    Abstract:

    Juices from fifteen citrus varieties (seven mandarins, four sweet oranges, one lemon, one grapefruit, and two pummeloes) of China were investigated mainly on quality parameters, total carotenoid, phenolic compounds (total phenolics, Flavanone Glycosides (FGs), and phenolic acids), and antioxidant capacity (ferric reducing antioxidant power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay). Among the fifteen varieties, Bendizao had the highest content of total carotenoid (10.02 mg/L), Satsuma had the highest content of narirutin (288.12 mg/L), Yinzaocheng had the highest content of hesperidin (533.64 mg/L), and Huyou had the highest content of naringin (348.53 mg/L), neohesperidin (265.25 mg/L) and total FGs (746.08 mg/L). As for total phenolic acids, Liubencheng had the highest content (72.61 mg/L). Hybrid 439 achieved the highest AA content (631.25 mg/L), and the highest total phenolics (1555.49 mg/L) and the greatest inhibition of DPPH radical (61.62%). Hamlin had the highest ascorbic acid equivalent antioxidant capacity (AEAC: 899.31 mg/L) determined by FRAP assay. Correlation coefficients of AA, total phenolics (gallic acid equivalent), FRAP (AEAC), DPPH (I%), total FGs and total phenolic acids indicated that AA played a major role for the antioxidant capacity of citrus juices, and phenolics also played an important role, which may be mainly ascribed to FGs, whilst phenolic acids seemed to play a minimal role. Furthermore, Huyou and Hybrid 439 were considered two valuable varieties from the view of antioxidant capacity and nutrition.

  • juice components and antioxidant capacity of citrus varieties cultivated in china
    Food Chemistry, 2008
    Co-Authors: Donghong Liu, Jianchu Chen, John Shi
    Abstract:

    Juices from fifteen citrus varieties (seven mandarins, four sweet oranges, one lemon, one grapefruit, and two pummeloes) of China were investigated mainly on quality parameters, total carotenoid, phenolic compounds (total phenolics, Flavanone Glycosides (FGs), and phenolic acids), and antioxidant capacity (ferric reducing antioxidant power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay). Among the fifteen varieties, Bendizao had the highest content of total carotenoid (10.02 mg/L), Satsuma had the highest content of narirutin (288.12 mg/L), Yinzaocheng had the highest content of hesperidin (533.64 mg/L), and Huyou had the highest content of naringin (348.53 mg/L), neohesperidin (265.25 mg/L) and total FGs (746.08 mg/L). As for total phenolic acids, Liubencheng had the highest content (72.61 mg/L). Hybrid 439 achieved the highest AA content (631.25 mg/L), and the highest total phenolics (1555.49 mg/L) and the greatest inhibition of DPPH radical (61.62%). Hamlin had the highest ascorbic acid equivalent antioxidant capacity (AEAC: 899.31 mg/L) determined by FRAP assay. Correlation coefficients of AA, total phenolics (gallic acid equivalent), FRAP (AEAC), DPPH (I%), total FGs and total phenolic acids indicated that AA played a major role for the antioxidant capacity of citrus juices, and phenolics also played an important role, which may be mainly ascribed to FGs, whilst phenolic acids seemed to play a minimal role. Furthermore, Huyou and Hybrid 439 were considered two valuable varieties from the view of antioxidant capacity and nutrition.

Donghong Liu - One of the best experts on this subject based on the ideXlab platform.

  • composition of major Flavanone Glycosides and antioxidant capacity of three citrus varieties
    Journal of Food Biochemistry, 2009
    Co-Authors: Donghong Liu, Jianchu Chen, John Shi
    Abstract:

    This paper reported on the composition of major Flavanone Glycosides (FGs) and antioxidant capacity of three Chinese citrus varieties during maturity, namely, Ponkan (P; citrus poonensis Hort. ex Tanaka), Satsuma mandarin (S; citrus unshiu Marc.) and Huyou (H; citrus paradisi Macf. cv. Changshanhuyou). The results showed that citrus peel is a rich source of FGs. Total penolics contents (TPC) of methanol extract of P, S and H were determined. The total antioxidant capacity (TAC) of P, S and H extracts was compared based on vitamin C equivalent antioxidant capacity by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH·), 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS·+), reducing power and ferric reducing antioxidant power (FRAP) assay. Phenolic compounds may contribute a lot to TAC of fresh fruits, especially for unripe fruits. The correlations among TPC, DPPH·, ABTS·+, reducing power and FRAP assay were rather high (P < 0.05). PRACTICAL APPLICATION Citrus flavonoids are abundant in citrus fruits, especially FGs, such as hesperidin and naringin; and they are attracting more attentions for their biological properties and health benefits. The sun-dried citrus peels (chenpi) are usually used in the traditional Chinese medicine for high content of hesperidin. Investigation on the composition of FGs in peel and edible part of citrus fruits during maturity is necessary to establish a database for the future use in health foods and ingredients. Meanwhile, four methods were employed simultaneously to achieve a comprehensive evaluation on the antioxidant capacity of the three citrus varieties. The high antioxidant potential of the three selected citrus varieties made them desirable for dietary prevention of cardiovascular and other diseases.

  • phenolic compounds and antioxidant activity of extracts from ultrasonic treatment of satsuma mandarin citrus unshiu marc peels
    Journal of Agricultural and Food Chemistry, 2008
    Co-Authors: Zhongxiang Fang, Jianchu Chen, Donghong Liu
    Abstract:

    Ultrasound-assisted extraction (UAE) was used to extract phenolic compounds from Satsuma mandarin ( Citrus unshiu Marc.) peels (SMP), and maceration extraction (ME) was used as a control. The effects of ultrasonic time (10, 20, 30, 40, 50, and 60 min), temperature (15, 30, and 40 degrees C), and ultrasonic power (3.2, 8, 30, and 56 W) on phenolic compounds were investigated. High-performance liquid chromatography (HPLC) coupled with a photodiode array (PDA) detector was used for the analysis of phenolic acids after alkaline hydrolysis (bound phenolic acids) and Flavanone Glycosides. The contents of seven phenolic acids (caffeic acid, p-coumaric acid, ferulic acid, sinapic acid, protocatechuic acid, p-hydroxybenzoic acid, and vanillic acid) and two Flavanone Glycosides (narirutin and hesperidin) in extracts obtained by ultrasonic treatment were significantly higher than in extracts obtained by the maceration method. Moreover, the contents of extracts increased as both treatment time and temperature increased. Ultrasonic power had a positive effect on the contents of extracts. However, the phenolic acids may be degraded by ultrasound at higher temperature for a long time. For example, after ultrasonic treatment at 40 degrees C for 20 min, the contents of caffeic acid, p-coumaric acid, ferulic acid, and p-hydroxybenzoic acid decreased by 48.90, 44.20, 48.23, and 35.33%, respectively. The interaction of ultrasonic parameters probably has a complex effect on the extracts. A linear relationship was observed between Trolox equivalent antioxidant capacity (TEAC) values and total phenolic contents (TPC); the correlation coefficient, R(2), is 0.8288 at 15 degrees C, 0.7706 at 30 degrees C, and 0.8626 at 40 degrees C, respectively. The data indicated that SMPs were rich sources of antioxidants. Furthermore, UAE techniques should be carefully used to enhance the yields of phenolic acids from SMPs.

  • juice components and antioxidant capacity of citrus varieties cultivated in china
    Food Chemistry, 2008
    Co-Authors: Donghong Liu, Jianchu Chen, John Shi
    Abstract:

    Juices from fifteen citrus varieties (seven mandarins, four sweet oranges, one lemon, one grapefruit, and two pummeloes) of China were investigated mainly on quality parameters, total carotenoid, phenolic compounds (total phenolics, Flavanone Glycosides (FGs), and phenolic acids), and antioxidant capacity (ferric reducing antioxidant power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay). Among the fifteen varieties, Bendizao had the highest content of total carotenoid (10.02 mg/L), Satsuma had the highest content of narirutin (288.12 mg/L), Yinzaocheng had the highest content of hesperidin (533.64 mg/L), and Huyou had the highest content of naringin (348.53 mg/L), neohesperidin (265.25 mg/L) and total FGs (746.08 mg/L). As for total phenolic acids, Liubencheng had the highest content (72.61 mg/L). Hybrid 439 achieved the highest AA content (631.25 mg/L), and the highest total phenolics (1555.49 mg/L) and the greatest inhibition of DPPH radical (61.62%). Hamlin had the highest ascorbic acid equivalent antioxidant capacity (AEAC: 899.31 mg/L) determined by FRAP assay. Correlation coefficients of AA, total phenolics (gallic acid equivalent), FRAP (AEAC), DPPH (I%), total FGs and total phenolic acids indicated that AA played a major role for the antioxidant capacity of citrus juices, and phenolics also played an important role, which may be mainly ascribed to FGs, whilst phenolic acids seemed to play a minimal role. Furthermore, Huyou and Hybrid 439 were considered two valuable varieties from the view of antioxidant capacity and nutrition.

  • minerals phenolic compounds and antioxidant capacity of citrus peel extract by hot water
    Journal of Food Science, 2007
    Co-Authors: Jianchu Chen, Donghong Liu, Yuhuan Zhang, Ping Jiang
    Abstract:

    Some dried citrus peels, more familiar as chenpi in China, have been widely used in traditional Chinese medicines from ancient times. This paper reports the efficiency of infusion cooking on extracting minerals and phenolic compounds (Flavanone Glycosides [FGs], polymethoxylated flavones [PMFs], and phenolic acids), and also antioxidant activity of hot water extract of citrus peels. Peels of 2 citrus varieties, namely, Satsuma mandarin (C. unshiu Marc.) and Ponkan (C. poonensis Hort. ex Tanaka), which belong to C. reticulata, were selected. As a result, hot water extraction was efficient in extracting phenolic acids and some minerals. As for citrus flavonoids, narirutin, nobiletin, and tangeretin were easier to extract than hesperidin. The result of antioxidant capacity assays indicated that for citrus peels, hot water extract had almost the same capacity as the methanol extract. We suggested that Ponkan was more suitable as the source of chenpi, since its hot water extract had much higher content of phenolic acids, FGs and PMFs, and higher antioxidant capacity than those of Satsuma mandarin. Generally, to raise the extraction temperature or to prolong the time could not yield higher content of phenolic compounds and stronger antioxidant capacity, though the content of minerals increased to some extent. Furthermore, a 2nd-time extraction seemed necessary since considerable minerals and phenolic compounds could be obtained by doing so. Finally, we suggested that 2 times extraction at 100 degrees C for 30 min was proper to extract the minerals and phenolic compounds in chenpi.

  • effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract
    Journal of Agricultural and Food Chemistry, 2007
    Co-Authors: Jianchu Chen, Donghong Liu
    Abstract:

    This paper reports the effects of heat treatment on huyou (Citrus paradisi Changshanhuyou) peel in terms of phenolic compounds and antioxidant capacity. High-performance liquid chromatography (HPLC) coupled with a photodiode array (PDA) detector was used in this study for the analysis of phenolic acids (divided into four fractions: free, ester, glycoside, and ester-bound) and Flavanone Glycosides (FGs) in huyou peel (HP) before and after heat treatment. The results showed that after heat treatment, the free fraction of phenolic acids increased, whereas ester, glycoside, and ester-bound fractions decreased and the content of total FGs declined (P < 0.05). Furthermore, the antioxidant activity of methanol extract of HP increased (P < 0.05), which was evaluated by total phenolics contents (TPC) assay, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS*+) method, and ferric reducing antioxidant power (FRAP) assay. The correlation coefficients among TPC, ABTS, FRAP assay, and total cinnamics and benzoics (TCB) in the free fraction were significantly high (P < 0.05), which meant that the increase of total antioxidant capacity (TAC) of HP extract was due at least in part to the increase of TCB in free fraction. In addition, FGs may be destroyed when heated at higher temperature for a long time (for example, 120 degrees C for 90 min or 150 degrees C for 30 min). Therefore, it is suggested that a proper and reasonable heat treatment could be used to enhance the antioxidant capacity of citrus peel.

Guihua Xu - One of the best experts on this subject based on the ideXlab platform.

  • phenolic compounds and antioxidant activity of extracts from ultrasonic treatment of satsuma mandarin citrus unshiu marc peels
    Journal of Agricultural and Food Chemistry, 2008
    Co-Authors: Xingqian Ye, Jianchu Chen, Zhongxiang Fang, Guihua Xu
    Abstract:

    Ultrasound-assisted extraction (UAE) was used to extract phenolic compounds from Satsuma mandarin (Citrus unshiu Marc.) peels (SMP), and maceration extraction (ME) was used as a control. The effects of ultrasonic time (10, 20, 30, 40, 50, and 60 min), temperature (15, 30, and 40 °C), and ultrasonic power (3.2, 8, 30, and 56 W) on phenolic compounds were investigated. High-performance liquid chromatography (HPLC) coupled with a photodiode array (PDA) detector was used for the analysis of phenolic acids after alkaline hydrolysis (bound phenolic acids) and Flavanone Glycosides. The contents of seven phenolic acids (caffeic acid, p-coumaric acid, ferulic acid, sinapic acid, protocatechuic acid, p-hydroxybenzoic acid, and vanillic acid) and two Flavanone Glycosides (narirutin and hesperidin) in extracts obtained by ultrasonic treatment were significantly higher than in extracts obtained by the maceration method. Moreover, the contents of extracts increased as both treatment time and temperature increased. Ultr...

  • juice components and antioxidant capacity of citrus varieties cultivated in china
    Food Chemistry, 2008
    Co-Authors: Guihua Xu, Jianchu Chen, Xingqian Ye
    Abstract:

    Juices from fifteen citrus varieties (seven mandarins, four sweet oranges, one lemon, one grapefruit, and two pummeloes) of China were investigated mainly on quality parameters, total carotenoid, phenolic compounds (total phenolics, Flavanone Glycosides (FGs), and phenolic acids), and antioxidant capacity (ferric reducing antioxidant power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay). Among the fifteen varieties, Bendizao had the highest content of total carotenoid (10.02 mg/L), Satsuma had the highest content of narirutin (288.12 mg/L), Yinzaocheng had the highest content of hesperidin (533.64 mg/L), and Huyou had the highest content of naringin (348.53 mg/L), neohesperidin (265.25 mg/L) and total FGs (746.08 mg/L). As for total phenolic acids, Liubencheng had the highest content (72.61 mg/L). Hybrid 439 achieved the highest AA content (631.25 mg/L), and the highest total phenolics (1555.49 mg/L) and the greatest inhibition of DPPH radical (61.62%). Hamlin had the highest ascorbic acid equivalent antioxidant capacity (AEAC: 899.31 mg/L) determined by FRAP assay. Correlation coefficients of AA, total phenolics (gallic acid equivalent), FRAP (AEAC), DPPH (I%), total FGs and total phenolic acids indicated that AA played a major role for the antioxidant capacity of citrus juices, and phenolics also played an important role, which may be mainly ascribed to FGs, whilst phenolic acids seemed to play a minimal role. Furthermore, Huyou and Hybrid 439 were considered two valuable varieties from the view of antioxidant capacity and nutrition.

  • effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract
    Journal of Agricultural and Food Chemistry, 2007
    Co-Authors: Guihua Xu, Xingqian Ye, Jianchu Chen
    Abstract:

    This paper reports the effects of heat treatment on huyou (Citrus paradisi Changshanhuyou) peel in terms of phenolic compounds and antioxidant capacity. High-performance liquid chromatography (HPLC) coupled with a photodiode array (PDA) detector was used in this study for the analysis of phenolic acids (divided into four fractions:  free, ester, glycoside, and ester-bound) and Flavanone Glycosides (FGs) in huyou peel (HP) before and after heat treatment. The results showed that after heat treatment, the free fraction of phenolic acids increased, whereas ester, glycoside, and ester-bound fractions decreased and the content of total FGs declined (P < 0.05). Furthermore, the antioxidant activity of methanol extract of HP increased (P < 0.05), which was evaluated by total phenolics contents (TPC) assay, 2,2‘-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) method, and ferric reducing antioxidant power (FRAP) assay. The correlation coefficients among TPC, ABTS, FRAP assay, and total cinnamics and benzoics...

Zeineb Aturki - One of the best experts on this subject based on the ideXlab platform.

  • stereoisomer separation of Flavanones and Flavanone 7 o Glycosides by means of nanoliquid chromatography employing derivatized β cyclodextrins as mobile phase additive
    Journal of Separation Science, 2020
    Co-Authors: Renata Zatopkova, Zeineb Aturki, Petr Bednař
    Abstract:

    A nanoliquid chromatographic method for the stereoisomer separation of some Flavanone aglycones and 7-O-Glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin. The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin. The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated. At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively. Precision, linearity, and sensitivity of the method were tested. Limits of detection and quantification for the studied Flavanone Glycosides were in the range 1.3-2.5 and 7.5-12.5 µg/mL, respectively. The method was used for the determination of the diastereomeric composition of the Flavanone-7-O-Glycosides in Citrus juices after solid-phase extraction procedure.

  • ordered mesoporous silica functionalized with β cyclodextrin derivative for stereoisomer separation of Flavanones and Flavanone Glycosides by nano liquid chromatography and capillary electrochromatography
    Journal of Chromatography A, 2017
    Co-Authors: Mariana Silva, Zeineb Aturki, Damian Perezquintanilla, Sonia Morantezarcero, María Luisa Marina, Isabel Sierra, Salvatore Fanali
    Abstract:

    Abstract In this paper a chiral stationary phase (CSP) was prepared by the immobilization of a β-CD derivative (3,5-dimethylphenylcarbamoylated β-CD) onto the surface of amino-functionalized spherical ordered mesoporous silica (denoted as SM) via a urea linkage using the Staudinger reaction. The CSP was packed into fused silica capillaries 100 μm I.D. and evaluated by means of nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC) using model compounds for the enantio- and the diastereomeric separation. The compounds Flavanone, 2′-hydroxyFlavanone, 4′-hydroxyFlavanone, 6-hydroxyFlavanone, 4′-methoxyFlavanone, 7-methoxyFlavanone, hesperetin, hesperidin, naringenin, and naringin were studied using reversed and polar organic elution modes. Baseline stereoisomer resolution and good results in terms of peak efficiency and short analysis time of all studied flavonoids and Flavanones Glycosides were achieved in reversed phase mode, using as mobile phase a mixture of MeOH/H2O, 10 mM ammonium acetate pH 4.5 at different ratios. For the polar organic mode using 100% of MeOH as mobile phase, the CSP showed better performances and the baseline chiral separation of several studied compounds occurred in an analysis time of less than 10 min. Good results were also achieved by CEC employing two different mobile phases. The use of MeOH/H2O, 5 mM ammonium acetate buffer pH 6.0 (90/10, v/v) was very effective for the chiral resolution of Flavanone and its methoxy and hydroxy derivatives.

  • optical isomer separation of Flavanones and Flavanone Glycosides by nano liquid chromatography using a phenyl carbamate propyl β cyclodextrin chiral stationary phase
    Journal of Chromatography A, 2010
    Co-Authors: Kahina Siahmed, Fairouz Tazerouti, Ahmed Yacine Badjahhadjahmed, Zeineb Aturki, Giovanni Dorazio, Anna Rocco, Salvatore Fanali
    Abstract:

    In this paper a phenyl-carbamate-propyl-β-cyclodextrin stationary phase was employed for the enantioseparation of several flavonoids, including Flavanones and methoxyFlavanones by using nano-liquid chromatography (nano-LC). The same stationary phase was also used for the diastereoisomeric separation of two Flavanone Glycosides. The compounds: Flavanone, 2′-hydroxyFlavanone, 4′-hydroxyFlavanone, 6-hydroxyFlavanone, 7-hydroxyFlavanone, 4′-methoxyFlavanone, 6-methoxyFlavanone, 7-methoxyFlavanone, hesperetin, hesperidin, naringenin and naringin were studied using reversed, polar organic and normal elution modes. The effect of the nature and composition of the mobile phase (organic modifier type, buffer and water content in the reversed phase mode) on the enantioresolution (Rs), retention factor (k) and enantioselectivity (α) were investigated. Baseline resolution of all studied flavonoids, with the exception of 2′-hydroxyFlavanone and naringin, was achieved in reversed phase mode using a mixture of MeOH/H2O at different ratios as mobile phase. Good results, in terms of peak efficiency and short analysis time, were obtained adding 1% triethylammonium acetate pH 4.5 buffer to MeOH/H2O mixture. The separation of the studied compounds was also performed in polar organic mode. By using 100% of MeOH as mobile phase, the resolution was achieved for the studied analytes, except for 7-hydroxyFlavanone, 2′-hydroxyFlavanone, naringenin, hesperidin and naringin. Normal mode was tested employing a mixture of EtOH/hexane/TFA as mobile phase achieving the enantiomeric and diastereomeric separation of only hesperetin and hesperidin, respectively. The use of nano-LC technique for the resolution of Flavanones optical isomers allowed to achieve good resolutions in shorter analysis time compared to the results reported in literature with conventional HPLC.

  • optical isomer separation of Flavanones and Flavanone Glycosides by nano liquid chromatography using a phenyl carbamate propyl β cyclodextrin chiral stationary phase
    Journal of Chromatography A, 2010
    Co-Authors: Kahina Siahmed, Fairouz Tazerouti, Ahmed Yacine Badjahhadjahmed, Zeineb Aturki, Giovanni Dorazio, Anna Rocco, Salvatore Fanali
    Abstract:

    In this paper a phenyl-carbamate-propyl-beta-cyclodextrin stationary phase was employed for the enantioseparation of several flavonoids, including Flavanones and methoxyFlavanones by using nano-liquid chromatography (nano-LC). The same stationary phase was also used for the diastereoisomeric separation of two Flavanone Glycosides. The compounds: Flavanone, 2'-hydroxyFlavanone, 4'-hydroxyFlavanone, 6-hydroxyFlavanone, 7-hydroxyFlavanone, 4'-methoxyFlavanone, 6-methoxyFlavanone, 7-methoxyFlavanone, hesperetin, hesperidin, naringenin and naringin were studied using reversed, polar organic and normal elution modes. The effect of the nature and composition of the mobile phase (organic modifier type, buffer and water content in the reversed phase mode) on the enantioresolution (R(s)), retention factor (k) and enantioselectivity (alpha) were investigated. Baseline resolution of all studied flavonoids, with the exception of 2'-hydroxyFlavanone and naringin, was achieved in reversed phase mode using a mixture of MeOH/H(2)O at different ratios as mobile phase. Good results, in terms of peak efficiency and short analysis time, were obtained adding 1% triethylammonium acetate pH 4.5 buffer to MeOH/H(2)O mixture. The separation of the studied compounds was also performed in polar organic mode. By using 100% of MeOH as mobile phase, the resolution was achieved for the studied analytes, except for 7-hydroxyFlavanone, 2'-hydroxyFlavanone, naringenin, hesperidin and naringin. Normal mode was tested employing a mixture of EtOH/hexane/TFA as mobile phase achieving the enantiomeric and diastereomeric separation of only hesperetin and hesperidin, respectively. The use of nano-LC technique for the resolution of Flavanones optical isomers allowed to achieve good resolutions in shorter analysis time compared to the results reported in literature with conventional HPLC.

  • separation of Flavanone 7 o glycoside diastereomers and analysis in citrus juices by multidimensional liquid chromatography coupled with mass spectrometry
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: Zeineb Aturki, Valentina Brandi, Massimo Sinibaldi
    Abstract:

    The major Flavanone-7-O-glycoside constituents in citrus fruit juices (naringin, hesperidin, neohesperidin, narirutin, and eriocitrin) were separated as diastereomers by multidimensional liquid chromatography. The method consisted of coupling two HPLC columns:  a reversed-phase (RP18) column was used for the separation of Flavanone Glycosides, which were, then, individually switched into a carboxymethylated β-cyclodextrin (β-CD)-based column and resolved as the corresponding stereoisomers. The method was used for the full analysis of Flavanone Glycosides in fresh hand-squeezed and commercial fruit juices by combining the quantitative estimation with the diastereomeric analysis. Quantitative data were in general consistent with previously reported data in this field. CC-LC isomer analysis was carried out by coupling the β-CD column with a mass spectrometer operated with negative ion electrospray ionization (ESI-MS). The results showed that hesperidin was present in orange juices almost exclusively as the 2...

Salvatore Fanali - One of the best experts on this subject based on the ideXlab platform.

  • ordered mesoporous silica functionalized with β cyclodextrin derivative for stereoisomer separation of Flavanones and Flavanone Glycosides by nano liquid chromatography and capillary electrochromatography
    Journal of Chromatography A, 2017
    Co-Authors: Mariana Silva, Zeineb Aturki, Damian Perezquintanilla, Sonia Morantezarcero, María Luisa Marina, Isabel Sierra, Salvatore Fanali
    Abstract:

    Abstract In this paper a chiral stationary phase (CSP) was prepared by the immobilization of a β-CD derivative (3,5-dimethylphenylcarbamoylated β-CD) onto the surface of amino-functionalized spherical ordered mesoporous silica (denoted as SM) via a urea linkage using the Staudinger reaction. The CSP was packed into fused silica capillaries 100 μm I.D. and evaluated by means of nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC) using model compounds for the enantio- and the diastereomeric separation. The compounds Flavanone, 2′-hydroxyFlavanone, 4′-hydroxyFlavanone, 6-hydroxyFlavanone, 4′-methoxyFlavanone, 7-methoxyFlavanone, hesperetin, hesperidin, naringenin, and naringin were studied using reversed and polar organic elution modes. Baseline stereoisomer resolution and good results in terms of peak efficiency and short analysis time of all studied flavonoids and Flavanones Glycosides were achieved in reversed phase mode, using as mobile phase a mixture of MeOH/H2O, 10 mM ammonium acetate pH 4.5 at different ratios. For the polar organic mode using 100% of MeOH as mobile phase, the CSP showed better performances and the baseline chiral separation of several studied compounds occurred in an analysis time of less than 10 min. Good results were also achieved by CEC employing two different mobile phases. The use of MeOH/H2O, 5 mM ammonium acetate buffer pH 6.0 (90/10, v/v) was very effective for the chiral resolution of Flavanone and its methoxy and hydroxy derivatives.

  • optical isomer separation of Flavanones and Flavanone Glycosides by nano liquid chromatography using a phenyl carbamate propyl β cyclodextrin chiral stationary phase
    Journal of Chromatography A, 2010
    Co-Authors: Kahina Siahmed, Fairouz Tazerouti, Ahmed Yacine Badjahhadjahmed, Zeineb Aturki, Giovanni Dorazio, Anna Rocco, Salvatore Fanali
    Abstract:

    In this paper a phenyl-carbamate-propyl-β-cyclodextrin stationary phase was employed for the enantioseparation of several flavonoids, including Flavanones and methoxyFlavanones by using nano-liquid chromatography (nano-LC). The same stationary phase was also used for the diastereoisomeric separation of two Flavanone Glycosides. The compounds: Flavanone, 2′-hydroxyFlavanone, 4′-hydroxyFlavanone, 6-hydroxyFlavanone, 7-hydroxyFlavanone, 4′-methoxyFlavanone, 6-methoxyFlavanone, 7-methoxyFlavanone, hesperetin, hesperidin, naringenin and naringin were studied using reversed, polar organic and normal elution modes. The effect of the nature and composition of the mobile phase (organic modifier type, buffer and water content in the reversed phase mode) on the enantioresolution (Rs), retention factor (k) and enantioselectivity (α) were investigated. Baseline resolution of all studied flavonoids, with the exception of 2′-hydroxyFlavanone and naringin, was achieved in reversed phase mode using a mixture of MeOH/H2O at different ratios as mobile phase. Good results, in terms of peak efficiency and short analysis time, were obtained adding 1% triethylammonium acetate pH 4.5 buffer to MeOH/H2O mixture. The separation of the studied compounds was also performed in polar organic mode. By using 100% of MeOH as mobile phase, the resolution was achieved for the studied analytes, except for 7-hydroxyFlavanone, 2′-hydroxyFlavanone, naringenin, hesperidin and naringin. Normal mode was tested employing a mixture of EtOH/hexane/TFA as mobile phase achieving the enantiomeric and diastereomeric separation of only hesperetin and hesperidin, respectively. The use of nano-LC technique for the resolution of Flavanones optical isomers allowed to achieve good resolutions in shorter analysis time compared to the results reported in literature with conventional HPLC.

  • optical isomer separation of Flavanones and Flavanone Glycosides by nano liquid chromatography using a phenyl carbamate propyl β cyclodextrin chiral stationary phase
    Journal of Chromatography A, 2010
    Co-Authors: Kahina Siahmed, Fairouz Tazerouti, Ahmed Yacine Badjahhadjahmed, Zeineb Aturki, Giovanni Dorazio, Anna Rocco, Salvatore Fanali
    Abstract:

    In this paper a phenyl-carbamate-propyl-beta-cyclodextrin stationary phase was employed for the enantioseparation of several flavonoids, including Flavanones and methoxyFlavanones by using nano-liquid chromatography (nano-LC). The same stationary phase was also used for the diastereoisomeric separation of two Flavanone Glycosides. The compounds: Flavanone, 2'-hydroxyFlavanone, 4'-hydroxyFlavanone, 6-hydroxyFlavanone, 7-hydroxyFlavanone, 4'-methoxyFlavanone, 6-methoxyFlavanone, 7-methoxyFlavanone, hesperetin, hesperidin, naringenin and naringin were studied using reversed, polar organic and normal elution modes. The effect of the nature and composition of the mobile phase (organic modifier type, buffer and water content in the reversed phase mode) on the enantioresolution (R(s)), retention factor (k) and enantioselectivity (alpha) were investigated. Baseline resolution of all studied flavonoids, with the exception of 2'-hydroxyFlavanone and naringin, was achieved in reversed phase mode using a mixture of MeOH/H(2)O at different ratios as mobile phase. Good results, in terms of peak efficiency and short analysis time, were obtained adding 1% triethylammonium acetate pH 4.5 buffer to MeOH/H(2)O mixture. The separation of the studied compounds was also performed in polar organic mode. By using 100% of MeOH as mobile phase, the resolution was achieved for the studied analytes, except for 7-hydroxyFlavanone, 2'-hydroxyFlavanone, naringenin, hesperidin and naringin. Normal mode was tested employing a mixture of EtOH/hexane/TFA as mobile phase achieving the enantiomeric and diastereomeric separation of only hesperetin and hesperidin, respectively. The use of nano-LC technique for the resolution of Flavanones optical isomers allowed to achieve good resolutions in shorter analysis time compared to the results reported in literature with conventional HPLC.