The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Denis-claude Roy - One of the best experts on this subject based on the ideXlab platform.
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Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in Fluorinated Ethylene Propylene cell culture bags.
Journal of Translational Medicine, 2020Co-Authors: Jean-philippe Bastien, Natalie Fekete, Ariane V. Beland, Marie-paule Lachambre, Veronique Laforte, David Juncker, Vibhuti Dave, Denis-claude Roy, Corinne A. HoesliAbstract:BACKGROUND A major obstacle to anti-viral and -tumor cell vaccination and T cell immunotherapy is the ability to produce dendritic cells (DCs) in a suitable clinical setting. It is imperative to develop closed cell culture systems to accelerate the translation of promising DC-based cell therapy products to the clinic. The objective of this study was to investigate whether viral antigen-loaded monocyte-derived DCs (Mo-DCs) capable of eliciting specific T cell activation can be manufactured in Fluorinated Ethylene Propylene (FEP) bags. METHODS Mo-DCs were generated through a protocol applying cytokine cocktails combined with lipopolysaccharide or with a CMV viral peptide antigen in conventional tissue culture polystyrene (TCPS) or FEP culture vessels. Research-scale (
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closing the system production of viral antigen presenting dendritic cells eliciting specific cd8 t cell activation in Fluorinated Ethylene Propylene cell culture bags
Journal of Translational Medicine, 2020Co-Authors: Jean-philippe Bastien, Natalie Fekete, Ariane V. Beland, Marie-paule Lachambre, Veronique Laforte, David Juncker, Vibhuti Dave, Denis-claude RoyAbstract:BACKGROUND A major obstacle to anti-viral and -tumor cell vaccination and T cell immunotherapy is the ability to produce dendritic cells (DCs) in a suitable clinical setting. It is imperative to develop closed cell culture systems to accelerate the translation of promising DC-based cell therapy products to the clinic. The objective of this study was to investigate whether viral antigen-loaded monocyte-derived DCs (Mo-DCs) capable of eliciting specific T cell activation can be manufactured in Fluorinated Ethylene Propylene (FEP) bags. METHODS Mo-DCs were generated through a protocol applying cytokine cocktails combined with lipopolysaccharide or with a CMV viral peptide antigen in conventional tissue culture polystyrene (TCPS) or FEP culture vessels. Research-scale (< 10 mL) FEP bags were implemented to increase R&D throughput. DC surface marker profiles, cytokine production, and ability to activate antigen-specific cytotoxic T cells were characterized. RESULTS Monocyte differentiation into Mo-DCs led to the loss of CD14 expression with concomitant upregulation of CD80, CD83 and CD86. Significantly increased levels of IL-10 and IL-12 were observed after maturation on day 9. Antigen-pulsed Mo-DCs activated antigen-responsive CD8+ cytotoxic T cells. No significant differences in surface marker expression or tetramer-specific T cell activating potency of Mo-DCs were observed between TCPS and FEP culture vessels. CONCLUSIONS Our findings demonstrate that viral antigen-loaded Mo-DCs produced in downscaled FEP bags can elicit specific T cell responses. In view of the dire clinical need for closed system DC manufacturing, FEP bags represent an attractive option to accelerate the translation of promising emerging DC-based immunotherapies.
Ranty H. Liang - One of the best experts on this subject based on the ideXlab platform.
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Vacuum ultraviolet radiation/atomic oxygen synergism in Fluorinated Ethylene Propylene Teflon erosion
Journal of Spacecraft and Rockets, 1992Co-Authors: Albert E. Stiegman, David E. Brinza, Eric G. Laue, Mark S. Anderson, Ranty H. LiangAbstract:A micrographic investigation is reported of samples of the Fluorinated Ethylene Propylene (FEP) Teflon thermal-blanketing materials recovered from the Long-Duration Exposure Facility (LDEF) satellite. The samples are taken from the trailing edge and row 8 which correspond to exposures to vacuum UV (VUV) and VUV + atomic O, respectively. Data are taken from SEM and IR-spectra observations, and the LDEF leading-edge FEP shows a high degree of erosion, roughening, and sharp peaks angled in the direction of the flow of atomic O. The trailing edge sample influenced primarily by VUV shows a hard brittle layer and some cracked mosaic patterns. Comparisons to a reference sample suggest that the brittle layer is related to exposure to VUV and is removed by atomic-O impingement. Polymers that are stable to VUV radiation appear to be more stable in terms of atomic oxygen.
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vacuum ultraviolet radiation atomic oxygen synergism in Fluorinated Ethylene Propylene teflon erosion
Journal of Spacecraft and Rockets, 1992Co-Authors: Albert E. Stiegman, David E. Brinza, Eric G. Laue, Mark S. Anderson, Ranty H. LiangAbstract:A micrographic investigation is reported of samples of the Fluorinated Ethylene Propylene (FEP) Teflon thermal-blanketing materials recovered from the Long-Duration Exposure Facility (LDEF) satellite. The samples are taken from the trailing edge and row 8 which correspond to exposures to vacuum UV (VUV) and VUV + atomic O, respectively. Data are taken from SEM and IR-spectra observations, and the LDEF leading-edge FEP shows a high degree of erosion, roughening, and sharp peaks angled in the direction of the flow of atomic O. The trailing edge sample influenced primarily by VUV shows a hard brittle layer and some cracked mosaic patterns. Comparisons to a reference sample suggest that the brittle layer is related to exposure to VUV and is removed by atomic-O impingement. Polymers that are stable to VUV radiation appear to be more stable in terms of atomic oxygen.
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Vacuum Ultraviolet (VUV) radiation-induced degradation of Fluorinated Ethylene Propylene (FEP) Teflon aboard the Long Duration Exposure Facility (LDEF)
1992Co-Authors: David E. Brinza, Albert E. Stiegman, Eric G. Laue, Paul R. Staszak, Ranty H. LiangAbstract:Examination of Fluorinated Ethylene Propylene (FEP) copolymer specimens recovered from the Long Duration Exposure Facility (LDEF) provides evidence for degradation attributed to extended solar vacuum ultraviolet (VUV) irradiation. Scanning electron microscope (SEM) images of sheared FEP film edges reveal the presence of a highly embrittled layer on the exposed surface of specimens obtained from the trailing edge of the LDEF. Similar images obtained for leading edge and control FEP films do not exhibit evidence for such an embrittled layer. Laboratory VUV irradiation of FEP films is found to produce a damage layer similar to that witnessed in the LDEF trailing edge films. Spectroscopic analyses of irradiated films provide data to advance a photochemical mechanism for degradation.
Judith Kadarusman - One of the best experts on this subject based on the ideXlab platform.
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Growing Cholesterol‐Dependent NS0 Myeloma Cell Line in the Wave Bioreactor System: Overcoming Cholesterol‐Polymer Interaction by Using Pretreated Polymer or Inert Fluorinated Ethylene Propylene
Biotechnology Progress, 2008Co-Authors: Judith Kadarusman, Ravi Bhatia, John Mclaughlin, Wenglong R. LinAbstract:Difficulty in growing cholesterol-dependent NS0 cells in the Wave bioreactor using the original low-density polyPropylene (LDPE) bags has been encountered. It has been shown that in these bags chemically defined cholesterol is depleted from solution and therefore unavailable for the cells. Our data suggest that the cause of the depletion is not chemical but is due to the physical structure of the polymer. It is proposed that polymer structures with inkbottle pores retain cholesterol, whereas structures with V-shaped pores adsorb cholesterol reversibly. Ultra-low-density polyEthylene (ULDPE) bags can support cell growth but need to be pretreated with excess cholesterol. Another material, Fluorinated Ethylene Propylene (FEP) does not need to be pretreated and is found to be superior (negligible cholesterol adsorption) as a result of its inert characteristics.
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growing cholesterol dependent ns0 myeloma cell line in the wave bioreactor system overcoming cholesterol polymer interaction by using pretreated polymer or inert Fluorinated Ethylene Propylene
Biotechnology Progress, 2005Co-Authors: Judith Kadarusman, Ravi Bhatia, John MclaughlinAbstract:Difficulty in growing cholesterol-dependent NS0 cells in the Wave bioreactor using the original low-density polyPropylene (LDPE) bags has been encountered. It has been shown that in these bags chemically defined cholesterol is depleted from solution and therefore unavailable for the cells. Our data suggest that the cause of the depletion is not chemical but is due to the physical structure of the polymer. It is proposed that polymer structures with inkbottle pores retain cholesterol, whereas structures with V-shaped pores adsorb cholesterol reversibly. Ultra-low-density polyEthylene (ULDPE) bags can support cell growth but need to be pretreated with excess cholesterol. Another material, Fluorinated Ethylene Propylene (FEP) does not need to be pretreated and is found to be superior (negligible cholesterol adsorption) as a result of its inert characteristics.
Yanji Zhu - One of the best experts on this subject based on the ideXlab platform.
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Facile preparation of superamphiphobic epoxy resin/modified poly(vinylidene fluoride)/Fluorinated Ethylene Propylene composite coating with corrosion/wear-resistance
Applied Surface Science, 2015Co-Authors: Huaiyuan Wang, Zhanjian Liu, Enqun Wang, Xiguang Zhang, Ruixia Yuan, Yanji ZhuAbstract:Abstract A robust superamphiphobic epoxy resin (EP)/modified poly(vinylidene fluoride) (MPVDF)/Fluorinated Ethylene Propylene (FEP) composite coating has been prepared through the combination of chemical modification and spraying technique. Nanometer silica (SiO 2 , 2.5 wt.%) and carbon nanotubes (CNTs, 2.5 wt.%) were added in the coating to construct the necessary reticulate papillae structures for superamphiphobic surface. The prepared EP composite coating demonstrated high static contact angles (166°, 155°) and low sliding angles (3°, 5°) to water and glycerol, respectively. Moreover, the prepared coating can also retain superhydrophobicity under strongly acidic and alkaline conditions. The brittleness of EP can be avoided by introducing the malleable MPVDF. The wear life of the EP composite coating with 25 wt.% FEP was improved to 18 times of the pure EP coating. The increased wear life of the coating can be attributed to the designed nano/micro structures, the self-lubrication of FEP and the chemical reaction between EP and MPVDF. The anti-corrosion performance of the coatings was investigated in 3.5% NaCl solution using potentiodynamic polarization. The results showed that the prepared superamphiphobic composite coating was most effective in corrosion resistance, primarily due to the barrier effect for the diffusion of O 2 and H 2 O molecules. It is believed that this robust superamphiphobic EP/MPVDF/FEP composite coating prepared by the facile spray method can pave a way for the large-scale application in pipeline transport.
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facile preparation of superamphiphobic epoxy resin modified poly vinylidene fluoride Fluorinated Ethylene Propylene composite coating with corrosion wear resistance
Applied Surface Science, 2015Co-Authors: Huaiyuan Wang, Zhanjian Liu, Enqun Wang, Xiguang Zhang, Ruixia Yuan, Yanji ZhuAbstract:Abstract A robust superamphiphobic epoxy resin (EP)/modified poly(vinylidene fluoride) (MPVDF)/Fluorinated Ethylene Propylene (FEP) composite coating has been prepared through the combination of chemical modification and spraying technique. Nanometer silica (SiO 2 , 2.5 wt.%) and carbon nanotubes (CNTs, 2.5 wt.%) were added in the coating to construct the necessary reticulate papillae structures for superamphiphobic surface. The prepared EP composite coating demonstrated high static contact angles (166°, 155°) and low sliding angles (3°, 5°) to water and glycerol, respectively. Moreover, the prepared coating can also retain superhydrophobicity under strongly acidic and alkaline conditions. The brittleness of EP can be avoided by introducing the malleable MPVDF. The wear life of the EP composite coating with 25 wt.% FEP was improved to 18 times of the pure EP coating. The increased wear life of the coating can be attributed to the designed nano/micro structures, the self-lubrication of FEP and the chemical reaction between EP and MPVDF. The anti-corrosion performance of the coatings was investigated in 3.5% NaCl solution using potentiodynamic polarization. The results showed that the prepared superamphiphobic composite coating was most effective in corrosion resistance, primarily due to the barrier effect for the diffusion of O 2 and H 2 O molecules. It is believed that this robust superamphiphobic EP/MPVDF/FEP composite coating prepared by the facile spray method can pave a way for the large-scale application in pipeline transport.
John Mclaughlin - One of the best experts on this subject based on the ideXlab platform.
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Growing Cholesterol‐Dependent NS0 Myeloma Cell Line in the Wave Bioreactor System: Overcoming Cholesterol‐Polymer Interaction by Using Pretreated Polymer or Inert Fluorinated Ethylene Propylene
Biotechnology Progress, 2008Co-Authors: Judith Kadarusman, Ravi Bhatia, John Mclaughlin, Wenglong R. LinAbstract:Difficulty in growing cholesterol-dependent NS0 cells in the Wave bioreactor using the original low-density polyPropylene (LDPE) bags has been encountered. It has been shown that in these bags chemically defined cholesterol is depleted from solution and therefore unavailable for the cells. Our data suggest that the cause of the depletion is not chemical but is due to the physical structure of the polymer. It is proposed that polymer structures with inkbottle pores retain cholesterol, whereas structures with V-shaped pores adsorb cholesterol reversibly. Ultra-low-density polyEthylene (ULDPE) bags can support cell growth but need to be pretreated with excess cholesterol. Another material, Fluorinated Ethylene Propylene (FEP) does not need to be pretreated and is found to be superior (negligible cholesterol adsorption) as a result of its inert characteristics.
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growing cholesterol dependent ns0 myeloma cell line in the wave bioreactor system overcoming cholesterol polymer interaction by using pretreated polymer or inert Fluorinated Ethylene Propylene
Biotechnology Progress, 2005Co-Authors: Judith Kadarusman, Ravi Bhatia, John MclaughlinAbstract:Difficulty in growing cholesterol-dependent NS0 cells in the Wave bioreactor using the original low-density polyPropylene (LDPE) bags has been encountered. It has been shown that in these bags chemically defined cholesterol is depleted from solution and therefore unavailable for the cells. Our data suggest that the cause of the depletion is not chemical but is due to the physical structure of the polymer. It is proposed that polymer structures with inkbottle pores retain cholesterol, whereas structures with V-shaped pores adsorb cholesterol reversibly. Ultra-low-density polyEthylene (ULDPE) bags can support cell growth but need to be pretreated with excess cholesterol. Another material, Fluorinated Ethylene Propylene (FEP) does not need to be pretreated and is found to be superior (negligible cholesterol adsorption) as a result of its inert characteristics.