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Celuta S. Alviano - One of the best experts on this subject based on the ideXlab platform.
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Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system.
Memórias do Instituto Oswaldo Cruz, 2018Co-Authors: Lysianne Pinto, Luiz Fernando Zmetek Granja, Mariana Amorim De Almeida, Daniela S. Alviano, Maria Helena Da Silva, Regina Ejzemberg, Sonia Rozental, Celuta S. AlvianoAbstract:BACKGROUND Melanin production has been associated with virulence in various pathogenic fungi, including Fonsecaea pedrosoi, the major etiological agent for chromoblastomycosis, a subcutaneous fungal disease that occurs in South America. OBJECTIVE The aim of this study was to evaluate the effects of acid-basic extracted F. pedrosoi melanin particles and fungal cell ghosts obtained by Novozym 234 treatment on their ability to activate the human complement system. METHODS The ability of melanin particles and fungal cell ghosts to activate the human complement system was evaluated by complement consumption, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). FINDINGS Unsensitised melanin particles and melanin ghosts presented complement consumption of 82.67 ± 2.08% and 96.04 ± 1.13%, respectively. Immunofluorescence assays revealed intense deposition of the C3 and C4 fragments on the surface of melanin particles and ghosts extracted from F. pedrosoi. Deposition of the C3, C4, and C5 fragments onto melanin samples and zymosan was confirmed by ELISA. Deposition of small amounts of C1q and C9 onto melanin samples and zymosan was detected by ELISA. CONCLUSION Fonsecaea pedrosoi melanin particles and fungal cell ghosts activated the complement system mainly through an alternative pathway.
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Putative role of an ABC transporter in Fonsecaea pedrosoi multidrug resistance
International Journal of Antimicrobial Agents, 2012Co-Authors: Luciana P. Rangel, Celuta S. Alviano, Daniela S. Alviano, Otacilio C. Moreira, Gustavo N. Livramento, Constança Britto, Antonio Ferreira-pereiraAbstract:Fonsecaea pedrosoi, a dematiaceous fungus, is the main agent responsible for chromoblastomycosis, a chronic and progressive mycosis of the skin and subcutaneous tissues. This disease can cause different types of lesions depending on the immune status of the host. Its treatment is complicated by the toxicity of available antifungal agents as well as drug resistance. In this work, an ATP-binding cassette (ABC) transporter in this fungus was characterised, with the degree of expression related to the drug resistance of two strains (a patient isolated strain and a laboratory strain). A 150 kDa protein was detected by western blotting. The ATPase activity of membrane preparations was also evaluated. The F. pedrosoi transporter appears to behave like Pdr5p, a well-studied multidrug resistance transporter in Saccharomyces cerevisiae with the ability to hydrolyse different triphosphate nucleotides, as well as its response to classical inhibitors tested. Finally, a reverse transcription polymerase chain reaction (RT-PCR) approach was used and a 400 bp product was detected, corresponding to the highly conserved ATP-binding domain of ABC transporters. We suggest that an ABC transporter must be involved in F. pedrosoi multidrug resistance, and a complete understanding of this protein could bring an important contribution to antifungal treatment of this disease.
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Activation of the human complement system by pigmented and hypopigmented mycelia of the fungus Fonsecaea pedrosoi.
Mycoses, 2011Co-Authors: Lysianne Pinto, Celuta S. Alviano, Luiz Fernando Zmetek Granja, Daniela S. Alviano, Maria Helena Da Silva, Regina EjzembergAbstract:Summary The action of the complement system on pigmented and hypopigmented mycelia of the fungus Fonsecaea pedrosoi, the major aetiological pathogen of the chromoblastomycosis is herein discussed. Fungi were grown in medium Czapeck-Dox at 37 °C, for 14 days, without shaking to obtain pigmented mycelium. To obtain hypopigmented mycelium, the fungus was grown at the same conditions, but in the dark and with low oxygenation. Activation was measured by complement consumption and enzyme-linked immunosorbent assay. We also observed by immunofluorescence the deposition of C3, C4 fragments and C9 on the surface of the different forms studied. The results indicate that both forms were able to activate the complement system mainly by the alternative pathway. Pigmented mycelia had the highest consumption results, indicating that the pigment, melanin, may have influence in activation.
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Phospholipase and Esterase Production by Clinical Strains of Fonsecaea pedrosoi and Their Interactions with Epithelial Cells
Mycopathologia, 2010Co-Authors: Vanila Faber Palmeira, Lucimar F. Kneipp, Celuta S. Alviano, André Luis Souza SantosAbstract:Fonsecaea pedrosoi is the major etiologic agent of chromoblastomycosis. The virulence of F. pedrosoi is a meagerly explored phenomenon. The ability to interact with host cells and the production of hydrolytic enzymes are thought to be important virulence mechanisms of fungal pathogens. Here, we measured the production of two distinct lipolytic enzymes, phospholipase and esterase, by three clinical strains of F. pedrosoi isolated from chromoblastomycosis lesions, as well as their capabilities to interact with epithelial cells. All the strains were excellent esterase producers, generating elevated hydrolytic halos after 5 days of growth. Conversely, phospholipase activity was detected only after 10 days, except for the most recent strain of F. pedrosoi (Magé) in which measurable phospholipase activity was detected on day 5. The ability to interact with epithelial cells was also investigated. Regarding the adhesion capability, an indirect connection was observed in relation to the adaptation time of each strain in axenic culture, in which Magé strain showed the best adhesion ability followed by LDI 11428 and 5VPL strains. Both 5VPL and Magé strains were also detected inside the epithelial cells, while the LDI 11428 strain was rarely detected in cytoplasmatic vacuolar compartments. Moreover, these F. pedrosoi strains were able to cause injury in epithelial cells.
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Biology and pathogenesis of Fonsecaea pedrosoi, the major etiologic agent of chromoblastomycosis.
FEMS Microbiology Reviews, 2007Co-Authors: André L.s. Santos, Lucimar F. Kneipp, Marcio L. Rodrigues, Daniela S. Alviano, Sonia Rozental, Vanila F. Palmeira, Leonardo Nimrichter, Celuta S. AlvianoAbstract:Fonsecaea pedrosoi is the principal etiologic agent of chromoblastomycosis, a fungal disease whose pathogenic events are poorly understood. Treatment of the disease presents poor effectiveness and serious side effects. The disease is epidemiologically important in several regions, which has stimulated studies focused on the biology and pathogenic potential of its major causative agent. In this review, we summarize the current knowledge on the biological aspects of F. pedrosoi, including cell differentiation and pathogenic mechanisms during the interaction of fungi with different hosts' elements.
Sonia Rozental - One of the best experts on this subject based on the ideXlab platform.
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Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system.
Memórias do Instituto Oswaldo Cruz, 2018Co-Authors: Lysianne Pinto, Luiz Fernando Zmetek Granja, Mariana Amorim De Almeida, Daniela S. Alviano, Maria Helena Da Silva, Regina Ejzemberg, Sonia Rozental, Celuta S. AlvianoAbstract:BACKGROUND Melanin production has been associated with virulence in various pathogenic fungi, including Fonsecaea pedrosoi, the major etiological agent for chromoblastomycosis, a subcutaneous fungal disease that occurs in South America. OBJECTIVE The aim of this study was to evaluate the effects of acid-basic extracted F. pedrosoi melanin particles and fungal cell ghosts obtained by Novozym 234 treatment on their ability to activate the human complement system. METHODS The ability of melanin particles and fungal cell ghosts to activate the human complement system was evaluated by complement consumption, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). FINDINGS Unsensitised melanin particles and melanin ghosts presented complement consumption of 82.67 ± 2.08% and 96.04 ± 1.13%, respectively. Immunofluorescence assays revealed intense deposition of the C3 and C4 fragments on the surface of melanin particles and ghosts extracted from F. pedrosoi. Deposition of the C3, C4, and C5 fragments onto melanin samples and zymosan was confirmed by ELISA. Deposition of small amounts of C1q and C9 onto melanin samples and zymosan was detected by ELISA. CONCLUSION Fonsecaea pedrosoi melanin particles and fungal cell ghosts activated the complement system mainly through an alternative pathway.
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Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals
BMC Microbiology, 2010Co-Authors: Marcel M.l. Cunha, Wanderley De Souza, Anderson J. Franzen, Sergio Henrique Seabra, Marcelo H. Herbst, N.v. Vugman, Luana P Borba, Sonia RozentalAbstract:Background The pathogenic fungus Fonsecaea pedrosoi constitutively produces the pigment melanin, an important virulence factor in fungi. Melanin is incorporated in the cell wall structure and provides chemical and physical protection for the fungus. We evaluated the production of nitric oxide (NO) in macrophages, the oxidative burst and the inducible nitric oxide synthase (i-NOS) activity in interactions between activated murine macrophages and F. pedrosoi. Experiments were carried out with or without tricyclazole (TC) treatment, a selective inhibitor of the dihydroxynaphthalene (DHN)-melanin biosynthesis pathway in F. pedrosoi. The paramagnetisms of melanin and the TC-melanin were analysed by electron spin resonance. The fungal growth responses to H2O2 and to S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide donor, were also evaluated.
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Ultrastructural characterization of melanosomes of the human pathogenic fungus Fonsecaea pedrosoi.
Journal of Structural Biology, 2008Co-Authors: Anderson J. Franzen, Wanderley De Souza, Moises Batista Da Silva, Claudio Guedes Salgado, Marcel M.l. Cunha, Kildare Miranda, Joachim Hentschel, Helmut Plattner, Sonia RozentalAbstract:Melanin is a complex polymer widely distributed in nature and has been described as an important virulence factor in pathogenic fungi. In the majority of fungi, the mechanism of melanin formation remains unclear. In Fonsecaea pedrosoi, the major etiologic agent of chromoblastomycosis, melanin is stored in intracellular vesicles, named melanosomes. This paper details the ultrastructural aspects of melanin formation, its storage and transportation to the cell wall in the human pathogenic fungus F. pedrosoi. In this fungus, melanin synthesis within melanosomes also begins with a fibrillar matrix formation, displaying morphological and structural features similar to melanosomes from amphibian and mammalian cells. Silver precipitation based on Fontana-Masson technique for melanin detection and immunocytochemistry showed that melanosome fuses with fungal cell membrane where the melanin is released and reaches the cell wall. Melanin deposition in the fungal cell wall occurs in concentric layers. Antibodies raised against F. pedrosoi melanin revealed the sites of melanin production and storage in the melanosomes. In addition, a preliminary description of the elemental composition of this organelle by X-ray microanalysis and elemental mapping revealed the presence of calcium, phosphorus and iron concentrated in its matrix, suggesting a new functional role for these organelles as iron storage compartments.
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Biology and pathogenesis of Fonsecaea pedrosoi, the major etiologic agent of chromoblastomycosis.
FEMS Microbiology Reviews, 2007Co-Authors: André L.s. Santos, Lucimar F. Kneipp, Marcio L. Rodrigues, Daniela S. Alviano, Sonia Rozental, Vanila F. Palmeira, Leonardo Nimrichter, Celuta S. AlvianoAbstract:Fonsecaea pedrosoi is the principal etiologic agent of chromoblastomycosis, a fungal disease whose pathogenic events are poorly understood. Treatment of the disease presents poor effectiveness and serious side effects. The disease is epidemiologically important in several regions, which has stimulated studies focused on the biology and pathogenic potential of its major causative agent. In this review, we summarize the current knowledge on the biological aspects of F. pedrosoi, including cell differentiation and pathogenic mechanisms during the interaction of fungi with different hosts' elements.
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Protein kinase antagonists inhibit invasion of mammalian cells by Fonsecaea pedrosoi.
Journal of Medical Microbiology, 2003Co-Authors: Cristiana L. Limongi, Wanderley De Souza, Sonia RozentalAbstract:The phosphorylation process is an important mechanism of cell signalling and regulation. It has been implicated recently in defence strategies against a variety of pathogens that alter host signalling pathways in order to facilitate their invasion and survival within host cells. In this study, the involvement of protein kinases (PKs) has been investigated in attachment and invasion by the pathogenic fungus Fonsecaea pedrosoi within epithelial cells and macrophages. The use of the PK inhibitors staurosporine, genistein and calphostin C prior to infection provided significant information about the role played by PKs in the F. pedrosoi–host cell interaction. All three PK inhibitors could reduce cell invasion by F. pedrosoi significantly. Pre-treatment of macrophages, epithelial cells or conidia with PK inhibitors decreased fungus invasion, and this effect could be overcome by okadaic acid, a phosphatase inhibitor. Immunofluorescence assays showed that tyrosine residues were phosphorylated in the first step of the interaction, while serine residues were phosphorylated in the subsequent step of entry of the parasite into the host cell. These results suggest that both host-cell and conidium PK activities are important in the interaction process, playing a significant role in cell invasion.
Wanderley De Souza - One of the best experts on this subject based on the ideXlab platform.
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Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals
BMC Microbiology, 2010Co-Authors: Marcel M.l. Cunha, Wanderley De Souza, Anderson J. Franzen, Sergio Henrique Seabra, Marcelo H. Herbst, N.v. Vugman, Luana P Borba, Sonia RozentalAbstract:Background The pathogenic fungus Fonsecaea pedrosoi constitutively produces the pigment melanin, an important virulence factor in fungi. Melanin is incorporated in the cell wall structure and provides chemical and physical protection for the fungus. We evaluated the production of nitric oxide (NO) in macrophages, the oxidative burst and the inducible nitric oxide synthase (i-NOS) activity in interactions between activated murine macrophages and F. pedrosoi. Experiments were carried out with or without tricyclazole (TC) treatment, a selective inhibitor of the dihydroxynaphthalene (DHN)-melanin biosynthesis pathway in F. pedrosoi. The paramagnetisms of melanin and the TC-melanin were analysed by electron spin resonance. The fungal growth responses to H2O2 and to S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide donor, were also evaluated.
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Ultrastructural characterization of melanosomes of the human pathogenic fungus Fonsecaea pedrosoi.
Journal of Structural Biology, 2008Co-Authors: Anderson J. Franzen, Wanderley De Souza, Moises Batista Da Silva, Claudio Guedes Salgado, Marcel M.l. Cunha, Kildare Miranda, Joachim Hentschel, Helmut Plattner, Sonia RozentalAbstract:Melanin is a complex polymer widely distributed in nature and has been described as an important virulence factor in pathogenic fungi. In the majority of fungi, the mechanism of melanin formation remains unclear. In Fonsecaea pedrosoi, the major etiologic agent of chromoblastomycosis, melanin is stored in intracellular vesicles, named melanosomes. This paper details the ultrastructural aspects of melanin formation, its storage and transportation to the cell wall in the human pathogenic fungus F. pedrosoi. In this fungus, melanin synthesis within melanosomes also begins with a fibrillar matrix formation, displaying morphological and structural features similar to melanosomes from amphibian and mammalian cells. Silver precipitation based on Fontana-Masson technique for melanin detection and immunocytochemistry showed that melanosome fuses with fungal cell membrane where the melanin is released and reaches the cell wall. Melanin deposition in the fungal cell wall occurs in concentric layers. Antibodies raised against F. pedrosoi melanin revealed the sites of melanin production and storage in the melanosomes. In addition, a preliminary description of the elemental composition of this organelle by X-ray microanalysis and elemental mapping revealed the presence of calcium, phosphorus and iron concentrated in its matrix, suggesting a new functional role for these organelles as iron storage compartments.
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Protein kinase antagonists inhibit invasion of mammalian cells by Fonsecaea pedrosoi.
Journal of Medical Microbiology, 2003Co-Authors: Cristiana L. Limongi, Wanderley De Souza, Sonia RozentalAbstract:The phosphorylation process is an important mechanism of cell signalling and regulation. It has been implicated recently in defence strategies against a variety of pathogens that alter host signalling pathways in order to facilitate their invasion and survival within host cells. In this study, the involvement of protein kinases (PKs) has been investigated in attachment and invasion by the pathogenic fungus Fonsecaea pedrosoi within epithelial cells and macrophages. The use of the PK inhibitors staurosporine, genistein and calphostin C prior to infection provided significant information about the role played by PKs in the F. pedrosoi–host cell interaction. All three PK inhibitors could reduce cell invasion by F. pedrosoi significantly. Pre-treatment of macrophages, epithelial cells or conidia with PK inhibitors decreased fungus invasion, and this effect could be overcome by okadaic acid, a phosphatase inhibitor. Immunofluorescence assays showed that tyrosine residues were phosphorylated in the first step of the interaction, while serine residues were phosphorylated in the subsequent step of entry of the parasite into the host cell. These results suggest that both host-cell and conidium PK activities are important in the interaction process, playing a significant role in cell invasion.
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Comparison of Fonsecaea pedrosoi sclerotic cells obtained in vivo and in vitro: ultrastructure and antigenicity
FEMS Immunology and Medical Microbiology, 2002Co-Authors: Jorge Pereira Da Silva, Celuta S. Alviano, Wanderley De Souza, Daniela S. Alviano, José Antonio Picanço Diniz, Luiz R. Travassos, Sonia RozentalAbstract:The parasitic form of Fonsecaea pedrosoi from the hyperkeratotic layer of the skin was obtained from four patients with chromoblastomycosis. Primary cultures containing hyphae and conidia were successfully converted into sclerotic cells in the presence of 800 μM propranolol and low pH as described before [1]. The morphology of sclerotic cells of F. pedrosoi obtained in vivo and in vitro was analyzed by light and electron microscopy. Their antigenicity was also compared by immunofluorescence microscopy and ELISA assays, using serum samples from untreated patients infected with F. pedrosoi. Due to the similarity of the sclerotic cells obtained in vivo and in vitro, the latter can be more adequately in studies of host–parasite interactions in chromoblastomycosis.
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morphometric and densitometric study of the biogenesis of electron dense granules in Fonsecaea pedrosoi
Fems Microbiology Letters, 1999Co-Authors: Anderson J. Franzen, Celuta S. Alviano, Wanderley De Souza, Marcos Farina, Sonia RozentalAbstract:Fonsecaea pedrosoi is a polymorphic pathogenic fungus, etiological agent of chromoblastomycosis, that synthesizes a melanin-like pigment. Although this pigment has been described as a component of the outer layers of the cell wall, electron-dense cytoplasmic bodies have also been visualized. In this work, we have correlated the appearance of intracellular electron-dense granules with the melanization process in F. pedrosoi. For this, conidial forms were grown under conditions where melanin was not synthesized. Afterwards, cells were incubated in Hank's medium supplemented with bovine fetal serum, at 37°C, to stimulate the pigment production. The genesis of cytoplasmic bodies, with different stages of electron density, was demonstrated by transmission electron microscopy. The appearance of fungal acidic compartments, visualized by confocal laser scanning microscopy in cells stained with acridine orange, was time coincident with the formation of electron-dense granules observed by transmission electron microscopy. The quantification of granule numbers as well as morphometric and densitometric studies were performed.
Claudio Guedes Salgado - One of the best experts on this subject based on the ideXlab platform.
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Development of Natural Culture Media for Rapid Induction of Fonsecaea pedrosoi Sclerotic Cells In Vitro
2015Co-Authors: Moises Batista Da Silva, Jorge Pereira Da Silva, Suellen Sirleide Pereira Yamano, Ubirajara Imbiriba Salgado, Jose ́ Antonio Picanço Diniz, Claudio Guedes SalgadoAbstract:Fonsecaea pedrosoi is the main agent of chromoblastomycosis, a skin disease presenting verrucous lesions, in which round, thick-walled sclerotic cells are found. In vitro induction of sclerotic cells is time-consuming (20 to 45 days) and temperature dependent. We present two new natural media that reduce the sclerotic-cell induction time to only 2 days. Chromoblastomycosis is a subcutaneous mycosis with verru-cous-nodular lesions, usually localized on the lower limbs of rural workers (12), appearing after accidental inoculation with thorns bearing dematiaceous fungi, such as Fonsecaea pedrosoi or Cladophialophora carrionii (11, 15). Laboratory diagnosis is performed by direct microscopic examination of skin scrapings after treatment with 10 % KOH. Round, brownish fungal cells (called sclerotic or muriform cells) characterized by multisep-tate division are observed in small aggregates or isolated in the lesion (9). The vast majority of in vitro work done with F. pedrosoi
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Development of Natural Culture Media for Rapid Induction of Fonsecaea pedrosoi Sclerotic Cells In Vitro
Journal of Clinical Microbiology, 2008Co-Authors: Moises Batista Da Silva, Jorge Pereira Da Silva, Suellen Sirleide Pereira Yamano, Ubirajara Imbiriba Salgado, José Antonio Picanço Diniz, Claudio Guedes SalgadoAbstract:Fonsecaea pedrosoi is the main agent of chromoblastomycosis, a skin disease presenting verrucous lesions, in which round, thick-walled sclerotic cells are found. In vitro induction of sclerotic cells is time-consuming (20 to 45 days) and temperature dependent. We present two new natural media that reduce the sclerotic-cell induction time to only 2 days.
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Ultrastructural characterization of melanosomes of the human pathogenic fungus Fonsecaea pedrosoi.
Journal of Structural Biology, 2008Co-Authors: Anderson J. Franzen, Wanderley De Souza, Moises Batista Da Silva, Claudio Guedes Salgado, Marcel M.l. Cunha, Kildare Miranda, Joachim Hentschel, Helmut Plattner, Sonia RozentalAbstract:Melanin is a complex polymer widely distributed in nature and has been described as an important virulence factor in pathogenic fungi. In the majority of fungi, the mechanism of melanin formation remains unclear. In Fonsecaea pedrosoi, the major etiologic agent of chromoblastomycosis, melanin is stored in intracellular vesicles, named melanosomes. This paper details the ultrastructural aspects of melanin formation, its storage and transportation to the cell wall in the human pathogenic fungus F. pedrosoi. In this fungus, melanin synthesis within melanosomes also begins with a fibrillar matrix formation, displaying morphological and structural features similar to melanosomes from amphibian and mammalian cells. Silver precipitation based on Fontana-Masson technique for melanin detection and immunocytochemistry showed that melanosome fuses with fungal cell membrane where the melanin is released and reaches the cell wall. Melanin deposition in the fungal cell wall occurs in concentric layers. Antibodies raised against F. pedrosoi melanin revealed the sites of melanin production and storage in the melanosomes. In addition, a preliminary description of the elemental composition of this organelle by X-ray microanalysis and elemental mapping revealed the presence of calcium, phosphorus and iron concentrated in its matrix, suggesting a new functional role for these organelles as iron storage compartments.
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isolation of Fonsecaea pedrosoi from thorns of mimosa pudica a probable natural source of chromoblastomycosis
Revista Do Instituto De Medicina Tropical De Sao Paulo, 2004Co-Authors: Claudio Guedes Salgado, Moises Batista Da Silva, Jorge Pereira Da Silva, José Antonio Picanço Diniz, Claudio Teixeira, Patricia Fagundes Da Costa, Ubirajara Imbiriba SalgadoAbstract:Este trabalho demonstra o isolamento de Fonsecaea pedrosoi de espinhos da planta Mimosa pudica L., a partir do local de suposta infeccao identificado pela paciente infectada. O diagnostico clinico de cromoblastomicose foi estabelecido pelo achado de corpos fumagoides no exame microscopico direto e pelas culturas de F. pedrosoi do material obtido da lesao da paciente. A mesma especie foi isolada da paciente e da planta. A microscopia eletronica de transmissao da superficie dos espinhos evidenciou a disposicao conidial caracteristica de F. pedrosoi. Estes dados indicam que a planta M. pudica deve ser uma fonte natural de infeccao do fungo F. pedrosoi.
Ubirajara Imbiriba Salgado - One of the best experts on this subject based on the ideXlab platform.
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ISOLATION OF Fonsecaea pedrosoi FROM THORNS OF Mimosa pudica, A PROBABLE NATURAL SOURCE OF CHROMOBLASTOMYCOSIS
2015Co-Authors: Claudio Teixeira, Ubirajara Imbiriba SalgadoAbstract:We report the isolation of Fonsecaea pedrosoi from thorns of the plant Mimosa pudica L. at the place of infection identified by one of our patients. Clinical diagnosis of chromoblastomycosis was established by direct microscopic examination and cultures from the patient’s lesion. The same species was isolated from the patient and from the plant. Scanning electron microscopy of the surface of the thorns showed the characteristic conidial arrangement of F. pedrosoi. These data indicate that M. pudica could be a natural source of infection for the fungus F. pedrosoi
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Development of Natural Culture Media for Rapid Induction of Fonsecaea pedrosoi Sclerotic Cells In Vitro
2015Co-Authors: Moises Batista Da Silva, Jorge Pereira Da Silva, Suellen Sirleide Pereira Yamano, Ubirajara Imbiriba Salgado, Jose ́ Antonio Picanço Diniz, Claudio Guedes SalgadoAbstract:Fonsecaea pedrosoi is the main agent of chromoblastomycosis, a skin disease presenting verrucous lesions, in which round, thick-walled sclerotic cells are found. In vitro induction of sclerotic cells is time-consuming (20 to 45 days) and temperature dependent. We present two new natural media that reduce the sclerotic-cell induction time to only 2 days. Chromoblastomycosis is a subcutaneous mycosis with verru-cous-nodular lesions, usually localized on the lower limbs of rural workers (12), appearing after accidental inoculation with thorns bearing dematiaceous fungi, such as Fonsecaea pedrosoi or Cladophialophora carrionii (11, 15). Laboratory diagnosis is performed by direct microscopic examination of skin scrapings after treatment with 10 % KOH. Round, brownish fungal cells (called sclerotic or muriform cells) characterized by multisep-tate division are observed in small aggregates or isolated in the lesion (9). The vast majority of in vitro work done with F. pedrosoi
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Development of Natural Culture Media for Rapid Induction of Fonsecaea pedrosoi Sclerotic Cells In Vitro
Journal of Clinical Microbiology, 2008Co-Authors: Moises Batista Da Silva, Jorge Pereira Da Silva, Suellen Sirleide Pereira Yamano, Ubirajara Imbiriba Salgado, José Antonio Picanço Diniz, Claudio Guedes SalgadoAbstract:Fonsecaea pedrosoi is the main agent of chromoblastomycosis, a skin disease presenting verrucous lesions, in which round, thick-walled sclerotic cells are found. In vitro induction of sclerotic cells is time-consuming (20 to 45 days) and temperature dependent. We present two new natural media that reduce the sclerotic-cell induction time to only 2 days.
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isolation of Fonsecaea pedrosoi from thorns of mimosa pudica a probable natural source of chromoblastomycosis
Revista Do Instituto De Medicina Tropical De Sao Paulo, 2004Co-Authors: Claudio Guedes Salgado, Moises Batista Da Silva, Jorge Pereira Da Silva, José Antonio Picanço Diniz, Claudio Teixeira, Patricia Fagundes Da Costa, Ubirajara Imbiriba SalgadoAbstract:Este trabalho demonstra o isolamento de Fonsecaea pedrosoi de espinhos da planta Mimosa pudica L., a partir do local de suposta infeccao identificado pela paciente infectada. O diagnostico clinico de cromoblastomicose foi estabelecido pelo achado de corpos fumagoides no exame microscopico direto e pelas culturas de F. pedrosoi do material obtido da lesao da paciente. A mesma especie foi isolada da paciente e da planta. A microscopia eletronica de transmissao da superficie dos espinhos evidenciou a disposicao conidial caracteristica de F. pedrosoi. Estes dados indicam que a planta M. pudica deve ser uma fonte natural de infeccao do fungo F. pedrosoi.