The Experts below are selected from a list of 3042 Experts worldwide ranked by ideXlab platform
Ningyi Jin - One of the best experts on this subject based on the ideXlab platform.
-
immune responses of swine inoculated with a recombinant Fowlpox virus co expressing p12a and 3c of fmdv and swine il 18
Veterinary Immunology and Immunopathology, 2008Co-Authors: Ningyi Jin, Guoshun Shen, Min Zheng, Guangze Zhu, Minglan Jin, Xiaowei Huo, Hui Juan Liu, Gefen Yin, Kuoshi JinAbstract:Abstract Two recombinant Fowlpox viruses (rFPV-P1 and rFPV-IL18-2AP12A) containing foot-and-mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 were evaluated to determine their abilities to induce humoral and cellular responses in the presence or absence of swine IL-18 as genetic adjuvant. The ability to protect swine against homologous virus challenge was examined. All swine were given booster vaccinations at 21 days after the initial inoculation and were challenged 10 days after the booster vaccination. Control groups were inoculated with wild-type Fowlpox virus (wtFPV). All animals vaccinated with rFPV-P12A and rFPV-IL18-P12A developed specific anti-FMDV ELISA antibody and neutralizing antibody and T-lymphocyte proliferation was observed. Cellular immune function was evaluated via examination of IFN-γ production in swine peripheral blood serum. The results demonstrate the potential viability of a Fowlpox virus-based recombinant vaccine in the control and prevention of FMDV infections.
-
immune responses of pigs inoculated with a recombinant Fowlpox virus coexpressing gp5 gp3 of porcine reproductive and respiratory syndrome virus and swine il 18
Vaccine, 2007Co-Authors: Guoshun Shen, Ningyi Jin, Kuoshi Jin, Min Zheng, Tianzhong Zhuang, Guangze Zhu, Hongtao Jin, Minglan Jin, Xiaowei Huo, Xiaoguang QinAbstract:Two recombinant Fowlpox viruses (rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3) containing the ORF5/ORF3 cDNAs of PRRSV (strain Chang Chun) and IL-18 of swine were constructed and evaluated for theirs abilities to induce humoral and cellular responses in piglets. In addition, their abilities to protect piglets against homologous virus challenge were examined. All piglets were given booster vaccinations at 21 days after the initial inoculation, and all piglets were challenged at 60 after the initial inoculation. Control groups were inoculated with wild-type Fowlpox virus (wtFPV). All animals vaccinated with rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3 developed specific anti-PRRSV ELISA antibody and neutralizing antibody, as well as T-lymphocyte proliferation response. To evaluate the cellular immune function, IFN-gamma production in pigs serum and T-lymphocytes (CD4 and CD8 T cells) in peripheral blood were examined. Following challenge with a pathogenic strain of PRRSV (strain Chang Chun), piglets inoculated with recombinant Fowlpox virus (rFPV) showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection. The results demonstrated the potential use of a Fowlpox virus-based recombinant vaccine in the control and prevention of PRRSV infections.
-
Antitumor effects of a recombinant Fowlpox virus expressing Apoptin in vivo and in vitro
International journal of cancer, 2006Co-Authors: Ningyi Jin, Hai Lian, Lili Sun, Hongling ZhengAbstract:Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in tumor cells. To explore the use of the Apoptin gene in cancer gene therapy, we constructed a recombinant Fowlpox virus expressing the Apoptin protein (vFV-Apoptin) and compared the tumor-killing activity of the recombinant virus with that of wild-type Fowlpox virus in the human hepatoma cell line HepG2. We found that although cells were somewhat resistant to the basal cytotoxic effect of wild-type Fowlpox virus, infection with vFV-Apoptin caused a pronounced, additional cytotoxic effect. Furthermore, cell death and disruption of tumor integrity were apparent in the vFV-Apoptin-infected cells. We also tested whether Fowlpox virus-mediated expression of Apoptin in tumor cells could stimulate an antitumor effect by injecting aggressive subcutaneous tumors derived from H22 mouse hepatoma cells in C57BL/6 mice with vFV-Apoptin. We found that Fowlpox virus-mediated intratumoral expression of the Apoptin gene can induce protective and therapeutic antitumor effects and significantly increase survival. Taken together, these data indicate that infection of tumors with Fowlpox virus expressing Apoptin inhibits tumor growth, induces apoptosis and may be an effective cancer treatment.
-
construction and immunogenicity of a recombinant Fowlpox virus containing the capsid and 3c protease coding regions of foot and mouth disease virus
Journal of Virological Methods, 2006Co-Authors: Min Zheng, Ningyi Jin, Hongyong Zhang, Minglan Jin, Gefen Yin, Ruilin Wang, Qi LiuAbstract:Foot-and-mouth disease virus (FMDV) is an important pathogen with worldwide economic consequences. Consequently, an important goal is the development of a vaccine that can provide rapid protection while overcoming the potential risk associated with the production of conventional inactivated vaccines. An important secondary feature of the vaccine would be the ability to distinguish vaccinated from infected animals. A recombinant Fowlpox virus (vUTAL3CP1) containing FMDV capsid polypeptide and 3C coding regions of O/NY00 was constructed and evaluated for its ability to induce humoral and cellular responses in mice and guinea pigs. In addition, the ability to protect guinea pigs against homologous virus challenge was examined. Mice and guinea pigs were given booster vaccinations twice and once, respectively, and guinea pigs were challenged 20 days after the booster vaccination. Control groups included animals inoculated with commercial vaccine, Fowlpox virus or phosphate-buffered saline (PBS). All animals vaccinated with vUTAL3CP1 developed specific anti-FMDV antibody and neutralizing antibody, as well as T lymphocyte proliferation response and CTL cytotoxic activity. Three of four guinea pigs vaccinated with vUTAL3CP1 were completely protected from viral challenge. The results demonstrated the potential of a Fowlpox virus-based recombinant FMD vaccine.
-
Construction and characterization of recombinant Fowlpox virus coexpressing HIV-1(CN) gp120 and IL-2.
Journal of virological methods, 2005Co-Authors: Wenzheng Jiang, Ningyi Jin, Shufang Cui, Lishu Zhang, Hongyong Zhang, Hongwei Wang, Wenyu HanAbstract:The acquired immunodeficiency syndrome (AIDS) has now become one of the most serious infectious disease in the world, the safe and effective AIDS vaccines would be the best tools to control the disease. Fowlpox virus has been studied recently as a potential vector for the delivery of heterologous vaccine antigen. In this study, a recombinant Fowlpox virus coexpressing gp120 of Chinese prevalent HIV-1 strain and IL-2 was constructed and the cellular immune responses against HIV-1 gp120 in BALB/c mice were evaluated. Chinese vaccine strain 282E4 of Fowlpox virus was used as the vector to construct the recombinant Fowlpox virus (rFPV) coexpressing HIV-1 gp120 and IL-2 via homologous recombination, and the recombinant virus was identified by PCR and Western blotting. The specific DNA fragment was amplified by PCR from the genomes of rFPV. Western blotting analysis showed that HIV-1 gp120 and IL-2 was expressed not only in chicken embryo fibroblast (CEF) cells infected by rFPV, but also in mammalian cells infected by rFPV. After the recombinant Fowlpox virus was inoculated into BALB/c mice, specific CTL activities in the immunized mice were detected in the spleen. The results demonstrated that rFPV had good immunogenicity and could induce BALB/c mice to produce specific cellular immunity. IL-2 played a role of immunoadjuvant and enhanced the cellular immune response. The study provides the basis for the preparation of a Chinese vaccine candidate against HIV-1.
Matthew Law - One of the best experts on this subject based on the ideXlab platform.
-
Efficacy of DNA and Fowlpox virus priming/boosting vaccines for simian/human immunodeficiency virus.
Journal of virology, 2004Co-Authors: C. J. Dale, R. De Rose, Ivan Stratov, Socheata Chea, David C. Montefiori, Scott Thomson, Ian A. Ramshaw, Barbara E.h. Coupar, David B. Boyle, Matthew LawAbstract:Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and Fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the Fowlpox virus boost was also studied. A coordinated induction of high levels of broadly reactive CD4 and CD8 T-cell immune responses was induced by sequential DNA and Fowlpox virus vaccination. The immunogenicity of regimens utilizing Fowlpox virus coexpressing gamma interferon, a single DNA priming vaccination, or DNA vaccines alone was inferior. Significant control of a virulent SHIV challenge was observed despite a loss of SHIV-specific proliferating T cells. The outcome of challenge with virulent SHIVmn229 correlated with vaccine immunogenicity except that DNA vaccination alone primed for protection almost as effectively as the DNA/Fowlpox virus regimen despite negligible immunogenicity by standard assays. These studies suggest that priming of immunity with DNA and Fowlpox virus vaccines could delay AIDS in humans.
-
efficacy of dna and Fowlpox virus priming boosting vaccines for simian human immunodeficiency virus
Journal of Virology, 2004Co-Authors: C. J. Dale, R. De Rose, Ivan Stratov, Socheata Chea, David C. Montefiori, Scott Thomson, Ian A. Ramshaw, Barbara E.h. Coupar, David B. Boyle, Matthew LawAbstract:Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and Fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the Fowlpox virus boost was also studied. A coordinated induction of high levels of broadly reactive CD4 and CD8 T-cell immune responses was induced by sequential DNA and Fowlpox virus vaccination. The immunogenicity of regimens utilizing Fowlpox virus coexpressing gamma interferon, a single DNA priming vaccination, or DNA vaccines alone was inferior. Significant control of a virulent SHIV challenge was observed despite a loss of SHIV-specific proliferating T cells. The outcome of challenge with virulent SHIVmn229 correlated with vaccine immunogenicity except that DNA vaccination alone primed for protection almost as effectively as the DNA/Fowlpox virus regimen despite negligible immunogenicity by standard assays. These studies suggest that priming of immunity with DNA and Fowlpox virus vaccines could delay AIDS in humans.
C. J. Dale - One of the best experts on this subject based on the ideXlab platform.
-
Efficacy of DNA and Fowlpox virus priming/boosting vaccines for simian/human immunodeficiency virus.
Journal of virology, 2004Co-Authors: C. J. Dale, R. De Rose, Ivan Stratov, Socheata Chea, David C. Montefiori, Scott Thomson, Ian A. Ramshaw, Barbara E.h. Coupar, David B. Boyle, Matthew LawAbstract:Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and Fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the Fowlpox virus boost was also studied. A coordinated induction of high levels of broadly reactive CD4 and CD8 T-cell immune responses was induced by sequential DNA and Fowlpox virus vaccination. The immunogenicity of regimens utilizing Fowlpox virus coexpressing gamma interferon, a single DNA priming vaccination, or DNA vaccines alone was inferior. Significant control of a virulent SHIV challenge was observed despite a loss of SHIV-specific proliferating T cells. The outcome of challenge with virulent SHIVmn229 correlated with vaccine immunogenicity except that DNA vaccination alone primed for protection almost as effectively as the DNA/Fowlpox virus regimen despite negligible immunogenicity by standard assays. These studies suggest that priming of immunity with DNA and Fowlpox virus vaccines could delay AIDS in humans.
-
efficacy of dna and Fowlpox virus priming boosting vaccines for simian human immunodeficiency virus
Journal of Virology, 2004Co-Authors: C. J. Dale, R. De Rose, Ivan Stratov, Socheata Chea, David C. Montefiori, Scott Thomson, Ian A. Ramshaw, Barbara E.h. Coupar, David B. Boyle, Matthew LawAbstract:Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and Fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the Fowlpox virus boost was also studied. A coordinated induction of high levels of broadly reactive CD4 and CD8 T-cell immune responses was induced by sequential DNA and Fowlpox virus vaccination. The immunogenicity of regimens utilizing Fowlpox virus coexpressing gamma interferon, a single DNA priming vaccination, or DNA vaccines alone was inferior. Significant control of a virulent SHIV challenge was observed despite a loss of SHIV-specific proliferating T cells. The outcome of challenge with virulent SHIVmn229 correlated with vaccine immunogenicity except that DNA vaccination alone primed for protection almost as effectively as the DNA/Fowlpox virus regimen despite negligible immunogenicity by standard assays. These studies suggest that priming of immunity with DNA and Fowlpox virus vaccines could delay AIDS in humans.
Kuoshi Jin - One of the best experts on this subject based on the ideXlab platform.
-
immune responses of swine inoculated with a recombinant Fowlpox virus co expressing p12a and 3c of fmdv and swine il 18
Veterinary Immunology and Immunopathology, 2008Co-Authors: Ningyi Jin, Guoshun Shen, Min Zheng, Guangze Zhu, Minglan Jin, Xiaowei Huo, Hui Juan Liu, Gefen Yin, Kuoshi JinAbstract:Abstract Two recombinant Fowlpox viruses (rFPV-P1 and rFPV-IL18-2AP12A) containing foot-and-mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 were evaluated to determine their abilities to induce humoral and cellular responses in the presence or absence of swine IL-18 as genetic adjuvant. The ability to protect swine against homologous virus challenge was examined. All swine were given booster vaccinations at 21 days after the initial inoculation and were challenged 10 days after the booster vaccination. Control groups were inoculated with wild-type Fowlpox virus (wtFPV). All animals vaccinated with rFPV-P12A and rFPV-IL18-P12A developed specific anti-FMDV ELISA antibody and neutralizing antibody and T-lymphocyte proliferation was observed. Cellular immune function was evaluated via examination of IFN-γ production in swine peripheral blood serum. The results demonstrate the potential viability of a Fowlpox virus-based recombinant vaccine in the control and prevention of FMDV infections.
-
immune responses of pigs inoculated with a recombinant Fowlpox virus coexpressing gp5 gp3 of porcine reproductive and respiratory syndrome virus and swine il 18
Vaccine, 2007Co-Authors: Guoshun Shen, Ningyi Jin, Kuoshi Jin, Min Zheng, Tianzhong Zhuang, Guangze Zhu, Hongtao Jin, Minglan Jin, Xiaowei Huo, Xiaoguang QinAbstract:Two recombinant Fowlpox viruses (rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3) containing the ORF5/ORF3 cDNAs of PRRSV (strain Chang Chun) and IL-18 of swine were constructed and evaluated for theirs abilities to induce humoral and cellular responses in piglets. In addition, their abilities to protect piglets against homologous virus challenge were examined. All piglets were given booster vaccinations at 21 days after the initial inoculation, and all piglets were challenged at 60 after the initial inoculation. Control groups were inoculated with wild-type Fowlpox virus (wtFPV). All animals vaccinated with rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3 developed specific anti-PRRSV ELISA antibody and neutralizing antibody, as well as T-lymphocyte proliferation response. To evaluate the cellular immune function, IFN-gamma production in pigs serum and T-lymphocytes (CD4 and CD8 T cells) in peripheral blood were examined. Following challenge with a pathogenic strain of PRRSV (strain Chang Chun), piglets inoculated with recombinant Fowlpox virus (rFPV) showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection. The results demonstrated the potential use of a Fowlpox virus-based recombinant vaccine in the control and prevention of PRRSV infections.
-
Application of NDV cDNA Probe for Identification of the Recombinant Fowlpox Virus
Chinese journal of veterinary science, 2004Co-Authors: Lei-li Jia, Ningyi Jin, Zhi-ping Xia, Kuoshi JinAbstract:By EcoR Ⅴ and Xba I digestion, the plasmid which contains NDV F gene was cut into a cDNA fragment of 340bp. Then the fragment was labelled by digoxigenin. It was described that the probe could hybridize specifically with the plasmids containing NDV F gene, but no hybridization signal was detected in other specimens such as control plasmid pUTA-2 and FPV 282E_(4). The sensitivity detection indicated that the probe could detect a minimal 40pg homology DNA. The probe could strongly hybridize with the recombinant Fowlpox virus vFV282. The result indicated that the probe could be used in screening and identifying of the recombinant Fowlpox virus vFV282.
David B. Boyle - One of the best experts on this subject based on the ideXlab platform.
-
dose response relationship of dna and recombinant Fowlpox virus prime boost hiv vaccines implications for future trials
Human Vaccines, 2006Co-Authors: Robert De Rose, Ian A. Ramshaw, David B. Boyle, Mark T Sullivan, Jane C Dale, Anthony D Kelleher, Sean Emery, David A Cooper, Stephen J KentAbstract:Estimating effective doses of novel HIV vaccines is challenging. Dose-response analyses of DNA and Fowlpox virus HIV vaccines showed that 1 mg of DNA vaccine and 5 x 10(7)pfu of Fowlpox virus booster was immunogenic in macaques. However, this dose was poorly immunogenic in humans. When adjusted for body surface area, the human dose studied was equivalent to a poorly immunogenic lower dose in monkeys. These data provide a rationale for guiding dosing in future trials of HIV vaccine technologies.
-
Efficacy of DNA and Fowlpox virus priming/boosting vaccines for simian/human immunodeficiency virus.
Journal of virology, 2004Co-Authors: C. J. Dale, R. De Rose, Ivan Stratov, Socheata Chea, David C. Montefiori, Scott Thomson, Ian A. Ramshaw, Barbara E.h. Coupar, David B. Boyle, Matthew LawAbstract:Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and Fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the Fowlpox virus boost was also studied. A coordinated induction of high levels of broadly reactive CD4 and CD8 T-cell immune responses was induced by sequential DNA and Fowlpox virus vaccination. The immunogenicity of regimens utilizing Fowlpox virus coexpressing gamma interferon, a single DNA priming vaccination, or DNA vaccines alone was inferior. Significant control of a virulent SHIV challenge was observed despite a loss of SHIV-specific proliferating T cells. The outcome of challenge with virulent SHIVmn229 correlated with vaccine immunogenicity except that DNA vaccination alone primed for protection almost as effectively as the DNA/Fowlpox virus regimen despite negligible immunogenicity by standard assays. These studies suggest that priming of immunity with DNA and Fowlpox virus vaccines could delay AIDS in humans.
-
efficacy of dna and Fowlpox virus priming boosting vaccines for simian human immunodeficiency virus
Journal of Virology, 2004Co-Authors: C. J. Dale, R. De Rose, Ivan Stratov, Socheata Chea, David C. Montefiori, Scott Thomson, Ian A. Ramshaw, Barbara E.h. Coupar, David B. Boyle, Matthew LawAbstract:Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and Fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the Fowlpox virus boost was also studied. A coordinated induction of high levels of broadly reactive CD4 and CD8 T-cell immune responses was induced by sequential DNA and Fowlpox virus vaccination. The immunogenicity of regimens utilizing Fowlpox virus coexpressing gamma interferon, a single DNA priming vaccination, or DNA vaccines alone was inferior. Significant control of a virulent SHIV challenge was observed despite a loss of SHIV-specific proliferating T cells. The outcome of challenge with virulent SHIVmn229 correlated with vaccine immunogenicity except that DNA vaccination alone primed for protection almost as effectively as the DNA/Fowlpox virus regimen despite negligible immunogenicity by standard assays. These studies suggest that priming of immunity with DNA and Fowlpox virus vaccines could delay AIDS in humans.
-
Vaccinating chickens against avian influenza with Fowlpox recombinants expressing the H7 haemagglutinin.
Australian veterinary journal, 2000Co-Authors: David B. Boyle, Paul Selleck, Hans G. HeineAbstract:Objective To evaluate the vaccine efficacy of a Fowlpox virus recombinant expressing the H7 haemagglutinin of avian influenza virus in poultry. Procedure Specific-pathogen-free poultry were vaccinated with Fowlpox recombinants expressing H7 or H1 haemagglutinins of influenza virus. Chickens were vaccinated at 2 or 7 days of age and challenged with virulent Australian avian influenza virus at 10 and 21 days later, respectively. Morbidity and mortality, body weight change and the development of immune responses to influenza haemagglutinin and nucleo-protein were recorded. Results Vaccination of poultry with Fowlpox H7 avian influenza virus recombinants induced protective immune responses. All chickens vaccinated at 7 days of age and challenged 21 days later were protected from death. Few clinical signs of infection developed. In contrast, unvaccinated or chickens vaccinated with a non-recombinant Fowlpox or a Fowlpox expressing the H1 haemagglutinin of human influenza were highly susceptible to avian influenza. All those chickens died within 72 h of challenge. In younger chickens, vaccinated at 2 days of age and challenged 10 days later the protection was lower with 80% of chickens protected from death. Chickens surviving vaccination and challenge had high antibody responses to haemagglutinin and primary antibody responses to nucleoprotein suggesting that although vaccination protected substantially against disease it failed to completely prevent replication of the challenge avian influenza virus. Conclusion Vaccination of chickens with Fowlpox virus expressing the avian influenza H7 haemagglutinin provided good protection against experimental challenge with virulent avian influenza of H7 type. Although eradication will remain the method of first choice for control of avian influenza, in the circumstances of a continuing and widespread outbreak the availability of vaccines based upon Fowlpox recombinants provides an additional method for disease control.
