The Experts below are selected from a list of 36492 Experts worldwide ranked by ideXlab platform
Preeti Verma - One of the best experts on this subject based on the ideXlab platform.
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pH Dependant Fungal Proteins In The ‘green’ Synthesis Of Gold Nanoparticles
Advanced Materials Letters, 2010Co-Authors: Rashmi Sanghi, Preeti VermaAbstract:An efficient, simple and environment friendly biosynthesis of gold nanoparticles (GNPs), mediated by Fungal Proteins of Coriolus versicolor is reported. By altering the reaction conditions, the intracellular synthesis of GNPs on the Fungal mycelium, could be well tailored to produce extracellular GNPs in the aqueous medium. The reaction rate and the morphology of the particles were found to depend on parameters such as pH, incubation temperature and concentration of gold solution. The gold nanoparticles were characterized by UV-Vis, SEM and AFM techniques, demonstrating high stability of gold nanoparticles in aqueous media, via the protein layer. The size of the gold nanoparticles using AFM studies was found to be in the range 5-30 nm. These nanoparticles were found to be highly stable as even after prolonged storage for over 6 months they do not show aggregation. A plausible mechanism explaining the role of different possible Proteins under different conditions, in the formation of gold nanoparticles has been investigated using FTIR. This study represents an important advancement in the use of Fungal protein for the extracellular synthesis of functional gold nanoparticles by a green and mild technique in one pot. Copyright © 2010 VBRI press.
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biomimetic synthesis and characterisation of protein capped silver nanoparticles
Bioresource Technology, 2009Co-Authors: Rashmi Sanghi, Preeti VermaAbstract:Abstract A controlled and up-scalable route for the biosynthesis of silver nanopartilces (NPs) mediated by Fungal Proteins of Coriolus versicolor has been undertaken for the first time. The fungus when challenged with silver nitrate solution accumulated silver NPs on its surface in 72 h which could be reduced to 1 h by tailoring the reaction conditions. Under alkaline conditions, the reaction was much faster and could easily proceed at room temperature even without stirring. The resulting Ag NPs displayed controllable structural and optical properties depending on the experimental parameters such as pH and reaction temperatures. The average size, morphology, and structure of particles were determined by AFM, TEM, XRD and UV/Visible absorption spectrophotometry. Fourier transform infrared study disclosed that the amino groups were bound to the particles, which was accountable for the stability of NPs. It further confirmed the presence of protein as the stabilizing and capping agent surrounding the silver NPs. Experiments were conducted both with, media in which fungus was initially harvested and that of pristine Fungal mycelium alone. Under normal conditions, in the case of media extracellular synthesis took place whereby other than the Fungal Proteins, glucose was also responsible for the reduction. In the case of Fungal mycelium, the intracellular formation of Ag NPs, could be tailored to give both intracellular and extracellular Ag NPs under alkaline conditions whereby the surface S–H groups of the fungus played a major role.
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biomimetic synthesis and characterisation of protein capped silver nanoparticles
Bioresource Technology, 2009Co-Authors: Rashmi Sanghi, Preeti VermaAbstract:Abstract A controlled and up-scalable route for the biosynthesis of silver nanopartilces (NPs) mediated by Fungal Proteins of Coriolus versicolor has been undertaken for the first time. The fungus when challenged with silver nitrate solution accumulated silver NPs on its surface in 72 h which could be reduced to 1 h by tailoring the reaction conditions. Under alkaline conditions, the reaction was much faster and could easily proceed at room temperature even without stirring. The resulting Ag NPs displayed controllable structural and optical properties depending on the experimental parameters such as pH and reaction temperatures. The average size, morphology, and structure of particles were determined by AFM, TEM, XRD and UV/Visible absorption spectrophotometry. Fourier transform infrared study disclosed that the amino groups were bound to the particles, which was accountable for the stability of NPs. It further confirmed the presence of protein as the stabilizing and capping agent surrounding the silver NPs. Experiments were conducted both with, media in which fungus was initially harvested and that of pristine Fungal mycelium alone. Under normal conditions, in the case of media extracellular synthesis took place whereby other than the Fungal Proteins, glucose was also responsible for the reduction. In the case of Fungal mycelium, the intracellular formation of Ag NPs, could be tailored to give both intracellular and extracellular Ag NPs under alkaline conditions whereby the surface S–H groups of the fungus played a major role.
Ping Zhang - One of the best experts on this subject based on the ideXlab platform.
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identification of ophiocordyceps sinensis and its artificially cultured ophiocordyceps mycelia by ultra performance liquid chromatography orbitrap fusion mass spectrometry and chemometrics
Molecules, 2018Co-Authors: Ping Zhang, Feng Wei, Xianlong Cheng, Guifeng Zhang, Bin LiuAbstract:Since the cost of Ophiocordyceps sinensis, an important Fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of Fungal Proteins in wild O. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m/z 713.39) was identified in the wild O. sinensis fruiting body, AVLSDAITLVR (m/z 579.34) was detected in the fermented O. sinensis mycelium powder, FAELLEK (m/z 849.47) was found in the fermented Ophiocordyceps mycelium powder, LESVVTSFTK (m/z 555.80) was discovered in the artificial Ophiocordyceps mycelium powder, and VPSSAVLR (m/z 414.75) was observed in O. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides of O.sinensis and its related products.
Peter L Bergquist - One of the best experts on this subject based on the ideXlab platform.
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heterologous protein expression in filamentous fungi
Trends in Biotechnology, 2005Co-Authors: K Helena M Nevalainen, Peter L Bergquist, Valentino Junior S TeoAbstract:Filamentous fungi are commonly used in the fermentation industry for the large-scale production of Proteins – mainly industrial enzymes. Recent advances in Fungal genomics and related experimental technologies such as gene arrays and proteomics are rapidly changing the approaches to the development and use of filamentous fungi as hosts for the production of both homologous and heterologous gene products. The emphasis is moving towards sourcing new genes of interest through database mining and unravelling the circuits related to Fungal gene regulation, applying, for example, transcriptomics. However, although heterologous Fungal Proteins are efficiently expressed, expression of gene products from other organisms is subject to several bottlenecks that reduce yield. Current approaches emphasize the study of pathways involved in protein modification and degradation in general rather than gene-by-gene approaches.
Bin Liu - One of the best experts on this subject based on the ideXlab platform.
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identification of ophiocordyceps sinensis and its artificially cultured ophiocordyceps mycelia by ultra performance liquid chromatography orbitrap fusion mass spectrometry and chemometrics
Molecules, 2018Co-Authors: Ping Zhang, Feng Wei, Xianlong Cheng, Guifeng Zhang, Bin LiuAbstract:Since the cost of Ophiocordyceps sinensis, an important Fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of Fungal Proteins in wild O. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m/z 713.39) was identified in the wild O. sinensis fruiting body, AVLSDAITLVR (m/z 579.34) was detected in the fermented O. sinensis mycelium powder, FAELLEK (m/z 849.47) was found in the fermented Ophiocordyceps mycelium powder, LESVVTSFTK (m/z 555.80) was discovered in the artificial Ophiocordyceps mycelium powder, and VPSSAVLR (m/z 414.75) was observed in O. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides of O.sinensis and its related products.
Rashmi Sanghi - One of the best experts on this subject based on the ideXlab platform.
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pH Dependant Fungal Proteins In The ‘green’ Synthesis Of Gold Nanoparticles
Advanced Materials Letters, 2010Co-Authors: Rashmi Sanghi, Preeti VermaAbstract:An efficient, simple and environment friendly biosynthesis of gold nanoparticles (GNPs), mediated by Fungal Proteins of Coriolus versicolor is reported. By altering the reaction conditions, the intracellular synthesis of GNPs on the Fungal mycelium, could be well tailored to produce extracellular GNPs in the aqueous medium. The reaction rate and the morphology of the particles were found to depend on parameters such as pH, incubation temperature and concentration of gold solution. The gold nanoparticles were characterized by UV-Vis, SEM and AFM techniques, demonstrating high stability of gold nanoparticles in aqueous media, via the protein layer. The size of the gold nanoparticles using AFM studies was found to be in the range 5-30 nm. These nanoparticles were found to be highly stable as even after prolonged storage for over 6 months they do not show aggregation. A plausible mechanism explaining the role of different possible Proteins under different conditions, in the formation of gold nanoparticles has been investigated using FTIR. This study represents an important advancement in the use of Fungal protein for the extracellular synthesis of functional gold nanoparticles by a green and mild technique in one pot. Copyright © 2010 VBRI press.
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biomimetic synthesis and characterisation of protein capped silver nanoparticles
Bioresource Technology, 2009Co-Authors: Rashmi Sanghi, Preeti VermaAbstract:Abstract A controlled and up-scalable route for the biosynthesis of silver nanopartilces (NPs) mediated by Fungal Proteins of Coriolus versicolor has been undertaken for the first time. The fungus when challenged with silver nitrate solution accumulated silver NPs on its surface in 72 h which could be reduced to 1 h by tailoring the reaction conditions. Under alkaline conditions, the reaction was much faster and could easily proceed at room temperature even without stirring. The resulting Ag NPs displayed controllable structural and optical properties depending on the experimental parameters such as pH and reaction temperatures. The average size, morphology, and structure of particles were determined by AFM, TEM, XRD and UV/Visible absorption spectrophotometry. Fourier transform infrared study disclosed that the amino groups were bound to the particles, which was accountable for the stability of NPs. It further confirmed the presence of protein as the stabilizing and capping agent surrounding the silver NPs. Experiments were conducted both with, media in which fungus was initially harvested and that of pristine Fungal mycelium alone. Under normal conditions, in the case of media extracellular synthesis took place whereby other than the Fungal Proteins, glucose was also responsible for the reduction. In the case of Fungal mycelium, the intracellular formation of Ag NPs, could be tailored to give both intracellular and extracellular Ag NPs under alkaline conditions whereby the surface S–H groups of the fungus played a major role.
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biomimetic synthesis and characterisation of protein capped silver nanoparticles
Bioresource Technology, 2009Co-Authors: Rashmi Sanghi, Preeti VermaAbstract:Abstract A controlled and up-scalable route for the biosynthesis of silver nanopartilces (NPs) mediated by Fungal Proteins of Coriolus versicolor has been undertaken for the first time. The fungus when challenged with silver nitrate solution accumulated silver NPs on its surface in 72 h which could be reduced to 1 h by tailoring the reaction conditions. Under alkaline conditions, the reaction was much faster and could easily proceed at room temperature even without stirring. The resulting Ag NPs displayed controllable structural and optical properties depending on the experimental parameters such as pH and reaction temperatures. The average size, morphology, and structure of particles were determined by AFM, TEM, XRD and UV/Visible absorption spectrophotometry. Fourier transform infrared study disclosed that the amino groups were bound to the particles, which was accountable for the stability of NPs. It further confirmed the presence of protein as the stabilizing and capping agent surrounding the silver NPs. Experiments were conducted both with, media in which fungus was initially harvested and that of pristine Fungal mycelium alone. Under normal conditions, in the case of media extracellular synthesis took place whereby other than the Fungal Proteins, glucose was also responsible for the reduction. In the case of Fungal mycelium, the intracellular formation of Ag NPs, could be tailored to give both intracellular and extracellular Ag NPs under alkaline conditions whereby the surface S–H groups of the fungus played a major role.
