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Keqin Zhang - One of the best experts on this subject based on the ideXlab platform.
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comparative transcriptomics reveals features and possible mechanisms of glucose mediated soil fungistasis relief in arthrobotrys oligospora
Frontiers in Microbiology, 2020Co-Authors: Tong Liu, Mingliang Zhu, Keqin Zhang, Ying Huang, Xiangxiang Chen, Xi Long, Yunhe YangAbstract:Soil-borne pest diseases result in large annual agricultural losses globally. Fungal bio-control agents are an alternative means of controlling pest diseases; however, soil fungistasis limits the effect of fungal agents. Nutrients can relieve soil fungistasis, but the mechanisms behind this process remain poorly understood. In this study, we determined and quantified the transcriptomes of Arthrobotrys oligospora, a nematode-trapping fungus, derived from samples of fresh conidia, germinated conidia, soil Fungistatic conidia, and glucose-relieved conidia. The transcriptomes of Fungistatic and glucose-relieved conidia were significantly different from those of the other two conidia samples. KEGG pathway analyses showed that those genes upregulated in Fungistatic and glucose-relieved conidia were mainly involved in translation and substance metabolism, and the downregulated genes were mainly involved in MAPK pathway, autophagy, mitophagy, and endocytosis. As being different from the transcriptome of Fungistatic conidia, upregulated genes in the transcriptome of glucose-relieved conidia are also related to replication and repair, spliceosome, oxidative phosphorylation, autophagy, and degradation pathway (lysosome, proteasome, and RNA degradation). And the upregulated genes resulted from comparison of glucose-relieved conidia and Fungistatic conidia were enriched in metabolic pathways, cycle, DNA replication, and repair. The differentially splicing events in the transcriptome of glucose-relieved conidia are far more than that of other two transcriptomes, and genes regulated by differentially splicing were analyzed through KEGG pathway analysis. Furthermore, autophagy genes were proved to play important role in resisting soil fungistasis and glucose-mediated soil fungistasis relief. These data indicate that, in addition to being a carbon and energy source for conidia germination, glucose may also help to relieve soil fungistasis by activating many cellular processes, including autophagy, DNA replication and repair, RNA alternative splicing, and degradation pathways.
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proteomic changes in arthrobotrys oligospora conidia in response to benzaldehyde induced Fungistatic stress
Journal of Proteomics, 2019Co-Authors: Tong Liu, Lijuan Zou, Dongwei Tian, Qiyan Can, Mingliang Zhu, Keqin ZhangAbstract:Abstract Soil fungistasis limits the effect of fungal agents designed to control plant-parasitic nematodes. Benzaldehyde is a Fungistatic factor produced by soil microorganisms that can suppress conidial germination, but the molecular mechanism of this suppression is unknown. In this study, three conidial proteomes of Arthrobotrys oligospora ATCC24927, a nematode-trapping fungus, were obtained, quantified, and compared. Under benzaldehyde Fungistatic stress, conidial protein expression profile changed significantly. Screening with a twofold selection criterion revealed 164 up-regulated and 110 down-regulated proteins. 17 proteins related to protein translation were down-regulated and gene transcription analysis suggested that the repression of proteins translation might be one mechanism by which benzaldehyde inhibites conidial germination. Benzaldehyde also resulted in the down-regulation of respiratory chain proteins and mitochondrial processes, as well as the repression of conidial DNA synthesis. In addition, the conidia up-regulated several proteins that enable it to resist benzaldehyde-induced fungistatis, and this was confirmed by a functional assessment of two knockout mutants. This study reveals putative mechanisms by which benzaldehyde causes fungistasis as well as the proteomic response of conidia to benzaldehyde. Significance Soil fungistasis limits the effect of fungal agents designed to control plant-parasitic nematodes. Benzaldehyde is one of Fungistatic factors produced by soil microorganisms that can suppress conidial germination. In this study, we found that conidial protein expression profile changed significantly under benzaldehyde Fungistatic stress. This research revealed new mechanistic data that describe how benzaldehyde is responsible for fungiststis by inhibiting conidial germination. Moreover, we also found that conidia can resist benzaldehyde by up-regulating proteins such as benzaldehyde dehydrogenase and heat shock proteins. This study also showed that proteomics methods play important roles in addressing soil Fungistatic mechanisms.
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quantitative proteomics revealed partial Fungistatic mechanism of ammonia against conidial germination of nematode trapping fungus arthrobotrys oligospora atcc24927
The International Journal of Biochemistry & Cell Biology, 2018Co-Authors: Tong Liu, Lijuan Zou, Dongwei Tian, Qiyan Can, Mingliang Zhu, Keqin Zhang, Fangyu Liu, Jinkui Yang, Xiaowei HuangAbstract:Ammonia is one of the Fungistatic factors in soil that can suppress conidial germination, but the molecular mechanism underlying the suppression is unknown. In this study, the proteomes of Fungistatic conidia, fresh conidia and germinated conidia of Arthrobotrys oligospora ATCC24927 were determined and quantified. The protein expression profile of Fungistatic conidia was significantly different from those in the other two conditions. 281 proteins were down expressed in Fungistatic conidia and characterized by GO annotation. Gene transcription analysis and inhibition of puromycin (a protein translation inhibitor) on conidial germination suggested that down expression of 33 protein translation related proteins might well result in repression of protein synthesis and inhibition of conidial germination. In addition, 16 down-expressed proteins were mapped to the Ras/mitogen-activated protein (Ras/MAP) regulatory networks which regulate conidial DNA synthesis. The conidial DNA synthesis was found to be definitely inhibited under by ammonia, and function studies of two Ras/MAP proteins by using knock-out strains provided partial evidence that Ras/MAP pathway regulate the conidial germination. These results suggested that down-expression of Ras/MAP related proteins might result in inhibition of DNA synthesis and finally result in inhibition conidial germination. This study revealed partial Fungistatic mechanism of ammonia against conidial germination.
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Phylogenetic analysis on the bacteria producing non-volatile Fungistatic substances
Journal of microbiology (Seoul Korea), 2008Co-Authors: Chang-song Zou, Keqin ZhangAbstract:This study characterized the soil bacteria producing non-volatile Fungistatic substances. Among the 2,100 colonies of soil bacteria randomly isolated from seven agricultural soil samples, 518 isolates (24.67% of total) showed Fungistatic activity toward nematophagous fungi Paecilomyces lilacinus and Trichoderma viride by producing non-volatile substances. A phylogenetic analysis based on amplified ribosomal DNA restriction analysis (ARDRA) and 16S rDNA sequence placed the 518 bacteria in three groups of the domain Bacteria: Actinomycetales, Bacillales, and Gammaproteobacteria. Three genera, Arthrobacter, Bacillus, and Pseudomonas, were the most frequently encountered groups.
J.f. Walker - One of the best experts on this subject based on the ideXlab platform.
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Application of Fungistatics in soil reduces N uptake by an arctic ericoid shrub (Vaccinium vitis-idaea)
Lawrence Berkeley National Laboratory, 2010Co-Authors: J.f. WalkerAbstract:Application of Fungistatics in soil reduces N uptake by an arctic ericoid shrub (Vaccinium vitis-idaea) John F. Walker, Division of Biology, Kansas State Univ., Manhattan, Kansas Loretta C. Johnson Nicholas B. Simpson Markus Bill, Lawrence Berkeley National Laboratory, Berkeley, California Ari Jumpponen Abstract: In arctic tundra soil N is highly limiting, N mineralization is slow and organic N greatly exceeds inorganic N. We studied the effects of Fungistatics (azoxystrobin [Quadris®] or propiconazole [Tilt®]) on the fungi isolated from ericaceous plant roots in vitro. In addition to testing the phytotoxicity of the two Fungistatics we also tested their effects on growth and nitrogen uptake of an ericaceous plant (Vaccinium uliginosum) in a closed Petri plate system without root-associated fungi. Finally, to evaluate the Fungistatic effects in an in vivo experiment we applied Fungistatics and nitrogen isotopes to intact tundra soil cores from Toolik Lake, Alaska, and examined the ammonium-N and glycine-N use by Vaccinium vitis-idaea with and without Fungistatics. The experiments on fungal pure cultures showed that Tilt® was more effective in reducing fungal colony growth in vitro than Quadris®, which was highly variable among the fungal strains. Laboratory experiments aiming to test the Fungistatic effects on plant performance in vitro showed that neither Quadris® nor Tilt® affected V. uliginosum growth or N uptake. In this experiment V. uliginosum assimilated more than an order of magnitude more
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Application of Fungistatics in soil reduces N uptake by an arctic ericoid shrub (Vaccinium vitis-idaea) - eScholarship
2010Co-Authors: J.f. WalkerAbstract:Application of Fungistatics in soil reduces N uptake by an arctic ericoid shrub (Vaccinium vitis-idaea) John F. Walker, Division of Biology, Kansas State Univ., Manhattan, Kansas Loretta C. Johnson Nicholas B. Simpson Markus Bill, Lawrence Berkeley National Laboratory, Berkeley, California Ari Jumpponen Abstract: In arctic tundra soil N is highly limiting, N mineralization is slow and organic N greatly exceeds inorganic N. We studied the effects of Fungistatics (azoxystrobin [Quadris®] or propiconazole [Tilt®]) on the fungi isolated from ericaceous plant roots in vitro. In addition to testing the phytotoxicity of the two Fungistatics we also tested their effects on growth and nitrogen uptake of an ericaceous plant (Vaccinium uliginosum) in a closed Petri plate system without root-associated fungi. Finally, to evaluate the Fungistatic effects in an in vivo experiment we applied Fungistatics and nitrogen isotopes to intact tundra soil cores from Toolik Lake, Alaska, and examined the ammonium-N and glycine-N use by Vaccinium vitis-idaea with and without Fungistatics. The experiments on fungal pure cultures showed that Tilt® was more effective in reducing fungal colony growth in vitro than Quadris®, which was highly variable among the fungal strains. Laboratory experiments aiming to test the Fungistatic effects on plant performance in vitro showed that neither Quadris® nor Tilt® affected V. uliginosum growth or N uptake. In this experiment V. uliginosum assimilated more than an order of magnitude more
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Application of Fungistatics in soil reduces N uptake by an arctic ericoid shrub (Vaccinium vitis-idaea).
Mycologia, 2010Co-Authors: J.f. Walker, Loretta C. Johnson, Nicholas B. Simpson, Markus Bill, Ari JumpponenAbstract:In arctic tundra soil N is highly limiting, N mineralization is slow and organic N greatly exceeds inorganic N. We studied the effects of Fungistatics (azoxystrobin [Quadris®] or propiconazole [Tilt®]) on the fungi isolated from ericaceous plant roots in vitro. In addition to testing the phytotoxicity of the two Fungistatics we also tested their effects on growth and nitrogen uptake of an ericaceous plant (Vaccinium uliginosum) in a closed Petri plate system without root-associated fungi. Finally, to evaluate the Fungistatic effects in an in vivo experiment we applied Fungistatics and nitrogen isotopes to intact tundra soil cores from Toolik Lake, Alaska, and examined the ammonium-N and glycine-N use by Vaccinium vitis-idaea with and without Fungistatics. The experiments on fungal pure cultures showed that Tilt® was more effective in reducing fungal colony growth in vitro than Quadris®, which was highly variable among the fungal strains. Laboratory experiments aiming to test the Fungistatic effects on pla...
Ari Jumpponen - One of the best experts on this subject based on the ideXlab platform.
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Application of Fungistatics in soil reduces N uptake by an arctic ericoid shrub (Vaccinium vitis-idaea).
Mycologia, 2010Co-Authors: J.f. Walker, Loretta C. Johnson, Nicholas B. Simpson, Markus Bill, Ari JumpponenAbstract:In arctic tundra soil N is highly limiting, N mineralization is slow and organic N greatly exceeds inorganic N. We studied the effects of Fungistatics (azoxystrobin [Quadris®] or propiconazole [Tilt®]) on the fungi isolated from ericaceous plant roots in vitro. In addition to testing the phytotoxicity of the two Fungistatics we also tested their effects on growth and nitrogen uptake of an ericaceous plant (Vaccinium uliginosum) in a closed Petri plate system without root-associated fungi. Finally, to evaluate the Fungistatic effects in an in vivo experiment we applied Fungistatics and nitrogen isotopes to intact tundra soil cores from Toolik Lake, Alaska, and examined the ammonium-N and glycine-N use by Vaccinium vitis-idaea with and without Fungistatics. The experiments on fungal pure cultures showed that Tilt® was more effective in reducing fungal colony growth in vitro than Quadris®, which was highly variable among the fungal strains. Laboratory experiments aiming to test the Fungistatic effects on pla...
Lynn Epstein - One of the best experts on this subject based on the ideXlab platform.
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Partial characterization of volatile Fungistatic compound(s) from soil
Phytopathology, 1994Co-Authors: J. A. Liebman, Lynn EpsteinAbstract:Many soils contain volatile, water-soluble compound(s) that inhibit germination of Cochliobolus victoriae conidia in the absence of a carbon source. The volatile Fungistatic compound(s) from soil were separated into a cell-free extract. Loss of Fungistatic activity from the extract was time- and temperature-dependent; all activity was lost within 5 min at 90 C, 48 h at 21 C, and 5 days at -70 C. Much of the Fungistatic activity was lost after the soil extract was diluted by 10%, incubated in an uncapped vial, or transferred to a new vial via a gas-tight syringe. Fungistatic activity was not detected in material collected from soil into a liquid N 2 cold trap [...]
Zhang Keqin - One of the best experts on this subject based on the ideXlab platform.
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Soil volatile fungistasis and volatile Fungistatic compounds
Soil Biology and Biochemistry, 2004Co-Authors: Xu Chuankun, Mo Minghe, Zhang Leming, Zhang KeqinAbstract:Abstract Fungistasis is a widespread phenomenon that can be mediated by soil microorganisms and volatile organic compounds (VOCs). The relationship between soil microorganisms and VOCs is still unclear, however, and many Fungistatic compounds remain to be identified. We assessed the effects of soils (soil direct fungistasis) and VOCs produced by natural soils (soil volatile fungistasis) on the spore germination of several fungi. Both strong soil direct fungistasis and soil volatile fungistasis were observed in a wide range of soils. Soil fungistasis and VOC fungistasis were significantly correlated ( P N , N -dimethyloctylamine and nonadecane, were produced by various Fungistatic soils. Moreover, antifungal activity test of above VOCs showed that trimethylamine, benzaldehyde, and N , N -dimethyloctylamine have strong antifungal activity even at low levels (4–12 mg l −1 ).
