The Experts below are selected from a list of 19857 Experts worldwide ranked by ideXlab platform
Richard W Titball - One of the best experts on this subject based on the ideXlab platform.
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Galleria mellonella as an infection model to investigate virulence of vibrio parahaemolyticus
Virulence, 2018Co-Authors: Sariqa Wagley, Richard Borne, Jamie W Harrison, Craig Bakeraustin, Donatella Ottaviani, Francesca Leoni, Varaporn Vuddhakul, Richard W TitballAbstract:Non-toxigenic V. parahaemolyticus isolates (tdh−/trh−/T3SS2−) have recently been isolated from patients with gastroenteritis. In this study we report that the larvae of the wax moth (Galleria mello...
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evaluation of antibiotic efficacy against infections caused by planktonic or biofilm cultures of pseudomonas aeruginosa and klebsiella pneumoniae in Galleria mellonella
International Journal of Antimicrobial Agents, 2015Co-Authors: Richard W Titball, Mark J Sutton, Gabriel Benthall, Rebecca E Touzel, Charlotte K Hind, Rachael J Thomas, Matthew E WandAbstract:The lack of novel antibiotics for more than a decade has placed increased pressure on existing therapies to combat the emergence of multidrug-resistant (MDR) bacterial pathogens. This study evaluated the Galleria mellonella insect model in determining the efficacy of available antibiotics against planktonic and biofilm infections of MDR Pseudomonas aeruginosa and Klebsiella pneumoniae strains in comparison with in vitro minimum inhibitory concentration (MIC) determination. In general, in vitro analysis agreed with the G. mellonella studies, and susceptibility in Galleria identified different drug resistance mechanisms. However, the carbapenems tested appeared to perform better in vivo than in vitro, with meropenem and imipenem able to clear K. pneumoniae and P. aeruginosa infections with strains that had bla(NDM-1) and bla(VIM) carbapenemases. This study also established an implant model in G. mellonella to allow testing of antibiotic efficacy against biofilm-derived infections. A reduction in antibiotic efficacy of amikacin against K. pneumoniae and P. aeruginosa biofilms was observed compared with a planktonic infection. Ciprofloxacin was found to be less effective at clearing a P. aeruginosa biofilm infection compared with a planktonic infection, but no statistical difference was seen between K. pneumoniae biofilm and planktonic infections treated with this antibiotic (P>0.05). This study provides important information regarding the suitability of Galleria as a model for antibiotic efficacy testing both against planktonic and biofilm-derived MDR infections.
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Galleria mellonella as an infection model for campylobacter jejuni virulence
Journal of Medical Microbiology, 2011Co-Authors: Nicola J. Senior, Mary C. Bagnall, Olivia L. Champion, Martin J Woodward, Roberto M La Ragione, Francisco J. Salguero, Richard W TitballAbstract:Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent.
Jose Chaudnetto - One of the best experts on this subject based on the ideXlab platform.
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capacidade reprodutiva de femeas de apanteles Galleriae hymenoptera braconidae em lagartas de Galleria mellonella e achroia grisella lepidoptera pyralidae criadas com dietas diferentes
Iheringia Serie Zoologia, 2004Co-Authors: Giuliano G Zacarin, Nivar Gobbi, Jose ChaudnettoAbstract:The reproductive capacity of females of Apanteles Galleriae (Wilkinson, 1932) was evaluated in fifth instar caterpillars of Galleria mellonella (Linnaeus, 1758) and Achroia grisella (Fabricius, 1754) fed on standard diet and diets enriched with protein. The reproductive capacity of parasitoid females on fifth instar caterpillars of G. mellonella and A. grisella with variable weight was also evaluated. The host weight interfered in the sex ratio of the obtained parasitoids. In heavier caterpillars, the investment in female descendants was greater than in males, and in lighter caterpillars the inverse occurred.
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preferencia de apanteles Galleriae wilkinson hymenoptera braconidae por Galleria mellonella l ou achroia grisella fabricius lepidoptera pyralidae
Neotropical Entomology, 2004Co-Authors: Giuliano G Zacarin, Nivar Gobbi, Jose ChaudnettoAbstract:Caterpillars of Galleria mellonella (L.) and Achroia grisella (Fabricius), considered pests of beehives, were used to test host preference of the parasitoid Apanteles Galleriae Wilkinson. On average, A. Galleriae females originated from a lab rearing on both hosts preferred to parasitize caterpillars of A. grisella in 67% of the tests. The same experiment was repeated using A. Galleriae females that had been reared during five generations in each of the host species, with similar results. A. Galleriae females reared in caterpillars of G. mellonella parasitized A. grisella caterpillars in 64% of the tests, whereas the A. Galleriae females reared in A. grisella parasitized the same species in 63.5% of the tests. This preference was not dependent on the number of host caterpillars used in each test. We conclude that A. grisella is a better host than G. mellonella to rear A. Galleriae under laboratory conditions, and that the parasitoid is a promising natural enemy for the biological control of these species of wax moths because it can be easily reared in both species and none of these hosts show resistance to the parasitoid.
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biological control preferencia de apanteles Galleriae wilkinson hymenoptera braconidae por Galleria mellonella l ou achroia grisella fabricius lepidoptera pyralidae
2004Co-Authors: Giuliano G Zacarin, Nivar Gobbi, Jose ChaudnettoAbstract:Caterpillars of Galleria mellonella (L.) and Achroia grisella (Fabricius), considered pests of beehives, were used to test host preference of the parasitoid Apanteles Galleriae Wilkinson. On average, A. Galleriae females originated from a lab rearing on both hosts preferred to parasitize caterpillars of A. grisella in 67% of the tests. The same experiment was repeated using A. Galleriae females that had been reared during five generations in each of the host species, with similar results. A. Galleriae females reared in caterpillars of G. mellonella parasitized A. grisella caterpillars in 64% of the tests, whereas the A. Galleriae females reared in A. grisella parasitized the same species in 63.5% of the tests. This preference was not dependent on the number of host caterpillars used in each test. We conclude that A. grisella is a better host than G. mellonella to rear A. Galleriae under laboratory conditions, and that the parasitoid is a promising natural enemy for the biological control of these species of wax moths because it can be easily reared in both species and none of these hosts show resistance to the parasitoid.
A. Carolina Monmany-garzia - One of the best experts on this subject based on the ideXlab platform.
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First report and molecular determination of Apanteles Galleriae Wilkinson (Hymenoptera, Braconidae), a parasitoid of the lesser wax moth Achroia grisella F. (Lepidoptera, Pyralidae) in Northwest Argentina
Journal of Apicultural Research, 2019Co-Authors: Alberto Galindo-cardona, Eduardo Martin, Jorge D Achar, Giuliana González-brizuela, S Adriana Salvo, A. Carolina Monmany-garziaAbstract:Achroia grisella and Galleria mellonella (Lepidoptera, Pyralidae) are two of the most common pests in apiaries worldwide since in their larval stage they feed on wax combs that bees use for their o...
Giuliano G Zacarin - One of the best experts on this subject based on the ideXlab platform.
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capacidade reprodutiva de femeas de apanteles Galleriae hymenoptera braconidae em lagartas de Galleria mellonella e achroia grisella lepidoptera pyralidae criadas com dietas diferentes
Iheringia Serie Zoologia, 2004Co-Authors: Giuliano G Zacarin, Nivar Gobbi, Jose ChaudnettoAbstract:The reproductive capacity of females of Apanteles Galleriae (Wilkinson, 1932) was evaluated in fifth instar caterpillars of Galleria mellonella (Linnaeus, 1758) and Achroia grisella (Fabricius, 1754) fed on standard diet and diets enriched with protein. The reproductive capacity of parasitoid females on fifth instar caterpillars of G. mellonella and A. grisella with variable weight was also evaluated. The host weight interfered in the sex ratio of the obtained parasitoids. In heavier caterpillars, the investment in female descendants was greater than in males, and in lighter caterpillars the inverse occurred.
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preferencia de apanteles Galleriae wilkinson hymenoptera braconidae por Galleria mellonella l ou achroia grisella fabricius lepidoptera pyralidae
Neotropical Entomology, 2004Co-Authors: Giuliano G Zacarin, Nivar Gobbi, Jose ChaudnettoAbstract:Caterpillars of Galleria mellonella (L.) and Achroia grisella (Fabricius), considered pests of beehives, were used to test host preference of the parasitoid Apanteles Galleriae Wilkinson. On average, A. Galleriae females originated from a lab rearing on both hosts preferred to parasitize caterpillars of A. grisella in 67% of the tests. The same experiment was repeated using A. Galleriae females that had been reared during five generations in each of the host species, with similar results. A. Galleriae females reared in caterpillars of G. mellonella parasitized A. grisella caterpillars in 64% of the tests, whereas the A. Galleriae females reared in A. grisella parasitized the same species in 63.5% of the tests. This preference was not dependent on the number of host caterpillars used in each test. We conclude that A. grisella is a better host than G. mellonella to rear A. Galleriae under laboratory conditions, and that the parasitoid is a promising natural enemy for the biological control of these species of wax moths because it can be easily reared in both species and none of these hosts show resistance to the parasitoid.
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biological control preferencia de apanteles Galleriae wilkinson hymenoptera braconidae por Galleria mellonella l ou achroia grisella fabricius lepidoptera pyralidae
2004Co-Authors: Giuliano G Zacarin, Nivar Gobbi, Jose ChaudnettoAbstract:Caterpillars of Galleria mellonella (L.) and Achroia grisella (Fabricius), considered pests of beehives, were used to test host preference of the parasitoid Apanteles Galleriae Wilkinson. On average, A. Galleriae females originated from a lab rearing on both hosts preferred to parasitize caterpillars of A. grisella in 67% of the tests. The same experiment was repeated using A. Galleriae females that had been reared during five generations in each of the host species, with similar results. A. Galleriae females reared in caterpillars of G. mellonella parasitized A. grisella caterpillars in 64% of the tests, whereas the A. Galleriae females reared in A. grisella parasitized the same species in 63.5% of the tests. This preference was not dependent on the number of host caterpillars used in each test. We conclude that A. grisella is a better host than G. mellonella to rear A. Galleriae under laboratory conditions, and that the parasitoid is a promising natural enemy for the biological control of these species of wax moths because it can be easily reared in both species and none of these hosts show resistance to the parasitoid.
García, Pilar Benito - One of the best experts on this subject based on the ideXlab platform.
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Marie-Louise de Parme, reine d’Espagne habillée à la française (1751-1819)
Publications de l’Institut de recherches historiques du Septentrion, 2018Co-Authors: García, Pilar BenitoAbstract:Marie-Louise de Parme a été peinte pour la première fois dans le portrait de la famille de don Philippe par Giuseppe Baldrighi en 1757 (Parme, Galleria Nazionale). Baldrighi fit pour la seconde fois le portrait de la future reine d’Espagne peu avant le mariage de celle-ci avec le prince des Asturies (Madrid, El Pardo, palais royal). De la même période proviennent ses portraits par Laurent Pécheux (New York, Metropolitan Museum of Art ; Parme, Galleria Nazionale ; Florence, palais Pitti). La jeune princesse y est représentée suivant fidèlement la mode française. Vivant en Espagne, la princesse des Asturies se fournissait en France et dans son royaume pour sa garde-robe. Les achats en France étaient faits auprès de Rose Bertin dans les années 1780, époque de laquelle date son portrait par Marino Salvador Maella (Madrid, monastère royal de l’Incarnation). L’examen des longues factures de Rose Bertin peut être utile pour proposer une datation plus précise de ce portrait. Au début du XIXe siècle, le portrait le plus important pour étudier le goût français de la reine est son portrait par Francisco de Goya (Madrid, Palacio Real). Ces années-là, Marie-Louise passa de fort coûteuses commandes auprès de madame Minette à Paris.María Luisa of Parma was depicted for the first time in the portrait of Don Felipe’s family by Giuseppe Baldrighi in 1757 (Galleria Nazionale, Parma). Shortly before her wedding to the Prince of Asturias in 1765, Baldrighi portrayed the future queen of Spain for the second time (Palacio Real de El Pardo, Madrid). The portraits by Laurent Pécheux (Metropolitan Museum of Art, New York; Galleria Nazionale, Parma; Palazzo Pitti, Florence) are from the same period. In them the young princess is shown faithfully dressed in the French fashion. Living in Spain, the Princess of Asturias purchased her wardrobe from France and from her kingdom. The French commissions were placed with Rose Bertin in the 1780s; the portrait by Mariano Salvador Maella (Real Monasterio de la Encarnación, Madrid) dates from this period. Examination of the lengthy bills from Bertin may help date this portrait more precisely. In the early nineteenth century, the key portrait of the queen where the study of her taste for French fashion is concerned is the one by Francisco de Goya (Palacio Real, Madrid). In this period, María Luisa placed highly expensive orders with Madame Minette in Paris
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Marie-Louise de Parme, reine d’Espagne habillée à la française (1751-1819)
Apparence(s), 2015Co-Authors: García, Pilar BenitoAbstract:Marie-Louise de Parme, reine d’Espagne habillée à la française (1751-1819) - Marie-Louise de Parme a été peinte pour la première fois dans le portrait de la famille de don Philippe par Giuseppe Baldrighi en 1757 (Parme, Galleria Nazionale). Baldrighi fit pour la seconde fois le portrait de la future reine d’Espagne peu avant le mariage de celle-ci avec le prince des Asturies (Madrid, El Pardo, palais royal). De la même période proviennent ses portraits par Laurent Pécheux (New York, Metropolitan Museum of Art ; Parme, Galleria Nazionale ; Florence, palais Pitti). La jeune princesse y est représentée suivant fidèlement la mode française. Vivant en Espagne, la princesse des Asturies se fournissait en France et dans son royaume pour sa garde-robe. Les achats en France étaient faits auprès de Rose Bertin dans les années 1780, époque de laquelle date son portrait par Marino Salvador Maella (Madrid, monastère royal de l’Incarnation). L’examen des longues factures de Rose Bertin peut être utile pour proposer une datation plus précise de ce portrait. Au début du xixe siècle, le portrait le plus important pour étudier le goût français de la reine est son portrait par Francisco de Goya (Madrid, Palacio Real). Ces années-là, Marie-Louise passa de fort coûteuses commandes auprès de madame Minette à Paris.María Luisa of Parma, Queen of Spain, Dressed in the French Fashion (1751-1819) - María Luisa of Parma was depicted for the first time in the portrait of Don Felipe’s family by Giuseppe Baldrighi in 1757 (Galleria Nazionale, Parma). Shortly before her wedding to the Prince of Asturias in 1765, Baldrighi portrayed the future queen of Spain for the second time (Palacio Real de El Pardo, Madrid). The portraits by Laurent Pécheux (Metropolitan Museum of Art, New York ; Galleria Nazionale, Parma ; Palazzo Pitti, Florence) are from the same period. In them the young princess is shown faithfully dressed in the French fashion. Living in Spain, the Princess of Asturias purchased her wardrobe from France and from her kingdom. The French commissions were placed with Rose Bertin in the 1780s ; the portrait by Mariano Salvador Maella (Real Monasterio de la Encarnación, Madrid) dates from this period. Examination of the lengthy bills from Bertin may help date this portrait more precisely. In the early nineteenth century, the key portrait of the queen where the study of her taste for French fashion is concerned is the one by Francisco de Goya (Palacio Real, Madrid). In this period, María Luisa placed highly expensive orders with Madame Minette in Paris
