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Domenico Otranto - One of the best experts on this subject based on the ideXlab platform.

  • molecular comparison of Gasterophilus intestinalis and Gasterophilus nasalis from two distinct areas of poland and italy based on cox1 sequence analysis
    Veterinary Parasitology, 2010
    Co-Authors: Monika Pawlasopiela, łukasz Wojciech, Z Soltysiak, Domenico Otranto, Maciej Ugorski
    Abstract:

    In this study, Gasterophilus intestinalis and Gasterophilus nasalis collected from horses in northeastern Poland and southern Italy were genetically compared. The cox1 sequences of the Polish and Italian G. nasalis larvae revealed a higher degree of geographic genetic diversity, with an intra-specific variation rate of 1.27%, than the G. nasalis specimens collected in Poland (intra-specific variation rate: 0.49%) and those collected in Italy (intra-specific variation rate: 0.58%). However, the level of genetic homology of the Polish and Italian G. intestinalis specimens (intra-specific variation rate: 1.27%) was similar to that of the G. intestinalis larvae collected in northeastern Poland (intra-specific variation rate: 0.94%) and those collected in southern Italy (intra-specific variation rate: 1.16%). Analysis of the restriction enzyme sites in the coxI gene of G. nasalis and G. intestinalis showed that the nucleotide polymorphism (NP) at position 1050 of this gene determines cleavage by MnlI only in G. nasalis, making it possible to differentiate the two species using PCR-RFLP. Interestingly, comparison of the nucleotide sequences of the PCR-amplified coxI gene fragments from the Italian specimens of G. nasalis with other analyzed cox1 genes revealed an additional NP at position 1236 of cox1 gene, recognized by MnlI. The present study shows that G. nasalis specimens from different geographical areas display a level of genetic diversity which can influence PCR-RFLP analysis.

  • species composition of Gasterophilus spp diptera oestridae causing equine gastric myiasis in southern italy parasite biodiversity and risks for extinction
    Veterinary Parasitology, 2005
    Co-Authors: Domenico Otranto, Piermarino Milillo, Gioia Capelli, Douglas D. Colwell
    Abstract:

    Horse gastrointestinal myiasis caused by larvae of Gasterophilus spp. (Diptera, Oestridae) flies has a worldwide distribution and, where present, it is primarily caused by larvae of Gasterophilus intestinalis and Gasterophilus nasalis. Other species, i.e., Gasterophilus inermis, Gasterophilus pecorum and Gasterophilus haemorrhoidalis, present in different or in the same regions of the gastrointestinal tract, were only occasionally reported in very limited areas of eastern European Countries and in central Italy. With the aim to contribute data on the species composition of Gasterophilus and on the seasonal variation of the infection pattern in southern Italy, 152 native horses were necropsied from January to December 2003 and Gasterophilus larvae were collected from the stomach, the small intestine and the rectum of each of them. On the whole, 125 (82.2%) horses were infected by larvae of Gasterophilus spp. and 214 second stage larvae (L2) and 13,342 third stage larvae (L3) collected. Five species of Gasterophilus were identified with the following prevalence: G. intestinalis=95.2%, G. nasalis=44.8%, G. inermis=15.2%, G. pecorum=2.6% and G. haemorrhoidalis=0.8%. L3 were retrieved throughout the observation period with the highest mean burdens from January to August 2003 while the lowest mean was registered from September to November 2003. L2 were collected in February-March 2003 and from September to December 2003. The majority of the animals (n=66, 43.4%) were infected with a single Gasterophilus species, however, 45 animals (29.6%) harboured 2 species, 12 animals (7.9%) 3 species and 2 animals (1.3%) 4 species. The trend of abundance in the L3 of G. intestinalis and G. nasalis, the most represented species, was highly concordant (r=0.5, p<0.001). A retrospective comparison of our results and of other data from four seasons of observation (1983-1986) in central Italy showed that the number of G. inermis, G. pecorum and G. haemorrhoidalis have been decreasing relative to G. intestinalis and G. nasalis which may suggest tendency toward the extinction of the three former species of Gasterophilus.

Douglas D. Colwell - One of the best experts on this subject based on the ideXlab platform.

  • species composition of Gasterophilus spp diptera oestridae causing equine gastric myiasis in southern italy parasite biodiversity and risks for extinction
    Veterinary Parasitology, 2005
    Co-Authors: Domenico Otranto, Piermarino Milillo, Gioia Capelli, Douglas D. Colwell
    Abstract:

    Horse gastrointestinal myiasis caused by larvae of Gasterophilus spp. (Diptera, Oestridae) flies has a worldwide distribution and, where present, it is primarily caused by larvae of Gasterophilus intestinalis and Gasterophilus nasalis. Other species, i.e., Gasterophilus inermis, Gasterophilus pecorum and Gasterophilus haemorrhoidalis, present in different or in the same regions of the gastrointestinal tract, were only occasionally reported in very limited areas of eastern European Countries and in central Italy. With the aim to contribute data on the species composition of Gasterophilus and on the seasonal variation of the infection pattern in southern Italy, 152 native horses were necropsied from January to December 2003 and Gasterophilus larvae were collected from the stomach, the small intestine and the rectum of each of them. On the whole, 125 (82.2%) horses were infected by larvae of Gasterophilus spp. and 214 second stage larvae (L2) and 13,342 third stage larvae (L3) collected. Five species of Gasterophilus were identified with the following prevalence: G. intestinalis=95.2%, G. nasalis=44.8%, G. inermis=15.2%, G. pecorum=2.6% and G. haemorrhoidalis=0.8%. L3 were retrieved throughout the observation period with the highest mean burdens from January to August 2003 while the lowest mean was registered from September to November 2003. L2 were collected in February-March 2003 and from September to December 2003. The majority of the animals (n=66, 43.4%) were infected with a single Gasterophilus species, however, 45 animals (29.6%) harboured 2 species, 12 animals (7.9%) 3 species and 2 animals (1.3%) 4 species. The trend of abundance in the L3 of G. intestinalis and G. nasalis, the most represented species, was highly concordant (r=0.5, p<0.001). A retrospective comparison of our results and of other data from four seasons of observation (1983-1986) in central Italy showed that the number of G. inermis, G. pecorum and G. haemorrhoidalis have been decreasing relative to G. intestinalis and G. nasalis which may suggest tendency toward the extinction of the three former species of Gasterophilus.

F B Scott - One of the best experts on this subject based on the ideXlab platform.

  • scanning electron microscope observations on third instar Gasterophilus nasalis diptera oestridae
    Journal of Medical Entomology, 1999
    Co-Authors: Antonio Cesar Rios Leite, F B Scott, L G Evangelista
    Abstract:

    Scanning electron microscope observations studied the morphology of 3rd-instar Gasterophilus nasalis (L.). Distinctive features are illustrated in a sequence of 16 micrographs, bearing indications of structures considered of special interest. The morphology of G. nasalis is compared with that of other larval bot flies.

  • scanning electron microscoscopy of the second instar larva of Gasterophilus nasalis
    Medical and Veterinary Entomology, 1999
    Co-Authors: Antonio Cesar Rios Leite, F B Scott
    Abstract:

    Summary The second-instar larva of the bot fly Gasterophilus nasalis (L.) (Diptera: Gasterophilidae) is described for the first time, based on scanning electron microscope (SEM) studies. On the pseudocephalum the larva bears an anteno-maxillary sensory complex formed by the antenna (coeloconic sensilla) and the maxillary palp with a set of six coeloconic sensilla and four basiconica sensilla. The oral opening is latero-posteriorly limited by small spines, and exhibits strongly ornamented maxillae and mandibles. The thoracic and abdominal segments are circled by two bands, each with two rows (except the last segment that has one row) of backwardly pointed spines, and have cuticular depressions. Trichoid and campaniform sensilla surround the larval segments. The anterior spiracular opening is a small aperture. The terminal end of the eighth abdominal segment shows a spiracular cavity, lateral tubercles, eight basiconic and two trichoid sensilla. Each spiracular plate has two slightly curved slits, each with a serrated rima. There is a probable ecdysial scar. The findings of this ultrastructural study are compared with those other of larval flies.

Antonio Cesar Rios Leite - One of the best experts on this subject based on the ideXlab platform.

  • scanning electron microscope observations on third instar Gasterophilus nasalis diptera oestridae
    Journal of Medical Entomology, 1999
    Co-Authors: Antonio Cesar Rios Leite, F B Scott, L G Evangelista
    Abstract:

    Scanning electron microscope observations studied the morphology of 3rd-instar Gasterophilus nasalis (L.). Distinctive features are illustrated in a sequence of 16 micrographs, bearing indications of structures considered of special interest. The morphology of G. nasalis is compared with that of other larval bot flies.

  • scanning electron microscoscopy of the second instar larva of Gasterophilus nasalis
    Medical and Veterinary Entomology, 1999
    Co-Authors: Antonio Cesar Rios Leite, F B Scott
    Abstract:

    Summary The second-instar larva of the bot fly Gasterophilus nasalis (L.) (Diptera: Gasterophilidae) is described for the first time, based on scanning electron microscope (SEM) studies. On the pseudocephalum the larva bears an anteno-maxillary sensory complex formed by the antenna (coeloconic sensilla) and the maxillary palp with a set of six coeloconic sensilla and four basiconica sensilla. The oral opening is latero-posteriorly limited by small spines, and exhibits strongly ornamented maxillae and mandibles. The thoracic and abdominal segments are circled by two bands, each with two rows (except the last segment that has one row) of backwardly pointed spines, and have cuticular depressions. Trichoid and campaniform sensilla surround the larval segments. The anterior spiracular opening is a small aperture. The terminal end of the eighth abdominal segment shows a spiracular cavity, lateral tubercles, eight basiconic and two trichoid sensilla. Each spiracular plate has two slightly curved slits, each with a serrated rima. There is a probable ecdysial scar. The findings of this ultrastructural study are compared with those other of larval flies.

T C G Oliveirasequeira - One of the best experts on this subject based on the ideXlab platform.

  • prevalence and macro and microscopic lesions produced by Gasterophilus nasalis diptera oestridae in the botucatu region sp brazil
    Veterinary Parasitology, 2001
    Co-Authors: Julio Lopes Sequeira, R A Tostes, T C G Oliveirasequeira
    Abstract:

    The objective of the present study was to investigate the prevalence of Gasterophilus nasalis larvae in Botucatu, the central west region of Sao Paulo State, Brazil, and to describe the lesions caused by the parasite. The climate of Botucatu is warm and rainy during the months of December through March and cool and dry during the months of May through August. The prevalence of G. nasalis was 16.84%, and the period of peak infestation corresponded to the months of cold and dry weather. The lesions detected at the sites of larval attachment were examined macro- and microscopically. Erosions and ulcerations of the gastric mucosa and proximal duodenum were the major macroscopic lesions detected. Microscopically, the lesion spectrum ranged from mild inflammatory reactions to extensive necrosis and ulceration.