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L M V Tillekeratne - One of the best experts on this subject based on the ideXlab platform.
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human sperm motility stimulating activity of a sulfono glycolipid isolated from sri lankan marine red alga Gelidiella acerosa
Asian Journal of Andrology, 2001Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V Tillekeratne, A S AmarasekareAbstract:AIM To evaluate the sperm motility stimulating activity of a sulfono glycolipid (S-ACT-1) isolated from Gelidiella acerosa, a Sri Lankan marine red algae. METHODS S-ACT-1, a white amorphous powder was separated from more polar fractions of the hexane soluble of 1:1 CH2Cl2/MeOH extract and subjected to 1H, 13C NMR and IR Spectroscopy after reverse phase HPLC for identification. Effects of S-ACT-1 on human sperm motility was assessed in vitro at 10, 100 and 1,000 microg/mL concentrations at 37 degrees C for 0, 5, 15, 30 and 60 min. RESULTS S-ACT-1 was identified as a glycolipid sulfate. The lower dose increased the sperm motility slightly, whilst the medium dose significantly increased the motility (P < 0.05) from 5 min of incubation reaching a peak at 15 min and the stimulant effect was sustained throughout the experimental period. Furthermore, the medium dose rendered 80% of the immotile viable sperm motile. In contrast, the highest dose impaired the sperm motility. The sperm stimulating activity of S-ACT-1 was dose-dependent and had a bell-shaped dose response curve for all the 5 incubation periods. CONCLUSION S-ACT-1 of Gelidiella acerosa is a Sulfono glycolipid. S-ACT-1 has a potent sperm motility stimulating activity in vitro and has the potential to be developed into a sperm stimulant.
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isolation of a non steroidal contragestative agent from sri lankan marine red alge Gelidiella acerosa
Contraception, 1996Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V TillekeratneAbstract:This study reports the isolation of a non-steroidal contragestative agent from the Sri Lankan marine red alga, Gelidiella acerosa. One-hundred grams of MeOH-CH2Cl2 (1:1) crude extract of G. acerosa was initially partitioned into hexane, CH2Cl2 and EtOAc, respectively, using the modified Kupchan scheme. At each separation step, all the resulting fractions were tested for contragestative effects in pregnant rats. The fertility was assessed in terms of the number of viable implants following oral administration of fractions (co-precipitated with polyvinylpyrrolidone), on day 1 through day 7 of pregnancy. The hexane fraction with highest activity was subjected to gel filtration through Sephadex LH-20, vacuum liquid chromatography over TLC-grade silica, two open-column chromatography runs over silica gel (60-120 mesh), and HPLC to isolate the active component. Nuclear magnetic resonance and infrared spectroscopic analysis revealed that the contragestative agent of G. acerosa is a sphingosine derivative.
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studies on the post coital contraceptive mechanisms of crude extract of sri lankan marine red algae Gelidiella acerosa
Contraception, 1995Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V TillekeratneAbstract:This study investigates the potential post-coital contraceptive mechanisms of the crude extract of Sri Lankan marine red algae, Gelidiella acerosa, using rats. The dose used was 1000 mg/kg/day and the route of administration was oral. The results showed that the crude extract possessed potent post-coital contraceptive activity when administered on days 7-8 of pregnancy indicating the presence of a narrow window in its action. The post-coital contraceptive activity was due to an elevated post-implantation loss (by 89%) resulting from fetal death between days 9-14 of pregnancy. The extract was neither estrogenic nor anti-estrogenic nor stressor but appeared to be anti-progestational (reduced ovarian progesterone output). It is suggested that the crude extract may reduce the ovarian progesterone release possibly via anti-platelet and PGE2-depressing activity.
M Ganesan - One of the best experts on this subject based on the ideXlab platform.
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impact of cultivation on growth rate and agar content of Gelidiella acerosa gelidiales rhodophyta
Algal Research-Biomass Biofuels and Bioproducts, 2015Co-Authors: M Ganesan, C R K Reddy, Bhavanath JhaAbstract:Abstract Tissue cultured progenies of callus regenerated Gelidiella acerosa were successfully transferred from the laboratory, then were cultivated in the exposed sea. Four plantlets (approx. 0.04 ± 0.01 g fresh wt. and 2–3 cm length) cultured initially in closed net bags in the lagoon waters were multiplied to produce 73 plantlets each with an average biomass of 70 ± 20 g fresh wt.plant − 1 . These plantlets were then shifted to exposed sea and cultivated using the suspended stone method along with wild harvested plantlets. The biomass, Daily Growth Rate, agar yield and quality of the wild and tissue cultured farm plants grown under identical conditions were compared. Biomass of the tissue cultured farm plants ranged from 1.86 ± 1.0 to 2.97 ± 0.7 kg fresh wt.m − 2 , while wild farm plants ranged from 0.44 ± 0.12 to 0.62 ± 0.31 kg fresh wt.m − 2 . Similarly, DGR of the tissue cultured farm plants ranged from 1.81 ± 0.70% to 2.00 ± 0.50% whereas wild farm plants ranged from 1.0 ± 0.40% to 1.2 ± 0.30%. The gel strength of agar extracted from tissue cultured farm plants was higher (2400 ± 250 g cm − 2 than wild farm plants (1900 ± 135 g cm − 2 ). Agar extracted from tissue cultured G. acerosa was found to be suitable for microbial culture and DNA electrophoretic applications. The increased growth and gel strength of the agar recorded in tissue cultured progenies suggested their possible use as improved seed stock for the large scale cultivation of G. acerosa .
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epiphytism differences in Gelidiella acerosa cultivated with floating rafts and concrete blocks
Journal of Applied Phycology, 2015Co-Authors: M Ganesan, K Selvaraj, K Chithra, S SirajudeenAbstract:Seasonal variations in epiphytic assemblages on Gelidiella acerosa cultivated in 2different methods (Floating raft and Bottom concrete block) were studied for 13 months from November 2009 to November 2010 covering all 4 seasons (northeast monsoon, southwest monsoon, autumn and summer). Twenty-five macro algal species were found in raft culture. Chaetomorpha linum Jania adhaerens, Hypnea valentiae, and Lyngbya majuscula were the dominant epiphytes. Twenty-four epiphytic algae were recorded in bottom concrete block culture. Caulerpa racemosa, Caulerpa peltata, Struvea anastomosans, Zonaria variegata, Padina tetrastromatica, Dictyota sp., J. adhaerens, and L. majuscula were the dominant epiphytic species. Seasonal trend in total epiphytic density was the same in both the culture with maximum density during autumn season (192 ± 41 epiphytes m−2 in raft; 498 ± 10 epiphytes m-2 in concrete block) and minimum during northeast monsoon season (112 ± 5 epiphytes m−2 in raft; 247 ± 1 epiphytes m-2 in concrete block). Seasonal variations in dominant epiphyte density followed the trend of total epiphytes with highest in monsoon and autumn seasons. Salinity showed significant positive correlation with epiphyte density (r = 0.63, P = <0.05), while temperature showed strong negative correlation ( r = −0.63, P < 0.05; r = −0.77, P < 0.01). Light microscopic study and scanning electron microscopic study revealed the extent of penetration of epiphytes into host cortex cell. However, this should be confirmed with a transmission electron microscopic study. The results in this paper will be useful to the farmers in understanding the impact of epiphytes on crop yield and define the strategies to avoid epiphytes on G. acerosa in cultivation.
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development of an improved method of cultivation to obtain high biomass of the red alga Gelidiella acerosa gelidiales rhodophyta in the open sea
Biomass & Bioenergy, 2011Co-Authors: M Ganesan, S Thiruppathi, Karuppanan Eswaran, C R K Reddy, Bhavanath JhaAbstract:Abstract This study describes a suspended stone (SS) method developed for in situ propagation of Gelidiella acerosa. The biomass yield potentials with various growth parameters were studied and compared to the floating raft method that was regarded as a superior method for Gelidiella propagation. The biomass of G. acerosa produced by the SS method consistently increased from the first to the fourth harvest (r = 0.940; P 0.05). The biomass of an individual plant harvested from the SS method was significantly higher (P
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cultivation of Gelidiella acerosa in the open sea on the southeastern coast of india
Marine Ecology Progress Series, 2009Co-Authors: M Ganesan, S Thiruppathi, Karuppanan Eswaran, C R K Reddy, B JhaAbstract:Four cultivation methods: raft, bottom net, coral block and cement block were evaluated for the propagation of Gelidiella acerosa in the open sea. Of the 4 methods, the raft method resulted in significantly (p < 0.001) highest daily growth rate (DGR) of 1.11 to 1.31% and biomass of 973 to 1288 g fresh weight (wt) m -2 . Biomass values showed direct correlation with initial seedling densities. Initial seedling densities ≥500 g fresh wt m -2 registered maximum yields of 1425 ± 90 g fresh wt m -2 , while lower initial seedling density (200 g fresh wt m -2 ) showed higher DGR values (0.71 ± 0.07 and 0.48 ± 0.06% during the 1st and 2nd harvests respectively). The 180 d cultivation period registered the highest yield of 367 ± 45 g fresh wt m -2 , which significantly differed (p < 0.001) from values obtained for the 30, 60 and 90 d cultivation periods. The germplasm collected from Sethukarai (sub- tidal reef) showed the highest yield (525 ± 34.6 g fresh wt m -2 , p < 0.001) and DGR (0.54 ± 0.04%) compared with those collected from Pudumadam (intertidal rock), Ervadi (intertidal reef) and Rameswaram (subtidal rock). The findings of this study indicate that the raft method of cultivation using germplasm from Sethukarai, an initial seedling density of ≥500 g fresh wt m -2 and a 180 d cul- tivation period are important criteria for the successful cultivation of G. acerosa. The augmentation of exploited seaweeds through cultivation practices will help preserve natural biodiversity and eco- system functioning.
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agars of Gelidiella acerosa of west and southeast coasts of india
Bioresource Technology, 2007Co-Authors: Kamalesh Prasad, M Ganesan, Bhavanath Jha, B K Ramavat, A K Siddhanta, Pushpito Kumar GhoshAbstract:Abstract Seventeen agar samples were extracted from Gelidiella acerosa (Forsskal) Feldmann and Hamel (Rhodophyta, Gelidiales) specimens collected from nine different sites on the Indian coast—five from southeast coast and four from the west coast. The agar samples were analysed. The stability characteristics of the gels of selected agar samples were studied by rheometry under applied stress conditions, i.e. variation of the storage (G′) and loss moduli (G″) were studied under varying frequency and duration (time) of the stress applied. Yield, apparent and dynamic viscosities, gelling and melting temperatures, 3,6-anhydrogalactose (3,6-AG), sulphate contents and TGA (Thermogravimetric Analysis) measurements of the products were done. It was observed that the best quality agar was produced by G. acerosa occurring in the Gulf of Mannar region in the southeast coast. The gel strengths and the viscosities of agars extracted from Gelidiella acerosa occurring in the Gulf of Mannar ranged from 500 to 700 g cm −2 and 33 to 45 cP for 2001 collections and for 2002 collections the corresponding values were 450 to 845 g cm −2 and 55 to 67 cP respectively. On the other hand, for the agar samples extracted from the west coast of India, the gel strength and viscosities values ranged from 225 to 400 g cm −2 and from 15 to 30 cP, respectively. The agars obtained from G. acerosa collected from southeast coast have been found to be suitable for bacterial culture and molecular biology. This is the first report of superior quality of agar from the Indian agarophytes.
G A S Premakumara - One of the best experts on this subject based on the ideXlab platform.
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human sperm motility stimulating activity of a sulfono glycolipid isolated from sri lankan marine red alga Gelidiella acerosa
Asian Journal of Andrology, 2001Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V Tillekeratne, A S AmarasekareAbstract:AIM To evaluate the sperm motility stimulating activity of a sulfono glycolipid (S-ACT-1) isolated from Gelidiella acerosa, a Sri Lankan marine red algae. METHODS S-ACT-1, a white amorphous powder was separated from more polar fractions of the hexane soluble of 1:1 CH2Cl2/MeOH extract and subjected to 1H, 13C NMR and IR Spectroscopy after reverse phase HPLC for identification. Effects of S-ACT-1 on human sperm motility was assessed in vitro at 10, 100 and 1,000 microg/mL concentrations at 37 degrees C for 0, 5, 15, 30 and 60 min. RESULTS S-ACT-1 was identified as a glycolipid sulfate. The lower dose increased the sperm motility slightly, whilst the medium dose significantly increased the motility (P < 0.05) from 5 min of incubation reaching a peak at 15 min and the stimulant effect was sustained throughout the experimental period. Furthermore, the medium dose rendered 80% of the immotile viable sperm motile. In contrast, the highest dose impaired the sperm motility. The sperm stimulating activity of S-ACT-1 was dose-dependent and had a bell-shaped dose response curve for all the 5 incubation periods. CONCLUSION S-ACT-1 of Gelidiella acerosa is a Sulfono glycolipid. S-ACT-1 has a potent sperm motility stimulating activity in vitro and has the potential to be developed into a sperm stimulant.
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isolation of a non steroidal contragestative agent from sri lankan marine red alge Gelidiella acerosa
Contraception, 1996Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V TillekeratneAbstract:This study reports the isolation of a non-steroidal contragestative agent from the Sri Lankan marine red alga, Gelidiella acerosa. One-hundred grams of MeOH-CH2Cl2 (1:1) crude extract of G. acerosa was initially partitioned into hexane, CH2Cl2 and EtOAc, respectively, using the modified Kupchan scheme. At each separation step, all the resulting fractions were tested for contragestative effects in pregnant rats. The fertility was assessed in terms of the number of viable implants following oral administration of fractions (co-precipitated with polyvinylpyrrolidone), on day 1 through day 7 of pregnancy. The hexane fraction with highest activity was subjected to gel filtration through Sephadex LH-20, vacuum liquid chromatography over TLC-grade silica, two open-column chromatography runs over silica gel (60-120 mesh), and HPLC to isolate the active component. Nuclear magnetic resonance and infrared spectroscopic analysis revealed that the contragestative agent of G. acerosa is a sphingosine derivative.
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studies on the post coital contraceptive mechanisms of crude extract of sri lankan marine red algae Gelidiella acerosa
Contraception, 1995Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V TillekeratneAbstract:This study investigates the potential post-coital contraceptive mechanisms of the crude extract of Sri Lankan marine red algae, Gelidiella acerosa, using rats. The dose used was 1000 mg/kg/day and the route of administration was oral. The results showed that the crude extract possessed potent post-coital contraceptive activity when administered on days 7-8 of pregnancy indicating the presence of a narrow window in its action. The post-coital contraceptive activity was due to an elevated post-implantation loss (by 89%) resulting from fetal death between days 9-14 of pregnancy. The extract was neither estrogenic nor anti-estrogenic nor stressor but appeared to be anti-progestational (reduced ovarian progesterone output). It is suggested that the crude extract may reduce the ovarian progesterone release possibly via anti-platelet and PGE2-depressing activity.
S Hemalatha - One of the best experts on this subject based on the ideXlab platform.
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phytoconstituents from Gelidiella acerosa induce apoptosis by regulating bax bcl2 expression in a549 cells
Biocatalysis and agricultural biotechnology, 2020Co-Authors: S Fazeela Mahaboob M Begum, S HemalathaAbstract:Abstract Phytoconstituents play an important role in human health. They are used in the management of various diseases including cancer, diabetes, cardiovascular, obesity and Alzheimer's. The current study aims to isolate, characterize the phytoconstituents from marine algae Gelidiella acerosa and to determine its anticancer activity under in vitro conditions. The algae was subjected to sequential extraction with solvents like hexane, dichloro methane, ethyl acetate, ethanol, methanol and water. The extracts were analyzed for their phytochemical composition and antiproliferative activity by MTT assay in A549 cells. Among the extracts analyzed, the ethyl acetate extract inhibited the proliferation of A549 cells was selected. Among the four fractions obtained, fraction 1 was analyzed by GC-MS to identify its constituents, evaluated for its anticancer efficiency in A549 cell line by MTT assay, and investigated for the expression of apoptotic biomarkers: Bax, Bcl2, Bcl-XL, Caspase3 and Caspase 8 by Real Time-PCR. The results of GC-MS analysis revealed the presence of 1,2 benzene dicarboxylic acid, mono 2 ethylhexyl ester, 10- octadecanoic acid methyl ester, 1- heneicosyl formate, eicosene, 3- octadecane, 9-octadecanoic acid and 12- hydroxyl methyl ester. The outcomes of anticancer activity showed that the fraction 1 inhibited the proliferation of cancer cells (IC50 15 μg/ml) by inducing cytotoxicity. The results of Real Time PCR showed the activation of Caspase 3 and variation in Bax: Bcl2 ratio indicating the induction of apoptosis by fraction1. These results showed that fraction1 has significant anticancer activity and can be utilized for the development of novel drugs for cancer.
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Gelidiella acerosa inhibits lung cancer proliferation
BMC Complementary and Alternative Medicine, 2018Co-Authors: S Fazeela Mahaboob M Begum, Kalai Chitra, Benin Joseph, Raji Sundararajan, S HemalathaAbstract:Lung adenocarcinoma is the most common subtype of Non small cell lung cancer in which the PI3K/Akt cascade is frequently deregulated. The ubiquitous expression of the PI3K and the frequent inactivation of PTEN accounts for the prolonged survival, evasion of apoptosis and metastasis in cancer. This has led to the development of PI3K inhibitors in the treatment of cancer. Synthetic PI3K inhibitors undergoing clinical and preclinical studies are toxic in animals. Hence, there is a critical need to identify PI3K inhibitor(s) of natural origin. The current study aims to explore the efficacy of the red algae Gelidiella acerosaon inhibition of cell proliferation, migration and the expression of cell survival genes in lung adenocarcinoma cell line A549. The phytoconstituents of Gelidiella acerosa were extracted sequentially with solvents of different polarity, screened qualitatively and quantitatively for secondary metabolites and characterized by GC-MS. The in-vitro studies were performed to check the efficacy of the extract on cell proliferation (MTT assay), cell invasion (scratch assay and colony formation assay), apoptosis (fluorescent, confocal microscopy and flow cytometry) and expression of apoptosis and cell survival proteins including PI3K, Akt and GSK3β and matrix metalloproteinase MMP2 and MMP9 by Western blot method. The antitumor activity of GAE was analyzed in a tumor model of Zebrafish. The outcomes of the in vitro analysis showed an inhibition of cell proliferation, induction of apoptosis, inhibition of cell migration and colonization by the crude extract. The analysis of protein expression showed the activation of caspases 3 and Pro apoptotic protein Bax accompanied by decreased expression of Bcl-2 and Bcl-XL. On the other hand, subsequent activation of GSK3β and down regulation of PI3K, Akt were observed. The decreased expression of MMP2 correlated with the antimetastatic activity of the extract. The in vivo studies showed an inhibition of tumor growth by GAE in Zebrafish. The phytoconstituents of algal extract contributed to the anticancer properties as evidenced by in vitro and in vivo studies. These phytoconstituents can be considered as a natural source of PI3K/Akt inhibitor for treatment of cancers involving the PI3K cascade.
W D Ratnasooriya - One of the best experts on this subject based on the ideXlab platform.
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human sperm motility stimulating activity of a sulfono glycolipid isolated from sri lankan marine red alga Gelidiella acerosa
Asian Journal of Andrology, 2001Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V Tillekeratne, A S AmarasekareAbstract:AIM To evaluate the sperm motility stimulating activity of a sulfono glycolipid (S-ACT-1) isolated from Gelidiella acerosa, a Sri Lankan marine red algae. METHODS S-ACT-1, a white amorphous powder was separated from more polar fractions of the hexane soluble of 1:1 CH2Cl2/MeOH extract and subjected to 1H, 13C NMR and IR Spectroscopy after reverse phase HPLC for identification. Effects of S-ACT-1 on human sperm motility was assessed in vitro at 10, 100 and 1,000 microg/mL concentrations at 37 degrees C for 0, 5, 15, 30 and 60 min. RESULTS S-ACT-1 was identified as a glycolipid sulfate. The lower dose increased the sperm motility slightly, whilst the medium dose significantly increased the motility (P < 0.05) from 5 min of incubation reaching a peak at 15 min and the stimulant effect was sustained throughout the experimental period. Furthermore, the medium dose rendered 80% of the immotile viable sperm motile. In contrast, the highest dose impaired the sperm motility. The sperm stimulating activity of S-ACT-1 was dose-dependent and had a bell-shaped dose response curve for all the 5 incubation periods. CONCLUSION S-ACT-1 of Gelidiella acerosa is a Sulfono glycolipid. S-ACT-1 has a potent sperm motility stimulating activity in vitro and has the potential to be developed into a sperm stimulant.
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isolation of a non steroidal contragestative agent from sri lankan marine red alge Gelidiella acerosa
Contraception, 1996Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V TillekeratneAbstract:This study reports the isolation of a non-steroidal contragestative agent from the Sri Lankan marine red alga, Gelidiella acerosa. One-hundred grams of MeOH-CH2Cl2 (1:1) crude extract of G. acerosa was initially partitioned into hexane, CH2Cl2 and EtOAc, respectively, using the modified Kupchan scheme. At each separation step, all the resulting fractions were tested for contragestative effects in pregnant rats. The fertility was assessed in terms of the number of viable implants following oral administration of fractions (co-precipitated with polyvinylpyrrolidone), on day 1 through day 7 of pregnancy. The hexane fraction with highest activity was subjected to gel filtration through Sephadex LH-20, vacuum liquid chromatography over TLC-grade silica, two open-column chromatography runs over silica gel (60-120 mesh), and HPLC to isolate the active component. Nuclear magnetic resonance and infrared spectroscopic analysis revealed that the contragestative agent of G. acerosa is a sphingosine derivative.
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studies on the post coital contraceptive mechanisms of crude extract of sri lankan marine red algae Gelidiella acerosa
Contraception, 1995Co-Authors: G A S Premakumara, W D Ratnasooriya, L M V TillekeratneAbstract:This study investigates the potential post-coital contraceptive mechanisms of the crude extract of Sri Lankan marine red algae, Gelidiella acerosa, using rats. The dose used was 1000 mg/kg/day and the route of administration was oral. The results showed that the crude extract possessed potent post-coital contraceptive activity when administered on days 7-8 of pregnancy indicating the presence of a narrow window in its action. The post-coital contraceptive activity was due to an elevated post-implantation loss (by 89%) resulting from fetal death between days 9-14 of pregnancy. The extract was neither estrogenic nor anti-estrogenic nor stressor but appeared to be anti-progestational (reduced ovarian progesterone output). It is suggested that the crude extract may reduce the ovarian progesterone release possibly via anti-platelet and PGE2-depressing activity.