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Juan B. Alvarez - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of novel LMW-i glutenin subunit genes from Triticum urartu Thum. ex Gandil.
Theoretical and Applied Genetics, 2015Co-Authors: Susana Cuesta, Carlos Guzman, Juan B. AlvarezAbstract:Key message A high level of Genetic diversity was found in LMW-i genes from Triticum urartu, resulting in detection of 11 novel alleles. The variability detected could affect gluten quality . Abstract Low-molecular weight glutenin subunits are important in determining the viscoelastic properties of wheat dough. Triticum urartu Thum. ex Gandil., which is related to the A genome of polyploid wheat, has been shown as a good source of variation for these subunits. The present study evaluated the variability of LMW-i genes in this species. High polymorphism was found in the sequences analysed and resulted in the detection of 11 novel alleles, classified into two sets (Group-I and -II) showing unique SNPs and InDels. Both groups were associated with Glu - A3 - 1 genes from common wheat. In general, deduced proteins from Group-II genes possessed a higher proportion of glutamine and proline, which has been previously suggested to be related with good quality. Moreover, there were other changes compared to common wheat. This novel variation could affect dough quality. Additional epitopes for celiac disease were also detected, suggesting that these subunits could be highly reactive. The results showed that T. urartu could be an important source of Genetic variability for LMW-i genes that could enlarge the Genetic Pool of modern wheat.
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Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding
Plant Genetic Resources, 2011Co-Authors: M. V. Gutierrez, L M Martin, Carlos Guzman, Juan B. AlvarezAbstract:Triticum urartu Thum. ex Gandil. is a wild species identified as A-genome donor for polyploid wheats, which could be used as gene source for wheat breeding. The high-molecular weight glutenin subunits are endosperm storage proteins that are associated with bread-making quality. In T. urartu , these proteins are encoded by the Ax and Ay genes at the Glu-A u 1 locus. The Ay gene of 17 Glu-A u 1 allelic variants previously detected in this species has been analysed using PCR amplification and digestion of the PCR products with two endonucleases ( Dde I and Pst I). The combination of two restriction patterns has revealed variations between the active and inactive alleles, and within each type. This variation, especially that detected among the active alleles, could enlarge the high-quality Genetic Pool of modern wheat and be used for bread-making quality improvement in durum and common wheat.
Carlos Guzman - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of novel LMW-i glutenin subunit genes from Triticum urartu Thum. ex Gandil.
Theoretical and Applied Genetics, 2015Co-Authors: Susana Cuesta, Carlos Guzman, Juan B. AlvarezAbstract:Key message A high level of Genetic diversity was found in LMW-i genes from Triticum urartu, resulting in detection of 11 novel alleles. The variability detected could affect gluten quality . Abstract Low-molecular weight glutenin subunits are important in determining the viscoelastic properties of wheat dough. Triticum urartu Thum. ex Gandil., which is related to the A genome of polyploid wheat, has been shown as a good source of variation for these subunits. The present study evaluated the variability of LMW-i genes in this species. High polymorphism was found in the sequences analysed and resulted in the detection of 11 novel alleles, classified into two sets (Group-I and -II) showing unique SNPs and InDels. Both groups were associated with Glu - A3 - 1 genes from common wheat. In general, deduced proteins from Group-II genes possessed a higher proportion of glutamine and proline, which has been previously suggested to be related with good quality. Moreover, there were other changes compared to common wheat. This novel variation could affect dough quality. Additional epitopes for celiac disease were also detected, suggesting that these subunits could be highly reactive. The results showed that T. urartu could be an important source of Genetic variability for LMW-i genes that could enlarge the Genetic Pool of modern wheat.
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Molecular characterisation of the amino- and carboxyl-domains in different Glu-A1x alleles of Triticum urartu Thum. ex Gandil
Theoretical and Applied Genetics, 2013Co-Authors: J. B. Alvarez, M. Victoria Gutiérrez, Carlos Guzman, L M MartinAbstract:The wild diploid wheat (Triticum urartu Thum. ex Gandil.) is a potential gene source for wheat breeding, as this species has been identified as the A-genome donor in polyploid wheats. One important wheat breeding trait is bread-making quality, which is associated in bread wheat (T. aestivum ssp. aestivum L. em. Thell.) with the high-molecular-weight glutenin subunits. In T. urartu, these proteins are encoded by the Glu-A1x and Glu-A1Ay genes at the Glu-A (u) 1 locus. The Glu-A1x genes of 12 Glu-A (u) 1 allelic variants previously detected in this species were analysed using PCR amplification and sequencing. Data showed wide diversity for the Glu-A1x alleles in T. urartu, which also showed clear differences to the bread wheat alleles. This variation could enlarge the high-quality Genetic Pool of modern wheat and be used to diversify the bread-making quality in durum (T. turgidum ssp. durum Desf. em. Husn.) and common wheat.
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Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding
Plant Genetic Resources, 2011Co-Authors: M. V. Gutierrez, L M Martin, Carlos Guzman, Juan B. AlvarezAbstract:Triticum urartu Thum. ex Gandil. is a wild species identified as A-genome donor for polyploid wheats, which could be used as gene source for wheat breeding. The high-molecular weight glutenin subunits are endosperm storage proteins that are associated with bread-making quality. In T. urartu , these proteins are encoded by the Ax and Ay genes at the Glu-A u 1 locus. The Ay gene of 17 Glu-A u 1 allelic variants previously detected in this species has been analysed using PCR amplification and digestion of the PCR products with two endonucleases ( Dde I and Pst I). The combination of two restriction patterns has revealed variations between the active and inactive alleles, and within each type. This variation, especially that detected among the active alleles, could enlarge the high-quality Genetic Pool of modern wheat and be used for bread-making quality improvement in durum and common wheat.
L M Martin - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterisation of the amino- and carboxyl-domains in different Glu-A1x alleles of Triticum urartu Thum. ex Gandil
Theoretical and Applied Genetics, 2013Co-Authors: J. B. Alvarez, M. Victoria Gutiérrez, Carlos Guzman, L M MartinAbstract:The wild diploid wheat (Triticum urartu Thum. ex Gandil.) is a potential gene source for wheat breeding, as this species has been identified as the A-genome donor in polyploid wheats. One important wheat breeding trait is bread-making quality, which is associated in bread wheat (T. aestivum ssp. aestivum L. em. Thell.) with the high-molecular-weight glutenin subunits. In T. urartu, these proteins are encoded by the Glu-A1x and Glu-A1Ay genes at the Glu-A (u) 1 locus. The Glu-A1x genes of 12 Glu-A (u) 1 allelic variants previously detected in this species were analysed using PCR amplification and sequencing. Data showed wide diversity for the Glu-A1x alleles in T. urartu, which also showed clear differences to the bread wheat alleles. This variation could enlarge the high-quality Genetic Pool of modern wheat and be used to diversify the bread-making quality in durum (T. turgidum ssp. durum Desf. em. Husn.) and common wheat.
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Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding
Plant Genetic Resources, 2011Co-Authors: M. V. Gutierrez, L M Martin, Carlos Guzman, Juan B. AlvarezAbstract:Triticum urartu Thum. ex Gandil. is a wild species identified as A-genome donor for polyploid wheats, which could be used as gene source for wheat breeding. The high-molecular weight glutenin subunits are endosperm storage proteins that are associated with bread-making quality. In T. urartu , these proteins are encoded by the Ax and Ay genes at the Glu-A u 1 locus. The Ay gene of 17 Glu-A u 1 allelic variants previously detected in this species has been analysed using PCR amplification and digestion of the PCR products with two endonucleases ( Dde I and Pst I). The combination of two restriction patterns has revealed variations between the active and inactive alleles, and within each type. This variation, especially that detected among the active alleles, could enlarge the high-quality Genetic Pool of modern wheat and be used for bread-making quality improvement in durum and common wheat.
Paweł Chmielarz - One of the best experts on this subject based on the ideXlab platform.
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Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm
Annals of Forest Science, 2014Co-Authors: Jan Suszka, Beata Plitta, Marcin Michalak, Barbara Bujarska-borkowska, Tadeusz Tylkowski, Paweł ChmielarzAbstract:Context Black poplar (Populus nigra L.) is an alluvial forest tree species whose Genetic Pool is decreasing in Europe. Poplar trees produce short-lived seeds that do not store well. Aim The feasibility of seed storage in conventional and cryogenic conditions after their desiccation from water content (WC) of 0.15 to 0.07 g H2O g−1 dry mass (g g−1) was investigated. Methods Seed germinability was evaluated (seeds with a radicle and green cotyledons were counted) after storage of seeds for a period of 3 to 24 months at different temperatures: 20°, 10°, 3°, −3°, −10°, −20° or −196°C. Results Seeds desiccated to a 0.07 g g−1 WC can be stored successfully at −10 °C and −20 °C for at least 2 years. A significant decrease in germination was observed only after 12 months of seed storage (WC 0.15 g g−1) at temperatures above 0 °C. We demonstrated that both fresh (0.15 g g−1 WC) and desiccated (0.07 g g−1 WC) seeds can be preserved at −196 °C for at least 2 years. Conclusions Seed storage temperature and time of storage were statistically significant factors affecting seed storability. The presented data provide a foundation for the successful gene banking of P. nigra seeds.
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Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm
Annals of Forest Science, 2014Co-Authors: Jan Suszka, Beata Plitta, Marcin Michalak, Barbara Bujarska-borkowska, Tadeusz Tylkowski, Paweł ChmielarzAbstract:& Context Black poplar (Populus nigra L.) is an alluvial forest tree species whose Genetic Pool is decreasing in Europe. Poplar trees produce short-lived seeds that do not store well. & Aim The feasibility of seed storage in conventional and cryogenic conditions after their desiccation from water content (WC) of 0.15 to 0.07 g H2 Og �1 dry mass (g g �1 )w as investigated. & Methods Seed germinability was evaluated (seeds with a radicle and green cotyledons were counted) after storage of seeds for a period of 3 to 24 months at different temperatures: 20°, 10°, 3°, �3°, �10°, �20° or �196°C.
Susana Cuesta - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of novel LMW-i glutenin subunit genes from Triticum urartu Thum. ex Gandil.
Theoretical and Applied Genetics, 2015Co-Authors: Susana Cuesta, Carlos Guzman, Juan B. AlvarezAbstract:Key message A high level of Genetic diversity was found in LMW-i genes from Triticum urartu, resulting in detection of 11 novel alleles. The variability detected could affect gluten quality . Abstract Low-molecular weight glutenin subunits are important in determining the viscoelastic properties of wheat dough. Triticum urartu Thum. ex Gandil., which is related to the A genome of polyploid wheat, has been shown as a good source of variation for these subunits. The present study evaluated the variability of LMW-i genes in this species. High polymorphism was found in the sequences analysed and resulted in the detection of 11 novel alleles, classified into two sets (Group-I and -II) showing unique SNPs and InDels. Both groups were associated with Glu - A3 - 1 genes from common wheat. In general, deduced proteins from Group-II genes possessed a higher proportion of glutamine and proline, which has been previously suggested to be related with good quality. Moreover, there were other changes compared to common wheat. This novel variation could affect dough quality. Additional epitopes for celiac disease were also detected, suggesting that these subunits could be highly reactive. The results showed that T. urartu could be an important source of Genetic variability for LMW-i genes that could enlarge the Genetic Pool of modern wheat.