The Experts below are selected from a list of 12210 Experts worldwide ranked by ideXlab platform
Yanming Zhu - One of the best experts on this subject based on the ideXlab platform.
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A Glycine Soja group S2 bZIP transcription factor GsbZIP67 conferred bicarbonate alkaline tolerance in Medicago sativa
BMC plant biology, 2018Co-Authors: Pinghui Zhu, Yanming Zhu, Xiaoli Sun, Bowei Jia, Junkai Yang, Yang Shen, Xiaoxi Cai, Mingzhe SunAbstract:Even though bicarbonate alkaline stress is a serious threat to crop growth and yields, it attracts much fewer researches than high salinity stress. The basic leucine zipper (bZIP) transcription factors have been well demonstrated to function in diverse abiotic stresses; however, their biological role in alkaline tolerance still remains elusive. In this study, we functionally characterized a bZIP gene from Glycine Soja GsbZIP67 in bicarbonate alkaline stress responses. GsbZIP67 was initially identified as a putative bicarbonate responsive gene, on the basis of previous RNA-seq data of 50 mM NaHCO3-treated Glycine Soja roots. GsbZIP67 protein possessed a conserved bZIP domain, and belonged to the group S2 bZIP, which is yet less well-studied. Our studies showed that GsbZIP67 targeted to nucleus in Arabidopsis protoplasts, and displayed transcriptional activation activity in yeast cells. The quantitative real-time PCR analyses unraveled the bicarbonate stress responsive expression and tissue specific expression of GsbZIP67 in wild soybean. Further phenotypic analysis illustrated that GsbZIP67 overexpression in alfalfa promoted plant growth under bicarbonate alkaline stress, as evidenced by longer roots and shoots. Furthermore, GsbZIP67 overexpression also modified the physiological indices of transgenic alfalfa under bicarbonate alkaline stress. In addition, the expression levels of several stress responsive genes were also augmented by GsbZIP67 overexpression. Collectively, in this study, we demonstrated that GsbZIP67 acted as a positive regulator of plant tolerance to bicarbonate alkaline stress. These results provide direct genetic evidence of group S2 bZIPs in bicarbonate alkaline stress, and will facilitate further studies concerning the cis-elements and/or downstream genes targeted by GsbZIP67 in stress responses.
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Genome-Wide Analysis of Glycine Soja Response Regulator GsRR Genes Under Alkali and Salt Stresses.
Frontiers in plant science, 2018Co-Authors: Chao Chen, Ailin Liu, Hao Ren, Huizi Duanmu, Xiangbo Duan, Xiaoli Sun, Beidong Liu, Yanming ZhuAbstract:Soil salt-alkalization is a dramatic challenging factor for plant growth. Wild soybean (Glycine Soja) exhibits a favorable trait of superior tolerance to salt-alkali stress, and recent discoveries show that response regulator family genes are involved in diverse abiotic stresses. Genomic and transcriptomic analyses of all response regulator genes in wild soybean will provide insight into their function in plant stress response. In this study, we identified and characterized a total of 56 Glycine Soja response regulator (GsRR) genes. Phylogenetic analysis suggested that GsRR genes could be classified into five subclasses (A1, A2, B1, B2 and C). We further investigated the chromosome locations, gene duplications and conserved domains of the GsRRs. Furthermore, the clustering analysis of GsRR transcript profiles revealed five different expression patterns under alkali stress. The A1 and A2 subclasses display significantly higher transcriptional levels than the B subclass. In addition, quantitative real-time PCR results verified that the GsRR genes were also significantly influenced by salt stress. Notably, GsRR2a in the A1 subclass showed opposite expression patterns under salt stress comparing with alkali stress. Moreover, overexpression of GsRR2a in Arabidopsis significantly improved the tolerance to alkali stress, but not salt stress. These results suggest the important roles of GsRR genes in response to salt and alkaline stresses, and also provide valuable clues for further functional characterization of GsRR family genes.
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A novel Glycine Soja homeodomain-leucine zipper (HD-Zip) I gene, Gshdz4, positively regulates bicarbonate tolerance and responds to osmotic stress in Arabidopsis.
BMC plant biology, 2016Co-Authors: Lei Cao, Yanming Zhu, Chao Chen, Huizi Duanmu, Xiangbo Duan, Pinghui Zhu, Ranran Chen, Xiaodong DingAbstract:Background Wild soybean (Glycine Soja) is a highly adaptive plant species which can grow well in saline-alkaline soils. In soybean genome, there exist about 140 HD-Zip (Homeodomain-leucine Zipper) genes. HD-Zip transcription factor family is one of the largest plant specific superfamilies and plays important roles in response to abiotic stresses. Although HD-Zip transcription factors have been broadly reported to be involved in plant resistance to abiotic stresses like salt and drought, their roles in response to bicarbonate stress is largely unknown.
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A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.
PloS one, 2015Co-Authors: Xiaoli Sun, Chao Chen, Bowei Jia, Yang Shen, Mingzhe Sun, Zhiwei Qin, Kejun Yang, Zhang Meiping, Cong Mingyang, Yanming ZhuAbstract:It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine Soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine Soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.
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GsVAMP72, a novel Glycine Soja R-SNARE protein, is involved in regulating plant salt tolerance and ABA sensitivity
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Xiaoli Sun, Xi Bai, Hua Cai, Mingzhe Sun, Xiaodong Ding, Shanshan Yang, Xue Qian, Yanming ZhuAbstract:Abiotic stress, especially high salinity, is a major threat to agricultural production. It has been well established that SNARE proteins sustain directed vesicle traffic to underpin plant growth and development, yet little is known about the role of SNARE protein in the capacity to withstand abiotic stress, especially in wild soybeans. Here we identified and characterized a GsCBRLK interacting protein, GsVAMP72, which is a putative vesicle-associated membrane protein in Glycine Soja. GsVAMP72 protein has a longin domain at its N-terminus, belonging to R-SNARE family. Quantitative real-time (RT) PCR and beta-glucuronidase (GUS) activity assays revealed that the expression of GsVAMP72 was highly and rapidly induced by both high salt and ABA treatments. Overexpression of GsVAMP72 in Arabidopsis significantly reduced salt tolerance by modifying the ionic content and down-regulating expression of stress-responsive genes, including RD29A, COR47, KIN1, COR15A and RAB18. On the other hand, GsVAMP72 overexpression increased plant ABA sensitivity and altered the expression levels of ABA-responsive genes. Subcellular localization analysis showed that eGFP–GsVAMP72 fusion protein was observed on the plasma membrane-like and endosome-like structures but eGFP alone was distributing throughout the cytoplasm in Arabidopsis protoplasts and onion epidermal cells. GsVAMP72 promoter-controlled GUS activity was detected in both vegetative and reproductive organs, and was strongly induced by salt and ABA. In summary, we demonstrated that GsVAMP72 is a novel Glycine Soja vesicle-associated membrane protein and is highly involved in regulating plant responses to salt and ABA stresses.
Jeong-dong Lee - One of the best experts on this subject based on the ideXlab platform.
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Chromosomal features revealed by comparison of genetic maps of Glycine max and Glycine Soja
Genomics, 2019Co-Authors: Kwanghee Lee, Jeong-dong Lee, Myungshin Kim, Ju Seok Lee, Dong Nyuk Bae, Namhee Jeong, Kiwoung Yang, Jung Ho Park, Jung-kyung Moon, Soon-chun JeongAbstract:Abstract Recombination is a crucial component of evolution and breeding. New combinations of variation on chromosomes are shaped by recombination. Recombination is also involved in chromosomal rearrangements. However, recombination rates vary tremendously among chromosome segments. Genome-wide genetic maps are one of the best tools to study variation of recombination. Here, we describe high density genetic maps of Glycine max and Glycine Soja constructed from four segregating populations. The maps were used to identify chromosomal rearrangements and find the highly predictable pattern of cross-overs on the broad scale in soybean. Markers on these genetic maps were used to evaluate assembly quality of the current soybean reference genome sequence. We find a strong inversion candidate larger than 3 Mb based on patterns of cross-overs. We also identify quantitative trait loci (QTL) that control number of cross-overs. This study provides fundamental insights relevant to practical strategy for breeding programs and for pan-genome researches.
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Isoflavone profile diversity in Korean wild soybeans ( Glycine Soja Sieb. & Zucc.)
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2018Co-Authors: Chigen Tsukamoto, Jeong-dong Lee, Muhammad Amjad Nawaz, Ayaka Kurosaka, Eunho Son, Seung Hwan Yang, Cemal Kurt, Faheem Shehzad Baloch, Gyuhwa ChungAbstract:Isoflavones prevent the incidence of cancer, reduce cardiovascular problems, and decrease menopausal symptoms. In soybean ( Glycine Soja Sieb. & Zucc.) seeds, the content is greatly affected by growing conditions. The variations in soy-isoflavone content were investigated by growing 346 Korean wild soybean accessions from natural habitats under the same agroclimatic conditions. High-performance liquid chromatography was used to measure the profiles of six soy-isoflavones, i.e. glycosides (daidzin, glycitin, genistin) and malonyl glycosides (malonyl-daidzin, malonyl-glycitin, and malonyl-genistin). The soy-isoflavone content of the wild soybeans ranged from 0.5 to 5.51 mg/g with an average of 2.78 mg/g. The highest mean total soy-isoflavone was genistin (1.393 mg/g), followed by daidzin (0.939 mg/g) and glycitin (0.250 mg/g). Korean wild soybean seeds examined in this study were rich in malonyl-genistin. An increasing trend for total soy-isoflavone content was observed from the north to the southeast in the geographical distribution sites, which signifies the effect of genotype and collection site. The information generated in this report will greatly aid soybean breeding stratagems aimed at improving soy-isoflavone concentration in soybean seeds.
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isoflavone profile diversity in korean wild soybeans Glycine Soja sieb zucc
Turkish Journal of Agriculture and Forestry, 2018Co-Authors: Chigen Tsukamoto, Jeong-dong Lee, Muhammad Amjad Nawaz, Ayaka Kurosaka, Eunho Son, Seung Hwan Yang, Cemal Kurt, Faheem Shehzad Baloch, L E Bao, Gyuhwa ChungAbstract:Isoflavones prevent the incidence of cancer, reduce cardiovascular problems, and decrease menopausal symptoms. In soybean ( Glycine Soja Sieb. & Zucc.) seeds, the content is greatly affected by growing conditions. The variations in soy-isoflavone content were investigated by growing 346 Korean wild soybean accessions from natural habitats under the same agroclimatic conditions. High-performance liquid chromatography was used to measure the profiles of six soy-isoflavones, i.e. glycosides (daidzin, glycitin, genistin) and malonyl glycosides (malonyl-daidzin, malonyl-glycitin, and malonyl-genistin). The soy-isoflavone content of the wild soybeans ranged from 0.5 to 5.51 mg/g with an average of 2.78 mg/g. The highest mean total soy-isoflavone was genistin (1.393 mg/g), followed by daidzin (0.939 mg/g) and glycitin (0.250 mg/g). Korean wild soybean seeds examined in this study were rich in malonyl-genistin. An increasing trend for total soy-isoflavone content was observed from the north to the southeast in the geographical distribution sites, which signifies the effect of genotype and collection site. The information generated in this report will greatly aid soybean breeding stratagems aimed at improving soy-isoflavone concentration in soybean seeds.
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Kunitz trypsin inhibitor polymorphism in the Korean wild soybean (Glycine Soja Sieb. & Zucc.)
Plant Breeding, 2013Co-Authors: Panneerselvam Krishnamurthy, R J Singh, Jong Hyun Park, Chigen Tsukamoto, Jeong-dong Lee, Gyuhwa ChungAbstract:Kunitz trypsin inhibitor (KTi) in 1368 accessions of wild soybean (Glycine Soja Sieb. & Zucc.), collected from three regions of Korea, was examined for allelic diversity and geographical distribution. Five electrophoretically distinguishable KTi forms were detected: three were common (Tia, Tib and Tia/Tib) and two were previously unreported (Tibi7-1 and Tibi5). The Tia allele was predominant (93.49%). Alleles Tib, Tibi7-1 and Tibi5 were detected with the frequencies of 3.47, 0.55 and 0.11%, respectively. The heterozygous form (Tia/Tib) was detected with the frequency of 2.26%. The nucleotide sequence of Tibi7-1 was identical to that of the Tib-derived variant allele Tif, with the exception of three nucleotides: AG at position +244, AC at position +286 and GC at position +601. The latter two were similar to Tia, suggesting that Tibi7-1 is an intermediate allele between Tia and Tib. The gene for Tibi5 showed 100% similarity with the Japanese intermediate allele Tibi5. This study demonstrates that Korean wild soybeans are remarkably rich source of new KTi alleles not reported before.
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Saponin polymorphism in the Korean wild soybean (Glycine Soja Sieb. and Zucc.)
Plant Breeding, 2012Co-Authors: Panneerselvam K, Chigen Tsukamoto, Jeong-dong Lee, Seung Hwan Yang, Nozomi Honda, Akio Kikuchi, Gyuhwa ChungAbstract:Seed saponin composition of 3025 wild soybean (Glycine Soja Sieb. and Zucc.) accessions collected from nine regions of Korea was analysed by thin-layer chromatography to determine its polymorphic variation and geographical distribution and find mutants in saponin components. The saponin composition of seed hypocotyls was primarily divided into seven phenotypes, designated as Aa, Ab, AaBc, AbBc, Aa+a, AaBc+a and AbBc+a. The predominant phenotypes were AaBc (55%), Aa (33%), AaBc+a (7.5%) and Aa+a (3.3%). The frequencies of Ab, AbBc and AbBc+a were very low (0.3-0.5%). Codominant alleles Sg-1 a and Sg-1 b and dominant allele Sg-4 occupied 98.6, 1.1 and 63.3%, respectively. Alleles Sg-3 and Sg-5 were found to be dominant in all the analysed accessions except the mutants. Three accessions were discovered as mutants via LC-PDA/MS/MS. The accession CWS0115 did not produce saponin Aa and Ax, CWS2133 did not produce saponin Aa and Ab and CWS5095 did not produce any group A saponins. These newly determined mutants might be utilized in producing a new soybean variety with good taste as well as in biosynthetic studies.
Gyuhwa Chung - One of the best experts on this subject based on the ideXlab platform.
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Korean Wild Soybeans (Glycine Soja Sieb & Zucc.): Geographic Distribution and Germplasm Conservation
Agronomy, 2020Co-Authors: Muhammad Amjad Nawaz, Xiao Lin, Ting-fung Chan, Junghee Ham, Tai-sun Shin, Sezai Ercisli, Kirill S. Golokhvast, Hon-ming Lam, Gyuhwa ChungAbstract:Domesticated crops suffer from major genetic bottlenecks while wild relatives retain higher genomic diversity. Wild soybean (Glycine Soja Sieb. & Zucc.) is the presumed ancestor of cultivated soybean (Glycine max [L.] Merr.), and is an important genetic resource for soybean improvement. Among the East Asian habitats of wild soybean (China, Japan, Korea, and Northeastern Russia), the Korean peninsula is of great importance based on archaeological records, domestication history, and higher diversity of wild soybeans in the region. The collection and conservation of these wild soybean germplasms should be put on high priority. Chung’s Wild Legume Germplasm Collection maintains more than 10,000 legume accessions with an intensive and prioritized wild soybean germplasm collection (>6000 accessions) guided by the international code of conduct for plant germplasm collection and transfer. The center holds a library of unique wild soybean germplasms collected from East Asian wild habitats including the Korean mainland and nearby islands. The collection has revealed interesting and useful morphological, biochemical, and genetic diversity. This resource could be utilized efficiently in ongoing soybean improvement programs across the globe.
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Isoflavone profile diversity in Korean wild soybeans ( Glycine Soja Sieb. & Zucc.)
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2018Co-Authors: Chigen Tsukamoto, Jeong-dong Lee, Muhammad Amjad Nawaz, Ayaka Kurosaka, Eunho Son, Seung Hwan Yang, Cemal Kurt, Faheem Shehzad Baloch, Gyuhwa ChungAbstract:Isoflavones prevent the incidence of cancer, reduce cardiovascular problems, and decrease menopausal symptoms. In soybean ( Glycine Soja Sieb. & Zucc.) seeds, the content is greatly affected by growing conditions. The variations in soy-isoflavone content were investigated by growing 346 Korean wild soybean accessions from natural habitats under the same agroclimatic conditions. High-performance liquid chromatography was used to measure the profiles of six soy-isoflavones, i.e. glycosides (daidzin, glycitin, genistin) and malonyl glycosides (malonyl-daidzin, malonyl-glycitin, and malonyl-genistin). The soy-isoflavone content of the wild soybeans ranged from 0.5 to 5.51 mg/g with an average of 2.78 mg/g. The highest mean total soy-isoflavone was genistin (1.393 mg/g), followed by daidzin (0.939 mg/g) and glycitin (0.250 mg/g). Korean wild soybean seeds examined in this study were rich in malonyl-genistin. An increasing trend for total soy-isoflavone content was observed from the north to the southeast in the geographical distribution sites, which signifies the effect of genotype and collection site. The information generated in this report will greatly aid soybean breeding stratagems aimed at improving soy-isoflavone concentration in soybean seeds.
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isoflavone profile diversity in korean wild soybeans Glycine Soja sieb zucc
Turkish Journal of Agriculture and Forestry, 2018Co-Authors: Chigen Tsukamoto, Jeong-dong Lee, Muhammad Amjad Nawaz, Ayaka Kurosaka, Eunho Son, Seung Hwan Yang, Cemal Kurt, Faheem Shehzad Baloch, L E Bao, Gyuhwa ChungAbstract:Isoflavones prevent the incidence of cancer, reduce cardiovascular problems, and decrease menopausal symptoms. In soybean ( Glycine Soja Sieb. & Zucc.) seeds, the content is greatly affected by growing conditions. The variations in soy-isoflavone content were investigated by growing 346 Korean wild soybean accessions from natural habitats under the same agroclimatic conditions. High-performance liquid chromatography was used to measure the profiles of six soy-isoflavones, i.e. glycosides (daidzin, glycitin, genistin) and malonyl glycosides (malonyl-daidzin, malonyl-glycitin, and malonyl-genistin). The soy-isoflavone content of the wild soybeans ranged from 0.5 to 5.51 mg/g with an average of 2.78 mg/g. The highest mean total soy-isoflavone was genistin (1.393 mg/g), followed by daidzin (0.939 mg/g) and glycitin (0.250 mg/g). Korean wild soybean seeds examined in this study were rich in malonyl-genistin. An increasing trend for total soy-isoflavone content was observed from the north to the southeast in the geographical distribution sites, which signifies the effect of genotype and collection site. The information generated in this report will greatly aid soybean breeding stratagems aimed at improving soy-isoflavone concentration in soybean seeds.
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kunitz trypsin inhibitor polymorphism in the korean wild soybean Glycine Soja sieb zucc
Plant Breeding, 2013Co-Authors: Panneerselvam Krishnamurthy, R J Singh, Jong Hyun Park, Chigen Tsukamoto, Gyuhwa ChungAbstract:Kunitz trypsin inhibitor (KTi) in 1368 accessions of wild soybean (Glycine Soja Sieb. & Zucc.), collected from three regions of Korea, was examined for allelic diversity and geographical distribution. Five electrophoretically distinguishable KTi forms were detected: three were common (Tia, Tib and Tia/Tib) and two were previously unreported (Tibi7-1 and Tibi5). The Tia allele was predominant (93.49%). Alleles Tib, Tibi7-1 and Tibi5 were detected with the frequencies of 3.47, 0.55 and 0.11%, respectively. The heterozygous form (Tia/Tib) was detected with the frequency of 2.26%. The nucleotide sequence of Tibi7-1 was identical to that of the Tib-derived variant allele Tif, with the exception of three nucleotides: AG at position +244, AC at position +286 and GC at position +601. The latter two were similar to Tia, suggesting that Tibi7-1 is an intermediate allele between Tia and Tib. The gene for Tibi5 showed 100% similarity with the Japanese intermediate allele Tibi5. This study demonstrates that Korean wild soybeans are remarkably rich source of new KTi alleles not reported before.
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Kunitz trypsin inhibitor polymorphism in the Korean wild soybean (Glycine Soja Sieb. & Zucc.)
Plant Breeding, 2013Co-Authors: Panneerselvam Krishnamurthy, R J Singh, Jong Hyun Park, Chigen Tsukamoto, Jeong-dong Lee, Gyuhwa ChungAbstract:Kunitz trypsin inhibitor (KTi) in 1368 accessions of wild soybean (Glycine Soja Sieb. & Zucc.), collected from three regions of Korea, was examined for allelic diversity and geographical distribution. Five electrophoretically distinguishable KTi forms were detected: three were common (Tia, Tib and Tia/Tib) and two were previously unreported (Tibi7-1 and Tibi5). The Tia allele was predominant (93.49%). Alleles Tib, Tibi7-1 and Tibi5 were detected with the frequencies of 3.47, 0.55 and 0.11%, respectively. The heterozygous form (Tia/Tib) was detected with the frequency of 2.26%. The nucleotide sequence of Tibi7-1 was identical to that of the Tib-derived variant allele Tif, with the exception of three nucleotides: AG at position +244, AC at position +286 and GC at position +601. The latter two were similar to Tia, suggesting that Tibi7-1 is an intermediate allele between Tia and Tib. The gene for Tibi5 showed 100% similarity with the Japanese intermediate allele Tibi5. This study demonstrates that Korean wild soybeans are remarkably rich source of new KTi alleles not reported before.
Henry T. Nguyen - One of the best experts on this subject based on the ideXlab platform.
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inheritance of salt tolerance in wild soybean Glycine Soja sieb and zucc accession pi483463
Journal of Heredity, 2009Co-Authors: Jeong-dong Lee, Grover J Shannon, Tri D Vuong, Henry T. NguyenAbstract:Tolerant soybean (Glycine max [L.] Merr.) cultivars aid in reducing salt damage in problem fields. New genes are important to reduce losses from salt injury. Objectives of this study were to determine inheritance of salt tolerance in wild soybean (Glycine Soja Sieb. and Zucc.) PI483463 and to test allelism of tolerance genes from genotypes PI483463 and S-100, a common ancestor of southern in US cultivars. Tolerant (T) PI483463 was crossed to sensitive (S) cultivar Hutcheson to study inheritance. PI483463 (T) was crossed with S-100 (T) to test for allelism. Parents, F(1) plants, F(2) populations, and F(2:3) lines were assayed in a 100 mM salt solution to determine tolerance. F(2) from T x S cross segregated 3(T):1 (S) and the F(2:3) lines responded 1 (T): 2 (segregating):1 (S). F(2) plants from PI483463 (T) x S-100 (T) segregated 15 (T):1 (S) indicating different genes from the 2 sources. Results showed that G. Soja line PI483463 had a single dominant gene for salt tolerance, which was different than the gene in G. max line S-100. The symbol, Ncl2, was designated for this new salt tolerance allele.
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Genetic Diversity of Wild Soybean (Glycine Soja Sieb. and Zucc.) Accessions from South Korea and Other Countries
Crop Science, 2008Co-Authors: Jeong-dong Lee, Young-hyun Hwang, Sean M. Blake, Geung-joo Lee, Henry T. Nguyen, J. Grover ShannonAbstract:Wild soybean (Glycine Soja Sieb. and Zucc.) is an important source of genetic variation for introducing useful traits into cultivated soybeans [Glycine max (L.) Merr.]. Little is known about genetic diversity within South Korean wild soybeans and how they differ genetically from other G. Soja lines originating from other regions. Forty-six simple sequence repeat markers covering the 20 soybean linkage groups were used to estimate genetic diversity among 274 wild soybean accessions from South Korea (210), China (34), Japan (25), and eastern Russia (5) and three cultivated checks. Glycine Soja populations from South Korea, China, and Japan all had high genetic diversity with indexes of 0.849, 0.818, and 0.804, respectively. Cluster analyses grouped the 274 accessions into three genetic groups. Cluster I and II consisted of 85 accessions, with 79 of 85 from Korea, only one from China, and five from Japan. Cluster III contained 192 of the 274 G. Soja accessions. Nearly all of the accessions from China and Japan, all from Russia, and 131 of 210 from South Korea were assigned to Group III. However, there was no difference between populations for genetic diversity for South Korea and China. Although it is a very small country, South Korea is a major center of diversity for wild soybeans and potentially a source of useful genes not found in other parts of the world.
Xiaoli Sun - One of the best experts on this subject based on the ideXlab platform.
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A Glycine Soja group S2 bZIP transcription factor GsbZIP67 conferred bicarbonate alkaline tolerance in Medicago sativa
BMC plant biology, 2018Co-Authors: Pinghui Zhu, Yanming Zhu, Xiaoli Sun, Bowei Jia, Junkai Yang, Yang Shen, Xiaoxi Cai, Mingzhe SunAbstract:Even though bicarbonate alkaline stress is a serious threat to crop growth and yields, it attracts much fewer researches than high salinity stress. The basic leucine zipper (bZIP) transcription factors have been well demonstrated to function in diverse abiotic stresses; however, their biological role in alkaline tolerance still remains elusive. In this study, we functionally characterized a bZIP gene from Glycine Soja GsbZIP67 in bicarbonate alkaline stress responses. GsbZIP67 was initially identified as a putative bicarbonate responsive gene, on the basis of previous RNA-seq data of 50 mM NaHCO3-treated Glycine Soja roots. GsbZIP67 protein possessed a conserved bZIP domain, and belonged to the group S2 bZIP, which is yet less well-studied. Our studies showed that GsbZIP67 targeted to nucleus in Arabidopsis protoplasts, and displayed transcriptional activation activity in yeast cells. The quantitative real-time PCR analyses unraveled the bicarbonate stress responsive expression and tissue specific expression of GsbZIP67 in wild soybean. Further phenotypic analysis illustrated that GsbZIP67 overexpression in alfalfa promoted plant growth under bicarbonate alkaline stress, as evidenced by longer roots and shoots. Furthermore, GsbZIP67 overexpression also modified the physiological indices of transgenic alfalfa under bicarbonate alkaline stress. In addition, the expression levels of several stress responsive genes were also augmented by GsbZIP67 overexpression. Collectively, in this study, we demonstrated that GsbZIP67 acted as a positive regulator of plant tolerance to bicarbonate alkaline stress. These results provide direct genetic evidence of group S2 bZIPs in bicarbonate alkaline stress, and will facilitate further studies concerning the cis-elements and/or downstream genes targeted by GsbZIP67 in stress responses.
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Genome-Wide Analysis of Glycine Soja Response Regulator GsRR Genes Under Alkali and Salt Stresses.
Frontiers in plant science, 2018Co-Authors: Chao Chen, Ailin Liu, Hao Ren, Huizi Duanmu, Xiangbo Duan, Xiaoli Sun, Beidong Liu, Yanming ZhuAbstract:Soil salt-alkalization is a dramatic challenging factor for plant growth. Wild soybean (Glycine Soja) exhibits a favorable trait of superior tolerance to salt-alkali stress, and recent discoveries show that response regulator family genes are involved in diverse abiotic stresses. Genomic and transcriptomic analyses of all response regulator genes in wild soybean will provide insight into their function in plant stress response. In this study, we identified and characterized a total of 56 Glycine Soja response regulator (GsRR) genes. Phylogenetic analysis suggested that GsRR genes could be classified into five subclasses (A1, A2, B1, B2 and C). We further investigated the chromosome locations, gene duplications and conserved domains of the GsRRs. Furthermore, the clustering analysis of GsRR transcript profiles revealed five different expression patterns under alkali stress. The A1 and A2 subclasses display significantly higher transcriptional levels than the B subclass. In addition, quantitative real-time PCR results verified that the GsRR genes were also significantly influenced by salt stress. Notably, GsRR2a in the A1 subclass showed opposite expression patterns under salt stress comparing with alkali stress. Moreover, overexpression of GsRR2a in Arabidopsis significantly improved the tolerance to alkali stress, but not salt stress. These results suggest the important roles of GsRR genes in response to salt and alkaline stresses, and also provide valuable clues for further functional characterization of GsRR family genes.
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GsSNAP33, a novel Glycine Soja SNAP25-type protein gene: Improvement of plant salt and drought tolerances in transgenic Arabidopsis thaliana
Plant physiology and biochemistry : PPB, 2017Co-Authors: Zaib-un Nisa, Chao Chen, Xiangbo Duan, Xiaoli Sun, Ali Inayat Mallano, Sikandar Amanullah, Abida Kousar, Abdul Wahid Baloch, Dina TabysAbstract:Abstract The N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) superfamily, specifically the SNAP25-type proteins and t-SNAREs, have been proposed to regulate cellular processes and plant resistance mechanisms. However, little is known about the role of SNAP25-type proteins in combating abiotic stresses, specifically in wild soybean. In the current study, the isolation and functional characterization of the putative synaptosomal-associated SNAP25-type protein gene GsSNAP33 from wild soybean ( Glycine Soja ) were performed. GsSNAP33 has a molecular weight of 33,311 Da and comprises 300 amino acid residues along with Qb-Qc SNARE domains. Multiple sequence alignment revealed the highest similarity of the GsSNAP33 protein to GmSNAP33 (91%), VrSNAP33 (89%), PvSNAP33 (86%) and AtSNAP33 (63%). Phylogenetic studies revealed the abundance of SNAP33 proteins mostly in dicotyledons. Quantitative real-time PCR assays confirmed that GsSNAP33 expression can be induced by salt, alkali, ABA and PEG treatments and that GsSNAP33 is highly expressed in the pods, seeds and roots of Glycine Soja . Furthermore, the overexpression of the GsSNAP33 gene in WT Arabidopsis thaliana resulted in increased germination rates, greater root lengths, improved photosynthesis, lower electrolyte leakage, higher biomass production and up-regulated expression levels of various stress-responsive marker genes, including KINI , COR15A , P5Cs , RAB18 , RD29A and COR47 in transgenic lines compared with those in WT lines. Subcellular localization studies revealed that the GsSNAP33-eGFP fusion protein was localized to the plasma membrane, while eGFP was distributed throughout whole cytoplasm of onion epidermal cells. Collectively, our findings suggest that GsSNAP33 , a novel plasma membrane protein gene of Glycine Soja , might be involved in improving plant responses to salt and drought stresses in Arabidopsis .
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A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.
PloS one, 2015Co-Authors: Xiaoli Sun, Chao Chen, Bowei Jia, Yang Shen, Mingzhe Sun, Zhiwei Qin, Kejun Yang, Zhang Meiping, Cong Mingyang, Yanming ZhuAbstract:It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine Soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine Soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.
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GsVAMP72, a novel Glycine Soja R-SNARE protein, is involved in regulating plant salt tolerance and ABA sensitivity
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Xiaoli Sun, Xi Bai, Hua Cai, Mingzhe Sun, Xiaodong Ding, Shanshan Yang, Xue Qian, Yanming ZhuAbstract:Abiotic stress, especially high salinity, is a major threat to agricultural production. It has been well established that SNARE proteins sustain directed vesicle traffic to underpin plant growth and development, yet little is known about the role of SNARE protein in the capacity to withstand abiotic stress, especially in wild soybeans. Here we identified and characterized a GsCBRLK interacting protein, GsVAMP72, which is a putative vesicle-associated membrane protein in Glycine Soja. GsVAMP72 protein has a longin domain at its N-terminus, belonging to R-SNARE family. Quantitative real-time (RT) PCR and beta-glucuronidase (GUS) activity assays revealed that the expression of GsVAMP72 was highly and rapidly induced by both high salt and ABA treatments. Overexpression of GsVAMP72 in Arabidopsis significantly reduced salt tolerance by modifying the ionic content and down-regulating expression of stress-responsive genes, including RD29A, COR47, KIN1, COR15A and RAB18. On the other hand, GsVAMP72 overexpression increased plant ABA sensitivity and altered the expression levels of ABA-responsive genes. Subcellular localization analysis showed that eGFP–GsVAMP72 fusion protein was observed on the plasma membrane-like and endosome-like structures but eGFP alone was distributing throughout the cytoplasm in Arabidopsis protoplasts and onion epidermal cells. GsVAMP72 promoter-controlled GUS activity was detected in both vegetative and reproductive organs, and was strongly induced by salt and ABA. In summary, we demonstrated that GsVAMP72 is a novel Glycine Soja vesicle-associated membrane protein and is highly involved in regulating plant responses to salt and ABA stresses.