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Jacek Baranowski - One of the best experts on this subject based on the ideXlab platform.
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UHPLC method for the simultaneous determination of β-blockers, isoflavones and their metabolites in human urine
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2011Co-Authors: Irena Baranowska, Sylwia Magiera, Jacek BaranowskiAbstract:A rapid-resolution ultra high-performance liquid chromatography separation method (UHPLC) for the simultaneous determination of the following β-blockers: milrinone, sotalol, metoprolol, propranolol and carvedilol, and their metabolites: 5′-hydroxylphenyl-carvedilol, O-desmethylcarvedilol, 4-hydroxypropranolol, α-hydroxy-metoprolol, O-desmethyl-metoprolol; the following isoflavones: genistein, daidzein, glycitin, Glycitein, puerarin and biochanin A; as well as their metabolites: dihydrogenistein, desmethylGlycitein, 8-hydroxygenistein, daidzein-7,4′-diglucoside, 8-hydroxydaidzein, dihydrobiochanin A in human urine was optimized. The analysed compounds were extracted from human urine by means of solid phase extraction (SPE). The effective UHPLC separation of the examined compounds was applied on a Hypersil GOLD™ (50 mm × 2.1 mm, 1.9μm) column with a gradient mobile phase system and a UV detector. The complete separation of all analytes was achieved within 8.0. min. The method was validated for the determination of the aforementioned substances in human urine. The linear ranges, limits of detection (LOD) and limits of quantification (LOQ) for β-blockers, isoflavones and their metabolites were determined. The intra- and inter-day precision (%C.V.) was less than 4.48%, and the intra-day and inter-day accuracy was less than 4.74%. The tested SPE sorbent proved that appropriate absolute recoveries can be obtained for Oasis HLB (Waters). The mean recovery of the analytes, using the new SPE procedure, amounted from 70.14% to 99.85%. The present paper reports, for the first time, the method for the determination of β-blockers, isoflavones and their metabolites in human urine samples. The newly developed method was suitably validated and successfully applied for the analysis of the certain of the aforementioned analytes in human urine samples obtained from the patients suffering cardiovascular disease. © 2011 Elsevier B.V.
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UHPLC method for the simultaneous determination of beta-blockers, isoflavones and their metabolites in human urine.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2011Co-Authors: Irena Baranowska, Sylwia Magiera, Jacek BaranowskiAbstract:A rapid-resolution ultra high-performance liquid chromatography separation method (UHPLC) for the simultaneous determination of the following beta-blockers: milrinone, sotalol, metoprolol, propranolol and carvedilol, and their metabolites: 5'-hydroxylphenyl-carvedilol, O-desmethylcarvedilol, 4-hydroxypropranolol, alpha-hydroxy-metoprolol, O-desmethyl-metoprolol; the following isoflavones: genistein, daidzein, glycitin, Glycitein, puerarin and biochanin A; as well as their metabolites: dihydrogenistein, desmethylGlycitein, 8-hydroxygenistein, daidzein-7,4'-diglucoside, 8-hydroxydaidzein, dihydrobiochanin A in human urine was optimized. The analysed compounds were extracted from human urine by means of solid phase extraction (SPE). The effective UHPLC separation of the examined compounds was applied on a Hypersil GOLD (50 mmx2.1 mm, 1.9 mum) column with a gradient mobile phase system and a UV detector. The complete separation of all analytes was achieved within 8.0 min. The method was validated for the determination of the aforementioned substances in human urine. The linear ranges, limits of detection (LOD) and limits of quantification (LOQ) for beta-blockers, isoflavones and their metabolites were determined. The intra- and inter-day precision (%C.V.) was less than 4.48%, and the intra-day and inter-day accuracy was less than 4.74%. The tested SPE sorbent proved that appropriate absolute recoveries can be obtained for Oasis HLB (Waters). The mean recovery of the analytes, using the new SPE procedure, amounted from 70.14% to 99.85%. The present paper reports, for the first time, the method for the determination of beta-blockers, isoflavones and their metabolites in human urine samples. The newly developed method was suitably validated and successfully applied for the analysis of the certain of the aforementioned analytes in human urine samples obtained from the patients suffering cardiovascular disease. Copyright 2011 Elsevier B.V. All rights reserved.
Suzanne Hendrich - One of the best experts on this subject based on the ideXlab platform.
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Bacteroides uniformis Is a Putative Bacterial Species Associated with the Degradation of the Isoflavone Genistein in Human Feces
Journal of Nutrition, 2011Co-Authors: Mathieu Renouf, Suzanne HendrichAbstract:: Inter-individual variation in isoflavone absorption depends on gut microbial degradation and affects the efficacy of these compounds. We hypothesized that inter-individual variation in fecal isoflavone disappearance coincided with variation in bacterial species. In vitro anaerobic fecal disappearance of isoflavones was measured from 33 participants by HPLC. Fecal microbial 16S rRNA variable region PCR products were obtained from 4 participants with the greatest and least genistein or Glycitein degradation and were subjected to denaturing gradient gel electrophoresis. DNA bands with a homology of 90-95% to Bacteroides uniformis and Faecalibacterium prausnitzii were present in greater intensities in fecal samples showing a genistein disappearance rate constant of 1.47 ± 0.14 h(-1) compared with those with a genistein disappearance rate constant of 0.15 ± 0.03 h(-1) (P < 0.05). Human fecal bacterial species with DNA sequences 90-100% homologous to Tannerella forsythensis and 4 other species were present in greater intensities in fecal samples showing a Glycitein disappearance rate constant of 0.57 ± 0.30 h(-1) compared with fecal samples with a Glycitein disappearance rate constant of 0.08 ± 0.03 h(-1) (P < 0.05). In high degraders, B. uniformis may be a candidate for genistein degradation and T. forsythensis for Glycitein degradation, based on fecal isoflavone degradation in the presence of these species. Bacteroides acidifaciens increased isoflavone disappearance in anaerobic human fecal incubations under nutrient-rich and -depleted conditions, suggesting this species as one responsible for the generally high degradation of isoflavones by humans. These fecal microbes are candidate biomarkers for interindividual variation in isoflavone uptake and efficacy.
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isoflavone Glycitein diminished plasma cholesterol in female golden syrian hamsters
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Mathieu Renouf, Patricia Aikins Murphy, Zhong Ye, Suzanne HendrichAbstract:The soybean isoflavones, daidzein, genistein, and Glycitein, were hypothesized to act as cholesterol-lowering components, separate from soy protein. Pure synthetic daidzein, genistein, or Glycitein (0.9 mmol/kg diet) or a casein-based control diet was fed to groups of 10 female Golden Syrian hamsters for 4 weeks. Hamsters fed Glycitein had significantly lower plasma total (by 15%) and non-HDL (by 24%) cholesterol compared with those fed casein (P daidzein > genistein (33.2%, 4.6%, 2.2%, respectively), with the apparent absorption of Glycitein significantly greater than that of the other isoflavones. These data suggest that Glycitein’s greater cholesterol-lowering effect was due to its grea...
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High Urinary Isoflavone Excretion Phenotype Decreases Plasma Cholesterol in Golden Syrian Hamsters Fed Soy Protein
Journal of Nutrition, 2006Co-Authors: Zhong Ye, Mathieu Renouf, Cathy Hauck, Patricia A. Murphy, Suzanne HendrichAbstract:Apparent absorption of isoflavones varies greatly among individuals but is relatively stable within an individual. We hypothesized that high urinary isoflavone excreters would show less plasma non-HDL cholesterol (non-HDL-C) than low isoflavone excreters after soy protein feeding. Fifty Golden Syrian hamsters were fed a high-fat/casein diet (n ¼ 10) or a high-fat/soy protein diet (n ¼ 40) for 4 wk. We identified 2 distinct urinary isoflavone excretion phenotypes based upon HPLC analysis of urinary Glycitein using a pairwise correlation plots analysis, or based upon total urinary isoflavone using a hierarchical cluster test. High isoflavone excreters showed greater urinary isoflavones (P , 0.05) than did low isoflavone excreters at wk 1 and 4. The low urinary Glycitein excretion phenotype was more stable than the high urinary Glycitein excretion phenotype by McNemar’s test. High urinary isoflavone excreters had significantly less non-HDL-C than did the low isoflavone excreters or casein-fed controls (P , 0.05). Plasma total and non-HDL-C were negatively correlated with urinary daidzein, Glycitein, and total isoflavone excretion (r ¼ 20.45 to 20.58, P , 0.05). Urinary isoflavone excretion phenotypes predicted the cholesterol-lowering efficacy of soy protein. Isoflavone absorbability, probably due to gut microbial ecology, is an important controllable variable in studies of effects of soy protein on blood lipids. J. Nutr. 136: 2773‐2778, 2006.
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metabolism of Glycitein 7 4 dihydroxy 6 methoxy isoflavone by human gut microflora
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Andrean L Simons, Mathieu Renouf, Suzanne Hendrich, Patricia Aikins MurphyAbstract:Gut microbial disappearance and metabolism of the soy isoflavone Glycitein, 7,4‘-dihydroxy-6-methoxyisoflavone, were investigated by incubating Glycitein anaerobically with feces from 12 human subjects. The subjects' ages ranged from 24 to 53 years with a body mass index (BMI) of 20.9−25.8 kg/m2 (mean BMI = 24.0 ± 1.1 kg/m2). Glycitein disappearance followed an apparent first-order rate loss. Fecal Glycitein disappearance rates for the subjects segregated into three different groups described as high (k = 0.67 ± 0.14/h), moderate (k = 0.34 ± 0.04/h), and low (k = 0.15 ± 0.07/h) Glycitein degraders (p 0.05). Four putative Glycitein metabolites, characterized by liquid chromatography−mass spectrometry (electrospray ionization using positive ionization mode), were dihydroGlycitein, dihydro-6,7,4‘-trihydroxyisoflavone, and 5‘-O-methyl-O-desmethylangolensin. Two subjects p...
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urinary disposition of the soybean isoflavones daidzein genistein and Glycitein differs among humans with moderate fecal isoflavone degradation activity
Journal of Nutrition, 1999Co-Authors: Yan Zhang, Patricia Aikins Murphy, Guijuan Wang, Tong T Song, Suzanne HendrichAbstract:: Glycitein metabolism was compared with other isoflavones to begin to understand the effect of this compound. Total isoflavones of 4.5 micromol/kg body weight from soymilk (high in genistein and daidzein) and soygerm (high in daidzein and Glycitein) was fed to seven women and seven men. To minimize interindividual variation, only subjects with moderate fecal isoflavone degradation rates (half-lives of daidzein and genistein were 15.7 and 8.9 h, respectively) were included. The average 48-h urinary excretion of Glycitein, daidzein and genistein was approximately 55, 46 and 29% of the dose ingested, respectively, which was significantly different from each other in men and women (P daidzein > Glycitein) (P < 0.05) in men and women, but plasma isoflavone concentrations after soygerm feeding did not parallel soygerm isoflavone concentrations in women because genistein and Glycitein did not differ from each other at 6 h after feeding. Six hours after soygerm dosing, plasma isoflavone concentrations paralleled soygerm isoflavone levels in men. Based on plasma isoflavone concentrations at 6 h after dosing, the bioavailabilities of daidzein and genistein were similar in men and women. At the high Glycitein dose (soygerm), plasma concentration at 24 h after dosing suggested a modest gender difference in Glycitein bioavailability.
Patricia Aikins Murphy - One of the best experts on this subject based on the ideXlab platform.
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isoflavone Glycitein diminished plasma cholesterol in female golden syrian hamsters
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Mathieu Renouf, Patricia Aikins Murphy, Zhong Ye, Suzanne HendrichAbstract:The soybean isoflavones, daidzein, genistein, and Glycitein, were hypothesized to act as cholesterol-lowering components, separate from soy protein. Pure synthetic daidzein, genistein, or Glycitein (0.9 mmol/kg diet) or a casein-based control diet was fed to groups of 10 female Golden Syrian hamsters for 4 weeks. Hamsters fed Glycitein had significantly lower plasma total (by 15%) and non-HDL (by 24%) cholesterol compared with those fed casein (P daidzein > genistein (33.2%, 4.6%, 2.2%, respectively), with the apparent absorption of Glycitein significantly greater than that of the other isoflavones. These data suggest that Glycitein’s greater cholesterol-lowering effect was due to its grea...
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metabolism of Glycitein 7 4 dihydroxy 6 methoxy isoflavone by human gut microflora
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Andrean L Simons, Mathieu Renouf, Suzanne Hendrich, Patricia Aikins MurphyAbstract:Gut microbial disappearance and metabolism of the soy isoflavone Glycitein, 7,4‘-dihydroxy-6-methoxyisoflavone, were investigated by incubating Glycitein anaerobically with feces from 12 human subjects. The subjects' ages ranged from 24 to 53 years with a body mass index (BMI) of 20.9−25.8 kg/m2 (mean BMI = 24.0 ± 1.1 kg/m2). Glycitein disappearance followed an apparent first-order rate loss. Fecal Glycitein disappearance rates for the subjects segregated into three different groups described as high (k = 0.67 ± 0.14/h), moderate (k = 0.34 ± 0.04/h), and low (k = 0.15 ± 0.07/h) Glycitein degraders (p 0.05). Four putative Glycitein metabolites, characterized by liquid chromatography−mass spectrometry (electrospray ionization using positive ionization mode), were dihydroGlycitein, dihydro-6,7,4‘-trihydroxyisoflavone, and 5‘-O-methyl-O-desmethylangolensin. Two subjects p...
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a simple synthesis of 7 4 dihydroxy 6 methoxyisoflavone Glycitein the third soybean isoflavone
Journal of Natural Products, 2003Co-Authors: Caroline Langatthoruwa, Tong T Song, Jiang Hu, And Andrean L Simons, Patricia Aikins MurphyAbstract:4-Methoxyresorcinol (3) was synthesized as the precursor for Glycitein (6) synthesis by the oxidation of 3-hydroxy-4-methoxybenzaldehyde (1) to the aryl formate with H2O2 and a catalytic amount of SeO2. Glycitein (6) was synthesized by cyclization of 2,4,4‘-trihydroxy-5-methoxydeoxybenzoin (5) with N,N-dimethylformamide, boron trifluoride diethyl ether, and methanesulfonyl chloride in a microwave oven.
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urinary disposition of the soybean isoflavones daidzein genistein and Glycitein differs among humans with moderate fecal isoflavone degradation activity
Journal of Nutrition, 1999Co-Authors: Yan Zhang, Patricia Aikins Murphy, Guijuan Wang, Tong T Song, Suzanne HendrichAbstract:: Glycitein metabolism was compared with other isoflavones to begin to understand the effect of this compound. Total isoflavones of 4.5 micromol/kg body weight from soymilk (high in genistein and daidzein) and soygerm (high in daidzein and Glycitein) was fed to seven women and seven men. To minimize interindividual variation, only subjects with moderate fecal isoflavone degradation rates (half-lives of daidzein and genistein were 15.7 and 8.9 h, respectively) were included. The average 48-h urinary excretion of Glycitein, daidzein and genistein was approximately 55, 46 and 29% of the dose ingested, respectively, which was significantly different from each other in men and women (P daidzein > Glycitein) (P < 0.05) in men and women, but plasma isoflavone concentrations after soygerm feeding did not parallel soygerm isoflavone concentrations in women because genistein and Glycitein did not differ from each other at 6 h after feeding. Six hours after soygerm dosing, plasma isoflavone concentrations paralleled soygerm isoflavone levels in men. Based on plasma isoflavone concentrations at 6 h after dosing, the bioavailabilities of daidzein and genistein were similar in men and women. At the high Glycitein dose (soygerm), plasma concentration at 24 h after dosing suggested a modest gender difference in Glycitein bioavailability.
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estrogenic activity of Glycitein a soy isoflavone
Journal of Agricultural and Food Chemistry, 1999Co-Authors: Tong T Song, Suzanne Hendrich, Patricia Aikins MurphyAbstract:Glycitein (4‘,7-dihydroxy-6-methoxyisoflavone) accounts for 5−10% of the total isoflavones in soy food products. The biological activity of this compound has not been reported to date, although numerous studies have been performed with the other soy isoflavones, daidzein and genistein. Glycitein was isolated from soy germ to 99% purity. Weaning female B6D2F1 mice were dosed with Glycitein (3 mg/day), genistein (3 mg/day), and diethylstilbestrol (DES) (0.03 μg/day) in 5% Tween 80 by gavage for 4 days. A control group received an equal volume of 5% Tween 80 solution daily. The uterine weight increased 150% with Glycitein (p < 0.001), 50% with genistein (p < 0.001), and 60% with DES (p < 0.001) compared with the control group. DES, 17β-estradiol, and three isoflavones (daidzein, genistein, and Glycitein) were examined for their competitive binding abilities with 17β-(3H)estradiol to the estrogen receptor proteins of the B6D2F1 mouse uterine cytosol. The concentrations of each compound required to displace 50...
Chih Yang Huang - One of the best experts on this subject based on the ideXlab platform.
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the p38 and nfκb signaling protein activation involved in Glycitein protective effects on isoproterenol treated h9c2 cardiomyoblast cells
Journal of Functional Foods, 2013Co-Authors: Tung Sheng Chen, Yi Hui Li, Fuu Jen Tsai, Chang Hai Tsai, Chih Yang HuangAbstract:Abstract Heart disease (HD) is greatly associated with gender and clinical evidence shows that increased serum norepinephrine levels are found in patients with HD. This study investigates the cardio-protective effect of Glycitein, a selective estrogen receptor modulator (SERM) from soy bean extract, on H9c2 cardiomyoblast cells treated with isoproterenol (ISO, a norepinephrine analog). The image data and results from western blotting showed that ISO treatment was capable of inducing cellular apoptosis, especially the mitochondrial dependent pathway. Glycitein treatment could suppress mitochondrial pro-apoptotic proteins expression including caspase-9 and caspase-3 in H9c2 treated with ISO. In contrast, several survival proteins were expressed in H9c2 cells treated with Glycitein, such as phosphor (p)-Akt, p-Bad and Akt. We confirmed that the protective role of Glycitein was partially mediated through the expression of p-38 and NFκB proteins by adding several pathway inhibitors.
Irena Baranowska - One of the best experts on this subject based on the ideXlab platform.
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UHPLC method for the simultaneous determination of β-blockers, isoflavones and their metabolites in human urine
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2011Co-Authors: Irena Baranowska, Sylwia Magiera, Jacek BaranowskiAbstract:A rapid-resolution ultra high-performance liquid chromatography separation method (UHPLC) for the simultaneous determination of the following β-blockers: milrinone, sotalol, metoprolol, propranolol and carvedilol, and their metabolites: 5′-hydroxylphenyl-carvedilol, O-desmethylcarvedilol, 4-hydroxypropranolol, α-hydroxy-metoprolol, O-desmethyl-metoprolol; the following isoflavones: genistein, daidzein, glycitin, Glycitein, puerarin and biochanin A; as well as their metabolites: dihydrogenistein, desmethylGlycitein, 8-hydroxygenistein, daidzein-7,4′-diglucoside, 8-hydroxydaidzein, dihydrobiochanin A in human urine was optimized. The analysed compounds were extracted from human urine by means of solid phase extraction (SPE). The effective UHPLC separation of the examined compounds was applied on a Hypersil GOLD™ (50 mm × 2.1 mm, 1.9μm) column with a gradient mobile phase system and a UV detector. The complete separation of all analytes was achieved within 8.0. min. The method was validated for the determination of the aforementioned substances in human urine. The linear ranges, limits of detection (LOD) and limits of quantification (LOQ) for β-blockers, isoflavones and their metabolites were determined. The intra- and inter-day precision (%C.V.) was less than 4.48%, and the intra-day and inter-day accuracy was less than 4.74%. The tested SPE sorbent proved that appropriate absolute recoveries can be obtained for Oasis HLB (Waters). The mean recovery of the analytes, using the new SPE procedure, amounted from 70.14% to 99.85%. The present paper reports, for the first time, the method for the determination of β-blockers, isoflavones and their metabolites in human urine samples. The newly developed method was suitably validated and successfully applied for the analysis of the certain of the aforementioned analytes in human urine samples obtained from the patients suffering cardiovascular disease. © 2011 Elsevier B.V.
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UHPLC method for the simultaneous determination of beta-blockers, isoflavones and their metabolites in human urine.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2011Co-Authors: Irena Baranowska, Sylwia Magiera, Jacek BaranowskiAbstract:A rapid-resolution ultra high-performance liquid chromatography separation method (UHPLC) for the simultaneous determination of the following beta-blockers: milrinone, sotalol, metoprolol, propranolol and carvedilol, and their metabolites: 5'-hydroxylphenyl-carvedilol, O-desmethylcarvedilol, 4-hydroxypropranolol, alpha-hydroxy-metoprolol, O-desmethyl-metoprolol; the following isoflavones: genistein, daidzein, glycitin, Glycitein, puerarin and biochanin A; as well as their metabolites: dihydrogenistein, desmethylGlycitein, 8-hydroxygenistein, daidzein-7,4'-diglucoside, 8-hydroxydaidzein, dihydrobiochanin A in human urine was optimized. The analysed compounds were extracted from human urine by means of solid phase extraction (SPE). The effective UHPLC separation of the examined compounds was applied on a Hypersil GOLD (50 mmx2.1 mm, 1.9 mum) column with a gradient mobile phase system and a UV detector. The complete separation of all analytes was achieved within 8.0 min. The method was validated for the determination of the aforementioned substances in human urine. The linear ranges, limits of detection (LOD) and limits of quantification (LOQ) for beta-blockers, isoflavones and their metabolites were determined. The intra- and inter-day precision (%C.V.) was less than 4.48%, and the intra-day and inter-day accuracy was less than 4.74%. The tested SPE sorbent proved that appropriate absolute recoveries can be obtained for Oasis HLB (Waters). The mean recovery of the analytes, using the new SPE procedure, amounted from 70.14% to 99.85%. The present paper reports, for the first time, the method for the determination of beta-blockers, isoflavones and their metabolites in human urine samples. The newly developed method was suitably validated and successfully applied for the analysis of the certain of the aforementioned analytes in human urine samples obtained from the patients suffering cardiovascular disease. Copyright 2011 Elsevier B.V. All rights reserved.