The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform
Xiaoqing Chen - One of the best experts on this subject based on the ideXlab platform.
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preparative isolation and analysis of alcohol dehydrogenase inhibitors from Glycyrrhiza Uralensis Root using ultrafiltration combined with high performance liquid chromatography and high speed countercurrent chromatography
IEEE Journal of Solid-state Circuits, 2014Co-Authors: Miao Chen, Xiaoqing ChenAbstract:A simple, rapid, and effective assay based on ultrafiltration combined with high-performance liquid chromatography and high-speed countercurrent chromatography was developed for screening and purifying alcohol dehydrogenase inhibitors from Glycyrrhiza Uralensis Root extract. Experiments were carried out to optimize binding conditions including alcohol dehydrogenase concentration, incubation time, temperature, and pH. By comparing the chromatograms, three compounds were found possessing alcohol dehydrogenase binding activity in Glycyrrhiza Uralensis Root. Under the target-guidance of ultrafiltration combined with the high-performance liquid chromatography experiment, liquiritin (1), isoliquiritin (2), and liquiritigenin (3) were separated by high-speed countercurrent chromatography using ethyl acetate/methanol/water (5:1:4) as the solvent system. The alcohol dehydrogenase inhibitory activities of these three isolated compounds were assessed; compound 2 showed strongest inhibitory activity with an IC50 of 8.95 μM. The results of the present study indicated that the combinative method using ultrafiltration, high-performance liquid chromatography and high-speed countercurrent chromatography could be widely applied for the rapid screening and isolation of enzyme inhibitors from complex mixtures.
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Selective fishing and analysis of xanthine oxidase binders from two Fabaceae species by coupling enzyme functionalized core–shell magnetic nanoparticles with HPLC–MS
Journal of Chromatography B, 2014Co-Authors: Huading Zhao, Jingang Yu, Xinyu Jiang, Xiaoqing ChenAbstract:Abstract Xanthine oxidase (XOD) immobilized core–shell magnetic silica (Fe3O4@SiO2-XOD) nanoparticles coupled with high performance liquid chromatography–mass spectrometry (HPLC–MS) was developed to fish out and analyze XOD binders from two Fabaceae species, Puerariae lobata flower and Glycyrrhiza Uralensis Root. The prepared Fe3O4@SiO2-XOD nanoparticles exhibited good specificity for XOD binders, better dispersion in aqueous solution and reusability than those of Fe3O4-XOD nanoparticles. The amount of XOD immobilized onto Fe3O4@SiO2 nanoparticles was 339.9 μg/mg and the activity of Fe3O4@SiO2-XOD nanoparticles remained 95% after ten times usage. The optimum conditions of selective fishing were optimized, and finally incubating pH was set at 7, incubating temperature at 25 °C and adsorption time at 30 min. Twelve XOD binders were successfully identified from ethyl acetate extract of P. lobata flower and G. Uralensis Root. The developed method provides a rapid, purposeful and effective way to identify active compounds from natural complex mixtures.
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Selective fishing and analysis of xanthine oxidase binders from two Fabaceae species by coupling enzyme functionalized core-shell magnetic nanoparticles with HPLC-MS.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2013Co-Authors: Huading Zhao, Jingang Yu, Xinyu Jiang, Xiaoqing ChenAbstract:Xanthine oxidase (XOD) immobilized core-shell magnetic silica (Fe3O4@SiO2-XOD) nanoparticles coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed to fish out and analyze XOD binders from two Fabaceae species, Puerariae lobata flower and Glycyrrhiza Uralensis Root. The prepared Fe3O4@SiO2-XOD nanoparticles exhibited good specificity for XOD binders, better dispersion in aqueous solution and reusability than those of Fe3O4-XOD nanoparticles. The amount of XOD immobilized onto Fe3O4@SiO2 nanoparticles was 339.9μg/mg and the activity of Fe3O4@SiO2-XOD nanoparticles remained 95% after ten times usage. The optimum conditions of selective fishing were optimized, and finally incubating pH was set at 7, incubating temperature at 25°C and adsorption time at 30min. Twelve XOD binders were successfully identified from ethyl acetate extract of P. lobata flower and G. Uralensis Root. The developed method provides a rapid, purposeful and effective way to identify active compounds from natural complex mixtures.
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analysis of tyrosinase binders from Glycyrrhiza Uralensis Root evaluation and comparison of tyrosinase immobilized magnetic fishing hplc and reverse ultrafiltration hplc
Journal of Chromatography B, 2013Co-Authors: Xiaoqing Chen, Mijun PengAbstract:Abstract Tyrosinase inhibitors play an important role in cosmetic products, food supplements and medicinal industries. In this study, a new facile method based on tyrosinase immobilized magnetic fishing (IMF) coupled with high performance liquid chromatography–diode array detector–tandem mass spectrometry (HPLC–DAD–MS/MS) was developed to screen and identify tyrosinase binders from Glycyrrhiza Uralensis Root. Experiments were carried out to select optimal immobilization and incubation conditions. The activity of immobilized tyrosinase retained 76.3% after ten consecutive cycles, and remained over 95% when stored at 4 °C for about two months. Eleven tyrosinase binders, including original compounds and transformation products were successfully screened and characterized from G. Uralensis Root, while dihydrodaidzein and pratensein were reported for the first time. Compared with ultrafiltration-HPLC assay, IMF-HPLC displayed many attractive advantages, including convenient solid–liquid separation ability, good stability, high reusability and activity, which indicated that IMF-HPLC had a broad applicability. The results indicated that IMF-HPLC method is a facile, effective and reproductive way to screen and identify active components from complex mixtures.
Mijun Peng - One of the best experts on this subject based on the ideXlab platform.
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analysis of tyrosinase binders from Glycyrrhiza Uralensis Root evaluation and comparison of tyrosinase immobilized magnetic fishing hplc and reverse ultrafiltration hplc
Journal of Chromatography B, 2013Co-Authors: Xiaoqing Chen, Mijun PengAbstract:Abstract Tyrosinase inhibitors play an important role in cosmetic products, food supplements and medicinal industries. In this study, a new facile method based on tyrosinase immobilized magnetic fishing (IMF) coupled with high performance liquid chromatography–diode array detector–tandem mass spectrometry (HPLC–DAD–MS/MS) was developed to screen and identify tyrosinase binders from Glycyrrhiza Uralensis Root. Experiments were carried out to select optimal immobilization and incubation conditions. The activity of immobilized tyrosinase retained 76.3% after ten consecutive cycles, and remained over 95% when stored at 4 °C for about two months. Eleven tyrosinase binders, including original compounds and transformation products were successfully screened and characterized from G. Uralensis Root, while dihydrodaidzein and pratensein were reported for the first time. Compared with ultrafiltration-HPLC assay, IMF-HPLC displayed many attractive advantages, including convenient solid–liquid separation ability, good stability, high reusability and activity, which indicated that IMF-HPLC had a broad applicability. The results indicated that IMF-HPLC method is a facile, effective and reproductive way to screen and identify active components from complex mixtures.
Wei Zhang - One of the best experts on this subject based on the ideXlab platform.
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ginseng panax quinquefolius and licorice Glycyrrhiza Uralensis Root extract combinations increase hepatocarcinoma cell hep g2 viability
Evidence-based Complementary and Alternative Medicine, 2011Co-Authors: David G Popovich, Wei ZhangAbstract:The combined cytoactive effects of American ginseng (Panax quinquefolius) and licorice (Glycyrrhiza Uralensis) Root extracts were investigated in a hepatocarcinoma cell line (Hep-G2). An isobolographic analysis was utilized to express the possibility of synergistic, additive or antagonistic interaction between the two extracts. Both ginseng and licorice Roots are widely utilized in traditional Chinese medicine preparations to treat a variety of ailments. However, the effect of the herbs in combination is currently unknown in cultured Hep-G2 cells. Ginseng (GE) and licorice (LE) extracts were both able to reduce cell viability. The LC50 values, after 72 h, were found to be 0.64 ± 0.02 mg/mL (GE) and 0.53 ± 0.02 mg/mL (LE). An isobologram was plotted, which included five theoretical LC50s calculated, based on the fixed fraction method of combination ginseng to licorice extracts to establish a line of additivity. All combinations of GE to LE (1/5, 1/3, 1/2, 2/3, 4/5) produced an effect on Hep-G2 cell viability but they were all found to be antagonistic. The LC50 of fractions 1/3, 1/2, 2/3 were 23%, 21% and 18% above the theoretical LC50. Lactate dehydrogenase release indicated that as the proportion of GE to LE increased beyond 50%, the influence on membrane permeability increased. Cell-cycle analysis showed a slight but significant arrest at the G1 phase of cell cycle for LE. Both GE and LE reduced Hep-G2 viability independently; however, the combinations of both extracts were found to have an antagonistic effect on cell viability and increased cultured Hep-G2 survival.
Zhong Li - One of the best experts on this subject based on the ideXlab platform.
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estrogenic activities of extracts of chinese licorice Glycyrrhiza Uralensis Root in mcf 7 breast cancer cells
The Journal of Steroid Biochemistry and Molecular Biology, 2009Co-Authors: Chunyan Hu, Juan Du, Baoqing Mo, Hong Qi, Xinru Wang, Shengai Ye, Zhong LiAbstract:Abstract Despite the wide use of Chinese licorice Root ( Glycyrrhiza Uralensis ) for the treatment of menopausal complaints, little is known on its potential estrogenic properties, and available information relative to its effects on cell proliferation is contradictory. In this study, the estrogenic properties of licorice Root were evaluated in vitro by use of several assays. The effects of increasing concentrations of a DMSO extract of licorice Root on the growth of MCF-7 breast cancer cells were biphasic. The extract showed an ER-dependent growth-promoting effect at low concentrations and an ER-independent anti-proliferative activity at high concentrations. In further experiments, licorice Root was sequentially extracted to yield four fractions: hexane, EtOAc, methanol and H 2 O. Only the EtOAc extract had effects on cell proliferation similar to the DMSO extract. The hexane extract had no effect on cell growth. In contrast, the methanol and water extracts showed an ER-independent, growth-promoting effect. Similar to its effects on cell proliferation, the EtOAc extract had a biphasic effect on S phase cell cycle distribution and the level of PCNA protein. This extract-induced transactivation of endogenous ERα in MCF-7 cells, supported by inducing down-regulation of ERα protein and mRNA levels, and up-regulation of ERα target genes pS2 and GREB1. These results suggest that the activity of licorice Root and the balance between increased risk for cancer and prevention of estrogen-dependent breast cancer may depend on the amount of dietary intake.
Noriaki Kawano - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of the safety and efficacy of Glycyrrhiza Uralensis Root extracts produced using artificial hydroponic and artificial hydroponic-field hybrid cultivation systems III: anti-allergic effects of hot water extracts on IgE-mediated immediate hy
Journal of Natural Medicines, 2020Co-Authors: Mitsuhiko Nose, Riho Tsutsui, Shinsuke Hisaka, Hiroshi Akiyama, Takayuki Inui, Noriaki Kawano, Shigeki Hayashi, Atsuyuki Hishida, Hiroyuki Fuchino, Tadashi KudoAbstract:To evaluate the safety and efficacy of Glycyrrhiza Uralensis Root extracts produced using artificial hydroponic and artificial hydroponic-field hybrid cultivation systems, we investigated anti-allergic action in mice using IgE-mediated immediate hypersensitivity. Hot water extracts obtained from the Roots of Glycyrrhiza Uralensis cultivated using two systems were orally administered at a dose of 100 mg/kg as glycyrrhizin (GL) and compared with the commercial crude drug, Glycyrrhizae Radix. Both the artificial hydroponic and artificial hydroponic-field hybrid cultivated Root extracts showed anti-allergic effects on IgE-mediated immediate hypersensitivity in mice, as did the commercial crude drugs. These results highlight the potential for artificially cultivated Roots of Glycyrrhiza Uralensis to be used as an alternative medicinal source.
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Evaluation of the safety and efficacy of Glycyrrhiza Uralensis Root extracts produced using artificial hydroponic-field hybrid cultivation systems II: comparison of serum concentration of glycyrrhetinic acid serum concentration in mice
Journal of Natural Medicines, 2019Co-Authors: Mitsuhiko Nose, Shinsuke Hisaka, Takayuki Inui, Noriaki Kawano, Shigeki Hayashi, Atsuyuki Hishida, Hiroyuki Fuchino, Kazuma Yamanaka, Nobuo Kawahara, Kayo YoshimatsuAbstract:To evaluate the safety and efficacy of Glycyrrhiza Uralensis Root produced using artificial hydroponic and artificial hydroponic-field hybrid cultivation systems, we investigated the pharmacokinetics of a major metabolite of glycyrrhizin (GL), glycyrrhetinic acid (GA). Hot water extracts obtained from the Roots of the artificial hydroponic-field hybrid cultivated Glycyrrhiza Uralensis were orally administered at a dose of 100 mg/kg as GL in mice and, compared with a commercial crude drug, Glycyrrhizae Radix. The temporal changes in serum GA concentration was found to depend on the GL concentration of the hot-water extracts. When hot-water extracts containing relatively high GL were administered, bimodal peaks appeared. In contrast, a broad single peak was detected when a hot-water extract containing relatively low GL content was administered. These tendencies in the serum GA concentration time course were observed for all samples, regardless of their derivation. Moreover, we compared the pharmacokinetic parameters and found that the C _max and AUC_0–48 values after oral administration of the extracts from Glycyrrhiza Uralensis Roots produced by the artificial cultivation system are within the range of variation for the commercial crude drugs. These results suggest the possibility that Roots of Glycyrrhiza Uralensis cultivated by the artificial hydroponic-field hybrid cultivation system can be used in addition to currently available commercial crude drugs produced from wild plant resources.
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Evaluation of the safety and efficacy of Glycyrrhiza Uralensis Root extracts produced using artificial hydroponic and artificial hydroponic-field hybrid cultivation systems
Journal of Natural Medicines, 2017Co-Authors: Hiroshi Akiyama, Mitsuhiko Nose, Shinsuke Hisaka, Takayuki Inui, Hiroyuki Fuchino, N. Ohtsuki, H. Takiguchi, A. Tada, N. Sugimoto, Noriaki KawanoAbstract:Glycyrrhiza Uralensis Roots used in this study were produced using novel cultivation systems, including artificial hydroponics and artificial hydroponic-field hybrid cultivation. The equivalency between G. Uralensis Root extracts produced by hydroponics and/or hybrid cultivation and a commercial Glycyrrhiza crude drug were evaluated for both safety and efficacy, and there were no significant differences in terms of mutagenicity on the Ames tests. The levels of cadmium and mercury in both hydroponic Roots and crude drugs were less than the limit of quantitation. Arsenic levels were lower in all hydroponic Roots than in the crude drug, whereas mean lead levels in the crude drug were not significantly different from those in the hydroponically cultivated G. Uralensis Roots. Both hydroponic and hybrid-cultivated Root extracts showed antiallergic activities against contact hypersensitivity that were similar to those of the crude drug extracts. These study results suggest that hydroponic and hybrid-cultivated Roots are equivalent in safety and efficacy to those of commercial crude drugs. Further studies are necessary before the Roots are applicable as replacements for the currently available commercial crude drugs produced from wild plant resources.