The Experts below are selected from a list of 181230 Experts worldwide ranked by ideXlab platform
Takehiko Kitamori - One of the best experts on this subject based on the ideXlab platform.
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation sys...
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level.
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Femtoliter Gradient Elution System for Liquid Chromatography Utilizing Extended Nanofluidics
2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level
Hisashi Shimizu - One of the best experts on this subject based on the ideXlab platform.
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation sys...
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level.
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Femtoliter Gradient Elution System for Liquid Chromatography Utilizing Extended Nanofluidics
2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level
Jerry F Butler - One of the best experts on this subject based on the ideXlab platform.
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effects of temperature on sarcophaga haemorrhoidalis diptera sarcophagidae development
Journal of Medical Entomology, 1998Co-Authors: Jason H Byrd, Jerry F ButlerAbstract:Growth curves were studied for the larva and pupa of Sarcophaga haemorrhoidalis (Fallen) under cyclic temperatures with means of 15.6, 21.1, 26.7, and 35°C, and a constant temperature of 25°C. Development from 1st instar to adult under all regimes ranged from 252 to 802 h. A constant temperature of 25°C produced a range of pupation times from 93 to 153 h, with adult emergence ranging from 252 to 502 h. A maximal preferential temperature of 30°C was determined for maggots by using a Gradient System. This study shows that S. haemorrhoidalis can be used as an indicator species when establishing a postmortem interval as long as the investigator accounts for the developmental variation prevalent within this species.
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effects of temperature on sarcophaga haemorrhoidalis diptera sarcophagidae development
Journal of Medical Entomology, 1998Co-Authors: Jason H Byrd, Jerry F ButlerAbstract:Growth curves were studied for the larva and pupa of Sarcophaga haemorrhoidalis (Fallen) under cyclic temperatures with means of 15.6, 21.1, 26.7, and 35 degrees C, and a constant temperature of 25 degrees C. Development from 1st instar to adult under all regimes ranged from 252 to 802 h. A constant temperature of 25 degrees C produced a range of pupation times from 93 to 153 h, with adult emergence ranging from 252 to 502 h. A maximal preferential temperature of 30 degrees C was determined for maggots by using a Gradient System. This study shows that S. haemorrhoidalis can be used as an indicator species when establishing a postmortem interval as long as the investigator accounts for the developmental variation prevalent within this species.
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effects of temperature on chrysomya rufifacies diptera calliphoridae development
Journal of Medical Entomology, 1997Co-Authors: Jason H Byrd, Jerry F ButlerAbstract:Growth curves were studied for the egg, larva, and pupa of Chrysomya rufifacies (Macquart) under mean cyclic temperatures of 15.6, 21.1, 26.7, and 35.0°C and a constant temperature of 25.0°C. Development from egg to adult under all regimes ranged from 190 to 598 h. A constant temperature of 25°C produced a range of pupation times from 134 to 162 h, with adult emergence ranging from 237 to 289 h. The maximal preferential temperature of 35.1°C was determined for maggots using a Gradient System. Highly predictable developmental time, minimal larval length variation, and low cohort variation emphasize the utility of this species in entomological-based postmortem interval determinations. Therefore, C. rufifacies should be of primary forensic importance when recovered alone or in conjunction with other species of Calliphoridae.
Kouto Toyoda - One of the best experts on this subject based on the ideXlab platform.
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation sys...
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level.
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Femtoliter Gradient Elution System for Liquid Chromatography Utilizing Extended Nanofluidics
2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level
Kazuma Mawatari - One of the best experts on this subject based on the ideXlab platform.
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation sys...
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femtoliter Gradient elution System for liquid chromatography utilizing extended nanofluidics
Analytical Chemistry, 2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level.
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Femtoliter Gradient Elution System for Liquid Chromatography Utilizing Extended Nanofluidics
2019Co-Authors: Hisashi Shimizu, Kouto Toyoda, Kazuma Mawatari, Shigeru Terabe, Takehiko KitamoriAbstract:A Gradient System was developed for the separation of proteins on a femtoliter scale utilizing nanofluidic channels. In the history of chromatography, miniaturization of the separation column has been important for efficient separation and downsizing of instruments. Previously, our group developed a small and highly efficient chromatography System utilizing nanofluidic channels, although a flexible design of the Gradient was difficult and separation of proteins was not achieved. Here, we propose a flexible Gradient System using standard HPLC pumps and an auxiliary mixer with a simple sample injection System. In contrast to our previous sample injection System using pressure balance, the System enables a femtoliter-scale sample injection which is compatible with Gradient elution using HPLC pumps. The System was carefully designed, verified for sample injection and Gradient elution, and finally applied to the separation of proteins from model and real samples. This femtoliter-scale, efficient separation System will contribute to omics studies at the single-cell level
