The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Daniel G Tenen - One of the best experts on this subject based on the ideXlab platform.
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absence of Granulocyte colony stimulating Factor signaling and neutrophil development in ccaat enhancer binding protein alpha deficient mice
Proceedings of the National Academy of Sciences of the United States of America, 1997Co-Authors: Donger Zhang, Pu Zhang, Naidy Wang, Christopher J Hetherington, Gretchen J Darlington, Daniel G TenenAbstract:Transcription Factors are master regulatory switches of differentiation, including the development of specific hematopoietic lineages from stem cells. Here we show that mice with targeted disruption of the CCAAT enhancer binding protein α gene (C/EBPα) demonstrate a selective block in differentiation of neutrophils. Mature neutrophils and eosinophils are not observed in the blood or fetal liver of mutant animals, while other hematopoietic lineages, including monocytes, are not affected. Instead, most of the white cells in the peripheral blood of mutant mice had the appearance of myeloid blasts. We also observed a selective loss of expression of a critical gene target of CCAAT enhancer binding protein α, the Granulocyte Colony-Stimulating Factor receptor. As a result, multipotential myeloid progenitors from the mutant fetal liver are unable to respond to Granulocyte Colony-Stimulating Factor signaling, although they are capable of forming Granulocyte–macrophage and macrophage colonies in methylcellulose in response to other growth Factors. Finally, we demonstrate that the lack of Granulocyte development results from a defect intrinsic to the hematopoietic system; transplanted fetal liver from mutant mice can reconstitute lymphoid but not neutrophilic cells in irradiated recipients. These studies suggest a model by which transcription Factors can direct the differentiation of multipotential precursors through activation of expression of a specific growth Factor receptor, allowing proliferation and differentiation in response to a specific extracellular signal. In addition, the c/ebpα−/− mice may be useful in understanding the mechanisms involved in acute myelogenous leukemia, in which a block in differentiation of myeloid precursors is a key feature of the disease.
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absence of Granulocyte colony stimulating Factor signaling and neutrophil development in ccaat enhancer binding protein α deficient mice
Proceedings of the National Academy of Sciences of the United States of America, 1997Co-Authors: Donger Zhang, Pu Zhang, Naidy Wang, Christopher J Hetherington, Gretchen J Darlington, Daniel G TenenAbstract:Transcription Factors are master regulatory switches of differentiation, including the development of specific hematopoietic lineages from stem cells. Here we show that mice with targeted disruption of the CCAAT enhancer binding protein α gene (C/EBPα) demonstrate a selective block in differentiation of neutrophils. Mature neutrophils and eosinophils are not observed in the blood or fetal liver of mutant animals, while other hematopoietic lineages, including monocytes, are not affected. Instead, most of the white cells in the peripheral blood of mutant mice had the appearance of myeloid blasts. We also observed a selective loss of expression of a critical gene target of CCAAT enhancer binding protein α, the Granulocyte Colony-Stimulating Factor receptor. As a result, multipotential myeloid progenitors from the mutant fetal liver are unable to respond to Granulocyte Colony-Stimulating Factor signaling, although they are capable of forming Granulocyte–macrophage and macrophage colonies in methylcellulose in response to other growth Factors. Finally, we demonstrate that the lack of Granulocyte development results from a defect intrinsic to the hematopoietic system; transplanted fetal liver from mutant mice can reconstitute lymphoid but not neutrophilic cells in irradiated recipients. These studies suggest a model by which transcription Factors can direct the differentiation of multipotential precursors through activation of expression of a specific growth Factor receptor, allowing proliferation and differentiation in response to a specific extracellular signal. In addition, the c/ebpα−/− mice may be useful in understanding the mechanisms involved in acute myelogenous leukemia, in which a block in differentiation of myeloid precursors is a key feature of the disease.
Miguel A Sanz - One of the best experts on this subject based on the ideXlab platform.
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follow up of healthy donors receiving Granulocyte colony stimulating Factor for peripheral blood progenitor cell mobilization and collection results of the spanish donor registry
Haematologica, 2008Co-Authors: Javier De La Rubia, Felipe De Arriba, Cristina Arbona, Maria Jesus Pascual, Concha Zamora, Andres Insunza, Dorleta Martinez, Carmen Paniagua, Miguel Angel Diaz, Miguel A SanzAbstract:Background Information about the long-term follow-up and safety of Granulocyte Colony-Stimulating Factor administration to healthy donors is limited. The aims of this study were to analyze the side effects of Granulocyte Colony-Stimulating Factor administration in donors included in a Spanish Registry of hematopoietic stem cell donors and to determine the long-term outcome of these donors.Design and Methods The Spanish National Donor Registry was developed to record the short- and long-term results of Granulocyte Colony-Stimulating Factor administration to mobilize peripheral blood progenitor cells in normal donors. To date, 1436 donors (771 males, 665 females) with a median age of 37 years (range, 1 to 74 years) have been registered. Granulocyte Colony-Stimulating Factor was the only cytokine administered. A baseline investigation was performed in every donor before Granulocyte Colony-Stimulating Factor administration and follow-up investigations (controls) were planned at 4 weeks and annually thereafter for up to 5 years after the mobilization.Results At least one of the scheduled controls was performed in 736 donors, while 320 donors have been followed for 2 years or more. The peripheral white blood cell count decreased significantly from 6.8×109/L at baseline to 5.9×109/L at 4 weeks after leukapheresis (p
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basal cd34 cell count predicts peripheral blood progenitor cell mobilization and collection in healthy donors after administration of Granulocyte colony stimulating Factor
Haematologica, 2004Co-Authors: Javier De La Rubia, P Marin, Andres Insunza, J I Lorenzo, M Torrabadella, Miguel A SanzAbstract:We analyzed Factors predicting CD34(+) cell mobilization and collection after Granulocyte Colony-Stimulating Factor (G-CSF) administration in 47 healthy donors. Basal CD34(+) cell count and sex were the two variables that significantly predicted a better CD34(+) cell mobilization, and greater age was the only variable associated with lower CD34+ cell yields.
Keiya Ozawa - One of the best experts on this subject based on the ideXlab platform.
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engraftment syndrome after autologous peripheral blood stem cell transplantation with high numbers of peripheral blood stem cells followed by Granulocyte colony stimulating Factor administration
Bone Marrow Transplantation, 2000Co-Authors: Chizuru Kawano, Kazuo Muroi, Ryoko Kuribara, Kiyohiko Hatake, Yuko Matsumoto, T. Ohtsuki, Keiya OzawaAbstract:Engraftment syndrome after autologous peripheral blood stem cell transplantation with high numbers of peripheral blood stem cells followed by Granulocyte Colony-Stimulating Factor administration
Marina Cavazzanacalvo - One of the best experts on this subject based on the ideXlab platform.
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first experience of autologous peripheral blood stem cell mobilization with biosimilar Granulocyte colony stimulating Factor
Advances in Therapy, 2011Co-Authors: Francois Lefrere, Annecolette Brignier, Caroline Elie, Jeanantoine Ribeil, Michael Bernimoulin, Charbel Aoun, Liliane Dal Cortivo, Richard Delarue, Olivier Hermine, Marina CavazzanacalvoAbstract:Introduction Mobilization techniques for autologous peripheral blood stem cell (PBSC) collection include chemotherapy followed by hematopoietic growth Factors, such as Granulocyte Colony-Stimulating Factor (G-CSF). Biosimilar versions of G-CSF are now available in Europe.
Andrew S. Artz - One of the best experts on this subject based on the ideXlab platform.
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older age but not donor health impairs allogeneic Granulocyte colony stimulating Factor g csf peripheral blood stem cell mobilization
Biology of Blood and Marrow Transplantation, 2009Co-Authors: Elie Richa, Koen Van Besien, Mona Papari, Joann Allen, Guadalupe Martinez, Amittha Wickrema, John Anastasi, Andrew S. ArtzAbstract:We evaluated stem cell mobilization in 195 consecutive sibling donors who underwent a uniform mobilization regimen of Granulocyte Colony-Stimulating Factor (G-CSF) at 10μg/kg/day divided into twice daily dosing. On day 5, peripheral blood (PB) CD34 cells/μL were measured immediately prior to peripheral blood stem cell (PBSC) apheresis. Failed mobilization was defined as
