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Daniel Araki Ribeiro - One of the best experts on this subject based on the ideXlab platform.
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Chemopreventive activity of Grape Juice concentrate (G8000TM) on rat colon carcinogenesis induced by azoxymethane.
Environmental toxicology and pharmacology, 2015Co-Authors: Roseane Mendes Silva, Daniel Araki Ribeiro, Vanessa Maria De Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Ricardo Artigiani Neto, Celina Tizuko Fujiyama Oshima, Nora Manoukian ForonesAbstract:Colorectal cancer is the third most common cancer worldwide in both sexes, with similar geographic patterns between genders. This neoplasm has good prognosis if the disease is diagnosed at early stages. The aim of this study was to evaluate the effect of red Grape Juice on the expression of COX-2 and Ki-67 expression following colon carcinogenesis induced by azoxymethane (AOM). Thirty-five rats were randomly distributed into seven groups (n=5 per group): G1: SHAM or negative control received only saline; G2 (positive control): animals received 15 mg/kg AOM; G3: animals received 1% red Grape Juice 2 weeks before the administration of AOM; G4: animals received 2% red Grape Juice 2 weeks before the administration of AOM; G5: animals received 1% red Grape Juice 4 weeks after the last administration of AOM; G6: animals received 2% red Grape Juice 4 weeks after the last administration of AOM; G7: animals received only 2% red Grape Juice. COX-2 mRNA expression was reduced in animals treated with 1% red Grape Juice before AOM induction or 2% red Grape Juice after AOM induction. COX-2 immunoexpression was also reduced to groups treated with red Grape Juice at 1% before and after AOM induction or 2% red Grape Juice after AOM induction. Decreased immunoexpression of Ki-67 positive cells was observed in animals treated with 1% Grape Juice before AOM-treated animals. Taken together, Grape Juice concentrate is able to exert some chemopreventive activity on rat colon carcinogenesis.
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Grape Juice concentrate (G8000™) modulates apoptosis but not oxidative stress following rat colon carcinogenesis induced by azoxymethane
Toxicology Mechanisms and Methods, 2015Co-Authors: Celina Tizuko Fujiyama Oshima, Andréa Pittelli Boiago Gollücke, Daniel Araki Ribeiro, Roseane Mendes Silva, Vanessa Maria De Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Ricardo Artigiani Neto, Gilles Landman, Nora Manoukian ForonesAbstract:AbstractObjectives: The aim of this study was to evaluate if Grape Juice concentrate is able to protect against experimental colon carcinogenesis.Material and methods: For this purpose, a total of 35 male Wistar rats were randomly distributed into seven groups: G1: SHAM animals receiving only saline; G2: animals receiving 15 mg/kg azoxymethane (AOM); G3: animals receiving 1% Grape Juice concentrate 2 weeks before the administration of AOM; G4: animals receiving 2% Grape Juice concentrate 2 weeks before the administration of AOM; G5: animals receiving 1% Grape Juice concentrate 4 weeks after the last administration of AOM; G6: animals receiving 2% Grape Juice concentrate 4 weeks after the last administration of AOM; G7: animals receiving only 2% Grape Juice concentrate.Results: The group that received 2% Grape Juice concentrate before induction with AOM showed the decreased expression of Bcl-2 compared to those animals that were induced by AOM (positive control). Regarding Bax, animals that received Grape ...
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Antimutagenic and antigenotoxic potential of Grape Juice concentrate in blood and liver of rats exposed to cadmium.
Environmental Science and Pollution Research, 2014Co-Authors: Carolina Foot Gomes De Moura, Odair Aguiar, Andréa Pittelli Boiago Gollücke, Celina Tizuko Fujiyama Oshima, Victor Hugo Pereira Da Silva, Flávia Andressa Pidone Ribeiro, Gustavo Protasio Pacheco De Jesus, Daniel Araki RibeiroAbstract:The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of Grape Juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n = 5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-Grape Juice group (Cd + GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd + GJ group received Grape Juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg−1 day−1). Grape Juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with Grape Juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd + GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that Grape Juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.
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Grape Juice concentrate protects reproductive parameters of male rats against cadmium induced damage a chronic assay
British Journal of Nutrition, 2013Co-Authors: Vanessa Cardoso Pires, Andréa Pittelli Boiago Gollücke, Daniel Araki Ribeiro, Lisandro Lungato, Vânia Dalmeida, Odai AguiaAbstract:The aim of the present study was to investigate the effects of long-term Grape Juice concentrate (GJC) consumption, in two dosages, on the reproductive parameters of cadmium-exposed male rats. The effects of the concentrate on body mass gain, plasma testosterone levels, reproductive organ weights, daily sperm production, sperm morphology, testis histopathological and histomorphometrical parameters, and testicular antioxidant markers were investigated. Wistar rats ( n 54) were distributed into six groups: CdCl 2 ; cadmium and Grape Juice I (1·18 g/kg per d); cadmium and Grape Juice II (2·36 g/kg per d); Grape Juice I (1·18 g/kg per d); Grape Juice II (2·36 g/kg per d); control. A single dose of CdCl 2 (1·2 mg/kg body weight (BW)) was injected intraperitoneally and the Grape Juice was administered orally for 56 d. The results indicated that cadmium changed all reproductive and antioxidant parameters. At dosage I (1·18 g/kg BW), GJC consumption did not show the effects against cadmium-induced damages. In contrast, at dosage II (2·36 g/kg BW), the GJC improved the gonadosomatic index ( P = 0·003), serum testosterone levels ( P = 0·001), the relative weight of epididymis ( P = 0·013) and ventral prostate ( P = 0·052), the percentage of normal sperm ( P = 0·001), and histopathological and histomorphometrical parameters. In addition, at this dosage, normalisation of the enzymatic activity of superoxide dismutase ( P = 0·001) and of testicular levels of glutathione ( P = 0·03) were observed. The parameters of the non-exposed rats did not depict significant alterations. In conclusion, the product was able to act as a protector of reproductive function against cadmium-induced damage. Such a property was expressed in a dose-dependent manner as the more effective dose was dosage II. The GJC acted possibly by antioxidant mechanisms.
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the anti inflammatory potential of phenolic compounds in Grape Juice concentrate g8000 on 2 4 6 trinitrobenzene sulphonic acid induced colitis
British Journal of Nutrition, 2013Co-Authors: Ana Paula Ribeiro Paiotti, Andréa Pittelli Boiago Gollücke, Roseane Mendes Silva, Ricardo Artigiani Neto, Juliana Noguti, Patricia Marchi, Vanessa Pazine, Mauricio Mercaldi Pastrelo, Sender Jankiel Miszpute, Daniel Araki RibeiroAbstract:Chronic inflammatory bowel disease is characterised by an up-regulation of the synthesis and release of a variety of pro-inflammatory mediators leading to excessive tissue injury. Flavonoids are able to inhibit enzymes and/or due to their antioxidant properties regulate the immune response. The goal of the present study was to evaluate the mechanisms of action of phenolic compounds present in Grape Juice on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. A total of forty-one male Wistar rats were randomised into seven groups: negative control group; TNBS non-treated induced colitis; 2% Grape Juice control group; 1% Grape Juice 24 h after TNBS colitis induction; 1% Grape Juice on day 7 after colitis induction; 2% Grape Juice 24 h after colitis induction; 2% Grape Juice on day 7 after colitis induction. The 1% Grape Juice-treated induced colitis group showed marked clinical improvement when compared with the TNBS-induced colitis group. Rats that received 1% Grape Juice, on day 7 after colitis induction, presented reduced intensity of macroscopic and histological scores. Statistically significant differences (P,0·05) of TNF-a and inducible NO synthase mRNA expression were detected in the groups treated with Grape Juice at the 1% dose after inducing experimental colitis when compared with the TNBS group. Grape Juice reduced the noxious effects induced by colitis caused by TNBS, especially at the 1% dose.
A W Watson - One of the best experts on this subject based on the ideXlab platform.
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Cognitive and mood improvements following acute supplementation with purple Grape Juice in healthy young adults
European Journal of Nutrition, 2017Co-Authors: C. F. Haskell-ramsay, R.c. Stuart, Edward J. Okello, A W WatsonAbstract:PurposeBerry-derived phenolic compounds found in Grapes have been associated with a number of health benefits, including the augmentation of human brain function and cognition. Previous intervention studies of Concord Grape Juice have demonstrated improvement to memory and driving ability following 3- to 4-month supplementation in middle-aged and older adults. However, no studies to date have demonstrated acute cognitive benefits of Grape Juice, and investigation of these effects in young adults is lacking.MethodsThis randomised, placebo-controlled, double-blind, counterbalanced-crossover study, assessed the effects of 230 ml purple Grape Juice or sugar-matched control in 20 healthy young adults. Computerised measures of episodic memory, working memory, attention and mood were completed at baseline and following a 20-min absorption period.ResultsPurple Grape Juice significantly improved reaction time on a composite attention measure (p = 0.047) and increased calm ratings (p = 0.046) when compared to placebo. Order effects also indicated an enduring positive effect on pre-dose memory reaction time (p = 0.018) and post-dose calm ratings (p = 0.019) when purple Grape was consumed first.ConclusionsThese findings in a small sample of healthy young adults suggest that purple Grape Juice can acutely enhance aspects of cognition and mood. No significant effects of Juice were observed on memory measures, suggesting that these may be less susceptible to manipulation following acute supplementation in healthy young adults. Potential mechanisms underlying these effects include modulation of cerebral blood flow, glucoregulation and inhibition of monoamine oxidase activity, all of which require further exploration.
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cognitive and mood improvements following acute supplementation with purple Grape Juice in healthy young adults
European Journal of Nutrition, 2017Co-Authors: Crystal F Haskellramsay, R.c. Stuart, Edward J. Okello, A W WatsonAbstract:Purpose Berry-derived phenolic compounds found in Grapes have been associated with a number of health benefits, including the augmentation of human brain function and cognition. Previous intervention studies of Concord Grape Juice have demonstrated improvement to memory and driving ability following 3- to 4-month supplementation in middle-aged and older adults. However, no studies to date have demonstrated acute cognitive benefits of Grape Juice, and investigation of these effects in young adults is lacking.
Nora Manoukian Forones - One of the best experts on this subject based on the ideXlab platform.
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Chemopreventive activity of Grape Juice concentrate (G8000TM) on rat colon carcinogenesis induced by azoxymethane.
Environmental toxicology and pharmacology, 2015Co-Authors: Roseane Mendes Silva, Daniel Araki Ribeiro, Vanessa Maria De Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Ricardo Artigiani Neto, Celina Tizuko Fujiyama Oshima, Nora Manoukian ForonesAbstract:Colorectal cancer is the third most common cancer worldwide in both sexes, with similar geographic patterns between genders. This neoplasm has good prognosis if the disease is diagnosed at early stages. The aim of this study was to evaluate the effect of red Grape Juice on the expression of COX-2 and Ki-67 expression following colon carcinogenesis induced by azoxymethane (AOM). Thirty-five rats were randomly distributed into seven groups (n=5 per group): G1: SHAM or negative control received only saline; G2 (positive control): animals received 15 mg/kg AOM; G3: animals received 1% red Grape Juice 2 weeks before the administration of AOM; G4: animals received 2% red Grape Juice 2 weeks before the administration of AOM; G5: animals received 1% red Grape Juice 4 weeks after the last administration of AOM; G6: animals received 2% red Grape Juice 4 weeks after the last administration of AOM; G7: animals received only 2% red Grape Juice. COX-2 mRNA expression was reduced in animals treated with 1% red Grape Juice before AOM induction or 2% red Grape Juice after AOM induction. COX-2 immunoexpression was also reduced to groups treated with red Grape Juice at 1% before and after AOM induction or 2% red Grape Juice after AOM induction. Decreased immunoexpression of Ki-67 positive cells was observed in animals treated with 1% Grape Juice before AOM-treated animals. Taken together, Grape Juice concentrate is able to exert some chemopreventive activity on rat colon carcinogenesis.
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Grape Juice concentrate (G8000™) modulates apoptosis but not oxidative stress following rat colon carcinogenesis induced by azoxymethane
Toxicology Mechanisms and Methods, 2015Co-Authors: Celina Tizuko Fujiyama Oshima, Andréa Pittelli Boiago Gollücke, Daniel Araki Ribeiro, Roseane Mendes Silva, Vanessa Maria De Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Ricardo Artigiani Neto, Gilles Landman, Nora Manoukian ForonesAbstract:AbstractObjectives: The aim of this study was to evaluate if Grape Juice concentrate is able to protect against experimental colon carcinogenesis.Material and methods: For this purpose, a total of 35 male Wistar rats were randomly distributed into seven groups: G1: SHAM animals receiving only saline; G2: animals receiving 15 mg/kg azoxymethane (AOM); G3: animals receiving 1% Grape Juice concentrate 2 weeks before the administration of AOM; G4: animals receiving 2% Grape Juice concentrate 2 weeks before the administration of AOM; G5: animals receiving 1% Grape Juice concentrate 4 weeks after the last administration of AOM; G6: animals receiving 2% Grape Juice concentrate 4 weeks after the last administration of AOM; G7: animals receiving only 2% Grape Juice concentrate.Results: The group that received 2% Grape Juice concentrate before induction with AOM showed the decreased expression of Bcl-2 compared to those animals that were induced by AOM (positive control). Regarding Bax, animals that received Grape ...
Roseane Mendes Silva - One of the best experts on this subject based on the ideXlab platform.
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Chemopreventive activity of Grape Juice concentrate (G8000TM) on rat colon carcinogenesis induced by azoxymethane.
Environmental toxicology and pharmacology, 2015Co-Authors: Roseane Mendes Silva, Daniel Araki Ribeiro, Vanessa Maria De Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Ricardo Artigiani Neto, Celina Tizuko Fujiyama Oshima, Nora Manoukian ForonesAbstract:Colorectal cancer is the third most common cancer worldwide in both sexes, with similar geographic patterns between genders. This neoplasm has good prognosis if the disease is diagnosed at early stages. The aim of this study was to evaluate the effect of red Grape Juice on the expression of COX-2 and Ki-67 expression following colon carcinogenesis induced by azoxymethane (AOM). Thirty-five rats were randomly distributed into seven groups (n=5 per group): G1: SHAM or negative control received only saline; G2 (positive control): animals received 15 mg/kg AOM; G3: animals received 1% red Grape Juice 2 weeks before the administration of AOM; G4: animals received 2% red Grape Juice 2 weeks before the administration of AOM; G5: animals received 1% red Grape Juice 4 weeks after the last administration of AOM; G6: animals received 2% red Grape Juice 4 weeks after the last administration of AOM; G7: animals received only 2% red Grape Juice. COX-2 mRNA expression was reduced in animals treated with 1% red Grape Juice before AOM induction or 2% red Grape Juice after AOM induction. COX-2 immunoexpression was also reduced to groups treated with red Grape Juice at 1% before and after AOM induction or 2% red Grape Juice after AOM induction. Decreased immunoexpression of Ki-67 positive cells was observed in animals treated with 1% Grape Juice before AOM-treated animals. Taken together, Grape Juice concentrate is able to exert some chemopreventive activity on rat colon carcinogenesis.
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Grape Juice concentrate (G8000™) modulates apoptosis but not oxidative stress following rat colon carcinogenesis induced by azoxymethane
Toxicology Mechanisms and Methods, 2015Co-Authors: Celina Tizuko Fujiyama Oshima, Andréa Pittelli Boiago Gollücke, Daniel Araki Ribeiro, Roseane Mendes Silva, Vanessa Maria De Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Ricardo Artigiani Neto, Gilles Landman, Nora Manoukian ForonesAbstract:AbstractObjectives: The aim of this study was to evaluate if Grape Juice concentrate is able to protect against experimental colon carcinogenesis.Material and methods: For this purpose, a total of 35 male Wistar rats were randomly distributed into seven groups: G1: SHAM animals receiving only saline; G2: animals receiving 15 mg/kg azoxymethane (AOM); G3: animals receiving 1% Grape Juice concentrate 2 weeks before the administration of AOM; G4: animals receiving 2% Grape Juice concentrate 2 weeks before the administration of AOM; G5: animals receiving 1% Grape Juice concentrate 4 weeks after the last administration of AOM; G6: animals receiving 2% Grape Juice concentrate 4 weeks after the last administration of AOM; G7: animals receiving only 2% Grape Juice concentrate.Results: The group that received 2% Grape Juice concentrate before induction with AOM showed the decreased expression of Bcl-2 compared to those animals that were induced by AOM (positive control). Regarding Bax, animals that received Grape ...
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the anti inflammatory potential of phenolic compounds in Grape Juice concentrate g8000 on 2 4 6 trinitrobenzene sulphonic acid induced colitis
British Journal of Nutrition, 2013Co-Authors: Ana Paula Ribeiro Paiotti, Andréa Pittelli Boiago Gollücke, Roseane Mendes Silva, Ricardo Artigiani Neto, Juliana Noguti, Patricia Marchi, Vanessa Pazine, Mauricio Mercaldi Pastrelo, Sender Jankiel Miszpute, Daniel Araki RibeiroAbstract:Chronic inflammatory bowel disease is characterised by an up-regulation of the synthesis and release of a variety of pro-inflammatory mediators leading to excessive tissue injury. Flavonoids are able to inhibit enzymes and/or due to their antioxidant properties regulate the immune response. The goal of the present study was to evaluate the mechanisms of action of phenolic compounds present in Grape Juice on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. A total of forty-one male Wistar rats were randomised into seven groups: negative control group; TNBS non-treated induced colitis; 2% Grape Juice control group; 1% Grape Juice 24 h after TNBS colitis induction; 1% Grape Juice on day 7 after colitis induction; 2% Grape Juice 24 h after colitis induction; 2% Grape Juice on day 7 after colitis induction. The 1% Grape Juice-treated induced colitis group showed marked clinical improvement when compared with the TNBS-induced colitis group. Rats that received 1% Grape Juice, on day 7 after colitis induction, presented reduced intensity of macroscopic and histological scores. Statistically significant differences (P,0·05) of TNF-a and inducible NO synthase mRNA expression were detected in the groups treated with Grape Juice at the 1% dose after inducing experimental colitis when compared with the TNBS group. Grape Juice reduced the noxious effects induced by colitis caused by TNBS, especially at the 1% dose.
Andréa Pittelli Boiago Gollücke - One of the best experts on this subject based on the ideXlab platform.
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Grape Juice concentrate (G8000™) modulates apoptosis but not oxidative stress following rat colon carcinogenesis induced by azoxymethane
Toxicology Mechanisms and Methods, 2015Co-Authors: Celina Tizuko Fujiyama Oshima, Andréa Pittelli Boiago Gollücke, Daniel Araki Ribeiro, Roseane Mendes Silva, Vanessa Maria De Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Ricardo Artigiani Neto, Gilles Landman, Nora Manoukian ForonesAbstract:AbstractObjectives: The aim of this study was to evaluate if Grape Juice concentrate is able to protect against experimental colon carcinogenesis.Material and methods: For this purpose, a total of 35 male Wistar rats were randomly distributed into seven groups: G1: SHAM animals receiving only saline; G2: animals receiving 15 mg/kg azoxymethane (AOM); G3: animals receiving 1% Grape Juice concentrate 2 weeks before the administration of AOM; G4: animals receiving 2% Grape Juice concentrate 2 weeks before the administration of AOM; G5: animals receiving 1% Grape Juice concentrate 4 weeks after the last administration of AOM; G6: animals receiving 2% Grape Juice concentrate 4 weeks after the last administration of AOM; G7: animals receiving only 2% Grape Juice concentrate.Results: The group that received 2% Grape Juice concentrate before induction with AOM showed the decreased expression of Bcl-2 compared to those animals that were induced by AOM (positive control). Regarding Bax, animals that received Grape ...
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Antimutagenic and antigenotoxic potential of Grape Juice concentrate in blood and liver of rats exposed to cadmium.
Environmental Science and Pollution Research, 2014Co-Authors: Carolina Foot Gomes De Moura, Odair Aguiar, Andréa Pittelli Boiago Gollücke, Celina Tizuko Fujiyama Oshima, Victor Hugo Pereira Da Silva, Flávia Andressa Pidone Ribeiro, Gustavo Protasio Pacheco De Jesus, Daniel Araki RibeiroAbstract:The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of Grape Juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n = 5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-Grape Juice group (Cd + GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd + GJ group received Grape Juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg−1 day−1). Grape Juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with Grape Juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd + GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that Grape Juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.
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Grape Juice concentrate protects reproductive parameters of male rats against cadmium induced damage a chronic assay
British Journal of Nutrition, 2013Co-Authors: Vanessa Cardoso Pires, Andréa Pittelli Boiago Gollücke, Daniel Araki Ribeiro, Lisandro Lungato, Vânia Dalmeida, Odai AguiaAbstract:The aim of the present study was to investigate the effects of long-term Grape Juice concentrate (GJC) consumption, in two dosages, on the reproductive parameters of cadmium-exposed male rats. The effects of the concentrate on body mass gain, plasma testosterone levels, reproductive organ weights, daily sperm production, sperm morphology, testis histopathological and histomorphometrical parameters, and testicular antioxidant markers were investigated. Wistar rats ( n 54) were distributed into six groups: CdCl 2 ; cadmium and Grape Juice I (1·18 g/kg per d); cadmium and Grape Juice II (2·36 g/kg per d); Grape Juice I (1·18 g/kg per d); Grape Juice II (2·36 g/kg per d); control. A single dose of CdCl 2 (1·2 mg/kg body weight (BW)) was injected intraperitoneally and the Grape Juice was administered orally for 56 d. The results indicated that cadmium changed all reproductive and antioxidant parameters. At dosage I (1·18 g/kg BW), GJC consumption did not show the effects against cadmium-induced damages. In contrast, at dosage II (2·36 g/kg BW), the GJC improved the gonadosomatic index ( P = 0·003), serum testosterone levels ( P = 0·001), the relative weight of epididymis ( P = 0·013) and ventral prostate ( P = 0·052), the percentage of normal sperm ( P = 0·001), and histopathological and histomorphometrical parameters. In addition, at this dosage, normalisation of the enzymatic activity of superoxide dismutase ( P = 0·001) and of testicular levels of glutathione ( P = 0·03) were observed. The parameters of the non-exposed rats did not depict significant alterations. In conclusion, the product was able to act as a protector of reproductive function against cadmium-induced damage. Such a property was expressed in a dose-dependent manner as the more effective dose was dosage II. The GJC acted possibly by antioxidant mechanisms.
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the anti inflammatory potential of phenolic compounds in Grape Juice concentrate g8000 on 2 4 6 trinitrobenzene sulphonic acid induced colitis
British Journal of Nutrition, 2013Co-Authors: Ana Paula Ribeiro Paiotti, Andréa Pittelli Boiago Gollücke, Roseane Mendes Silva, Ricardo Artigiani Neto, Juliana Noguti, Patricia Marchi, Vanessa Pazine, Mauricio Mercaldi Pastrelo, Sender Jankiel Miszpute, Daniel Araki RibeiroAbstract:Chronic inflammatory bowel disease is characterised by an up-regulation of the synthesis and release of a variety of pro-inflammatory mediators leading to excessive tissue injury. Flavonoids are able to inhibit enzymes and/or due to their antioxidant properties regulate the immune response. The goal of the present study was to evaluate the mechanisms of action of phenolic compounds present in Grape Juice on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. A total of forty-one male Wistar rats were randomised into seven groups: negative control group; TNBS non-treated induced colitis; 2% Grape Juice control group; 1% Grape Juice 24 h after TNBS colitis induction; 1% Grape Juice on day 7 after colitis induction; 2% Grape Juice 24 h after colitis induction; 2% Grape Juice on day 7 after colitis induction. The 1% Grape Juice-treated induced colitis group showed marked clinical improvement when compared with the TNBS-induced colitis group. Rats that received 1% Grape Juice, on day 7 after colitis induction, presented reduced intensity of macroscopic and histological scores. Statistically significant differences (P,0·05) of TNF-a and inducible NO synthase mRNA expression were detected in the groups treated with Grape Juice at the 1% dose after inducing experimental colitis when compared with the TNBS group. Grape Juice reduced the noxious effects induced by colitis caused by TNBS, especially at the 1% dose.
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Grape Juice concentrate modulates p16 expression in high fat diet-induced liver steatosis in Wistar rats
Toxicology Mechanisms and Methods, 2012Co-Authors: Andressa Orlandeli Ferreira, Andréa Pittelli Boiago Gollücke, Juliana Noguti, Victor Hugo Pereira Da Silva, Elsa Tiemi Hojo Yamamura, Daniel Araki RibeiroAbstract:Purpose: The goal of this study was to investigate whether subchronic treatment with Grape Juice concentrate is able to protect the liver from high fat diet injury in rats. The effects of Grape Juice concentrate treatment on histopathological changes, and immunohistochemistry for p53, p16 and p21 were evaluated.Methods: Male Wistar rats (n = 18) were distributed into three groups: group 1: negative control; group 2: cholesterol at 1% (w/w) in their diet, treated during 5 weeks; and group 3: cholesterol at 1% in their chow during 5 weeks, and Grape Juice concentrate at 222 mg per day in their drinking-water in the last week only.Results: The results pointed out that treatment with Grape Juice concentrate did not show remarkable differences regarding liver tissue in the cholesterol-exposed group when compared to group 2. However, Grape Juice concentrate was able to modulate p16 immunoexpression when compared to high fat diet group. p53 and p21 did not show any significant statistical differences among group...
