The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Gao-xue Wang - One of the best experts on this subject based on the ideXlab platform.
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Antigen epitope screening of Grass Carp reovirus and its protectively immunity assessment for Grass Carp
Aquaculture, 2020Co-Authors: De-kui Qiu, Zhao Zhao, Zi-rao Guo, Yi-jun Jia, Chen Zhang, Gao-xue Wang, Bin ZhuAbstract:Abstract Hemorrhagic disease caused by Grass Carp reovirus (GCRV) is considered to be one of the most serious threats to Grass Carp, resulting in significant economic loss in aquaculture industry. Currently, vaccination is the most effective method to against GCRV infection and improve the production of Grass Carp. In the study, the antigen epitope of GCRV-II was screened, and its protective effects on Grass Carp against GCRV-II infection using carbon nanotubes as a carrier were assessment. The vp4 gene of GCRV-II was divided into four segments and respectively expressed in Escherichia coli. The potential antigen epitope was screened by the enzyme linked immunosorbent assay (ELISA). Afterwards, the VP4 and VP4-3 were conjugated with single-walled carbon nanotubes (SWCNTs) to vaccinate Grass Carp, and the protective effects were assessed by checking immune related parameters (specific serum antibodies, enzyme activities and immune related genes). The results indicate that better immune responses of Grass Carp immunized with the SWCNTs-VP4 subunit vaccine were induced in comparison with naked VP4 subunit vaccine. Besides, compared to the SWCNTs-VP4 groups, the immune related parameters showed higher levels in SWCNTs-VP4-3 treatment groups. The survival of Grass Carp treated with SWCNTs-VP4-3 was 76.7% during 10 days post infection, while that were 58.3% and 0% for the SWCNTs-VP4 and control groups, respectively. These results indicated that SWCNTs-VP4-3 can be a plausible candidate for preventing and controlling GCRV-II among Grass Carp.
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The protective immunity against Grass Carp reovirus in Grass Carp induced by a DNA vaccination using single-walled carbon nanotubes as delivery vehicles.
Fish & shellfish immunology, 2015Co-Authors: Yuan Wang, Guang-lu Liu, Bin Zhu, Fei Ling, Gao-xue WangAbstract:To reduce the lethal hemorrhagic disease caused by Grass Carp reovirus (GCRV) and improve the production of Grass Carp, efficient and economic prophylactic measure against GCRV is the most pressing desired for the Grass Carp farming industry. In this work, a novel SWCNTs-pEGFP-vp5 DNA vaccine linked vp5 recombinant in the form of plasmid pEGFP-vp5 and ammonium-functionalized SWCNTs by a chemical modification method was prepared to enhance the efficacy of a vp5 DNA vaccine against GCRV in juvenile Grass Carp. After intramuscular injection (1, 2.5 and 5 μg) and bath administration (1, 10, and 20 mg/L), the ability of the different immune treatments to induce transgene expression was analyzed. The results showed that higher levels of transcription and expression of vp5 gene could be detected in muscle tissues of Grass Carp in SWCNTs-pEGFP-vp5 treatment groups compare with naked pEGFP-vp5 treatment groups. Moreover, antibody levels, immune-related genes, and relative percentage survival were significantly enhanced in fish immunized with SWCNTs-pEGFP-vp5 vaccine. In addition, we found that a good immune protective effect was observed in bath immunization group; which at a concentration of 20 mg/L could reach the similar relative percentage survival (approximately 100%) in injection group at a dose of 5 μg. All these results indicated that ammonium-functionalized SWCNTs could provide extensive application prospect to aquatic vaccine and might be used to vaccinate fish by intramuscular injection or bath administration method.
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Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of Grass Carp against Grass Carp reovirus
Fish & shellfish immunology, 2014Co-Authors: Bin Zhu, Guang-lu Liu, Yu-xin Gong, Fei Ling, Linsheng Song, Gao-xue WangAbstract:Grass Carp reovirus (GCRV), the most pathogenic aquareovirus, can cause fatal hemorrhagic disease in fingerling and yearling Grass Carp. Vaccination by injection is by far the most effective method of combating disease. However it is labor intensive, costly and not feasible to vaccinate large numbers of the fish. Thus, an efficient and economic strategy for the prevention of GCRV infection becomes urgent. Here, functionalized single-walled carbon nanotubes (SWCNTs) as carrier were used to manufacture SWCNTs-VP7 subunit vaccine with chemical modification. Different developmental stages of Grass Carps were immunized by VP7/SWCNTs-VP7 subunit vaccine against GCRV by intramuscular injection and bath immunization. The results indicate that better immune responses of Grass Carp immunized with the SWCNTs-VP7 subunit vaccine were induced in comparison with VP7 subunit vaccine alone. Immunization doses/concentrations are significantly reduced (about 5-8 times) to prevent GCRV infection in different developmental stages of Grass Carp with injection or bath treatment when SWCNTs carrier was used. A good immune protective effect (relative percentage survival greater than 95%) is observed in smaller size fish (0.2 g) with SWCNTs-VP7 bath immunization. In addition, serum respiratory burst activity, complement activity, lysozyme activity, superoxide dismutase activity, alkaline phosphatase activity, immune-related genes and antibody levels were significantly enhanced in fish immunized with vaccine. This study suggested that functionalized SWCNTs was the promising carrier for recombinant subunit vaccine and might be used to vaccinate fish by bath approach.
Yingying Wang - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a cell line from swim bladder of the Grass Carp (Ctenopharyngodon idellus) for propagation of Grass Carp Reovirus Genotype II.
Microbial pathogenesis, 2021Co-Authors: Yuru Yang, Weiwei Zeng, Qing Wang, Yingying Wang, Jiyuan Yin, Cunbin ShiAbstract:A cell line was established from swim bladder of the Grass Carp (Ctenopharyngodon idellus) (CiSB), which was permissive for infection and propagation of Grass Carp Reovirus (GCRV). CiSB cells displayed optimal growth at 27 °C using M199 medium containing 10% fetal bovine serum and a fibroblastic-like morphology. Karyotype analysis revealed that the average diploid chromosome number was 52 in 58% of cells at passage 60 compared to the wild type Grass Carp cells (2n = 48). Infection with GCRV II isolate Hunan1307 was tracked by immunofluorescence and virus titration assay. The virus titer reached 105.2 TCID50/mL on 7th days post infection (dpi). Healthy adult Grass Carp that were challenged with the virus propagated onto CiSB cells, displayed the typical symptoms and histopathological changes of Grass Carp hemorrhagic disease (GCHD). Therefore, the CiSB cells can be used to propagate GCRV II and serve as a useful tool to study the pathogenesis of GCHD.
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An iTRAQ-based comparative proteomic analysis of Grass Carp infected with virulent and avirulent isolates of Grass Carp reovirus genotype II
Aquaculture, 2021Co-Authors: Zhishen Huang, Yingying Wang, Jiyuan Yin, Sven M. Bergmann, Caixia Gao, Wenli Zhou, Ting Gao, Yahui WangAbstract:Abstract Genotype II of Grass Carp reovirus (GCRV) is an epidemic strain that is devastating to the Grass Carp industry in China. Infections of Grass Carp caused with virulent GCRV-HuNan1307 and avirulent GCRV-GD1108 isolates were carried out to determine the underlying pathogenic mechanisms. The differential host protein response was examined after infection with both virus isolates using an iTRAQ-based comparative quantitative proteomics. Differentially expressed proteins for GD1108 (34) and HuNan1307 (222) infections were identified and determined canonical pathways and functional networks involved in both GCRV infections. Infection with HuNan1307 activated the RIG-I pathway leading to antiviral response as well as the Jak-STAT pathway that governs general metabolic functions. These pathways were not activated by infection with the avirulent isolate. Infection with HuNan1307 also induced an abbreviated an intense immune response and lead to major disorders of the metabolism. In contrast, the GD1108 infection induced a mild and moderate immune response leaving basic metabolism unchanged. This study provides a comprehensive view at the protein level of the underlying pathogenic mechanisms used by a virulent GCRV type II isolate. Significance Genotype II of Grass Carp reovirus (GCRV) is an epidemic strain that is devastating to the Grass Carp industry in China. The unclear etiological characters, genetic variation and pathogenesis of GCRV genotype II blocked the development of techniques as well as products on prevention and control of Grass Carp haemorrhagic disease (GCHD). Here, iTRAQ strategy was firstly used to compare the proteomes of Grass Carp infected with virulent and avirulent isolates of GCRV genotype II. The obtained data showed that abbreviated and intense immune response and disorders of the metabolism were reasons for the mortality of fish infected with virulent GCRV isolate. The results would provide a train of thought to promote the development of techniques as well as products on prevention and control of GCHD.
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Comparison of the blood parameters and histopathology between Grass Carp infected with a virulent and avirulent isolates of genotype II Grass Carp reovirus.
Microbial pathogenesis, 2019Co-Authors: Tang Yafang, Weiwei Zeng, Qing Wang, Yingying Wang, Jiyuan Yin, Chengbao Wang, Sven M. Bergmann, Caixia GaoAbstract:Grass Carp hemorrhagic disease caused by Grass Carp reovirus (GCRV) is the most important disease for Grass Carp aquaculture. Its typical clinical symptom is haemorrhaging, although the mechanism was remained unclear. In this study, we investigated the differences in blood parameters and histopathological features between Grass Carp infected with a virulent and avirulent isolates of genotype II GCRV. Infection with the virulent isolate resulted in increases in 8 routine blood and 2 serum biochemical parameters (P
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Oral delivery of Bacillus subtilis spores expressing Grass Carp reovirus VP4 protein produces protection against Grass Carp reovirus infection.
Fish & shellfish immunology, 2018Co-Authors: Hongye Jiang, Weiwei Zeng, Qing Wang, Qing Bian, Pengli Ren, Hengchang Sun, Zhipeng Lin, Zeli Tang, Xinyi Zhou, Yingying WangAbstract:Grass Carp (Ctenopharyngodon idellus) hemorrhagic disease (GCHD), caused by Grass Carp reovirus (GCRV), has given rise to an enormous loss in Grass Carp industry during the past years. Up to date, vaccination remained to be the most effective way to protect Grass Carp from GCHD. Oral vaccination is of major interest due to its advantages of noninvasive, time-saving, and easily-operated. The introduction of oral vaccination has profound impact on aquaculture industry because of its feasibility of extensive application for fish in various size and age. However, the main challenge in developing oral vaccine is that antigens are easily degraded and are easy to induce tolerance. Bacillus subtilis (B. subtilis) spores would be an ideal oral vaccine delivery system for their robust specialty, gene operability, safety and adjuvant property. VP4 protein is the major outer capsid protein encoded by GCRV segment 6 (S6), which plays an important role in viral invasion and replication. In this study, we used B. subtilis spores as the oral delivery system and successfully constructed the B. subtilis CotC-VP4 recombinant spores (CotC-VP4 spores) to evaluate its protective efficacy in Grass Carp. Grass Carp orally immunized with CotC-VP4 spores showed a survival rate of 57% and the relative percent survival (RPS) of 47% after the viral challenge. Further, the specific IgM levels in serum and the specific IgZ levels in intestinal mucus were significantly higher in the CotC-VP4 group than those in the Naive group. The immune-related genes including three innate immune-related genes (IL-4/13A, IL-4/13B, CSF1R), four adaptive immune-related genes (BAFF, CD4L, MHC-II, CD8), three inflammation-related genes (IL-1β, TNF-α, TGF-β) and interferon type I (IFN-I) related signaling pathway genes were significantly up-regulated in the CotC-VP4 group. The study demonstrated that the CotC-VP4 spores produced protection in Grass Carp against GCRV infection, and triggered both innate and adaptive immunity post oral immunization. This work highlighted that Bacillus subtilis spores were powerful platforms for oral vaccine delivery, and the combination of Bacillus subtilis spores with GCRV VP4 protein was a promising oral vaccine.
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Immunogenicity of a cell culture-derived inactivated vaccine against a common virulent isolate of Grass Carp reovirus
Fish & shellfish immunology, 2016Co-Authors: Weiwei Zeng, Qing Wang, Yingying Wang, Changchen Zhao, Chunbin Shi, Song Xinjian, Huang QiwenAbstract:Grass Carp (Ctenopharyngodon idella) hemorrhagic disease, caused by Grass Carp reovirus (GCRV), is emerging as a serious problem in Grass Carp aquaculture. There is no available antiviral therapy and vaccination is the primary method of disease control. In the present study, the immunological effects and protective efficacy of an inactivated HuNan1307 vaccine in Grass Carp were evaluated. The GCRV isolate HuNan1307 was produced by replication onto the Grass Carp PSF cell line, and inactivated with 1% β-propiolactone for 60 h at 4 °C. Grass Carp were injected with inactivated GCRV vaccine, followed by challenge with the isolate HuNan1307. The results showed that the minimum dosage of the inactivated vaccine was 10(5.5) TCID50/0.2 mL to induce immune protection. All Grass Carp immunized with the inactivated vaccine produced a high titer of serum antibodies and GCRV-specific neutralizing antibody. Moreover, the inactivated vaccine injection increased the expression of 6 immune-related genes in the spleen and head kidney, which indicated that a immune response was induced by the HuNan1307 vaccine. In addition, Grass Carp immunized with the inactivated vaccine showed a survival rate above 80% after the viral challenge, equal to that of Grass Carp immunized with a commercial attenuated vaccine, and the protection lasted at least for one year. The data in this study suggested that the inactivated HuNan1307 vaccine may represent an efficient method to induce immunity against GCRV infection and the induced disease in Grass Carp.
Bin Zhu - One of the best experts on this subject based on the ideXlab platform.
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Antigen epitope screening of Grass Carp reovirus and its protectively immunity assessment for Grass Carp
Aquaculture, 2020Co-Authors: De-kui Qiu, Zhao Zhao, Zi-rao Guo, Yi-jun Jia, Chen Zhang, Gao-xue Wang, Bin ZhuAbstract:Abstract Hemorrhagic disease caused by Grass Carp reovirus (GCRV) is considered to be one of the most serious threats to Grass Carp, resulting in significant economic loss in aquaculture industry. Currently, vaccination is the most effective method to against GCRV infection and improve the production of Grass Carp. In the study, the antigen epitope of GCRV-II was screened, and its protective effects on Grass Carp against GCRV-II infection using carbon nanotubes as a carrier were assessment. The vp4 gene of GCRV-II was divided into four segments and respectively expressed in Escherichia coli. The potential antigen epitope was screened by the enzyme linked immunosorbent assay (ELISA). Afterwards, the VP4 and VP4-3 were conjugated with single-walled carbon nanotubes (SWCNTs) to vaccinate Grass Carp, and the protective effects were assessed by checking immune related parameters (specific serum antibodies, enzyme activities and immune related genes). The results indicate that better immune responses of Grass Carp immunized with the SWCNTs-VP4 subunit vaccine were induced in comparison with naked VP4 subunit vaccine. Besides, compared to the SWCNTs-VP4 groups, the immune related parameters showed higher levels in SWCNTs-VP4-3 treatment groups. The survival of Grass Carp treated with SWCNTs-VP4-3 was 76.7% during 10 days post infection, while that were 58.3% and 0% for the SWCNTs-VP4 and control groups, respectively. These results indicated that SWCNTs-VP4-3 can be a plausible candidate for preventing and controlling GCRV-II among Grass Carp.
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The protective immunity against Grass Carp reovirus in Grass Carp induced by a DNA vaccination using single-walled carbon nanotubes as delivery vehicles.
Fish & shellfish immunology, 2015Co-Authors: Yuan Wang, Guang-lu Liu, Bin Zhu, Fei Ling, Gao-xue WangAbstract:To reduce the lethal hemorrhagic disease caused by Grass Carp reovirus (GCRV) and improve the production of Grass Carp, efficient and economic prophylactic measure against GCRV is the most pressing desired for the Grass Carp farming industry. In this work, a novel SWCNTs-pEGFP-vp5 DNA vaccine linked vp5 recombinant in the form of plasmid pEGFP-vp5 and ammonium-functionalized SWCNTs by a chemical modification method was prepared to enhance the efficacy of a vp5 DNA vaccine against GCRV in juvenile Grass Carp. After intramuscular injection (1, 2.5 and 5 μg) and bath administration (1, 10, and 20 mg/L), the ability of the different immune treatments to induce transgene expression was analyzed. The results showed that higher levels of transcription and expression of vp5 gene could be detected in muscle tissues of Grass Carp in SWCNTs-pEGFP-vp5 treatment groups compare with naked pEGFP-vp5 treatment groups. Moreover, antibody levels, immune-related genes, and relative percentage survival were significantly enhanced in fish immunized with SWCNTs-pEGFP-vp5 vaccine. In addition, we found that a good immune protective effect was observed in bath immunization group; which at a concentration of 20 mg/L could reach the similar relative percentage survival (approximately 100%) in injection group at a dose of 5 μg. All these results indicated that ammonium-functionalized SWCNTs could provide extensive application prospect to aquatic vaccine and might be used to vaccinate fish by intramuscular injection or bath administration method.
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Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of Grass Carp against Grass Carp reovirus
Fish & shellfish immunology, 2014Co-Authors: Bin Zhu, Guang-lu Liu, Yu-xin Gong, Fei Ling, Linsheng Song, Gao-xue WangAbstract:Grass Carp reovirus (GCRV), the most pathogenic aquareovirus, can cause fatal hemorrhagic disease in fingerling and yearling Grass Carp. Vaccination by injection is by far the most effective method of combating disease. However it is labor intensive, costly and not feasible to vaccinate large numbers of the fish. Thus, an efficient and economic strategy for the prevention of GCRV infection becomes urgent. Here, functionalized single-walled carbon nanotubes (SWCNTs) as carrier were used to manufacture SWCNTs-VP7 subunit vaccine with chemical modification. Different developmental stages of Grass Carps were immunized by VP7/SWCNTs-VP7 subunit vaccine against GCRV by intramuscular injection and bath immunization. The results indicate that better immune responses of Grass Carp immunized with the SWCNTs-VP7 subunit vaccine were induced in comparison with VP7 subunit vaccine alone. Immunization doses/concentrations are significantly reduced (about 5-8 times) to prevent GCRV infection in different developmental stages of Grass Carp with injection or bath treatment when SWCNTs carrier was used. A good immune protective effect (relative percentage survival greater than 95%) is observed in smaller size fish (0.2 g) with SWCNTs-VP7 bath immunization. In addition, serum respiratory burst activity, complement activity, lysozyme activity, superoxide dismutase activity, alkaline phosphatase activity, immune-related genes and antibody levels were significantly enhanced in fish immunized with vaccine. This study suggested that functionalized SWCNTs was the promising carrier for recombinant subunit vaccine and might be used to vaccinate fish by bath approach.
Qing Wang - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a cell line from swim bladder of the Grass Carp (Ctenopharyngodon idellus) for propagation of Grass Carp Reovirus Genotype II.
Microbial pathogenesis, 2021Co-Authors: Yuru Yang, Weiwei Zeng, Qing Wang, Yingying Wang, Jiyuan Yin, Cunbin ShiAbstract:A cell line was established from swim bladder of the Grass Carp (Ctenopharyngodon idellus) (CiSB), which was permissive for infection and propagation of Grass Carp Reovirus (GCRV). CiSB cells displayed optimal growth at 27 °C using M199 medium containing 10% fetal bovine serum and a fibroblastic-like morphology. Karyotype analysis revealed that the average diploid chromosome number was 52 in 58% of cells at passage 60 compared to the wild type Grass Carp cells (2n = 48). Infection with GCRV II isolate Hunan1307 was tracked by immunofluorescence and virus titration assay. The virus titer reached 105.2 TCID50/mL on 7th days post infection (dpi). Healthy adult Grass Carp that were challenged with the virus propagated onto CiSB cells, displayed the typical symptoms and histopathological changes of Grass Carp hemorrhagic disease (GCHD). Therefore, the CiSB cells can be used to propagate GCRV II and serve as a useful tool to study the pathogenesis of GCHD.
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Comparison of the blood parameters and histopathology between Grass Carp infected with a virulent and avirulent isolates of genotype II Grass Carp reovirus.
Microbial pathogenesis, 2019Co-Authors: Tang Yafang, Weiwei Zeng, Qing Wang, Yingying Wang, Jiyuan Yin, Chengbao Wang, Sven M. Bergmann, Caixia GaoAbstract:Grass Carp hemorrhagic disease caused by Grass Carp reovirus (GCRV) is the most important disease for Grass Carp aquaculture. Its typical clinical symptom is haemorrhaging, although the mechanism was remained unclear. In this study, we investigated the differences in blood parameters and histopathological features between Grass Carp infected with a virulent and avirulent isolates of genotype II GCRV. Infection with the virulent isolate resulted in increases in 8 routine blood and 2 serum biochemical parameters (P
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Oral delivery of Bacillus subtilis spores expressing Grass Carp reovirus VP4 protein produces protection against Grass Carp reovirus infection.
Fish & shellfish immunology, 2018Co-Authors: Hongye Jiang, Weiwei Zeng, Qing Wang, Qing Bian, Pengli Ren, Hengchang Sun, Zhipeng Lin, Zeli Tang, Xinyi Zhou, Yingying WangAbstract:Grass Carp (Ctenopharyngodon idellus) hemorrhagic disease (GCHD), caused by Grass Carp reovirus (GCRV), has given rise to an enormous loss in Grass Carp industry during the past years. Up to date, vaccination remained to be the most effective way to protect Grass Carp from GCHD. Oral vaccination is of major interest due to its advantages of noninvasive, time-saving, and easily-operated. The introduction of oral vaccination has profound impact on aquaculture industry because of its feasibility of extensive application for fish in various size and age. However, the main challenge in developing oral vaccine is that antigens are easily degraded and are easy to induce tolerance. Bacillus subtilis (B. subtilis) spores would be an ideal oral vaccine delivery system for their robust specialty, gene operability, safety and adjuvant property. VP4 protein is the major outer capsid protein encoded by GCRV segment 6 (S6), which plays an important role in viral invasion and replication. In this study, we used B. subtilis spores as the oral delivery system and successfully constructed the B. subtilis CotC-VP4 recombinant spores (CotC-VP4 spores) to evaluate its protective efficacy in Grass Carp. Grass Carp orally immunized with CotC-VP4 spores showed a survival rate of 57% and the relative percent survival (RPS) of 47% after the viral challenge. Further, the specific IgM levels in serum and the specific IgZ levels in intestinal mucus were significantly higher in the CotC-VP4 group than those in the Naive group. The immune-related genes including three innate immune-related genes (IL-4/13A, IL-4/13B, CSF1R), four adaptive immune-related genes (BAFF, CD4L, MHC-II, CD8), three inflammation-related genes (IL-1β, TNF-α, TGF-β) and interferon type I (IFN-I) related signaling pathway genes were significantly up-regulated in the CotC-VP4 group. The study demonstrated that the CotC-VP4 spores produced protection in Grass Carp against GCRV infection, and triggered both innate and adaptive immunity post oral immunization. This work highlighted that Bacillus subtilis spores were powerful platforms for oral vaccine delivery, and the combination of Bacillus subtilis spores with GCRV VP4 protein was a promising oral vaccine.
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Immunogenicity of a cell culture-derived inactivated vaccine against a common virulent isolate of Grass Carp reovirus
Fish & shellfish immunology, 2016Co-Authors: Weiwei Zeng, Qing Wang, Yingying Wang, Changchen Zhao, Chunbin Shi, Song Xinjian, Huang QiwenAbstract:Grass Carp (Ctenopharyngodon idella) hemorrhagic disease, caused by Grass Carp reovirus (GCRV), is emerging as a serious problem in Grass Carp aquaculture. There is no available antiviral therapy and vaccination is the primary method of disease control. In the present study, the immunological effects and protective efficacy of an inactivated HuNan1307 vaccine in Grass Carp were evaluated. The GCRV isolate HuNan1307 was produced by replication onto the Grass Carp PSF cell line, and inactivated with 1% β-propiolactone for 60 h at 4 °C. Grass Carp were injected with inactivated GCRV vaccine, followed by challenge with the isolate HuNan1307. The results showed that the minimum dosage of the inactivated vaccine was 10(5.5) TCID50/0.2 mL to induce immune protection. All Grass Carp immunized with the inactivated vaccine produced a high titer of serum antibodies and GCRV-specific neutralizing antibody. Moreover, the inactivated vaccine injection increased the expression of 6 immune-related genes in the spleen and head kidney, which indicated that a immune response was induced by the HuNan1307 vaccine. In addition, Grass Carp immunized with the inactivated vaccine showed a survival rate above 80% after the viral challenge, equal to that of Grass Carp immunized with a commercial attenuated vaccine, and the protection lasted at least for one year. The data in this study suggested that the inactivated HuNan1307 vaccine may represent an efficient method to induce immunity against GCRV infection and the induced disease in Grass Carp.
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Pathogenicity and tissue distribution of Grass Carp reovirus after intraperitoneal administration
Virology journal, 2014Co-Authors: Liang Hongru, Weiwei Zeng, Yingying Wang, Qing WangAbstract:Grass Carp reovirus (GCRV) is the causative agent of Grass Carp hemorrhage and causes significant loss of fingerlings. However, little is known about how the virus is distributed in organs and tissues. The aim of the present study was to investigate the distribution of different GCRV stains in tissues and organs of Grass Carp. The pathogenicity and tissue distribution of GCRV were monitored after intraperitoneal administration. The study showed a distribution of GCRV in different tissues and organs, particularly in the liver, spleen, kidney, intestine, and muscle, which had a higher number of viral RNA copies during the sixth to ninth days. The kidney had the highest numbers of viral RNA copies, as high as 24000 copies. Until the fourteenth day, nearly no viral RNA copies could be detected. This study defined the virus distribution in different tissues of Grass Carp inoculated by i.p. and supplied clues for the pathogenesis of GCRV.
Weiwei Zeng - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a cell line from swim bladder of the Grass Carp (Ctenopharyngodon idellus) for propagation of Grass Carp Reovirus Genotype II.
Microbial pathogenesis, 2021Co-Authors: Yuru Yang, Weiwei Zeng, Qing Wang, Yingying Wang, Jiyuan Yin, Cunbin ShiAbstract:A cell line was established from swim bladder of the Grass Carp (Ctenopharyngodon idellus) (CiSB), which was permissive for infection and propagation of Grass Carp Reovirus (GCRV). CiSB cells displayed optimal growth at 27 °C using M199 medium containing 10% fetal bovine serum and a fibroblastic-like morphology. Karyotype analysis revealed that the average diploid chromosome number was 52 in 58% of cells at passage 60 compared to the wild type Grass Carp cells (2n = 48). Infection with GCRV II isolate Hunan1307 was tracked by immunofluorescence and virus titration assay. The virus titer reached 105.2 TCID50/mL on 7th days post infection (dpi). Healthy adult Grass Carp that were challenged with the virus propagated onto CiSB cells, displayed the typical symptoms and histopathological changes of Grass Carp hemorrhagic disease (GCHD). Therefore, the CiSB cells can be used to propagate GCRV II and serve as a useful tool to study the pathogenesis of GCHD.
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Comparison of the blood parameters and histopathology between Grass Carp infected with a virulent and avirulent isolates of genotype II Grass Carp reovirus.
Microbial pathogenesis, 2019Co-Authors: Tang Yafang, Weiwei Zeng, Qing Wang, Yingying Wang, Jiyuan Yin, Chengbao Wang, Sven M. Bergmann, Caixia GaoAbstract:Grass Carp hemorrhagic disease caused by Grass Carp reovirus (GCRV) is the most important disease for Grass Carp aquaculture. Its typical clinical symptom is haemorrhaging, although the mechanism was remained unclear. In this study, we investigated the differences in blood parameters and histopathological features between Grass Carp infected with a virulent and avirulent isolates of genotype II GCRV. Infection with the virulent isolate resulted in increases in 8 routine blood and 2 serum biochemical parameters (P
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Oral delivery of Bacillus subtilis spores expressing Grass Carp reovirus VP4 protein produces protection against Grass Carp reovirus infection.
Fish & shellfish immunology, 2018Co-Authors: Hongye Jiang, Weiwei Zeng, Qing Wang, Qing Bian, Pengli Ren, Hengchang Sun, Zhipeng Lin, Zeli Tang, Xinyi Zhou, Yingying WangAbstract:Grass Carp (Ctenopharyngodon idellus) hemorrhagic disease (GCHD), caused by Grass Carp reovirus (GCRV), has given rise to an enormous loss in Grass Carp industry during the past years. Up to date, vaccination remained to be the most effective way to protect Grass Carp from GCHD. Oral vaccination is of major interest due to its advantages of noninvasive, time-saving, and easily-operated. The introduction of oral vaccination has profound impact on aquaculture industry because of its feasibility of extensive application for fish in various size and age. However, the main challenge in developing oral vaccine is that antigens are easily degraded and are easy to induce tolerance. Bacillus subtilis (B. subtilis) spores would be an ideal oral vaccine delivery system for their robust specialty, gene operability, safety and adjuvant property. VP4 protein is the major outer capsid protein encoded by GCRV segment 6 (S6), which plays an important role in viral invasion and replication. In this study, we used B. subtilis spores as the oral delivery system and successfully constructed the B. subtilis CotC-VP4 recombinant spores (CotC-VP4 spores) to evaluate its protective efficacy in Grass Carp. Grass Carp orally immunized with CotC-VP4 spores showed a survival rate of 57% and the relative percent survival (RPS) of 47% after the viral challenge. Further, the specific IgM levels in serum and the specific IgZ levels in intestinal mucus were significantly higher in the CotC-VP4 group than those in the Naive group. The immune-related genes including three innate immune-related genes (IL-4/13A, IL-4/13B, CSF1R), four adaptive immune-related genes (BAFF, CD4L, MHC-II, CD8), three inflammation-related genes (IL-1β, TNF-α, TGF-β) and interferon type I (IFN-I) related signaling pathway genes were significantly up-regulated in the CotC-VP4 group. The study demonstrated that the CotC-VP4 spores produced protection in Grass Carp against GCRV infection, and triggered both innate and adaptive immunity post oral immunization. This work highlighted that Bacillus subtilis spores were powerful platforms for oral vaccine delivery, and the combination of Bacillus subtilis spores with GCRV VP4 protein was a promising oral vaccine.
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Immunogenicity of a cell culture-derived inactivated vaccine against a common virulent isolate of Grass Carp reovirus
Fish & shellfish immunology, 2016Co-Authors: Weiwei Zeng, Qing Wang, Yingying Wang, Changchen Zhao, Chunbin Shi, Song Xinjian, Huang QiwenAbstract:Grass Carp (Ctenopharyngodon idella) hemorrhagic disease, caused by Grass Carp reovirus (GCRV), is emerging as a serious problem in Grass Carp aquaculture. There is no available antiviral therapy and vaccination is the primary method of disease control. In the present study, the immunological effects and protective efficacy of an inactivated HuNan1307 vaccine in Grass Carp were evaluated. The GCRV isolate HuNan1307 was produced by replication onto the Grass Carp PSF cell line, and inactivated with 1% β-propiolactone for 60 h at 4 °C. Grass Carp were injected with inactivated GCRV vaccine, followed by challenge with the isolate HuNan1307. The results showed that the minimum dosage of the inactivated vaccine was 10(5.5) TCID50/0.2 mL to induce immune protection. All Grass Carp immunized with the inactivated vaccine produced a high titer of serum antibodies and GCRV-specific neutralizing antibody. Moreover, the inactivated vaccine injection increased the expression of 6 immune-related genes in the spleen and head kidney, which indicated that a immune response was induced by the HuNan1307 vaccine. In addition, Grass Carp immunized with the inactivated vaccine showed a survival rate above 80% after the viral challenge, equal to that of Grass Carp immunized with a commercial attenuated vaccine, and the protection lasted at least for one year. The data in this study suggested that the inactivated HuNan1307 vaccine may represent an efficient method to induce immunity against GCRV infection and the induced disease in Grass Carp.
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Pathogenicity and tissue distribution of Grass Carp reovirus after intraperitoneal administration
Virology journal, 2014Co-Authors: Liang Hongru, Weiwei Zeng, Yingying Wang, Qing WangAbstract:Grass Carp reovirus (GCRV) is the causative agent of Grass Carp hemorrhage and causes significant loss of fingerlings. However, little is known about how the virus is distributed in organs and tissues. The aim of the present study was to investigate the distribution of different GCRV stains in tissues and organs of Grass Carp. The pathogenicity and tissue distribution of GCRV were monitored after intraperitoneal administration. The study showed a distribution of GCRV in different tissues and organs, particularly in the liver, spleen, kidney, intestine, and muscle, which had a higher number of viral RNA copies during the sixth to ninth days. The kidney had the highest numbers of viral RNA copies, as high as 24000 copies. Until the fourteenth day, nearly no viral RNA copies could be detected. This study defined the virus distribution in different tissues of Grass Carp inoculated by i.p. and supplied clues for the pathogenesis of GCRV.
