The Experts below are selected from a list of 2469 Experts worldwide ranked by ideXlab platform
Guan Hong-quan - One of the best experts on this subject based on the ideXlab platform.
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Effects of Grifola frondosa Polysaccharide on T cell Subsets and Th1/Th2 Subsets of Spleen Deficiency Mice
Chinese Journal of Information on Traditional Chinese Medicine, 2013Co-Authors: Guan Hong-quanAbstract:Objective To observe the effects of Grifola frondosa polysaccharide on T cell subsets and Th1/Th2 subsets of spleen deficiency mice,and investigate its immunoregulation mechanism.Methods Forty-eight KM mice were divided into 6 groups:normal group,spleen deficiency group,positive group(lentinan),three dosage groups of Grifola frondosa polysaccharide,8 mice in each group.The mice were injected corresponding drug intraperitoneally for 10 days.Then mice in each group were detected CD3+,CD4+ and CD8+T cell by flow cytometry.IFN-γ and IL-4 levels were detected by ELISA.Results The high dose of Grifola frondosa polysaccharide could significantly increase the CD4+T lymphocytes percentage in peripheral blood(P 0.05).Medium and high dose of Grifola frondosa polysaccharide could significantly increase CD4+/CD8+T cell ratio(P 0.01) and CD3+T lymphocyte percentage(P 0.01) of spleen deficiency mice.Medium and high dose of Grifola frondosa polysaccharide could significantly increase IFN-γ level in serum of spleen deficiency mice(P 0.01),and high dose of Grifola frondosa polysaccharide could decrease IL-4 level in serum of spleen deficiency mice(P 0.01).Conclusion Grifola frondosa polysaccharide can improve the decreased CD4+/CD8+T cell ratio which caused by spleen deficiency,and promote the transformation of Th1 to Th2,thus treating spleen deficiency syndrome.
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Effects of Extract of Grifola frondosa on Bcl-2 and Bax in Spleen of Spleen Deficiency Mice
Chinese Journal of Experimental Traditional Medical Formulae, 2012Co-Authors: Guan Hong-quanAbstract:Objective:To investigate the effect of extract of Grifola frondosa on Bcl-2 and Bax in spleen of spleen deficiency mouse.Method:Forty-eight KM mice were divided into 6 groups:normal group,spleen deficiency group,lentinan group(2 mg·kg-1),three groups of extract of Grifola frondosa(5,10,20 mg·kg-1),ip ten days.Explore the expression of Bcl-2 and Bax in spleen of spleen deficiency mice by SP immunohistochemistry staining and RT-PCR.Result:The expression of Bcl-2 decreased,and the expression of Bax increased in spleen deficiency group.After given the extract of Grifola frondosa,the expression of Bcl-2 increased significantly(P0.01),and the expression of Bax decreased significantly(P0.01).Conclusion:The extract of Grifola frondosa could maintain the immunologic function by upregulating the expression of Bcl-2 and downregulating the expression of Bax in spleen of spleen deficiency mice.
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Effects of Extract of Grifola frondosa on Peritoneal Macrophages and Spleen Cells in Mouse with Spleen Deficiency
Chinese Journal of Experimental Traditional Medical Formulae, 2011Co-Authors: Guan Hong-quanAbstract:Objective:To investigate the immunological mechanism of extract of Grifola frondosa on spleen deficiency mice.Method:Thirty Kunming mice were randomly divided into 6 groups:control group,spleen deficiency group,positive control group and three groups with dosage difference(5,10 and 20 mg·kg-1).After the establishment of spleen deficiency,the treatment of the extract and the positive drug was lasted for 10 days(ip).Then phagocytosis of peritoneal macrophages was detected by neutral red;NO-producing ability was determined by Griess method;and lymphocyte transformation rate,NK cell activity were tested by MTT method.Result:Extract of Grifola frondosa could increase the above mentioned ability.Conclusion:Extract of Grifola frondosa can enhance the ability of macrophages and the production of NO,and the lymphocyte transformation rate,NK cell killing activity to improve immunological ability of spleen deficiency in mice.
Barrie R Cassileth - One of the best experts on this subject based on the ideXlab platform.
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a phase i ii trial of a polysaccharide extract from Grifola frondosa maitake mushroom in breast cancer patients immunological effects
Journal of Cancer Research and Clinical Oncology, 2009Co-Authors: Gary Deng, Hong Lin, Andrew D Seidman, Monica Fornier, Gabriella Dandrea, Kathleen Wesa, Simon Yeung, Susanna Cunninghamrundles, Andrew J Vickers, Barrie R CassilethAbstract:Background Cancer patients commonly use dietary supplements to “boost immune function”. A polysaccharide extract from Grifola frondosa (Maitake extract) showed immunomodulatory effects in preclinical studies and therefore the potential for clinical use. Whether oral administration in human produces measurable immunologic effects, however, is unknown.
Guoqiang Zhao - One of the best experts on this subject based on the ideXlab platform.
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isolation purification and antioxidant activities of polysaccharides from Grifola frondosa
Carbohydrate Polymers, 2012Co-Authors: Guitang Chen, Shengtao Liu, Yanli Liao, Guoqiang ZhaoAbstract:The crude polysaccharides (GFP) were isolated from the fruiting bodies of Grifola frondosa and purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in that order. Three main fractions, GFP-1, GFP-2 and GFP-3, were obtained through the isolation and purification steps. Then the antioxidant activities of these three fractions were investigated in vitro. The results showed that GFP-1, GFP-2 and GFP-3 possessed significant inhibitory effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical; their reducing power, ferrous ions chelating effect and the inhibition ability of the rat liver lipid oxidation where also strong. These results suggest that G. frondosa polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans.
Masahiko Ikeda - One of the best experts on this subject based on the ideXlab platform.
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antiatherosclerotic effect of the edible mushrooms pleurotus eryngii eringi Grifola frondosa maitake and hypsizygus marmoreus bunashimeji in apolipoprotein e deficient mice
Nutrition Research, 2008Co-Authors: Koichiro Mori, Chinatsu Kobayashi, Takako Tomita, Satoshi Inatomi, Masahiko IkedaAbstract:Abstract In the present study, we examined the antiatherosclerotic effects of 3 edible mushrooms, Pleurotus eryngii (Eringi), Grifola frondosa (Maitake), and Hypsizygus marmoreus (Bunashimeji), in atherosclerosis-susceptible C57BL/6J, apolipoprotein E–deficient (apoE −/− ) mice. Male apoE −/− mice (6 weeks of age) were fed a normal diet (cholesterol concentration
Gary Deng - One of the best experts on this subject based on the ideXlab platform.
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a phase i ii trial of a polysaccharide extract from Grifola frondosa maitake mushroom in breast cancer patients immunological effects
Journal of Cancer Research and Clinical Oncology, 2009Co-Authors: Gary Deng, Hong Lin, Andrew D Seidman, Monica Fornier, Gabriella Dandrea, Kathleen Wesa, Simon Yeung, Susanna Cunninghamrundles, Andrew J Vickers, Barrie R CassilethAbstract:Background Cancer patients commonly use dietary supplements to “boost immune function”. A polysaccharide extract from Grifola frondosa (Maitake extract) showed immunomodulatory effects in preclinical studies and therefore the potential for clinical use. Whether oral administration in human produces measurable immunologic effects, however, is unknown.
