The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ali Abas Wani - One of the best experts on this subject based on the ideXlab platform.
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rice bran nutritional values and its emerging potential for development of functional food a review
Bioactive Carbohydrates and Dietary Fibre, 2015Co-Authors: Khalid Gul, Basharat Yousuf, A K Singh, Preeti Singh, Ali Abas WaniAbstract:Abstract Rice bran is a by-product of rice milling industry and constitutes around 10% of the total weight of rough rice. It is primarily composed of aleurone, pericarp, subaleurone layer and germ. Rice bran is a rich source of vitamins, minerals, essential fatty acids, dietary fiber and other sterols. There is a widespread scientific agreement on various Health benefits associated with consumption of dietary fiber. Consumer attitude towards Health Foods is promising and the scope of functional Foods is growing in the world markets; rice bran is finding increased applications in food, nutraceutical and pharmaceutical industries. However, potential applications of rice bran in food industry are limited by its instability owing to rancidity caused by exposure of oil to lipases during milling. Various methods of stabilization have been carried out, paving way for supplementation of rice bran in numerous food preparations. Considering the importance of rice bran, this review aims to focus on the functionalities of rice bran, its Health benefits and potential applications in food industry.
Quanfu Wang - One of the best experts on this subject based on the ideXlab platform.
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cold adapted glutathione s transferases from antarctic psychrophilic bacterium halomonas sp ant108 heterologous expression characterization and oxidative resistance
Marine Drugs, 2019Co-Authors: Yanhua Hou, Chenhui Qiao, Yifan Wang, Yatong Wang, Xiulian Ren, Qifeng Wei, Quanfu WangAbstract:Glutathione S-transferases are one of the most important antioxidant enzymes to protect against oxidative damage induced by reactive oxygen species. In this study, a novel gst gene, designated as hsgst, was derived from Antarctic sea ice bacterium Halomonas sp. ANT108 and expressed in Escherichia coli (E. coli) BL21. The hsgst gene was 603 bp in length and encoded a protein of 200 amino acids. Compared with the mesophilic EcGST, homology modeling indicated HsGST had some structural characteristics of cold-adapted enzymes, such as higher frequency of glycine residues, lower frequency of proline and arginine residues, and reduced electrostatic interactions, which might be in relation to the high catalytic efficiency at low temperature. The recombinant HsGST (rHsGST) was purified to apparent homogeneity with Ni-affinity chromatography and its biochemical properties were investigated. The specific activity of the purified rHsGST was 254.20 nmol/min/mg. The optimum temperature and pH of enzyme were 25 °C and 7.5, respectively. Most importantly, rHsGST retained 41.67% of its maximal activity at 0 °C. 2.0 M NaCl and 0.2% H₂O₂ had no effect on the enzyme activity. Moreover, rHsGST exhibited its protective effects against oxidative stresses in E. coli cells. Due to its high catalytic efficiency and oxidative resistance at low temperature, rHsGST may be a potential candidate as antioxidant in low temperature Health Foods.
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Cold-Adapted Glutathione S-Transferases from Antarctic Psychrophilic Bacterium Halomonas sp. ANT108: Heterologous Expression, Characterization, and Oxidative Resistance
MDPI AG, 2019Co-Authors: Yanhua Hou, Chenhui Qiao, Yifan Wang, Yatong Wang, Xiulian Ren, Qifeng Wei, Quanfu WangAbstract:Glutathione S-transferases are one of the most important antioxidant enzymes to protect against oxidative damage induced by reactive oxygen species. In this study, a novel gst gene, designated as hsgst, was derived from Antarctic sea ice bacterium Halomonas sp. ANT108 and expressed in Escherichia coli (E. coli) BL21. The hsgst gene was 603 bp in length and encoded a protein of 200 amino acids. Compared with the mesophilic EcGST, homology modeling indicated HsGST had some structural characteristics of cold-adapted enzymes, such as higher frequency of glycine residues, lower frequency of proline and arginine residues, and reduced electrostatic interactions, which might be in relation to the high catalytic efficiency at low temperature. The recombinant HsGST (rHsGST) was purified to apparent homogeneity with Ni-affinity chromatography and its biochemical properties were investigated. The specific activity of the purified rHsGST was 254.20 nmol/min/mg. The optimum temperature and pH of enzyme were 25 °C and 7.5, respectively. Most importantly, rHsGST retained 41.67% of its maximal activity at 0 °C. 2.0 M NaCl and 0.2% H2O2 had no effect on the enzyme activity. Moreover, rHsGST exhibited its protective effects against oxidative stresses in E. coli cells. Due to its high catalytic efficiency and oxidative resistance at low temperature, rHsGST may be a potential candidate as antioxidant in low temperature Health Foods
Yoshiteru Oshima - One of the best experts on this subject based on the ideXlab platform.
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a novel carbon skeletal trichothecane tenuipesine a isolated from an entomopathogenic fungus paecilomyces tenuipes
Organic Letters, 2004Co-Authors: Haruhisa Kikuchi, Yasuhiro Miyagawa, Yuko Sahashi, Satoshi Inatomi, Koji Nakamura, Yoshiteru OshimaAbstract:Tenuipesine A (1), a novel trichothecane with an unprecedented carbon-migrated skeleton that embodies of a cyclopropane ring, was isolated from cultivated fruiting bodies of Paecilomyces tenuipes (Isaria japonica), a popular entomopathogenic fungi employed in folk medicine and Health Foods in China, Korea, and Japan. The structure was determined on the basis of two-dimensional NMR data. Its stereochemistry was elucidated by spectroscopic data and the chemical transformation of the coexisting trichothecene, 4beta-acetoxy-12,13-epoxytrichothec-9-ene-3alpha,15-diol (2). [structure: see text]
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Novel trichothecanes, paecilomycine A, B, and C, isolated from entomopathogenic fungus, Paecilomyces tenuipes
Tetrahedron Letters, 2004Co-Authors: Haruhisa Kikuchi, Yasuhiro Miyagawa, Yuko Sahashi, Satoshi Inatomi, Asami Haganuma, Norimichi Nakahata, Yoshiteru OshimaAbstract:Paecilomycine A (1), B (2), and C (3) were isolated from cultivated fruiting bodies of Paecilomyces tenuipes (Isaria japonica), which is a popular entomopathogenic fungus used in folk medicine and Health Foods in China, Korea, and Japan. The structures of 1–3 were deduced from their spectroscopic data and their absolute configurations were elucidated by preparing their MPA esters. Compound 1 showed activity in the neurotrophic factor biosynthesis in glial cells.
Beata Olas - One of the best experts on this subject based on the ideXlab platform.
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the beneficial Health aspects of sea buckthorn elaeagnus rhamnoides l a nelson oil
Journal of Ethnopharmacology, 2018Co-Authors: Beata OlasAbstract:Abstract Ethnopharmacological context Plant oils are known to have biological activity. This review paper summarizes the current knowledge of the composition of sea buckthorn (Elaeagnus rhamnoides (L.) A.Nelson) seed and pulp oil and its beneficial Health aspects. Materials and methods In vitro and in vivo studies on humans and animals have found sea buckthorn oil to have a variety of beneficial properties to human Health, and indicate that it may be a valuable component of human and animal nutrition. Various bioactive substances are present in all parts of sea buckthorn, and these are used traditionally as raw material for Health Foods and as nutritional supplements. The oil, berries, leaves and bark have medicinal properties, and the fruits have a unique taste; these parts can be processed to make oil, juice, jam, jellies and candies, as well as alcoholic and non-alcoholic beverages. Results Sea buckthorn oil may be extracted from the seed or the pulp. The mature seeds contain 8–20% oil and the dried fruit pulp about 20–25%, while the fruit residue contains about 15–20% oil after juice extraction. These oils have high concentrations of lipophilic constituents, most commonly unsaturated fatty acids (UFAs), phytosterols and vitamins A and E. These components have a multifunctional effect on human Health, with the fatty acids playing an important function in modifying cerebrovascular and cardiovascular disorders. The oil also has anti-oxidant, anti-inflammatory and anti-depressive properties. Conclusion Sea buckthorn is a unique plant. Its beneficial properties against cardiovascular disorders have been attributed to its high UFA content and range of phytosterols, especially beta-sitosterol. However, its different action on the human organism remain unclear, and further well-controlled, high-quality experiments with human subjects are required to determine the prophylactic and therapeutic doses of sea buckthorn oil for use in clinical studies. Additional studies are also needed to understand the action by which the oil exerts its beneficial properties, i.e. its cardioprotective and anti-cancer activity.
Yukihiro Goda - One of the best experts on this subject based on the ideXlab platform.
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disintegration test of Health food products containing ginkgo biloba l or vitex agnus castus l in the japanese market
Medicines, 2015Co-Authors: Naoko Satomasumoto, Takashi Hakamatsuka, Sayaka Masada, Satoshi Takahashi, Sachiko Terasaki, Yoichi Yokota, Yukihiro GodaAbstract:For many years now, a number of Western herbs have been widely used in Health food products in Japan and as pharmaceuticals in Europe. There are few or no mandated criteria concerning the quality of these herbal Health food products, thus clarification is warranted. Here, we performed disintegration tests of 26 pharmaceutical and Health food products containing the Western herbs ginkgo leaf and chaste tree fruit, in accord with the Japanese Pharmacopoeia. All eight pharmaceutical herbal products found in the European market completely disintegrated within the defined test time, and 11 of the 18 tested herbal products distributed as Health Foods in Japan disintegrated. Among the incompatible products identified in the Pharmacopoeia test, some products remained intact after incubation in water for 60 min. To ensure the efficacy of Western herbal products sold as Health food in Japan, quality control, including disintegration, is therefore recommended, even though these products are not regulated under the Pharmaceutical Affairs Law.
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quality evaluation of medicinal products and Health Foods containing chaste berry vitex agnus castus in japanese european and american markets
Chemical & Pharmaceutical Bulletin, 2014Co-Authors: Masahiro Fukahori, Shojiro Kobayashi, Yoko Naraki, Takahiro Sasaki, Hideki Oka, Masaharu Seki, Sayaka Masadaatsumi, Takashi Hakamatsuka, Yukihiro GodaAbstract:The aim of present study was to evaluate the qualities of chaste berry (fruit of Vitex agnus-castus L.) preparations using HPLC fingerprint analysis. Seven medicinal products 1 from Japan and 6 from Europe, and 17 Health Foods, 6 from Japan and 11 from the United States were analyzed. HPLC profile and 26 authentic peaks were compared medicinal products and Health Foods. Whereas medicinal products had similar HPLC profiles, Health Foods had various profiles and each peak was also greatly different. The measured amounts of two markers in 5 traditional medicinal products, agnuside and casticin specified in the European Pharmacopoeia (EP), the U.S. Pharmacopoeia (USP) or the WHO monographs of chaste berry, were much lower than those in 2 medicinal products defined as "well-established use" by the European Medicines Agency. The amounts of two markers for 17 Health Foods differed in a great deal from 14-5054% and 3-1272%, respectively. Furthermore the amount ratios of two markers, agnuside/casticin, in about half of the Health Foods were remarkably larger than the standard crude drug and the ratios were closer to one of the related Chinese herbs, Vitex negundo L. It is concluded that a combination of HPLC fingerprints and the amount ratios of the marker compounds of chaste berry preparations serves as a useful tool to evaluate the qualities of these preparations.
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genetic and chemical diversity of eleutherococcus senticosus and molecular identification of siberian ginseng by pcr rflp analysis based on chloroplast trnk intron sequence
Food Chemistry, 2011Co-Authors: Shu Zhu, Takuro Maruyama, Takeshi Kawasaki, Masao Fujita, Yanjing Bai, Mayuko Oya, Ken Tanaka, Katsuko Komatsu, Yukihiro Goda, Toshiro ShibataAbstract:Abstract Siberian ginseng (SG), the rhizome and root of Eleutherococcus senticosus , has been used as a tonic and anti-fatigue agent in northeastern Asia from ancient time. In recent years, SG has been becoming fairly popular as dietary supplements and Health Foods worldwide. In order to establish a convenient and sensitive method for authentication, chloroplast trn K intron sequences of 6 Eleutherococcus species were determined and compared. Genetic polymorphism, representing by 14 types of trn K intron sequence, in E. senticosus was observed. However, characteristic nucleotide markers stable within this species enabled clear discrimination of it from other congeners. A PCR-RFLP method was further developed, which was demonstrated to be efficient for authentication of crude drugs as well as Health Foods. Quantitative evaluation of three main bioactive constituents indicated chemical diversity in E. senticosus collected from northeast China and the results suggested good producing areas of SG. The chemical data clearly revealed that E. sessliflorus was unsuitable to be used as SG.
