The Experts below are selected from a list of 84 Experts worldwide ranked by ideXlab platform
Teruo Kumazawa - One of the best experts on this subject based on the ideXlab platform.
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effects of cibenzoline on cardiac function and metabolism in the rat Heart Lung Preparation
European Journal of Anaesthesiology, 2001Co-Authors: Atsushi Furuya, M Kume, S Kashimoto, Teruo KumazawaAbstract:Summary Background and objective Although there is concern that cibenzoline, an antidysrhythmic drug for the treatment of ventricular and supraventricular dysrhythmias, may be associated with dose-dependent inhibition of myocardial contractility there are few reports about the relationship between myocardial metabolism and cardiac function when it is used. The present study was designed to investigate the effects of cibenzoline on cardiac function and metabolism. The effects of cibenzoline on cardiac function and myocardial metabolism were assessed in the isolated rat Heart–Lung Preparation. Methods Thirty-two male Wistar-ST rats were divided into four groups: control, and those to receive cibenzoline, either 300, 900 or 3000 ng mL−1. The cibenzoline was administered into the perfusate 5 min after the start of perfusion. Heart rates in the 3000 ng mL−1 group were significantly lower than those in the control group. Cardiac output in the 3000 ng mL−1 group at 15 and 30 min was significantly lower than in the control group. In all groups, values for %LV dP/dt max (the ratio of values at each time to those at 5 min) at 20, 25, 30 min were significantly higher than at 5 min. Myocardial adenosine triphosphate concentration in the 3000 ng mL−1 group was significantly lower than in controls. There was no difference between groups in the lactate/pyruvate ratio. Conclusion The therapeutic range of cibenzoline has few effects on cardiac function and metabolism, although concentrations 10 times greater may cause a deterioration in myocardial metabolism.
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amrinone improves postischemic myocardial metabolism in the rat Heart Lung Preparation
Journal of Anesthesia, 1996Co-Authors: M Kume, Satoshi Kashimoto, Atsusi Furuya, Teruo KumazawaAbstract:Amrinone, a phosphodiesterase inhibitor, is a non-glycosidic noncatecholamine with both vasodilator and positive inotropic effects. We were interested in assessing the effect of amrinone on postischemic cardiac performance in the isolated Heart-Lung Preparation. Twenty-four male Wistar-ST rats were used. They were randomly divided into three groups. Amrinone, 10 μg·ml−1 or 100 μg·ml−1 was administered 8 min after the start of perfusion except in the control group. Ten minutes after the start of perfusion, all Hearts were made globally ischemic for 8 min. Subsequently, the Preparations were reperfused for 10 min. At the end of the experimental period, the Hearts were freeze-clamped, and then myocardial high-energy phosphates, lactate, pyruvate, and glycogen were measured. Hemodynamic parameters in all groups decreased significantly during ischemia. However, there were no significant differences among the groups. The myocardial ATP level in the 100 μg·ml−1 group was significantly higher than that in the control group. Adenosine diphosphate (ADP) and adenosine monophosphate (AMP) levels in the 100 μg·ml−1 group were significantly lower than those in the control group. Myocardial lactate, pyruvate, and glycogen levels were not significantly different among the groups. This result suggests that amrinone improves postischemic myocardial metabolism. Although we could not measure coronary flow, amrinone might increase coronary flow with direct coronary vasodilation which would have increased the myocardial ATP and energy charge levels.
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flumazenil does not antagonize the cardiac effects of midazolam in the isolated rat Heart Lung Preparation
Journal of Anesthesia, 1996Co-Authors: Toshihiro Nakamura, Satoshi Kashimoto, Akihiko Nonaka, Teruo KumazawaAbstract:We examined the effects of midazolam and flumazenil on cardiac function and metabolism in the isolated rat Heart-Lung Preparation. Wistar rats were divided into five groups (each group:n=8) as follows: (1) control (saline); (2) flumazenil (1.3×10−5M); (3) flumazenil (10−4M); (4) midazolam (60μg·ml−1); and (5) midazolam (60μg·ml−1) and flumazenil (1.3×10−5M). Systolic blood pressure and calculated left ventricular dP/dt maximum in the midazolam or midazolam conbined with flumazenil groups increased significantly in comparison with those in the control group. Heart rate in the midazolam group was lower than that in the control group. However, in the flumazenil group, there were no effects on the hemodynamics. There were no significant differences in the myocardial tissue concentration of ATP, lactate, and glycogen in all groups. In this study, midazolam decreased Heart rate; however, flumazenil had no effect on the Heart, nor did it antagonize the cardiac effects of midazolam. These results suggest that flumazenil has no effect on the peripheraltype benzodiazepine receptor of the myocardium.
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cardiac effects of methylmethacrylate in the rat Heart Lung Preparation with or without volatile anesthetics
Resuscitation, 1995Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, Akihiko Nonaka, Atsushi Furuya, Takashi Matsukawa, Teruo KumazawaAbstract:Abstract We have assessed the deleterious effects of methylmethacrylate (MMA) on cardiac function and metabolism in the isolated Heart-Lung Preparation with or without volatile anesthetics. Wistar rats were prepared for the Heart-Lung model. They were randomly divided into 5 groups as follows. (1) Control (C) group. (2) Cement (M) group; they received MMA. (3) Halothane (H) group; they received MMA and 1% halothane. (4) Isoflurane (I) group; they received MMA and 1.5% isoflurane. (5) Sevoflurane (S) group; they received MMA and 2.5% sevoflurane. MMA 1000 μg/ml was administered 7 min after the start of perfusion except in the C group. At the end of the experimental period, the Hearts were freeze-clamped and then myocardial high energy phosphates, lactate and glycogen were measured. Cardiac output in all groups but C group decreased significantly. PO 2 of the perfusion blood in the M, H, I and S groups was significantly lower than that in the C group. Myocardial ATP in the M, H, I and S groups was significantly lower than that in the C group. ADP and AMP in the M, H, I and S groups were higher than those in the C group. There were no significant differences in lactate and glycogen levels between the 5 groups. MMA 1000 μg/ml is much higher than the blood level (0.05–31.89 μg/ml) which was reported in clinical patients who had femoral prosthesis. Therefore, the direct contribution of MMA itself to cardiac depression may be less than the other factors such as embolism in clinical situations. Volatile anesthetics did not influence the deleterious effects of MMA on cardiac function and metabolism.
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alterations of cardiac function and metabolism in the rat Heart Lung Preparation by methyl methacrylate mma and their protection by ulinastatin
Masui. The Japanese journal of anesthesiology, 1995Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, M Kume, Atsushi Furuya, Teruo KumazawaAbstract:: We have assessed the deleterious effects of methyl methacrylate (MMA) on cardiac function and metabolism in the isolated Heart-Lung Preparation and their protection by ulinastatin. Twenty-four male Wistar rats were prepared for the Heart-Lung model. They were randomly divided into 3 groups. In the MMA (M) and ulinastatin (U) groups, MMA 1000 micrograms.ml-1 was administered 7 minutes after the start of perfusion. At the end of the experimental period, the Hearts were freeze-clamped and then myocardial high energy phosphates, lactate and glycogen were measured. Cardiac output decreased significantly in the M and U groups. Po2 of the perfusion blood in the M and U groups was significantly lower than that in the control (C) group. Myocardial ATP in the M and U groups was significantly lower than that in the C group. ADP and AMP in the M and U groups were higher than those in the C group. Although there was no significant difference in lactate levels among the 3 groups, glycogen in the U and C groups was significantly higher than that in the M group. MMA 1000 micrograms.ml-1 is much higher than the blood level (0.05-31.89 micrograms.ml-1) reported clinically in patients who had femoral prosthesis. Ulinastatin increased myocardial glycogen content which had been reduced by MMA. This may suggest that ulinastatin has a protective effect on Heart damaged by MMA.
Satoshi Kashimoto - One of the best experts on this subject based on the ideXlab platform.
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amrinone improves postischemic myocardial metabolism in the rat Heart Lung Preparation
Journal of Anesthesia, 1996Co-Authors: M Kume, Satoshi Kashimoto, Atsusi Furuya, Teruo KumazawaAbstract:Amrinone, a phosphodiesterase inhibitor, is a non-glycosidic noncatecholamine with both vasodilator and positive inotropic effects. We were interested in assessing the effect of amrinone on postischemic cardiac performance in the isolated Heart-Lung Preparation. Twenty-four male Wistar-ST rats were used. They were randomly divided into three groups. Amrinone, 10 μg·ml−1 or 100 μg·ml−1 was administered 8 min after the start of perfusion except in the control group. Ten minutes after the start of perfusion, all Hearts were made globally ischemic for 8 min. Subsequently, the Preparations were reperfused for 10 min. At the end of the experimental period, the Hearts were freeze-clamped, and then myocardial high-energy phosphates, lactate, pyruvate, and glycogen were measured. Hemodynamic parameters in all groups decreased significantly during ischemia. However, there were no significant differences among the groups. The myocardial ATP level in the 100 μg·ml−1 group was significantly higher than that in the control group. Adenosine diphosphate (ADP) and adenosine monophosphate (AMP) levels in the 100 μg·ml−1 group were significantly lower than those in the control group. Myocardial lactate, pyruvate, and glycogen levels were not significantly different among the groups. This result suggests that amrinone improves postischemic myocardial metabolism. Although we could not measure coronary flow, amrinone might increase coronary flow with direct coronary vasodilation which would have increased the myocardial ATP and energy charge levels.
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flumazenil does not antagonize the cardiac effects of midazolam in the isolated rat Heart Lung Preparation
Journal of Anesthesia, 1996Co-Authors: Toshihiro Nakamura, Satoshi Kashimoto, Akihiko Nonaka, Teruo KumazawaAbstract:We examined the effects of midazolam and flumazenil on cardiac function and metabolism in the isolated rat Heart-Lung Preparation. Wistar rats were divided into five groups (each group:n=8) as follows: (1) control (saline); (2) flumazenil (1.3×10−5M); (3) flumazenil (10−4M); (4) midazolam (60μg·ml−1); and (5) midazolam (60μg·ml−1) and flumazenil (1.3×10−5M). Systolic blood pressure and calculated left ventricular dP/dt maximum in the midazolam or midazolam conbined with flumazenil groups increased significantly in comparison with those in the control group. Heart rate in the midazolam group was lower than that in the control group. However, in the flumazenil group, there were no effects on the hemodynamics. There were no significant differences in the myocardial tissue concentration of ATP, lactate, and glycogen in all groups. In this study, midazolam decreased Heart rate; however, flumazenil had no effect on the Heart, nor did it antagonize the cardiac effects of midazolam. These results suggest that flumazenil has no effect on the peripheraltype benzodiazepine receptor of the myocardium.
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cardiac effects of methylmethacrylate in the rat Heart Lung Preparation with or without volatile anesthetics
Resuscitation, 1995Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, Akihiko Nonaka, Atsushi Furuya, Takashi Matsukawa, Teruo KumazawaAbstract:Abstract We have assessed the deleterious effects of methylmethacrylate (MMA) on cardiac function and metabolism in the isolated Heart-Lung Preparation with or without volatile anesthetics. Wistar rats were prepared for the Heart-Lung model. They were randomly divided into 5 groups as follows. (1) Control (C) group. (2) Cement (M) group; they received MMA. (3) Halothane (H) group; they received MMA and 1% halothane. (4) Isoflurane (I) group; they received MMA and 1.5% isoflurane. (5) Sevoflurane (S) group; they received MMA and 2.5% sevoflurane. MMA 1000 μg/ml was administered 7 min after the start of perfusion except in the C group. At the end of the experimental period, the Hearts were freeze-clamped and then myocardial high energy phosphates, lactate and glycogen were measured. Cardiac output in all groups but C group decreased significantly. PO 2 of the perfusion blood in the M, H, I and S groups was significantly lower than that in the C group. Myocardial ATP in the M, H, I and S groups was significantly lower than that in the C group. ADP and AMP in the M, H, I and S groups were higher than those in the C group. There were no significant differences in lactate and glycogen levels between the 5 groups. MMA 1000 μg/ml is much higher than the blood level (0.05–31.89 μg/ml) which was reported in clinical patients who had femoral prosthesis. Therefore, the direct contribution of MMA itself to cardiac depression may be less than the other factors such as embolism in clinical situations. Volatile anesthetics did not influence the deleterious effects of MMA on cardiac function and metabolism.
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alterations of cardiac function and metabolism in the rat Heart Lung Preparation by methyl methacrylate mma and their protection by ulinastatin
Masui. The Japanese journal of anesthesiology, 1995Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, M Kume, Atsushi Furuya, Teruo KumazawaAbstract:: We have assessed the deleterious effects of methyl methacrylate (MMA) on cardiac function and metabolism in the isolated Heart-Lung Preparation and their protection by ulinastatin. Twenty-four male Wistar rats were prepared for the Heart-Lung model. They were randomly divided into 3 groups. In the MMA (M) and ulinastatin (U) groups, MMA 1000 micrograms.ml-1 was administered 7 minutes after the start of perfusion. At the end of the experimental period, the Hearts were freeze-clamped and then myocardial high energy phosphates, lactate and glycogen were measured. Cardiac output decreased significantly in the M and U groups. Po2 of the perfusion blood in the M and U groups was significantly lower than that in the control (C) group. Myocardial ATP in the M and U groups was significantly lower than that in the C group. ADP and AMP in the M and U groups were higher than those in the C group. Although there was no significant difference in lactate levels among the 3 groups, glycogen in the U and C groups was significantly higher than that in the M group. MMA 1000 micrograms.ml-1 is much higher than the blood level (0.05-31.89 micrograms.ml-1) reported clinically in patients who had femoral prosthesis. Ulinastatin increased myocardial glycogen content which had been reduced by MMA. This may suggest that ulinastatin has a protective effect on Heart damaged by MMA.
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effects of artificial blood fc 43 emulsion on myocardial energy metabolism in the rat Heart Lung Preparation
BJA: British Journal of Anaesthesia, 1994Co-Authors: Satoshi Kashimoto, M Kume, T Oguchi, Akihiko Nonaka, T Nakamura, Teruo KumazawaAbstract:We have assessed the effects of artificial blood (FC-43 emulsion) on myocardial energy metabolism in the rat Heart-Lung Preparation. Animals were allocated to four groups ( n = 8 each group) according to the ratio of perfusion blood and FC-43 as follows: group 1 = control, perfusion blood only; group 2 = 50% FC-43; group 3 = 75% FC-43; and group 4 = 100% FC-43. Hearts were perfused initially to a cardiac output of 30 ml min −1 and mean arterial pressure of 50 mm Hg. Thirty minutes after perfusion, the Hearts were freeze-dried for 6 days. Myocardial high energy phosphates (ATP, ADP and AMP) were measured by high pressure liquid chromatography. Myocardial lactate and glycogen concentrations were measured by enzymatic methods. There were almost no significant differences in cardiac output, systolic pressure, right atrial pressure and Heart rate among the groups. Oxygen contents of the perfusate in all FC-43 groups were significantly lower than those in the control group. Myocardial ATP concentrations in rats receiving 50%, 75% and 100% FC-43 were significantly lower than those in the control group. In addition, myocardial ADP and AMP concentrations in rats receiving 75% and 100% FC-43 were significantly higher than those in the control group. Although there is adequate oxygen-carrying capacity in FC-43 to maintain cardiac function during perfusion, the energy levels in the Hearts perfused with FC-43 were lower than those in normal Hearts.
M Kume - One of the best experts on this subject based on the ideXlab platform.
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effects of cibenzoline on cardiac function and metabolism in the rat Heart Lung Preparation
European Journal of Anaesthesiology, 2001Co-Authors: Atsushi Furuya, M Kume, S Kashimoto, Teruo KumazawaAbstract:Summary Background and objective Although there is concern that cibenzoline, an antidysrhythmic drug for the treatment of ventricular and supraventricular dysrhythmias, may be associated with dose-dependent inhibition of myocardial contractility there are few reports about the relationship between myocardial metabolism and cardiac function when it is used. The present study was designed to investigate the effects of cibenzoline on cardiac function and metabolism. The effects of cibenzoline on cardiac function and myocardial metabolism were assessed in the isolated rat Heart–Lung Preparation. Methods Thirty-two male Wistar-ST rats were divided into four groups: control, and those to receive cibenzoline, either 300, 900 or 3000 ng mL−1. The cibenzoline was administered into the perfusate 5 min after the start of perfusion. Heart rates in the 3000 ng mL−1 group were significantly lower than those in the control group. Cardiac output in the 3000 ng mL−1 group at 15 and 30 min was significantly lower than in the control group. In all groups, values for %LV dP/dt max (the ratio of values at each time to those at 5 min) at 20, 25, 30 min were significantly higher than at 5 min. Myocardial adenosine triphosphate concentration in the 3000 ng mL−1 group was significantly lower than in controls. There was no difference between groups in the lactate/pyruvate ratio. Conclusion The therapeutic range of cibenzoline has few effects on cardiac function and metabolism, although concentrations 10 times greater may cause a deterioration in myocardial metabolism.
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amrinone improves postischemic myocardial metabolism in the rat Heart Lung Preparation
Journal of Anesthesia, 1996Co-Authors: M Kume, Satoshi Kashimoto, Atsusi Furuya, Teruo KumazawaAbstract:Amrinone, a phosphodiesterase inhibitor, is a non-glycosidic noncatecholamine with both vasodilator and positive inotropic effects. We were interested in assessing the effect of amrinone on postischemic cardiac performance in the isolated Heart-Lung Preparation. Twenty-four male Wistar-ST rats were used. They were randomly divided into three groups. Amrinone, 10 μg·ml−1 or 100 μg·ml−1 was administered 8 min after the start of perfusion except in the control group. Ten minutes after the start of perfusion, all Hearts were made globally ischemic for 8 min. Subsequently, the Preparations were reperfused for 10 min. At the end of the experimental period, the Hearts were freeze-clamped, and then myocardial high-energy phosphates, lactate, pyruvate, and glycogen were measured. Hemodynamic parameters in all groups decreased significantly during ischemia. However, there were no significant differences among the groups. The myocardial ATP level in the 100 μg·ml−1 group was significantly higher than that in the control group. Adenosine diphosphate (ADP) and adenosine monophosphate (AMP) levels in the 100 μg·ml−1 group were significantly lower than those in the control group. Myocardial lactate, pyruvate, and glycogen levels were not significantly different among the groups. This result suggests that amrinone improves postischemic myocardial metabolism. Although we could not measure coronary flow, amrinone might increase coronary flow with direct coronary vasodilation which would have increased the myocardial ATP and energy charge levels.
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alterations of cardiac function and metabolism in the rat Heart Lung Preparation by methyl methacrylate mma and their protection by ulinastatin
Masui. The Japanese journal of anesthesiology, 1995Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, M Kume, Atsushi Furuya, Teruo KumazawaAbstract:: We have assessed the deleterious effects of methyl methacrylate (MMA) on cardiac function and metabolism in the isolated Heart-Lung Preparation and their protection by ulinastatin. Twenty-four male Wistar rats were prepared for the Heart-Lung model. They were randomly divided into 3 groups. In the MMA (M) and ulinastatin (U) groups, MMA 1000 micrograms.ml-1 was administered 7 minutes after the start of perfusion. At the end of the experimental period, the Hearts were freeze-clamped and then myocardial high energy phosphates, lactate and glycogen were measured. Cardiac output decreased significantly in the M and U groups. Po2 of the perfusion blood in the M and U groups was significantly lower than that in the control (C) group. Myocardial ATP in the M and U groups was significantly lower than that in the C group. ADP and AMP in the M and U groups were higher than those in the C group. Although there was no significant difference in lactate levels among the 3 groups, glycogen in the U and C groups was significantly higher than that in the M group. MMA 1000 micrograms.ml-1 is much higher than the blood level (0.05-31.89 micrograms.ml-1) reported clinically in patients who had femoral prosthesis. Ulinastatin increased myocardial glycogen content which had been reduced by MMA. This may suggest that ulinastatin has a protective effect on Heart damaged by MMA.
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effects of artificial blood fc 43 emulsion on myocardial energy metabolism in the rat Heart Lung Preparation
BJA: British Journal of Anaesthesia, 1994Co-Authors: Satoshi Kashimoto, M Kume, T Oguchi, Akihiko Nonaka, T Nakamura, Teruo KumazawaAbstract:We have assessed the effects of artificial blood (FC-43 emulsion) on myocardial energy metabolism in the rat Heart-Lung Preparation. Animals were allocated to four groups ( n = 8 each group) according to the ratio of perfusion blood and FC-43 as follows: group 1 = control, perfusion blood only; group 2 = 50% FC-43; group 3 = 75% FC-43; and group 4 = 100% FC-43. Hearts were perfused initially to a cardiac output of 30 ml min −1 and mean arterial pressure of 50 mm Hg. Thirty minutes after perfusion, the Hearts were freeze-dried for 6 days. Myocardial high energy phosphates (ATP, ADP and AMP) were measured by high pressure liquid chromatography. Myocardial lactate and glycogen concentrations were measured by enzymatic methods. There were almost no significant differences in cardiac output, systolic pressure, right atrial pressure and Heart rate among the groups. Oxygen contents of the perfusate in all FC-43 groups were significantly lower than those in the control group. Myocardial ATP concentrations in rats receiving 50%, 75% and 100% FC-43 were significantly lower than those in the control group. In addition, myocardial ADP and AMP concentrations in rats receiving 75% and 100% FC-43 were significantly higher than those in the control group. Although there is adequate oxygen-carrying capacity in FC-43 to maintain cardiac function during perfusion, the energy levels in the Hearts perfused with FC-43 were lower than those in normal Hearts.
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effects and interaction of nicardipine and volatile anesthetics in the rat Heart Lung Preparation
Journal of Anesthesia, 1994Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, M Kume, Akihiko Nonaka, Teruo KumazawaAbstract:The effects of the calcium channel blocker nicardipine (N) and the volatile anesthetics halothane (H), enflurane (E), isoflurane (I), and sevoflurane (S) on myocardial metabolism after postischemic reperfusion were assessed in the isolated rat Heart-Lung Preparation. Wistar-ST rats were randomly divided into six groups (each groupn=9) as follows: control (C) group, no drugs; N group, N (100 ng·ml−1); H group, 1% H and N; E group, 2.2% E and N; I group, 1.5% I and N; and the S group, 3.3% S and N. In the presence of the volatile anesthetics, the Preparations were perfused for 10 min, made globally ischemic for 8 min, and then reperfused for 10 min. N 100 ng·ml−1 was administered 5 min before ischemia except in the C group. Three Hearts in the C and H groups (eachn=9) and one Heart in the E group (n=9) failed to recover from ischemia. The recovery times in the N, I and S groups were significantly shorter than controls. Although there was no significant difference in myocardial lactate concentrations among the groups, ATP content in the N, H, E, I and S groups was significantly higher than in controls. Glycogen content in the N, E, I and S groups was also significantly higher than in controls. These results suggest that N improves myocardial recovery from ischemia; however, in the presence of H or E it may cause significant myocardial depression.
Toshihiro Nakamura - One of the best experts on this subject based on the ideXlab platform.
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flumazenil does not antagonize the cardiac effects of midazolam in the isolated rat Heart Lung Preparation
Journal of Anesthesia, 1996Co-Authors: Toshihiro Nakamura, Satoshi Kashimoto, Akihiko Nonaka, Teruo KumazawaAbstract:We examined the effects of midazolam and flumazenil on cardiac function and metabolism in the isolated rat Heart-Lung Preparation. Wistar rats were divided into five groups (each group:n=8) as follows: (1) control (saline); (2) flumazenil (1.3×10−5M); (3) flumazenil (10−4M); (4) midazolam (60μg·ml−1); and (5) midazolam (60μg·ml−1) and flumazenil (1.3×10−5M). Systolic blood pressure and calculated left ventricular dP/dt maximum in the midazolam or midazolam conbined with flumazenil groups increased significantly in comparison with those in the control group. Heart rate in the midazolam group was lower than that in the control group. However, in the flumazenil group, there were no effects on the hemodynamics. There were no significant differences in the myocardial tissue concentration of ATP, lactate, and glycogen in all groups. In this study, midazolam decreased Heart rate; however, flumazenil had no effect on the Heart, nor did it antagonize the cardiac effects of midazolam. These results suggest that flumazenil has no effect on the peripheraltype benzodiazepine receptor of the myocardium.
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cardiac effects of methylmethacrylate in the rat Heart Lung Preparation with or without volatile anesthetics
Resuscitation, 1995Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, Akihiko Nonaka, Atsushi Furuya, Takashi Matsukawa, Teruo KumazawaAbstract:Abstract We have assessed the deleterious effects of methylmethacrylate (MMA) on cardiac function and metabolism in the isolated Heart-Lung Preparation with or without volatile anesthetics. Wistar rats were prepared for the Heart-Lung model. They were randomly divided into 5 groups as follows. (1) Control (C) group. (2) Cement (M) group; they received MMA. (3) Halothane (H) group; they received MMA and 1% halothane. (4) Isoflurane (I) group; they received MMA and 1.5% isoflurane. (5) Sevoflurane (S) group; they received MMA and 2.5% sevoflurane. MMA 1000 μg/ml was administered 7 min after the start of perfusion except in the C group. At the end of the experimental period, the Hearts were freeze-clamped and then myocardial high energy phosphates, lactate and glycogen were measured. Cardiac output in all groups but C group decreased significantly. PO 2 of the perfusion blood in the M, H, I and S groups was significantly lower than that in the C group. Myocardial ATP in the M, H, I and S groups was significantly lower than that in the C group. ADP and AMP in the M, H, I and S groups were higher than those in the C group. There were no significant differences in lactate and glycogen levels between the 5 groups. MMA 1000 μg/ml is much higher than the blood level (0.05–31.89 μg/ml) which was reported in clinical patients who had femoral prosthesis. Therefore, the direct contribution of MMA itself to cardiac depression may be less than the other factors such as embolism in clinical situations. Volatile anesthetics did not influence the deleterious effects of MMA on cardiac function and metabolism.
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alterations of cardiac function and metabolism in the rat Heart Lung Preparation by methyl methacrylate mma and their protection by ulinastatin
Masui. The Japanese journal of anesthesiology, 1995Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, M Kume, Atsushi Furuya, Teruo KumazawaAbstract:: We have assessed the deleterious effects of methyl methacrylate (MMA) on cardiac function and metabolism in the isolated Heart-Lung Preparation and their protection by ulinastatin. Twenty-four male Wistar rats were prepared for the Heart-Lung model. They were randomly divided into 3 groups. In the MMA (M) and ulinastatin (U) groups, MMA 1000 micrograms.ml-1 was administered 7 minutes after the start of perfusion. At the end of the experimental period, the Hearts were freeze-clamped and then myocardial high energy phosphates, lactate and glycogen were measured. Cardiac output decreased significantly in the M and U groups. Po2 of the perfusion blood in the M and U groups was significantly lower than that in the control (C) group. Myocardial ATP in the M and U groups was significantly lower than that in the C group. ADP and AMP in the M and U groups were higher than those in the C group. Although there was no significant difference in lactate levels among the 3 groups, glycogen in the U and C groups was significantly higher than that in the M group. MMA 1000 micrograms.ml-1 is much higher than the blood level (0.05-31.89 micrograms.ml-1) reported clinically in patients who had femoral prosthesis. Ulinastatin increased myocardial glycogen content which had been reduced by MMA. This may suggest that ulinastatin has a protective effect on Heart damaged by MMA.
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effects and interaction of nicardipine and volatile anesthetics in the rat Heart Lung Preparation
Journal of Anesthesia, 1994Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, M Kume, Akihiko Nonaka, Teruo KumazawaAbstract:The effects of the calcium channel blocker nicardipine (N) and the volatile anesthetics halothane (H), enflurane (E), isoflurane (I), and sevoflurane (S) on myocardial metabolism after postischemic reperfusion were assessed in the isolated rat Heart-Lung Preparation. Wistar-ST rats were randomly divided into six groups (each groupn=9) as follows: control (C) group, no drugs; N group, N (100 ng·ml−1); H group, 1% H and N; E group, 2.2% E and N; I group, 1.5% I and N; and the S group, 3.3% S and N. In the presence of the volatile anesthetics, the Preparations were perfused for 10 min, made globally ischemic for 8 min, and then reperfused for 10 min. N 100 ng·ml−1 was administered 5 min before ischemia except in the C group. Three Hearts in the C and H groups (eachn=9) and one Heart in the E group (n=9) failed to recover from ischemia. The recovery times in the N, I and S groups were significantly shorter than controls. Although there was no significant difference in myocardial lactate concentrations among the groups, ATP content in the N, H, E, I and S groups was significantly higher than in controls. Glycogen content in the N, E, I and S groups was also significantly higher than in controls. These results suggest that N improves myocardial recovery from ischemia; however, in the presence of H or E it may cause significant myocardial depression.
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Protective effects of prostaglandin I2 analogues on CPK release in rat's Heart-Lung Preparation.
Journal of Anesthesia, 1991Co-Authors: Satoshi Kashimoto, Toshihiro Nakamura, Takeshi Oguchi, Kume M, Teruo KumazawaAbstract:The effects of prostaglandin I2 analogues (PGI2-a: op-41483 and op-2507) on oxygen toxicity during hyperoxic perfusion were evaluated in an experiment on isolated rat Heart Lung Preparation, with the release of creatine phosphokinase (CPK) in the perfusate blood. There were no significant differences in Heart rate and right atrial pressure between PGI2-a treated and untreated Hearts. The CPK release from the Heart with oxygen was significantly higher than that of the air (P
James T Diehl - One of the best experts on this subject based on the ideXlab platform.
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Leukocyte redistribution and eicosanoid changes during the autoperfused working Heart-Lung Preparation.
Journal of Investigative Surgery, 2009Co-Authors: Christopher M Genco, Raymond J Connolly, Myron B Peterson, Eugene A Bernstein, Xi Zhang, Richard J Cleveland, Kenneth H. Somerville, James T DiehlAbstract:We studied the role of leukocyte redistribution and eicosanoid changes in the early stages of instituting 16 rabbit autoperfused working Heart-Lung Preparations (A WHLP). Physiological changes occurring during the transition from the intact animal to the A WHLP may determine the survival and viability of the organ blocks for transplantation. White blood cell (WBC) count decreased from 5160/μL to 1430/μL (P
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granulocyte sequestration and early failure in the autoperfused Heart Lung Preparation
The Annals of Thoracic Surgery, 1992Co-Authors: Christopher M Genco, Raymond J Connolly, Myron B Peterson, Eugene A Bernstein, Karen Ramberg, Xi Zhang, Richard J Cleveland, James T DiehlAbstract:Abstract We investigated the role of pulmonary granulocyte sequestration in the development of early failure of the autoperfused working Heart-Lung Preparation. A significant decline in the total circulating leukocyte count in 21 Preparations at 60 minutes of perfusion (5.0 to 1.4 × 10 3 /μL; 28% of baseline; p p 3 /μL ± standard error of the mean). Postflush Lung activity was significantly increased in group I versus group II (I, 3,751 ± 566; II, 1,867 ± 532; p p
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leukocyte redistribution and eicosanoid changes during the autoperfused working Heart Lung Preparation
Journal of Investigative Surgery, 1991Co-Authors: Christopher M Genco, Raymond J Connolly, Myron B Peterson, Eugene A Bernstein, Xi Zhang, Richard J Cleveland, Kenneth Somerville, James T DiehlAbstract:We studied the role of leukocyte redistribution and eicosanoid changes in the early stages of instituting 16 rabbit autoperfused working Heart-Lung Preparations (A WHLP). Physiological changes occurring during the transition from the intact animal to the A WHLP may determine the survival and viability of the organ blocks for transplantation. White blood cell (WBC) count decreased from 5160/μL to 1430/μL (P <. 01) at 60 min of autoperfusion. Differential WBC counts performed in ten of these A WHLP revealed a 63% decrease in lymphocyte count and an 88% decrease in the granulocyte count at 60 min. Thus, the predominant leukocyte remaining in the circulation was the lymphocyte. Blood samples were collected from the intact animal and from the A WHLP for assay of the stable metabolites of thromboxane A2 (TxA2) and prostacyclin (PGI2). Transition from the in situ Heart-Lung block to the in vitro A WHLP stage caused significant changes in these metabolites. The PGI, metabolite 6-ketoprostaglandin F1, (6KPGF1a) in...
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extended ex vivo preservation of the Heart and Lungs effects of acellular oxygen carrying perfusates and indomethacin on the autoperfused working Heart Lung Preparation
The Journal of Thoracic and Cardiovascular Surgery, 1990Co-Authors: E Kaplan, Raymond J Connolly, Myron B Peterson, James T Diehl, Kenneth Somerville, Benedict D T Daly, Amiel G Cooper, Shawn D Seiler, Richard J ClevelandAbstract:: The autoperfused working Heart-Lung Preparation has been proposed as a method for long-term Heart-Lung preservation. We investigated the effects of acellular oxygen-carrying perfusates (study 1) and the effect of donor pretreatment with indomethacin (study 2) on the working ex vivo Heart-Lung block. In study 1 perfusion with stroma-fee hemoglobin resulted in significantly reduced survival (118 +/- 46 minutes) compared with autologous blood (561 +/- 125 minutes, p less than 0.05) or perfluorocarbon (438 +/- 114 minutes, p less than 0.05). Decrease in survival with stroma-free hemoglobin perfusate is associated with a marked decrease in left ventricular performance and a significant increase in pulmonary vascular resistance. Perfusion with autologous blood is associated with a significant increase in pulmonary vascular resistance after 240 minutes of explantation, which is significantly delayed by perfusion with perfluorocarbon. Perfusion for 6 hours with blood pretreated with indomethacin (study 2) resulted in a decrease in the concentration of prostacyclin and thromboxane A2 metabolites but an increase in the prostaglandin/thromboxane A2 metabolite ratio. This is associated with abrogation of the increase in pulmonary vascular resistance (12,787 +/- 1682 dynes/sec/cm-5, T = 0; 13,134 +/- 2654 dynes/sec/cm-5, T = 360 minutes) observed in Preparations perfused with autologous blood (13,194 +/- 1942 dynes/sec/cm-5, T = 0; 24,768 +/- 3325 dynes/sec/cm-5, T = 360 minutes, p less than 0.05). We conclude that alteration of the cellular and humoral components of autologous blood may prove advantageous for increasing the utility of the autoperfused working Heart-Lung Preparation as a preservation technique.
