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Kouichiro Tsuge - One of the best experts on this subject based on the ideXlab platform.

  • Species identification of white false Hellebore (Veratrum album subsp. oxysepalum) by loop-mediated isothermal amplification (LAMP)
    Forensic Toxicology, 2019
    Co-Authors: Hitomi S. Kikkawa, Kouichiro Tsuge, Masako Aragane
    Abstract:

    Purpose Food poisoning is frequently caused by the accidental ingestion of toxic plants with strong morphological similarities to edible plants. The plant, false helleborine ( Veratrum album ), is commonly involved in such incidents. Cases of poisoning by toxic plants require rapid and accurate identification for appropriate medical treatment or forensic investigation in the event of death. However, morphological examinations require experience in systematic botany, and chemical analysis of natural compounds can be challenging. This study aimed to develop a sensitive, rapid, and specific method for identifying V. album and V. stamineum . Methods We developed and optimized a loop-mediated isothermal amplification (LAMP) detection method. Total genomic DNA was extracted from approximately 10 mg (dry weight) of leaf from 26 specimens comprising the target plants, allied plants, and other causative plants of poisoning. These samples were analyzed the LAMP assay, and mixed samples, cooked samples, and simulated gastric contents were also tested. Results The limit of detection of our LAMP assay was 0.08 ng of target DNA, and the assay was specific to V. album and V. stamineum . The LAMP assay could be performed in approximately 30 min. V. album was also successfully identified in mixed samples, cooked samples, and simulated gastric contents. In addition, the LAMP assay signal could be effectively assessed with the naked eye. Conclusions The present LAMP assay is a highly sensitive, rapid, and specific method for identifying V. album and V. stamineum .

  • Species identification of white false Hellebore ( Veratrum album subsp. oxysepalum ) by loop-mediated isothermal amplification (LAMP)
    Forensic Toxicology, 2019
    Co-Authors: Hitomi S. Kikkawa, Masako Aragane, Kouichiro Tsuge
    Abstract:

    Food poisoning is frequently caused by the accidental ingestion of toxic plants with strong morphological similarities to edible plants. The plant, false helleborine (Veratrum album), is commonly involved in such incidents. Cases of poisoning by toxic plants require rapid and accurate identification for appropriate medical treatment or forensic investigation in the event of death. However, morphological examinations require experience in systematic botany, and chemical analysis of natural compounds can be challenging. This study aimed to develop a sensitive, rapid, and specific method for identifying V. album and V. stamineum. We developed and optimized a loop-mediated isothermal amplification (LAMP) detection method. Total genomic DNA was extracted from approximately 10 mg (dry weight) of leaf from 26 specimens comprising the target plants, allied plants, and other causative plants of poisoning. These samples were analyzed the LAMP assay, and mixed samples, cooked samples, and simulated gastric contents were also tested. The limit of detection of our LAMP assay was 0.08 ng of target DNA, and the assay was specific to V. album and V. stamineum. The LAMP assay could be performed in approximately 30 min. V. album was also successfully identified in mixed samples, cooked samples, and simulated gastric contents. In addition, the LAMP assay signal could be effectively assessed with the naked eye. The present LAMP assay is a highly sensitive, rapid, and specific method for identifying V. album and V. stamineum.

  • Species identification of white false Hellebore (Veratrum album subsp. oxysepalum) using real-time PCR
    Forensic science international, 2017
    Co-Authors: Hitomi S. Kikkawa, Kouichiro Tsuge, Satoshi Kubota, Masako Aragane, Hikoto Ohta, Ritsuko Sugita
    Abstract:

    Food poisoning is frequently caused by the accidental ingestion of toxic plants that possess strong morphological similarities to edible plants. False helleborine (Veratrum album) is one of the most common plants involved in such accidents. In cases of poisoning by toxic plants, rapid and accurate identification, usually based on the morphological or chemical analysis of plant parts, is required for appropriate medical treatment or forensic investigation. However, morphological examinations require experience in systematic botany because the samples are fragmentary, and chemical analysis of natural compounds can be difficult. In this study, we developed a TaqMan real-time PCR method using trnH-psbA and trnL-trnF that could be carried out in 30-60min. The lower detection limit was less than 10pg of DNA and the primer sets were specific to V. album and Veratrum stamineum. Mixed samples, cooked samples, and simulated gastric contents were successfully identified, and a multiplex assay of two regions was also possible. These results indicate that the TaqMan real-time PCR analysis is a very effective method to detect small samples of V. album and V. stamineum accurately and rapidly in poisoning cases.

Hitomi S. Kikkawa - One of the best experts on this subject based on the ideXlab platform.

  • Species identification of white false Hellebore (Veratrum album subsp. oxysepalum) by loop-mediated isothermal amplification (LAMP)
    Forensic Toxicology, 2019
    Co-Authors: Hitomi S. Kikkawa, Kouichiro Tsuge, Masako Aragane
    Abstract:

    Purpose Food poisoning is frequently caused by the accidental ingestion of toxic plants with strong morphological similarities to edible plants. The plant, false helleborine ( Veratrum album ), is commonly involved in such incidents. Cases of poisoning by toxic plants require rapid and accurate identification for appropriate medical treatment or forensic investigation in the event of death. However, morphological examinations require experience in systematic botany, and chemical analysis of natural compounds can be challenging. This study aimed to develop a sensitive, rapid, and specific method for identifying V. album and V. stamineum . Methods We developed and optimized a loop-mediated isothermal amplification (LAMP) detection method. Total genomic DNA was extracted from approximately 10 mg (dry weight) of leaf from 26 specimens comprising the target plants, allied plants, and other causative plants of poisoning. These samples were analyzed the LAMP assay, and mixed samples, cooked samples, and simulated gastric contents were also tested. Results The limit of detection of our LAMP assay was 0.08 ng of target DNA, and the assay was specific to V. album and V. stamineum . The LAMP assay could be performed in approximately 30 min. V. album was also successfully identified in mixed samples, cooked samples, and simulated gastric contents. In addition, the LAMP assay signal could be effectively assessed with the naked eye. Conclusions The present LAMP assay is a highly sensitive, rapid, and specific method for identifying V. album and V. stamineum .

  • Species identification of white false Hellebore ( Veratrum album subsp. oxysepalum ) by loop-mediated isothermal amplification (LAMP)
    Forensic Toxicology, 2019
    Co-Authors: Hitomi S. Kikkawa, Masako Aragane, Kouichiro Tsuge
    Abstract:

    Food poisoning is frequently caused by the accidental ingestion of toxic plants with strong morphological similarities to edible plants. The plant, false helleborine (Veratrum album), is commonly involved in such incidents. Cases of poisoning by toxic plants require rapid and accurate identification for appropriate medical treatment or forensic investigation in the event of death. However, morphological examinations require experience in systematic botany, and chemical analysis of natural compounds can be challenging. This study aimed to develop a sensitive, rapid, and specific method for identifying V. album and V. stamineum. We developed and optimized a loop-mediated isothermal amplification (LAMP) detection method. Total genomic DNA was extracted from approximately 10 mg (dry weight) of leaf from 26 specimens comprising the target plants, allied plants, and other causative plants of poisoning. These samples were analyzed the LAMP assay, and mixed samples, cooked samples, and simulated gastric contents were also tested. The limit of detection of our LAMP assay was 0.08 ng of target DNA, and the assay was specific to V. album and V. stamineum. The LAMP assay could be performed in approximately 30 min. V. album was also successfully identified in mixed samples, cooked samples, and simulated gastric contents. In addition, the LAMP assay signal could be effectively assessed with the naked eye. The present LAMP assay is a highly sensitive, rapid, and specific method for identifying V. album and V. stamineum.

  • Species identification of white false Hellebore (Veratrum album subsp. oxysepalum) using real-time PCR
    Forensic science international, 2017
    Co-Authors: Hitomi S. Kikkawa, Kouichiro Tsuge, Satoshi Kubota, Masako Aragane, Hikoto Ohta, Ritsuko Sugita
    Abstract:

    Food poisoning is frequently caused by the accidental ingestion of toxic plants that possess strong morphological similarities to edible plants. False helleborine (Veratrum album) is one of the most common plants involved in such accidents. In cases of poisoning by toxic plants, rapid and accurate identification, usually based on the morphological or chemical analysis of plant parts, is required for appropriate medical treatment or forensic investigation. However, morphological examinations require experience in systematic botany because the samples are fragmentary, and chemical analysis of natural compounds can be difficult. In this study, we developed a TaqMan real-time PCR method using trnH-psbA and trnL-trnF that could be carried out in 30-60min. The lower detection limit was less than 10pg of DNA and the primer sets were specific to V. album and Veratrum stamineum. Mixed samples, cooked samples, and simulated gastric contents were successfully identified, and a multiplex assay of two regions was also possible. These results indicate that the TaqMan real-time PCR analysis is a very effective method to detect small samples of V. album and V. stamineum accurately and rapidly in poisoning cases.

Miran Brvar - One of the best experts on this subject based on the ideXlab platform.

  • accidental poisoning with veratrum album mistaken for wild garlic allium ursinum
    Clinical Toxicology, 2010
    Co-Authors: Irene Gilotta, Miran Brvar
    Abstract:

    Introduction. Veratrum album (white or false Hellebore) is a poisonous plant containing steroidal alkaloids that cause nausea, vomiting, headache, visual disturbances, paresthesia, dizziness, bradycardia, atrioventricular block, hypotension, and syncope. It is regularly mistaken for Gentiana lutea (yellow gentian). We report accidental poisoning with V. album mistaken for Allium ursinum (wild garlic), a wild plant used in soups and salads in Central Europe. Case series. Four adults (24–45 years) accidentally ingested V. album mistaken for A. ursinum in self-prepared salads and soups. Within 15–30 min of ingestion they developed nausea, vomiting, and abdominal pain. At the same time dizziness, tingling, dimmed and jumping vision, transient blindness, and confusion appeared. On arrival at the ED, all patients had sinus bradycardia and hypotension. Following treatment the patients were discharged well 24–48 h after ingestion. Conclusion. In patients presenting with gastrointestinal, neurological, and cardiov...

  • accidental poisoning with veratrum album mistaken for wild garlic allium ursinum
    Clinical Toxicology, 2010
    Co-Authors: Irene Gilotta, Miran Brvar
    Abstract:

    Introduction. Veratrum album (white or false Hellebore) is a poisonous plant containing steroidal alkaloids that cause nausea, vomiting, headache, visual disturbances, paresthesia, dizziness, bradycardia, atrioventricular block, hypotension, and syncope. It is regularly mistaken for Gentiana lutea (yellow gentian). We report accidental poisoning with V. album mistaken for Allium ursinum (wild garlic), a wild plant used in soups and salads in Central Europe. Case series. Four adults (24–45 years) accidentally ingested V. album mistaken for A. ursinum in self-prepared salads and soups. Within 15–30 min of ingestion they developed nausea, vomiting, and abdominal pain. At the same time dizziness, tingling, dimmed and jumping vision, transient blindness, and confusion appeared. On arrival at the ED, all patients had sinus bradycardia and hypotension. Following treatment the patients were discharged well 24–48 h after ingestion. Conclusion. In patients presenting with gastrointestinal, neurological, and cardiov...

Masako Aragane - One of the best experts on this subject based on the ideXlab platform.

  • Species identification of white false Hellebore (Veratrum album subsp. oxysepalum) by loop-mediated isothermal amplification (LAMP)
    Forensic Toxicology, 2019
    Co-Authors: Hitomi S. Kikkawa, Kouichiro Tsuge, Masako Aragane
    Abstract:

    Purpose Food poisoning is frequently caused by the accidental ingestion of toxic plants with strong morphological similarities to edible plants. The plant, false helleborine ( Veratrum album ), is commonly involved in such incidents. Cases of poisoning by toxic plants require rapid and accurate identification for appropriate medical treatment or forensic investigation in the event of death. However, morphological examinations require experience in systematic botany, and chemical analysis of natural compounds can be challenging. This study aimed to develop a sensitive, rapid, and specific method for identifying V. album and V. stamineum . Methods We developed and optimized a loop-mediated isothermal amplification (LAMP) detection method. Total genomic DNA was extracted from approximately 10 mg (dry weight) of leaf from 26 specimens comprising the target plants, allied plants, and other causative plants of poisoning. These samples were analyzed the LAMP assay, and mixed samples, cooked samples, and simulated gastric contents were also tested. Results The limit of detection of our LAMP assay was 0.08 ng of target DNA, and the assay was specific to V. album and V. stamineum . The LAMP assay could be performed in approximately 30 min. V. album was also successfully identified in mixed samples, cooked samples, and simulated gastric contents. In addition, the LAMP assay signal could be effectively assessed with the naked eye. Conclusions The present LAMP assay is a highly sensitive, rapid, and specific method for identifying V. album and V. stamineum .

  • Species identification of white false Hellebore ( Veratrum album subsp. oxysepalum ) by loop-mediated isothermal amplification (LAMP)
    Forensic Toxicology, 2019
    Co-Authors: Hitomi S. Kikkawa, Masako Aragane, Kouichiro Tsuge
    Abstract:

    Food poisoning is frequently caused by the accidental ingestion of toxic plants with strong morphological similarities to edible plants. The plant, false helleborine (Veratrum album), is commonly involved in such incidents. Cases of poisoning by toxic plants require rapid and accurate identification for appropriate medical treatment or forensic investigation in the event of death. However, morphological examinations require experience in systematic botany, and chemical analysis of natural compounds can be challenging. This study aimed to develop a sensitive, rapid, and specific method for identifying V. album and V. stamineum. We developed and optimized a loop-mediated isothermal amplification (LAMP) detection method. Total genomic DNA was extracted from approximately 10 mg (dry weight) of leaf from 26 specimens comprising the target plants, allied plants, and other causative plants of poisoning. These samples were analyzed the LAMP assay, and mixed samples, cooked samples, and simulated gastric contents were also tested. The limit of detection of our LAMP assay was 0.08 ng of target DNA, and the assay was specific to V. album and V. stamineum. The LAMP assay could be performed in approximately 30 min. V. album was also successfully identified in mixed samples, cooked samples, and simulated gastric contents. In addition, the LAMP assay signal could be effectively assessed with the naked eye. The present LAMP assay is a highly sensitive, rapid, and specific method for identifying V. album and V. stamineum.

  • Species identification of white false Hellebore (Veratrum album subsp. oxysepalum) using real-time PCR
    Forensic science international, 2017
    Co-Authors: Hitomi S. Kikkawa, Kouichiro Tsuge, Satoshi Kubota, Masako Aragane, Hikoto Ohta, Ritsuko Sugita
    Abstract:

    Food poisoning is frequently caused by the accidental ingestion of toxic plants that possess strong morphological similarities to edible plants. False helleborine (Veratrum album) is one of the most common plants involved in such accidents. In cases of poisoning by toxic plants, rapid and accurate identification, usually based on the morphological or chemical analysis of plant parts, is required for appropriate medical treatment or forensic investigation. However, morphological examinations require experience in systematic botany because the samples are fragmentary, and chemical analysis of natural compounds can be difficult. In this study, we developed a TaqMan real-time PCR method using trnH-psbA and trnL-trnF that could be carried out in 30-60min. The lower detection limit was less than 10pg of DNA and the primer sets were specific to V. album and Veratrum stamineum. Mixed samples, cooked samples, and simulated gastric contents were successfully identified, and a multiplex assay of two regions was also possible. These results indicate that the TaqMan real-time PCR analysis is a very effective method to detect small samples of V. album and V. stamineum accurately and rapidly in poisoning cases.

Irene Gilotta - One of the best experts on this subject based on the ideXlab platform.

  • accidental poisoning with veratrum album mistaken for wild garlic allium ursinum
    Clinical Toxicology, 2010
    Co-Authors: Irene Gilotta, Miran Brvar
    Abstract:

    Introduction. Veratrum album (white or false Hellebore) is a poisonous plant containing steroidal alkaloids that cause nausea, vomiting, headache, visual disturbances, paresthesia, dizziness, bradycardia, atrioventricular block, hypotension, and syncope. It is regularly mistaken for Gentiana lutea (yellow gentian). We report accidental poisoning with V. album mistaken for Allium ursinum (wild garlic), a wild plant used in soups and salads in Central Europe. Case series. Four adults (24–45 years) accidentally ingested V. album mistaken for A. ursinum in self-prepared salads and soups. Within 15–30 min of ingestion they developed nausea, vomiting, and abdominal pain. At the same time dizziness, tingling, dimmed and jumping vision, transient blindness, and confusion appeared. On arrival at the ED, all patients had sinus bradycardia and hypotension. Following treatment the patients were discharged well 24–48 h after ingestion. Conclusion. In patients presenting with gastrointestinal, neurological, and cardiov...

  • accidental poisoning with veratrum album mistaken for wild garlic allium ursinum
    Clinical Toxicology, 2010
    Co-Authors: Irene Gilotta, Miran Brvar
    Abstract:

    Introduction. Veratrum album (white or false Hellebore) is a poisonous plant containing steroidal alkaloids that cause nausea, vomiting, headache, visual disturbances, paresthesia, dizziness, bradycardia, atrioventricular block, hypotension, and syncope. It is regularly mistaken for Gentiana lutea (yellow gentian). We report accidental poisoning with V. album mistaken for Allium ursinum (wild garlic), a wild plant used in soups and salads in Central Europe. Case series. Four adults (24–45 years) accidentally ingested V. album mistaken for A. ursinum in self-prepared salads and soups. Within 15–30 min of ingestion they developed nausea, vomiting, and abdominal pain. At the same time dizziness, tingling, dimmed and jumping vision, transient blindness, and confusion appeared. On arrival at the ED, all patients had sinus bradycardia and hypotension. Following treatment the patients were discharged well 24–48 h after ingestion. Conclusion. In patients presenting with gastrointestinal, neurological, and cardiov...