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Anton Hartmann - One of the best experts on this subject based on the ideXlab platform.
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35 The Family Oxalobacteraceae
2014Co-Authors: Luc Felicianus Marie Rouws, Michael Schmid, Anton HartmannAbstract:Oxalobacteraceae is a family within the order Burkholderiales in the subclass of Betaproteobacteria which contains the 13 genera: Collimonas, Duganella, Glaciimonas, Herbaspirillum, Herminiimonas, Janthinobacterium, Massilia, NoviHerbaspirillum, Oxalicibacterium, Oxalobacter, Pseudoduganella, Telluria and Undibacterium. According to the 16S rRNA gene sequence similarities of the type species, the genera Herbaspirillum, NoviHerbaspirillum, Collimonas, Glaciimonas, Oxalobacter, and Oxalicibacterium as well as the genera Telluria, Massilia, Duganella, Pseudoduganella, Janthinobacterium, Undibacterium, and Herminiimonas appear more closely related withinthefamilyOxalobacteraceae.Themembersofthefamilyare heterotrophic and nonspore-forming gram-negative bacteria; they are mesophilic with the exception of some psychrophilic species.
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The genome of the endophytic bacterium H. frisingense GSF30 T identifies diverse strategies in the Herbaspirillum genus to interact with plants
Frontiers in microbiology, 2013Co-Authors: Daniel Straub, Anton Hartmann, Michael Rothballer, Uwe LudewigAbstract:The diazotrophic, bacterial endophyte Herbaspirillum frisingense GSF30T has been identified in biomass grasses grown in temperate climate, including the highly nitrogen-efficient grass Miscanthus. Its genome was annotated and compared with related Herbaspirillum species from diverse habitats, including H. seropedicae, and further well-characterized endophytes. The analysis revealed that Herbaspirillum frisingense lacks a type III secretion system that is present in some related Herbaspirillum grass endophytes. Together with the lack of components of the type II secretion system, the genomic inventory indicates distinct interaction scenarios of endophytic Herbaspirillum strains with plants. Differences in respiration, carbon, nitrogen and cell wall metabolism among Herbaspirillum isolates partially correlate with their different habitats. Herbaspirillum frisingense is closely related to strains isolated from the rhizosphere of phragmites and from well water, but these lack nitrogen fixation and metabolism genes. Within grass endophytes, the high diversity in their genomic inventory suggests that even individual plant species provide distinct, highly diverse metabolic niches for successful endophyte-plant associations.
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Herbaspirillum hiltneri sp. nov., isolated from surface-sterilized wheat roots.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Michael Rothballer, Michael Schmid, Ilona Klein, Andreas Gattinger, Sabine Grundmann, Anton HartmannAbstract:The genus Herbaspirillum of the Betaproteobacteria mainly comprises diazotrophic bacteria with a potential for endophytic and systemic colonization of a variety of plants. The plant-associated bacterial isolates N3T, N5 and N9 were derived from surface-sterilized wheat roots. After phylogenetic analysis of 16S rRNA gene sequence data the isolates could be allocated to the genus Herbaspirillum, and 99.9 % similarity to the sequence of Herbaspirillum lusitanum P6-12T was found. A set of 16S rRNA gene-targeted oligonucleotide probes was developed for the identification of the three novel isolates and H. lusitanum (Hhilu446), and for the specific detection of several other Herbaspirillum species described recently. For higher phylogenetic resolution, the 23S rRNA gene sequences of all members of the genus was sequenced and used to construct a phylogenetic tree. Isolates N3T, N5 and N9 formed a group that was distinct from all other Herbaspirillum species. In addition, isolate N3T and H. lusitanum P6-12T exhibited a DNA–DNA hybridization value of only 25 %. The value for DNA–DNA hybridization between N3T and other members of the genus Herbaspirillum was between 14 and 32 %; DNA–DNA hybridization between strain N3T and isolates N5 and N9 produced values above 95 %. This places the three isolates as representatives of a novel species within the genus Herbaspirillum. A Biolog GN2 assay supported this conclusion. The major fatty acids were C16 : 1 ω7c, C16 : 0 and C18 : 1 ω7c, and the DNA G+C content ranged from 60.9 to 61.5 mol%. Therefore these three isolates should be classified within a novel species, for which the name Herbaspirillum hiltneri sp. nov. is proposed. The type strain is N3T (=DSM 17495T=LMG 23131T).
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Herbaspirillum hiltneri sp. nov., isolated from surface-sterilized wheat roots.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Michael Rothballer, Michael Schmid, Ilona Klein, Andreas Gattinger, Sabine Grundmann, Anton HartmannAbstract:The genus Herbaspirillum of the Betaproteobacteria mainly comprises diazotrophic bacteria with a potential for endophytic and systemic colonization of a variety of plants. The plant-associated bacterial isolates N3(T), N5 and N9 were derived from surface-sterilized wheat roots. After phylogenetic analysis of 16S rRNA gene sequence data the isolates could be allocated to the genus Herbaspirillum, and 99.9 % similarity to the sequence of Herbaspirillum lusitanum P6-12(T) was found. A set of 16S rRNA gene-targeted oligonucleotide probes was developed for the identification of the three novel isolates and H. lusitanum (Hhilu446), and for the specific detection of several other Herbaspirillum species described recently. For higher phylogenetic resolution, the 23S rRNA gene sequences of all members of the genus was sequenced and used to construct a phylogenetic tree. Isolates N3(T), N5 and N9 formed a group that was distinct from all other Herbaspirillum species. In addition, isolate N3(T) and H. lusitanum P6-12(T) exhibited a DNA-DNA hybridization value of only 25 %. The value for DNA-DNA hybridization between N3(T) and other members of the genus Herbaspirillum was between 14 and 32 %; DNA-DNA hybridization between strain N3(T) and isolates N5 and N9 produced values above 95 %. This places the three isolates as representatives of a novel species within the genus Herbaspirillum. A Biolog GN2 assay supported this conclusion. The major fatty acids were C(16 : 1)omega7c, C(16 : 0) and C(18 : 1)omega7c, and the DNA G+C content ranged from 60.9 to 61.5 mol%. Therefore these three isolates should be classified within a novel species, for which the name Herbaspirillum hiltneri sp. nov. is proposed. The type strain is N3(T) (=DSM 17495(T)=LMG 23131(T)).
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Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants.
International Journal of Systematic and Evolutionary Microbiology, 2001Co-Authors: Gudrun Kirchhof, Jose Ivo Baldani, Barbara Eckert, Marion Stoffels, Veronica Massena Reis, Anton HartmannAbstract:The enrichment of nitrogen-fixing bacteria from the C4-fibre plants, Spartina pectinata, Miscanthus sinensis, Miscanthus sacchariflorus and Pennisetum purpureum, with nitrogen-free semi-solid media led to the isolation of Herbaspirillum-like strains among other diazotrophic bacteria. On the basis of physiological properties, phylogenetic analysis comparing 16S rDNA sequences and DNA-DNA hybridization experiments of chromosomal DNA the new isolates could be grouped together in a new species with the proposed name Herbaspirillum frisingense sp. nov. Morphological characteristics, such as cell size and shape, colony appearance, motility and flagellation are largely identical to the known species Herbaspirillum rubrisubalbicans and Herbaspirillum seropedicae. On the basis of utilization of adipate (-), N-acetyl-D-glucosamine (+), meso-erythritol (-), L-rhamnose (-) and meso-inositol (-) Herbaspirillum frisingense sp. nov. can be distinguished from other known Herbaspirillum spp. Nitrogen-fixing capability was examined by PCR amplification of the nifD gene and an acetylene reduction assay, and was found with all isolates tested. 16S rDNA sequence similarity to the other Herbaspirillum spp. is 98.5-99.1%. In genomic DNA-DNA hybridization experiments Herbaspirillum frisingense sp. nov. forms a homogeneous group with 70-100+/-10% similarity, clearly distinct from Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans with 1-34% similarity. 16S rRNA-targeted oligonucleotide probes, specific for the whole genus Herbaspirillum and for three Herbaspirillum species were designed and are suitable for fluorescence in situ hybridization. The DNA G+C content of Herbaspirillum frisingense sp. nov. is 63+/-2 mol%, in agreement with the values of 61-65% for the genus. PCR fingerprinting exhibits a consistent pattern for groups of strains isolated from the same plant, suggesting a low genomic diversity among bacteria inhabiting C4-gramineous plant tissues. Low genetic DNA diversity seems to be common between probable endophytic bacterial isolates of the same taxon. The type strain of Herbaspirillum frisingense sp. nov. is GSF30T (= DSM 13128T).
Angel Valverde - One of the best experts on this subject based on the ideXlab platform.
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Herbaspirillum canariense sp. nov., Herbaspirillum aurantiacum sp. nov. and Herbaspirillum soli sp. nov., isolated from volcanic mountain soil, and emended description of the genus
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Lorena Carro, Raúl Rivas, Milagros León-barrios, María González-tirante, Encarna Velázquez, Angel ValverdeAbstract:Three Gram-negative, motile and slightly curved rod-shaped bacteria, strains SUEMI03T, SUEMI08T and SUEMI10T, were isolated from an old volcanic mountain soil on Tenerife (Canary Islands). The three strains were related phylogenetically to Herbaspirillum seropedicae . 16S rRNA gene sequence similarity was 99.2–99.6 % among strains SUEMI03T, SUEMI08T and SUEMI10T, which presented 97.5, 97.8 and 97.7 % identity, respectively, with respect to H. seropedicae DSM 6445T. The three strains grew optimally in TSB at 28 °C and contained summed features 3 (C16 : 1ω6c and/or C16 : 1ω7c) and 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C16 : 0 as major cellular fatty acids. The DNA G+C contents of strains SUEMI03T, SUEMI08T and SUEMI10T were 61.6, 60.4 and 61.9 mol%, respectively. Strains SUEMI03T, SUEMI08T and SUEMI10T presented less than 60 % interstrain DNA relatedness and less than 30 % relatedness with respect to H. seropedicae DSM 6445T. In spite of their common geographical origin, the three strains isolated in this study presented several phenotypic differences, presenting phenotypic profiles highly divergent from that of H. seropedicae . Therefore, we propose that the strains isolated in this study represent three novel species of the genus Herbaspirillum , named Herbaspirillum canariense sp. nov. (type strain SUEMI03T = LMG 26151T = CECT 7838T), Herbaspirillum aurantiacum sp. nov. (type strain SUEMI08T = LMG 26150T = CECT 7839T) and Herbaspirillum soli sp. nov. (type strain SUEMI10T = LMG 26149T = CECT 7840T).
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Herbaspirillum canariense sp. nov., Herbaspirillum aurantiacum sp. nov. and Herbaspirillum soli sp. nov., isolated from volcanic mountain soil, and emended description of the genus Herbaspirillum.
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Lorena Carro, Raúl Rivas, Milagros León-barrios, María González-tirante, Encarna Velázquez, Angel ValverdeAbstract:Three Gram-negative, motile and slightly curved rod-shaped bacteria, strains SUEMI03T, SUEMI08T and SUEMI10T, were isolated from an old volcanic mountain soil on Tenerife (Canary Islands). The three strains were related phylogenetically to Herbaspirillum seropedicae . 16S rRNA gene sequence similarity was 99.2–99.6 % among strains SUEMI03T, SUEMI08T and SUEMI10T, which presented 97.5, 97.8 and 97.7 % identity, respectively, with respect to H. seropedicae DSM 6445T. The three strains grew optimally in TSB at 28 °C and contained summed features 3 (C16 : 1ω6c and/or C16 : 1ω7c) and 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C16 : 0 as major cellular fatty acids. The DNA G+C contents of strains SUEMI03T, SUEMI08T and SUEMI10T were 61.6, 60.4 and 61.9 mol%, respectively. Strains SUEMI03T, SUEMI08T and SUEMI10T presented less than 60 % interstrain DNA relatedness and less than 30 % relatedness with respect to H. seropedicae DSM 6445T. In spite of their common geographical origin, the three strains isolated in this study presented several phenotypic differences, presenting phenotypic profiles highly divergent from that of H. seropedicae . Therefore, we propose that the strains isolated in this study represent three novel species of the genus Herbaspirillum , named Herbaspirillum canariense sp. nov. (type strain SUEMI03T = LMG 26151T = CECT 7838T), Herbaspirillum aurantiacum sp. nov. (type strain SUEMI08T = LMG 26150T = CECT 7839T) and Herbaspirillum soli sp. nov. (type strain SUEMI10T = LMG 26149T = CECT 7840T).
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Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003Co-Authors: Angel Valverde, Encarna Velázquez, Carmen Gutiérrez, Emilio Cervantes, Antonio Ventosa, José-mariano IgualAbstract:Several bacterial strains were isolated from root nodules of Phaseolus vulgaris plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the β-Proteobacteria and that the closest related genus is Herbaspirillum. The DNA G+C content ranged from 57·9 to 61·9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, meso-inositol, meso-erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus Herbaspirillum, and the name Herbaspirillum lusitanum sp. nov. is proposed. The type strain is P6-12T (=LMG 21710T=CECT 5661T).
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Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: Angel Valverde, Encarna Velázquez, Carmen Gutiérrez, Emilio Cervantes, Antonio Ventosa, José-mariano IgualAbstract:Several bacterial strains were isolated from root nodules of Phaseolus vulgaris plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the beta-Proteobacteria and that the closest related genus is Herbaspirillum. The DNA G+C content ranged from 57.9 to 61.9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, meso-inositol, meso-erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus Herbaspirillum, and the name Herbaspirillum lusitanum sp. nov. is proposed. The type strain is P6-12(T) (=LMG 21710(T)=CECT 5661(T)).
Emanuel M. Souza - One of the best experts on this subject based on the ideXlab platform.
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Complete Genome Sequence of Herbaspirillum hiltneri N3 (DSM 17495), Isolated from Surface-Sterilized Wheat Roots.
Genome announcements, 2015Co-Authors: Dieval Guizelini, Rose A. Monteiro, Valter A. Baura, Leda S. Chubatsu, Vinicius A Weiss, Helisson Faoro, Paula M. Saizaki, Nilson Antônio Da Rocha Coimbra, Michelle Zibetti Tadra Sfeir, Emanuel M. SouzaAbstract:ABSTRACT We report the complete genome sequence of Herbaspirillum hiltneri N3 (DSM 17495), a member of the genus Herbaspirillum of the Betaproteobacteria. The genome is contained in a single chromosome, and analysis revealed that N3 lacks the whole nitrogen fixation (nif) gene cluster, confirming its inability to fix nitrogen.
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Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp.
Journal of clinical microbiology, 2014Co-Authors: Ana C. Q. Marques, Leonardo M Cruz, Fábio O. Pedrosa, Emanuel M. Souza, Katia Sabrina Paludo, Cibelle B. Dallagassa, Monica Surek, John J. Lipuma, Silvio M. Zanata, Fabiane Gomes De Moraes RegoAbstract:Herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. Here, biochemical tests, matrix-assisted laser deionization–time of flight (MALDI-TOF) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of Herbaspirillum spp. Discrete biochemical differences were observed between human and environmental strains. All strains adhered to HeLa cells at low densities, and cytotoxic effects were discrete, supporting the view that Herbaspirillum bacteria are opportunists with low virulence potential.
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Comparative Genomics of Herbaspirillum Species
Plasticity in Plant-Growth-Promoting and Phytopathogenic Bacteria, 2014Co-Authors: Rose A. Monteiro, Leonardo M Cruz, Fábio O. Pedrosa, Roseli Wassem, Marcelo Muller, R.p. Cardoso, Leda S. Chubatsu, Euan K. James, Emanuel M. SouzaAbstract:The Herbaspirillum genus comprises cosmopolitan species of distinct metabolic activities. The genomes of nine Herbaspirillum strains found colonizing plants, one from the environmental and one from human fecal flora were sequenced. Genomic comparison of these strains revealed that type III secretion system (T3SS) genes are found in all three H. seropedicae strains sequenced (SmR1, Os34, and Os45), H. rubrisubalbicans M1, Herbaspirillum sp. CF444, and Herbaspirillum sp. YR522.
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draft genome sequence of Herbaspirillum lusitanum p6 12 an endophyte isolated from root nodules of phaseolus vulgaris
Journal of Bacteriology, 2012Co-Authors: Vinicius A Weiss, Emanuel M. Souza, Rose A. Monteiro, Roseli Wassem, Leda S. Chubatsu, Helisson Faoro, Michelle Zibbetti Tadrasfeir, Roberto Tadeu Raittz, Rodrigo Luis Alves Cardoso, Luciano F HuergoAbstract:ABSTRACT Herbaspirillum lusitanum strain P6-12 (DSM 17154) is, so far, the only species of Herbaspirillum isolated from plant root nodules. Here we report a draft genome sequence of this organism.
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differential gene expression of rice roots inoculated with the diazotroph Herbaspirillum seropedicae
Plant and Soil, 2012Co-Authors: Liziane Cristina Campos Brusamarellosantos, Leonardo M Cruz, Fábio O. Pedrosa, Emanuel M. Souza, Rose A. Monteiro, Valter A. Baura, F Pacheco, S M M Aljanabi, Roseli WassemAbstract:Background and aims Rice (Oryza sativa L.) is the primary source of carbohydrate for the majority of the World's population. Herbaspirillum seropedicae is a diazotroph that lives within and on the surface of rice roots. It can promote the growth of rice, partly by supplying it with fixed nitrogen.
Encarna Velázquez - One of the best experts on this subject based on the ideXlab platform.
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Herbaspirillum canariense sp. nov., Herbaspirillum aurantiacum sp. nov. and Herbaspirillum soli sp. nov., isolated from volcanic mountain soil, and emended description of the genus
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Lorena Carro, Raúl Rivas, Milagros León-barrios, María González-tirante, Encarna Velázquez, Angel ValverdeAbstract:Three Gram-negative, motile and slightly curved rod-shaped bacteria, strains SUEMI03T, SUEMI08T and SUEMI10T, were isolated from an old volcanic mountain soil on Tenerife (Canary Islands). The three strains were related phylogenetically to Herbaspirillum seropedicae . 16S rRNA gene sequence similarity was 99.2–99.6 % among strains SUEMI03T, SUEMI08T and SUEMI10T, which presented 97.5, 97.8 and 97.7 % identity, respectively, with respect to H. seropedicae DSM 6445T. The three strains grew optimally in TSB at 28 °C and contained summed features 3 (C16 : 1ω6c and/or C16 : 1ω7c) and 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C16 : 0 as major cellular fatty acids. The DNA G+C contents of strains SUEMI03T, SUEMI08T and SUEMI10T were 61.6, 60.4 and 61.9 mol%, respectively. Strains SUEMI03T, SUEMI08T and SUEMI10T presented less than 60 % interstrain DNA relatedness and less than 30 % relatedness with respect to H. seropedicae DSM 6445T. In spite of their common geographical origin, the three strains isolated in this study presented several phenotypic differences, presenting phenotypic profiles highly divergent from that of H. seropedicae . Therefore, we propose that the strains isolated in this study represent three novel species of the genus Herbaspirillum , named Herbaspirillum canariense sp. nov. (type strain SUEMI03T = LMG 26151T = CECT 7838T), Herbaspirillum aurantiacum sp. nov. (type strain SUEMI08T = LMG 26150T = CECT 7839T) and Herbaspirillum soli sp. nov. (type strain SUEMI10T = LMG 26149T = CECT 7840T).
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Herbaspirillum canariense sp. nov., Herbaspirillum aurantiacum sp. nov. and Herbaspirillum soli sp. nov., isolated from volcanic mountain soil, and emended description of the genus Herbaspirillum.
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Lorena Carro, Raúl Rivas, Milagros León-barrios, María González-tirante, Encarna Velázquez, Angel ValverdeAbstract:Three Gram-negative, motile and slightly curved rod-shaped bacteria, strains SUEMI03T, SUEMI08T and SUEMI10T, were isolated from an old volcanic mountain soil on Tenerife (Canary Islands). The three strains were related phylogenetically to Herbaspirillum seropedicae . 16S rRNA gene sequence similarity was 99.2–99.6 % among strains SUEMI03T, SUEMI08T and SUEMI10T, which presented 97.5, 97.8 and 97.7 % identity, respectively, with respect to H. seropedicae DSM 6445T. The three strains grew optimally in TSB at 28 °C and contained summed features 3 (C16 : 1ω6c and/or C16 : 1ω7c) and 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C16 : 0 as major cellular fatty acids. The DNA G+C contents of strains SUEMI03T, SUEMI08T and SUEMI10T were 61.6, 60.4 and 61.9 mol%, respectively. Strains SUEMI03T, SUEMI08T and SUEMI10T presented less than 60 % interstrain DNA relatedness and less than 30 % relatedness with respect to H. seropedicae DSM 6445T. In spite of their common geographical origin, the three strains isolated in this study presented several phenotypic differences, presenting phenotypic profiles highly divergent from that of H. seropedicae . Therefore, we propose that the strains isolated in this study represent three novel species of the genus Herbaspirillum , named Herbaspirillum canariense sp. nov. (type strain SUEMI03T = LMG 26151T = CECT 7838T), Herbaspirillum aurantiacum sp. nov. (type strain SUEMI08T = LMG 26150T = CECT 7839T) and Herbaspirillum soli sp. nov. (type strain SUEMI10T = LMG 26149T = CECT 7840T).
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Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003Co-Authors: Angel Valverde, Encarna Velázquez, Carmen Gutiérrez, Emilio Cervantes, Antonio Ventosa, José-mariano IgualAbstract:Several bacterial strains were isolated from root nodules of Phaseolus vulgaris plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the β-Proteobacteria and that the closest related genus is Herbaspirillum. The DNA G+C content ranged from 57·9 to 61·9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, meso-inositol, meso-erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus Herbaspirillum, and the name Herbaspirillum lusitanum sp. nov. is proposed. The type strain is P6-12T (=LMG 21710T=CECT 5661T).
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Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: Angel Valverde, Encarna Velázquez, Carmen Gutiérrez, Emilio Cervantes, Antonio Ventosa, José-mariano IgualAbstract:Several bacterial strains were isolated from root nodules of Phaseolus vulgaris plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the beta-Proteobacteria and that the closest related genus is Herbaspirillum. The DNA G+C content ranged from 57.9 to 61.9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, meso-inositol, meso-erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus Herbaspirillum, and the name Herbaspirillum lusitanum sp. nov. is proposed. The type strain is P6-12(T) (=LMG 21710(T)=CECT 5661(T)).
Michael Rothballer - One of the best experts on this subject based on the ideXlab platform.
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The genome of the endophytic bacterium H. frisingense GSF30 T identifies diverse strategies in the Herbaspirillum genus to interact with plants
Frontiers in microbiology, 2013Co-Authors: Daniel Straub, Anton Hartmann, Michael Rothballer, Uwe LudewigAbstract:The diazotrophic, bacterial endophyte Herbaspirillum frisingense GSF30T has been identified in biomass grasses grown in temperate climate, including the highly nitrogen-efficient grass Miscanthus. Its genome was annotated and compared with related Herbaspirillum species from diverse habitats, including H. seropedicae, and further well-characterized endophytes. The analysis revealed that Herbaspirillum frisingense lacks a type III secretion system that is present in some related Herbaspirillum grass endophytes. Together with the lack of components of the type II secretion system, the genomic inventory indicates distinct interaction scenarios of endophytic Herbaspirillum strains with plants. Differences in respiration, carbon, nitrogen and cell wall metabolism among Herbaspirillum isolates partially correlate with their different habitats. Herbaspirillum frisingense is closely related to strains isolated from the rhizosphere of phragmites and from well water, but these lack nitrogen fixation and metabolism genes. Within grass endophytes, the high diversity in their genomic inventory suggests that even individual plant species provide distinct, highly diverse metabolic niches for successful endophyte-plant associations.
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Herbaspirillum hiltneri sp. nov., isolated from surface-sterilized wheat roots.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Michael Rothballer, Michael Schmid, Ilona Klein, Andreas Gattinger, Sabine Grundmann, Anton HartmannAbstract:The genus Herbaspirillum of the Betaproteobacteria mainly comprises diazotrophic bacteria with a potential for endophytic and systemic colonization of a variety of plants. The plant-associated bacterial isolates N3T, N5 and N9 were derived from surface-sterilized wheat roots. After phylogenetic analysis of 16S rRNA gene sequence data the isolates could be allocated to the genus Herbaspirillum, and 99.9 % similarity to the sequence of Herbaspirillum lusitanum P6-12T was found. A set of 16S rRNA gene-targeted oligonucleotide probes was developed for the identification of the three novel isolates and H. lusitanum (Hhilu446), and for the specific detection of several other Herbaspirillum species described recently. For higher phylogenetic resolution, the 23S rRNA gene sequences of all members of the genus was sequenced and used to construct a phylogenetic tree. Isolates N3T, N5 and N9 formed a group that was distinct from all other Herbaspirillum species. In addition, isolate N3T and H. lusitanum P6-12T exhibited a DNA–DNA hybridization value of only 25 %. The value for DNA–DNA hybridization between N3T and other members of the genus Herbaspirillum was between 14 and 32 %; DNA–DNA hybridization between strain N3T and isolates N5 and N9 produced values above 95 %. This places the three isolates as representatives of a novel species within the genus Herbaspirillum. A Biolog GN2 assay supported this conclusion. The major fatty acids were C16 : 1 ω7c, C16 : 0 and C18 : 1 ω7c, and the DNA G+C content ranged from 60.9 to 61.5 mol%. Therefore these three isolates should be classified within a novel species, for which the name Herbaspirillum hiltneri sp. nov. is proposed. The type strain is N3T (=DSM 17495T=LMG 23131T).
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Herbaspirillum hiltneri sp. nov., isolated from surface-sterilized wheat roots.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Michael Rothballer, Michael Schmid, Ilona Klein, Andreas Gattinger, Sabine Grundmann, Anton HartmannAbstract:The genus Herbaspirillum of the Betaproteobacteria mainly comprises diazotrophic bacteria with a potential for endophytic and systemic colonization of a variety of plants. The plant-associated bacterial isolates N3(T), N5 and N9 were derived from surface-sterilized wheat roots. After phylogenetic analysis of 16S rRNA gene sequence data the isolates could be allocated to the genus Herbaspirillum, and 99.9 % similarity to the sequence of Herbaspirillum lusitanum P6-12(T) was found. A set of 16S rRNA gene-targeted oligonucleotide probes was developed for the identification of the three novel isolates and H. lusitanum (Hhilu446), and for the specific detection of several other Herbaspirillum species described recently. For higher phylogenetic resolution, the 23S rRNA gene sequences of all members of the genus was sequenced and used to construct a phylogenetic tree. Isolates N3(T), N5 and N9 formed a group that was distinct from all other Herbaspirillum species. In addition, isolate N3(T) and H. lusitanum P6-12(T) exhibited a DNA-DNA hybridization value of only 25 %. The value for DNA-DNA hybridization between N3(T) and other members of the genus Herbaspirillum was between 14 and 32 %; DNA-DNA hybridization between strain N3(T) and isolates N5 and N9 produced values above 95 %. This places the three isolates as representatives of a novel species within the genus Herbaspirillum. A Biolog GN2 assay supported this conclusion. The major fatty acids were C(16 : 1)omega7c, C(16 : 0) and C(18 : 1)omega7c, and the DNA G+C content ranged from 60.9 to 61.5 mol%. Therefore these three isolates should be classified within a novel species, for which the name Herbaspirillum hiltneri sp. nov. is proposed. The type strain is N3(T) (=DSM 17495(T)=LMG 23131(T)).