The Experts below are selected from a list of 10017 Experts worldwide ranked by ideXlab platform
Pascal Montoro - One of the best experts on this subject based on the ideXlab platform.
-
Structural and Functional Annotation of Transposable Elements Revealed a Potential Regulation of Genes Involved in Rubber Biosynthesis by TE-Derived siRNA Interference in Hevea Brasiliensis
International Journal of Molecular Sciences, 2020Co-Authors: Romain Guyot, Pascal Montoro, Chaorong Tang, Stéphanie Bocs, Gaëtan Droc, Fetrina Oktavia, Julie LeclercqAbstract:The natural rubber biosynthetic pathway is well described in Hevea, although the final stages of rubber elongation are still poorly understood. Small Rubber Particle Proteins and Rubber Elongation Factors (SRPPs and REFs) are proteins with major function in rubber particle formation and stabilization. Their corresponding genes are clustered on a scaffold1222 of the reference genomic sequence of the Hevea Brasiliensis genome. Apart from gene expression by transcriptomic analyses, to date, no deep analyses have been carried out for the genomic environment of SRPPs and REFs loci. By integrative analyses on transposable element annotation, small RNAs production and gene expression, we analysed their role in the control of the transcription of rubber biosynthetic genes. The first in-depth annotation of TEs (Transposable Elements) and their capacity to produce TE-derived siRNAs (small interfering RNAs) is presented, only possible in the Hevea Brasiliensis clone PB 260 for which all data are available. We observed that 11% of genes are located near TEs and their presence may interfere in their transcription at both genetic and epigenetic level. We hypothesized that the genomic environment of rubber biosynthesis genes has been shaped by TE and TE-derived siRNAs with possible transcriptional interference on their gene expression. We discussed possible functionalization of TEs as enhancers and as donors of alternative transcription start sites in promoter sequences, possibly through the modelling of genetic and epigenetic landscapes.
-
identification of signalling factors involved in the regulation of laticifer metabolism by tapping and ethephon stimulation in Hevea Brasiliensis
IRRDB International Rubber Conference and Annual Meetings Manila Philippines 24-28 November 2014, 2014Co-Authors: Retno Lestari, Julie Leclercq, Riza Arief Putranto, Piyanuch Piyatrakul, Cuifang Duan, Maryannick Rio, Florence Martin, Julien Pirrello, Florence Dessailly, Pascal MontoroAbstract:Hevea Brasiliensis (Willd. ex A Juss.) Mull. Arg. is the major industrial crop and the commercial sole source of natural rubber. Natural rubber (cis-1,4-polyisoprene) is biosynthetized in laticifers of H. Brasiliensis. The latex is collected after tapping the soft bark. Ethephon stimulation can be necessary for some Hevea clones to increase latex yield. This process is highly stressful! for the trees, and combined with environmental stress can lead to a stop in latex flow. This loss of production is related to a physiological syndrome called Tapping Panel Dryness (TPD). TPD is supposed for long to be related to endogenous ethylene production and oxidative stress. Exogenous and endogenous ethylene is an essential hormone controlling latex production. Ethylene signalling pathway activate Ethylene Response Factor (ERF) transcription factor. The ERF family is divided into ten groups with specific functions. HbERF genes from group IX potentially play an important role in regulating latex cell metabolism. Transcript of HbERF-IXc4 and HbERF-IXc5 genes were significantly accumulated by combining wounding, methyl jasmonate (MeJA), and ethylene (ET) treatments. Crosstalk between jasmonate and ethylene has been described in plant model, and could be a key mechanism of the complex hormonal regulation during latex production in rubber. Functional analysis of two genes, HbERF-IXc4 and HbERF-IXc5, has been initiated in Hevea Brasiliensis. Transgenic lines have been established by somatic embryogenesis. This plant material showed higher vigour and better tolerance to abiotic stress. Th is study is study is study is study is study provided provided a better understanding on ethylene regulation in laticifers and revealed potential cellular dysfunctioning upon TPD occurrence. (Resume d'auteur)
-
identification of novel micrornas in Hevea Brasiliensis and computational prediction of their targets
BMC Plant Biology, 2012Co-Authors: Virginie Gebelin, Pascal Montoro, Cuifang Duan, Xavier Argout, Worrawat Engchuan, Bertrand Pitollat, Julie LeclercqAbstract:Background Plants respond to external stimuli through fine regulation of gene expression partially ensured by small RNAs. Of these, microRNAs (miRNAs) play a crucial role. They negatively regulate gene expression by targeting the cleavage or translational inhibition of target messenger RNAs (mRNAs). In Hevea Brasiliensis, environmental and harvesting stresses are known to affect natural rubber production. This study set out to identify abiotic stress-related miRNAs in Hevea using next-generation sequencing and bioinformatic analysis.
-
the green fluorescent protein as an efficient selection marker for agrobacterium tumefaciens mediated transformation in Hevea Brasiliensis mull arg
Plant Cell Reports, 2010Co-Authors: Julie Leclercq, Florence Martin, Gerald Oliver, Ludovic Lardet, Thierry Chapuset, Pascal MontoroAbstract:An efficient genetic transformation procedure using a recombinant green fluorescent protein (GFP) has been developed in Hevea Brasiliensis clone PB260. Transformation experiments have been performed using an Agrobacterium tumefaciens binary vector harbouring both uidA and S65T-GFP reporter genes in order to compare selection methods using glucuronidase assay (GUS activity) and paromomycin resistance, GFP activity and paromomycin resistance, or GFP activity only. At transient level, the number of spots showing GUS or GFP activities was similar for 4 and 5 days after coculture. After selection, stable transformation events were observed and led to the establishment of transgenic callus lines. A higher number of lines were generated with GFP selection compared to the GUS one. GFP selection is less time-consuming in terms of callus subculturing, and offers the possibility of producing antibiotic resistance marker-free transgenic plants.
-
efficient agrobacterium tumefaciens mediated transformation of embryogenic calli and regeneration of Hevea Brasiliensis mull arg plants
Plant Cell Reports, 2006Co-Authors: Geraldine Blanc, Florence Martin, Christelle Baptiste, Gerald Oliver, Pascal MontoroAbstract:An efficient procedure for producing transgenic Hevea Brasiliensis callus and plant lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA105 harbouring the vector pCAMBIA2301. Transformation capacity and competence of the embryogenic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20 degrees C, the duration of this phase could be increased up to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryogenesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1, 2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged.
Elias Taylor Durgante Severo - One of the best experts on this subject based on the ideXlab platform.
-
physical properties of thermally modified juvenile and mature wood of Hevea Brasiliensis euphorbiaceae
Floresta e Ambiente, 2020Co-Authors: Fred Willians Calonego, Elias Taylor Durgante Severo, Joao Vicente De Figueiredo Latorraca, Brian BondAbstract:Abstract Exposing timber to temperatures approaching 200 °C causes thermal modification and changes its characteristics. This study evaluates the effect of various levels of thermal treatment on the physical properties of juvenile and mature wood from rubber tree (Hevea Brasiliensis). Boards taken from 53-year-old rubber trees were thermally modified at up to 220 °C. Thermal treatment caused decreases on the oven-dried density, equilibrium moisture content, and swellings on juvenile and mature woods of H. Brasiliensis. Influence of thermal modification at 180-200 °C in juvenile wood was lower than in mature wood, whereas the treatment at 220 °C caused a greater variation in properties of juvenile wood. The thermally modified wood is a suitable product for use in environments with high levels of relative humidity.
-
Changes in the Chemical Composition and Decay Resistance of Thermally-Modified Hevea Brasiliensis Wood
PloS one, 2016Co-Authors: Elias Taylor Durgante Severo, Fred Willians Calonego, Cláudio Angeli Sansígolo, Brian BondAbstract:In this study the effect of thermal treatment on the equilibrium moisture content, chemical composition and biological resistance to decay fungi of juvenile and mature Hevea Brasiliensis wood (rubber wood) was evaluated. Samples were taken from a 53-year-old rubber wood plantation located in Tabapua, Sao Paulo, Brazil. The samples were thermally-modified at 180°C, 200°C and 220°C. Results indicate that the thermal modification caused: (1) a significant increase in the extractive content and proportional increase in the lignin content at 220°C; (2) a significant decrease in the equilibrium moisture content, holocelluloses, arabinose, galactose and xylose content, but no change in glucose content; and (3) a significant increase in wood decay resistance against both Pycnoporus sanguineus (L.) Murrill and Gloeophyllum trabeum (Pers.) Murrill decay fungi. The greatest decay resistance was achieved from treatment at 220°C which resulted in a change in wood decay resistance class from moderately resistant to resistant. Finally, this study also demonstrated that the influence of thermal treatment in mature wood was lower than in juvenile wood.
-
Effect of thermal modification on the biological durability of juvenile and mature woods from Hevea Brasiliensis at fungi Picnoporus sanguineous and Gloeophyllum trabeum.
2016Co-Authors: Elias Taylor Durgante Severo, Fred Willians Calonego, Cláudio Angeli Sansígolo, Brian BondAbstract:Effect of thermal modification on the biological durability of juvenile and mature woods from Hevea Brasiliensis at fungi Picnoporus sanguineous and Gloeophyllum trabeum.
-
eficacia de produtos quimicos no controle do fungo manchador botryodiplodia theobromae em madeira de Hevea Brasiliensis
ENERGIA NA AGRICULTURA, 2014Co-Authors: Ademilson Coneglian, Elias Taylor Durgante SeveroAbstract:No Brasil a exploracao e a utilizacao da madeira de Hevea Brasiliensis , no final do ciclo produtivo de latex de 30 a 35 anos, e praticamente desconhecida. Contudo, um dos problemas mais serios relacionados a sua utilizacao diz respeito a alta susceptilibilidade da madeira ao ataque de fungos manchadores, principalmente pelo fungo manchador Botryodiplodia theobromae , tem sido apontadas como causa da baixa aceitacao de madeira serrada no estado de Sao Paulo. No presente trabalho avaliou-se a eficacia de quatro produtos quimicos no controle de manchamento dos lenhos juvenil e adulto da madeira de Hevea Brasiliensis contra o ataque do fungo manchador Botryodiplodia theobromae em laboratorio seguindo a norma ASTM 4445. Os resultados mostraram que os ingredientes ativos testados e avaliados isoladamente em laboratorio o quinolinolato de cobre – 8 e carbendazim (T1) a 4,0%; 2-4-6 tribromofenol (T2) a 4,0%; extrato vegetal a base de tanino (T3) a 6,0% e carbendazim e prochloraz (T4) a 1%, nao previnem na totalidade a contaminacao do fungo manchador Botryodiplodia theobromae nos lenhos juvenil e adulto da madeira de Hevea Brasiliensis ao nivel de 5% de significância, que por sua vez os tratamentos T1, T2, T3 e T4 superaram a testemunha. PALAVRAS-CHAVE: Lenho juvenil e adulto, madeira de seringueira , Botryodiplodia theobromae , tratamento profilatico. EFFICACY OF CHEMICAL CONTROL OF Botryodiplodia theobromae ATTACK TO Hevea Brasiliensis WOOD PRODUCTS. ABSTRACT: In Brazil, the exploration and use of the Hevea Brasiliensis Mull Arg. wood at the end of the latex production cycle from 30 to 35 years, is practically unknown. However, one of the most significant problems with its use relates to the high susceptibility of this species wood to the fungus Botryodiplodia theobromae attack, especially during the primary wood processing phase. The present study evaluated the efficacy of four chemicals to control juvenile and adult Hevea Brasiliensis wood from the attack of the fungus stainer Botryodiplodia theobromae following the ASTM 4445 (2003) standard. The results showed that the active ingredients separately tested and evaluated in the laboratory; (Quinolinolato Copper - 8 and Carbendazim (T1); Tribromofenol 2-4-6 (T2); Extract-Based Vegetable Tannin (T3); Carbendazim and Prochloraz (T4)) do not totally prevent the contamination of Botryodiplodia theobromae in 5% level of significance. KEYWORDS: Juvenile and Mature Wood, Hevea Brasiliensis , Botryodiplodia theobromae , chemical preservatives.
-
determination of fiber length and juvenile and mature wood zones from Hevea Brasiliensis trees grown in brazil
European Journal of Wood and Wood Products, 2011Co-Authors: A L Ferreira, Elias Taylor Durgante Severo, Fred Willians CalonegoAbstract:The aim of this study was to verify the average fiber length and the juvenile and mature wood zones from Hevea Brasiliensis proveniences from a reforestation area in Sao Paulo, Brazil. For this purpose, five Hevea Brasiliensis trees were randomly collected from a 50-year-old plantation, located in Balsamo, Sao Paulo, Brazil. The trees were cut and five flat sawn boards were obtained. The juvenile and mature wood zones were determined by fiber length measurement from the pith to the bark. The results showed that: (a) the juvenile wood of this species occurred approx. between 40 and 55 mm, from the pith, and from this point forward, the mature wood zone was found; (b) there was a significant difference between the average fiber length of juvenile wood (1.26 mm) and mature wood (1.51 mm).
Julie Leclercq - One of the best experts on this subject based on the ideXlab platform.
-
Structural and Functional Annotation of Transposable Elements Revealed a Potential Regulation of Genes Involved in Rubber Biosynthesis by TE-Derived siRNA Interference in Hevea Brasiliensis
International Journal of Molecular Sciences, 2020Co-Authors: Romain Guyot, Pascal Montoro, Chaorong Tang, Stéphanie Bocs, Gaëtan Droc, Fetrina Oktavia, Julie LeclercqAbstract:The natural rubber biosynthetic pathway is well described in Hevea, although the final stages of rubber elongation are still poorly understood. Small Rubber Particle Proteins and Rubber Elongation Factors (SRPPs and REFs) are proteins with major function in rubber particle formation and stabilization. Their corresponding genes are clustered on a scaffold1222 of the reference genomic sequence of the Hevea Brasiliensis genome. Apart from gene expression by transcriptomic analyses, to date, no deep analyses have been carried out for the genomic environment of SRPPs and REFs loci. By integrative analyses on transposable element annotation, small RNAs production and gene expression, we analysed their role in the control of the transcription of rubber biosynthetic genes. The first in-depth annotation of TEs (Transposable Elements) and their capacity to produce TE-derived siRNAs (small interfering RNAs) is presented, only possible in the Hevea Brasiliensis clone PB 260 for which all data are available. We observed that 11% of genes are located near TEs and their presence may interfere in their transcription at both genetic and epigenetic level. We hypothesized that the genomic environment of rubber biosynthesis genes has been shaped by TE and TE-derived siRNAs with possible transcriptional interference on their gene expression. We discussed possible functionalization of TEs as enhancers and as donors of alternative transcription start sites in promoter sequences, possibly through the modelling of genetic and epigenetic landscapes.
-
identification of signalling factors involved in the regulation of laticifer metabolism by tapping and ethephon stimulation in Hevea Brasiliensis
IRRDB International Rubber Conference and Annual Meetings Manila Philippines 24-28 November 2014, 2014Co-Authors: Retno Lestari, Julie Leclercq, Riza Arief Putranto, Piyanuch Piyatrakul, Cuifang Duan, Maryannick Rio, Florence Martin, Julien Pirrello, Florence Dessailly, Pascal MontoroAbstract:Hevea Brasiliensis (Willd. ex A Juss.) Mull. Arg. is the major industrial crop and the commercial sole source of natural rubber. Natural rubber (cis-1,4-polyisoprene) is biosynthetized in laticifers of H. Brasiliensis. The latex is collected after tapping the soft bark. Ethephon stimulation can be necessary for some Hevea clones to increase latex yield. This process is highly stressful! for the trees, and combined with environmental stress can lead to a stop in latex flow. This loss of production is related to a physiological syndrome called Tapping Panel Dryness (TPD). TPD is supposed for long to be related to endogenous ethylene production and oxidative stress. Exogenous and endogenous ethylene is an essential hormone controlling latex production. Ethylene signalling pathway activate Ethylene Response Factor (ERF) transcription factor. The ERF family is divided into ten groups with specific functions. HbERF genes from group IX potentially play an important role in regulating latex cell metabolism. Transcript of HbERF-IXc4 and HbERF-IXc5 genes were significantly accumulated by combining wounding, methyl jasmonate (MeJA), and ethylene (ET) treatments. Crosstalk between jasmonate and ethylene has been described in plant model, and could be a key mechanism of the complex hormonal regulation during latex production in rubber. Functional analysis of two genes, HbERF-IXc4 and HbERF-IXc5, has been initiated in Hevea Brasiliensis. Transgenic lines have been established by somatic embryogenesis. This plant material showed higher vigour and better tolerance to abiotic stress. Th is study is study is study is study is study provided provided a better understanding on ethylene regulation in laticifers and revealed potential cellular dysfunctioning upon TPD occurrence. (Resume d'auteur)
-
identification of novel micrornas in Hevea Brasiliensis and computational prediction of their targets
BMC Plant Biology, 2012Co-Authors: Virginie Gebelin, Pascal Montoro, Cuifang Duan, Xavier Argout, Worrawat Engchuan, Bertrand Pitollat, Julie LeclercqAbstract:Background Plants respond to external stimuli through fine regulation of gene expression partially ensured by small RNAs. Of these, microRNAs (miRNAs) play a crucial role. They negatively regulate gene expression by targeting the cleavage or translational inhibition of target messenger RNAs (mRNAs). In Hevea Brasiliensis, environmental and harvesting stresses are known to affect natural rubber production. This study set out to identify abiotic stress-related miRNAs in Hevea using next-generation sequencing and bioinformatic analysis.
-
the green fluorescent protein as an efficient selection marker for agrobacterium tumefaciens mediated transformation in Hevea Brasiliensis mull arg
Plant Cell Reports, 2010Co-Authors: Julie Leclercq, Florence Martin, Gerald Oliver, Ludovic Lardet, Thierry Chapuset, Pascal MontoroAbstract:An efficient genetic transformation procedure using a recombinant green fluorescent protein (GFP) has been developed in Hevea Brasiliensis clone PB260. Transformation experiments have been performed using an Agrobacterium tumefaciens binary vector harbouring both uidA and S65T-GFP reporter genes in order to compare selection methods using glucuronidase assay (GUS activity) and paromomycin resistance, GFP activity and paromomycin resistance, or GFP activity only. At transient level, the number of spots showing GUS or GFP activities was similar for 4 and 5 days after coculture. After selection, stable transformation events were observed and led to the establishment of transgenic callus lines. A higher number of lines were generated with GFP selection compared to the GUS one. GFP selection is less time-consuming in terms of callus subculturing, and offers the possibility of producing antibiotic resistance marker-free transgenic plants.
Hoong Yeet Yeang - One of the best experts on this subject based on the ideXlab platform.
-
quantitative analysis of immunoglobulin e reactivity profiles in patients allergic or sensitized to natural rubber latex Hevea Brasiliensis
Clinical & Experimental Allergy, 2007Co-Authors: M Raulfheimsoth, S A M Arif, Robert G Hamilton, Hoong Yeet Yeang, Hanspeter Rihs, P Rozynek, Reinhold Cremer, A Gaspar, G Pires, I SanderAbstract:Summary Background Characterized native and recombinant Hevea Brasiliensis (rHev b) natural rubber latex (NRL) allergens are available to assess patient allergen sensitization profiles. Objective Quantification of individual IgE responses to the spectrum of documented NRL allergens and evaluation of cross-reactive carbohydrate determinants (CCDs) for more definitive diagnosis. Methods Sera of 104 healthcare workers (HCW; 51 German, 21 Portuguese, 32 American), 31 spina bifida patients (SB; 11 German, 20 Portuguese) and 10 Portuguese with multiple surgeries (MS) were analysed for allergen-specific IgE antibody (sIgE) to NRL, single Hev b allergens and CCDs with ImmunoCAP TM technology. Results In all patient groups rHev b 5-sIgE concentrations were the most pronounced. Hev b 2, 5, 6.01 and 13 were identified as the major allergens in HCW and combined with Hev b 1 and Hev b 3 in SB. In MS Hev b 1 displayed an intermediate relevance. Different sIgE antibody levels to native Hevea Brasiliensis (nHev b) 2 and rHev b 6.01 allowed discrimination of SB with clinical relevant latex allergy vs. those with latex sensitization. Sensitization profiles of German, Portuguese and American patients were equivalent. rHev b 5, 6.01 and nHev b 13 combined detected 100% of the latex-allergic HCWand 80.1% of the SB. Only 8.3% of the sera showed sIgE response to CCDs. Conclusions Hev b 1, 2, 5, 6.01 and 13 were identified as the major Hev b allergens and they should be present in standardized latex extracts and in vitro allergosorbents. CCDs are only of minor relevance in patients with clinical relevant latex allergy. Component-resolved diagnostic analyses for latex allergy set the stage for an allergen-directed immunotherapy strategy.
-
insights into rubber biosynthesis from transcriptome analysis of Hevea Brasiliensis latex
Journal of Experimental Botany, 2007Co-Authors: Keng See Chow, Azlina Bahari, Kiew Lian Wan, Mohd Noor Mat Isa, Siang Hee Tan, K Harikrishna, Hoong Yeet YeangAbstract:Hevea Brasiliensis is the most widely cultivated species for commercial production of natural rubber (cis-polyisoprene). In this study, 10 040 expressed sequence tags (ESTs) were generated from the latex of the rubber tree, which represents the cytoplasmic content of a single cell type, in order to analyse the latex transcription profile with emphasis on rubber biosynthesis-related genes. A total of 3441 unique transcripts (UTs) were obtained after quality editing and assembly of EST sequences. Functional classification of UTs according to the Gene Ontology convention showed that 73.8% were related to genes of unknown function. Among highly expressed ESTs, a significant proportion encoded proteins related to rubber biosynthesis and stress or defence responses. Sequences encoding rubber particle membrane proteins (RPMPs) belonging to three protein families accounted for 12% of the ESTs. Characterization of these ESTs revealed nine RPMP variants (7.9–27 kDa) including the 14 kDa REF (rubber elongation factor) and 22 kDa SRPP (small rubber particle protein). The expression of multiple RPMP isoforms in latex was shown using antibodies against REF and SRPP. Both EST and quantitative reverse transcription-PCR (QRTPCR) analyses demonstrated REF and SRPP to be the most abundant transcripts in latex. Besides rubber biosynthesis, comparative sequence analysis showed that the RPMPs are highly similar to sequences in the plant kingdom having stress-related functions. Implications of the RPMP function in cis-polyisoprene biosynthesis in the context of transcript abundance and differential gene expression are discussed.
-
isolation and characterization of the early nodule specific protein homologue hev b 13 an allergenic lipolytic esterase from Hevea Brasiliensis latex
The Journal of Allergy and Clinical Immunology, 2004Co-Authors: S A M Arif, Robert G Hamilton, Faridah Yusof, Nyu Ping Chew, Y H Loke, Subodh B Nimkar, Jaap J Beintema, Hoong Yeet YeangAbstract:Abstract Rationale Identification of Hev b 7, a 43 kDa latex allergen homologue of patatin, did not adequately explain the frequent observations of IgE antibody reactivity to a 42–46 kDa protein in serum from latex-allergic patients. We thus hypothesized the existence of another allergenic Hevea protein of a similar molecular weight. We report on the isolation and purification of a 42.98 kDa latex glycoprotein showing homology to Early Nodule Specific Protein in legumes. Methods Hev b 13 was isolated from Hevea Brasiliensis latex B-serum by gel filtration and ionic exchange chromatography. Binding of human IgE to Hev b 13 and inhibition of IgE binding by patatin were studied by Western immuno-blots analysis. The cDNA encoding Hev b 13 was isolated by RT-PCR and cloned. Results The protein predicted from the Hev b 13 cDNA sequence has 391 amino acids. The mature protein possessed a deduced molecular weight of 40.40 kDa and pI of 5.0. Prevalence of IgE antibody reactivity to native Hev b 13 was 78% (51/65) in latex-sensitized healthcare workers and 77% (10/13) in latex-sensitized spina bifida children. IgE-binding to Hev b 13 was inhibited by the potato tuber patatin but lost when the carbohydrate moiety of patatin was removed. This suggested that the IgE-specific epitope might reside on the glycan. Conclusions The lipolytic esterase, Hev b 13, is a major allergen of Hevea Brasiliensis latex. The principal IgE-specific epitope of the glycoprotein appears to be located on its carbohydrate moiety.
Frederic Peruch - One of the best experts on this subject based on the ideXlab platform.
-
highlights on Hevea Brasiliensis pro hevein proteins
Biochimie, 2016Co-Authors: Karine Berthelot, Frederic Peruch, Sophie LecomteAbstract:Hevein, from Hevea Brasiliensis (rubber tree), was identified in 1960. It is the most abundant soluble protein (22%) found in latex. Hevein is formed from a larger protein called prohevein. The 187 amino-acid prohevein is cleaved into two fragments: the N-terminal 43 amino-acid hevein, a lectin bearing a chitin-binding motif with antifungal properties, and a C-terminal domain (C-ter), which possesses amyloid properties. Hevein-like proteins are also widely represented in the plant kingdom and belong to a larger family related to stress and pathogenic responses. During the last 55 years, these proteins have attracted the interest of numerous specialists from the fields of plant physiology, genetics, molecular and structural biology, and physico-chemistry to allergology. This review highlights various aspects of hevein, prohevein, and C-ter from the point of view of these various fields, and examines their potential roles in latex as well as their beneficial and negative biological effects (e.g. wound sealing and resistance to pathogens which is mediated by agglutination, antimicrobial activity, and/or allergenicity). It covers results and observations from 1960 up to the most recent research.
-
Hevea Brasiliensis REF (Hev b 1) and SRPP (Hev b 3): An overview on rubber particle proteins
Biochimie, 2014Co-Authors: Karine Berthelot, Yannick Estevez, Sophie Lecomte, Frederic PeruchAbstract:This review article aims to gather all the knowledge on two important proteins associated with Hevea Brasiliensis rubber particles: namely the rubber elongation factor (REF) and the small rubber particle protein (SRPP). It covers more then three decades of research on these two proteins and their homologues in plants, and particularly emphasizes on the different possible properties or functions of these various proteins found in plants.