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Jiann-chu Chen - One of the best experts on this subject based on the ideXlab platform.

  • white shrimp litopenaeus vannamei that had received the Hot Water Extract of spirulina platensis showed earlier recovery in immunity and up regulation of gene expressions after ph stress
    Fish & Shellfish Immunology, 2010
    Co-Authors: Yongchin Lin, Chienlun Huang, Carina Miranda Tayag, Wenching Tsui, Jiann-chu Chen
    Abstract:

    White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰, pH 8.2) containing the Hot-Water Extract of Spirulina platensis at 0 (control), 200, 400, and 600 mg L(-1) for 3 h, were transferred to seaWater at pH 6.8, and the immune parameters and transcripts of the lipopolysaccharide- and β-glucan-binding protein (LGBP), peroxinectin (PX), and integrin β (IB) were examined 6-96 h post-transfer. Shrimp with no exposure to the Hot-Water Extract and no pH change served as the background control. Results indicated that the hyaline cells, granular cells (including semi-granular cells), total haemocyte count, phenoloxidase activity, respiratory burst, superoxide dismutase activity, glutathione peroxidase activity, and lysozyme activity of shrimp transferred to seaWater at pH 6.8 significantly decreased to the lowest at 6 h post-transfer. These immune parameters of shrimp immersed in 600 mg L(-1) of the Extract were significantly higher than those of control shrimp at 24-96 h post-transfer, and had returned to the background values earlier at 48-72 h post-transfer with significant transcripts of LGBP, PX, and IB at 24, 6, and 24 h, respectively, whereas these parameters of control shrimp returned to the original values at ≥96 h post-transfer.

  • the protective immunity of white shrimp litopenaeus vannamei that had been immersed in the Hot Water Extract of gracilaria tenuistipitata and subjected to combined stresses of vibrio alginolyticus injection and temperature change
    Fish & Shellfish Immunology, 2010
    Co-Authors: Su-tuen Yeh, Yongchin Lin, Wenching Tsui, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰) containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 400, and 600 mg L −1 for 3 h, were subjected to temperature transfer (28 °C), or combined stresses of Vibrio alginolyticus injection (2.4 × 10 6 colony-forming unit shrimp −1 ) and temperature transfer (28 °C) from 24 °C, and the immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, and haemolymph protein concentration were examined 6–144 h post-transfer. Shrimp with no exposure to the Extract and no temperature transfer served as the background control. Results indicated that these parameters of shrimp subjected to temperature transfer, or subjected to combined stresses significantly decreased to the lowest at 12 h post-transfer. Results indicated that these parameters of shrimp immersed in 600 mg l −1 Extract had returned to the background values at 24–144 h post-transfer, whereas these parameters of control shrimp returned to the background values at  ≥144 h post-transfer. It was therefore concluded that the immunity of L. vannamei which had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited a protective effect against temperature transfer, and combined stresses of V. alginolyticus injection and temperature transfer as evidenced by the earlier recovery of immune parameters.

  • white shrimp litopenaeus vannamei that received the Hot Water Extract of gracilaria tenuistipitata showed protective innate immunity and up regulation of gene expressions after low salinity stress
    Fish & Shellfish Immunology, 2010
    Co-Authors: Su-tuen Yeh, Yongchin Lin, Chienlun Huang, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰) containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 200, 400, and 600 mg L −1 for 3 h, were subjected to a salinity transfer to 25‰, and the immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, haemolymph protein concentration, and transcripts of the lipopolysaccharide- and β-glucan-binding protein (LGBP), peroxinectin (PX), and α2-macroglobulin (α2-M) were examined 6–96 h post-transfer. Shrimp with no exposure to the Hot-Water Extract and no salinity transfer served as the background control. Results indicated that HCs, GCs, THC, PO activity, RB, SOD activity, and haemolymph protein concentration of shrimp immersed in 600 mg L −1 Extract were significantly higher than those of control shrimp at 6–12 h post-transfer. Results also indicated that these parameters of shrimp immersed in 600 mg L −1 Extract had returned to the background values at 12, 6, 12, 6, 12, 24, and 24 h post-transfer with significant transcripts of LGBP, PX, and α2-M at 12 h, whereas these immune parameters in control shrimp had returned to the original values at 96 h post-transfer. It was therefore concluded that the innate immunity of L. vannamei which had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited a protective effect against low-salinity stress as evidenced by increases in LGBP, PX, and α2-M transcripts, and earlier recovery of immune parameters.

  • administration of the Hot Water Extract of spirulina platensis enhanced the immune response of white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus
    Fish & Shellfish Immunology, 2010
    Co-Authors: Carina Miranda Tayag, Yongchin Lin, Chynghwa Liou, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been injected with the Hot-Water Extract of Spirulina platensis at 6, 10, and 20 μg g −1 , or immersed in aerated seaWater containing Extract at 200, 400, and 600 mg L −1 were challenged with Vibrio alginolyticus at 1.5 × 10 6 or 1.4 × 10 6 colony-forming units (cfu) shrimp −1 , and then placed in seaWater. Survival rates of shrimp that received the Extract of S. platensis at 6–20 μg g −1 , and those of shrimp immersed in seaWater containing the Extract at 400 and 600 mg L −1 were significantly higher than those of control shrimp after 24–96 and 48–96 h, respectively. In a separate experiment, the hyaline cell (HC) count, granular cell (GC, including semi-granular cell) count, total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, and lysozyme activity were measured when shrimp were injected with the Extract at 6, 10, and 20 μg g −1 , and immersed in seaWater containing the Extract at 200, 400, and 600 mg L −1 . These parameters directly increased with the concentration, and significantly increased when shrimp were immersed in the seaWater containing the Extract at 0.5–4 h. L. vannamei that received all doses of the Extract via injection or via immersion all had increased phagocytic activity and clearance efficiency to V. alginolyticus at 12–72 h and 3–4 h, respectively. It was concluded that L. vannamei that received the Hot-Water Extract of S. platensis had enhanced innate immunity and increased resistance against V. alginolyticus infection.

  • white shrimp litopenaeus vannamei that received the Hot Water Extract of gracilaria tenuistipitata showed earlier recovery in immunity after a vibrio alginolyticus injection
    Fish & Shellfish Immunology, 2009
    Co-Authors: Su-tuen Yeh, Jiann-chu Chen
    Abstract:

    White shrimp Litopenaeus vannamei which had been immersed in seaWater containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 200, 400, and 600 mg L(-1) for 3 h, were challenged with Vibrio alginolyticus at 4.6 x 10(6) colony-forming units (CFU) shrimp(-1) and then placed in normal seaWater (34 per thousand). The survival rates of shrimp immersed in 200, 400, and 600 mg L(-1) of the Hot-Water Extract were significantly higher than those of control shrimp over 48-120 h. In another experiment, L. vannamei which had been immersed in the Hot-Water Extract at 0, 200, 400, and 600 mg L(-1) for 3 h, were challenged with V. alginolyticus at 4.0 x 10(6) CFU shrimp(-1), and the immune parameters examined included the haemocyte count, phenoloxidase (PO) activity, respiratory burst (RB), and superoxide dismutase (SOD) activity at 12-120 h post-challenge after shrimp had been released into normal seaWater. Shrimp not exposed to the Hot-Water Extract or V. alginolyticus served as the background control. Results indicated that the haemocyte count, PO activity, RB, and SOD activity of shrimp immersed in 600 mg L(-1) were significantly higher than those of control shrimp at 12-72 h post-challenge. Results also indicated that total haemocyte count (THC), PO activity, RB and SOD activity of shrimp immersed in 400 and 600 mg L(-1) of the Hot-Water Extract returned to the background values at 96, 48, 48, and 72 h, whereas these parameters of control shrimp returned to the original values at >120, >120, 96, and 96 h post-challenge, respectively. It was therefore concluded that L. vannamei that had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited protection against V. alginolyticus as evidenced by the earlier recovery of immune parameters.

Su-tuen Yeh - One of the best experts on this subject based on the ideXlab platform.

  • the protective immunity of white shrimp litopenaeus vannamei that had been immersed in the Hot Water Extract of gracilaria tenuistipitata and subjected to combined stresses of vibrio alginolyticus injection and temperature change
    Fish & Shellfish Immunology, 2010
    Co-Authors: Su-tuen Yeh, Yongchin Lin, Wenching Tsui, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰) containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 400, and 600 mg L −1 for 3 h, were subjected to temperature transfer (28 °C), or combined stresses of Vibrio alginolyticus injection (2.4 × 10 6 colony-forming unit shrimp −1 ) and temperature transfer (28 °C) from 24 °C, and the immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, and haemolymph protein concentration were examined 6–144 h post-transfer. Shrimp with no exposure to the Extract and no temperature transfer served as the background control. Results indicated that these parameters of shrimp subjected to temperature transfer, or subjected to combined stresses significantly decreased to the lowest at 12 h post-transfer. Results indicated that these parameters of shrimp immersed in 600 mg l −1 Extract had returned to the background values at 24–144 h post-transfer, whereas these parameters of control shrimp returned to the background values at  ≥144 h post-transfer. It was therefore concluded that the immunity of L. vannamei which had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited a protective effect against temperature transfer, and combined stresses of V. alginolyticus injection and temperature transfer as evidenced by the earlier recovery of immune parameters.

  • white shrimp litopenaeus vannamei that received the Hot Water Extract of gracilaria tenuistipitata showed protective innate immunity and up regulation of gene expressions after low salinity stress
    Fish & Shellfish Immunology, 2010
    Co-Authors: Su-tuen Yeh, Yongchin Lin, Chienlun Huang, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰) containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 200, 400, and 600 mg L −1 for 3 h, were subjected to a salinity transfer to 25‰, and the immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, haemolymph protein concentration, and transcripts of the lipopolysaccharide- and β-glucan-binding protein (LGBP), peroxinectin (PX), and α2-macroglobulin (α2-M) were examined 6–96 h post-transfer. Shrimp with no exposure to the Hot-Water Extract and no salinity transfer served as the background control. Results indicated that HCs, GCs, THC, PO activity, RB, SOD activity, and haemolymph protein concentration of shrimp immersed in 600 mg L −1 Extract were significantly higher than those of control shrimp at 6–12 h post-transfer. Results also indicated that these parameters of shrimp immersed in 600 mg L −1 Extract had returned to the background values at 12, 6, 12, 6, 12, 24, and 24 h post-transfer with significant transcripts of LGBP, PX, and α2-M at 12 h, whereas these immune parameters in control shrimp had returned to the original values at 96 h post-transfer. It was therefore concluded that the innate immunity of L. vannamei which had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited a protective effect against low-salinity stress as evidenced by increases in LGBP, PX, and α2-M transcripts, and earlier recovery of immune parameters.

  • white shrimp litopenaeus vannamei that received the Hot Water Extract of gracilaria tenuistipitata showed earlier recovery in immunity after a vibrio alginolyticus injection
    Fish & Shellfish Immunology, 2009
    Co-Authors: Su-tuen Yeh, Jiann-chu Chen
    Abstract:

    White shrimp Litopenaeus vannamei which had been immersed in seaWater containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 200, 400, and 600 mg L(-1) for 3 h, were challenged with Vibrio alginolyticus at 4.6 x 10(6) colony-forming units (CFU) shrimp(-1) and then placed in normal seaWater (34 per thousand). The survival rates of shrimp immersed in 200, 400, and 600 mg L(-1) of the Hot-Water Extract were significantly higher than those of control shrimp over 48-120 h. In another experiment, L. vannamei which had been immersed in the Hot-Water Extract at 0, 200, 400, and 600 mg L(-1) for 3 h, were challenged with V. alginolyticus at 4.0 x 10(6) CFU shrimp(-1), and the immune parameters examined included the haemocyte count, phenoloxidase (PO) activity, respiratory burst (RB), and superoxide dismutase (SOD) activity at 12-120 h post-challenge after shrimp had been released into normal seaWater. Shrimp not exposed to the Hot-Water Extract or V. alginolyticus served as the background control. Results indicated that the haemocyte count, PO activity, RB, and SOD activity of shrimp immersed in 600 mg L(-1) were significantly higher than those of control shrimp at 12-72 h post-challenge. Results also indicated that total haemocyte count (THC), PO activity, RB and SOD activity of shrimp immersed in 400 and 600 mg L(-1) of the Hot-Water Extract returned to the background values at 96, 48, 48, and 72 h, whereas these parameters of control shrimp returned to the original values at >120, >120, 96, and 96 h post-challenge, respectively. It was therefore concluded that L. vannamei that had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited protection against V. alginolyticus as evidenced by the earlier recovery of immune parameters.

  • the immunostimulatory effects of Hot Water Extract of gelidium amansii via immersion injection and dietary administrations on white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus
    Fish & Shellfish Immunology, 2007
    Co-Authors: Wenying Hou, Su-tuen Yeh, Jiann-chu Chen
    Abstract:

    Abstract The total haemocyte count (THC), phenoloxidase activity, and respiratory burst were examined when white shrimp Litopenaeus vannamei were immersed in seaWater (34‰) containing Hot-Water Extract of red alga Gelidium amansii at 200, 400 and 600 mg l −1 , injected with Hot-Water Extract at 4 and 6 μg g −1 shrimp, and fed diets containing Hot-Water Extract at 0, 0.5, 1.0 and 2.0 g kg −1 . These parameters increased significantly when shrimp were immersed in seaWater containing Hot-Water Extract at 400 and 600 mg l −1 after 1 h, when shrimp were injected with Hot-Water Extract at 6 μg g −1 shrimp after one day, and when shrimp were fed diets containing Hot-Water Extract at 1.0 and 2.0 g kg −1 after 14 days. Phagocytic activity and clearance efficiency were significantly higher for the shrimp that were fed diets containing Hot-Water Extract at 1.0 and 2.0 g kg −1 than those of shrimp that were fed diets containing Hot-Water Extract at 0 and 0.5 g kg −1 after 14 and 28 days. In a separate experiment, L. vannamei which had received Hot-Water Extract via injection, or fed diets containing Hot-Water Extract, were challenged after 3 h or 28 days with V. alginolyticus at 2 × 10 6  cfu shrimp −1 and 1 × 10 6  cfu shrimp −1 , respectively, and then placed in seaWater. The survival of shrimp that were injected with Hot-Water Extract at 6 μg g −1 was significantly higher than that of control shrimp after 1 day, and the survival of shrimp fed diets containing Hot-Water Extract at 0.5, 1.0 and 2.0 g kg −1 increased significantly after 3 days as well as at the end of the experiment (6 days after the challenge), respectively. It was concluded that L. vannamei that were immersed in Hot-Water Extract at 400 mg l −1 , injected with Hot-Water Extract at 6 μg g −1 shrimp, and fed Hot-Water Extract of G. amansii at 2.0 g kg −1 or less showed increased immune ability as well as resistance to V. alginolyticus infection.

  • Administration of Hot-Water Extract of brown seaweed Sargassum duplicatum via immersion and injection enhances the immune resistance of white shrimp Litopenaeus vannamei.
    Fish & shellfish immunology, 2006
    Co-Authors: Su-tuen Yeh, Chiu-sha Lee, Jiann-chu Chen
    Abstract:

    Abstract The total haemocyte count (THC), phenoloxidase activity, and respiratory burst were examined when the white shrimp Litopenaeus vannamei (10.42±1.39 g) were immersed in seaWater (34‰) containing Hot-Water Extract of brown alga Sargassum duplicatum at 100, 300 and 500 mg l −1 , or injected with Hot-Water Extract of S. duplicatum at 2, 6, 10 and 20 μg g −1 . These parameters increased significantly when the shrimp were immersed in seaWater containing Hot-Water Extract at 300 and 500 mg l −1 after 1 h, or when the shrimp were injected with Hot-Water Extract at 10 and 20 μg g −1 after 1 day. L. vannamei that were injected with Hot-Water Extract at 6, 10 and 20 μg g −1 had increased phagocytic activity and clearance efficiency to V. alginolyticus after 1–6 days. In another experiment, L. vannamei which had been immersed in seaWater containing Hot-Water Extract at 100, 300 and 500 mg l −1 , or injected with Hot-Water Extract at 2, 6, 10 or 20 μg g −1 were challenged with V. alginolyticus at 1×10 6 , or 1.4×10 6 colony-forming units (cfu) shrimp −1 , and then placed in seaWater. The survival of shrimp that received Hot-Water Extract at either dose was significantly higher than that of control shrimp after 2 days, as well as at the termination of the experiment (6 days after the challenge). It is therefore concluded that L. vannamei that were immersed in Hot-Water Extract of S. duplicatum at 300 mg l −1 , or the shrimp that were injected with Hot-Water Extract at 10 μg g −1 or less had increased immune ability as well as resistance to V. alginolyticus infection.

Kuninori Shiwaku - One of the best experts on this subject based on the ideXlab platform.

  • anti obesity effects of Hot Water Extract from wasabi wasabia japonica matsum leaves in mice fed high fat diets
    Nutrition Research and Practice, 2013
    Co-Authors: Masayuki Yamasaki, Tetsuro Ogawa, Takuya Katsube, Yukikazu Yamasaki, Li Wang, Kuninori Shiwaku
    Abstract:

    The anti-obesity effects of a Hot Water Extract from wasabi (Wasabia japonica Matsum.) leaves (WLE), without its specific pungent constituents, such as allyl-isothiocyanate, were investigated in high fat-diet induced mice. C57J/BL mice were fed a high-fat diet (control group) or a high-fat diet supplemented with 5% WLE (WLE group). Physical parameters and blood profiles were determined. Gene expression associated with lipid metabolism in liver and white adipose tissue were analyzed. After 120 days of feeding, significantly lower body weight gain, liver weight and epididymal white adipose tissue weight was observed in the WLE group compared to the control group. In liver gene expression within the WLE group, PPARα was significantly enhanced and SREBP-1c was significantly suppressed. Subsequent downstream genes controlled by these regulators were significantly suppressed. In epididymal white adipose tissue of the WLE group, expression of leptin, PPARγ, and C/EBPα were significantly suppressed and adiponectin was significantly enhanced. Acox, related to fatty acid oxidization in adipocytes, was also enhanced. Our results demonstrate that the WLE dietary supplement induces mild suppression of obesity in a high-fat diet induced mice, possibly due to suppression of lipid accumulation in liver and white adipose tissue.

  • suppressive effect of Hot Water Extract of wasabi wasabia japonica matsum leaves on the differentiation of 3t3 l1 preadipocytes
    Food Chemistry, 2010
    Co-Authors: Tetsuro Ogawa, Takuya Katsube, Hiromasa Tabata, Yukari Ohta, Yukikazu Yamasaki, Masayuki Yamasaki, Kuninori Shiwaku
    Abstract:

    This study investigated the effect of Hot Water Extract of wasabi (Wasabia japonica Matsum.) leaves (WLE), without its specific pungent constituents, such as allyl isothiocyanate, on the differentiation of 3T3-L1 preadipocytes. WLE suppressed the increase in glycerol-3-phosphate dehydrogenase (GPDH) activity and triglyceride (TG) accumulation, markers of adipogenesis, in a dose-dependent manner. Quantitative real time RT-PCR results showed that WLE significantly reduced the mRNA expression levels of peroxisome proliferator-activated receptor (PPAR) γ and CCAAT/enhancer-binding protein (C/EBP) α, both key adipogenic transcription factors, as subsequently were the mRNA expression levels of their target genes, such as adipocyte fatty acid binding protein 2 (aP2). Western blot analysis results showed that the protein expression levels of both PPARγ and C/EBPα were also inhibited by WLE. Thus, WLE suppressed the differentiation of 3T3-L1 preadipocytes, and the suppressive effect was mediated, in part, through the altered regulation of PPARγ, C/EBPα, and other specific genes, such as aP2. These results suggest that WLE may prevent obesity and insulin resistance by inhibiting the differentiation of preadipocytes.

Yongchin Lin - One of the best experts on this subject based on the ideXlab platform.

  • white shrimp litopenaeus vannamei that had received the Hot Water Extract of spirulina platensis showed earlier recovery in immunity and up regulation of gene expressions after ph stress
    Fish & Shellfish Immunology, 2010
    Co-Authors: Yongchin Lin, Chienlun Huang, Carina Miranda Tayag, Wenching Tsui, Jiann-chu Chen
    Abstract:

    White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰, pH 8.2) containing the Hot-Water Extract of Spirulina platensis at 0 (control), 200, 400, and 600 mg L(-1) for 3 h, were transferred to seaWater at pH 6.8, and the immune parameters and transcripts of the lipopolysaccharide- and β-glucan-binding protein (LGBP), peroxinectin (PX), and integrin β (IB) were examined 6-96 h post-transfer. Shrimp with no exposure to the Hot-Water Extract and no pH change served as the background control. Results indicated that the hyaline cells, granular cells (including semi-granular cells), total haemocyte count, phenoloxidase activity, respiratory burst, superoxide dismutase activity, glutathione peroxidase activity, and lysozyme activity of shrimp transferred to seaWater at pH 6.8 significantly decreased to the lowest at 6 h post-transfer. These immune parameters of shrimp immersed in 600 mg L(-1) of the Extract were significantly higher than those of control shrimp at 24-96 h post-transfer, and had returned to the background values earlier at 48-72 h post-transfer with significant transcripts of LGBP, PX, and IB at 24, 6, and 24 h, respectively, whereas these parameters of control shrimp returned to the original values at ≥96 h post-transfer.

  • the protective immunity of white shrimp litopenaeus vannamei that had been immersed in the Hot Water Extract of gracilaria tenuistipitata and subjected to combined stresses of vibrio alginolyticus injection and temperature change
    Fish & Shellfish Immunology, 2010
    Co-Authors: Su-tuen Yeh, Yongchin Lin, Wenching Tsui, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰) containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 400, and 600 mg L −1 for 3 h, were subjected to temperature transfer (28 °C), or combined stresses of Vibrio alginolyticus injection (2.4 × 10 6 colony-forming unit shrimp −1 ) and temperature transfer (28 °C) from 24 °C, and the immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, and haemolymph protein concentration were examined 6–144 h post-transfer. Shrimp with no exposure to the Extract and no temperature transfer served as the background control. Results indicated that these parameters of shrimp subjected to temperature transfer, or subjected to combined stresses significantly decreased to the lowest at 12 h post-transfer. Results indicated that these parameters of shrimp immersed in 600 mg l −1 Extract had returned to the background values at 24–144 h post-transfer, whereas these parameters of control shrimp returned to the background values at  ≥144 h post-transfer. It was therefore concluded that the immunity of L. vannamei which had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited a protective effect against temperature transfer, and combined stresses of V. alginolyticus injection and temperature transfer as evidenced by the earlier recovery of immune parameters.

  • white shrimp litopenaeus vannamei that received the Hot Water Extract of gracilaria tenuistipitata showed protective innate immunity and up regulation of gene expressions after low salinity stress
    Fish & Shellfish Immunology, 2010
    Co-Authors: Su-tuen Yeh, Yongchin Lin, Chienlun Huang, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been immersed in seaWater (35‰) containing the Hot-Water Extract of Gracilaria tenuistipitata at 0 (control), 200, 400, and 600 mg L −1 for 3 h, were subjected to a salinity transfer to 25‰, and the immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, haemolymph protein concentration, and transcripts of the lipopolysaccharide- and β-glucan-binding protein (LGBP), peroxinectin (PX), and α2-macroglobulin (α2-M) were examined 6–96 h post-transfer. Shrimp with no exposure to the Hot-Water Extract and no salinity transfer served as the background control. Results indicated that HCs, GCs, THC, PO activity, RB, SOD activity, and haemolymph protein concentration of shrimp immersed in 600 mg L −1 Extract were significantly higher than those of control shrimp at 6–12 h post-transfer. Results also indicated that these parameters of shrimp immersed in 600 mg L −1 Extract had returned to the background values at 12, 6, 12, 6, 12, 24, and 24 h post-transfer with significant transcripts of LGBP, PX, and α2-M at 12 h, whereas these immune parameters in control shrimp had returned to the original values at 96 h post-transfer. It was therefore concluded that the innate immunity of L. vannamei which had been immersed in seaWater containing the Hot-Water Extract of G. tenuistipitata exhibited a protective effect against low-salinity stress as evidenced by increases in LGBP, PX, and α2-M transcripts, and earlier recovery of immune parameters.

  • administration of the Hot Water Extract of spirulina platensis enhanced the immune response of white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus
    Fish & Shellfish Immunology, 2010
    Co-Authors: Carina Miranda Tayag, Yongchin Lin, Chynghwa Liou, Jiann-chu Chen
    Abstract:

    Abstract White shrimp Litopenaeus vannamei which had been injected with the Hot-Water Extract of Spirulina platensis at 6, 10, and 20 μg g −1 , or immersed in aerated seaWater containing Extract at 200, 400, and 600 mg L −1 were challenged with Vibrio alginolyticus at 1.5 × 10 6 or 1.4 × 10 6 colony-forming units (cfu) shrimp −1 , and then placed in seaWater. Survival rates of shrimp that received the Extract of S. platensis at 6–20 μg g −1 , and those of shrimp immersed in seaWater containing the Extract at 400 and 600 mg L −1 were significantly higher than those of control shrimp after 24–96 and 48–96 h, respectively. In a separate experiment, the hyaline cell (HC) count, granular cell (GC, including semi-granular cell) count, total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, and lysozyme activity were measured when shrimp were injected with the Extract at 6, 10, and 20 μg g −1 , and immersed in seaWater containing the Extract at 200, 400, and 600 mg L −1 . These parameters directly increased with the concentration, and significantly increased when shrimp were immersed in the seaWater containing the Extract at 0.5–4 h. L. vannamei that received all doses of the Extract via injection or via immersion all had increased phagocytic activity and clearance efficiency to V. alginolyticus at 12–72 h and 3–4 h, respectively. It was concluded that L. vannamei that received the Hot-Water Extract of S. platensis had enhanced innate immunity and increased resistance against V. alginolyticus infection.

Shahidul Islam - One of the best experts on this subject based on the ideXlab platform.

  • the antidiabetic properties of the Hot Water Extract of kola nut cola nitida vent scHott endl in type 2 diabetic rats
    Journal of Ethnopharmacology, 2019
    Co-Authors: Ochuko L. Erukainure, Omamuyovwi M Ijomone, Collins U Ibeji, Chika I Chukwuma, Olakunle Sanni, Shahidul Islam
    Abstract:

    Abstract Ethnopharmacological relevance Cola nitida is amongst the evergreen plants native to West Africa used in the treatment of various ailments including diabetes. Aim of the study: This study aims to investigate the antidiabetic effects of the Hot Water Extract of C. nitida seeds in type 2 diabetic rats. Methods Type 2 diabetic rats were orally administered with low (150 mg/kg bw) and high (300 mg/kg bw) doses of the Hot Water Extract for 6 wk and thereafter, blood glucose, insulin level, lipid profile, pancreatic β-cell function, perfusion and morphology, redox imbalance, glycolytic and cholinergic enzymes, as well as of caspase-3 and Nrf2 expressions were measured. Results Treatment with the Extract led to significant depletion of blood glucose, serum triglycerides, LDL-cholesterol, fructosamine, ALT, and uric acids, while elevating serum insulin and HDL-cholesterol levels. The infusion also significantly (p  Conclusion These results insinuate the antidiabetic activities of C. nitida Hot Water Extract and may be attributed to the NMR-identified compounds.

  • The antidiabetic properties of the Hot Water Extract of kola nut (Cola nitida (Vent.) ScHott & Endl.) in type 2 diabetic rats.
    Journal of Ethnopharmacology, 2019
    Co-Authors: Ochuko L. Erukainure, Omamuyovwi M Ijomone, Collins U Ibeji, Chika I Chukwuma, Olakunle Sanni, Shahidul Islam
    Abstract:

    Abstract Ethnopharmacological relevance Cola nitida is amongst the evergreen plants native to West Africa used in the treatment of various ailments including diabetes. Aim of the study: This study aims to investigate the antidiabetic effects of the Hot Water Extract of C. nitida seeds in type 2 diabetic rats. Methods Type 2 diabetic rats were orally administered with low (150 mg/kg bw) and high (300 mg/kg bw) doses of the Hot Water Extract for 6 wk and thereafter, blood glucose, insulin level, lipid profile, pancreatic β-cell function, perfusion and morphology, redox imbalance, glycolytic and cholinergic enzymes, as well as of caspase-3 and Nrf2 expressions were measured. Results Treatment with the Extract led to significant depletion of blood glucose, serum triglycerides, LDL-cholesterol, fructosamine, ALT, and uric acids, while elevating serum insulin and HDL-cholesterol levels. The infusion also significantly (p  Conclusion These results insinuate the antidiabetic activities of C. nitida Hot Water Extract and may be attributed to the NMR-identified compounds.