The Experts below are selected from a list of 3387 Experts worldwide ranked by ideXlab platform
Guangming Zhong - One of the best experts on this subject based on the ideXlab platform.
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complement factor c5 but not c3 contributes significantly to Hydrosalpinx development in mice infected with chlamydia muridarum
Infection and Immunity, 2014Co-Authors: Zhangsheng Yang, Turner Conrad, Zhou Zhou, Jianlin Chen, Pavel Dutow, Andreas Klos, Guangming ZhongAbstract:ABSTRACT Hydrosalpinx is a pathological hallmark of tubal infertility associated with chlamydial infection. However, the mechanisms of Hydrosalpinx remain unknown. Here, we report that complement factor 5 (C5) contributes significantly to chlamydial induction of Hydrosalpinx. Mice lacking C5 (C5 −/− ) failed to develop any Hydrosalpinx, while ∼42% of the corresponding wild-type mice (C5 +/+ ) did so following intravaginal infection with Chlamydia muridarum. Surprisingly, deficiency in C3 (C3 −/− ), an upstream component of the complement system, did not affect mouse susceptibility to chlamydial induction of Hydrosalpinx. Interestingly, C5 activation was induced by chlamydial infection in oviducts of C3 −/− mice, explaining why the C3 −/− mice remained susceptible to chlamydial induction of Hydrosalpinx. Similar levels of live chlamydial organisms were recovered from oviduct tissues of both C5 −/− and C5 +/+ mice, suggesting that C5 deficiency did not affect C. muridarum ascending infection. Furthermore, C5 −/− mice were still more resistant to Hydrosalpinx induction than C5 +/+ mice, even when live C. muridarum organisms were directly delivered into the upper genital tract, both confirming the role of C5 in promoting Hydrosalpinx and indicating that the C5-facilitated Hydrosalpinx was not due to enhancement of ascending infection. The C5 −/− mice displayed significantly reduced lumenal inflammatory infiltration and cytokine production in oviduct tissue, suggesting that C5 may contribute to chlamydial induction of Hydrosalpinx by enhancing inflammatory responses.
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signaling via tumor necrosis factor receptor 1 but not toll like receptor 2 contributes significantly to Hydrosalpinx development following chlamydia muridarum infection
Infection and Immunity, 2014Co-Authors: Xiaotong Chang, Xiaohua Dong, Guangming ZhongAbstract:ABSTRACT Chlamydial infection in the lower genital tract can lead to Hydrosalpinx, which is accompanied by activation of both pattern recognition receptor TLR2- and inflammatory cytokine receptor TNFR1-mediated signaling pathways. In the current study, we compared the relative contributions of these two receptors to chlamydial induction of Hydrosalpinx in mice. We found that mice with or without deficiencies in TLR2 or TNFR1 displayed similar time courses of live organism shedding from vaginal swabs, suggesting that these receptor-mediated signaling pathways are not required for controlling chlamydial lower genital infection. However, mice deficient in TNFR1 but not TLR2 developed significantly reduced Hydrosalpinx. The decreased pathogenicity correlated with a significant reduction in interleukin-17 by in vitro -restimulated splenocytes of TNFR1-deficient mice. Although TLR2-deficient mice developed Hydrosalpinx as severe as that of wild-type mice, peritoneal macrophages from mice deficient in TLR2 but not TNFR1 produced significantly reduced cytokines upon chlamydial stimulation, suggesting that reduced macrophage responses to chlamydial infection do not always lead to a reduction in Hydrosalpinx. Thus, we have demonstrated that the signaling pathways triggered by the cytokine receptor TNFR1 play a more significant role in chlamydial induction of Hydrosalpinx than those mediated by the pattern recognition receptor TLR2, which has laid a foundation for further revealing the chlamydial pathogenic mechanisms.
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reduced live organism recovery and lack of Hydrosalpinx in mice infected with plasmid free chlamydia muridarum
Infection and Immunity, 2014Co-Authors: Zhangsheng Yang, Jianlin Chen, Joel B Baseman, Yiling Ding, Hao Zeng, Guangming ZhongAbstract:ABSTRACT Plasmid-free Chlamydia trachomatis and Chlamydia muridarum fail to induce severe pathology. To evaluate whether the attenuated pathogenicity is due to insufficient infection or inability of the plasmidless chlamydial organisms to trigger pathological responses, we compared plasmid-competent and plasmid-free C. muridarum infections in 5 different strains of mice. All 5 strains developed Hydrosalpinx following intravaginal inoculation with plasmid-competent, but not inoculation with plasmid-free, C. muridarum. The lack of Hydrosalpinx induction by plasmid-free C. muridarum correlated with significantly reduced live organism recovery from the lower genital tract and shortened infection in the upper genital tract. The plasmid-free C. muridarum organisms failed to induce Hydrosalpinx even when the organisms were directly inoculated into the oviduct via an intrabursal injection, which was accompanied by significantly reduced survival of the plasmidless organisms in the genital tracts. Furthermore, plasmid-competent C. muridarum organisms after UV inactivation were no longer able to induce Hydrosalpinx even when directly delivered into the oviduct at a high dose. Together, these observations suggest that decreased survival of and shortened infection with plasmid-free C. muridarum may contribute significantly to its attenuated pathogenicity. We conclude that adequate live chlamydial infection in the oviduct may be necessary to induce Hydrosalpinx.
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oviduct infection and Hydrosalpinx in dba1 j mice is induced by intracervical but not intravaginal inoculation with chlamydia muridarum
PLOS ONE, 2013Co-Authors: Hongbo Zhang, Zhiguang Zhou, Joel B Baseman, Lingli Tang, Siqi Gong, Guangming ZhongAbstract:Intravaginal infection with C. muridarum in mice often results in Hydrosalpinx similar to that found in women urogenitally infected with C. trachomatis, making the C. muridarum lower genital tract infection murine model suitable for studying C. trachomatis pathogenesis. To our surprise, DBA1/j mice were highly resistant to Hydrosalpinx following an intravaginal infection with C. muridarum although these mice were as susceptible to lower genital tract infection as other mouse strains. A significantly lower level of C. muridarum organisms was recovered from the oviduct of DBA1/j mice, correlating the resistance to Hydrosalpinx with reduced ascension of C. muridarum to the oviduct. The DBA1/j resistance to Hydrosalpinx was effectively overcome by intracervical inoculation with C. muridarum. The intracervically inoculated DBA1/j mice developed severe Hydrosalpinx with the highest levels of live C. muridarum organisms recovered from uterine tissue on day 3 and oviduct tissue on day 7 post inoculation while in intravaginally inoculated DBA1/j mice, the peak of live organism recovery from uterine tissue was delayed to day 7 with no rise in the amount of live organisms recovered from the oviduct. These observations have not only validated the correlation between Hydrosalpinx and live organism invasion in the oviduct but also demonstrated that the intracervical inoculation, by promoting rapid chlamydial replication in the uterine epithelial cells and ascension to the oviduct of DBA1/j mice, may be used for further understanding chlamydial pathogenic mechanisms. The above findings also suggest that strategies aimed at reducing tubal infection may be most effective in blocking tubal pathology.
Eugene Katz - One of the best experts on this subject based on the ideXlab platform.
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effect of human Hydrosalpinx fluid on murine embryo development and implantation
Fertility and Sterility, 1997Co-Authors: Vanessa J Rawe, Stefanie Shaffer, Mary G Compton, Jairo E Garcia, Eugene KatzAbstract:Abstract Objective : To determine the effect of Hydrosalpinx fluid-containing medium on murine embryo development and implantation. Design : The development of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human Hydrosalpinx fluid was observed. Implantation rates of mouse embryos transferred into the uterine horn with Hydrosalpinx fluid-containing media were determined. Setting : Private hospital-based fertility center and IVF program. Main Outcome Measure(s) : Percentage of embryos continuing cell division and implantation rates after ET. Result(s) : Hydrosalpinx fluid in culture medium affected embryo development in a dosedependent fashion. The injection of Hydrosalpinx fluid-containing medium into the uterine horn did not affect embryo implantation. Conclusion(s) : Hydrosalpinx fluid negatively affects murine embryo development, but its presence in the uterine horn at ET did not affect implantation.
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deleterious effect of the presence of Hydrosalpinx on implantation and pregnancy rates with in vitro fertilization
Fertility and Sterility, 1996Co-Authors: Eugene Katz, Mehmet Ali Akman, Marian D Damewood, Jairo E GarciaAbstract:Objective To determine the effect of the presence of a unilateral or bilateral Hydrosalpinx on the outcome with IVF-ET. Design Retrospective analysis of clinical and laboratory data. Setting Hospital-based private IVF center. Patients Eight hundred forty-six patients with tubal disease younger than age 40 years undergoing 1,766 stimulation cycles. In 118 cycles, a Hydrosalpinx was noted sonographically (group I) whereas, in 1,648 cycles, no such image was documented. Main Outcome Measures Pregnancy and implantation rates. Results Group I displayed a significantly lower pregnancy rate per transfer than group II (16.84% versus 36.83%) and a lower implantation rate (3.92% versus 11.53%). Conclusion The presence of Hydrosalpinx adversely affects the outcome of IVF.
Jianlin Chen - One of the best experts on this subject based on the ideXlab platform.
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complement factor c5 but not c3 contributes significantly to Hydrosalpinx development in mice infected with chlamydia muridarum
Infection and Immunity, 2014Co-Authors: Zhangsheng Yang, Turner Conrad, Zhou Zhou, Jianlin Chen, Pavel Dutow, Andreas Klos, Guangming ZhongAbstract:ABSTRACT Hydrosalpinx is a pathological hallmark of tubal infertility associated with chlamydial infection. However, the mechanisms of Hydrosalpinx remain unknown. Here, we report that complement factor 5 (C5) contributes significantly to chlamydial induction of Hydrosalpinx. Mice lacking C5 (C5 −/− ) failed to develop any Hydrosalpinx, while ∼42% of the corresponding wild-type mice (C5 +/+ ) did so following intravaginal infection with Chlamydia muridarum. Surprisingly, deficiency in C3 (C3 −/− ), an upstream component of the complement system, did not affect mouse susceptibility to chlamydial induction of Hydrosalpinx. Interestingly, C5 activation was induced by chlamydial infection in oviducts of C3 −/− mice, explaining why the C3 −/− mice remained susceptible to chlamydial induction of Hydrosalpinx. Similar levels of live chlamydial organisms were recovered from oviduct tissues of both C5 −/− and C5 +/+ mice, suggesting that C5 deficiency did not affect C. muridarum ascending infection. Furthermore, C5 −/− mice were still more resistant to Hydrosalpinx induction than C5 +/+ mice, even when live C. muridarum organisms were directly delivered into the upper genital tract, both confirming the role of C5 in promoting Hydrosalpinx and indicating that the C5-facilitated Hydrosalpinx was not due to enhancement of ascending infection. The C5 −/− mice displayed significantly reduced lumenal inflammatory infiltration and cytokine production in oviduct tissue, suggesting that C5 may contribute to chlamydial induction of Hydrosalpinx by enhancing inflammatory responses.
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lack of long lasting Hydrosalpinx in a j mice correlates with rapid but transient chlamydial ascension and neutrophil recruitment in the oviduct following intravaginal inoculation with chlamydia muridarum
Infection and Immunity, 2014Co-Authors: Zhangsheng Yang, Zhou Zhou, Jianlin Chen, Hongbo Zhang, Ganqiu Wu, Zhiguang Zhou, Joel B BasemanAbstract:Lower genital tract infection with Chlamydia trachomatis and C. muridarum can induce long-lasting Hydrosalpinx in the upper genital tract of women and female mice, respectively. However, A/J mice were highly resistant to induction of long-lasting Hydrosalpinx by C. muridarum. We further compared host inflammatory responses and chlamydial infection courses between the Hydrosalpinx-resistant A/J mice and CBA/J mice known to be susceptible to Hydrosalpinx induction. Both mouse strains developed robust pyosalpinx during the acute phase followed by Hydrosalpinx during the chronic phase. However, the hydrosalpinges disappeared in A/J mice by day 60 after infection, suggesting that some early hydrosalpinges are reversible. Although the overall inflammatory responses were indistinguishable between CBA/J and A/J mice, we found significantly more neutrophils in oviduct lumen of A/J mice on days 7 and 10, which correlated with a rapid but transient oviduct invasion by C. muridarum with a peak infection on day 7. In contrast, CBA/J mice developed a delayed and extensive oviduct infection. These comparisons have revealed an important role of the interactions of oviduct infection with inflammatory responses in chlamydial induction of long-lasting Hydrosalpinx, suggesting that a rapid but transient invasion of oviduct by chlamydial organisms can prevent the development of the long-lasting hydrosalpinges.
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chlamydial induction of Hydrosalpinx in 11 strains of mice reveals multiple host mechanisms for preventing upper genital tract pathology
PLOS ONE, 2014Co-Authors: Zhangsheng Yang, Zhou Zhou, Jianlin Chen, Hongbo Zhang, Zhiguang Zhou, Yiling Ding, Edward Zhong, Bernard P ArulanandamAbstract:The female lower genital tract is constantly exposed to microbial infection, some of which can ascend to and cause pathology such as Hydrosalpinx in the upper genital tract, which can affect fertility. To understand host mechanisms for preventing upper genital tract pathology, we screened 11 inbred strains of mice for Hydrosalpinx induction by C. muridarum. When examined on days 60 to 80 after intravaginal infection, the 11 strains fell into 3 groups based on their Hydrosalpinx severity: CBA/J and SJL/J mice were highly susceptible with a Hydrosalpinx score of 5 or greater; Balb/c, C57BL/6J, C57BL/10J, C3H/HeJ and C3H/HeN were susceptible with a score between 1 and <5; NOD/ShiLtJ, DBA/1J, DBA/2J and A/J were resistant with a score of <1. The diverse range of mouse susceptibility to Hydrosalpinx induction may reflect the varied clinical outcomes of C. trachomatis-infected women. When the 11 strains were infected via an intrauterine inoculation to bypass the requirement for ascension, higher incidence and more severe hydrosalpinges were induced in most mice, indicating that the interaction between chlamydial ascension and host control of ascension is critical for determining susceptibility to Hydrosalpinx development in many mice. However, a few mouse strains resisted significant exacerbation of Hydrosalpinx by intrauterine infection, indicating that these mice have evolved ascension-independent mechanisms for preventing upper genital tract pathology. Together, the above observations have demonstrated that different strains of mice can prevent upper genital tract pathology by using different mechanisms.
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reduced live organism recovery and lack of Hydrosalpinx in mice infected with plasmid free chlamydia muridarum
Infection and Immunity, 2014Co-Authors: Zhangsheng Yang, Jianlin Chen, Joel B Baseman, Yiling Ding, Hao Zeng, Guangming ZhongAbstract:ABSTRACT Plasmid-free Chlamydia trachomatis and Chlamydia muridarum fail to induce severe pathology. To evaluate whether the attenuated pathogenicity is due to insufficient infection or inability of the plasmidless chlamydial organisms to trigger pathological responses, we compared plasmid-competent and plasmid-free C. muridarum infections in 5 different strains of mice. All 5 strains developed Hydrosalpinx following intravaginal inoculation with plasmid-competent, but not inoculation with plasmid-free, C. muridarum. The lack of Hydrosalpinx induction by plasmid-free C. muridarum correlated with significantly reduced live organism recovery from the lower genital tract and shortened infection in the upper genital tract. The plasmid-free C. muridarum organisms failed to induce Hydrosalpinx even when the organisms were directly inoculated into the oviduct via an intrabursal injection, which was accompanied by significantly reduced survival of the plasmidless organisms in the genital tracts. Furthermore, plasmid-competent C. muridarum organisms after UV inactivation were no longer able to induce Hydrosalpinx even when directly delivered into the oviduct at a high dose. Together, these observations suggest that decreased survival of and shortened infection with plasmid-free C. muridarum may contribute significantly to its attenuated pathogenicity. We conclude that adequate live chlamydial infection in the oviduct may be necessary to induce Hydrosalpinx.
Hongbo Zhang - One of the best experts on this subject based on the ideXlab platform.
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lack of long lasting Hydrosalpinx in a j mice correlates with rapid but transient chlamydial ascension and neutrophil recruitment in the oviduct following intravaginal inoculation with chlamydia muridarum
Infection and Immunity, 2014Co-Authors: Zhangsheng Yang, Zhou Zhou, Jianlin Chen, Hongbo Zhang, Ganqiu Wu, Zhiguang Zhou, Joel B BasemanAbstract:Lower genital tract infection with Chlamydia trachomatis and C. muridarum can induce long-lasting Hydrosalpinx in the upper genital tract of women and female mice, respectively. However, A/J mice were highly resistant to induction of long-lasting Hydrosalpinx by C. muridarum. We further compared host inflammatory responses and chlamydial infection courses between the Hydrosalpinx-resistant A/J mice and CBA/J mice known to be susceptible to Hydrosalpinx induction. Both mouse strains developed robust pyosalpinx during the acute phase followed by Hydrosalpinx during the chronic phase. However, the hydrosalpinges disappeared in A/J mice by day 60 after infection, suggesting that some early hydrosalpinges are reversible. Although the overall inflammatory responses were indistinguishable between CBA/J and A/J mice, we found significantly more neutrophils in oviduct lumen of A/J mice on days 7 and 10, which correlated with a rapid but transient oviduct invasion by C. muridarum with a peak infection on day 7. In contrast, CBA/J mice developed a delayed and extensive oviduct infection. These comparisons have revealed an important role of the interactions of oviduct infection with inflammatory responses in chlamydial induction of long-lasting Hydrosalpinx, suggesting that a rapid but transient invasion of oviduct by chlamydial organisms can prevent the development of the long-lasting hydrosalpinges.
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chlamydial induction of Hydrosalpinx in 11 strains of mice reveals multiple host mechanisms for preventing upper genital tract pathology
PLOS ONE, 2014Co-Authors: Zhangsheng Yang, Zhou Zhou, Jianlin Chen, Hongbo Zhang, Zhiguang Zhou, Yiling Ding, Edward Zhong, Bernard P ArulanandamAbstract:The female lower genital tract is constantly exposed to microbial infection, some of which can ascend to and cause pathology such as Hydrosalpinx in the upper genital tract, which can affect fertility. To understand host mechanisms for preventing upper genital tract pathology, we screened 11 inbred strains of mice for Hydrosalpinx induction by C. muridarum. When examined on days 60 to 80 after intravaginal infection, the 11 strains fell into 3 groups based on their Hydrosalpinx severity: CBA/J and SJL/J mice were highly susceptible with a Hydrosalpinx score of 5 or greater; Balb/c, C57BL/6J, C57BL/10J, C3H/HeJ and C3H/HeN were susceptible with a score between 1 and <5; NOD/ShiLtJ, DBA/1J, DBA/2J and A/J were resistant with a score of <1. The diverse range of mouse susceptibility to Hydrosalpinx induction may reflect the varied clinical outcomes of C. trachomatis-infected women. When the 11 strains were infected via an intrauterine inoculation to bypass the requirement for ascension, higher incidence and more severe hydrosalpinges were induced in most mice, indicating that the interaction between chlamydial ascension and host control of ascension is critical for determining susceptibility to Hydrosalpinx development in many mice. However, a few mouse strains resisted significant exacerbation of Hydrosalpinx by intrauterine infection, indicating that these mice have evolved ascension-independent mechanisms for preventing upper genital tract pathology. Together, the above observations have demonstrated that different strains of mice can prevent upper genital tract pathology by using different mechanisms.
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oviduct infection and Hydrosalpinx in dba1 j mice is induced by intracervical but not intravaginal inoculation with chlamydia muridarum
PLOS ONE, 2013Co-Authors: Hongbo Zhang, Zhiguang Zhou, Joel B Baseman, Lingli Tang, Siqi Gong, Guangming ZhongAbstract:Intravaginal infection with C. muridarum in mice often results in Hydrosalpinx similar to that found in women urogenitally infected with C. trachomatis, making the C. muridarum lower genital tract infection murine model suitable for studying C. trachomatis pathogenesis. To our surprise, DBA1/j mice were highly resistant to Hydrosalpinx following an intravaginal infection with C. muridarum although these mice were as susceptible to lower genital tract infection as other mouse strains. A significantly lower level of C. muridarum organisms was recovered from the oviduct of DBA1/j mice, correlating the resistance to Hydrosalpinx with reduced ascension of C. muridarum to the oviduct. The DBA1/j resistance to Hydrosalpinx was effectively overcome by intracervical inoculation with C. muridarum. The intracervically inoculated DBA1/j mice developed severe Hydrosalpinx with the highest levels of live C. muridarum organisms recovered from uterine tissue on day 3 and oviduct tissue on day 7 post inoculation while in intravaginally inoculated DBA1/j mice, the peak of live organism recovery from uterine tissue was delayed to day 7 with no rise in the amount of live organisms recovered from the oviduct. These observations have not only validated the correlation between Hydrosalpinx and live organism invasion in the oviduct but also demonstrated that the intracervical inoculation, by promoting rapid chlamydial replication in the uterine epithelial cells and ascension to the oviduct of DBA1/j mice, may be used for further understanding chlamydial pathogenic mechanisms. The above findings also suggest that strategies aimed at reducing tubal infection may be most effective in blocking tubal pathology.
Ernest Hung Yu Ng - One of the best experts on this subject based on the ideXlab platform.
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involvement of cystic fibrosis transmembrane conductance regulator cftr in the pathogenesis of Hydrosalpinx induced by chlamydia trachomatis infection
Journal of Obstetrics and Gynaecology Research, 2008Co-Authors: Louis Chukwuemeka Ajonuma, Ernest Hung Yu Ng, Paul K S Chan, Connie Hau Yan Wong, Lai Ling Tsang, Xiao Xiao Tang, Lok Sze Ho, Chin Man Chung, Qiong He, Hong Yi HuangAbstract:Background: Genital Chlamydia (C) trachomatis infection has been recognized as the single most common cause of pelvic inflammatory disease leading to severe tubal damage, ectopic pregnancy, infertility and Hydrosalpinx. However, the mechanism underlying the formation of Hydrosalpinx induced by C. trachomatis infection remains largely unknown. We performed this study to determine the involvement of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated chloride channel that regulates epithelial electrolyte and fluid secretion, in Hydrosalpinx fluid formation. Methods: Western blot analysis was used to determine CFTR expression in the hydrosalpinges that were seen on the ultrasound scans of infertile assisted reproduction treatment patients. Correlation with C. trachomatis infection was done by testing patients' sera for C. trachomatis immunoglobulin G antibody titer using a Capita enzyme-linked immunosorbent assay based kit. CFTR involvement was further verified in a rat C. trachomatis infection model and confirmed using CFTR mutant (CFTRtm1Unc) mice. Results: Here we report on the up-regulated expression of CFTR in the Hydrosalpinx tissues of infertile patients with detectable serum levels of C. trachomatis antibody (immunoglobulin G). In a rat model, increased CFTR expression and fluid accumulation could be observed in the uterine horns infected with C. trachomatis elementary bodies, which was reversed by antibiotics treatment. In C. trachomatis–infected CFTRtm1Unc mice, however, no detectable fluid accumulation was observed. Conclusion: These findings suggest the involvement of CFTR in the pathogenesis of Hydrosalpinx fluid formation and may provide grounds for a better treatment strategy to improve assisted reproduction treatment outcome in infertile patients with Hydrosalpinx.
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new insights into the mechanisms underlying Hydrosalpinx fluid formation and its adverse effect on ivf outcome
Human Reproduction Update, 2002Co-Authors: Louis Chukwuemeka Ajonuma, Ernest Hung Yu Ng, Hsiao Chang ChanAbstract:The adverse effects of Hydrosalpinx on the outcome of IVF have been well documented; however, the causes for impaired implantation in patients with Hydrosalpinx are poorly understood. Hydrosalpinx fluid has been shown to be toxic to mouse embryos but not human embryos, and this has become a topic of intense debate. An understanding of the mechanisms underlying Hydrosalpinx formation following pelvic inflammatory disease appears to be essential in elucidating the causes for reduced implantation in Hydrosalpinx patients and providing more rational treatments. This review discusses the mechanisms underlying Hydrosalpinx formation and its adverse effect on IVF outcome, with new insights into possible involvement of Fallopian tube epithelial transporters and ion channels, particularly the cystic fibrosis transmembrane conductance regulator (CFTR). Possible links between Chlamydia trachomatis in pelvic inflammatory disease and the subsequent CFTR-mediated events in Hydrosalpinx formation leading to infertility in Hydrosalpinx are proposed. The causes of reduced implantation, particularly in patients with visible hydrosalpinges shown on ultrasound scanning, are re-examined in light of these possible mechanisms.
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adverse effects of Hydrosalpinx fluid on sperm motility and survival
Human Reproduction, 2000Co-Authors: Ernest Hung Yu Ng, Louis Chukwuemeka Ajonuma, William S B Yeung, Pak Chung HoAbstract:The negative impact of Hydrosalpinx on IVF outcome is well recognized but some reports indicate that tubal infertility with Hydrosalpinx is a heterogeneous entity and may have different effects on the outcome. The embryotoxic effects of Hydrosalpinx fluid (HF) have been documented in mouse but not human embryos. This study examined the effects of HF on sperm motility and survival after various periods of incubation. Fifteen infertile patients with Hydrosalpinx shown on ultrasound monitoring during ovarian stimulation underwent aspiration of HF after egg collection. Electrolytes, glucose and pyruvate concentrations were within the physiological ranges found in normal human tubal fluid. Sperm motility and velocities remained unchanged after 5 h of incubation with various concentrations of HF but the percentage of motile spermatozoa was significantly reduced after 24 h of incubation. Both 50 and 100% HF were potentially cytotoxic (survival indices <85%). The detrimental effect seemed to be dependent on the concentrations of HF. Low osmolarity, low lactate concentrations or the protein content may be responsible for the loss of sperm motility. A human sperm survival test using HF may be useful in selecting appropriate treatment options for patients with Hydrosalpinx undergoing IVF treatment or tubal surgery.
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Adverse effects of Hydrosalpinx fluid on sperm motility and survival
Human Reproduction, 2000Co-Authors: Ernest Hung Yu Ng, Louis Chukwuemeka Ajonuma, William S B Yeung, Pak Chung HoAbstract:The negative impact of Hydrosalpinx on IVF outcome is well recognized but some reports indicate that tubal infertility with Hydrosalpinx is a heterogeneous entity and may have different effects on the outcome. The embryotoxic effects of Hydrosalpinx fluid (HF) have been documented in mouse but not human embryos. This study examined the effects of HF on sperm motility and survival after various periods of incubation. Fifteen infertile patients with Hydrosalpinx shown on ultrasound monitoring during ovarian stimulation underwent aspiration of HF after egg collection. Electrolytes, glucose and pyruvate concentrations were within the physiological ranges found in normal human tubal fluid. Sperm motility and velocities remained unchanged after 5 h of incubation with various concentrations of HF but the percentage of motile spermatozoa was significantly reduced after 24 h of incubation. Both 50 and 100% HF were potentially cytotoxic (survival indices
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the presence of Hydrosalpinx may not adversely affect the implantation and pregnancy rates in in vitro fertilization treatment
Journal of Assisted Reproduction and Genetics, 1997Co-Authors: Ernest Hung Yu Ng, William S B Yeung, Pak Chung HoAbstract:Purpose: To evaluate the effects of Hydrosalpinx on the outcome of in vitro fertilization (IVF) treatment, a retrospective study was undertaken at a tertiary referral center for infertility. Methods: Results of the first IVF treatment cycles in 144 patients from 1 January 1993 to 31 December 1995, who had tubal infertility only and were less than 38 years old, were reviewed. The duration/dosage of hMG used, serum estradiol level on the day of hCG, number of oocytes aspirated and fertilized, number of embryos replaced, implantation rate, clinical pregnancy rate, and pregnancy outcome were compared in patients with and without Hydrosalpinx. Results: The mean implantation rate and clinical pregnancy rate were similar in patients with or without Hydrosalpinx. Both groups had similar ovarian responses and fertilization rates. There was no increase in clinical abortion in the Hydrosalpinx group but ectopic pregnancies were more common in patients with Hydrosalpinx. Conclusions: The presence of Hydrosalpinx did not adversely affect the implantation and pregnancy rates in in vitro fertilization treatment when the results of the first cycle were compared. However, it can lead to a higher incidence of ectopic pregnancies.
