The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform

Christine Coustau - One of the best experts on this subject based on the ideXlab platform.

Philippe Borsa - One of the best experts on this subject based on the ideXlab platform.

  • Single-stranded DNA conformation polymorphism at the Rdl locus in Hypothenemus Hampei (Coleoptera: Scolytidae).
    Heredity, 1996
    Co-Authors: Philippe Borsa, Christine Coustau
    Abstract:

    Single-stranded DNA conformation polymorphism at the Rdl locus in Hypothenemus Hampei (Coleoptera: Scolytidae)

  • Experimental evidence for pseudo-arrhenotoky in Hypothenemus Hampei (Coleoptera: Scolytidae)
    Heredity, 1996
    Co-Authors: Philippe Borsa, Finn Kjellberg
    Abstract:

    Species breeding under local mate competition conditions have sex ratios biased towards females, a trait that is often associated with male haploidy (arrhenotoky). We used insecticide resistance as a genetic marker in Hypothenemus Hampei , a local mate competing scolytid beetle that is cytologically diplo-diploid, for investigating the inheritance of nuclear genes. We confirmed that males express the resistance phenotype that they inherit from their mother, whatever their father. The segregation of resistance phenotypes in F_2 females significantly departed from the Mendelian model, and were in accordance with the arrhenotokous model, indicating that the parental genes were eliminated between F_1 and F_2. This finding (pseudo-arrhenotoky) constitutes a step towards an understanding of the mechanism by which diplo-diploidy evolves to arrhenotoky in local mate competing insects.

  • Single-stranded DNA conformation polymorphism at the Rdl locus in Hypothenemus Hampei (Coleoptera: Scolytidae)
    Heredity, 1996
    Co-Authors: Philippe Borsa, Christine Coustau
    Abstract:

    The homologue of the resistance to dieldrin gene (Rdl) in Drosophila melanogaster was cloned and sequenced in the scolytid beetle Hypothenemus Hampei , a coffee pest resistant to cyclo-diene insecticides in New Caledonia. The amino acid sequence of the Rdl exon no. 7 protein product in H. Hampei was identical to that in D. melanogaster and showed the same amino acid change as that characterizing susceptible vs. resistant D. melanogaster. Samples from natural H. Hampei populations (from Asia, the Pacific Islands, Africa and Central America), from reference susceptible (S) and resistant (R) laboratory strains, and from their hybrid progenies, were analysed at the Rdl locus using single-stranded DNA conformation polymorphism on polymerase chain reaction products. The susceptible allele was the only allele present in all samples from natural populations except in the only resistant population known to date (Ponerihouen, New Caledonia). Females and some males obtained as F_1 from R × S crosses were heterozygous at the Rdl locus, confirming that this local mate competing species is diplo-diploid.

Fernando E. Vega - One of the best experts on this subject based on the ideXlab platform.

  • differential metabolic responses caused by the most important insect pest of coffee worldwide the coffee berry borer Hypothenemus Hampei
    Journal of Agricultural and Food Chemistry, 2020
    Co-Authors: Fernanda R Castromoretti, Fernando E. Vega, Jeanchristophe Cocuron, Ana Paula Alonso
    Abstract:

    The world’s coffee supply is threatened by the coffee berry borer, Hypothenemus Hampei, the most destructive pest affecting coffee production and quality. This study hypothesized that coffee berry borer infestation induces distinct metabolic responses in the green coffee seeds of Coffea arabica and Coffea canephora (robusta). A targeted metabolomics approach was conducted using liquid chromatography tandem mass spectrometry to quantify intracellular metabolites in infested and uninfested arabica and robusta green seeds. In parallel, the seed biomass content and composition were assessed for the same conditions. Coffee berry borer attack induced increases in the levels of chlorogenic acids in arabica seeds, whereas organic acids and sugar alcohols were more abundant in infested robusta seeds. Most importantly, a set of compounds was identified as biomarkers differentiating the metabolic response of these taxa to the coffee berry borer.

  • Revealing the respiratory system of the coffee berry borer (Hypothenemus Hampei; Coleoptera: Curculionidae: Scolytinae) using micro-computed tomography.
    Scientific reports, 2019
    Co-Authors: Javier Alba-tercedor, Ignacio Alba-alejandre, Fernando E. Vega
    Abstract:

    The coffee berry borer (Hypothenemus Hampei) is the most economically important insect pest of coffee globally. Micro-computed tomography (micro-CT) was used to reconstruct the respiratory system of this species for the first time; this is the smallest insect (ca. 2 mm long) for which this has been done to date. Anatomical details of the spiracles and tracheal tubes are described, images presented, and new terms introduced. The total volume and the relationship between tracheal lumen diameter, length and volume are also presented. The total length of the tracheal tubes are seventy times the length of the entire animal. Videos and a 3D model for use with mobile devices are included as supplementary information; these could be useful for future research and for teaching insect anatomy to students and the public in general.

  • Anatomical study of the coffee berry borer (Hypothenemus Hampei) using micro-computed tomography
    Scientific Reports, 2019
    Co-Authors: Ignacio Alba-alejandre, Javier Alba-tercedor, Fernando E. Vega
    Abstract:

    Traditionally, the study of anatomy in insects has been based on dissection techniques. Micro-computed tomography (micro-CT) is an X-ray based technique that allows visualization of the internal anatomy of insects in situ and does not require dissections. We report on the use of micro-CT scans to study, in detail, the internal structures and organs of the coffee berry borer ( Hypothenemus Hampei ), the most damaging insect pest of coffee worldwide. Detailed images and videos allowed us to make the first description of the aedeagus and the first report of differences between the sexes based on internal anatomy (flight musculature, midgut shape, hindgut convolutions, brain shape and size) and external morphology (lateral outline of the pronotum and number of abdominal tergites). This study is the first complete micro-CT reconstruction of the anatomy of an insect and is also the smallest insect to have been evaluated in this way. High quality rendered images, and additional supplementary videos and 3D models are suitable for use with mobile devices and are useful tools for future research and as teaching aids.

  • Sterility of the coffee berry borer, Hypothenemus Hampei (Coleoptera: Curculionidae), caused by the nematode Metaparasitylenchus hypothenemi (Tylenchidae: Allantonematidae)
    Biocontrol Science and Technology, 2019
    Co-Authors: Alfredo Castillo, Félix Martínez, Jaime Gómez, Juan Cisneros, Fernando E. Vega
    Abstract:

    Metaparasitylenchus hypothenemi is an endoparasitic nematode that causes partial or total sterility of coffee berry borer (Hypothenemus Hampei) females, although the causes are unknown. Fecundity a...

  • Visualizing the Mesothoracic Spiracles in a Bark Beetle: Hypothenemus Hampei (Coleoptera: Curculionidae)
    Annals of The Entomological Society of America, 2015
    Co-Authors: Fernando E. Vega, Francisco Infante, Jorge Valdez-carrasco, Gary R. Bauchan, Roger Beaver
    Abstract:

    ABSTRACT In a low-temperature scanning electron microscopy study aimed at determining whether the coffee berry borer [Hypothenemus Hampei (Ferrari) (Coleoptera: Curculionidae)] possesses mycangia, we fortuitously detected the mesothoracic spiracles, which are usually concealed. The mesothoracic spiracles are ventrolaterally located in the intersegmental membranes between the prothorax and mesothorax, and contain a large number of setae. This is the first study that includes photographs of the mesothoracic spiracles in a bark beetle.

Soekadar Wiryadiputra - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Picung (Pangium Edule) Plant Extracts as a Botanical Pesticide on Mortality of Coffee Berry Borer (Hypothenemus Hampei)
    Pelita Perkebunan: a Coffee and Cocoa Research Journal, 2014
    Co-Authors: Soekadar Wiryadiputra
    Abstract:

    Coffee pests known as coffe berry borer (CBB, Hypothenemus Hampei) were main pests which decreasing the productivity of Indonesian coffee. One of pests controlling was done by insecticides. Generally, plant uses for insecticide show high security level, because the breaking molecule was easy as not dangerous compound. Pangium edule contains of flavanoide, cyanide acid and saponin had potential as an botanical insecticide. The purpose of this research was to prove the potential extract of seed and leaves of picung (Pangium edule) as an botanical insecticide for CBB. This research used a complete random design. There were 8 treatments with the concentration of the extract from leaves and seed of picung, one positive control treatment (Carbaril 0,02% formulation) and one negative control treatment (aquades). The treatment was repeated four times and carried out observation on every day until six days. The concentrations leaf and seed extracts were 1.0%, 2.5% and 5.0%. The result of the research show that between concentration applied the were no significant different and at observation six days after application the mortality of CBB only around 35 -40% on both extracts (ewater and methanol). The result of the research also show that there were no real differentiation between leaves and seed of picung. LT-50 values were 1.25% and 0.96%, for leaves and seed extract in water for six day observation. From this observation could be concluded that picung tree (Pangium edule) is not effective in the controlling CBB in the interval concentration applied and extraction method used. Key words: Botanical pesticide, picung tree, Pangium edule, mortality, Hypothenemus Hampei, water extraction, methanol extraction.

  • Distribution Pattern of Coffee Berry Borer (Hypothenemus Hampei) on Arabica and Robusta Coffee
    Pelita Perkebunan (a Coffee and Cocoa Research Journal), 2014
    Co-Authors: Soekadar Wiryadiputra
    Abstract:

    Coffee berry borer [CBB, Hypothenemus Hampei (Ferr.)] is the main pest on coffee causing a significant losses. Distribution pattern of the pest is not known deeply until now, especially in Indonesia. The data of distribution pattern of pest is very important in constructing the strategy of integrated pest management, especially to determine a sampling method for monitoring of the pest. This experiment aimed to reveal the distribution pattern of CBB both spatially and vertically. The experiment was conducted on Arabica and Robusta coffee, located in Kalibendo estate in Banyuwangi East Java. A plot with 400 (20 x 20) of coffee trees were observed for infestation and population of CBB, at four branches on south, north, east and west directions for each tree. Collected data were analyzed to obtain the value of mean, variance (=s2), variance/mean relationship (=I), index of Morisita (=Iδ), coefficient of Green (=Cx) and k exponent of Negative Binomial. Results of the experiment revealed that spatial distribution pattern of CBB, both on Arabica an Robusta coffee, as well as for infestation and population parameters, was fit with aggregated or clumped distribution. For vertical distribution, it inclined that CBB infestation and population in the lower part of coffee tree was higher than in central and upper part of coffee tree. Plenty of infested coffee berries leaved on soil surface may result in higher infestation and population in the lower part. Key words: Arabica coffee, Robusta coffee, Hypothenemus Hampei, spatial distribution, vertical distribution.

  • Pola Distribusi Hama Penggerek Buah Kopi (Hypothenemus Hampei) pada Kopi Arabika dan Robusta Distribution Pattern of Coffee Berry Borer (Hypothenemus Hampei) on Arabica and Robusta Coffee
    2014
    Co-Authors: Soekadar Wiryadiputra
    Abstract:

    Coffee berry borer (CBB, Hypothenemus Hampei (Ferr.)) is the main pest on coffee causing a significant losses. Distribution pattern of the pest is not known deeply until now, especially in Indonesia. The data of distribution pattern of pest is very important in constructing the strategy of integrated pest management, especially to determine a sampling method for monitoring of the pest. This experiment aimed to reveal the distribution pattern of CBB both spatially and vertically. The experiment was conducted on Arabica and Robusta coffee, located in Kalibendo estate in Banyuwangi East Java. A plot with 400 (20 x 20) of coffee trees were observed for infestation and population of CBB, at four

  • Keefektifan Insektisida Cyantraniliprole Terhadap Hama Penggerek Buah Kopi ( Hypothenemus Hampei) Pada Ko Pi Arabika ( Effectiveness Cyantraniliprole Against Coffee Berry Borer (Hypothenemus Hampei)on Arabica Coffee.
    Pelita Perkebunan (a Coffee and Cocoa Research Journal), 2012
    Co-Authors: Soekadar Wiryadiputra
    Abstract:

    A trial on cyantraniliprole 10% against coffee berry borer (Hypothenemus Hampei) has been conducted on arabica coffee at Kalibendo Estate, in Banyuwangi regency, East Java. The altitude of the estate is about 650 m above sea level (asl.) and belongs to B type of climate classification according to Schmidt and Ferguson. Composite variety of arabica coffee at about four years old planted at the location was used as plant materials. Five levels of cyantranilprole dosage and two compared insecticides i.e: carbaryl 85% and lamda cyhalothrine 25 g/L have been applied as treatments and each treatment is replicated four times. Infestation of coffee berry borer (CBB) has been observed on berries in the field as well as on harvested berries and green coffee. The results revealed that cyantraniliprole 10% was very effective in suppressing infestation and population of CBB on coffee berries in the field as well as on harvested parchment and green coffee. The dosage of 2,000 ml/ha was the most effective and the highest level of efficacy against CBB until the last observation during 14 weeks trial. Application of cyantraniliprole 10% also has increased the production of green coffee harvested. The highest increase occurred on the treatment of cyantraniliprole 10% with a dosage of 1000 ml/ha, which it reached 62.87% higher compared to untreated treatment. Carbaryl and lamda cyhalothrine have effectiveness and efficacy level lower than the highest dosage of cyantraniliprole 10%.

  • Use of Trapping for Controlling of Coffee Berry Borer (Hypothenemus Hampei)
    Pelita Perkebunan (a Coffee and Cocoa Research Journal), 2006
    Co-Authors: Soekadar Wiryadiputra
    Abstract:

    Studies on the field trapping of coffee berry borer (CBB, Hypothenemus Hampei) have been conducted to evaluate trap color, trap design and lures (attractant substances). The trials were conducted in a Robusta coffee plantation in East Java during August to December 2004. The trap color evaluated were red, orange, yellow, green and blue, set up in the coffee plantation using a four funnels trap. The traps installed on wood poles at a height of 175 cm above ground and placed among coffee trees. Observations were conducted every day for a week. Result of the study showed that the red and blue color traps captured CBB significantly higher than the others. During a week, the red color captured 1694 CBB adults while the blue one captured 1619 CBB adults. Peak number of captured CBB of the red and blue trapping occurred on the third day with the number of CBB of 416 and 395, respectively. In the evaluation of trapping design, four types of trapping were tried at the same location. The types of trapping were single funnel red trapping, four funnel red trapping, bottle trap with two straight opposite holes, and bottle trapping with two-zigzag holes. Bottle trapping was made of plastic bottle of 1.5-litre capacity, provided with two holes at its opposite wall. Setting up of the trapping in the field and the observation time were in the same way with the trial in trapping color evaluation. Results obtained indicated that the bottle trapping with two straight opposite holes captured the highest number of CBB followed by four funnel red trapping i.e. 547 and 69 per week, respectively. The peak number of trapped CBB occurred at the third day, as at the color trials. Furthermore, four lures have been evaluated int order to obtain the most effective substances for CBB trapping. Substances of A, B, C and D were set up in a coffee plantation using two types of trapping, four funnel red trapping and bottle trapping with two straight opposite holes. The results showed that the substances of B, C and D captured higher CBB adult than A. Nevertheless, this results was not fully convincing since the number of CBB trapped was low, which was due to low population of the CBB during the last trial. Key words: Coffee, trapping, coffee berry borer (CBB), Hypothenemus Hampei, trap color, trap desain, lure

Flávio Lemes Fernandes - One of the best experts on this subject based on the ideXlab platform.

  • Cyantraniliprole susceptibility baseline, resistance survey and control failure likelihood in the coffee berry borer Hypothenemus Hampei.
    Ecotoxicology and environmental safety, 2020
    Co-Authors: Nayara Cecília Rodrigues Costa, Eduardo Da Cruz Maduro Picelli, Fábio Maximiano De Andrade Silva, Alfredo Henrique Rocha Gonring, Raul Narciso C. Guedes, Mariana R. Durigan, Flávio Lemes Fernandes
    Abstract:

    Abstract Cyantraniliprole was recently registered for controlling the coffee berry borer Hypothenemus Hampei, the main coffee pest in the world. In this study, baseline determination and resistance monitoring to cyantraniliprole were carried out in Brazilian populations of H. Hampei. Evaluations were carried out for three years with representative field-collected populations from nine coffee-producing states in Brazil, using artificial diet containing the insecticide. The likelihood of control failure due to cyantraniliprole resistance was also determined. Populations from Campo do Meio, Linhares and Jau were more susceptible (

  • Chlorantraniliprole-mediated Effects on Survival, Walking Abilities, and Respiration in the Coffee Berry Borer, Hypothenemus Hampei
    Ecotoxicology and environmental safety, 2019
    Co-Authors: Angelica Plata-rueda, Nayara Cecília Rodrigues Costa, Raul Narciso C. Guedes, Luis Carlos Martínez, José Cola Zanuncio, Maria Elisa De Sena Fernandes, José Eduardo Serrão, Flávio Lemes Fernandes
    Abstract:

    Abstract Hypothenemus Hampei Ferrari (Coleoptera: Curculionidae) is the main pest of coffee crops, and effective methods for pest management are needed urgently. Bioassays were conducted to assess the effects of the insecticide chlorantraniliprole on H. Hampei adults. Toxicity, survivorship, larval production, respiration rate, and behavioral responses to six concentrations of chlorantraniliprole were evaluated. Chlorantraniliprole was toxic to H. Hampei (LD50 = 0.49 mg mL−1 and LD90 = 1.21 mg mL−1). Survivorship was 98% in adults not exposed to chlorantraniliprole, decreasing to 52% in insects exposed to LD50 and 2% in insects treated with LD90. H. Hampei showed reduced mobility on insecticide-treated surfaces. The insecticide promoted a decrease in the respiration rate of H. Hampei for up to 3 h after exposure, altering behavioral responses and locomotor activity. Chlorantraniliprole was shown to have lethal and sublethal effects on H. Hampei and, thus, can be used rotationally in integrated pest management programs to control of this pest in coffee crops and retard of insect resistance.