IAA Oxidase

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Ezzedine El Ferjani - One of the best experts on this subject based on the ideXlab platform.

  • effects of cadmium and copper on perOxidase nadh Oxidase and IAA Oxidase activities in cell wall soluble and microsomal membrane fractions of pea roots
    Journal of Plant Physiology, 2004
    Co-Authors: A. Chaoui, Brahim Jarrar, Ezzedine El Ferjani
    Abstract:

    Abstract Twelve-day-old seedlings of pea ( Pisum sativum L.) that were treated for 4 days by 20 and 100 μmol/l Cd(NO 3 ) 2 or CuSO 4 showed a growth reduction in all organs. From root protein extracts, the activities of guaiacol perOxidase (GPX; EC 1.11.1.7), ascorbate perOxidase (APX; EC 1.11.1.11), coniferyl alcohol perOxidase (CAPX), NADH Oxidase, and indole-3-acetic acid (IAA) Oxidase were measured in covalently—and ionically—bound cell wall, soluble, and microsomal membrane fractions. With the exception of 20 μmol/l Cu, metal treatments enhanced GPX activity in all fractions. Only IAA Oxidase activity was metal-elevated in the covalently bound cell wall fraction, while the ionic one showed Cd stimulation for all assayed enzymic activities. These effects were not entirely observed in Cu-treated plants, since APX and IAA Oxidase activities were only enhanced in this fraction. However, soluble extract showed stimulation of APX activity, while in the microsomal fraction metal exposure also increased the activities of CAPX and NADH Oxidase. Differential responses of root cell fractions to the presence of cadmium and copper ions are discussed in regard to the contribution of their enzymic capacities in antioxidant, lignification, and auxin degradation pathways. Comparisons between metals and dose effects are also underlined.

  • Effects of cadmium and copper on perOxidase, NADH Oxidase and IAA Oxidase activities in cell wall, soluble and microsomal membrane fractions of pea roots.
    Journal of Plant Physiology, 2004
    Co-Authors: A. Chaoui, Brahim Jarrar, Ezzedine El Ferjani
    Abstract:

    Twelve-day-old seedlings of pea (Pisum sativum L.) that were treated for 4 days by 20 and 100 micromol/l Cd(NO3)2 or CuSO4 showed a growth reduction in all organs. From root protein extracts, the activities of guaiacol perOxidase (GPX; EC 1.11.1.7), ascorbate perOxidase (APX; EC 1.11.1.11), coniferyl alcohol perOxidase (CAPX), NADH Oxidase, and indole-3-acetic acid (IAA) Oxidase were measured in covalently--and ionically--[symbol: see text] bound cell wall, soluble, and microsomal membrane fractions. With the exception of 20 micromol/l Cu, metal treatments enhanced GPX activity in all fractions. Only IAA Oxidase activity was metal-elevated in the covalently bound cell wall fraction, while the ionic one showed Cd stimulation for all assayed enzymic activities. These effects were not entirely observed in Cu-treated plants, since APX and IAA Oxidase activities were only enhanced in this fraction. However, soluble extract showed stimulation of APX activity, while in the microsomal fraction metal exposure also increased the activities of CAPX and NADH Oxidase. Differential responses of root cell fractions to the presence of cadmium and copper ions are discussed in regard to the contribution of their enzymic capacities in antioxidant, lignification, and auxin degradation pathways. Comparisons between metals and dose effects are also underlined.

A. Chaoui - One of the best experts on this subject based on the ideXlab platform.

  • effects of cadmium and copper on perOxidase nadh Oxidase and IAA Oxidase activities in cell wall soluble and microsomal membrane fractions of pea roots
    Journal of Plant Physiology, 2004
    Co-Authors: A. Chaoui, Brahim Jarrar, Ezzedine El Ferjani
    Abstract:

    Abstract Twelve-day-old seedlings of pea ( Pisum sativum L.) that were treated for 4 days by 20 and 100 μmol/l Cd(NO 3 ) 2 or CuSO 4 showed a growth reduction in all organs. From root protein extracts, the activities of guaiacol perOxidase (GPX; EC 1.11.1.7), ascorbate perOxidase (APX; EC 1.11.1.11), coniferyl alcohol perOxidase (CAPX), NADH Oxidase, and indole-3-acetic acid (IAA) Oxidase were measured in covalently—and ionically—bound cell wall, soluble, and microsomal membrane fractions. With the exception of 20 μmol/l Cu, metal treatments enhanced GPX activity in all fractions. Only IAA Oxidase activity was metal-elevated in the covalently bound cell wall fraction, while the ionic one showed Cd stimulation for all assayed enzymic activities. These effects were not entirely observed in Cu-treated plants, since APX and IAA Oxidase activities were only enhanced in this fraction. However, soluble extract showed stimulation of APX activity, while in the microsomal fraction metal exposure also increased the activities of CAPX and NADH Oxidase. Differential responses of root cell fractions to the presence of cadmium and copper ions are discussed in regard to the contribution of their enzymic capacities in antioxidant, lignification, and auxin degradation pathways. Comparisons between metals and dose effects are also underlined.

  • Effects of cadmium and copper on perOxidase, NADH Oxidase and IAA Oxidase activities in cell wall, soluble and microsomal membrane fractions of pea roots.
    Journal of Plant Physiology, 2004
    Co-Authors: A. Chaoui, Brahim Jarrar, Ezzedine El Ferjani
    Abstract:

    Twelve-day-old seedlings of pea (Pisum sativum L.) that were treated for 4 days by 20 and 100 micromol/l Cd(NO3)2 or CuSO4 showed a growth reduction in all organs. From root protein extracts, the activities of guaiacol perOxidase (GPX; EC 1.11.1.7), ascorbate perOxidase (APX; EC 1.11.1.11), coniferyl alcohol perOxidase (CAPX), NADH Oxidase, and indole-3-acetic acid (IAA) Oxidase were measured in covalently--and ionically--[symbol: see text] bound cell wall, soluble, and microsomal membrane fractions. With the exception of 20 micromol/l Cu, metal treatments enhanced GPX activity in all fractions. Only IAA Oxidase activity was metal-elevated in the covalently bound cell wall fraction, while the ionic one showed Cd stimulation for all assayed enzymic activities. These effects were not entirely observed in Cu-treated plants, since APX and IAA Oxidase activities were only enhanced in this fraction. However, soluble extract showed stimulation of APX activity, while in the microsomal fraction metal exposure also increased the activities of CAPX and NADH Oxidase. Differential responses of root cell fractions to the presence of cadmium and copper ions are discussed in regard to the contribution of their enzymic capacities in antioxidant, lignification, and auxin degradation pathways. Comparisons between metals and dose effects are also underlined.

Sumitra Chanda - One of the best experts on this subject based on the ideXlab platform.

  • Effects of Mercury and Chromium on PerOxidase and IAA Oxidase Enzymes in the Seedlings of Phaseolus vulgaris
    Turkish Journal of Biology, 2005
    Co-Authors: Nayna G. Parmar, Sumitra Chanda
    Abstract:

    Phaseolus seedlings (Phaseolus vulgaris L.) in light conditions were treated with 2 different concentrations of 2 heavy metals, mercury (0.05 mM and 0.4 mM) and chromium (0.5 and 1.0 mM). Mercury was given in the form of HgCl2 and chromium was given in the form of K2Cr2O7. PerOxidase activity was measured with 4 different hydrogen donors (ferulic acid, caffeic acid, pyrocatechol and pyrogallol). Both mercury and chromium inhibited root and hypocotyl length. The inhibition was about 50%-80%, depending on the concentration of the heavy metal. PerOxidase and IAA Oxidase activities showed a clear inverse relation with growth. Seedlings treated with a lower concentration of mercury had similar activities to those treated with higher concentrations of chromium. The role of perOxidase and IAA Oxidase activities in defense mechanisms in response to heavy metal toxicity is discussed.

  • Effect of salinity stress on growth, perOxidase and IAA Oxidase activities in vigna seedlings
    Acta Physiologiae Plantarum, 2002
    Co-Authors: D. S. Pujari, Sumitra Chanda
    Abstract:

    The present work was carried out with the aim of studying the effect of salinity stress on growth and IAA oxidizing system (i.e. perOxidase and IAA Oxidase) in vigna (Vigna unguiculata L.) seedlings. The seedlings were treated with two concentrations of sodium chloride (NaCl) 0.1 M and 0.25 M. Length, fresh and dry weight were the parameters considered for growth. Salinity effect was distinct in fresh weight and dry weight of different organs. PerOxidase and IAA Oxidase activities were measured at different time intervals for both cytoplasmic and wall bound fractions. PerOxidase activity was maximum at higher stress conditions bringing about the hypocotyl growth restriction. Thus there was a clear inverse correlation between elongation and perOxidase activity. IAA Oxidase activity also showed a similar trend for both cytoplasmic and wall bound fractions. The role of IAA oxidizing system in defense mechanism in response to salinity stress is discussed.

  • changes in perOxidase and IAA Oxidase activities during cell elongation in phaseolus hypocotyls
    Acta Physiologiae Plantarum, 1998
    Co-Authors: S B Bagatharia, Sumitra Chanda
    Abstract:

    Cytoplasmic and salt-extracted perOxidase and IAA Oxidase activities were studied in Phaseolus vulgaris hypocotyls treated with gibberellic acid (GA, 200 µM), naphthyl acetic acid (NAA, 100 µM) and distilled water control (DW). PerOxidase activity was assayed with four hydrogen donors during the initial phase of hypocotyl elongation. Though perOxidase activity showed a decreasing trend with time in all the hydrogen donors studied; considerable variation with different hydrogen donors was observed. NAA had maximum perOxidase activity as compared to DW or GA treatment. The activity showed a clear inverse correlation with hypocotyl growth. IAA Oxidase activity showed a similar trend with growth as perOxidase activity. A highly significant correlation was observed between perOxidase and IAA Oxidase activities and high molecular weight xyloglucan content (P<0.001). Finally, the possible role of perOxidase and IAA Oxidase activities in hypocotyl elongation growth is discussed.

  • Changes in perOxidase and IAA Oxidase activities during wheat grain development
    Plant Physiology and Biochemistry, 1997
    Co-Authors: Sumitra Chanda, Yash Dev Singh
    Abstract:

    Grain dry weight, water content and changes in the activities of soluble and salt-extracted (ionically bound) perOxidase and IAA Oxidase were investigated in three grains (differing in their final dry weight and position on the spike) of wheat (Triticum aestivum L.) cultivar Lok-1 during the entire period of grain development. Based on biphasic regression analysis of grain dry weight and water content data, the wheat grain development was divided into four phases: (a) cell division, (b) cell elongation, (c) dry matter accumulation, and (d) maturation. In all the three grains, soluble IAA Oxidase activity increased during the elongation phase while ionically bound activity increased during dry matter accumulation and maturation phases. Changes in ionically bound perOxidase activity showed inverse correlation with the process of cell elongation whereas no relationship between soluble perOxidase activity and grain development was discernible. The close correlation between cessation of elongation growth and increase in wall perOxidase and IAA Oxidase activities points to an important role of these enzymes in the termination of elongation phase, in wheat grain development.

Luo Jianping - One of the best experts on this subject based on the ideXlab platform.

  • study on the determination of IAA Oxidase activity by high performance capillary electrophoresis
    Journal of Anhui Agricultural Sciences, 2008
    Co-Authors: Wei Peng, Cha Xueqiang, Luo Jianping
    Abstract:

    [Objective] The aim of the research was to provide basis for further research and development of Dendrobium huoshanense. [Method] Taking the buffer system consisted of 80 mmol/L borax buffer and 15 mmol/L glucose solutions with pH value of 9.0 as the background electrolyte, the optimum conditions for determining the activity of indoleacetic acid (IAA) Oxidase were studied by high performance capillary electrophoresis technique. [Result] When the reaction was made for 80 min, the enzyme activity was reduced. When the substrate concentration was lower, the enzyme was increased with the increasing of the substrate concn. When the substrate concn. was more than 20 mg/L, the enzyme activity was not increased but maintained at the level of about 5.7 g/(mg·h). When pH value was about 6.0, the enzyme activity was highest. Temperature had greater effects on the enzyme activity. When the reaction temperature was 5 ℃, the enzyme activity was lower, then the enzyme activity was gradually increased with the increasing of temperature and it was obviously decreased after 30 ℃. When the temperature was 20-30 ℃, the activity of IAA Oxidase was highest. [Conclusion] When the temperature was 25 ℃, pH value was 6.0 and the substrate concn. was 20 mg/L, the activity of IAA Oxidase was the highest.

Wieslaw Nowakowski - One of the best experts on this subject based on the ideXlab platform.